Due to the increasing availability of whole slide scanners facilitating digitization of histopathological tissue, there is a strong demand for the development of computer based image analysis systems. In this work, the focus is on the segmentation of the glomeruli constituting a highly relevant structure in renal histopathology, which has not been investigated before in combination with CNNs. We propose two different CNN cascades for segmentation applications with sparse objects. These approaches are applied to the problem of glomerulus segmentation and compared with conventional fully-convolutional networks. Overall, with the best performing cascade approach, single CNNs are outperformed and a pixel-level Dice similarity coefficient of 0.90 is obtained. Combined with qualitative and further object-level analyses the obtained results are assessed as excellent also compared to recent approaches. In conclusion, we can state that especially one of the proposed cascade networks proved to be a highly powerful tool for segmenting the renal glomeruli providing best segmentation accuracies and also keeping the computing time at a low level.
Few approaches were proposed to perform segmentation of the glomeruli. @cite_12 introduced a separate detection and segmentation stage, both based on training a supervised classification model, specifically a linear support vector machine. For detection and segmentation different versions of histogram of oriented gradient descriptors were applied. Detection was performed by means of the sliding window approach. For segmentation, a polygon-fitting technique was utilized to determine the precise outline of the objects by adding a circularity constraint. For learning to distinguish between border region and non-border regions (and not between inside and outside of a glomerulus), this method requires highly precise annotations for training. This method was developed for a specific staining (desmin) which highlights cells in glomeruli.
@cite_12
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A new method for detecting rooftops in satellite images is presented. The proposed method is based on a combination of machine learning techniques, namely, k-means clustering and support vector machines (SVM). Firstly k-means clustering is used to segment the image into a set of rooftop candidates—these are homogeneous regions in the image which are potentially associated with rooftop areas. Next, the candidates are submitted to a classification stage which determines which amongst them correspond to “true” rooftops. To achieve improved accuracy, a novel two-pass classification process is used. In the first pass, a trained SVM is used in the normal way to distinguish between rooftop and nonrooftop regions. However, this can be a challenging task, resulting in a relatively high rate of misclassification. Hence, the second pass, which we call the “histogram method,” was devised with the aim of detecting rooftops which were missed in the first pass. The performance of the model is assessed both in terms of the percentage of correctly classified candidates as well as the accuracy of the estimated rooftop area. Background Understanding the cellular and molecular basis of tissue development and function requires analysis of individual cells while in their tissue context. Methods We developed software to find the optimum border around each cell (segmentation) from two-dimensional microscopic images of intact tissue. Samples were labeled with a fluorescent cell surface marker so that cell borders were brighter than elsewhere. The optimum border around each cell was defined as the border with an average intensity per unit length greater that any other possible border around that cell, and was calculated using the gray-weighted distance transform. Algorithm initiation requiring the user to mark two points per cell, one approximately in the center and the other on the border, ensured virtually 100 correct segmentation. Thereafter segmentation was automatic. Results The method was highly robust, because intermittent labeling of the cell borders, diffuse borders, and spurious signals away from the border do not significantly affect the optimum path. Computer-generated cells with increasing levels of added noise showed that the approach was accurate provided the cell could be detected visually. Conclusions We have developed a highly robust algorithm for segmenting images of surface-labeled cells, enabling accurate and quantitative analysis of individual cells in tissue. © 2005 International Society for Analytical Cytology Malaria detection through microscopic examination of stained blood smears is a diagnostic challenge that heavily relies on the expertise of trained microscopists. This paper presents an automated analysis method for detection and staging of red blood cells infected by the malaria parasite Plasmodium falciparum at trophozoite or schizont stage. Unlike previous efforts in this area, this study uses quantitative phase images of unstained cells. Erythrocytes are automatically segmented using thresholds of optical phase and refocused to enable quantitative comparison of phase images. Refocused images are analyzed to extract 23 morphological descriptors based on the phase information. While all individual descriptors are highly statistically different between infected and uninfected cells, each descriptor does not enable separation of populations at a level satisfactory for clinical utility. To improve the diagnostic capacity, we applied various machine learning techniques, including linear discriminant classification (LDC), logistic regression (LR), and k-nearest neighbor classification (NNC), to formulate algorithms that combine all of the calculated physical parameters to distinguish cells more effectively. Results show that LDC provides the highest accuracy of up to 99.7 in detecting schizont stage infected cells compared to uninfected RBCs. NNC showed slightly better accuracy (99.5 ) than either LDC (99.0 ) or LR (99.1 ) for discriminating late trophozoites from uninfected RBCs. However, for early trophozoites, LDC produced the best accuracy of 98 . Discrimination of infection stage was less accurate, producing high specificity (99.8 ) but only 45.0 -66.8 sensitivity with early trophozoites most often mistaken for late trophozoite or schizont stage and late trophozoite and schizont stage most often confused for each other. Overall, this methodology points to a significant clinical potential of using quantitative phase imaging to detect and stage malaria infection without staining or expert analysis. We present a novel method to recognise planar structures in a single image and estimate their 3D orientation. This is done by exploiting the relationship between image appearance and 3D structure, using machine learning methods with supervised training data. As such, the method does not require specific features or use geometric cues, such as vanishing points. We employ general feature representations based on spatiograms of gradients and colour, coupled with relevance vector machines for classification and regression. We first show that using hand-labelled training data, we are able to classify pre-segmented regions as being planar or not, and estimate their 3D orientation. We then incorporate the method into a segmentation algorithm to detect multiple planar structures from a previously unseen image. By combining Histograms of Oriented Gradients (HOG) and Local Binary Pattern (LBP) as the feature set, we propose a novel human detection approach capable of handling partial occlusion. Two kinds of detectors, i.e., global detector for whole scanning windows and part detectors for local regions, are learned from the training data using linear SVM. For each ambiguous scanning window, we construct an occlusion likelihood map by using the response of each block of the HOG feature to the global detector. The occlusion likelihood map is then segmented by Mean-shift approach. The segmented portion of the window with a majority of negative response is inferred as an occluded region. If partial occlusion is indicated with high likelihood in a certain scanning window, part detectors are applied on the unoccluded regions to achieve the final classification on the current scanning window. With the help of the augmented HOG-LBP feature and the global-part occlusion handling method, we achieve a detection rate of 91.3 with FPPW= 10−6, 94.7 with FPPW= 10−5, and 97.9 with FPPW= 10−4 on the INRIA dataset, which, to our best knowledge, is the best human detection performance on the INRIA dataset. The global-part occlusion handling method is further validated using synthesized occlusion data constructed from the INRIA and Pascal dataset. In this paper, we present a computer vision based system for fast robust Traffic Sign Detection and Recognition (TSDR), consisting of three steps. The first step consists on image enhancement and thresholding using the three components of the Hue Saturation and Value (HSV) space. Then we refer to distance to border feature and Random Forests classifier to detect circular, triangular and rectangular shapes on the segmented images. The last step consists on identifying the information included in the detected traffic signs. We compare four features descriptors which include Histogram of Oriented Gradients (HOG), Gabor, Local Binary Pattern (LBP), and Local Self-Similarity (LSS). We also compare their different combinations. For the classifiers we have carried out a comparison between Random Forests and Support Vector Machines (SVMs). The best results are given by the combination HOG with LSS together with the Random Forest classifier. The proposed method has been tested on the Swedish Traffic Signs Data set and gives satisfactory results. Logos are specially designed marks that identify goods, services, and organizations using distinguished characters, graphs, signals, and colors. Identifying logos can facilitate scene understanding, intelligent navigation, and object recognition. Although numerous logo recognition methods have been proposed for printed logos, a few methods have been specifically designed for logos in photos. Furthermore, most recognition methods use codebook-based approaches for the logos in photos. A codebook-based method is concerned with the generation of visual words for all the logo models. When new logos are added, the codebook reconstruction is required if effectiveness is a crucial factor. Moreover, logo detection in natural scenes is difficult because of perspective tilt and non-rigid deformation. Therefore, this study develops an extendable, but discriminating, model-based logo detection method. The proposed logo detection method is based on a support vector machine (SVM) using edge-based histograms of oriented gradient (HOGE) as features through multi-scale sliding window scanning. Thereafter, anti-distortion affine scale invariant feature transform (ASIFT) is used for logo verification with constraints on the ASIFT matching pairs and neighbors. The experimental results using the public Flickr-Logo database confirm that the proposed method has a higher retrieval and precision accuracy compared to existing model-based methods. The standard chronic wound assessment method based on visual examination is potentially inaccurate and also represents a significant clinical workload. Hence, computer-based systems providing quantitative wound assessment may be valuable for accurately monitoring wound healing status, with the wound area the best suited for automated analysis. Here, we present a novel approach, using support vector machines (SVM) to determine the wound boundaries on foot ulcer images captured with an image capture box, which provides controlled lighting and range. After superpixel segmentation, a cascaded two-stage classifier operates as follows: in the first stage, a set of k binary SVM classifiers are trained and applied to different subsets of the entire training images dataset, and incorrectly classified instances are collected. In the second stage, another binary SVM classifier is trained on the incorrectly classified set. We extracted various color and texture descriptors from superpixels that are used as input for each stage in the classifier training. Specifically, color and bag-of-word representations of local dense scale invariant feature transformation features are descriptors for ruling out irrelevant regions, and color and wavelet-based features are descriptors for distinguishing healthy tissue from wound regions. Finally, the detected wound boundary is refined by applying the conditional random field method. We have implemented the wound classification on a Nexus 5 smartphone platform, except for training which was done offline. Results are compared with other classifiers and show that our approach provides high global performance rates (average sensitivity = 73.3 , specificity = 94.6 ) and is sufficiently efficient for a smartphone-based image analysis. Data-driven approaches for edge detection have proven effective and achieve top results on modern benchmarks. However, all current data-driven edge detectors require manual supervision for training in the form of hand-labeled region segments or object boundaries. Specifically, human annotators mark semantically meaningful edges which are subsequently used for training. Is this form of strong, highlevel supervision actually necessary to learn to accurately detect edges? In this work we present a simple yet effective approach for training edge detectors without human supervision. To this end we utilize motion, and more specifically, the only input to our method is noisy semi-dense matches between frames. We begin with only a rudimentary knowledge of edges (in the form of image gradients), and alternate between improving motion estimation and edge detection in turn. Using a large corpus of video data, we show that edge detectors trained using our unsupervised scheme approach the performance of the same methods trained with full supervision (within 3-5 ). Finally, we show that when using a deep network for the edge detector, our approach provides a novel pre-training scheme for object detection. Data-driven approaches for edge detection have proven effective and achieve top results on modern benchmarks. However, all current data-driven edge detectors require manual supervision for training in the form of hand-labeled region segments or object boundaries. Specifically, human annotators mark semantically meaningful edges which are subsequently used for training. Is this form of strong, high-level supervision actually necessary to learn to accurately detect edges? In this work we present a simple yet effective approach for training edge detectors without human supervision. To this end we utilize motion, and more specifically, the only input to our method is noisy semi-dense matches between frames. We begin with only a rudimentary knowledge of edges (in the form of image gradients), and alternate between improving motion estimation and edge detection in turn. Using a large corpus of video data, we show that edge detectors trained using our unsupervised scheme approach the performance of the same methods trained with full supervision (within 3-5 ). Finally, we show that when using a deep network for the edge detector, our approach provides a novel pre-training scheme for object detection. Abstract We present a machine learning approach called shape regression machine (SRM) for efficient segmentation of an anatomic structure that exhibits a deformable shape in a medical image, e.g., left ventricle endocardial wall in an echocardiogram. The SRM achieves efficient segmentation via statistical learning of the interrelations among shape, appearance, and anatomy , which are exemplified by an annotated database. The SRM is a two-stage approach. In the first stage that estimates a rigid shape to solve an automatic initialization problem, it derives a regression solution to object detection that needs just one scan in principle and a sparse set of scans in practice, avoiding the exhaustive scanning required by the state-of-the-art classification-based detection approach while yielding comparable detection accuracy. In the second stage that estimates the nonrigid shape, it again learns a nonlinear regressor to directly associate nonrigid shape with image appearance. The underpinning of both stages is a novel image-based boosting ridge regression (IBRR) method that enables multivariate, nonlinear modeling and accommodates fast evaluation. We demonstrate the efficiency and effectiveness of the SRM using experiments on segmenting the left ventricle endocardium from a B-mode echocardiogram of apical four chamber view. The proposed algorithm is able to automatically detect and accurately segment the LV endocardial border in about 120 ms . We propose a novel deep neural network architecture for semi-supervised semantic segmentation using heterogeneous annotations. Contrary to existing approaches posing semantic segmentation as a single task of region-based classification, our algorithm decouples classification and segmentation, and learns a separate network for each task. In this architecture, labels associated with an image are identified by classification network, and binary segmentation is subsequently performed for each identified label in segmentation network. The decoupled architecture enables us to learn classification and segmentation networks separately based on the training data with image-level and pixel-wise class labels, respectively. It facilitates to reduce search space for segmentation effectively by exploiting class-specific activation maps obtained from bridging layers. Our algorithm shows outstanding performance compared to other semi-supervised approaches even with much less training images with strong annotations in PASCAL VOC dataset. We propose a novel deep neural network architecture for semi-supervised semantic segmentation using heterogeneous annotations. Contrary to existing approaches posing semantic segmentation as a single task of region-based classification, our algorithm decouples classification and segmentation, and learns a separate network for each task. In this architecture, labels associated with an image are identified by classification network, and binary segmentation is subsequently performed for each identified label in segmentation network. The decoupled architecture enables us to learn classification and segmentation networks separately based on the training data with image-level and pixel-wise class labels, respectively. It facilitates to reduce search space for segmentation effectively by exploiting class-specific activation maps obtained from bridging layers. Our algorithm shows outstanding performance compared to other semi-supervised approaches with much less training images with strong annotations in PASCAL VOC dataset. Systemic Lupus Erythematosus involvement of the kidneys is associated with high morbidity and mortality. Inter-observer variability can also lead to an incorrect assessment of the disease. Computer aided image analysis can potentially be used to advance the understanding, diagnosis and treatment of Lupus. Our research focuses on the image analysis of kidney biopsies for quantifying tissue characteristics relevant to Lupus. In this paper we describe an algorithm for identifying glomeruli in H&E stained mouse renal biopsies. The proposed approach uses color segmentation to identify all objects in the image that potentially correspond to the Bowman's space around the glomeruli. This is followed by a perceptual grouping procedure to group objects based on their size, location and orientation. Voting in each column around an initial peak is considered to be a random variable.The optimal ? is determined by fitting and minimizing a 2nd-order curve.The optimal ? is determined by fitting and interpolating a sine curve.We calculate voting boundaries instead of searching for non-zero voting cells.The endpoint coordinates are determined by fitting instead of by solving equations. Line segment detection is a fundamental procedure in computer vision, pattern recognition, or image analysis applications. This paper proposes a statistical method based on the Hough transform for line segment detection by considering quantization error, image noise, pixel disturbance, and peak spreading, also taking the choice of the coordinate origin into account.A random variable is defined in each column in a peak region. Statistical means and statistical variances are calculated; the statistical non-zero cells are analyzed and computed. The normal angle is determined by minimizing the function which fits the statistical variances; the normal distance is calculated by interpolating the function which fits the statistical means. Endpoint coordinates of a detected line segment are determined by fitting a sine curve (rather than searching for the first and last non-zero voting cells, and solving equations containing coordinates of such cells).Experimental results on simulated data and real world images validate the performance of the proposed method for line segment detection. Semantic segmentation and object detection are nowadays dominated by methods operating on regions obtained as a result of a bottom-up grouping process (segmentation) but use feature extractors developed for recognition on fixed-form (e.g. rectangular) patches, with full images as a special case. This is most likely suboptimal. In this paper we focus on feature extraction and description over free-form regions and study the relationship with their fixed-form counterparts. Our main contributions are novel pooling techniques that capture the second-order statistics of local descriptors inside such free-form regions. We introduce second-order generalizations of average and max-pooling that together with appropriate non-linearities, derived from the mathematical structure of their embedding space, lead to state-of-the-art recognition performance in semantic segmentation experiments without any type of local feature coding. In contrast, we show that codebook-based local feature coding is more important when feature extraction is constrained to operate over regions that include both foreground and large portions of the background, as typical in image classification settings, whereas for high-accuracy localization setups, second-order pooling over free-form regions produces results superior to those of the winning systems in the contemporary semantic segmentation challenges, with models that are much faster in both training and testing. This paper presents SceneCut, a novel approach to jointly discover previously unseen objects and non-object surfaces using a single RGB-D image. SceneCut's joint reasoning over scene semantics and geometry allows a robot to detect and segment object instances in complex scenes where modern deep learning-based methods either fail to separate object instances, or fail to detect objects that were not seen during training. SceneCut automatically decomposes a scene into meaningful regions which either represent objects or scene surfaces. The decomposition is qualified by an unified energy function over objectness and geometric fitting. We show how this energy function can be optimized efficiently by utilizing hierarchical segmentation trees. Moreover, we leverage a pre-trained convolutional oriented boundary network to predict accurate boundaries from images, which are used to construct high-quality region hierarchies. We evaluate SceneCut on several different indoor environments, and the results show that SceneCut significantly outperforms all the existing methods. Most of the leading Convolutional Neural Network (CNN) models for semantic segmentation exploit a large number of pixel–level annotations. Such a human based labeling requires a considerable effort that complicates the creation of large–scale datasets. In this paper, we propose a deep learning approach that uses bounding box annotations to train a semantic segmentation network. Indeed, the bounding box supervision, even though less accurate, is a valuable alternative, effective in reducing the dataset collection costs. The proposed method is based on a two stage training procedure: first, a deep neural network is trained to distinguish the relevant object from the background inside a given bounding box; then, the output of the network is used to provide a weak supervision for a multi–class segmentation CNN. The performances of our approach have been assessed on the Pascal–VOC 2012 segmentation dataset, obtaining competitive results compared to a fully supervised setting. We address the problem of object detection and segmentation using global holistic properties of object shape. Global shape representations are highly susceptible to clutter inevitably present in realistic images, and thus can be applied robustly only using a precise segmentation of the object. To this end, we propose a figure ground segmentation method for extraction of image regions that resemble the global properties of a model boundary structure and are perceptually salient. Our shape representation, called the chordiogram, is based on geometric relationships of object boundary edges, while the perceptual saliency cues we use favor coherent regions distinct from the background. We formulate the segmentation problem as an integer quadratic program and use a semidefinite programming relaxation to solve it. The obtained solutions provide a segmentation of the object as well as a detection score used for object recognition. Our single-step approach achieves state-of-the-art performance on several object detection and segmentation benchmarks. Scene text detection and segmentation are two important and challenging research problems in the field of computer vision. This paper proposes a novel method for scene text detection and segmentation based on cascaded convolution neural networks (CNNs). In this method, a CNN-based text-aware candidate text region (CTR) extraction model (named detection network, DNet) is designed and trained using both the edges and the whole regions of text, with which coarse CTRs are detected. A CNN-based CTR refinement model (named segmentation network, SNet) is then constructed to precisely segment the coarse CTRs into text to get the refined CTRs. With DNet and SNet, much fewer CTRs are extracted than with traditional approaches while more true text regions are kept. The refined CTRs are finally classified using a CNN-based CTR classification model (named classification network, CNet) to get the final text regions. All of these CNN-based models are modified from VGGNet-16. Extensive experiments on three benchmark data sets demonstrate that the proposed method achieves the state-of-the-art performance and greatly outperforms other scene text detection and segmentation approaches.