Biological membranes are one of the most basic structures and regions of interest in cell biology. In the study of membranes, segment extraction is a well-known and difficult problem because of impeding noise, directional and thickness variability, etc. Recent advances in electron microscopy membrane segmentation are able to cope with such difficulties by training convolutional neural networks. However, because of the massive amount of features that have to be extracted while propagating forward, the practical usability diminishes, even with state-of-the-art GPU's. A significant part of these network features typically contains redundancy through correlation and sparsity. In this work, we propose a pruning method for convolutional neural networks that ensures the training loss increase is minimized. We show that the pruned networks, after retraining, are more efficient in terms of time and memory, without significantly affecting the network accuracy. This way, we manage to obtain real-time membrane segmentation performance, for our specific electron microscopy setup.
Model-driven segmentation methods model the image and object of interest through energy functions by using a priori knowledge: active contours @cite_17 @cite_6 and graph-based methods @cite_3 @cite_0 . Even though it is possible to obtain high quality results using these techniques, they tend to be highly parameter dependent and are hard to generalize because of the specific image and object assumptions that were made.
@cite_0 @cite_3 @cite_6 @cite_17
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In many neurophysiological studies, understanding the neuronal circuitry of the brain requires detailed 3D models of the nerve cells and their synapses. Typically, researchers build the 3D models by manually tracing the 2D cross-sectional profiles of the 3D structures from serial electron micrograph (EM) stacks and then construct the models from these 2D contours. While current computer-aided techniques can reduce the tracing time, they often require extensive user interaction. We propose a segmentation framework to extract the 2D profiles that is both fast and requires a minimal amount of user interaction. The framework uses graph cuts to minimize an energy defined over the image intensity and the flux of the intensity gradient field. Furthermore, to correct segmentation errors, our framework allows for efficient and intuitive editing of the initial results. The ability to constrain the geometry of deformable models for image segmentation can be useful when information about the expected shape or positioning of the objects in a scene is known a priori. An example of this occurs when segmenting neural cross sections in electron microscopy. Such images often contain multiple nested boundaries separating regions of homogeneous intensities. For these applications, multiphase level sets provide a partitioning framework that allows for the segmentation of multiple deformable objects by combining several level set functions. Although there has been much effort in the study of statistical shape priors that can be used to constrain the geometry of each partition, none of these methods allow for the direct modeling of geometric arrangements of partitions. In this paper, we show how to define elastic couplings between multiple level set functions to model ribbon-like partitions. We build such couplings using dynamic force fields that can depend on the image content and relative location and shape of the level set functions. To the best of our knowledge, this is the first work that shows a direct way of geometrically constraining multiphase level sets for image segmentation. We demonstrate the robustness of our method by comparing it with previous level set segmentation methods. Electron microscopy is an important modality for the analysis of neuronal structures in neurobiology. We address the problem of tracking axons across large distances in volumes acquired by serial block-face scanning electron microscopy (SBFSEM). Tracking, for this application, is defined as the segmentation of an axon that spans a volume using similar features between slices. This is a challenging problem due to the small cross-sectional size of axons and the low signal-to-noise ratio in our SBFSEM images. A carefully engineered algorithm using Kalman-snakes and optical flow computation is presented. Axon tracking is initialized with user clicks or automatically using the watershed segmentation algorithm, which identifies axon centers. Multiple axons are tracked from slice to slice through a volume, updating the positions and velocities in the model and providing constraints to maintain smoothness between slices. Validation results indicate that this algorithm can significantly speed up the task of manual axon tracking.