Creatine ingestion augments dietary carbohydrate mediated muscle glycogen supercompensation during the initial 24 h of recovery following prolonged exhaustive exercise in humans
Muscle glycogen availability can limit endurance exercise performance. We previously demonstrated 5 days of creatine (Cr) and carbohydrate (CHO) ingestion augmented post exercise muscle glycogen storage compared to CHO feeding alone in healthy volunteers. Here, we aimed to characterise the time course of this Cr induced response under more stringent and controlled experimental conditions and identify potential mechanisms underpinning this phenomenon. Fourteen healthy, male volunteers cycled to exhaustion at 70 VO2peak. Muscle biopsies were obtained at rest immediately post exercise and after 1, 3 and 6 days of recovery, during which Cr or placebo supplements (20 g day 1) were ingested along with a prescribed high CHO diet (37.5 kcal kg body mass 1 day 1, >80 calories CHO) Oral glucose tolerance tests (oral GTT) were performed pre exercise and after 1, 3 and 6 days of Cr and placebo supplementation. Exercise depleted muscle glycogen content to the same extent in both treatment groups. Creatine supplementation increased muscle total Cr, free Cr and phosphocreatine (PCr) content above placebo following 1, 3 and 6 days of supplementation (all P 0.05) Creatine supplementation also increased muscle glycogen content noticeably above placebo after 1 day of supplementation (P 0.05) which was sustained thereafter. This study confirmed dietary Cr augments post exercise muscle glycogen super compensation, and demonstrates this occurred during the initial 24 h of post exercise recovery (when muscle total Cr had increased by <10 This marked response ensued without apparent treatment differences in muscle insulin sensitivity (oral GTT, muscle GLUT4 mRNA) osmotic stress (muscle c fos and HSP72 mRNA) or muscle cell volume (muscle water content) responses, such that another mechanism must be causative.
Amino Acids