[Protective effect and mechanism of compound Ginkgo biloba granules on oxidative stress injury of HUVEC]
To reveal the protective and anti apoptosis effect of compound Ginkgo biloba granules on oxidative stress injury of human umbilical vein endothelial cells (HUVEC) Negative control group, H2O2 model group and 4 drug pretreatment groups (80, 160, 320, 640 mg* L 1) were established. The cell proliferation, morphological changes in each group after oxidative stress injury was detected by MTT assay and through microscope observation respectively. The content of LDH, MDA, SOD and NO and SOD activity in supernatant were detected to judge the protection effect of the drugs on endothelial cells. The protective effect on HUVEC apoptosis was analyzed by Caspase 3 activity test and Annexin V FITC/PI staining. Western blot was used to observe the expression of apoptosis related proteins Bcl 2 and Bax. Results showed that 1 200 mmol* L 1 H2O2 can induce oxidative stress injury in endothelial cells and reduce the cell survival rate; cell proliferation inhibition degree is positively correlated with the effect time of H2O2. Besides, 80, 160, 320 640 mg*L 1 compound Ginkgo biloba granules can protect HUVEC from oxidative stress injury, recover the normal proliferation level of cells, improve their state, prohibit cell apoptosis, and can up regulate and down regulate the expression level of Bcl 2 and Bax respectively. In conclusion, compound G. biloba granules can protect HUVEC from the oxidative stress injury induced by H2O2, its mechanism may be correlated with inhibition of the mitochondrial apoptotic pathway in HUVEC.
Zhongguo Zhong yao za zhi Zhongguo zhongyao zazhi China journal of Chinese materia medica