miR 411 5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2.
miR 411 5p (previously called miR 411) is severely involved in human diseases, however, the relationship between miR 411 5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR 411 5p was downregulated in breast cancer tissues compared with their matched adjacent non neoplastic tissues. In addition, the expression of miR 411 5p was also lower in breast cancer cell lines in contrast with MCF 10A. Moreover, we investigated the target and mechanism of miR 411 5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR 411 5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR 411 5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR 411 5p related mechanism in the progression of breast cancer and, miR 411 5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy.
Biochemical and biophysical research communications