Serratia aquatilis sp. nov. isolated from drinking water systems.
A cream white pigmented, oxidase negative bacterium (strain 2015 2462 01T) isolated from a drinking water system, was investigated in detail to determine its taxonomic position. Cells of the isolate were rod shaped and stained Gram negative. A comparison of the 16S rRNA gene sequence of strain 2015 2462 01T with sequences of the type strains of closely related species of the genus Serratia revealed highest similarity to Serratia fonticola (98.4 Serratia proteamaculans (97.8 Serratia liquefaciens and Serratia grimesii (both 97.7 16S rRNA gene sequence similarities to all other Serratia species were below 97.4 Multilocus sequence analysis (MLSA) on the basis of concatenated partial gyrB, rpoB, infB and atpD gene sequences showed a clear distinction of strain 2015 2462 01T from the type strains of the closest related Serratia species. The fatty acid profile of the strain consisted of C16 1 o7c, C16 0; C14 0 and C14 0 3 OH/iso C16 1 I as major components. DNA DNA hybridizations between 2015 2462 01T and S. fonticola ATCC 29844T resulted in a relatedness value of 27 (reciprocal 20 This DNA DNA hybridization result in combination with the MLSA results and the differential biochemical properties indicated that strain 2015 2462 01T represents a novel species of the genus Serratia, for which the name Serratia aquatilis sp. nov. is proposed. The type strain is 2015 2462 01T LMG 29119T CCM 8626T)
International journal of systematic and evolutionary microbiology