A point spread function aware filtered backprojection algorithm for focal plane scanning optical projection tomography
Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in blur in the reconstructed image. In this paper, we present a modified filtered backprojection method suitable for focal plane scanning optical projection tomography (FPS OPT) where each projection is obtained by scanning through focal planes during collection. We show that FPS OPT has an exact inversion formula akin to a filtered backprojection, but that incorporates the system's point spread function to recover a deblurred 3D volume. With simulations, we demonstrate that FPS OPT permits the use of high numerical aperture objectives that lead to more accurate images. We further illustrate the technique on experimentally acquired data from a fluorescently labeled zebrafish larva, which shows that our approach reduces out of focus blur.
2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI)