In vitro antifungal activity of extracts obtained from Hypericum perforatum adventitious roots cultured in a mist bioreactor against planktonic cells and biofilm of Malassezia furfur
Xanthone rich extracts from Hypericum perforatum root cultures grown in a Mist Bioreactor as antifungal agents against Malassezia furfur. Extracts of Hypericum perforatum roots grown in a bioreactor showed activity against planktonic cells and biofilm of Malassezia furfur. Dried biomass, obtained from roots grown under controlled conditions in a ROOTec mist bioreactor, has been extracted with solvents of increasing polarity (i.e. chloroform, ethyl acetate and methanol) The methanolic fraction was the richest in xanthones (2.86 0.43 mg g 1 DW) as revealed by HPLC. The minimal inhibitory concentration of the methanol extract against M. furfur planktonic cells was 16 mg mL 1. The inhibition percentage of biofilm formation, at a concentration of 16 mg mL 1, ranged from 14% to 39% The results show that H. perforatum root extracts could be used as new antifungal agents in the treatment of Malassezia infections.
Natural product research