Dioscin alleviates dimethylnitrosamine induced acute liver injury through regulating apoptosis, oxidative stress and inflammation.
In our previous study, the effects of dioscin against alcohol carbon tetrachloride and acetaminophen induced liver damage have been found. However, the activity of it against dimethylnitrosamine (DMN) induced acute liver injury remained unknown. In the present study, dioscin markedly decreased serum ALT and AST levels, significantly increased the levels of SOD, GSH Px, GSH, and decreased the levels of MDA, iNOS and NO. Mechanism study showed that dioscin significantly decreased the expression levels of IL 1b, IL 6, TNF a, IkBa, p50 and p65 through regulating TLR4/MyD88 pathway to rehabilitate inflammation. In addition, dioscin markedly up regulated the expression levels of SIRT1, HO 1, NQO1, GST and GCLM through increasing nuclear translocation of Nrf2 against oxidative stress. Furthermore, dioscin significantly decreased the expression levels of FasL, Fas, p53, Bak, Caspase 3/9, and upregulated Bcl 2 level through decreasing IRF9 level against apoptosis. In conclusion, dioscin showed protective effect against DMN induced acute liver injury via ameliorating apoptosis, oxidative stress and inflammation, which should be developed as a new candidate for the treatment of acute liver injury in the future.
Environmental toxicology and pharmacology