The two faced T cell epitope Examining the host microbe interface with JanusMatrix
JanusMatrix Website Figure 1. JanusMatrix separates the amino acid sequence of T cell epitopes into TCR facing residues and HLA binding cleft facing residues, then compares the TCR face to other putative T cell epitopes. JanusMatrix: Web based interface with query and analysis tools. Integrates genomic sequences, immunological data, and applied computational epitope prediction and analysis. Predicts Class I and Class II epitopes using EpiMatrix, compares TCR facing residues of the predicted epitopes against databases defined by the user. Generates multiple outputs including summaries, predicted epitopes, cross reactivity across multiple databases, cross reactivity annotation, among others. Outputs are interactive and can be downloaded. Cytoscape based approach to visualize related epitope networks. XR epitopes described in tabular format, patterns visualized with " epitope networks " using Cytoscape. Epitope networks: nodes for epitopes and the proteins that contain them, lines between XR epitope pairs and between proteins and their constituent epitopes. Individual epitope from source (9 mer) TCR XR 9 mer (epitope) Protein in HM, HP, HG containing XR epitope Source peptide or protein (may have multiple epitopes