Highly Effective Dual Function Near Infrared (NIR) Photosensitizer for Fluorescence Imaging and Photodynamic Therapy (PDT) of Cancer.
We report herein the synthesis and biological efficacy of near infrared (NIR) bacteriochlorin analogues: 3 (1' butyloxy)ethyl 3 deacetyl bacteriopurpurin 18 N butylimide methyl ester (3) and the corresponding carboxylic acid 10. In in vitro assays, compared to its methyl ester analogue 3, the corresponding carboxylic acid derivative 10 showed higher photosensitizing efficacy. However, due to drastically different pharmacokinetics in vivo, the PS 3 (HPLC purity >99% showed higher tumor uptake and long term tumor cure than 10 (HPLC purity >96.5% in BALB/c mice bearing Colon 26 tumors. Isomerically pure R and S isomers of 3 (3a and 3b, purity by HPLC 99% under similar treatment parameters showed identical efficacy in vitro and in vivo. In addition, photosensitizer (PS) 3 showed limited skin phototoxicity and provides an additional advantage over the clinically approved chemically complex hematoporphyrin derivative as well as other porphyrin based PDT agents, which makes 3 a promising dual function agent for fluorescence guided surgery with an option of phototherapy of cancer.
Journal of medicinal chemistry