A comparative study of conventional and supercritical fluid extraction methods for the recovery of secondary metabolites from Syzygium campanulatum Korth
Syzygium campanulatum Korth is a plant, which is a rich source of secondary metabolites (especially flavanones, chalcone, and triterpenoids) In our present study, three conventional solvent extraction (CSE) techniques and supercritical fluid extraction (SFE) techniques were performed to achieve a maximum recovery of two flavanones, chalcone, and two triterpenoids from S. campanulatum leaves. Furthermore, a Box Behnken design was constructed for the SFE technique using pressure, temperature, and particle size as independent variables, and yields of crude extract, individual and total secondary metabolites as the dependent variables. In the CSE procedure, twenty extracts were produced using ten different solvents and three techniques (maceration, soxhletion, and reflux) An enriched extract of five secondary metabolites was collected using n hexane:methanol (1:1) soxhletion. Using food grade ethanol as a modifier, the SFE methods produced a higher recovery (25.5% 84.9% of selected secondary metabolites as compared to the CSE techniques (0.92% 66.00%.Zhong Wen Gai Yao Mu De Xun Qiu Yi Chong Zui Jia De Zhong Hua Pu Tao Ci Sheng Dai Xie Chan Wu De Cui Qu Fang Fa ,Yi Shi Xian Zai Gong Ye Shang De Ying Yong Chuang Xin Dian Quan Mian Shen Ru Di Bi Jiao Chuan Tong Rong Ji Ti Qu Fa He Chao Lin Jie Liu Ti Cui Qu Fa Dui Zhong Hua Pu Tao De Zhu Yao Ci Sheng Dai Xie Chan Wu De Hui Shou Lu Fang Fa Zai Chao Lin Jie Liu Ti Cui Qu Fa Zhong ,Ying Yong Box Behnken She Ji Yi Ya Li Wen Du He Cui Qu Li Jing Da Xiao Wei Zi Bian Liang ,Yi Cu Ti Qu Wu Ge Bie De He Zong De Ci Sheng Dai Xie Chan Wu De Bai Fen Bi Chan Liang Wei Yin Bian Liang Ling Wai ,Huan Yan Jiu Liao Diao Jie Ji Yi Chun Dui Chan Liang De Ying Xiang Zai Chuan Tong Rong Ji Ti Qu Zhong ,Jie He Shi Chong Bu Tong De Rong Ji He San Bu Tong De Ji Zhu (Jin Ti Suo Shi He Hui Liu )Chan Sheng Er Shi Chong Bu Tong De Ti Qu Chan Wu Tong Guo Gao Xiao Ye Xiang Se Pu Fa Jian Ce Bu Tong De Ti Qu Fang Fa Dui Zhong Hua Pu Tao De Zhu Yao Ci Sheng Dai Xie Chan Wu De Hui Shou Lu Jie Lun Chao Lin Jie Liu Ti Cui Qu Fa You Yu Chuan Tong Rong Ji Ti Qu Fa ,Sheng Cheng Ben You Sheng Shi Yin Ci Zai Gong Ye Shi Yong Shang ,Chao Lin Jie Liu Ti Cui Qu Fa Ke Ti Dai Chuan Tong Rong Ji Ti Qu Fa
Journal of Zhejiang University SCIENCE B