Plasma Markers of Oxidative Stress in Patients with Gestational Diabetes Mellitus in the Second and Third Trimester
Objective. To determine plasma markers of oxidative stress during the second and third trimester of pregnancy in patients with gestational diabetes mellitus (GDM) Study Design. We conducted a prospective nested case control study involving 400 pregnant women, 22 of whom developed GDM. As control group, 30 normal pregnant women were chosen randomly. Plasma samples were analyzed for 8 iso prostaglandin F2a (8 iso PGF2a) advanced oxidative protein products (AOPPs) protein carbonyl (PCO) glutathione peroxidase 3 (GPX 3) and paraoxonase 1 (PON1) at 16 20 weeks, 24 28 weeks, and 32 36 weeks of gestation. Results. Compared to control subjects, the plasma levels of PCO, AOPPs, and 8 iso PGF2a were elevated at 16 20 weeks' and 32 36 weeks' gestation in GDM. There was no significant difference in PCO and 8 iso PGF2a at 24 28 weeks in GDM. GPX 3 was statistically significantly increased at 16 20 weeks and 32 36 weeks in GDM. PON1 reduced in patients with GDM. No significant differences were found at 24 28 and 32 36 weeks between the GDM and control groups. In GDM, PCO, AOPPs, and 8 iso PGF2a levels were higher and GPX 3 and PON1 levels were lower in the second than the third trimester. Conclusion. Oxidation status increased in GDM, especially protein oxidation, which may contribute to the pathogenesis of GDM.
Obstetrics and gynecology international