Dexmedetomidine protects against lung ischemia reperfusion injury by the PI3K/Akt/HIF 1a signaling pathway
PurposeTo evaluate the role of the phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/hypoxia inducible factor 1a (HIF 1a) signaling pathway in the protection by dexmedetomidine against lung ischemia reperfusion injury (IRI) in rats.MethodsForty eight male Sprague Dawley rats weighing 250 350 g were randomly divided into six groups (n 8 each group) sham group, IRI group, low dose dexmedetomidine group (LD group) high dose dexmedetomidine group (HD group) combined low dose dexmedetomidine and LY294002 group (LDL group) and combined high dose dexmedetomidine and LY294002 group (HDL group) A 30 min ischemia was induced by occluding the hilum of the left lung, followed by a 120 min reperfusion by removing occlusion of the hilum. After the left lung was removed, the wet/dry weight ratio (W/D) of the lung tissues was determined. Pathological changes of lung tissues were evaluated by light and electron microscopes and the expression of p Akt and HIF 1a in the lung tissues was determined by western blotting.ResultsCompared with the sham group, both the W/D ratio and lung injury were significantly increased, the p Akt expression was down regulated and HIF 1a expression was up regulated in the five experimental groups. Compared with the LD and LDL groups, both the W/D ratio and lung injury were decreased, but the expression of p Akt and HIF 1a was increased in the HD and HDL groups.ConclusionsAdministration of dexmedetomidine before ischemia can provide a protection against lung IRI by re installing the PI3K/Akt/HIF 1a signaling pathway.
Journal of Anesthesia