Cytotoxic effects of zearalenone and its metabolites and antioxidant cell defense in CHO K1 cells.
Zearalenone (ZEA) and its metabolites (a zearalenol; a ZOL, b zearalenol; b ZOL) are secondary metabolites of Fusarium fungi that produce cell injury. The present study explores mycotoxin induced cell damage and cellular protection mechanisms in CHO K1 cells. Cytotoxicity has been determined by reactive oxygen species (ROS) production and DNA damage. ROS production was determined using the fluorescein assay and DNA strand breakage by comet assay. Intracellular protection systems were glutathione (GSH) glutathione peroxidase (GPx) catalase (CAT) and superoxide dismutase (SOD) The results demonstrated that all mycotoxins increased the ROS levels up to 5.3 fold the control levels in CHO K1 cells. Zearalenone metabolites, but not ZEA, increased DNA damage 43% (a ZOL) and 28% (b ZOL) compared to control cells. The GSH levels decreased from 18% to 36% The GPx and SOD activities respectively increased from 26% to 62% and from 23% to 69% in CHO K1 cells, whereas CAT activity decreased from 14% to 52% In addition, intracellular ROS production was induced by ZEA and its metabolites. The endogenous antioxidant system components GSH, GPx and SOD were activated against ZEA and its metabolites. These antioxidant system components thus could contribute to decrease cell injury by ZEA and its metabolites.
Food and chemical toxicology an international journal published for the British Industrial Biological Research Association