Characterization, Anti Inflammatory and Antiproliferative Activities of Natural and Sulfonated Exo Polysaccharides from Streptococcus thermophilus ASCC 1275.
Exo polysaccharides (EPS) isolated from Streptococcus thermophilus ASCC 1275 were sulfated (31% High performance liquid chromatography identified that EPS was composed of mannose (30.19% galactose (20.10% glucose (18.05% glucosamine (16.04% galactosamine (9.06% glucuronic acid (3.55% and ribose (3.01% Pro /anti inflammatory cytokine secretion ratios (IL 1b/IL 10, IL 6/IL 10, and TNF a/IL 10) of lipopolysaccharide stimulated RAW 264.7 macrophages were significantly decreased by EPS and S.EPS treatments in a dose dependent manner. Furthermore, anti inflammatory activities of S.EPS improved 49.3% and 24.0% than those of EPS before or after LPS treatment. The reactive oxygen species were inhibited by EPS and S.EPS by 49.6% and 55.1% at 50 mg/mL, respectively. Inhibition activities of S.EPS on nitric oxide production were 12.9% and 55.4% higher than those of EPS at 10 and 50 mg/mL. Additionally, S.EPS exhibited stronger antiproliferative activity on Caco 2 and HepG2 cells. Our results indicated that anti inflammatory and antiproliferative activities of EPS were significantly (P 0.01) improved by sulfonation.
Journal of food science