DNAzyme Based Logic Gate Mediated DNA Self Assembly.
Controlling DNA self assembly processes using rationally designed logic gates is a major goal of DNA based nanotechnology and programming. Such controls could facilitate the hierarchical engineering of complex nanopatterns responding to various molecular triggers or inputs. Here, we demonstrate the use of a series of DNAzyme based logic gates to control DNA tile self assembly onto a prescribed DNA origami frame. Logic systems such as "YES," "OR," "AND," and "logic switch" are implemented based on DNAzyme mediated tile recognition with the DNA origami frame. DNAzyme is designed to play two roles: (1) as an intermediate messenger to motivate downstream reactions and (2) as a final trigger to report fluorescent signals, enabling information relay between the DNA origami framed tile assembly and fluorescent signaling. The results of this study demonstrate the plausibility of DNAzyme mediated hierarchical self assembly and provide new tools for generating dynamic and responsive self assembly systems.
Nano letters