A time dependent degeneration manner of condyle in rat CFA induced inflamed TMJ.
Temporomandibular joint (TMJ) inflammation is a potential risk factor of osteoarthritis (OA) but the detailed degenerative changes in the inflamed TMJ remain unclear. In this study, we evaluated the changes of condylar cartilage and subchondral bone in rat inflamed TMJ induced by Freund's complete adjuvant (CFA) Articular cavity was injected with CFA and the TMJ samples were collected 1, 2, 3, and 4 week post injection. Hematoxylin Eosin (H&E) staining, toluidine blue (TB) staining, Safranin O (S.O) staining, Masson trichrome staining and micro CT were used to assess TMJ degeneration during inflammation. Osteoclast and osteoblast activities were analyzed by tartrate resistant acid phosphatase (TRAP) staining and osteocalcin (OCN) immunohistochemistry staining respectively. The expression of receptor activator of NF kB ligand (RANKL) and osteoprotegerin (OPG) in condylar cartilage and subchondral bone was also evaluated through immunohistochemistry and RANKL/OPG ratio was evaluated. Reduced cartilage thickness, decreased number of chondrocytes, and down regulated proteoglycan expression were observed in the condylar cartilage in the inflamed TMJ. Enhanced osteoclast activity, and expanded bone marrow cavity were reached the peak in the 2 week after CFA injection. Meanwhile the RANKL/OPG ratio in the cartilage and subchondral bone also increased in the 2 week CFA injection. Immature, unmineralized new bones with irregular trabecular bone structure, atypical condylar shape, up regulated OCN expression, and decreased bone mineral density (BMD) were found in the inflamed TMJ. The time dependent degeneration manner of TMJ cartilage and subchondral bone was found in CFA induced arthritis rat model. The degeneration in the TMJ with inflammation might be a risk factor and should be concerned.
American journal of translational research