Enhanced overexpression of hypoxia related miRNA 210 in primary tumor of metastatic prostate cancer.
166 Background: miR 210 is a known transcriptional target of the hypoxia responsive HIF 1a signaling pathway. However, the association between the expression of miR 210 and the tumor progression in prostate cancer (PCa) remains unclear. Methods: We isolated miRNA by miRNeasy FFPE kit (Qiagen, Hilden, Germany) from paraffin embedded tissues of 87 prostate specimens with adenocarcinoma of the prostate cancer in different tumor stages, with high grade prostatic intraepithelial neoplasia (HGPIN) and normal tissues. Organ confined PCa was defined as PCa with pT2 tumor stage, Gleason score 7a and PSA level 7a was defined as advanced PCa. PCa with pN1 were considered as metastatic diseases. Additionally, 3 cases with castration resistant prostate cancer (CRPC) were considered. Quantitative miR 210 expression data were acquired and analyzed using a real time TaqMan based PCR with the ABI Prism 7900HT (Life Technologies, Darmstadt, Germany) ANOVA and post hoc analysis acc.