HiChIP: efficient and sensitive analysis of protein directed genome architecture
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein centric chromatin conformation method. HiChIP improves the yield of conformation informative reads by over 10 fold and lowers the input requirement over 100 fold relative to that of ChIA PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal to background ratios than those of in situ Hi C.
Nature Methods