Pre immune state induced by chicken interferon gamma inhibits the replication of H1N1 human and H9N2 avian influenza viruses in chicken embryo fibroblasts
BackgroundInterferon gamma (IFN g) an immunoregulatory cytokine, is known to control many microbial infections. In a previous study, chicken interferon gamma (chIFN g) was found to be up regulated following avian influenza virus (AIV) infection in specific pathogen free chickens. We aimed to investigate whether the pre immune state induced by chIFN g could generate an antiviral response against influenza virus.MethodsWe generated a chIFN g expressing plasmid and transfected it into chicken embryo fibroblasts (CEFs) and then infected the cells with human origin H1N1 or avian origin H9N2 influenza viruses. Viral titers of culture medium were evaluated in MDCK cell and the viral RNA and IFN stimulated genes (ISGs) were then quantified by real time reverse transcriptase polymerase. To further evaluate the role of the antiviral effect of chIFN g by using a backward approach, synthetic small interfering RNAs (siRNA) targeting chIFN g were used to suppress chIFN g.ResultsThe chIFN g stimulated CEFs inhibited the replication of viral RNA (vRNA) and showed a mild decrease in the infectious virus load released in the culture medium. Compared to the mock transfected control, the messenger RNA (mRNA) levels of type I IFNs and IFN stimulated genes were up regulated in the cells expressing chIFN g. After treatment with the siRNA, we detected a higher expression of viral genes than that observed in the mock transfected control.ConclusionsOur results suggest that apart from the important role played by chIFN g in the antiviral state generated against influenza virus infection, the pre immune state induced by chIFN g can be helpful in mitigating the propagation of influenza virus.
Virology Journal