Baseline frequency of chromosomal aberrations and sister chromatid exchanges in peripheral blood lymphocytes of healthy individuals living in Turin (North Western Italy) assessment of the effects of age, sex and GSTs gene polymorphisms on the levels of genomic damage
Abstract Background: The increased exposure to environmental pollutants has led to the awareness of the necessity for constant monitoring of human populations, especially those living in urban areas. Aim: This study evaluated the background levels of genomic damage in a sample of healthy subjects living in the urban area of Turin (Italy) The association between DNA damage with age, sex and GSTs polymorphisms was assessed. Subjects and methods: One hundred and one individuals were randomly sampled. Sister Chromatid Exchanges (SCEs) and Chromosomal Aberrations (CAs) assays, as well as genotyping of GSTT1 and GSTM1 genes, were performed. Results: Mean values of SCEs and CAs were 5.137 0.166 and 0.018 0.002, respectively. Results showed age and gender associated with higher frequencies of these two cytogenetic markers. The eldest subjects (51 65 years) showed significantly higher levels of genomic damage than younger individuals. GSTs polymorphisms did not appear to significantly influence the frequencies of either markers. Conclusion: The CAs background frequency observed in this study is one of the highest reported among European populations. Turin is one of the most polluted cities in Europe in terms of air fine PM10 and ozone and the clastogenic potential of these pollutants may explain the high frequencies of chromosomal rearrangements reported here.
Annals of human biology