Constructing a linkage linkage disequilibrium map using dominant segregating markers
The relationship between linkage disequilibrium (LD) and recombination fraction can be used to infer the pattern of genetic variation and evolutionary process in humans and other systems. We described a computational framework to construct a linkage LD map from commonly used biallelic, single nucleotide polymorphism (SNP) markers for outcrossing plants by which the decline of LD is visualized with genetic distance. The framework was derived from an open pollinated (OP) design composed of plants randomly sampled from a natural population and seeds from each sampled plant, enabling simultaneous estimation of the LD in the natural population and recombination fraction due to allelic co segregation during meiosis. We modified the framework to infer evolutionary pasts of natural populations using those marker types that are segregating in a dominant manner, given their role in creating and maintaining population genetic diversity. A sophisticated two level EM algorithm was implemented to estimate and retrieve the missing information of segregation characterized by dominant segregating markers such as single methylation polymorphisms. The model was applied to study the relationship between linkage and LD for a non model outcrossing species, a gymnosperm species, Torreya grandis, naturally distributed in mountains of the southeastern China. The linkage LD map constructed from various types of molecular markers opens a powerful gateway for studying the history of plant evolution.
DNA research an international journal for rapid publication of reports on genes and genomes