Synthetic Method for Oligonucleotide Block by Using Alkyl Chain Soluble Support.
A straightforward method for the synthesis of oligonucleotide blocks using a Cbz type alkyl chain soluble support (Z ACSS) attached to the 3' OH group of 3' terminal nucleosides was developed. The Z ACSS allowed for the preparation of fully protected deoxyribo and ribo oligonucleotides without chromatographic purification and released dimer to tetramer size oligonucleotide blocks via hydrogenation using a Pd/C catalyst without significant loss or migration of protective groups such as 5' end 4,4' dimethoxtrityl, 2 cyanoethyl on internucleotide bonds, or 2' TBS.
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