Dietary Flavonoid Hyperoside Induces Apoptosis of Activated Human LX 2 Hepatic Stellate Cell by Suppressing Canonical NF kB Signaling
Hyperoside, an active compound found in plants of the genera Hypericum and Crataegus, is reported to exhibit antioxidant, anticancer, and anti inflammatory activities. Induction of hepatic stellate cell (HSC) apoptosis is recognized as a promising strategy for attenuation of hepatic fibrosis. In this study, we investigated whether hyperoside treatment can exert antifibrotic effects in human LX 2 hepatic stellate cells. We found that hyperoside induced apoptosis in LX 2 cells and decreased levels of a smooth muscle actin (a SMA) type I collagen, and intracellular reactive oxygen species (ROS) Remarkably, hyperoside also inhibited the DNA binding activity of the transcription factor NF kB and altered expression levels of NF kB regulated genes related to apoptosis, including proapoptotic genes Bcl Xs, DR4, Fas, and FasL and anti apoptotic genes A20, c IAP1, Bcl X L, and RIP1. Our results suggest that hyperoside may have potential as a therapeutic agent for the treatment of liver fibrosis.
BioMed research international