Antiretroviral therapy induces a rapid increase in bone resorption that is positively associated with the magnitude of immune reconstitution in HIV infection
Objective:Antiretroviral therapy (ART) paradoxically intensifies bone loss in the setting of HIV infection. Although the extent of bone loss varies, it occurs with virtually all ART types, suggesting a common pathway that may be aligned with HIV disease reversal. Using an animal model of immunodeficiency we recently demonstrated that immune activation associated with CD4+ T cell reconstitution induces increased production of the osteoclastogenic cytokines RANKL and TNF&agr; by immune cells, driving enhanced bone resorption and loss in bone mineral density. Design:To confirm these findings in humans, we investigated the early kinetics of CD4+ T cell recovery in relation to biomarkers of bone turnover and osteoclastogenic regulators in a prospective 24 week cohort study. Methods:Clinical data and blood sampling for HIV RNA PCR, CD4+ T cell counts, bone turnover biomarkers, and osteoclastogenic regulators were obtained from ART naive HIV infected study participants initiating standard doses of lopinavir/ritonavir plus tenofovir disoproxil fumarate/emtricitabine at baseline and at weeks 2, 8, 12, and 24 post ART. Results:C terminal telopeptide of collagen (CTx) a sensitive biomarker of bone resorption rose by 200% above baseline at week 12, remaining elevated through week 24 &agr;<0.01) and was associated with significant increases in plasma levels of osteoclastogenic regulators [receptor activator of NF kB ligand (RANKL) tumor necrosis factor alpha, (TNF&agr; Importantly, the magnitude of CD4+ T cell recovery correlated significantly with CTx (rs 0.387, &agr;=0.01) Conclusion:Our data suggest that ART induced bone loss occurs early, is aligned with early events of immune reconstitution, and these immune changes provide a unifying mechanism to explain in part the skeletal decline common to all ART.
AIDS