Secondary metabolites from an infusion of Lippia gracilis Schauer using the LC DAD SPE/NMR hyphenation technique
The genus Lippia comprises approximately 200 species of herbs, shrubs and small trees, distributed throughout the South and Central America and tropical Africa. The species Lippia gracilis Schauer is an endemic aromatic plant of the Brazilian Northeast normally found in the states of Bahia, Sergipe and Piaui. The traditional communities of Caatinga, a semi arid region of the Brazilian Northeast, use its leaves to treat throat and mouth infections, cutaneous diseases, ulcers, vagina disorders, acne, Pityriasis alba, dandruff, burns, wounds, sinusitis, bronchitis, nasal congestion, headache, jaundice and paralysis. Considering that the main investigations into the L. gracilis Schauer species are focused on its volatile constituents, in this report, we describe the isolation, by liquid chromatography with diode array detector solid phase extraction/nuclear magnetic resonance (LC DAD SPE/NMR) of five flavonoids besides free and glycosidically bound carvacrol from an infusion of the leaves of a genotype of this species.