The effect of copper nanoparticles supplementation on freshwater prawn Macrobrachium rosenbergii post larvae.
The present study was performed to assess the effects of dietary supplementation of copper nanoparticles (Cu NPs) on growth, biochemical constituents, digestive enzyme activities, antioxidant, metabolic enzyme levels, and non specific immune response of the freshwater prawn, Macrobrachium rosenbergii post larvae (PL) The Cu NPs (200 nm) were synthesized by facile and environmental friendly hydrothermal method. Cu NPs were supplemented at 0, 10, 20, 40, 60, and 80 mg kg( 1) with the basal diets. These Cu NPs supplemented diets were fed to M. rosenbergii PL for 90 days. Results showed significant (P<0.05) improvements were observed in survival, growth, digestive enzyme activities, concentrations of biochemical constituents and total and differential haemocytes count of prawns fed with 20 mg Cu NPs kg( 1) supplemented feed. Prawns fed with 40 80 mg Cu NPs kg( 1) supplemented feed showed negative performance. Activity of antioxidants and metabolic enzymes in the muscle and hepatopancreas of prawns showed no significant alterations (P>0.05) prawns fed with up to 20 mg Cu NPs kg( 1) supplemented feeds. Whereas, prawns fed with 40 80 mg Cu NPs kg( 1) supplemented feed showed significant (P<0.05) elevations in antioxidant and metabolic enzymes activities. Hence, 40 80 mg Cu NPs kg( 1) diets may have toxic effect to M. rosenbergii. Hence, present study suggests that 20 mg Cu NPs kg( 1) can be supplemented for regulating better survival, growth and immune response of M. rosenbergii PL.
Journal of trace elements in medicine and biology organ of the Society for Minerals and Trace Elements