Development of sensor surfaces using poly (N vinylformamide) for sensitive detection of 2,4,6 trinitrotoluene by displacement method on a surface plasmon resonance sensor
To detect 2,4,6 trinitrotoluene (TNT) using a displacement method over a surface plasmon resonance immunosensor, we developed sensor surfaces modified with a poly (N vinylformamideco vinylamine) which was synthesized from a monomer, N vinylformamide, and was hydrolyzed to produce amine groups, and TNT analogs. Poly (N vinylformamide co vinylamine) that reacted with succinic acid was used as a matrix of a sensor surface for increasing the number of antibody binding sites. 2,4 Dinitrophenyl glycine, 3 nitrophenyl acetic acid, and 4 nitrophenyl acetic acid were immobilized on the polymer modified sensor surfaces. We evaluated the suppression ability of the sensor surface for nonspecific adsorption and determined the limit of detection (LOD) for TNT. As a result, a sensor chip suppressed nonspecific adsorption for 1 mg/mL bovine serum albumin and lysozyme. TNT was measured by the displacement method, and the LOD achieved over a 3 nitrophenyl acetic acid modified sensor chip was 0.9 ng/mL (ppb)