Sophoraflavanone G from Sophora alopecuroides inhibits lipopolysaccharide induced inflammation in RAW264.7 cells by targeting PI3K/Akt, JAK/STAT and Nrf2/HO 1 pathways.
Sophoraflavanone G (SG) a prenylated flavonoid from Sophora alopecuroides, has been reported to have many pharmacological activities including anti inflammation. However, the molecular mechanisms of its anti inflammatory activity remain largely unclear. In this study we investigated the effects and the underlying molecular mechanisms of SG on lipopolysaccharide (LPS) induced inflammation in RAW264.7 cells. Pretreatment with SG inhibited LPS induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) through reducing the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX 2) SG also decreased the expressions of pro inflammatory cytokines, tumor necrosis factor a (TNF a) interleukin 6 (IL 6) and interleukin 1b (IL 1b) both in the protein and gene levels. Further experiments demonstrated that SG downregulated the LPS induced upregulation of phosphorylated phosphoinositide 3 kinase and Akt (PI3K/Akt) SG also attenuated the expression of phosphorylated Janus kinase signal transducer and activator of transcription (JAK/STAT) In addition, SG upregulated heme oxygenase 1 (HO 1) expression via nuclear translocation of nuclear factor E2 related factor 2 (Nrf2) Taken together, SG may act as a natural agent to treat some inflammatory diseases by targeting PI3K/Akt, JAK/STAT and Nrf2/HO 1 pathways.
International immunopharmacology