C2c2 is a single component programmable RNA guided RNA targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly interspaced short palindromic repeat (CRISPR) CRISPR associated genes (Cas) adaptive immune systems, which protect microbes against viruses and other foreign DNA. All functionally characterized CRISPR systems have been reported to target DNA, with some multicomponent type III systems also targeting RNA. The putative class 2 type VI system, which has not been functionally characterized, encompasses the single effector protein C2c2, which contains two Higher Eukaryotes and Prokaryotes Nucleotide binding (HEPN) domains commonly associated with ribonucleases (RNases) suggesting RNA guided RNA targeting function. RATIONALE Existing studies have only established a role for RNA interference, in addition to DNA interference, in the multicomponent type III A and III B systems. We investigated the possibility of C2c2 mediated RNA inference by heterologously expressing C2c2 locus from Leptotrichia shahii (LshC2c2) in the model system Escherichia coli. The ability of LshC2c2 to protect against MS2 single stranded RNA (ssRNA) phage infection was assessed by using every possible spacer sequence against the phage genome. We next developed protocols to reconstitute purified recombinant LshC2c2 protein and test its biochemical activity when incubated with its mature CRISPR RNA (crRNA) and target ssRNA. We systematically evaluated the parameters necessary for cleavage. Last, to demonstrate the potential utility of the LshC2c2 complex for RNA targeting in living bacterial cells, we guided it to knockdown red fluorescent protein (RFP) mRNA in vivo. RESULTS This work demonstrates the RNA guided RNase activity of the putative type VI CRISPR effector LshC2c2. Heterologously expressed C2c2 can protect E. coli from MS2 phage, and by screening against the MS2 genome, we identified a H (non G) protospacer flanking site (PFS) following the RNA target site, which was confirmed by targeting a complementary sequence in the b lactamase transcript followed by a degenerate nucleotide sequence. Using purified LshC2c2 protein, we demonstrate that C2c2 and crRNA are sufficient in vitro to achieve RNA guided, PFS dependent RNA cleavage. This cleavage preferentially occurs at uracil residues in ssRNA regions and depends on conserved catalytic residues in the two HEPN domains. Mutation of these residues yields a catalytically inactive RNA binding protein. The secondary structure of the crRNA direct repeat (DR) stem is required for LshC2c2 activity, and mutations in the 3' region of the DR eliminate cleavage activity. Targeting is also sensitive to multiple or consecutive mismatches in the spacer:protospacer duplex. C2c2 targeting of RFP mRNA in vivo results in reduced fluorescence. The knockdown of the RFP mRNA by C2c2 slowed E. coli growth, and in agreement with this finding, in vitro cleavage of the target RNA results in "collateral," nonspecific cleavage of other RNAs present in the reaction mix. CONCLUSION LshC2c2 is a RNA guided RNase which requires the activity of its two HEPN domains, suggesting previously unidentified mechanisms of RNA targeting and degradation by CRISPR systems. Promiscuous RNase activity of C2c2 after activation by the target slows bacterial growth and suggests that C2c2 could protect bacteria from virus spread via programmed cell death and dormancy induction. A single effector RNA targeting system has the potential to serve as a general chassis for molecular tools for visualizing, degrading, or binding RNA in a programmable, multiplexed fashion. C2c2 is an RNA guided RNase that provides protection against RNA phage. CRISPR C2c2 from L. shahii can be reconstituted in E. coli to mediate RNA guided interference of the RNA phage MS2. Biochemical characterization of C2c2 reveals crRNA guided RNA cleavage facilitated by the two HEPN nuclease domains. Binding of the target RNA by C2c2 crRNA also activates a nonspecific RNase activity, which may lead to promiscuous cleavage of RNAs without complementarity to the crRNA guide sequence. The clustered regularly interspaced short palindromic repeat (CRISPR) CRISPR associated genes (Cas) adaptive immune system defends microbes against foreign genetic elements via DNA or RNA DNA interference. We characterize the class 2 type VI CRISPR Cas effector C2c2 and demonstrate its RNA guided ribonuclease function. C2c2 from the bacterium Leptotrichia shahii provides interference against RNA phage. In vitro biochemical analysis shows that C2c2 is guided by a single CRISPR RNA and can be programmed to cleave single stranded RNA targets carrying complementary protospacers. In bacteria, C2c2 can be programmed to knock down specific mRNAs. Cleavage is mediated by catalytic residues in the two conserved Higher Eukaryotes and Prokaryotes Nucleotide binding (HEPN) domains, mutations of which generate catalytically inactive RNA binding proteins. These results broaden our understanding of CRISPR Cas systems and suggest that C2c2 can be used to develop new RNA targeting tools.
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