Enzymatic Resolution by a d Lactate Oxidase Catalyzed Reaction for (S) 2 Hydroxycarboxylic Acids
Oxidase catalyzed kinetic resolution is important for the production of enantiopure 2 hydroxycarboxylic acids (2 HAs) which are versatile building blocks for the synthesis of many significant compounds. However, in contrast to that of (R) 2 HAs, the production of (S) 2 HA is challenging because of the lack of related oxidases. Herein, suitable enzymes were screened systematically through the analysis of numerous putative d lactate oxidase sequences and identification of several required properties. Finally, a d lactate oxidase from Gluconobacter oxydans 621H with advantageous characteristics, such as good solubility, broad substrate spectrum, and high stereoselectivity, was selected to resolve 2 HAs into (S) 2 HAs. A variety of (S) 2 HAs was produced successfully using this d lactate oxidase with excellent enantiomeric excess values >99 The presented screening criteria and approach for target biocatalysis suggested a guideline for the production of optically active chemicals such as (S) 2 HAs.