Significance of EMSA Eritin Administration on Erythropoiesis and Complement Regulators in Irradiated Mice
OZ Amac: Bu calismada Balb/c farelerde 500 rad doz ile total vucut irradyasyonu (TVI) sonrasi poliherbal eritropoez modulator ve stimulator ajan (EMSA) Eritin uygulamasinin TER 119+VLA 4+ hucrelerine gore eritropoez aktivitesi ve TER 119+CD55+ ile TER 119+CD59+ hucrelerine gore kompleman regulatorleri modulasyonu uzerindeki etkisi incelendi. Gerec ve yontemler: Dusuk doz (1.04 mg/g BW) normal doz (3.125 mg/g BW) ve yuksek doz (9.375 mg/g BW) icerecek sekilde iki hafta boyunca gunde 500 rad dozunda TVI'ye maruz kalan farelere oral yoldan EMSA Eritin uygulandi. Fareler pozitif kontrol olarak haftada iki kere 0.21 mg/g BW dozunda HEMAPO Epoetin alfa ile tedavi edildi. On besinci gunde kemik iligi izole edildi ve akis sitometrisi kullanilarak TER 119+VLA 4+ TER 119+CD55+ ve TER 119+CD59+ hucrelerin sayisi analiz edildi. Inceleme, 500 rad dozunda TVI sonrasi farelerin kemik iligindeki TER 119+VLA 4+ hucrelerin sayisinda saglikli kontrollere gore anlamli azalma oldugunu gosterdi. Radyasyona maruz kalan farelerde EMSA Eritin ile TER 119+VLA 4+ hucrelerin sayisi TVI olan fareler ve Epoetin alfa ile tedavi edilen farelere gore dusuk dozdan yuksek doza anlamli sekilde artti. Bulgular: En yuksek dozda EMSA Eritin eritropoezi desteklemede HEMAPO Epoetin alfa ve diger dozdaki EMSA Eritin'e gore daha etkili idi. Sonuc: Calisma bulgularimiza gore, EMSA Eritin 500 rad doz ile irradyasyon sonrasi homeostaz ve eritropoezi normallestiren koruyucu bir ajan islevi gosterebilecek guclu bir tedavici edici herbal destekleyicidir. Anahtar sozcukler: Antioksidan; kemik iligi; kompleman regulatorler; eritropoez; total vucut irradyasyonu. ABSTRACT Objectives: This study aims to examine the effect of polyherbal erythropoiesis modulatory and stimulatory agent (EMSA) Eritin administration in modulating the activity of erythropoiesis on TER119+VLA 4+ cells and complement regulators TER 119+CD55+ and TER 119+CD59+ cells in Balb/c mice after total body irradiation (TBI) with a dose of 500 rad. Materials and methods: EMSA Eritin was administered orally in mice after exposure to TBI with a dose of 500 rad daily for two weeks with three different doses including a low dose (1.04 mg/g BW) normal dose (3.125 mg/g BW) and high dose (9.375 mg/g BW) Mice were treated with HEMAPO Epoetin alfa twice a week as positive control with a dose of 0.21 mg/g BW. On day 15, the bone marrow was isolated and the number of TER 119+VLA 4+ TER 119+CD55+ and TER 119+CD59+ cells were analyzed using flow cytometry. Examination showed marked reduction in the number of TER 119+VLA 4+ cells in the bone marrow of mice after TBI with dose of 500 rad compared to those of healthy controls. The number of TER 119+VLA 4+ cells in the irradiated mice was markedly increased with EMSA Eritin from low dose to high dose compared to mice with TBI and mice treated with Epoetin alfa. Results: The highest dose of EMSA Eritin was more effective to promote erythropoiesis than HEMAPO Epoetin alfa and the other dose of EMSA Eritin. Conclusion: Our study findings suggest that EMSA Eritin is a powerful medicinal herbal supplement which may serve as a protective agent to normalize homeostasis and erythropoiesis after irradiation with a dose of 500 rad.