Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter.
Diverse exopolysaccharide (EPS) producing isolates were isolated from an Indian acidic fermented food (Idli) based on the colony morphology. One of the EPS producing microflora (Leuconostoc lactis KC117496) was selected for further characterization using FT IR, HPTLC, AFM, SEM, TGA and XRD analysis. FT IR spectroscopy revealed the a d glucose nature of the EPS. HPTLC analysis confirmed the presence of only glucose monomers, indicating the glucan nature of EPS. NMR spectra revealed the presence of 95% a (1 6) and 5% branching a (1 3) linkages. The SEM and AFM showed smooth surfaces and compact structure. TGA results showed higher degradation temperature of 272.01degC. XRD analysis proved the 33.4% crystalline nature of the EPS. Water solubility index and water holding capacity of EPS are 14.2+ 0.208% and 117+ 7.5% All the above characteristics of the EPS produced by L. lactis showed that the EPS is of a good quality polysaccharide with potential applications in the food industry.
International journal of biological macromolecules