Influence of doxorubicin on apoptosis and oxidative stress in breast cancer cell lines.
Breast cancer is one of the leading causes of mortality among women worldwide due to aggressive behavior, early metastasis, resistance to existing chemotherapeutic agent and high mortality rate. Doxorubicin (Dox) is a powerful antitumoral drug. It is one of the most active agents for treatment of breast cancer. The aim of the present study was to evaluate the influence of Dox in apoptosis and oxidative stress in the breast cancer cell lines MCF 10F, MCF 7 and MDA MB 231. These studies showed that Dox decreased anti apoptotic Bcl 2 protein expression and affected oxidative stress by increasing hydrogen peroxide production and simultaneously decreasing NF kB gene and protein expression in MCF 7, a tumorigenic triple positive cell line. Results also indicated that Dox induced apoptosis by upregulating Bax, caspase 8 and caspase 3 and downregulation of Bcl 2 protein expression. On the contrary, ROS damage decreased by increasing SOD2 gene and protein expression and hydrogen peroxide production with parallel NF kB protein expression decrease in MDA MB 231, a tumorigenic triple negative breast cancer cell line. It can be concluded that Dox activated apoptosis by inducing proteolytic processing of Bcl 2 family, caspases and simultaneously decreased oxidative stress by influencing ROS damage in MCF 7 and MDA MB 231 cell lines.
International journal of oncology