Use of FGF 2 and FGF 18 to direct bone marrow stromal stem cells to chondrogenic and osteogenic lineages
Aim: Intervertebral disc degeneration/low back pain is the number one global musculoskeletal condition in terms of disability and socioeconomic impact. Materials methods Multipotent mesenchymal stem cells (MSCs) were cultured in micromass pellets FGF 2 or 18 up to 41 days, matrix components were immunolocalized and gene expression monitored by quantitative reverse transcription PCR. Results: Chondrogenesis occurred earlier in FGF 18 than FGF 2 cultures. Lower COL2A1, COL10A1 and ACAN expression by day 41 indicated a downregulation in chondrocyte hypertrophy. MEF2c, ALPL, were upregulated; calcium, decorin and biglycan, and 4C3 and 7D4 chondroitin sulphate sulfation motifs were evident in FGF 18 but not FGF 2 pellets. Conclusion: FGF 2 and 18 preconditioned MSCs produced cell lineages which promoted chondrogenesis and osteogenesis and may be useful in the production of MSC lineages suitable for repair of cartilaginous tissue defects.
Future science OA