Brachiaria piligera and Sorghum verticilliflorum are natural hosts of two different strains of sugarcane mosaic virus in Australia
In 1985 and 1986, during an outbreak of mosaic in sugarcane in the lsis district of Queensland, Brachiaria piligera (hairy arm grass) with mosaic symptoms was found infected with the sugarcane strain of sugarcane mosaic virus (SCMV-SC). This is the first report of SCMV-SC naturally infecting a host other than sugarcane in Australia. Sorghum verticilliflorum (wild sorghum) with mosaic symptoms in the lsis and nearby coastal districts was always infected by Johnsongrass mosaic virus (JGMV), except at one site in Bundaberg where the sabigrass strain of SCMV (SCMV-Sabi), which does not infect sugarcane, was found. This is the first report of SCMV-Sabi occurring in wild sorghum and in a district distant from Gatton where infected sabigrass occurs normally.
Gold-labelled antibody decoration (GLAD) in the diagnosis of plant viruses by immuno-electron microscopy.
Abstract The diagnostic capabilities of immuno-electron microscopy have been developed by using antibodies labelled with colloidal gold-protein A. A method termed gold-labelled antibody decoration (GLAD) is described and results using the technique on a number of strains of tobacco mosaic virus are given.
Staphylococcal protein a bound to colloidal gold: A useful reagent to label antigen-antibody sites in electron microscopy
Abstract Purified staphylococcal protein A (SpA) was combined with colloidal gold. SpA coated with gold particles, gold-labelled SpA, retained the property of combining with teh Fc portion of IgG immunoglobulin. Lymphycytes, platelets, virus-transformed rat kidney cells, vesicular stomatitis virus (VSV) adn red cells were coated withe hte specific antibody and then incubated with the gold-SpA reagent. Using electron microscopy, gold-labelled SpA appeared as very dense dots which specifically labelled the antigen-antibody sites. This method of labelling surface antigens has the following advantages: there is no covalent bond involved between gold particles and SpA, therefore the preparation of the reagent is very simple; there is no need to purify the antibody to the specific antigen, thus cells may be incubated with whole antiserum.