Cost-effective multiplication of the entomopathogenic fungus Nomuraea rileyi (F) Samson
Cost-effective and rapid multiplication of Nomuraea rileyi is reported. The spore yields in semi-synthetic media were comparable or significantly higher to the standard medium. Maltose and peptone, carbon and nitrogen sources could be effectively replaced with 2% barley extract and 1% soybean extract respectively. However, replacement of yeast extract with dry yeast resulted in lower spore yields. Sporulation of the fungus multiplied on solid substrate was possible only when the bags used had a 0.2 μm filter to facilitate passive exchange of sterile air. A high spore yield of 2.8 × 109/g of substrate was realized on crushed sorghum.
Effect of Nutrients and pH on the Growth and Sporulation of Four Entomogenous Hypomycetes Fungi (Deuteromycotina)
Growth of Metarrhizium flavoviride var. minus and Hirsut lle strigosa showed good yield in the carbon source media adding dextrose, starch and saccharose, but Hirsutella sp. from korea greq well in the other media except in the dextrose media. Yeast extract was necessary for the mecelial growth of the fungi, but the fungi tested in this experiment showed a difference in the amount of required yeast extract. Growth of Nomurea rileyi was fastidious in the carbon and nitrorgen sourced media and the optimum pH of the media for growth was at 6.7. Sporulation of M. Flavoviride var. minus was high on media, containing 1%~2% of yeast extract as nitrogen and carbon source media, but N. rileyi sporulated abundantly on the media with nitrogen and dextrose.
Attention and "visual field dependency" in the pigeon.
Three pigeons were trained in an upright conditioning chamber to peck a key transilluminated by a vertical line. This training was followed by a line orientation generalization test. During the test, the chamber was tilted laterally 22.5 degrees from upright. The chamber floor remained horizontal with respect to gravity. Under these conditions, the subjects responded more often in the presence of a visually vertical (parallel to chamber walls) line orientation than in the presence of a gravitationally vertical line orientation. Subsequent reinforcement of pecking in the presence of a line that was always gravitationally vertical but not always visually vertical temporarily abolished this "visual field dependency" and resulted in generalization gradients with peak responding in the presence of the gravitationally vertical line orientation. The results are discussed in terms of selective attention to the gravitational and visual components of line orientation.