Assessment of production and dispersal of inoculum ofAlternaria Macrospora in various parts of the cotton canopy
Morning and evening counts of spores ofAlternaria macrospora in a cotton (Gossypium barbadense) field were assessed for the number of spores (a) produced on the last night, (b) dispersed during the day, and (c) retained in the spore reserve on leaves. Throughout the season, 1 m2 of the field produced (P) and dispersed (D) approximately 163 x 106 and 137 x 106 spores, respectively. The 16% discrepancy between P and D values reflects experimental errors. Most spores were produced on shed leaves on the ground, fewer on leaves in the lower part of the canopy, and least on leaves in the upper canopy. The contribution to total D decreased from leaves in the lower part of the canopy, to shed leaves, and to leaves in the upper canopy. In tests with potted plants with spore-bearing lesions attached to various heights of the canopy under various conditions of weather, dispersal of spores at these heights was conditioned mainly by wind velocity, which decreased from the upper to the lower part of the canopy. In leaves close to the ground, approximately 29% of the released spores were removed by sedimentation to the ground. On any one day, the daily rate of D differed from the daily rate of P. On days in which D was higher than P, the difference was due to a reserve of spores remaining on lesions after dispersal during the previous days. In weather adverse for production of new spores, the reserve was depleted within 4 and 6 days in the top and bottom leaves, respectively, but remained relatively high in the shed leaves on the ground; a similar number of days was needed to restore the depleted reserve in sporulation-favorable weather.
The infection process, sporulation and survival of Alternaria helianthi on sunflower
SUMMARY 
 
Electron microscopy was used to study the infection of sunflower leaves by Alternaria helianthi. Conidia germinated by producing one to many germ tubes which grew across the leaf surface before forming appressoria. The fungus directly penetrated its host through the cuticle and epidermis. Entry into the host through wounds and stomates was also observed. Extracellular sheaths were found to be associated with germ tubes and intercellular hyphae of A. helianthi. Conidiophores developed through collapsed stomates, from leaf veins, trichomes and also from mycelium growing across the host leaf surface. Microcylic conidia were produced directly from parent conidia under certain conditions. Studies using a volumetric spore trap showed that the airborne spore concentration followed a distinct periodicity with peaks occurring between 0900 and 1100 h each day. Laboratory studies showed that safflower, noogoora burr and bathurst burr could serve as alternative hosts for A. helianthi. The pathogen was readily isolated from sunflower crop debris from a diseased crop that had been harvested 1 yr earlier.
Induction of sporulation of Alternaria porri f. sp. Solani by inhibition of its vegetative development
Alternaria porri (Ellis) Cif. f. sp. solani (Ellis & Mart.) Neerg. on potato and tomato plants kept under continuously moist conditions either in dark or in light or in photoperiods covered the leaves with mycelium, but produced no or few spores. Under all light conditions, interrupting the continuously moist period with 1–3 days of dryness resulted in abundant sporulation. In tomatoes, the highest sporulation occurred when the final day of moisture started with a 12-h light period. Formation of secondary spores in vitro was induced by treatments which interfered with normal germination of mother spores. These treatments included application of toxins, deficiency in nutrients, shocks of high and low temperatures, intermittent drying and exposure to high osmotic pressure. It was concluded that sporulation may be induced by various factors which partially inhibit the vegetative development of the fungus.