SiCp/Cu composites prepared by the dual-directional extrusion process of floating die
SiCp/Cu composites were fabricated by the dual-directional extrusion process of the floating die. The microstructure, wear behavior and conductivity of SiCp/Cu composites were studied by optical microscope, abrasion test and current conductivity instrument. The results show that the SiCp/Cu composites with SiC particle distributed uniformly can be obtained, and the conductivity of composites decreased with the increasing of concentration of SiC particles, the wear resistance behavior of SiCp/Cu composites is evidently better than Cu with the increasing of concentration of SiC particles, the wearing mechanism of composites is a synthetically combined function of micro-cutting wearing and surface peeling off and grain wearing.
Study on Thermal Fatigue Properties of Functional Gradient Materials of SiC_p/Cu
Four kinds of functionally gradient materials were fabricated by powder metallurgy.The microstructure,micro hardness and thermal fatigue properties of tested functional gradient materials were studied with SEM,XRD and automatic micro hardness tester.The results showed that the matrix of SiCp/Cu functional gradient material was continuous,the gradient transition layer was uniform,the microstructure and micro hardness changed gradually.It was evident that the more uniform change of the reinforcement particles in gradient transition layer to be the better thermal fatigue properties of SiCp/Cu functionally gradient material is.
A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity.
Natural (or “spontaneous”) cytotoxicity against the K-562 myeloid cell line and antibody-dependent cellular cytotoxicity (ADCC) against sensitized Chang liver cells were monitored simultaneously in a 4 hr 51 Cr release assay, using the same preparations of normal human peripheral blood lymphocytes as effector cells. Both types of cytotoxic activity were expressed by lymphocytes bearing receptors for sheep erythrocytes and for the Fc portion of IgG. Up to 80% of the total natural killer (NK) cell and K cell (ADCC) activities were recovered in E-rosette pellets separated under optimal conditions. Since the ability to form rosettes with sheep erythrocytes is primarily a marker for human T cells, it seems likely that both NK and K cells are in the T cell lineage. Further, both NK and K cell cytolytic activities were severely depressed after brief exposure of lymphocyte suspensions or rosette pellets to ammonium chloride solutions. Both activities returned to normal values after 24-hr incubation at 24°C. Such sensitivity of human lymphocytes to ammonium chloride solutions may explain why other investigators have failed to observe NK or ADCC activity by E-RFC. When lymphocytes bearing Fc receptors were adsorbed onto plastic surfaces coated with a monolayer of immobilized antigen-antibody complexes made up of TNP and rabbit anti-TNP serum, both NK and ADCC activities were simultaneously depleted. Such depletion was completely blocked when Staphylococcal protein A, which binds selectively to the Fc portion of IgG, was incubated on the antigen-antibody monolayers before adding the lymphocytes. This confirmed that both NK and ADCC activities were mediated by effector lymphocytes bearing Fc receptors. When protein A at concentrations ranging from 0.01 to 100 µg/ml was added to the 51 Cr release assays, ADCC was significantly inhibited, presumably due to binding of protein A to available Fc sites of the sensitizing antibody. However, protein A had no effect on NK reactivity at any of the concentrations tested. When lymphocytes were treated for 30 min at 37°C with purified trypsin or α-chymotrypsin at concentrations from 0.01 to 10 mg/ml, NK activity was significantly inhibited whereas ADCC was unaffected. Other enzymes depressed (pronase, subtilisin) had no effect except at high concentrations (lipase), or stimulated (neuraminidase) both NK and K cell activity similarly, and therefore failed to distinguish between the effector cell types. Thus, our data indicate that effector cells in NK and ADCC are in overlapping, if not identical, subpopulations of Fc receptor-bearing lymphocytes of probable T cell lineage. The cytolytic mechanisms in NK and ADCC appear to be distinct, since we have been unable to find evidence for an “arming” antibody involved in this NK system.