New inhibitors of melanogenesis, OH-3984 K1 and K2. II. Physico-chemical properties and structural elucidation.
New melanin synthesis inhibitors, OH-3984 K1 and K2, were isolated from the fermentation broth of Streptomyces sp. OH-3984, and their structures were elucidated by spectroscopic methods and by chemical transformations. OH-3984 K1 (M.W.: 306; C18H26O4) and K2 (M.W.: 308; C18H28O4) have unique gamma-lactone rings, both of which correspond to oxidative products derived from C1-O14 cleavage of the 14-membered lactone group.
New inhibitors of melanogenesis, OH-3984 K1 and K2. I. Taxonomy, fermentation, isolation and biological characteristics.
Melanogenesis inhibitors, OH-3984 K1 and K2 were isolated from fermentation broth of Streptomyces sp. OH-3984. OH-3984 K1 and K2 inhibited the melanogenesis of B16 melanoma cells at concentrations of 7.5 and 3.8 micrograms/ml, respectively, whereas inhibition of tyrosinase activity has not been observed. The microbial metabolites showed no antimicrobiological activities against Gram-positive and Gram-negative bacteria, fungi or yeast at a concentration of 1,000 micrograms/ml.
Ultrastructural observations of the spermiogenesis in the Parotoplaninae (Plathelminthes, Proseriata) with special reference to a striated appendage of the intercentriolar body
SummaryThe spermiogenesis of three species of the Parotoplaninae (Otoplanidae, Proseriata) is described based on electron-microscopical observations. Special reference is given to organelles which do not persist in mature male gametes. One of these organelles is a striated appendage of the intercentriolar body. This differentiation has not been reported from any other plathelminth taxa up to now. The striated appendage, which may serve as a strengthening element, is hypothesized to be an autapomorphic feature of the Parotoplaninae.