Brachiaria piligera and Sorghum verticilliflorum are natural hosts of two different strains of sugarcane mosaic virus in Australia
In 1985 and 1986, during an outbreak of mosaic in sugarcane in the lsis district of Queensland, Brachiaria piligera (hairy arm grass) with mosaic symptoms was found infected with the sugarcane strain of sugarcane mosaic virus (SCMV-SC). This is the first report of SCMV-SC naturally infecting a host other than sugarcane in Australia. Sorghum verticilliflorum (wild sorghum) with mosaic symptoms in the lsis and nearby coastal districts was always infected by Johnsongrass mosaic virus (JGMV), except at one site in Bundaberg where the sabigrass strain of SCMV (SCMV-Sabi), which does not infect sugarcane, was found. This is the first report of SCMV-Sabi occurring in wild sorghum and in a district distant from Gatton where infected sabigrass occurs normally.
Population dynamics and management of vectors of viruses causing maize lethal necrosis disease in Bomet County Kenya
A Thesis submitted in Fulfillment of the Requirements for the Award of the Degree of Doctor of Philosophy in Crop Protection (Agricultural Entomology Option) in the School of Agriculture and Enterprise Development, Kenyatta University April, 2018
Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA).
The suitability of two ELISA procedures for detecting different strains of tobacco mosaic virus (TMV) by means of a single labeled antibody preparation has been determined. The specificity of the double-antibody sandwich method of ELISA was so great that enzyme conjugates prepared with antibodies to one strain did not react with closely related strains. The indirect method of ELISA which uses an antiglobulin enzyme conjugate was capable of detecting a wide range of TMV strains with antiserum to one strain only. The indirect procedure is thus better suited for diagnostic work. Both methods of ELISA were able to detect relationships between the coat proteins of different TMV strains. These results confirm that the dissociated subunits of different strains are more closely related than the corresponding intact virions.