Collection and evaluation of Hedychium species of Manipur, Northeast India
NE India is considered as one of the centre of diversity of gingers having a total account of 24 species out of the 65 valid species found in the world. Manipur is one of the seven states of NE India with a mere area of 0.7% out of the total area of India. This paper focuses on the distribution, classification and evaluation of Hedychium species collected from Manipur. A total of 11 species were collected, identified and classified based on their morphological characteristics. The species are identified as Hedychium rubrum A.S. Rao et Verma, H. coronarium Koenig, H. chrysoleucum Hook., H. coccineum Buch.-Ham., H. thrysiforme Ker-Gawl., H. flavescens Carey ex Roscoe, H. stenopetalum Lodd., H. urophyllum Lodd., H. marginatum Clarke, H. villosum Wall. and H. elatum R. Brown. Evaluation of its botanical characteristics are also conducted. Analytical hierarchy process method is used in this paper to address the possibility of analysing the potential of these diverse Hedychium resources based on their ornamental, adaptability and utilisation potential which was evaluated on 12 indicator points. H. elatum scored the highest overall index making it the most potential species for sustainable exploitation followed by H. coronarium while H. villosum ranked the lowest.
Investigation and evaluation of the genus Lilium resources native to China
China is known as an important distribution center for the genus Lilium, with 55 species having been reported in the country thus far. This study was conducted to provide the first comprehensive evaluation of Lilium distribution in China. A total of 27 species of Lilium and 3 varieties were found to exist in the main centers of Lilium distribution in China; these species were identified and classified based on their morphological characteristics. The distribution and botanical characteristics of the various species are reviewed in this study. We also describe differences, mainly in flower color within the same species, which may be representative of new natural varieties or ecotypes. An analytical hierarchy process (AHP) utilizing 16 indicator characteristics was used to evaluate the collected Lilium resources based on their ornamental value, utilization potential, and ecological adaptability. L. dauricum received the highest AHP scores, indicating that it was the species best suited for sustained exploitation. In addition, L. henryi, L. rosthornii, L. tsingtauense, L. concolor var. pulchellum, L. lancifolium, and species with trumpet–shaped flowers also showed better potential for exploitation than other species. This study also provides references regarding the effective exploitation and use of wild Lilium resources.
Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers
Buffalograss [Buchloe dactyloides (Nutt.) Englem] germplasm has a broad resource of genetic diversity that can be used for turfgrass, forage and conservation. Buffalograss is the only native grass that is presently used as a turfgrass in the Great Plains region of North America. Its low growth habit, drought tolerance and reduced requirement for fertilizer and pesticides contribute to interest in its use. The objectives of this study were to use sequence-related amplified polymorphism (SRAP) markers in the evaluation of genetic diversity and phenetic relationships in a diverse collection of 53 buffalograss germplasms, and to identify buffalograss ploidy levels using flow cytometry. Based on their DNA contents, buffalograss genotypes were grouped into four sets, corresponding to their ploidy levels. Thirty-four SRAP primer combinations were used. This is the first report of the detection of differentiating diploid, tetraploid, pentaploid and hexaploid buffalograss genotypes, representing diverse locations of origin, using SRAP markers. Cluster analysis by the unweighted pair-group method with arithmetic averages based on genetic similarity matrices indicated that there were eight clusters. The coefficients of genetic distance among the genotypes ranged from 0.33 up to 0.99 and averaged D=0.66. The genetic diversity estimate, He, averaged 0.35. These results demonstrated that genotypes with potential traits for turfgrass improvement could readily be distinguished, based on SRAP. The use of PCR-based technologies such as SRAP is an effective tool for estimating genetic diversity, identifying unique genotypes as new sources of alleles for enhancing turf characteristics, and for analyzing the evolutionary and historical development of cultivars at the genomic level in a buffalograss breeding program.