Data of PCL-b-P(MMA-DMAEMA)2 characterization and related assays
The data presented here are related to the research paper entitled “PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors” by Franco et al. [1]. Characterization data of PCL-diol, macroinitiator Br-PCL-Br, homopolymers (PMMA and PDMAEMA) and copolymers (batch 1 and batch 2) analyzed by FTIR, SEC and NMR, as well as, characterization of PCL-NS formulation by laser diffraction and DLS analysis, initial nanocapsule formulations and 1C-NC and 2C-NC formulations, including hydrodynamic diameter at different pH media, and DMSO cytotoxicity.
PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors
Abstract A novel poly(e-caprolactone) (PCL) and poly [(methyl methacrylate)- co -(2-dimethylamino)ethyl methacrylate) 2 ] block copolymer, PCL- b -P(MMA- co- DMAEMA) 2 , was synthesized using atom transfer radical polymerization to obtain pH-sensitive nanocapsules intended for drug delivery to solid tumors. The synthesis was performed using PCL:DMAEMA:MMA at two different molar proportions 0.2:10:1 and 0.7:33:1. Nanocapsules prepared using PCL- b -P(MMA- co- DMAEMA) 2 proved sensitivity to pH, presenting z-average hydrodynamic diameters of 73 and 71 nm at pH 7.4, and 96 and 101 nm at pH 5.5, respectively, because of the decrease in the diffusion coefficient of the nanocapsules due to the increase in amine protonation forming ammonium at the surface. Zeta potential at pH 7.2 was + 23 mV for batch 1 and + 13 mV for batch 2. In vitro cytotoxicity of the materials (solutions) and the nanocapsules (dispersed in water) was evaluated in a tumoral cell model (human breast adenocarcinoma cell line, MCF-7) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT). The homopolymers PDMAEMA and PMMA showed low cytotoxicity up to 0.60 and 0.44 mmol L − 1 , respectively. After applying the nanoformulation, the cell viability was not affected for copolymer concentrations up to 340 mg L − 1 . The results showed the new triblock copolymer is a promising material to be used in novel nanocapsule formulations intended for drug delivery to solid tumors.
PLEASURE, POWER, AND THE PURSUIT OF COMMUNISM: SOVIET YOUTH AND STATE-SPONSORED POPULAR CULTURE DURING THE EARLY COLD WAR, 1945-1968
art, with certain students accused of “showing political immaturity, failing to understand the Party’s positions on art.” This episode constituted part of a broader crackdown on abstract art by the Khrushchev administration from late 1962, precipitated the the Soviet leader’s derision of such art forms in December 1962 at the Manege exhibit. Such instances demonstrate the limitations of the top-level promotion of youth grassroots initiative, as the Khrushchev leadership reigned in youth autonomy when it threatened to go beyond the boundaries of the permissible. The dates cited above have particular resonance, since both 1957-58 and 1962-63 represent two periods when those at the top of the Party hierarchy moved away from pursuing a more pluralistic, tolerant policy course, due to anxiety inspired by both sharpening Cold War hostilities and domestic developments. In these periods, official rhetoric censured what it labeled as 639 TsAOPIM, f. 635, op. 13, d. 546, ll. 56-57. This is likely related to criticism of the Party associated with the fallout from the Secret Speech and the Hungary invasion. For more, see Polly Jones, “From the Secret Speech to the Burial of Stalin: Real and Ideal Responses to De-Stalinization,” in Jones ed., The Dilemmas, 41-63. 640 TsAOPIM, f. 6083, op. 1, d. 52, ll. 166-67. 641 Susan E. Reid, “In the Name of the People: The Manege Affair Revisited,” Kritika 6.4 (Fall 2005): 673716. 642 On these anxieties, see Dobson, Khrushchev’s Cold Summer, 156-88.