Phosphorus Level Affects Brown Blotch Disease, Development and Yield of Cowpea
Application of Phosphorus in adequate concentrations could directly or indirectly reduce disease incidence and severity of cowpea brown blotch and improve the forage and grain yield. A field experiment was conducted to investigate the effects of level of phosphorus application on Brown Blotch disease of cowpea (Vigna unguiculata (L.) Walp), caused by Colletotrichum capsici during 2003, 2004 and 2005 planting seasons. Application of phosphorus was at 30, 60, 90 and 120 kg ha of P O. As P level increased numbers of petioles, 1 25 pods, nodules, seed/pod, leaf area and yield significantly increased. Disease incidence and severity of Brown Blotch were significantly reduced at 90 and 120 kg ha of P O was not affected irrespective of the method of 1 25 application. Application method did not affect yield. Reduction in brown blotch disease at higher levels of P was recorded.
Evaluation of Different Cowpea Varieties and Genotypes
The present investigation on study of “Evaluation of different cowpea varieties and genotypes” was carried out during summer season in the year 2014-2015 The study was under taken on 30 varieties and genotypes of cowpea using randomized block design with three replication. The variety and genotypes found to be varied growth, yield and quality parameters. Among these 30 types maximum plant height was recorded in variety Akola selection (70.40 cm) were as minimum plant height was recorded in genotype CL-5 (26.33 cm). For days required to first flowers, maximum days taken by genotype CL-23 (71.33 days) were as the minimum days required for variety Vanita (40.80), similarly for days to 50 % flowering, genotype CL-23 recorded (75.40 days) and minimum for variety Vanita taken (45 days ) . For number of green pods per cluster, maximum was recorded in AKCP-20 (3.53) followed by CL-14, were as was minimum was recorded in CL-10 (2.40). Similarly for number of pods per plant, maximum number of pods were recorded in CL-14 (87.80) followed by AKCP-20 (71.73), were as minimum was recorded in AKCP-99 (38.70). In case of Pod length, maximum was recorded in Gadchiroli local-2 (25.60) followed by Akola selection (25 cm) and minimum was recorded selection-5 (11.93). For yield characters average pod weight maximum pod weight for recorded in CL-24 (8.55 gram) were minimum was recorded in Gomati (4.10 gram).For yield per hectare maximum was recorded in CL-14 (157.69 quintal) minimum was recorded in Gomati (71.93 quintal). For protein content maximum protein content was recorded Arka samrudhi (23.80%) was minimum was recorded in Gadchiroli local-2 (15.88 %).
On the fractionation of β-lactoglobulin and α-lactalbumin
Abstract Existing methods for the preparation of α-lactalbumin and β-lactoglobulin are examined critically. It is proposed that the most satisfactory method for the preparation of the whey protein fraction from bovine milk is (NH 4 ) 2 SO 4 fractionation. A number of new gentle procedures for the fractionation of the whey proteins are compared with some existing methods. The distribution of total protein at various stages is determined by total Kjeldahl nitrogen and the kind of protein by starch-gel electrophoresis. The effect of variables, such as pH, salt concentration and temperature on the separation of α-lactalbumin and serum albumin from β-lactoglobulin is studied. Differences in the behavior ( e.g. solubility) of genetic variants of β-lactoglobulin are noted. The solubility of each of the major whey proteins, under similar conditions to those used in the fractionation, is compared with its solubility in the whey protein mixture. The understanding gained of factors important in the fractionation is used in recommending new procedures for α-lactalbumin and β-lactoglobulin isolation.