Enhancement of mycelial growth and polysaccharide production in Ganoderma lucidum (the Chinese medicinal fungus, `Lingzhi') by the addition of ethanol
Methanol, ethanol, 1-propanol and 2-propanol, at 1.5% (v/v), enhanced the growth and polysaccharide production of Ganoderma lucidum. Ethanol was the most effective at 1.5% (v/v) for increasing the biomass production, however, the maximal polysaccharide concentration was produced with 2% (v/v) ethanol in the medium. There was no new polysaccharide component produced by the addition of ethanol.
Ganoderic acid produced from submerged culture of Ganoderma lucidum induces cell cycle arrest and cytotoxicity in human hepatoma cell line BEL7402
Ganoderic acid (GA), produced by submerged culture of Ganoderma lucidum, at 500 μg/ml, caused nearly a 70% inhibition of the growth of human hepatoma cell line BEL7402 but not of a normal human liver cell line L02. Flow cytometry analyses showed that GA blocked the BEL7402 cell cycle at the transition from G1 to S phase.
Women's health in prison: urgent need for improvement in gender equity and social justice.
The needs of women held in detention have received little attention and con-tinue to be neglected by health systems and prison authorities. One reason for this, too readily accepted until now, is that women prisoners are a clear minor-ity group within prisons all over the world. It is only recently that attention has been drawn to the fact that a minor-ity status does not justify the widespread ignorance of women’s basic rights and the considerable gender insensitivity still dominating criminal justice systems.In Europe alone, there are about 100 000 women and girls in prison.