Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C18 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.
Determination of Peoniflorin and Benzoic Acid in Duration of Cultivation of Plant in Radix Paeoniae Rubra
Objective:To compare the contents of the peoniflorin and benzoic of Radix Paeoniae Rubra in the different growth years.Methods:The HPLC was used for determination contents of peoniflorin and benzoic acid of Radix Paeoniae Rubra in different time limits.Results:The sequence of peoniflorin's content of Radix Paeoniae Rubra in different time limit was:biennial triennial annual.The sequence of benzoic acid was annual triennial biennial.Conclusion:There is invariably difference in the Radix Paeoniae Rubra in the different time limit about the peoniflorin and benzoic acid,and provide the evidence of quality control and reasonable collection.It suggests that the best collection should be biennial.
Chemical studies on the oriental plant drugs—XXXIII : The absolute structures of paeoniflorin, albiflorin, oxypaeoniflorin and benzoylpaeoniflorin isolated from chinese paeony root
The absolute structure of paeoniflorin, a major principle of Chinese Paeony root (Paonia albiflora Pallas (Paeoniaceae)) was established as I and as shown in Chart 2 by the X-ray analysis of a bromo derivative (VI) of product K2 acetate (V). The absolute structures of the minor constituents, albiflorin (VII), oxypaeoniflorin (IX) and benzoylpaeoniflorin (X) were also established on the basis of the established structure of paeoniflorin.