Influence of Different Netting Structures on Codling Moth and Apple Fruit Damages in Northwest Croatia
During the vegetation season of 2015 exclusion nets were set up in IPM apple orchard in Krapina (Croatia) to test their effectiveness in preventing the attack of codling moth to apple fruits. Nets were the same in mesh size (2.4 x 4.8 mm) but different in color (white, red, yellow) in order to examine their effect on vegetative growth and quality of apples. To assess the presence of pest, weekly sampling of codling moth on pheromone traps in protected and unprotected (control) net rows was conducted. Single row netting structures resulted in a highly significant reduction of codling moth catches on pheromone traps in comparison to the unprotected control in the experimental orchard. The percentage of codling moth infested fruits during the harvest time was considerably lower, in the protected net rows in comparison with unprotected rows. The lowest percentage of damaged fruits was recorded under the red net (0.96%), followed by white net (1.04%) and yellow net (2.86%). Percentage of damaged fruits in unprotected row amounted to 11.39%. Considering the fact that the mean net efficiency amounted to almost 90%, these results confirm the effectiveness of netting structures in the protection of apple fruits from CM damages.
Molecular phylogeny and population structure of the codling moth (Cydia pomonella) in Central Europe: II. AFLP analysis reflects human-aided local adaptation of a global pest species.
Originally resident in southeastern Europe, the codling moth (Cydia pomonella L.) (Tortricidae) has achieved a nearly global distribution, being one of the most successful pest insect species known today. As shown in our accompanying study, mitochondrial genetic markers suggest a Pleistocenic splitting of Cydia pomonella into two refugial clades which came into secondary contact after de-glaciation. The actual distribution pattern shows, however, that Central European codling moths have experienced a geographic splitting into many strains and locally adapted populations, which is not reflected by their mitochondrial haplotype distribution. We therefore have applied, in addition to mitochondrial markers, an approach with a higher resolution potential at the population level, based on the analysis of amplification fragment length polymorphisms (AFLPs). As shown in the present study, AFLP markers elucidate the genetic structure of codling moth strains and populations from different Central European apple orchard sites. While individual genetic diversity within codling moth strains and populations was small, a high degree of genetic differentiation was observed between the analyzed strains and populations, even at a small geographic scale. One of the main factors contributing to local differentiation may be limited gene flow among adjacent codling moth populations. In addition, microclimatic, ecological, and geographic constraints also may favour the splitting of Cydia pomonella into many local populations. Lastly, codling moths in Central European fruit orchards may experience considerable selective pressure due to pest control activities. As a consequence of all these selective forces, today in Central Europe we see a patchy distribution of many locally adapted codling moth populations, each of them having its own genetic fingerprint. Because of the complete absence of any correlation between insecticide resistance and geographic or genetic distances among populations, AFLP markers do not have a prognostic value for predicting an outbreak of pesticide resistance in the field. By combining mitochondrial genetic data and AFLP analysis it was possible, however, to track the recent evolutionary history of Cydia pomonella on three different time scales: from population splitting in Pleistocene, to interbreeding of mitochondrial haplotypes in Holocene, to human-aided complete intermixing and splitting into many locally adapted populations in very recent times. The case of Cydia pomonella is reminiscent of examples of sympatric speciation and another example of a human-induced globally successful pest species.
The Effectiveness of Two Approaches to the Teaching of High School American History
The purpose of this study was to test the effectiveness of studies in depth utilizing selected materials which had been developed for teaching high school American history.