Study on preparing fatty acid by hydrolyzing Jatropha Curcasl Oil with lipase
Used tween-80 as emulsifier, industrial lipase as catalyst to hydrolyze Jatropha Curcasl Oil and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants as follows: concentration of catalyst 4% (W/W), weight ratio of oil to water 1:4 (W/W), temperature at 40°C, and hydrolysis time is 32h. Under these conditions, the AV(acid value) of hydrolyzed rapeseed oil reached 163.2 mg KOH/g, and hydrolytic rate arrived at 89.6%.
Study on preparing fatty acid by hydrolyzing rapeseed oil with lipase
Used Tween-80 as emulsifier, industrial lipase as catalyst to hydrolyze rapeseed oil and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants as follows: concentration of catalyst 5% (W/W), weight ratio of oil to water 1∶5 (W/W) , temperature at 40°C, and hydrolysis time is 32h. Under these conditions, the acid value of hydrolyzed rapeseed oil reached 175.8 mg KOH/g, and hydrolytic rate arrived at 91.5%.
Synthesis of biodiesel from waste oil by immobilized lipase
Immobilized lipase from Candida sp.99-125 was used for the synthesis of biodiesel from waste oil in organic solvent system. The effect of various factors on the reaction was studied, including the inhibition of short chain alcohols on enzymatic reaction, the nature of solvents, substrate concentration, water content, mode of adding methanol and so on. As can be seen from the results, the conversion rate reached 92% under the optimum conditions, and the life span of the immobilized lipase reached more than 7 batches.