Numerical investigation of the influence of viscoelasticity on drop deformation in shear
Numerical simulations and experimental data are compared for the investigation of the influence of viscoelasticity on drop deformation in shear. A viscoelastic drop suspended in a Newtonian liquid, or a Newtonian drop suspended in a viscoelastic liquid, is sheared and investigated for transients, relaxation after cessation of shear flow, and step‐up in shear rate. The numerical simulations are conducted at parameters chosen to model the experiments. We use the volume of fluid (VOF) continuum surface force (CSF) algorithm for situations dominated by shear. For drop relaxation experiments, we use the paraboloid representation of the interface in the surface tension force (PROST) algorithm. The Oldroyd‐B and Giesekus constitutive models are implemented. An interesting result is that by stepping up in the capillary number gradually, a stationary states is achieved at higher capillary numbers than without the graduated steps. The experimental work is described in Verhulst, Moldenaers and Cardinaels [l]. We pre...
Influence of viscoelasticity on drop deformation and orientation in shear flow. Part 2: Dynamics
Abstract An experimental study of drop dynamics under shear is conducted for five fluid pairs: a reference Newtonian system, two systems with a viscoelastic drop in a Newtonian matrix, one with a Newtonian drop in a viscoelastic matrix, all at drop to matrix viscosity ratio λ = 1.5 , and a separate case at λ = 0.75 . The viscoelastic liquids are either a Boger fluid or a shear-thinning viscoelastic fluid satisfying an Ellis model. Deborah numbers in the range 1–2 and a range of capillary numbers from low to above breakup conditions are addressed. The results focus on three aspects: relaxation after cessation of shear, a new viscoelastic drop breakup scenario, and the effect of shear flow history on drop breakup. Numerical simulations with the 3D volume-of-fluid PROST method complement the experimental results.
Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785
A maltotriose-forming amylase (G3Amy) from Kitasatospora sp. MK-1785 was successfully isolated from a soil sample by inhibiting typical extracellular α-amylases using a proteinaceous α-amylase inhibitor. G3Amy was purified from the MK-1785 culture supernatant and characterized. G3Amy produced maltotriose as the principal product from starch and was categorized as an exo-α-amylase. G3Amy could also transfer maltotriose to phenolic and alcoholic compounds. Therefore, G3Amy can be useful for not only maltotriose manufacture but also maltooligosaccharide-glycoside synthesis. Further, the G3Amy gene was cloned and expressed in Escherichia coli cells. Analysis of its deduced amino acid sequence revealed that G3Amy consisted of an N-terminal GH13 catalytic domain and two C-terminal repeat starch-binding domains belonging to CBM20. It is suggested that natural G3Amy was subjected to proteolysis at N-terminal region of the anterior CBM20 in the C-terminal region. As with natural G3Amy, recombinant G3Amy could produce and transfer maltotriose from starch.