Effects of bis(2-ethylhexyl) phthalate, γ-hexachlorocyclohexane, and 17β-estradiol on the fry stage of medaka (Oryzias latipes).
The effects of bis(2-ethylhexyl) phthalate (DEHP), γ-hexachlorocyclohexane (γ-HCH), and 17β-estradiol (E2) on the fry stage of medaka were investigated. The medaka fry were exposed to different concentrations (0.01, 0.1, 1, and 10μg/L) of these chemicals for 3 weeks after hatching. Then, mortality, body weight, sex ratio, and gonadosomatic index (GSI) of the matured fish (after 5 months) were measured. Mortality was increased significantly in the 10μg/L E2 group. Distortion of sex ratio was found in 1 and 10μg/L E2 groups. DEHP treated groups showed the GSI reduction only in male fish. All the γ-HCH and parts of the E2 treated groups showed the GSI reduction in both sexes. Exposure of DEHP, γ-HCH, and E2 during the fry stage affected normal maturation of medaka at the concentrations which had no impact on mortality or sex ratio.
Developmental profiles and expression of the DNA methyltransferase genes in the fathead minnow (Pimephales promelas) following exposure to di-2-ethylhexyl phthalate
DNA methylation is an epigenetic regulator of gene expression, and this process has been shown to be disrupted by environmental contaminants. Di-2-(ethylhexyl) phthalate (DEHP) and related phthalate esters have been shown to affect development in early life stages of fish and can alter genomic methylation patterns in vertebrates. The objectives of this study were the following: (1) Describe the expression patterns of the DNA methyltransferase (dnmt) genes during early fathead minnow (FHM) development. These genes are critical for methylation and imprinting during development. (2) Determine the effects of DEHP on the development of FHM larvae [1 and 14 days post-hatch (dph)]. (3) Determine the effect of DEHP on dnmt expression and global methylation status in larval FHM. FHMs were first collected over a developmental time course [1, 3, 5, 6, and 14 days post-fertilization (dpf)] to investigate the expression patterns of five dnmt isoforms. The expression of dnmt1 and dnmt7 was relatively high in embryos at 1 dpf but was variable in expression, and these transcripts were later expressed at a lower level (>3 dpf); dnmt3 was significantly higher in embryos at 1 dpf compared to those at 3 dpf. Dnmt6 showed more of a constitutive pattern of expression during the first 2 weeks of development, and the mRNA levels of dnmt8 were higher in embryos at 5 and 6 dpf compared to those at 1 and 3 dpf, corresponding to the hatching period of the embryos. A waterborne exposure to three concentrations of DEHP (1, 10 and 100 µg/L) was conducted on 1-day FHM embryos for 24 h and on larval fish for 2 weeks, ending at 14 dpf. DEHP did not negatively affect survival, hatch rate, or the expression of dnmt isoforms in FHMs. There were no differences in global cytosine methylation following DEHP treatments in 14 dpf larvae, suggesting that environmentally relevant levels of DEHP may not affect global methylation at this stage of FHM development. However, additional targeted methylome studies are required to determine whether specific gene promoters are differently methylated following exposure to DEHP. This study offers new insight into the roles of the dnmt enzymes during FHM development.
Production of xylanase by Aspergillus awamori on complex medium in stirred tank and airlift tower loop reactors
Abstract Aspergillus awamori was cultivated on complex medium containing wheat bran as sole carbon source in the precultures as well as in the main cultures in stirred tank and airlift tower loop reactors. The decomposition of the wheat bran particles by α -amylase and xylanase produced by the fungus was investigated by monitoring the enzyme activities, the CO 2 production rate, the concentrations of reducing sugars, glucose, xylose, phosphate and ammonium as well as by applying transmission electron microscopy. By applying synthetic medium in the preculture, the time lag was longer, the growth faster, the xylanase activity higher in the main culture, than by using wheat bran in the preculture. With increasing the stirrer speed the intimate contact between the pellets and the wheat bran was reduced, which caused a decrease of the growth and the xylanase production. By applying ground wheat bran, filamentous mycelium was formed, the growth and the substrate uptake rates increased, but the xylanase production decreased due to the less intimate contact between the fungus and the wheat bran. In the airlift tower loop reactor filamentous mycelium was formed due to the low specific power input. In spite of the high medium viscosity, high xylanase productivity was obtained. With ground wheat bran the particles were completely covered by filamentous mycelium, medium viscosity strongly increased and as consequence the growth and xylanase production decreased.