Micropropagation of Capparis decidua through In Vitro Shoot Proliferation on Nodal Explants of Mature Tree and Seedling Explants
In vitro clonal propagation of Capparis decidua was achieved using nodal explants from mature trees, and cotyledonary node, cotyledon and hypocotyl explants taken from the seedlings. Explants cultured on MS medium supplemented with BAP showed differentiation of multiple shoots and shoot buds in 4–5 weeks in the primary cultures. The medium with BAP (5 mg/l) was the best for shoot bud proliferation from the nodal as well as seedling explant. Shoot multiplication was best on cotyledonary node. In the nodal explants shoot multiplication was best on medium supplemented with 5 mg/l BAP and after second subculturing further multiplication of shoot buds was highest on the medium containing 3 mg/l BAP. Shoots were separated from mother cultures in each subculturing for rooting. Rooting was best achieved using 1 mg/l IBA in the medium. Rooted plantlets were transferred td earthen pots with garden soil and peat moss mixture.
In vitro propagation of caper (Capparis spinosa L.)
SummaryA twenty fold multiplication per twenty days of caper was achieved by culturing nodal shoot segments in the presence of BAP (4 μM) plus IAA (0.3 μM) and GA3 (0.3 μM). The use of a modified MS medium facilitated this response. Plantlet regeneration was induced on single shoots taken from proliferating clusters subcultured for 20 days on a reduced BAP (2 μM) without auxin and gibberellin Higher rooting responses (70%) were obtained after a 20-day incubation period in darkness on solid half-strength MS1 medium plus IAA (30 μM), followed by a subsequent 20 day culture period on half-strength MSI basal medium. Proliferation was mainly due to axillary shoot-bud development as revealed by histological studies. The extensive meristematic activities observed indicated the enormous morphological potential of this species.
Induction of Callus Tissues Initiation from Different Somatic Organs of Rice Plant by Various Concentration of 2.4-dichlorophenoxy acetic acid
Callus tissues were induced from different somatic organs of O. sativa variety Taichung No. 65. Specific 2.4-dichlorophenoxy acetic acid (2.4-D) concentration in a medium is necessary for the induction of callus from different organs. The optium 2.4-D concentration for callus induction from different organs are 0.5ppm for roots, 2ppm for scutellum and cotyledonous node, 6ppm for coleoptile and 8ppm for stem nodes and leaf-sheath. All cultures were incubated in darkness at 28°C.Anatomical studies showed that the initiation of callus tissues from leaf-and coleoptile are initiated from the meristematic cells located very closely by the immature vascular bundles. The initiation of callus in nodes seems to be similar to those of adventitious roots which are initiated near to the differentiating vascular tissue or directly from the procambium.