Simultaneous Qualitative and Quantitative Study of Main Compounds in Commelina communis Linn. by UHPLC-Q-TOF-MS-MS and HPLC-ESI-MS-MS.
This study reported the identification and determination of the main components of Commelina communis Linn. A total of 62 compounds were identified in C. communis Linn. extract, which included 29 flavonoids and flavonoid glycosides, 17 phenolic acids, 4 alkaloids, 1 pyrimidine alkaloids, 3 sterols and 8 fatty acids and others by ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Moreover, a specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C. communis Linn., which included orientin, iso-orientin, vitexin, isovitexin, rutin, apigenin, protocatechuate, vanillic acid, caffeic acid, ferulic acid, luteolin, quercetin and isorhamnetin. All calibration curves showed good linearity (r ≥ 0.9991) within the test range. The intra- and inter-day precisions (relative standard deviation%, RSD%) were within 1.04 and 0.92%, and the recoveries ranged from 98.64 to 100.8%. These results may contribute to the further study and quality control for C. communis Linn.
Determination of Iso-Orientin in Commelina communis from Different Growing Periods by HPLC
Objective: To establish a accurate method for determination of iso-orientin in Commelina communis L.and compare the determination on different growing periods in order to determine the optimal harvesting time.Method: The Agilent TC-C18(4.6 mm × 250 mm,5 μm) was used.The mobile phase was acetonitrile-0.1% phosphoric acid solution(15∶ 85) and the flow rate was 1 mL·min-1 with UV detected.Result: The linear ranges of were at 0.08～1.00 μg,the average recoveries were 101.2%.Different growth stages have a significant effect for determination of iso-orientin and the levels of maturity is a highest.Conclusion: The method is convenient,effective and suitable for determination of iso-orientin in C.communis.It provides the basis for the quality standards and reference in order to determine the optimal harvesting time.
Bone changes in tuberous sclerosis mimicking metastases.
Sclerotic and lytic bone changes of tuberous sclerosis (TS) can mimic bone metastases. We report a case of bone metastases from bronchogenic carcinoma in a patient with TS bone changes. Bone scintigraphy and magnetic resonance imaging are useful in distinguishing the TS bone lesions from bone metastases.