Nomenclature for factors of the HLA system, update November 2006 *
The following sequences have been submitted to the Nomenclature Committee since the October 2006 nomenclature update and, following agreed policy, have been assigned official allele designations. Full details of all sequences will be published in a forthcoming report. Below are listed the newly assigned sequences (Table 1) and confirmations of previously reported sequences (Table 2). The accession number of each sequence is given and these can be used to retrieve the sequence files from either the EMBL, GenBank or DDBJ data libraries.
Identification and frequency of a novel HLA-A allele, A*110104.
In this study, we identified a novel HLA-A allele A*110104, which was found in a Chinese family. The full-length complementary DNA cloning of HLA-A locus and subsequently sequence-based typing showed that A*110104 was identical to A*110101 except for a nucleotide substitution of CAC to CAT at codon 93 without an amino acid replacement. The frequency of A*110104 in Chinese population (n = 1200) were approximately 0.083%.
Identification, sequencing and serology of HLA-B*3527.
We describe a variant HLA-B*35 allele (B*3527) that was detected by the polymerase chain reaction using sequence-specific primers (PCR-SSP) in an individual on the Welsh Bone Marrow Donor registry. B*3527 differs from B*3501 by a single base (G/A) at position 302 that encodes an amino acid change of Ser to Asn at position 77 in the Bw6 epitope. Serological studies using 38 B35-, 4 Bw6- and 7 Bw4-reactive sera indicated that the B*3527 product was indistinguishable from the B35 specificity, and that the substitution did not significantly affect the Bw6 epitope. A family study determined the B*3527 bearing haplotype as: HLA-A*29, B*3527, Cw*0401, DRB1*0404, DRB4*0101/0103, DQA1*03, DQB1*0302, BFS, C4A3, C4B1. The phenotype and gene frequencies in the local Welsh population were<0.01% and <0.00005%, respectively.