Quantitative estimation of gossypurpurin in the isolation of gossypol
It has been found that the relative amounts of free gossypol contained in the products of the extraction of defatted high-gossypol cottonseed flour by chloroform, diethyl ether, and acetone falls with an increase in the polarity of the solvents. It has been shown that, in addition to gossypol, gossypurpurin is extracted. Its highest amount (4.82%) was found in an acetone extract, and its lowest amount (2.68%) in an ethereal extract.
Detection of a Toxic Phenolic Compound in Cottonseed Extract and its Products
2 Abstract: Cottonseed is a good source of high quality meal and edible oil. However, it contains a toxi c phenolic compound called gossypol. Gossypol is an anti-nutritious factor that limits the use of cottonseed and its products due to its toxicity associated to its reactions with amino acids and minerals. This is the first study on gossypol ever conducted in Pakistan. The purpose of this study was to estimate the level of gossypol in oil and ghee (Hydrogenated oil) samples from local markets so that awareness can be created regarding maintenance of minimum gossypol level in edible oils. During this study gossypol was extracted from cottonseeds and cotton seed cake using different organic solvents. The compound was detected in extracts by applying Chromatographic technique as well as chemical tests with SbCl3, Pb(CH3COO)2 and SnCl3. Spectrophotometric techniques were also employed for quantitative analysis by measurin g absorbance of samples at wavelength of 290 nm. It was found that overall the contents of gossypol were higher in cottonseed oils as compared to Ghee (hydrogenated oil).
The occurrence of (+) gossypol inGossypium species
Abstract(+) Gossypol, 2,2′-bi[8-formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthyl], has been isolated as the (+) dianilino derivative from cottonseed grown in widely different areas of the cottonbelt of the United States. The optically active derivative obtained had rotations varying from 43% to 54.7% of the value reported for the optically pure enantiomer. Attempts to resolve racemic gossypol by chemical means were not successful, but (+) gossypol was obtained by solvent fractionation (7.2%) and by chromatography on β-lactose (2.2%).