Quantitative estimation of gossypurpurin in the isolation of gossypol
It has been found that the relative amounts of free gossypol contained in the products of the extraction of defatted high-gossypol cottonseed flour by chloroform, diethyl ether, and acetone falls with an increase in the polarity of the solvents. It has been shown that, in addition to gossypol, gossypurpurin is extracted. Its highest amount (4.82%) was found in an acetone extract, and its lowest amount (2.68%) in an ethereal extract.
Cytotoxicity analysis by MTT assay of isolated Gossypol from Bt and Non-Bt Cotton Seeds on HeLa Cell Lines
The present paper deals with study of In-vitro cytotoxicity effect of isolated gossypol from Bt and Non-Bt cotton seeds on HeLa cell lines. Gossypol is a polyphenolic binaphthyl diadehyde natural yellow colored pigment. It is not only resistance substance for cotton plant’s self –defense system against insect pests and possibly some diseases but also an important phytochemical compound of immense interest due to its several biological properties including anti-cancer,anti-HIV,anti-oxidation, antimicrobial and as male contraceptive. During this study gossypol exhibited broad spectrum of anti-cancer activity against the HeLa cell lines. The cytotoxicity effect of gossypol was detemined by MTT (3-(4-,5-dimethylthiazolyl-2)-2,5-dipheniltetrazolium bromide) assay. Gossypol from Bt and Non-Bt cotton seeds has shown dose dependent cytotoxicity effect against HeLa cell lines. In-vitro screening of the gossypol showed potential cytotoxic activity against HeLa cell lines. Mortality rate of 11.5884% and 22.6058% with 3μg/1μl concentration of isolated gossypol from Bt and Non-Bt cotton seed extracts was observed on HeLa cell lines respectively. Hence the inhibitory concentration at 50% (IC50) was fixed at 3μg/1μl of gossypol for HeLa cells. The standard anti-cancer drug Doxorubicin (1mg/ml) was also used in this study to confirm anti-cancer activity of gossypol isolated from Bt and Non-Bt cotton seed with 1.7828% cell viability. The present study confirms the mild toxic effect of gossypol on HeLa cell lines and can preferably be used as anti-cancerous drug in combination with other natural similar compounds to replace the synthetic chemical drugs for fewer side effects.
Techno-economic analysis of chemical looping combustion with humid air turbine power cycle
Abstract Power generation from fossil fuel-fired power plant is the largest single source of CO2 emission. CO2 emission contributes to climate change. On the other hand, renewable energy is hindered by complex constraints in dealing with large scale application and high price. Power generation from fossil fuels with CO2 capture is therefore necessary to meet the increasing energy demand, and reduce the emission of CO2. This paper presents a process simulation and economic analysis of the chemical looping combustion (CLC) integrated with humid air turbine (HAT) cycle for natural gas-fired power plant with CO2 capture. The study shows that the CLC–HAT including CO2 capture has a thermal efficiency of 57% at oxidizing temperature of 1200 °C and reducer inlet temperature of 530 °C. The economic evaluation shows that the 50 MWth plant with a projected lifetime of 30 years will have a payback period of 7 years and 6 years for conventional HAT and CLC–HAT cycles respectively. The analysis indicates that CLC–HAT process has a high potential to be commercialised.