Data of PCL-b-P(MMA-DMAEMA)2 characterization and related assays
The data presented here are related to the research paper entitled “PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors” by Franco et al. [1]. Characterization data of PCL-diol, macroinitiator Br-PCL-Br, homopolymers (PMMA and PDMAEMA) and copolymers (batch 1 and batch 2) analyzed by FTIR, SEC and NMR, as well as, characterization of PCL-NS formulation by laser diffraction and DLS analysis, initial nanocapsule formulations and 1C-NC and 2C-NC formulations, including hydrodynamic diameter at different pH media, and DMSO cytotoxicity.
A novel method for DNA delivery into bacteria using cationic copolymers
Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described. The reversible addition-fragmentation chain transfer (RAFT) polymer synthesis technique and the reversible deactivation radical polymerization variants allow the design of polymers with well-controlled molar mass, morphology, and hydrophilicity/hydrophobicity ratios. RAFT was used to synthesize two amphiphilic copolymers containing different ratios of the amphiphilic poly[2-(dimethyl-amino) ethyl methacrylate] and the hydrophobic poly [methyl methacrylate]. These copolymers bound to pUC-19 DNA and successfully transfected non-competent Escherichia coli DH5α, with transformation efficiency in the range of 103 colony-forming units per µg of plasmid DNA. These results demonstrate prokaryote transformation using polymers with controlled amphiphilic/hydrophobic ratios.
Urban Identities and Catastrophe: Izmir and Salonica at the End of the Ottoman Empire*
Two huge fires dramatically influenced the urban development of Salonica and Izmir, in 1917 and in 1922, respectively. These catastrophes occurred after the Ottoman Empire fell, and Salonica and Izmir had shifted into new national contexts. The fires mainly destroyed the districts that were transformed during the late‐Ottoman period. These districts became the cosmopolitan façades of modern Izmir and Salonica. The post‐fires' tabula rasa provided an opportunity for Greek and Turkish politicians and European planners to change the urban identity of both cities. Moreover, the Lausanne Treaty of 1923 transformed their multicultural societies also. Reconstruction plans had been thought to de‐Ottomanize and remove the previous idea of the towns and their multiplicity, interpreting new cultural and nationalist feeling. Here, I emphasize how modernity was interpreted before and after the fires, and point out contradictions between ideological aspects of planning and how the first urban districts were built during the 1920s.