Influence of Different Netting Structures on Codling Moth and Apple Fruit Damages in Northwest Croatia
During the vegetation season of 2015 exclusion nets were set up in IPM apple orchard in Krapina (Croatia) to test their effectiveness in preventing the attack of codling moth to apple fruits. Nets were the same in mesh size (2.4 x 4.8 mm) but different in color (white, red, yellow) in order to examine their effect on vegetative growth and quality of apples. To assess the presence of pest, weekly sampling of codling moth on pheromone traps in protected and unprotected (control) net rows was conducted. Single row netting structures resulted in a highly significant reduction of codling moth catches on pheromone traps in comparison to the unprotected control in the experimental orchard. The percentage of codling moth infested fruits during the harvest time was considerably lower, in the protected net rows in comparison with unprotected rows. The lowest percentage of damaged fruits was recorded under the red net (0.96%), followed by white net (1.04%) and yellow net (2.86%). Percentage of damaged fruits in unprotected row amounted to 11.39%. Considering the fact that the mean net efficiency amounted to almost 90%, these results confirm the effectiveness of netting structures in the protection of apple fruits from CM damages.
Genetic architecture in codling moth populations: comparison between microsatellite and insecticide resistance markers
The codling moth, Cydia pomonella, is renowned for developing resistance to insecticides and causing significant economic damage to pome fruits worldwide. In spite of its economic importance, little is known about the patterns of movement of this pest and the effects of insecticide treatment on the population genetic structure. Here, we investigated the genetic structure of the pest in 27 orchards from France, Italy, Armenia and Chile at seven microsatellite loci and two resistance markers [biochemical activity of cytochrome P450 oxidases and proportion of knockdown resistance (kdr) alleles in the sodium channel gene]. According to the microsatellite loci, we detected isolation by distance at the supranational scale but found no evidence of geographical structure among the 24 French orchards, which were mainly structured by the intensity of the insecticide treatments. Similarly, the highest levels of metabolic resistance associated with activity of the cytochrome P450 oxidases were detected in the most treated orchards. The kdr alleles were observed in southern France and Armenia where the pyrethroid insecticides were or have been intensively sprayed. The intensity of the insecticide treatments marginally affected the allelic richness in each orchard, but not the level of inbreeding. These results suggest important and high‐distance gene flow among the codling moth populations, which were mainly structured according to the history of insecticide applications. Differences in mutation‐migration‐drift equilibrium among treated and untreated orchards also suggest that insecticide applications are the main force regulating the local dynamics of codling moth populations.
The infection process, sporulation and survival of Alternaria helianthi on sunflower
SUMMARY 
 
Electron microscopy was used to study the infection of sunflower leaves by Alternaria helianthi. Conidia germinated by producing one to many germ tubes which grew across the leaf surface before forming appressoria. The fungus directly penetrated its host through the cuticle and epidermis. Entry into the host through wounds and stomates was also observed. Extracellular sheaths were found to be associated with germ tubes and intercellular hyphae of A. helianthi. Conidiophores developed through collapsed stomates, from leaf veins, trichomes and also from mycelium growing across the host leaf surface. Microcylic conidia were produced directly from parent conidia under certain conditions. Studies using a volumetric spore trap showed that the airborne spore concentration followed a distinct periodicity with peaks occurring between 0900 and 1100 h each day. Laboratory studies showed that safflower, noogoora burr and bathurst burr could serve as alternative hosts for A. helianthi. The pathogen was readily isolated from sunflower crop debris from a diseased crop that had been harvested 1 yr earlier.