Comparing interfertility data with random amplified microsatellites DNA (RAMS) studies in Ganoderma Karst. Taxonomy
The taxonomy of the causal pathogen of basal stem rot of oil palms, Ganoderma is somewhat problematic at present. In order to determine the genetic distance relationship between G. boninense isolates and non-boninense isolates, a random amplified microsatellites DNA (RAMS) technique was carried out. The result was then compared with interfertility data of G. boninense that had been determined in previous mating studies to confirm the species of G. boninense. Dendrogram from cluster analysis based on UPGMA of RAMS data showed that two major clusters, I and II which separated at a genetic distance of 0.7935 were generated. Cluster I consisted of all the biological species G. boninense isolates namely CNLB, GSDK 3, PER 71, WD 814, GBL 3, GBL 6, OC, GH 02, 170 SL and 348781 while all non-boninense isolates namely G. ASAM, WRR, TFRI 129, G. RES, GJ, and CNLM were grouped together in cluster II. Although the RAMS markers showed polymorphisms in all the isolates tested, the results obtained were in agreement with the interfertility data. Therefore, the RAMS data could support the interfertility data for the identification of Ganoderma isolates.
Ganoderma Diseases of Perennial Crops
This history of Ganoderma taxonomy phylogeny development of detection methods Ganoderma in southern South America - an approach to its systematics status of Ganoderma on oil palm in Indonesia Ganoderma diseases of tea - current status in Malaysia status of Ganderma diseases of forest trees in India the spread of Ganoderma from infective sources in the field some insects associated with Ganoderma fruiting bodies the use of soil amendments for control potential biocontrol agents for control in the field in Indonesia breeding for resistance to Ganoderma wilt of coconut in India.
Genetic Variation in Fusarium moniliforme Isolated from Seeds of Different Host Species from Burkina Faso Based on Random Amplified Polymorphic DNA Analysis
Random amplified polymorphic DNA (RAPD) analysis was used to assess genetic variation among 28 isolates of Fusarium moniliforme isolated from seed samples of six host species (rice, sorghum, maize, cotton, hibiscus and parkia) collected in Burkina Faso. Score-able markers (128) were obtained with 20 random decamer primers. Cluster analysis with UPGMA using genetic distances showed that the isolates were of five main groups. In general, isolates from the same host species were found to group together in the same cluster. The isolates from cotton and hibiscus were more closely related to each other than to the isolates from sorghum, rice and maize. Results indicate that host preference is possibly a factor in the differentiation of isolates.