Epidemiologic Aspects of a Foot- and-Mouth Disease Epidemic in Cattle in Ecuador
A case-control study was conducted to identify herd-level risk factors associated with foot-and-mouth disease (FMD) in Ecuador. Case herds were those with presence of cattle with clinical signs of FMD and that tested positive for FMD virus during the epidemic of 2002 (n = 39). Control farms (n = 78) were selected randomly from a list of farms without a history of FMD. All study farms were visited, and managers were interviewed to complete an epidemiologic questionnaire. Feedlot operations, purchase of livestock at markets, and close proximity to markets or slaughter facilities were identified as risk factors for FMD (P ≤ 0.01). In addition to systematic vaccination of the national herd, we recommend allocation of funds for ring-vaccination of cattle herds in close proximity to markets or slaughter facilities, and to enforce movement of vaccinated-cattle only to help control and prevent new epidemics of FMD.
Foot-and-Mouth Disease Virus Typing by Complement Fixation and Enzyme-Linked Immunosorbent Assay using Monovalent and Polyvalent Antisera
An indirect “sandwich” enzyme-linked immunosorbent assay (ELISA) using polyvalent and monovalent antisera was compared with the 50% complement fixation (CF) test for the detection of foot-and-mouth disease (FMD) O, A, and C virus types. ELISA was more sensitive than CF tests when polyvalent antisera were used for detecting the 3 types of virus in epithelial samples, whereas ELISA using monovalent antisera was the least sensitive technique. The ELISA performed with polyvalent antisera was 9 times more sensitive for detecting FMD virus than that with monovalent antisera. However, viral isolation in cell culture was the most sensitive detection system. The combined use of ELISA with polyvalent antisera and cell culture inoculations was the most effective procedure for identifying FMD virus in epithelial samples from the field.
Rapid determination of the effectiveness of insect resistant packaging
A technique was developed to evaluate the usefulness of odour barriers and to determine the effect of package content in consumer size packages on resistance to infestation by insects. Female Plodia interpunctella (Hubner) could distinguish sealed packets containing food from non-food items as a suitable site for oviposition. Utilization ofthis technique will reduce the time needed to evaluate new materials for use in insect resistant packaging.