Effect of plasticizer type and amount on hydroxypropyl methylcellulose-beeswax edible film properties and postharvest quality of coated plums (cv. Angeleno).
The effect of the composition of hydroxypropyl methylcellulose (HPMC)-beeswax (BW) edible coatings on stand-alone film properties and on postharvest quality of coated 'Angeleno' plums was studied. Glycerol (G) and mannitol (M) were tested as plasticizers at two different plasticizer/HPMC ratios (100:1 and 300:1 molar basis). BW content was 20 or 40% (dry basis). An increase in G content increased film flexibility and vapor permeability (WVP), whereas an increase in M content enhanced film brittleness without affecting WVP. An increase in BW content reduced film flexibility and reduced WVP of only G-plasticized films. Coatings reduced plum softening and bleeding, but were not effective in reducing plum weight loss. At low plasticizer content, coatings reduced texture loss effectively. Low BW also lowered plum bleeding. Plasticizer type affected only ethanol and acetaldehyde contents without affecting the remaining quality parameters. Therefore, HPMC-BW coatings have the potential to extend the shelf life of plums. However, this effect depends on coating composition. Differences between coating and film performance indicate that data from stand-alone films may be used as a preliminary screening, but coating performance should be analyzed on coated fruit.
Susceptibility to Chilling Injury of Peach, Nectarine, and Plum Cultivars Grown in California
The susceptibility to chilling injury (CI) or internal breakdown (IB) was evaluated in the most currently planted yellow- and white-flesh peach (Prunuspersica (L.) Batsch) and nectarine (Prunus persica var. nectarine (L.) Batsch) and plum (Prunus salicina Lindel) cultivars from different breeding sources and fruit types. Cultivars were segregated into three categories (Cat. A, B, and C) according to their susceptibility to CI or IB symptoms (mealiness and flesh browning) when exposed to 0 OC or 5 OC storage temperatures. Cultivars in Cat. A did not develop any symptoms of CI after 5 weeks of storage at either temperature. Cultivars in Cat. B developed symptoms only when stored at 5 "C within 5 weeks of storage. Cultivars were classified in Cat. C when fruit developed CI symptoms at both storage temperatures within 5 weeks of storage. Most of the yellow- and white-flesh peach cultivars developed IB symptoms when stored at both storage temperatures (Cat. C). Most of the new nectarine cultivar introductions did not develop CI symptoms when stored at 0 OC or 5 OC after 5 weeks (Cat. A). Three out of six plum cultivars tested had CI symptoms within 5 weeks storage at 0 OC. However, all of the plum cultivars tested developed CI symptoms when stored at 5 OC (Cat. B). The importance of proper temperature management during postharvest handling was demonstrated.
Diagnostic and therapeutic studies on chronic diarrhea in dairy animals
The study was conducted to determine the etiology of chronic diarrhea in dairy animals. Seven causes of chronic diarrhea were established in dairy cattle and buffaloes. Balantidiasis in 37.6 per cent, dietary diarrhea in 9.9 per cent, chronic salmonellosis in 6.9 per cent cases of chronic diarrhea, secondary copper deficiency and amphistomiasis in 6.9 per cent, clinical Johne‘s disease in 3.9 per cent and chronic peritonitis in 2.97 per cent of dairy animals, manifest chronic diarrhea. The precise diagnosis could not be established in 24.50 per cent of animals. Lymphosarcoma was confirmed in one of the dairy cattle. Body condition score was associated to the etiology of chronic diarrhea. B. coli infected cattle and buffalo revealed cysts in 32.3 per cent cases while 5 per cent positive cases revealed both cysts and trophozoites. Mean cyst count was 2.73±0.91 hpf in diarrheic animals compared to 0.11±0.447 hpf in healthy animals. Clinical Johne‘s disease was confirmed in four animals. Hematological changes revealed anemia and neutrophilia with morphological changes suggestive of regenerative to degenerative left shift. High plasma phosphorus, hypoproteinaemia and hypoalbuminaemia was seen in clinical Johne‘s disease. Salmonella isolates showed higher in-vitro sensitivity to enrofloxacin (87%), ciprofloxacin (87%), gentamicin (75%), and tetracycline (66.7 %) and were resistant to ampicillin and amoxicillin. Mean cyst count reduction from 2.41±1.16 to 0.11±.33 was observed in oral metronidazole treated group where as reduction of mean cyst count from 2.73±0.918 to 0.18±0.44 in intravenous metronidazole treated group. Recovery rate with oral and intravenous metronidazole was 42.1 and 63.1 per cent respectively. Recovery results in significant decrease in eiosionphil count and increase in total plasma protein in both oral and intravenous treatment groups. Complete recovery response approaches 60 per cent in balantidiasis affected adult dairy animals. Balantidiasis was the major cause of chronic diarrhea and intravenous administration of metronidazole was more effective than oral metronidazole.