Alkaloids from Lupinus argenteus var. stenophyllus.
TLC and GLC of an alkaloid extract of the aboveground portions of Lupinus argenteus Pursh. var. stenophyllus (Rydb.) Davis (Leguminosae) suggested the presence of sparteine, beta-isosparteine, delta5-dehydrolupanine, alpha-isolupanine, lupanine, thermopsine, and anagyrine. GLC-mass spectrometry confirmed these preliminary findings. Preparative TLC was used to isolate sparteine, and this alkaloid was further characterized by IR spectral analysis and derivatization.
Alkaloid diversity in eleven species of Ormosia and in Clathrotropis macrocarpa (Leguminosae-Papilionoideae)
Quinolizidine alkaloids were surveyed using gas chromatography (GC) and combined mass spectroscopy (MS) in bark or leaves of eleven species ofOrmosia Jackson as well as inClathrotropis macrocarpa Ducke from tropical forests in Latin America. A list of detected alkaloids, with their MS fragmentation patterns and GC retention indices, is given, as are the distribution of alkaloids among the samples and their detected concentrations. Alkaloid diversity inOrmosia was high, and the taxonomic distribution of specific alkaloids was not congruent with morphological taxonomic criteria: Of 143 alkaloids apparent in 14 samples, 65 were observed in only one sample, and no single alkaloid was common to all samples. Within a species, alkaloid concentrations were inconsistent and therefore cannot be used as a taxonomic character. This was shown for leaf alkaloid concentrations of two neighboring conspecific trees in the same habitat.ResumenHemos analizado mediante cromatografia de gases (CG) y espectrometría de masas (EM) la composición en alcaloides quinolizidínicos de muestras de corteza y hojas de once especies deOrmosia Jackson y deClathrotropis macrocarpa Ducke, procedentes de las selvas de Latinoamérica. Presentamos una lista completa de los alcaloides detectados incluyendo sus fragmentos en EM y su índice de retención en CG. También presentamos la distribución de los alcaloides en las muestras y su concentración. La variedad de alcaloides era alta y su distribución por especies no se ajustó con criterios taxonómicos morfológicos: De los 143 alcaloides detectados en 14 muestras, 65 alcaloides estaban presentes en una sola muestra y ningún en todas las muestras. La concentración de los alcaloides en una especie no fue consistente y por lo tanto no puede utilizarse como carácter taxonómico. Esta conclusión proviene del análisis de la concentración de alcaloides en hojas de dos árboles de la misma especie en el mismo hábitat.
Two new alkaloids in cacti.
The plant Thelocactus aacromeris (Engelm.) L. Benson, Coryphantha. macromeris (Engelm.) Lem., Britton and Rose, has been found to contain three alkaloids by gas chromatography. The alkaloids were extracted on a large sc8le from the plant by standard methods.a The principal alkaloid was separated from the minor constituents by chromatography on alumina, The main alkaloid, "macramerine", gave mol. wt. 240 2 25 GLCS, (213 vapor pressure lowering), m.p. 66-67.5" and rotation of ( [o]Es 147.01, c = 0.0390 g/ml, CHCl,; PIBS 42.61, c 0.0200 g/ml, absolute ethanol). The structure, according to spectral data discussed below, is (I):