Evaluation of the safety of domestic food preparation in Malaysia.
Food-handling practices were studied in 119 and 158 households, respectively, in an urban and a rural community in Peninsular Malaysia. Hazard analyses, including microbiological analysis of foods, were carried out in two households in each community and in a house that prepared food for distribution in the urban area. Kitchen hygiene was generally acceptable, although rated "poor" in some instances in the rural area. Food prepared for lunch was usually sufficient for dinner also, the leftover items being stored at ambient temperature until required. In the house that prepared food for distribution, breakfast was prepared during the evening, stored at ambient temperature overnight, and reheated before sale the next morning. There was a local preference for cooking food at temperatures close to boiling point; this reduced the numbers of vegetative cells but not those of spores. In some stored foods the populations of Staphylococcus aureus, Bacillus cereus and mesophilic aerobic bacteria increased, the last-mentioned reaching spoilage levels. Reheating reduced the populations of proliferating bacteria in most foods to acceptable levels but would not have destroyed heat-resistant enterotoxins. Because of their importance in combating acute bacterial foodborne disease, the control of the temperature and time factors during the cooking and storage of food should receive special attention in education on health and food safety.
Food safety knowledge and attitudes: culture and environment impact on hawkers in Malaysia. Knowledge and attitudes are key attributes of concern in hawker foodhandling practices and outbreaks of food poisoning and their prevention.
Abstract This study determined the impact of culture and environment on knowledge and attitudes of hawkers towards food safety, foodborne illnesses and their prevention. One hundred hawkers in Kuala Lumpur (KL) were interviewed, using a structured interview schedule. Environment did not effect hawkers’ knowledge and attitudes. Education influenced knowledge and attitudes scores of the interviewees. Differences in knowledge of the ethnic groups were in cross-contamination, equipment, utensils and premises, personal hygiene, hazard analysis critical control point (HACCP) and food regulations and control. Malay and Indian hawkers had better educational background, hence better knowledge and attitude scores than Chinese. The findings highlight the importance of education to bridge cultural gaps in food safety knowledge and attitudes. A strong relationship exists between the knowledge and attitudes of hawkers.
Melanocins A, B and C, new melanin synthesis inhibitors produced by Eupenicillium shearii. I. Taxonomy, fermentation, isolation and biological properties.
New melanin synthesis inhibitors, melanocins A, B and C, were isolated from the fermentation broth and mycelium extract of Eupenicillium shearii F80695. Melanocin A, an isocyanide compound, inhibited mushroom tyrosinase and melanin biosynthesis of B16 melanoma cells with IC50 value of 9.0 nM and MIC value of 0.9 microM, respectively. Melanocin A also inhibited growth of Streptomyces bikiniensis. While, the structurally very related but non-isocyanide compounds melanocins B and C did not show inhibitory activity in these assays. Melanocins A, B and C showed potent antioxidant activity with scavenging activity of DPPH radical and superoxide anion radical.