Investigation of cocaine plumes using surface acoustic wave immunoassay sensors.
Vapor sensors, aka electronic noses, are becoming an increasingly popular analytical tool for detection and identification of small molecules in the gas phase. In this paper, we present the results of a series of experiments demonstrating real-time vapor phase detection of cocaine molecules. A distinctive response or signature was observed under laboratory conditions in which the cocaine vapors were presented using an INEL vapor generator and under "field" conditions facilitated by the Georgia Bureau of Investigation (GBI) Crime Lab. For these experiments, the sensor component was a two-port resonator on ST-X quartz with a center frequency of approximately 250 MHz. On this cut of quartz, a temperature-compensated surface acoustic wave is generated via an interdigital transducer. Antibenzoylecgonine (anti-BZE) antibodies are attached to the electrodes on the device surface via a protein-A cross linker. We observed a large transient frequency shift accompanied by baseline shift with the anti-BZE coated sensor. After repeated experiments and the use of numerous controls, we believe that we have achieved real time molecular recognition of cocaine molecules.
Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles

 Abstract
 
 The development and characterization of a quartz crystal microbalance (QCM) sensor for the direct detection of aerosolized influenza A virions is reported. Self-assembled monolayers (SAMs) of mercaptoundecanoic acid (MUA) are formed on QCM gold electrodes to provide a surface amenable for the immobilization of anti-influenza A antibodies using NHS/EDC coupling chemistry. The surface-bound antibody provides a selective and specific sensing interface for the capture of influenza virions. A nebulizer is used to create aerosolized samples and is directly connected to a chamber housing the antibody-modified crystal (“immunochip”). Upon exposure to the aerosolized virus, the interaction between the antibody and virus leads to a dampening of the oscillation frequency of the quartz crystal. The magnitude of frequency change is directly related to virus concentration. Control experiments using aerosols from chicken egg allantoic fluid and an anti-murine antibody based immunosensor confirm that the observed signal originates from specific viral binding on the chip surface. Step-by-step surface modification of MUA assembly, antibody attachment, and antibody–virus interaction are characterized by atomic force microscopy (AFM) imaging analysis. Using the S/N
 =3 principle, the limit of detection is estimated to be 4 virus particles/mL. The high sensitivity and real-time sensing scheme presented here can play an important role in the public health arena by offering a new analytical tool for identifying bio-contaminated areas and assisting in timely patient diagnosis.
 

Coherent pulsed injection seeding of two gain-switched semiconductor lasers
High-power, narrow-spectrum, short pulses at a wavelength near 830 nm are needed for optical logic applications. The authors report the generation of two coherent trains of pulses by pulsed injection seeding of two gain-switched semiconductor lasers with one mode-locked master oscillator. The available power was doubled and both lasers emit in nearly identical spectral lines. Short pulses (30-50 ps) are generated at a repetition rate of 1.9 GHz. The spectrum is reduced to a single mode cluster and shows a 1.7 AA wide chirp suitable for pulse compression. The peak power is 0.1 W for each pulse train. The capability of this technique for coherent power combining is demonstrated.<<ETX>>