A Wear Investigation of Repeatedly Used Wire in Wire Cut Electrical Discharge Machine
Wire electro-discharge machining (WEDM) is a fully extended and competitive machining process widely used for hard and difficult to machine materials. In the present research, experimental investigation have been conducted to find out the wire wear rate at different machining parameter Such as peak current, pulse on time, pulse off time, based on full factorial design of experiment. Molybdenum wire of 0.18 mm and EN-8 material were used as tool and work piece material for the experiment, ANOVA analysis were done to find out the effect of peak current, pulse on time, pulse off time on wire wear and found that the increasing in peak current slowly increase the wire wear but increasing in pulse on time increase the wire wear rapidly and pulse off time not much affects the wire wear. Keywords— WEDM, WWR, ANOVA, Full factorial,
Finite element modeling and optimization to prevent wire breakage in electro-discharge machining
Abstract One of the most important problems in wire electrical discharge machining is related to wire breakage. This research develops a simple finite element model and a new approach to predict the thermal distribution in the wire fairly accurately. The model can be used to optimize the different parameters of the system to prevent wire breakage. At any instant of time, the spatial heat distribution profile of the wire can be mapped on the transient analysis of any point on the wire traversing through all the heat zones from the top spool to the bottom end. Based on this principle, the finite element model and optimization algorithm are used to determine that the heat generated is the critical variable responsible for wire breakage. The model successfully predicts the thermal distribution profile accurately for various wire materials, for increased wire velocity and for reduction in heat transfer coefficient. This simple model is a precursor of development for 3-D finite element models that can describe the cross-sectional wire erosion as the workpiece cutting progresses. The modeling may lead to the development of a smart electro-discharge machining system with a sensor and feedback control to increase the cutting speed and minimize breakage.
Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast.
Abstract The antibiotic cerulenin inhibited the incorporation of 14 C-acetyl-CoA by 67% at a concentration of 9 × 10 −6 M but not that of 14 C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of Saccharomyces cerevisiae . Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity.