Hybridization between Senecio smallii and S. tomentosus (Compositae) on the granitic flatrocks of the Southeastern United States
Senecio smallii andS. tomentosus occasionally hybridize on the granitic flatrocks of the Piedmont of the Southeastern United States. These hybrids are intersectional crosses between sectionsAurei andTomentosi. The parental species have different chromosome numbers withn = 22 inS. smallii andn = 23 inS. tomentosus. Meiosis in the hybrids is abnormal with 22ns and li or 2iis and 3is, and the percentage of stainable pollen is greatly reduced over that of the parents. The hybrid pollen grains are structurally abortive as demonstrated by the scanning electron microscope. Origin of the natural hybrids was documented by the production of artificial hybrids including F1’s, F2’s, and BC’s. Very few of the F2 seeds germinated, and all of the F2 seedlings later died, indicating hybrid breakdown. Seeds of the F1 and BC generations germinated well, and the seedlings were vigorous. Population samples indicate that there is some variation within the natural population systems; however, no evidence of introgression was detected. The isolating mechanisms and possible evolutionary effects of this natural hybridization are discussed.
Cryptic seed heteromorphism in Packera tomentosa (Asteraceae): differences in mass and germination
Seed mass variation and heteromorphism may afford plant species differential germination behavior and ultimately seedling success, particularly in disturbed habitats. We asked whether such variation occurs in Packera tomentosa (Michx.) C. Jeffrey (Asteraceae), a clonal species of the southeastern USA. Seed mass was compared within and among genetic individuals differentiated using amplified fragment length polymorphisms. We compared central and peripheral seeds produced by disc and ray florets, respectively, for their morphology, mass, and germination behavior, including response to water availability, aging, and cold stratification. Seed mass was highly variable both within and among individuals and influenced germination behavior. We found cryptic seed heteromorphism in P. tomentosa. Central and peripheral seeds had similar morphologies but dissimilar mass and biomass allocation. We used failure time analysis to detect different germination behavior. Central seeds were heavier, contained larger embryos, and germinated faster and at a higher proportion in most germination studies. Highly variable mass and heteromorphism of seeds may allow persistence of P. tomentosa in its disturbed habitats. Based on our results, some future studies of Asteraceae species with disc and ray florets may need to account for possible differences between seed types, even when morphological differences are not apparent. Evaluation of individual seed mass and maternal differences in germination studies may assist in the detection of cryptic seed heteromorphism, a phenomenon thought to be common, yet rarely documented.
The Tsetse Problem on the Eastern Cattle Route in the Gold Coast
1. Primary fly-belts round Makongo consisted of three different vegetational communities: a Cynometra-Vitex association along the main length of the Makongo river, on a clay soil; a Ficus congensis association along the river close to the town, on a sandy soil; and Isoberlinia doka woodland on moist sandy soil north-west and south of Makongo. 2. Secondary fly-belts of less dense vegetation occur in the orchard bush savannah round Makongo, and these are not colonised until the commencement of the rainy season. 3. Glossina tachinoides and G. palpalis both occurred in the riverside communities, and G. palpalis alone in the Isoberlinia woodland. 4. Along the river G. palpalis outnumbered G. tachinoides by 3:1 in the dry season. At the commencement of the rains G. tachinoides increased more quickly than G. palpalis , bringing their numbers very nearly equal, but at the end of the rains there was a decrease in G. tachinoides which restored the original proportion. 5. G. morsitans had been found at Makongo formerly but was not met with at the time of this investigation, its absence being due to the disappearance of the larger antelope from the neighbourhood. 6. Smaller game animals, small mammals and reptiles afforded an adequate food supply for tsetse in the fly-belts, and also passing herds of cattle, as well as natives from the village and travellers. 7. The distribution of G. palpalis within the fly-belts was markedly influenced by the proximity of a cattle road along which herds of cattle passed almost daily, and by village water-holes. 8. During the dry season the primary foci were always found to be the places of greatest relative humidity, and the fly does not tend to move out of these until the general atmospheric humidity has reached a certain fairly high degree. 9. In clearing the fly-belts the removal of all primary foci within a quarter of a mile of the town and of thick bush within 100 yards of water-holes was undertaken. The work lasted from the beginning of February to the end of March 1931. 10. The effect of clearing the Isoberlinia doka woodland was to banish G. palpalis completely, and it never returned to these clearings. 11. On clearing the riverside Ficus congensis fly-belt, G. tachinoides was practically eliminated for the first three months, but reappeared in the clearings in June when there was a general increase and up-stream migration of this fly. G. palpalis remained in this clearing in fluctuating numbers up to June, when observations were suspended. 12. 565 marked tsetse were liberated in the fly-belts immediately previous to clearing. Of G. palpalis 12·7% were recaptured in the clearings and 11% outside the clearings. Of G. tachinoides 5·5% were recaptured in the clearings and 6·8% outside.