Theoretical Determination of a Critical Nitrogen Dilution Curve Based on the Carrot Case Study
The objectives of the present study were to: (a) formulate a general dilution equation for a critical nitrogen percentage in a leaf (Nc); (b) monitor dilution function of carrot as a case study; (c) demonstrate applicability of digital color imaging to monitor total nitrogen (Ntot) in crops. Carrot (Daucus carota) was grown on loess soil at five Ntot application rates. Ntot weight (kg/ha) was obtained by standard laboratory analysis and by image processing. Total yield of the treatments was the sum of canopy and roots dry matter. A new logistic decay curve Nc = a/[1 + (W/W0)b] agreed with experimental data. Nc (g/kg) is the critical nitrogen level, a and b are coefficients, and W/W0 is the relative dry matter biomass. Values of Nitrogen Nutrition Index (NNI) ≥ 1.0 indicated that there was no nitrogen deficiency in treatment larger than 100% application of Ntot. The weight of nitrogen in form of multiplication of the dry leaves weight (W) and N percent is suitable for the determination of nitrogen status. The availability of image-based data for N percent is faster, timely and less expensive than that of laboratory test. Applicability of digital color camera to monitor Ntot in crops instead of laboratory test was successfully demonstrated.
Remote estimation of nitrogen and chlorophyll contents in maize at leaf and canopy levels
a b s t r a c t Leaf and canopy nitrogen (N) status relates strongly to leaf and canopy chlorophyll (Chl) content. Remote sensing is a tool that has the potential to assess N content at leaf, plant, field, regional and global scales. In this study, remote sensing techniques were applied to estimate N and Chl contents of irrigated maize (Zea mays L.) fertilized at five N rates. Leaf N and Chl contents were determined using the red-edge chlorophyll index with R2 of 0.74 and 0.94, respectively. Results showed that at the canopy level, Chl and N contents can be accurately retrieved using green and red-edge Chl indices using near infrared (780-800 nm) and either green (540-560 nm) or red-edge (730-750 nm) spectral bands. Spectral bands that were found optimal for Chl and N estimations coincide well with the red-edge band of the MSI sensor onboard the near future Sentinel-2 satellite. The coefficient of determination for the relationships between the red-edge chlorophyll index, simulated in Sentinel-2 bands, and Chl and N content was 0.90 and 0.87, respectively.
Determining a critical nitrogen dilution curve for sugarcane
Adequate measurements of the nitrogen (N) concentration in the aboveground biomass of sugarcane throughout the growth cycle can be obtained using the critical N dilution curve (CNDC) concept, which provides an N-nutrition index (NNI). The aim of this work was to determine the CNDC value for Brazilian sugarcane variety SP81-3250, establish the critical concentration of N, and determine the NNI in the aboveground biomass throughout the cane plant and first ratoon crop cycles. The study was performed in three experimental areas located in Sao Paulo, Brazil, during the crop cycles of 2005/2006 (18-month cane plant) and 2006/2007 (first ratoon). The plant cane crop was fertilized with treatments of 40, 80, or 120 kg N ha–1 and a control treatment without N. After the plant cane harvest, rates of 0, 50, 100, or 150 kg N ha–1 were applied to the control plot and the 120 kg N ha–1–treatment plot in a split-plot experimental design with four repetitions. Throughout both sugarcane cycles, measurements of aboveground biomass were used to determine the dry-mass (DM) production and N concentration for each treatment. CNDC varied between the growth cycles, with a higher N concentration observed in the initial stages of the first ratoon and a lower N dilution observed throughout the plant cane cycle. The NNI value indicated excessive N storage in the initial stages and limiting concentrations at the end of the growth cycle. CNDC and NNI allow for the identification of the N-nutrition variation rate and the period in which the nutrient concentration limits the production of aboveground biomass. The equations for the critical N (Ncr) level obtained in this study for plant cane (Ncr = 19.0 DM–0.369) and ratoons (Ncr = 20.3 DM–0.469) can potentially be used as N-nutritional diagnostic parameters for sugarcane N nutrition.