Untersuchungen zur lichtabhängigen Nitritreduktion von Chlorella
Summary1.The dependence of nitrite reduction activity in Chlorella fuscaShihira et Krauss, as measured by uptake of nitrite, on the developmental stage, light intensity and CO2 concentration was investigated.2.There is a quantitative relation between uptake of nitrite-nitrogen and increase of organic cell-nitrogen.3.The kinetics of nitrite reduction in light are linear with time at high CO2 concentrations (3% in air) but biphasic at low CO2 concentrations (0 to 0.03%). In the steady state phase nitrite uptake is stimulated by an increase in the CO2 concentration from 0 to 0.03%. The initial phase and the steady state phase exhibit a different temperature dependence.4.Nitrite uptake in the steady state phase is completely inhibited by iodoacetamide at concentrations which inhibit the photosynthetic CO2 reduction (5×10−5 M). At lower iodoacetamide concentrations (1 to 2×10−5 M) the linear time course of nitrite uptake at high CO2 concentrations is changed in the same manner as it is when the CO2 concentration is lowered.It is concluded that under special conditions (e.g. low CO2 concentration) the rate of steady state nitrite reduction in light is limited by the supply of carbon skeleton (i.e. N acceptors in the synthesis of organic nitrogen compounds) from the photosynthetic CO2 reduction cycle.5.Pre-illumination in the presence of nitrite followed by a dark period depresses the initial phase of nitrite uptake in light at low CO2 concentrations. Pretreatment with nitrite in the dark lowers the nitrite uptake of the cells in the initial phase by exactly the same amount of nitrite which has been taken up during the pre-darkening period.
 It is concluded that there is a correlation between the initial phase of nitrite reduction in light and the respiration-linked nitrite reduction in the dark.
Nitrate reduction in Chlorococcales
Nitrate reducing activity of ten species of Chlorococcalean green algae, grown in three different nitrogen sources, is reported. Results showed that all the ten species exhibited nitrate reducing capacity but they differed very much in their activity. Induction of nitrate reductase was achieved by incubating in nitrate. Light stimulated the reduction of nitrate in all the ten species.
Assessment of Ground Regeneration around Borehole Heat Exchangers between Heating Seasons in Cold Climates: A Case Study in Bialystok (NE, Poland)
Based on the experimental studies, the process of ground regeneration around the borehole loaded with brine-water heat pumps working exclusively for heating purposes in the period of four consecutive heating seasons in a cold climate was presented. The research was conducted in north-eastern Poland. The aim of the work is to verify the phenomenon of thermal ground regeneration in the period between heating seasons on the basis of the recorded data and to check whether the ground is able to regenerate itself and at what rate. It was noticed that the ground does not fully regenerate, especially during heating seasons with lower temperatures. In the analyzed period, from 22 September 2016 to 12 October 2020, the ground probably cooled irreversibly by 1.5 °C. In order to illustrate and evaluate the speed of changes in the ground, the one’s profile with an undisturbed temperature field was presented for each month of the year. The presented results can be a very important source of information for the analysis of geothermal conditions occurring in the ground. They can be used to verify mathematical models and conduct long-term simulations that allow us to see the complexity of the processes taking place in the ground.