Restoration of pseudo-self-compatibility (PSC) in derivatives of a high-PSC × no-PSC cross in Nemesia strumosa Benth
SummaryMean PSC increased following each generation of recurrent selection in F1, F2 and F3Nemesia strumosa families derived from a cross of a 100% PSC plant to an unrelated 0% PSC plant. The first 100% PSC individuals occurred in the F4. Populations derived through sib pollination tended to have higher PSC means than lines derived through self pollination. One F3 family showed a three-fold higher PSC level when pollinated in the green-house than when pollinated in the growth chamber, while another F3 family similarly pollinated showed no change in PSC.
The inheritance of pseudo-self-compatibility (PSC) in Nemesia strumosa Benth
SummaryPseudo-self-compatibility (PSC) in Nemesia strumosa was determined by recessive modifying genes which interferred with the normal stylar incompatibility reaction. The PSC levels ranged from none or low to amounts resembling full self-compatibility. PSC within a progeny could be maintained at low or high levels by selecting parents either high or low in PSC. Pollen from plants of any PSC level failed to produce seed when placed on other incompatible styles bearing the same S alleles.
Special Stylar Property Required for Compatible Pollen-Tube Growth in Lilium longiflorum Thunb.
Lilium longiflorum (Easter lily) styles capable of supporting compatible pollen-tube growth possess a special property. Compatible pollination of styles lacking this property results in tube growth indistinguishable from that of incompatible pollen tubes in normal styles. This property is not present in L. longiflorum styles aged 6-8 days from anthesis at room temperature or more than 12 days at 4-7 C. It can be removed from Easter lily styles aged at room temperature 5-6 days or at 4-7 C for 7-10 days by immersing them in 50 C water for 6 min; from styles aged 14 days from anthesis at 4-7 C by flushing the stylar canal with 10 drops of distilled water; from styles immersed in 50 C water the day after anthesis by flushing the stylar canal; and from styles aged 1 day from anthesis at room temperature by immersing them in 50 C water for 8 min. The capacity to support compatible pollen-tube growth was not restored in heat-treated (immersed in 50 C water for 6 min), flushed styles by prepollination injection of stigmatic exudate. Similarly, prepollination injection of the material collected by air flushing heat-treated styles had no effect on pollen-tube growth in styles unable to support compatible tube growth because of aging at 4-7 C.