Pinguisane-Type Sesquiterpenes from the South American Liverwort Porella recurva (Taylor) Kuhnemann
The chemical composition of a dichloromethane extract of the South American liverwort Porella recurva has been examined. Two new pinguisane-type norsesquiterpenoid natural products were isolated: 6,11-epoxy-15-nor-3,4-dioxo-5,10-pinguisadien-12-acetate and 6,11- epoxy-15-nor-4-oxo-5,10-pinguisadien-12-acetate. In addition two known pinguisane-type sesquiterpenes were also isolated: norpinguisone and norpinguisone methyl ester. All structures were elucidated by means of NMR spectroscopic techniques and mass spectrometry.
Identification of Volatile Components of Liverwort (Porella cordaeana) Extracts Using GC/MS-SPME and Their Antimicrobial Activity
Chemical constituents of liverwort (Porella cordaeana) extracts have been identified using solid-phase microextraction-gas chromatography mass spectrometry (SPME-GC/MS). The methanol, ethanol and ethyl acetate extracts were rich in terpenoids such as sesquiterpene hydrocarbons (53.12%, 51.68%, 23.16%), and monoterpene hydrocarbons (22.83%, 18.90%, 23.36%), respectively. The dominant compounds in the extracts were β-phellandrene (15.54%, 13.66%, 12.10%) and β-caryophyllene (10.72%, 8.29%, 7.79%, respectively). The antimicrobial activity of the extracts was evaluated against eleven food microorganisms using the microdilution and disc diffusion methods. The minimum inhibitory concentration (MIC) varied from 0.50 to 2.00 mg/mL for yeast strains (Saccharomyces cerevisiae 635, Zygosacharomyces bailii 45, Aerobasidium pullulans L6F, Pichia membranaefaciens OC 71, Pichia membranaefaciens OC 70, Pichia anomala CBS 5759, Pichia anomala DBVPG 3003 and Yarrowia lipolytica RO13), and from 1.00 to 3.00 mg/mL for bacterial strains(Salmonellaenteritidis 155, Escherichia coli 555 and Listeria monocytogenes 56Ly). Methanol extract showed better activity in comparison with ethanol and ethyl acetate extracts. High percentages of monoterpene and sesquiterpene hydrocarbons could be responsible for the better antimicrobial activity.
Taphonomy of Isisfordia duncani specimens from the Lower Cretaceous (upper Albian) portion of the Winton Formation, Isisford, central-west Queensland
Taphonomic analysis of fossil material can benefit from including the results of actualistic decay experiments. This is crucial in determining the autochthony or allochthony of fossils of juvenile and adult Isisfordia duncani, a basal eusuchian from the Lower Cretaceous (upper Albian) distal-fluvial-deltaic lower Winton Formation near Isisford. The taphonomic characteristics of the I. duncani fossils were documented using a combination of traditional taphonomic analysis alongside already published actualistic decay data from juvenile Crocodylus porosus carcasses. We found that the I. duncani holotype, paratypes and referred specimens show little signs of weathering and no signs of abrasion. Disarticulated skeletal elements are often found in close proximity to the rest of the otherwise articulated skeleton. The isolated and disarticulated skeletal elements identified, commonly cranial, maxillary and mandibular elements, are typical of lag deposits. The holotype QM F36211 and paratype QM F34642 were classified as autochthonous, and the remaining I. duncani paratypes and referred specimens are parautochthonous. We propose that I. duncani inhabited upper and lower delta plains near the Eromanga Sea in life. Their carcasses were buried in sediment-laden floodwaters in delta plain overbank and distributary channel deposits. Future studies should refer to I. duncani as a brackish water tolerant species.