Determination of dissolved oxygen by catalytic reduction on Nafion-methyl viologen chemically modified electrode.
A small size Nafion-methyl viologen chemically modified electrode (Nafion-MV CME) together with a small size electrolytic cell were constructed for the purpose of dissolved oxygen (DO) determination. The catalytic reduction of DO on Nafion-MV CME results in fast and sensitive DO determination. The mechanism of such detection is also considered.
Metabolic multianalyte microphysiometry reveals extracellular acidosis is an essential mediator of neuronal preconditioning.
Metabolic adaptation to stress is a crucial yet poorly understood phenomenon, particularly in the central nervous system (CNS). The ability to identify essential metabolic events which predict neuronal fate in response to injury is critical to developing predictive markers of outcome, for interpreting CNS spectroscopic imaging, and for providing a richer understanding of the relevance of clinical indices of stress which are routinely collected. In this work, real-time multianalyte microphysiometry was used to dynamically assess multiple markers of aerobic and anaerobic respiration through simultaneous electrochemical measurement of extracellular glucose, lactate, oxygen, and acid. Pure neuronal cultures and mixed cultures of neurons and glia were compared following a 90 min exposure to aglycemia. This stress was cytotoxic to neurons yet resulted in no appreciable increase in cell death in age-matched mixed cultures. The metabolic profile of the cultures was similar in that aglycemia resulted in decreases in extracellular acidification and lactate release in both pure neurons and mixed cultures. However, oxygen consumption was only diminished in the neuron enriched cultures. The differences became more pronounced when cells were returned to glucose-containing media upon which extracellular acidification and oxygen consumption never returned to baseline in cells fated to die. Taken together, these data suggest that lactate release is not predictive of neuronal survival. Moreover, they reveal a previously unappreciated relationship of astrocytes in maintaining oxygen uptake and a correlation between metabolic recovery of neurons and extracellular acidification.
AN ANALYSIS OF POLYMERIC THICK-FILM RESISTORS AS PRESSURE SENSORS
Abstract The strain-sensing characteristics of thick-film polymer-based resistors printed and cured on 96% alumina are presented. Two layout configurations have been analysed: planar and sandwich. Various strain-gauge characteristics have been studied, including the mechanical and temperature-dependent properties of the polymeric films. It has been shown that the planar polymer resistors exhibit low sensitivity, comparable to that of metal-foil gauges, whereas the MRM (metal-resistor-metal) configurations exhibit similar characteristics to those of cermet thick-film MRM gauges. It has also been observed that the mechanical properties of the polymeric films are significantly poorer than those of cermet-based gauges. This degradation in performance is attributed to the epoxy/pseudo-plastic structure of the polymeric thick-film resistor layer.