EFFECT OF MERTHIOLATE (LILLY) ON CERTAIN SPECIFIC PRECIPITATION REACTIONS.
Merthiolate (Lilly), a substituted benzoic acid, has been shown to interfere, in concentrations usually used for preservation, with the specific precipitation of antisera against beef serum or sheep serum coupled with diazotized p-aminobenzoic acid, p-arsanilic acid, or p-(p-aminophenylazo) phenylarsonic acid. In some systems increased precipitation was observed, while in others decreased precipitation was observed.
The Serological Properties of Simple Substances. IV. Hapten Inhibition of Precipitation of Antibodies and Polyhaptenic Simple Substances
As part of a series of studies of the antigenantibody reaction by use of polyhaptenic simple substances as antigens,we have made a quantitative investigation of the ability of various substituted phenylarsonic acids to inhibit the precipitation of compounds containing two or more phenylarsonic acid groups with antiserum to sheep serum coupled with diazotized p-arsanilic acid. The investigation included study of the effect of various concentrations of hapten XXIII ([chemical structure]) on the amount of precipitate obtained with antiserum and various concentrations of antigen VI [chemical structure], where R is the p-azophenylarsonic acid group); the effect of changed conditions of precipitation for the same system; the effects of four haptens on the precipitation of each of five antigens and each of two pools of antiserum; and the effects of 24 different haptens on one precipitin reaction. The results are discussed in this paper.
Phloroglucinol derivatives from Hypericum japonicum.
The isolation and identification of a new phloroglucinol derivative (2), a diterpenol (4), together with the known compounds flavesone (1) and sarothralen B (3), from the aerial parts of Hypericum japonicum are reported. Their structures were established by extensive spectral analysis and the structure of (3) has also been confirmed by a single crystal X-ray determination.