Time to Radicle Protrusion Does Not Correlate with Early Seedling Growth in Individual Seeds of Impatiens and Petunia
Impatiens (Impatiens wallerana Hooker F.) and petunia (Petunia ·hybrida Hort. Vilm.) seeds were imaged usingaflat-bedscanner interfacedwithapersonal computerprogrammed to captureimages every hour. Imageswere used to measure time to radicle protrusion and seedling growth. Time to radicle protrusion was calculated as time to 50% germination or as actual germination for each seed. Seedling growth after germination was calculated from linear regression of growth over time. Radicle protrusion and seedling growth were evaluated as indicators of seed vigor. Both were good indicators of seed vigor in impatiens seed lots. These measurements of vigor were highly correlated for each impatiens seed lot and for pooled seed lots. However, there was little or no correlation between time to radicle protrusion and seedling growth on an individual seed basis. The relationship between germination speed and seedling growth rate observed in impatiens was confirmed in two petunia seed lots. This study supports the use of time to radicle emergence and seedling growth as good indicators of seed vigor. However, it appears that different aspects of seed vigor may be measured by these indicators because there was no relationship between time to radicle protrusion and seedling growth rate on an individual seed basis.
Genome-wide association mapping unravels the genetic control of seed germination and vigor in Brassica napus
Rapid and uniform seed germination is a crucial prerequisite for crop establishment and high yield levels in crop production. A disclosure of genetic factors contributing to adequate seed vigor would help to further increase yield potential and stability. Here we carried out a genome-wide association study in order to define genomic regions influencing seed germination and early seedling growth in oilseed rape (Brassica napus L.). A population of 248 genetically diverse winter-type B. napus accessions was genotyped with the Brassica 60k SNP Illumina genotyping array. Automated high-throughput in vitro phenotyping provided extensive data for multiple traits related to germination and early vigor, such as germination speed, absolute germination rate and radicle elongation. The data obtained indicate that seed germination and radicle growth are strongly environmentally dependent, but could nevertheless be substantially improved by genomic-based breeding. Conditions during seed production and storage were shown to have a profound effect on seed vigor, and a variable manifestation of seed dormancy appears to contribute to differences in germination performance in B. napus. Several promising positional and functional candidate genes could be identified within the genomic regions associated with germination speed, absolute germination rate, radicle growth and thousand seed weight. These include B. napus orthologs of the Arabidopsis thaliana genes SNOWY COTYLEDON 1 (SCO1), ARABIDOPSIS TWO-COMPONENT RESPONSE REGULATOR (ARR4), and ARGINYL-t-RNA PROTEIN TRANSFERASE 1 (ATE1), which have been shown previously to play a role in seed germination and seedling growth in A. thaliana.
Atmospheric drag effects on the KOMPSAT-1 satellite during geomagnetic superstorms
We examine the atmospheric drag on the low earth-orbiting satellite, KOMPSAT-1 in a sun-synchronous orbit at ~685-km altitude starting in 1999, during a 3-month (October-December) period in 2003. This 3-month interval includes the October 29-30 and November 20 magnetic superstorms and weak to moderate storms. We observed that the daily KOMPSAT-1 drag acceleration transiently responses to transient storm-time disturbances. That is, there is an one-to-one correspondence between the KOMPSAT-1 drag accelerations and the storm events. We find that the drag acceleration correlates strongly with the level of geomagnetic disturbance. This indicates that the trajectory of KOMPSAT-1 is significantly perturbed during extremely disturbed intervals because of atmospheric density increase. The main contributor to the density increase is Joule heating associated with the geomagnetic activity rather than the solar EUV radiation, as reported by previous studies. We suggest that understanding how the upper atmosphere responses to the geomagnetic-associated heating is important to predict space weather impacts on low earth-orbiting satellites.