Acetone as solvent in flame-photometric determination of lithium in serum.
Abstract The effect of some water miscible organic solvents on the flame photometric emission of Li is discussed. A simple procedure is described with acetone as diluting solution giving a ten to twenty fold increase in sensitivity.
Micro flame photometric determination of sodium, potassium, and calcium in serum with organic solvents.
The effect of organic solvents on the flame photometric emission of certain elements in petroleum products has been reported (1). Propanol, hutanol, and acetone have been used in the flame photometric determination of sodium and potassium in serum, but these organic solvents were not used routinely, “as it was feared that complications might arise in practice owing to their volatility and to the restricted solubility of NaCl and KC1 in some solvents” (2). Apparently no extensive investigation has been made of the effect of organic solvents on the emission spectra of the cations of blood serum. We have investigated the use of organic solvents to extend the sensitivity of the flame photometer in the direct determination of sodium, potassium, and calcium, and to make practical the analyses of extremely small biological specimens. Our investigation of the effect of organic solvents on the emission spectra of sodium and potassium (3) indicated that, of the solvents studied, acetone produced the greatest enhancement of emission spectra. Less enhancement was produced by methanol, ethanol, propanol, and isopropanol in the order named. Acetone increased the spectral emission of sodium 17-fold in relative per cent transmittance and potassium 13-fold as compared with the aqueous solvent. This investigation also indicated that bovine albumin* had no effect on sodium and potassium spectra. Further study of organic solvents has been made to test their enhancement effect on the emission spectra of sodium, potassium, and especially of calcium. The use of an aqueous solvent for the direct microphotometric determination of sodium, potassium, and calcium in a single biological specimen has been reported (4).
On the effect of alimentary lipemia and a natural heparinoid on the fibrinolytic system of man.
The effect of alimentary lipemia and Ateroid on the fibrinolytic system of blood was studied in 12 patients. The plasminogen (profibrinolysin), proactivator and antiplasmin activities were determined by the clot lysis time-method.Lipemia had an inhibiting effect on the fibrinolytic system. This appeared most distinctly in the antiplasmin determinations. In the. In the pre-therapeutic fat load tests the changes in the antiplasmin activity differed statistically significantly from those observed during therapy. Ateroid seemed to protect the organism against the antiplasmin effect of lipemia.The female fibrinolytic system seemed to react more sensitively than the male.The effect of Ateroid on the fibrinolytic system seems to be first and foremost a nor-malising influence: it appears most distincly in exceptional circumstances and is very distinct if the divergence is due to hyperlipemia.