Alkaloids ofThalictrum collinum
The method of methanolic extraction was applied to the roots. After the methanol has been distilled off, the concentrated extract was treated with 10% sulfuric acid, the acid extracts were alkalinized to pH 9, and the alkaloids were extracted with ether and chloroform. The combined ether-soluble and chloroform-soluble alkaloids obtained were separated into phenolic and nonphenolic bases. The combined nonphenolic tertiary bases were chromat.graphed on a column of alumina (1:70, neutral, activity grade II). The alkaloids were eluted with benzene, benzene--chloroform, chloroform, ethyl acetate, and ethyl acetate-methanol.
New Flavonoid Glycoside from Thalictrum minus
The new flavonoid diglycoside thamiflaside was isolated from the aerial part of Thalictrum minus. The chemical structure of this glycoside was determined as apigenin 7-O-α-L-2″′-methoxyrhamnopyranosyl-(1→6)-β-D-glucopyranoside based on PMR and 13C NMR (DEPT, HSQC, HMBC) physicochemical methods.
A convenient separation of alkaloid mixtures by partition chromatography, using an indicator in the stationary phase.
Abstract A convenient method has been developed for the separation of the alkaloids of Buxus sempervirens L. This method involves the use of partition chromatography on Kieselguhr, with an indicator in the stationary phase; the separate bands are detected visually, collected as a whole and in many cases crystallized directly. General considerations are presented concerned with the application of this method to other alkaloidal mixtures. The method is shown to give rapid and convenient separations of mixtures of bisbenzylisoquinoline alkaloids; of complex mixtures of indole alkaloids; and of mixtures of veratrum ester alkaloids, including an efficient and complete separation of a natural mixture of cevadine and veratridine.