Effect of electric impulse on sporocarp formation of ectomycorrhizal fungusLaccaria laccata in Japanese red pine plantation
We examined the effect of electric impulses on sporocarp formations of ectomycorrhizal fungus in a Japanese red pine (Pinus densiflora) plantation.Laccaria laccata was the dominant species in this study plantation. Spatial distribution concentrated in the electric impulse treatment zone, and recorded a markedly larger number of sporocarps compared with untreated control plots. The effect of electric impulses was also recognized onL. laccata fruiting with a mycorrhizal sapling in a pot. Sporocarp production ofL. laccata was strongly promoted by the electric impulse in experiments bothin vivo natural plantation stands andin vitro pot saplings. Our work indicates the possibility of enhancement of sporocarp production of the mycorrhizal fungi with electric impulses in the nursery and plantation.
Development of Compact High-Voltage Power Supply for Stimulation to Promote Fruiting Body Formation in Mushroom Cultivation
The compact high-voltage power supply is developed for stimulation to promote fruiting body formation in cultivating L. edodes and Lyophyllum deeastes Sing. mushrooms. A Cockcroft-Walton (C-W) circuit is employed to generate DC high-voltage from AC 100 V plug power for the compact, easy handling and high safety use in the hilly and mountainous area. The C-W circuit is connected to high-voltage coaxial cable which works for high-voltage transmission and for charging up as energy storage capacitor. The output voltage is around 50 kV with several microseconds pulse width. The dimension and weight of the developed power supply are 0.4 × 0.47 × 1 m3 and 8.1 kg, respectively. The effect of the high-voltage stimulation on enhancement of fruiting body formation is evaluated in cultivating L. edodes and Lyophyllum deeastes Sing. mushrooms using the developed compact high-voltage power supply. The conventional Marx generator is also used for comparison in effect of high-voltage stimulation for fruiting body formation. L. edodes is cultivated with hosting to natural logs and the pulsed high voltage is applied to the cultivated natural logs. The substrate for Lyophyllum deeastes Sing. cultivation consists of sawdust. The results show that the fruiting body formation of mushrooms of L. edodes for four cultivation seasons and that of Lyophyllum deeastes Sing. for two seasons both increase approximately 1.3 times higher than control group in terms of the total weight. Although the input energy per a pulse is difference with the generators, the improvement of the fruit body yield mainly depends on the total input energy into the log. The effect for promotion on fruiting body formation by the developed compact high-voltage power supply is almost same that by the conventional Marx generator.
Pinus resinosa Ectomycorrhizae: Seven Host-Fungus Combinations Synthesized in Pure Culture
Results of pure culture mycorrhizae synthesis tests made with rect pine (Pinus resinosa) and ten known or suspected mycorrhizal fungus isolates native to the northern Great Lakes states