Analysis of Clostridium botulinum toxigenic types A, B, and E for fatty and carbohydrate content.
Lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic KOH. Gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of Clostridium botulinum toxigenic types "A," "B," and "E." C. perfringens type "A" and Escherichia coli strain "B" were also studied in a similar manner and were found to give pattern profiles which were distinct even from those obtained for the C. botulinum microorganisms. Amino sugar content of the five microorganisms was determined by using a Beckman amino acid analyzer. The molar ratio of glucosamine to that of galactosamine was found to be of further assistance in distinguishing the individual microorganisms.
Gas chromatography of bacterial whole cell methanolysates; V. Fatty acid composition of Neisseriae and Moraxellae.
Twentyfive strains or substrains belonging to the bacterial species Moraxella nonliquefaciens, M. bovis, M. kingii, M. osloensis, M. phenylpyrouvica and M. urethralis (tentative designation) were examined by gas-liquid chromatography (GLC) of whole cell methanolysates. Each strain gave a reproducible chromatogram, usually with no difference between non-fimbriated and fimbriated variants. With a few exceptions, there was no significant difference between the elution profiles of strains belonging to the same species. The study revealed similarities as well as distinct dissimilarities in interspecies comparisons. When compared with M. bovis, the species M. nonliquefaciens and M. phenylpyrouvica showed most similarity, whereas M. kingii revealed the greatest chromatographic deviation. The remaining two species were intermediate in this respect. The results are discussed in relation to taxonomic data previously arrived at by other methods, in particular measurements of nucleic acid homologies and heterologies. Generally, the two groups of results were consistent, and it is concluded that GLC is a valuable tool for evaluation of relationship between species, adding significantly to the reliability of species allocation to genus. GLC appears also applicable for species identification of isolates of moraxellae and similar organisms. However, the sometimes great similarities between closely related species and the occasionally observed (minor) intraspecies variations in elution profile substantiate the need for supplementary methods.
Population and Settlement
Part 1 Population distribution: world population distribution variations in world population distribution population distribution in the Highlands and Islands population distribution in Japan population distribution in Brazil. Part 2 Population growth and movement: population structure the world population explosion variations in population growth migration why do people migrate types of migration in North and West Africa West German migration populating the USSR populating Australia attitudes to population control population and natural resources. Part 3 Settlements: location of settlement settlement types settlement functions settlement patterns South Wales valley settlements the changing village. Part 4 Urbanization: million cities world urbanization rural-urban migration in the developing world urban land use inner city problems urban transport the Tyneside metro spheres of influence changing shopping habits the Dutch Randstad Lagos - a city in the developing world new and expanding towns in the UK new settlements in developing countries green belts or red bricks? Part 5 Further studies: UK population migration Kenya's population problems Sheffield Washington New Town.