Characterization of series arcs in LVdc microgrids
This paper provides an empirical study on series arc behavior in low voltage dc microgrids. The response of an R-L-C dc microgrid abstraction towards series arcs is studied experimentally for varying grid inductance, dc voltages, load capacitances and load currents. In order to account for the stochastic nature of arcs, experiments are repeated multiple times under similar conditions to gain statistical significance. Thereby, insight on percentage occurrence and burn time of initiated series arcs is provided. Load side voltage response is studied to gain insight on the expected peak drop and fall time. This empirical evidence was judged to be a necessary requirement in developing a novel series arc extinguishing method from load side power electronic devices.
DC microgrid islands on ships
Since shipboard power systems are inherently islanded and consist of energy intensive sources and loads, it is an interesting application for dc microgrids. The object of this paper is to explore the merits of dc based shipboard power distribution as compared to conventional ac solutions. A survey of current initiatives, emerging concepts and design challenges is carried out. Based on the review, insight is offered on different aspects like efficiency, safety, and reliability and their relevant tradeoffs. It will be discussed how the interplay of reliability and availability, driven by the computational cost of optimization and restoration goals, forms the heart of reconfigurability.
Overproduction of a truncated poly (vinyl alcohol) dehydrogenase in recombinant Pichia pastoris by low-temperature induction strategy and related mechanism analysis
Based on the N-terminal sequencing of poly (vinyl alcohol) dehydrogenase (PVADH), a 1,644-bp gene encoding a truncated PVADH (tPVADH) was amplified using the synthetic gene (GenBank accession No. JQ235753) as a template, and was further transformed into Pichia pastoris GS115 with the vector pPIC9K. The maximal tPVADH activity reached 546 U/mL in shake flask. The influence of methanol concentration and induction temperature on tPVADH production was further investigated in 3-L bioreactor. When the methanol concentration and induction temperature were controlled at 15 g/L and 22 °C, respectively, the maximal tPVADH activity reached 8,464 U/mL, which was nearly 10 times that of mature PVADH expressed under the same condition and was the highest level ever reported. The reason responsible for the significant improvement of tPVADH production at low induction temperature was explored in terms of cell viability, extracellular proteases activity, and alcohol oxidase activity.