Spine skeleton morphogenesis during regeneration in clypeasteroid and camarodont sea urchins
The process of skeleton morphogenesis is described for broken and totally removed spines in clypeasteroid (hollow spine) and camarodont (solid spine) sea urchins. Spine regeneration after total spine removal is completed in 40–45 days in clypeasteroids and in 60–70 days in camarodont sea urchins. Along with common stages of formation of longitudinal ribs in both hollow and solid spines, fundamental differences were found between the initial stages of reparative growth of the spine shaft. The spine shaft is formed from a single median process in clypeasteroids and from many simultaneously growing processes in camarodont sea urchins. Reparative morphogenesis of totally removed and partly broken spines in clypeasteroid sea urchins and totally removed spines in camarodont sea urchins leads to the formation of a skeletal structure identical to the intact spine. However, during the regeneration of broken camarodont spines, lateral growth is markedly retarded. As a result, the regenerated part of the spine shaft has a smaller diameter when the initial spine length is achieved. A hypothesis is proposed on a paedomorphic origin of spines in the clypeasteroid sea urchins on the basis of the juvenile stage of definitive spines in the camarodont sea urchins.
Expression of SM30(A–F) genes encoding spicule matrix proteins in intact and damaged sea urchin Strongylocentrotus intermedius at the six-armed pluteus
In this study we investigated expression of the SM30(A–F) gene family encoding Strongylocentrotus intermedius spicule matrix proteins during the normal and regenerative pluteus II stage (three pairs of arms). We found that SiSM30A and SiSM30B genes are expressed at high levels in the normal pluteus II sea urchin. SiSM30A is expression was also significantly upregulated in the reparative pluteus II stage 3 hours after damage. Conversely, SiSM30B was downregulated during the reparative pluteus II stage. Our findings reveal a substantial similarity between the activity of SiSM30A and SiSM30B activity in the processes of regenerative growth during the pluteus II stage and during normal development at the prism stage in Strongylocentrotus purpuratus. On the basis of our findings, we propose that normal developmental mechanisms corresponding to the preceding developmental stage are reactivated during pluteus regeneration.
Frequency distributions of the mixing height over an urban area from SODAR data
The height of the mixing-layer (MLH) is an important parameter in the assessment of the dilution of primarily emitted or secondarily formed air pollutants in the atmospheric boundary-layer. A continuous measurement of MLH is possible only by remote sensing. Here, 17 months of SODAR data have been analysed automatically to derive the MLH over the city of Hannover in Northern Germany. In contrast to earlier studies the MLH has been determined from vertical profiles of the acoustic backscatter intensity and from the variance of the vertical velocity component. The results are presented in form of monthly frequency distributions and mean daily courses of MLH. These statistical evaluations of MLH show a clear annual course and interannual variability. The study shows the possibility to derive meaningful climatological information from long-term SODAR measurements for air quality issues.