A radioimmunoassay combined with solid-phase extraction for the determination (pg ml-1) of AR-C15849XX) in human plasma.
A radioimmunoassay has been developed for the determination of AR-C15849KF, a CCK-8 analogue, in human plasma. The method incorporates solid-phase sample extraction, is suitable for the determination of the analyte at pg ml-1 concentrations and is based on a method developed and validated for dog plasma. The solid-phase extraction, using ion-exchange aminopropyl and octadecyl sorbents sequentially, was retained for this procedure to remove matrix interferences in the plasma and to enhance method sensitivity. The calibration range is 10-500 pg ml-1, using a 1 ml sample of undiluted human plasma. The method has been successfully used to generate early human pharmacokinetic data during a programme of exploratory development.
A radioimmunoassay combined with solid-phase extraction for the determination of a novel anti-obesity agent, ARL 15849XX, in dog plasma.
A radioimmunoassay has been developed for the determination of ARL 15849XX, a cholecystokinin-8 (CCK-8) analogue, in dog plasma. The method incorporates solid-phase sample extraction and is suitable for the determination of the analyte at picogram per millilitre concentrations. The antiserum was raised in Suffolk-cross sheep following primary and booster immunisations with an immunogen prepared by conjugating ARL 16935XX, an analogue of ARL 15849KF, to bovine serum albumin. The radioligand was prepared by the no-carrier-added 125I iodination of a non-sulphated derivative, ARL 15745XX. The solid-phase extraction procedure, carried out using ion-exchange aminopropyl and octadecyl sorbents sequentially, was introduced to remove matrix interferences in the plasma and to enhance the method sensitivity. The calibration range is 20-1000 pg ml-1, using a 1 ml sample of undiluted dog plasma.
Study on Electrical Explosion Mechanism and Ignition Characteristics of Copper and Aluminum Powder Under Ignition Solid Propellant
In order to study the characteristic of the electrical explosion of copper and aluminum powders, a multilayer generator was designed, an experimental system was set up, and the parameters of metal powder electrical explosion were measured and analyzed. The results show that the duration of the electrical explosion process increases significantly due to the addition of metal powder. The duration of aluminum powder electrical explosion jet flow is ~86.36 ms due to its self-sustained combustion, which releases a large amount of energy, and a small fraction of aluminum powder particles is not burned. The duration of copper powder electrical explosion jet flow is ~15.84 ms, and most of the copper powder particles are in the solid state. The current pause process is absent; instead, the first discharge process is connected to the second discharge process. The effect of copper powder on the first discharge process is greater than that of aluminum powder. Furthermore, the ignition ability of copper powder electrical explosion to solid propellant is stronger than that of aluminum powder in a closed bomb experiment. These results are indubitably meaningful for the application of metal powder electrical explosion and ignition of solid propellant in an electrothermal chemical gun.