An elegant synthesis of Zetaclausenamide.
Zetaclausenamide, which was isolated as a hepatoprotective agent from the leaves of medicinal plant Clausena lansium, was synthesized for the first time in six steps including Darzen's condensation, photoisomerization, and the final cyclization reactions.
The Fruits of Wampee Inhibit H2O2-Induced Apoptosis in PC12 Cells via the NF-κB Pathway and Regulation of Cellular Redox Status
Wampee (Clausena lansium) fruits (CLS), whose pulp can be used to prepare fruit cups, desserts, jam, or jelly, can be eaten along with the peel. In this study, a PC12 cell model was built to observe the protective effect of CLS against H2O2-induced oxidative stress. We found that pretreatment with CLS increased cell viability and inhibited cytotoxicity, caspase-3 activity and DNA condensation. CLS also attenuated the increase in ROS production and MMP reduction. Moreover, we attempted to determine whether CLS suppressed the expression and phosphorylation of NF-κB. Western blot and immunostaining assay revealed that CLS inhibited H2O2-induced up-regulation of NF-κB p65 and pNF-κB p65. And CLS significantly suppressed the translocation of NF-κB p65 and pNF-κB p65 from cytoplasm to nuclear. Also, seven major compounds including a new flavanoid, luteolin-4'-O-β-d-gluco-pyranoside (3) and six known compounds 1,2, 4–7 were isolated and identified from CLS. Their antioxidative and H2O2-induced PC12 cell apoptosis-reversing activity were determined. These findings suggest that CLS and its major constituents (flavanoids) may be potential antioxidant agents and should encourage further research into their use as a functional food for neurodegenerative diseases.
Chemical-Looping Combustion of Biomass in a 10 kWth Reactor with Iron Oxide As an Oxygen Carrier
Chemical-looping combustion of biomass was carried out in a 10 kWth reactor with iron oxide as an oxygen carrier. A total 30 h of test was achieved with the same batch of iron oxide oxygen carrier. The effect of the fuel reactor temperature on gas composition of the fuel reactor and the air reactor, the proportion of biomass carbon reacting in the fuel reactor, and the conversion of biomass carbon to CO2 in the fuel reactor was experimentally investigated. The results showed that the CO production from biomass gasification with CO2 was more temperature dependent than the CO oxidation with iron oxide in the fuel reactor, and an increase in the fuel reactor temperature produced a higher increase for the CO production from biomass gasification than for the oxidation of CO by iron oxide. Although the conversion of biomass carbon to CO2 in the fuel reactor decreased with the increase of the fuel reactor temperature, there was a substantial increase in the proportion of biomass carbon reacting in the fuel react...