Haemolysis Effect Estimation of Doxycycline Hyclate 150 mgDelay Release Tablet in Bio-analysis (Human Plasma) by LiquidChromatography-Tandem Mass Spectrometry
A fast, simple and particular method for estimation of Doxycycline in healthy human plasma was validated using Minocycline as IS. The analyte and IS were extracted from plasma using SPE. The compound was estranged on a RP column with an isocratic mobile phase consisting of 0.1% formic acid in water and acetonitrile (12:88, v/v), and detected by tandem mass spectrometry in positive ion mode. The ion transition recorded in several reaction monitoring mode were m/z 294.1→225.1 for Doxycycline and m/z 286.1→217.1 for IS. Linearity in plasma was observed over the concentration range 0.3-30 ng/mL for Doxycycline. The mean recovery for Doxycycline was 83.7%, with a lower limit of quantification of 0.3 ng/mL. The coefficient of variation of the assay was less than 6.8%, and accuracy of 96.1% to 102.2%. The validated method was applied to bioequivalence study of 150 mg Doxycycline Hyclate tablet in healthy human volunteers. The validated method was used to expose study samples of bioequivalence study of 150 mg Doxycycline Hyclate delay release tablet in 36 healthy human volunteers. Total 50 samples from individual volunteers identified as Haemolyzed, which were analyze initial and repeat again to cross check the method reproducibity for Haeamolysis effect and compared which found acceptable range. Bioequivalence was prove for test and reference using validated method to experimental samples (Figure 1).
Solid dosage forms: Tablets
Oral drug delivery is most commonly route of administration, when compared to all other route of administration. Oral route is most convenient, safe route of administration. Solid medicaments may be administered orally like tablets, capsules, pills, powders etc. One of the solid dosage form administered orally is tablet. Tablets are solid dosage form containing medicaments with or without excipients. Tablets are prepared by compressing a drug with or without diluents.
Optical rotatory dispersion and absolute configuration—III
Abstract Optical rotatory dispersion measurements on pyrrolidine, piperidine, and tetrahydro-isoquinoline alkaloids indicate that the absolute configuration of the α-asymmetric center may be assigned directly from the dispersion curve, provided either that the nitrogen atom is not protonated, or that the compound contains a chromophore absorbing above 200 mμ.