Attributes related to seasonal herbage growth in white clover
When breeding temperate forage species is investigated, some attributes such as herbage accumulation and seasonal growth patterns have to be considered. To modify some of these traits, knowledge of the detailed process might be useful. In order to evaluate seasonal growth of contrasting white clover populations an experiment was carried out. Treatments were five cultivars and three local populations collected in Argentina. Weekly measures were taken during each season to calculate leaf appearance and flower appearance rates, stolon growing rate and dry matter (DM) net accumulation. Different germplasm of this species have different mechanisms for DM accumulation. Leaf size, more than leaf appearance rate, was the variable with most differences among germplasm and mostly related to dry matter accumulation. During spring and summer, inflorescences production had important effects on growing stolon rate, and differences among germplasm were evident. Some local populations showed favourable attributes that could be useful for breeding.
ISOLATION OF CYTOKININ BIOSYNTHESIS AND METABOLIC GENES FROM WHITE CLOVER (Trifolium repens L)
The factors influencing senescence in white clover (Trifolium repens L.) are of considerable importance to the pastoral sector of New Zealand’s economy. The plant hormones, ethylene and the cytokinins, have been implicated as having opposing influences on senescence. This project focused on the cytokinins. The rate limiting step in cytokinin biosynthesis is catalysed by isopentenyl transferase (IPT) and the primary enzyme in the degradation of cytokinins is cytokinin oxidase/dehydrogenase (CKX). Both IPT and CKX genes are present as multi-gene families. A reduction in the level of active cytokinins either via a decrease in IPT expression, or an increase in CKX expression, or both, would implicate the cytokinins in developmental leaf senescence in white clover. White clover grows in a sequential pattern with leaves at all stages of development making it a good model for studying leaf development and senescence. A decrease in leaf chlorophyll is used as a marker for the onset of senescence. A micro-scale chlorophyll analysis was developed using the NanoDropTM thus allowing tissue from the same leaflet to be used for gene expression and chlorophyll measurements. The pattern of chlorophyll changes was similar to that shown by Hunter et al.(1999) and Yoo et al.(2003) in white clover stolons used for ethylene research. Reverse transcriptase PCR (RT-PCR) and BLAST analysis was used to identify five putative IPT genes and seven putative CKX genes from white clover. RT-PCR demonstrated the expression of seven of these genes (TrIPT1. TrIPT13, TrIPT15 TrCKX1, TrCKX2, TrCKX6). Analysis with quantitative real-time PCR showed expression of TrCKX2 increased markedly during leaf expansion and was consistently high during senescence, suggesting a potential role for CKX in facilitating the progression of senescence.
The Determination of Physicochemical Characteristics of Biodiesel and Its Blending Oils
The viscosity,combustion heat,sulfur content,carbon residue,ash,flash point,moisture and cetane value of biodiesel and its blending oils with diesel were determined.The influence of temperature on the viscosity was studied,and the physicochemical characteristics of biodiesel and its blending oils were compared with diesel.The results indicated that biodiesel and its blending oils had some superior performances and good application prospect.