Seed coat permeability and its inheritance in a forage lupin (Lupinus hispanicus)
SummaryThe species Lupinus hispanicus, throught its use as self-seeding forage, can contribute to solve the problem of forage scarcity during the summer in the livestock farming of the central-west Iberian Peninsula. A feature of the highest importance in achieving self-seeding is the seed-coat permeability. An evaluation of this characteristic has been carried out in a Lupinus hispanicus collection from Salamanca (Spain), its inheritance, and the influence of daily temperature fluctuations on the ‘softening’ of the seeds. All the lines but one had impermeable seeds. It was established that the permeability of the seeds is controlled by the genotype of the mother-plant and the inheritance of this characteristic follows a simple Mendelian model, the permeability being recessive. It was also observed that seeds subjected to daily temperature fluctuations had an increased level of permeability.
The relationship between temperature fluctuations and the softening of hard seeds of some legume species
Hard seeds of subterranean clover (Trifolium subterraneum L.) of the Geraldton and Bacchus Marsh strains, and of West Australian blue lupin (Lupinus varius L.), were subjected to various daily fluctuating temperatures within the normal summer environmental range (15–75°C). The main factor determining the rate of softening of the hard seeds was the maxinlum temperature of the fluctuation. Provided the temperature changed by some 15°C , the amplitude of the fluctuation did not appear to be a critical factor. The softening of hard seeds of any particular species did not commence until the amplitude of the temperature fluctuation, or the maximum temperature, reached a certain level, which in turn varied with the species. Beyond this level the rate of softening increased with increasing fluctuations to a point where the rate became very rapid, and thereafter wider fluctuations or higher maximum temperatures did not give significant increases.
Instrumentation and Automated Photometric Titration Procedure for Total Acidity Determination in Red Wine Employing a Multicommuted Flow System
An automated procedure for photometric titration of red wine and associated instrumentation is described. The procedure was based on the flow-batch approach implemented employing multicommutation. The photometric detection was carried out using a homemade LED-based photometer. The mixing device, LED, and photodetector were attached to the titration chamber in order to form a compact and small-sized unit. The flow system comprised an automatic injector and three-way solenoid valves, which were controlled by a microcomputer through an electronic interface card. The software, written in Quick BASIC 4.5, was designed with abilities to accomplish all steps of the titration procedure including data acquisition and real-time processing to decide about the course of titration in the following step and so forth, until the titration endpoint was reached. The usefulness of the proposed titration system was demonstrated by analyzing red wine samples. When results were compared with those obtained using the AOAC reference method, no significant difference was observed at the 95% confidence level. A relative standard deviation of ca  2% (n=9 ) was obtained when processing a typical red wine sample containing 7.3 gl -1 total acidity expressed as tartaric acid.