A study of the reaction of pentacyclic triterpenoids with concentrated sulfuric acid
SummaryThe conditions for the reaction of triterpenoids with concentrated sulfuric acid have been studied by the method of mathematical planning, and the optimum conditions for performing this reaction have been found.
Zur spektralphotometrischen Bestimmung von steroiden und triterpenoiden Verbindungen
Sterole, Steroide, Steroidsaponine, Triterpensaponine und deren Aglykone weisen bei der spektralphotometrischen Messung in konzentrierter Schwefelsäure im UV‐Bereich eine Absorptionsbande auf, die zur quantitativen Bestimmung der genannten Verbindungen verwendet werden kann. Die Messung kann mit den freien oder an ein DC‐Sorptionsmittel adsorbierten Verbindungen durchgeführt werden. Die Schwefelsäureumsetzungsprodukte wurden isoliert und analysiert, jedoch konnte der Reaktionsmechanismus noch nicht endgültig aufgeklärt werden.
Liebermann-Burchard Reaction for Steroids
THE Liebermann–Burchard reaction for steroids1, which consists in adding a few drops of acetic anhydride and concentrated sulphuric acid to the chloroform solution of the sterol, is not only used as a qualitative test for it but also for its quantitative estimation in blood, etc. Formation of coloured halochromic sulphate of ketone was suggested by Wieland and Weil2 in explaining this reaction. The reaction product, when poured into water, was found to be soluble in the latter, and the grass-green coloration was observed. By studying this water-soluble colouring product, it has been possible for us to state definitely that it is the sulphonic acid derivative of cholesterilene, which has previously been reported to be formed through the action of sulphuric acid on cholesterol dissolved in glacial acetic acid3. The acid gives insoluble calcium and barium salts.