Thermochromatism and structural evolution of metastable polydiacetylenic crystals.
Topochemically polymerized sodium 10,12-pentacosadiynoate (PCDA-Na) microcrystals show an irreversible red-to-blue chromatic transition accompanied by a distinct structural evolution upon initial thermal treatment, and show a subsequent completely reversible blue-to-red chromatic transition upon further thermal stimuli. Visible absorption spectroscopy, X-ray diffraction (XRD), and differential scanning calorimetry (DSC) are used to investigate the thermochromatic transition behavior of the polydiacetylenic microcrystals. Brief quantum mechanical geometry optimization is employed to explain the lattice dimensional change during the irreversible red-to-blue chromatic transition of the metastable polydiacetylenic crystals.
Functionalisation of silica-carbonate biomorphs.
Biomorphs are a unique class of self-organised silica-carbonate mineral structures with elaborate shapes. Here we report first approaches to modify these complex inorganic architectures through silane chemistry, binding of nanoparticles, and organic polymerisation. This leads to functional nanostructures in which the complexity of the originally inorganic template is preserved, and offers new diagnostic tools to study the mechanisms underlying their formation.
Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. II. Alterations of in vitro lymphoproliferation and in vivo immune responses.
To determine the functional impact of alterations in lymphocyte concentrations and ratios following infection with chicken anemia agent (CAA) alone or in combination with infectious bursal disease virus (IBDV) on the immune system of young chickens, in vitro lymphoproliferation assays and in vivo responses to vaccination with several common viral agents were assessed at various time intervals post-inoculation (PI). Concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed mitogen (PWM) stimulation of splenic lymphocytes (SPL) collected from control birds could not be detected until 10-14 days PI. Infection with CAA was characterized by significantly higher PWM stimulation of SPL at 17 days PI and significantly lower PWM stimulation of peripheral blood lymphocytes (PBL) at 14 days PI, compared with uninfected controls. Concanavalin A and PWM stimulation of SPL was significantly increased in birds inoculated with IBDV alone. Lymphocytes harvested from birds inoculated simultaneously with CAA and IBDV had significantly lower responses. Effects on humoral and cell-mediated immunity following CAA and/or IBDV were determined by evaluating vaccination responses to Newcastle disease virus (NDV), fowl pox virus (FPV) and infectious laryngotracheitis virus (ILTV) during the acute phase of CAA infection (2 weeks PI). Vaccination of birds 2 weeks following CAA infection at 1 day of age resulted in decreased protection against NDV (85.7%) and ILTV (7.1%) challenge compared with protection rates in control birds (100% and 53.3% respectively). Infectious bursal disease virus infection was associated with decreased protection against NDV (60%) only. Concomitant infection at 1 day of age resulted in a greater reduction in NDV challenge protection (33.3%), slightly decreased FPV protection (87.5%), increased numbers of persistent FPV vaccination lesions and increased protection against ILTV challenge (71.4%). Vaccination of birds 2 weeks following CAA infection at 2 weeks of age resulted in slightly decreased NDV humoral antibody, development of persistent FPV vaccination lesions (17%) and increased immunity to ILTV challenge compared with control birds (83.3% vs. 66.7%). Chickens inoculated with IBDV alone displayed a more severe depression in NDV antibody titers and only a slight decrease in ILTV protection. Vaccination following concomitant infection at 2 weeks of age resulted in a higher percentage of FPV persistent vaccination lesions (39%) and greatly enhanced immunity to ILTV challenge (100%).