NEAR INFRA RED SPECTROSCOPIC ANALYSIS OF MACROTYLOMA UNIFLORUM ( LAM . ) VERDC . , PHASEOLUS LUNATUS LINN . AND PHASEOLUS VULGARIS
The present study is designed to determine the types of secondary metabolites in three selected seed powders by fast, reliable, and non-destructive Near Infra Red spectroscopic analytical technique. NIRS analysis of Macrotyloma uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., and Phaseolus vulgaris Linn., seed flours were done for qualitative evaluation of secondary metabolites. The fine powder of crude drug was directly applied in FOSS XDS near infrared Rapid Content Analyzer. The properly filled powdered sample in a disposable glass silicate vial and closed with plastic lid was centrally placed onto the sample presentation glass of Foss Rapid Content Analyzer. The scanning of sample at ambient temperature gives NIR spectra within 30 seconds. The major proposed compounds in M. uniflorum were 3,4-dihydroxybenzoic acid, caffeic acid, chlorogenic acid, ferulic acid, gallic acid, kaempferol, linolenic acid, niacin, quercetin, sinapinic acid, syringic acid, and vanillic acid. Whereas, coumaric acid, fructose, galactose, L-serine, raffinose, stachyose, sucrose, threonine, and tyrosine were detected in P. lunatus and the NIR suggested the presence of caffeic acid, ferulic acid, sinapinic acid, phytic acid, leucine, and valine in P. vulgaris. NIRS as an advance innovative technology extensively used in chemical, food, petrochemical, and pharmaceutical industries for rapid and reliable standardization. The present study revealed a good correlation to conclude that flour samples of all the three legume seeds could potentially be added to food system to achieve nutritional and functional benefits.
Protein and mineral content of a novel collection of wild and weedy common bean (Phaseolus vulgaris L)
Protein and total calcium, iron, and zinc were determined in 70 accessions of wild and weedy common bean (Phaseolus vulgaris L) from different sites in two Mexican states (Jalisco and Durango). Protein digestibility, essential amino acid profiles, tannins, phytic acid and extractable iron were determined in selected accessions. The phytate/zinc and the phytate (calcium/zinc) molar ratios were also determined as predictors of zinc bioavailability. For comparative purposes, two cultivated common beans were included. The wild and weedy beans contained more protein and similar protein digestibility compared with cultivated samples. The contents of sulfur amino acids were low in all samples; additionally, beans from Jalisco had higher contents of sulfur amino acids than cultivated ones. Beans from Durango showed higher leucine, valine and aromatic amino acids contents than cultivated beans. Some wild and weedy beans from Jalisco and Durango showed high contents of calcium (7470 mg kg ˇ1 ), iron (280 mg kg ˇ1 ), and zinc (33.1 mg kg ˇ1 ). The phytic acid (calcium/zinc) molar ratios of some wild and weedy beans were similar to those of cultivated beans. Amounts of extractable iron were in the order of 26-74%. # 2000 Society of Chemical Industry
Anthocyanins of Phaseolus vulgaris, cultiva. Kurodanekinugasa
(1) クロダネキヌガサインゲンの種皮の色素はペーパークロマト法により4種類存在することを認めた。(2) 種皮を0.5%塩酸-メタノールで抽出後, アンバライトCG-50, セルロースパウダーによるカラムクロマト法, マスペーパークロマト法により4種の色素を単離した。(3) 得られた各々の結晶のうち, 色素B, C, Dはミクロツアイゼル法によりメトキシル基を検出した。また加水分解の結果, 結合糖はいずれもグルコースであり, 色素Aのアグリコンはデルフィニジン, 色素Bのアグリコンはペツニジン, 色素C, Dのアグリコンはマルビジンであった。(4) 吸収スペクトル, 部分加水分解等により, 色素Aはデルフィニジン-3-モノグルコサイド, 色素Bはペツニジン-3-モノグルコサイド, 色素Cはマルビジン-3-モノグルコサイド, 色素Dはマルビジン-3, 5-ジグルコサイドと同定した。