GC-MS ANALYSIS AND ANTICANCER ACTIVITY OF METHANOL EXTRACT OF LEAVES OF HYPERICUM HOOKERIANUM WIGHT & ARN
Objective: The present investigation was an attempt to study the anticancer activity of the methanol extract of the leaves of H. hookerianum. GC-MS analysis revealed that the methanol extract of leaves contained important secondary metabolites viz. oxygenated hydrocarbons and phenolic hydrocarbons. These phytochemicals w e r e p r e s u m e d responsible for various pharmacological actions like antioxidant, and anticancer activities. Cytotoxic changes observed in MCF 7 cell line by the treatment of methanol extract included cell aggregation, cell rounding and cell death. The overall result indicates that the methanol extract of leaves of H. hookerianum showed promising baseline information for its potential use as an anti cancer agent.
Sarothralen A and B, new antibiotic compounds from Hypericum japonicum.
Two antibiotic compounds, sarothralen A and sarothralen B, from Hypericum japonicum Thunb. ( Sarothra japonica Thunb.) have been isolated and their structures were elucidated on the basis of spectroscopic data. The compounds contain phloroglucinol and filicinic acid residues.
Sesquiterpene Epoxidation: Rearrangement of (+)-Ledene Epoxy Compounds
Oxidation of (+)-ledene by m-chloroperbenzoic acid was carried out to produce two diastereoisomer epoxides along with another sesquiterpene, an allylic alcohol and an epoxy alcohol, rearrangement products of epoxide in acidic medium. Reduction of epoxides yielded several epoxy ring opening flavoring products, including an ether, an enol, and a diol. The determination of the structure of all the compounds was achieved by 2D-NMR experiments. Keywords: Sesquiterpene; epoxide; sesquiterpenol; oxidation; isomerization; reduction; flavoring compounds; 2D-NMR; MS