Data of PCL-b-P(MMA-DMAEMA)2 characterization and related assays
The data presented here are related to the research paper entitled “PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors” by Franco et al. [1]. Characterization data of PCL-diol, macroinitiator Br-PCL-Br, homopolymers (PMMA and PDMAEMA) and copolymers (batch 1 and batch 2) analyzed by FTIR, SEC and NMR, as well as, characterization of PCL-NS formulation by laser diffraction and DLS analysis, initial nanocapsule formulations and 1C-NC and 2C-NC formulations, including hydrodynamic diameter at different pH media, and DMSO cytotoxicity.
PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors
Abstract A novel poly(e-caprolactone) (PCL) and poly [(methyl methacrylate)- co -(2-dimethylamino)ethyl methacrylate) 2 ] block copolymer, PCL- b -P(MMA- co- DMAEMA) 2 , was synthesized using atom transfer radical polymerization to obtain pH-sensitive nanocapsules intended for drug delivery to solid tumors. The synthesis was performed using PCL:DMAEMA:MMA at two different molar proportions 0.2:10:1 and 0.7:33:1. Nanocapsules prepared using PCL- b -P(MMA- co- DMAEMA) 2 proved sensitivity to pH, presenting z-average hydrodynamic diameters of 73 and 71 nm at pH 7.4, and 96 and 101 nm at pH 5.5, respectively, because of the decrease in the diffusion coefficient of the nanocapsules due to the increase in amine protonation forming ammonium at the surface. Zeta potential at pH 7.2 was + 23 mV for batch 1 and + 13 mV for batch 2. In vitro cytotoxicity of the materials (solutions) and the nanocapsules (dispersed in water) was evaluated in a tumoral cell model (human breast adenocarcinoma cell line, MCF-7) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT). The homopolymers PDMAEMA and PMMA showed low cytotoxicity up to 0.60 and 0.44 mmol L − 1 , respectively. After applying the nanoformulation, the cell viability was not affected for copolymer concentrations up to 340 mg L − 1 . The results showed the new triblock copolymer is a promising material to be used in novel nanocapsule formulations intended for drug delivery to solid tumors.
Biodegradation of poly(ε-caprolactone) in natural water environments
Abstract The environmental degradation of poly(ε-caprolactone)[PCL] in natural fresh water (pond) and in The Baltic Sea is presented in this paper. The characteristic parameters of both environments were measured during experiment and their influence on the biodegradation of the samples was discussed. The loss of weight and changes of surface morphology of polymer samples were tested during the period of incubation. The poly(ε-caprolactone) was more biodegradable in natural sea water than in pond. PCL samples were completely assimilated over the period of six weeks incubation in The Baltic Sea water, but after forty two weeks incubation in natural fresh water the polymer weight loss was about 39%. The results have confirmed that the investigated polymers are susceptible to an enzymatic attack of microorganisms, but their activity depends on environments.