The Clarifying Reagent, or how to make the analysis of milk and dairy products easier
Difficulties with the analysis of opaque milk and dairy products can be resolved by rendering the sample transparent. This allows direct photometric measurements of the reaction mixtures, and all sample pre-treatment steps become unnecessary. For this, several reagents have been reported in literature. Among them, the ‘Clarifying Reagent’ is mainly described here: its composition is given because it is now released from patenting restrictions. Its main property is a low absorbance between 340 and 800 nm. So it can be used in many chemical and enzymatic colorimetric measurements for estimating the quality of milk and dairy products.
Alkaline Phosphatase Activity in Transparent Milk and Cream
Abstract To control pasteurization of milk and cream, a method is described that requires only preparation of two mixtures: a buffer-substrate (diethanolamine/hydrochloric acid containing paranitrophenylphosphate) and a dissolving mixture (triton/cyclohexanone/n-butylamine/hydrochloric acid), the latter is a solution producing transparency at 37°C and also is used to stop the enzymatic reaction. This method increased sensitivity by 2.5 times in comparison with the International Dairy Federation official method. Its high sensitivity and its simplicity favor its use on a large scale by industrial laboratories.
Turbulent boundary layer wall‐pressure fluctuations downstream from an abrupt change in surface roughness
Spectra of wall‐pressure fluctuations beneath a turbulent boundary layer have been investigated immediately downstream from an abrupt change in surface roughness, from slightly rough to very rough. Over the measurable frequency band (400 Hz to 7 kHz), the spectra in the first 150 mm scale on wall variables. This indicates that the motions responsible for the pressure fluctuations measured in this region are very close to the wall. Farther downstream this scaling procedure breaks down. It is suggested that the initially thin internal layer behaves independently of the flow external to it until it has grown appreciably and only then interacts with the outer boundary layer. It was found that, for all frequencies measured, the dimensional spectral level decreases initially then rises to a maximum at 250 mm from the roughness change before decreasing once more. The position of the maximum is close to where the internal layer ceases to have a distinct edge.