Some new reactive polymers for the immobilization of enzymes.
From poly(vinyl alcohol) precursors, various reactive carriers for the immobilization of enzymes were synthesized. As insoluble starting polymers, the following products were used: poly(vinyl alcohol), gels crosslinked with terephthalaldehyde, hydrolyzed beads of crosslinked poly(vinyl acetate), poly(vinyl acetate-co- ethylene) tubes coated with poly(vinyl alcohol), and poly(vinyl alcohol)-containing synthetic pulp. Reactive groups introduced into these carriers or methods for their activation included the diazonium- and isothiocyanato group, and the glutardialdehyde-, BrCN, 2, 4, 6-trichloro-s-triazien, and p-benzoquinone methods. Furthermore, SH-specific reactive groups such as N-substituted maleimide groups or activated mixed disulfides with 2-thiopyridyl groups could be introduced into PVA-polymers. Enzymes like hydrolases (e.g. papain, trypsin, chymotrypsin, urease), oxidoreductases (e.g. glucose oxydase, catalase, glucose-6-phosphate dehydrogenase) as well as the example of transferase hexokinase coimmobilized with glucose-6-phosphate dehydrogenase, were immobilized by reactive poly(vinyl alcohol) carriers. The properties of the immobilized enzymes were investigated.
Reaktive träger zur immobilisierung von enzymen auf der grundlage von polyvinylalkohol
Verschiedene reaktive Trager fur die Immobilisierung von Enzymen wurden auf der Basis von Polyvinylalkohol synthetisiert. Ausgehend von einem Hydrolysat eines Perlpolymeren aus vernetztem Polyvinylacetat sowie von mit Polyvinylalkohol beschicteten Schl¨chen aus Poly(vinylacetat-co-ethylen) bzw. von Polyvinylalkohol enthaltendem Synthesepulp wurden reaktive Trager durch Umsetzung mit 2-(3-Aminophenyl)-1,3-dioxolan und nachfolgender Diazotierung hergestellt. Weiterhin wurden in Gele eines mit Terephthalaldehyd vernetzten Polyvinylalkohols reaktive Isothiocyanatgruppen sowie 2-Thiopyridingruppen enthaltende gemischte polymere Disulfide eingefuhrt. Die Enzyme Papain, Trypsin, Glucose-Oxydase und Katalase wurden immobilisiert und die Eigenschaften der immobilisierten Enzympraparate untersucht. 
 
 
 
On the basis of poly(vinyl alcohol) various reactive carriers for the immobilization of enzymes were synthesized. Starting as well from hydrolyzed beads of crosslinked poly(vinylacetate), as from tubes of poly(vinyl acetate-co-ethylene) which have been coated with poly(vinyl alcohol) resp. from poly(vinyl alcohol) containing synthetic pulp reactive carriers were synthesized by reaction with 2-(3-aminophenyl)-1,3-dioxolane followed by diazotization. Furthermore reactive isothiocyanato groups as well as mixed polymeric disulfides with 2-thiopyridine groups were introduced into gels of poly(vinyl alcohol) which were crosslinked with terephthalaldehyde. The immobilization of enzymes was carried out with papain, trypsin, glucose oxidase, and catalase. The properties of the immobilized enzymes were investigated.
Synthese von verbindungen der cellulose mit eiweißstoffen
Oxy-cellulose wird nach der Azid-methode an Eiweisstoffe gebunden. In ihrer Kettenlange nicht abgebaute Cellulose wird uber ihre Glykolsaure-ather nach der Azid-methode mit Proteinen kondensiert. Es werden zwei Typen von Stoffen erhalten. Bei den kohlenhydratarmen ist jedes Cellulosemolekul von mehreren Proteinmolekulen umgeben. Bei den kohlenhydratreichen treffen mehrere Cellulosemolekule auf jedes Proteinmolekul.