GC-MS ANALYSIS AND ANTICANCER ACTIVITY OF METHANOL EXTRACT OF LEAVES OF HYPERICUM HOOKERIANUM WIGHT & ARN
Objective: The present investigation was an attempt to study the anticancer activity of the methanol extract of the leaves of H. hookerianum. GC-MS analysis revealed that the methanol extract of leaves contained important secondary metabolites viz. oxygenated hydrocarbons and phenolic hydrocarbons. These phytochemicals w e r e p r e s u m e d responsible for various pharmacological actions like antioxidant, and anticancer activities. Cytotoxic changes observed in MCF 7 cell line by the treatment of methanol extract included cell aggregation, cell rounding and cell death. The overall result indicates that the methanol extract of leaves of H. hookerianum showed promising baseline information for its potential use as an anti cancer agent.
Constituents of the leaf essential oil of Hypericum perforatum L. from India
The essential oil of Hypericum perforatum L. of North Indian origin consists of 74% of monoterpene hydrocarbons, 7% of oxygenated monoterpenes, 10% of sesquiterpene hydrocarbons and only 1.5% of oxygenated sesquiterpenes. Ishwarane and α-cuprenene were isolated (besides some well-known monoterpene acetates and other sesquiterpenes) from the high boiling fraction of the oil. The low boiling fraction contained 5- and 6-methylheptan-2,4-dione as trace consistuents, while from the residue two new dimethylchromene derivatives 7-sec-butyl- and 7-isobutyl-2,2-dimethyl-2H,5H-pyrano[4,3-b]pyran-5-one were isolated.
Liquid state 1H high field NMR in food analysis.
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