Histological Studies of Ditylenchus africanus Within Peanut Pods.
Ditylenchus africanus entered the immature pegs and pods of peanut (Arachis hypogaea cv. Sellie) at the peg-connection and subsequently invaded the parenchymatous regions of the hull exocarp and endocarp, and eventually the seed testa. The nematode caused malformations of the cells of infected tissues, cell wall breakage, and cell collapse. The damage appeared to be due to enzymatic activity. In some testae the entire parenchyma region, which aids in protection of the seed, was destroyed. In immature pods, the nematodes moved across the fibrous region of the mesocarp into the hull endocarp. In mature pods, however, the fibrous mesocarp of the hull was lignified and apparently was a barrier to penetration of the inner pod tissues. In late-harvested pods, increased numbers of eggs and anhydrobiotes were found in the hull tissues, and eggs in the seed testa, suggesting the onset of winter survival mechanisms of the nematode.
Survival of Ditylenchus destructor in soil, hulls and seeds of groundnut.
Sununary - The survival of Duylenchus deszruclOr following storage of infected hulls and seeds of groundnut cv. Sellie and infested soi1, and the capacity of D. deszruclOr to undergo complete dehydration and enter in a state of anhydrobiosis was investigated. The results show that D. deszruclOr can survive in the field in the absence of host plants and in hulls left in the field after harvest for at least 28 to 32 weeks. In contras t, nematode numbers in fragmented hulls and fragmented or whole seeds stored in paper bags at 10°C (whole seeds) or 22 oC (fragmented hulls and seeds) decreased with increasing storage time, especially during the first 12 weeks. Very few nematodes survived in whole seeds stored in paper bags at la oC but the surviving nematodes were able to build up large populations and to cause damage. Finally, the results show that the freshness of the tissues played an important role in the efficiency of the soaking method : stored hulls and seeds must be soaked longer than 24 h to make a reliable estimate of the number of nematodes present.
Study on essential drug use status and its influencing factors among cerebral infarction inpatients in county level hospitals of Anhui Province, China
Background and purpose Drug costs is one of the main components of hospitalization expenditure for cerebral infarction inpatients. In China, the National Essential Medicine System (NEMS) was created to relieve the heavy drug-cost burden for patients. The objective of this study was to investigate essential drug-use status and its influencing factors among cerebral infarction inpatients in county-level hospitals of Anhui province, China. Methods Three county-level hospitals were selected through a multi-stage cluster random sampling method. The hospitalization cost data of cerebral infarction inpatients in the three hospitals were extracted from the Anhui provincial information platform of the New Rural Cooperative Insurance System (NCMS), and whether the proportion of essential drug cost in the total drug cost reached the median value of 33.05% which was set as the evaluation index for essential drug-use status. Questionnaires for hospitals and physicians were designed and given to them to assess influencing factors. Results We retrieved the cost data of 2,189 inpatients from the NCMS platform and investigated 51 corresponding physicians in total. The drug costs accounted for 52.6% of the total hospitalization cost, and essential drug costs alone accounted for 37.0% of the total drug costs. The essential drug-cost proportion was high among physicians with a higher recognition degree on NEMS, older age, lower final academic degree, longer work experience and lower professional title. Married physicians and those with tight organizational affiliation also prescribed more essential drugs. Conclusions Increasing the proportion of essential drugs was an effective way to reduce the disease burden for cerebral infarction patients. Perfecting the NEMS, increasing government investment, reinforcing education and propaganda, and formulating relevant incentive and restrictive mechanisms were all effective ways to promote and increase the number of essential drug prescriptions written by physicians.