Chemiluminescent reaction of lucigenin with reducing sugars.
The chemiluminescent reaction, in alkaline solution, of lucigenin with the reducing sugars sorbose, fructose, lactose, glucose, xylose, galactose, arabinose and mannose has been studied. There is a linear relationship (correlation coefficient = 0.996) between the emission intensity and the second-order rate constant for the alkaline oxidation of these sugars. The emission intensity is linearly related to the sugar concentration; at high sugar concentrations (5mM) it is independent of the lucigenin concentration, but at low concentrations (<0.05mM) is linearly related to the lucigenin concentration. These facts support the view that the rate-limiting step is the tautomerization of the sugars to the 1,2-enediol form; the enediol then undergoes reaction with lucigenin.
Chemiluminescence detection for high-performance liquid chromatography of biomedical samples.
During recent years, much progress has been made in the development of high-performance liquid chromatographic (HPLC) detection systems based on chemiluminescence (CL). CL is now one of the most sensitive detection methods in HPLC. For many compounds detection limits in the femtogram to picogram range have been obtained. Several on-line post-column reactions have been used for chemical excitation of the analytes. Some theoretical aspects of CL detection are presented and special attention is devoted to the coupling of CL to flow systems. The influence of the kinetics of the reaction on the sensitivity of the detection system is stressed. The mechanisms and detection systems of the peroxyoxalate, luminol and lucigenin CL reaction are described. A few examples of the use of bioluminescence for HPLC detection are given, and some less common CL reactions used in flow systems are also mentioned. Many biomedical and related applications are shown. Possibilities and limitations of the various reactions and detection systems are evaluated.
Rotational Analysis of the CrO Bands
The (0, 1), (0, 0) and (1, 0) bands of the CrO, which appear in the red and yellow region, are analysed rotationally, and we have been able to show that these bands owe to the transition 5 Π- 5 Π. We have shown the theoretical formulae for the quintet state, which can be applied in the case of nearly (a), and obtained the molecular constants as follows,