Production of cucumber fruits from the culture of ‘Marketmore-76’ plantlets
A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar ‘Marketmore-76’ in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leaves and flowers after another 4 to 8 weeks in culture. Administration of compressed air at a 300 ml/min flow rate for 30 min 10 or 15 times a day induced the development of parthenocarpic fruits from flowers. Fruits, up to 170 mm long by 35 mm diameter, were obtained within 30 to 45 d after flower opening.
Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens
In vitro regeneration protocol for Anethum graveolens (Apiaceae) was developed using leaf explants. MS basal medium used in experiments was augmented with various hormones for caulogenic and rhizogenic response. The optimum callus induction (100%) was obtained by leaf explants on MS media fortified with BA (0.5 mg l−1) singly and in combination with NAA (0.1 and 0.2 mg l−1). BA at 0.5 mg l−1, KN at 1.0 mg l−1 and NAA at 0.1 mg l−1 induced highest number of multiple shoots (10.0 ± 0.25) per explant and they also showed in vitro flowering within 3 weeks of culture. Influence of adenine sulfate on regeneration frequency of callus was evaluated. The highest frequency of rooting (100%) with 6.0 ± 0.25 roots per explants was obtained in one-fourth strength MS medium supplemented with 1/4 MS + IBA 0.5 mg l−1 within 4 weeks of transfer to the rooting medium. In vitro flowering (35%) was obtained with MS fortified with BA alone and also in combination with KN and NAA (5.3 ± 0.42 flowers per explants). In vitro flowering response was tested with different carbohydrates (fructose, glucose, mannose and sorbitol) and optimized. Hardening was successfully attained under controlled conditions inside the plant tissue culture room. The proposed method could effectively be applied for the conservation and clonal propagation to meet the pharmaceutical demands of this medicinally important species.
A Study on Greenhouse Automatic Control System Based on Wireless Sensor Network
The system proposed in this paper collects temperature of leaves and humidity on leaves of crop. As well as greenhouse environmental information such as temperature, humidity, etc. Crop diseases, especially, have deep relationship not only with indoor environmental factors but also with humidity lasting time on leaves and temperature of leaves. Accordingly, monitoring crop itself is as important as monitoring indoor environments. Using these collected greenhouse environmental data, indoor environments can be more effectively controlled, and monitoring crop itself can contribute to improve productivity and to prevent crops from damages by blight and harmful insects. In addition, it will be possible for farmers to do control plant growth through closely studying relationship between indoor environmental information and monitored information on crop itself. Collected data can be stored to database either in server installed in greenhouse or to remote server. It is made possible to collect information and control effectively and automatically greenhouse in the site or from a remote place through web browser. System components are: temperature sensor, humidity sensor, leaf temperature sensor, leaf humidity sensor, Zigbee based wireless sensor node, relay nodes for automatic control, and data server to store greenhouse information. The system is implemented using low power wireless components, and easy to install.