2,3,7-Trimethcxy-8,9-methylenedioxypavinan — A new alkaloid from Thalictrum strictum. II
F rom the epigeal pa r t of Th. s t r i c tum we have i so la ted a new unidentified base B (I). The alkaloid (I) is readi ly soluble in methanol , ethanol, and acetone and when the solvents are evapora ted it s epa ra t e s out in the fo rm of a faint yellowish oil . After chromatography twice on a column of a lumina and concentrat ion of the e therea l eluate, compound (I) c rys ta l l i zed in the fo rm of t r anspa ren t p r i s m s with mp 144-145°C, [o~]~ 1 7 4 ° (c 0.977; methanol).
THE ALKALOIDS OF PAPAVERACEOUS PLANTS: LIV. THE STRUCTURE OF ESCHSCHOLTZINE
Eschscholtzine, a new alkaloid from Eschscholtzia californica, has been shown to be the bismethylenedioxy analogue of N-methylpavine (argemonine). The mass spectrometric fragmentation of alkaloids of the pavine type is discussed.
HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment.
A novel fast isocratic reversed-phase HPLC method for simultaneous determination of chlorhexidine and its degradation product p-chloroaniline was developed. Zorbax SB Phenyl column (75 mm x 4.6 mm, 3.5 microm) was used for the separation. Mobile phase composed of acetonitrile and buffer solution of 0.08 M sodium phosphate monobasic containing 5 ml of triethylamine (0.5%) and adjust with 85% phosphoric acid to pH 3.0 in ratio 35:65 (v/v) pumped isocratically at flow rate 0.6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory.