Cross-reactivity and antigenic structure of the C 5 component of human complement
W e tr ied to f ind some corre la t ion be tween t he syn thes i s of p roazu lenes in t he essent ia l oil of s o m e species oi the genus Achilles and t he n u m b e r of c h r o m o s o m e s in thei r somat ic cells: 12 species were i nves t i ga t ed ; in each case, samples of wild and cu l t iva t ed p l an t s of local and foreign origin were inc luded (table). P roazu lenes were ident i f ied in a wa te r ex t r ac t ob ta ined f rom t he herbage, us ing t he m e t h o d of S tah l based on color reac t ion of azulenes wi th p d i m e t h y l a m i n o benza ldehyde [6]. The n u m b e r of ch romosomes in mi to t ic ceils in vege t a t i on or roots af ter ge rmi na t i on was de t e rmined b y t he m e t h o d ol E h r e n d o r f e r [7]. The e x p e r i m e n t s showed t h a t the genus Achilles is polyploid wi th the base n u m b e r x = 9 of ch romosomes . P roazu lenes were p r e sen t in all s amples of the species Achilles coZlina Becke t and Achilles pannonica Scheele; t he former is t e t r a ploid (2ar 36), t he la t t e r hexa or octoploid ( 2 n = 54 or 2n = 72; table). O the r species (except Achilles miile]oIium L.
Immunochemical Techniques for the Identification and Estimation of Macromolecules
(Chapter headings) Preface. Chapters: 1. Introduction 2. Immunodiffusion in gels 3. Antisera 4. Techniques of immunodiffusion 5. Techniques of immunoelectrophoresis 6. Visualization and interpretation of precipitates in gels 7. Immunoadsorbent techniques 8. Radio-immunochemical techniques 9. Enzymo- and metallo-immunoassays 10. Difficulties and artifacts arising in gel diffusion and in immunoelectrophosesis 11. Immunochemical reactions in free buffer systems 12. Immunofluorescence techniques 13. Complement fixation assays 14. Application of lymphocytes for tracing antigens 15. Miscellaneous topics in immunochemistry 16. Appendices References. Subject index.
Highly enantio- and diastereoselective synthesis of β-methyl-γ-monofluoromethyl-substituted alcohols.
Enanatiopure β-methyl-γ-monofluoromethyl alcohols were prepared from the allylic alkylation between fluorobis(phenylsulfonyl)methane with Morita-Baylis-Hillman carbonates. The reaction was catalyzed by using the Cinchona alkaloid derivative, (DHQD)(2)AQN. The origin of the stereoselectivity was verified by DFT methods. Calculated geometries and relative energies of various transition states strongly support the observed stereoselectivity.