The Composition and Antimicrobial Activities of Cyperus conglomeratus, Desmos chinensis var. lawii and Cyathocalyx zeylanicus Essential Oils
The essential oil compositions of the rhizomes of Cyperus conglomeratus (Cyperaceae) collected from Oman and the leaves of two Annonaceae plants, Desmos chinensis var. lawii and Cyathocalyx zeylanicus collected from India were studied by GC, GC-MS and 13C NMR spectroscopy. Twenty-six compounds, representing 84.4% of the oil were identified in C. conglomeratus, where eugenol (31.3%), α-cyperone (10.5%) and cyperotundone (8.4%) were the major compounds. Twelve compounds, constituting 100%, were identified in D. lawii oil, of which benzyl benzoate (58.7%), β-caryophyllene (23.2%), limonene (4.9%) and α-humulene (4.0%) were the major constituents. Thirty-two compounds, comprising 98.0%, were identified in C. zeylanicus oil, of which β-caryophyllene (21.6%), α-pinene (20.4%) and E-β-ocimene (11.8%) were the major components. The antibacterial and antifungal activities of the oils were tested against a panel of five bacterial and two fungal strains. The oils showed moderate activity against all the tested microbial strains. The minimum inhibitory concentrations of the oils were also determined.
UPLC-qTOF-MS Phytochemical Profile and Antiulcer Potential of Cyperus conglomeratus Rottb. Alcoholic Extract
Cyperus has been commonly used as a multi-use medicinal plant in folk medicine worldwide. The objectives of our study were to determine the different metabolites in the Cyperus conglomeratus Rottb. methanol extract, and to assess its in vivo gastroprotective effect in ethanol-induced gastric ulcer model in rats. Serum levels of galactin-3 and TNF-α were employed as biochemical markers. To pinpoint for active agents, comprehensive metabolites profiling of extract via UPLC-qTOF-MS/MS was employed. A total of 77 chromatographic peaks were detected, of which 70 were annotated. The detected metabolites were categorized into phenolic acids and their derivatives, flavonoids, stilbenes, aurones, quinones, terpenes, and steroids. Rats were divided into six groups; healthy control, ulcer control, standard drug group, and 25, 50, 100 mg/kg of C. conglomeratus treated rats. Pre-treatment with C. conglomeratus alcohol extract significantly reduced galactin-3, and TNF-α in ethanol-induced ulcer model at 25, 50, and 100 mg/kg. Further histopathological and histochemical studies revealed moderate erosion of superficial epithelium, few infiltrated inflammatory cells, and depletion of gastric tissue glycoprotein in the ulcer group. Treatment with the extract protected the gastric epithelial cells in a dose-dependent manner. It could be concluded that C. conglomeratus extract provides significant gastroprotective activity in ethanol-induced gastric ulcer and ought to be included in nutraceuticals in the future for ulcer treatment.
Flavonoid aglycones in the resin of Hazardia squarrosa var. Grindelioides
Abstract Thirty three flavonoid aglycones have been identified from the external stem and leaf resin of Hazardia squarrosa var. grindelioides by TLC co-chromatography with authentic samples. The occurrence of compounds with oxygenation at C-6 and C-5′, which are also found in the genus Haplopappus, supports the previously believed close relationship between these two genera.