Chromatographic separation of putative precursors of cholestanol
This paper describes convenient syntheses for labeled and unlabeled cholest-5-en-3-one, cholest-4-en-3-one, epicholesterol, cholest-4-en-3 beta-ol, and cholest-4-en-3 alpha-ol. The thin-layer chromatography, high-performance liquid chromatography, and gas-liquid chromatography of these compounds and of cholestanol and epicholestanol are also described. The synthesized compounds are potential precursors in the biosynthesis of cholestanol in mammals.
Thin-layer chromatographic procedures for lipid separation.
This review focuses on the thin-layer chromatographic (TLC) separation aspects of lipid analysis. Since the space limitations do not permit, the quantitative aspects of the analyses are not discussed at length although some indications of appropriate methodology and detection reagents will be indicated. Many separations carried out by TLC have the prerequisite of proper sample preparation. Using proper sample clean-up prior to TLC enables one to carry out precise separation as well as sensitive quantitation. Thus, the discussions are divided into the two main topics--sample preparation and TLC. Examples of applications are limited to those which illustrate the capabilities of the technique as well as practicability. Since there are a number of reviews in the literature, the discussions herein are focused mainly on reports after 1985.
Physical Chemistry for the Life Sciences
PART 1 - BIOCHEMICAL THERMODYNAMICS PART 2- THE KINETICS OF LIFE PROCESSES PART 3 - BIOMOLECULAR STRUCTURE PART 4 - BIOMOLECULAR SPECTROSCOPY