Evaluation of the botanical origin of estonian uni- and polyfloral honeys by amino acid content.
The free amino acid content of 61 honey samples from Estonia has been determined by HPLC-UV with precolumn derivatization with diethyl ethoxymethylenemalonate. Analyzed samples were seven types of unifloral honeys and polyfloral honeys. The main amino acids found in Estonian honeys were proline and phenylalanine. The resulting data have been analyzed by t test and principal component analysis (PCA). t Test revealed that some amino acids (alpha-alanine, beta-alanine, asparagine, gamma-aminobutyric acid, glutamine, glycine, histidine, ornithine, phenylalanine, proline, serine, and tryptophan) are more potent for assigning honey botanical origin than others. PCA enabled differentiation of some honey types by their botanical origin. In the space of the two first principal components, heather honeys form a cluster that is clearly separable from, for example, polyfloral honeys. It is concluded that analysis of the free amino acid profile may serve as a useful tool to assess the botanical origin of Estonian honeys.
Relationship between interannual variation of amino acid profile and pollen content in honey from a small Argentinian region.
With the objective of evaluating the utility of the amino acid profile in the characterization of honey samples, 39 honey samples of two different harvests from a particular production zone in Córdoba, Argentina, were analyzed. Multivariate statistical techniques, such as principal component analysis (PCA), cluster analysis (CA), and multiple correspondence analysis (MCA), were applied to verify the correlation among the amino acid profiles, pollen percentages, and different harvests. PCA, CA, and MCA demonstrate the presence of differences of amino acid profiles between samples of the two harvests, such differences being mainly due to differences in pollen availability. Variation of the flora surrounding the apiary due to agricultural practices makes the analysis of amino acid profile typical for those cases with stabilized flora.
Effect of Soybean Cyst Nematode (Heterodera glycines) on Yield of Resistant and Susceptible Soybean Cultivars Grown in Ohio.
Soybean (Glycine max) producers in Ohio rarely use soybean cyst nematode (Heterodera glycines, SCN)-resistant cultivars because of concerns over limited yield potential and lack of resistance to Phytophthora sojae. A two-year study was initiated to determine grain yield and nematode population increase on soybean cyst nematode-resistant cultivars in maturity groups II and III in production fields. Sites differed in soil texture, nematode densities, and P. sojae infestation at a number of locations in Ohio. Soil was assayed for nematode densities before planting and at harvest. Yields of resistant cultivars averaged 0% to 18% higher than those of susceptible cultivars in fine-textured soils with average preplant populations ranging from 463 to 14,330 SCN eggs/100 cm(3) soil. In coarse-textured soils, yields of susceptible cultivars were 21% to 56% less than the resistant cultivars with average preplant densities ranging from 1,661 to 15,558 SCN eggs/100 cm(3) soil. The reproductive index ranged from 0.1 to 5.5 for resistant cultivars and 0.4 to 112 for susceptible cultivars. In 1993, yield of P. sojae-susceptible, nematode-resistant 'Asgrow A 3431' was as high as yield of the P. sojae-resistant, nematode-susceptible cultivar 'Resnik' in a Phytophthora-infested field. The nematode-resistant cultivars Madison Experimental 131527 and Asgrow A3431 had higher yields than AgVenture AV1341 and susceptible cultivars Resnik and Kenwood when compared over five nematode-infested sites. Nematode-resistant cultivars were found to be excellent alternatives to currently grown susceptible cultivars for managing SCN where group III cultivars are used. However, better cultivar alternatives may be needed for sites with combined Phytophthora root rot and cyst nematode problems.