Measurement technology of operation region of EVA spacesuit
Operation ability of astronaut in spacesuit is a key parameter in Extra-vehicular activities. The operation region of people in the spacesuit was tested by 3-jointed mechanical measuring arm, which combined force-torque sensors in the handle, and magnetic brakes installed in every joint. Mathematical model of the measuring arm was established to transform the signals of sensors to the data required. The errors were analyzed and the system was calibrated and compensated. All tests were aided by computer, and data obtained were processed by software developed based on VC++, OpenGL and Matlab. An exact 3- D operation region of left hand, right hand and both hands was obtained by a method of reverse engineering. The measurement supplied solid support in space engineering design.
The Soviet/Russian spacesuit history
Abstract The European spacesuit system (ESSS) initiated by the European Space Agency (ESA) in the late 1980s had many similarities with the Soviet/Russian ORLAN spacesuit system, due to the Hermes system requirements. First, direct contacts in 1989 permitted closer comparison of the two suit systems, and soon the ORLAN manufacturer Zvezda could be contracted as support to the European spacesuit team. In particular, the suit enclosure design and predevelopment testing and operational analysis were performed in close cooperation between Zvezda and the European team under Dornier. With the changing system requirements and a closer cooperation between ESA and the new Russian Space Agency (RKA) a new joint spaceplane/stations mission scenario came about. This scenario could be served by one spacesuit system, EVA SUIT 2000, which was to be jointly developed by a team headed by Zvezda and Dornier for ESA and RKA. ORLAN-DMA and ESSS experience and hardware were the initial platforms for these activities to create a new generation spacesuits for the Mir 2 and later the ISSs. A suit demonstrator was manufactured and tested by the end of 1994 when ESA stopped its spacesuit development activities and the joint EVA SUIT 2000 project was terminated. However, many of the features designed, manufactured and tested for the EVA SUIT 2000 were then implemented by Zvezda in the new Russian spacesuit system ORLAN-M, now in full operation onboard the ISS.
Cross talk between redox signalling and metabolic activity of osteoblasts and fibroblasts in the presence of hydroxyapatite-based biomaterials influences bone regeneration
Regeneration of bone tissue defects that result from metabolic disorders, including periodontal diseases, can be supported by biomaterials based on hydroxyapatite. Despite of good biocompatibility of biomaterials they can cause oxidative stress and inflammatory processes as a result of mechanical interaction with surrounding tissues. Because osteoblasts are responsible for bone regeneration process in which gingival fibroblasts may also participate, the aim of the work was to investigate the influence of hydroxyapatite-based biomaterials (allogeneic and xenogeneic) and biomaterials combined with enamel matrix derivative (Emdogain) on osteoblast and fibroblast redox balance in the context of osteoblast proliferation and differentiation.
The results showed that examined substitutes were not cytotoxic in vitro, but affected redox balance of osteoblasts and fibroblasts (ROS level increase and GSH level decrease) which led to oxidative stress (MDA and protein carbonyl groups level increase) resulting in an increase of the Nrf2 and NFκB expression. The consequence of these changes was partial inhibition of proliferation and osteoblast differentiation. Emdogain alone and combined with biomaterials decreased ROS generation and increased GSH level in both osteoblasts and fibroblasts leading to reduction of transcription factors expression especially proinflammatory NFκB, which promoted osteoblast differentiation and mineralization process.