Preparation of 3-deoxy-3-deuteroestrone and 3-deoxy-4-14C-estrone.
3-Deoxy-3-deuteroestrone (1,3,5(10)-estratrien-17-one-3-d) and 3-deoxy-4-14C-estrone (1 ,3 ,5(10)-estratrien-17-one-14C) have been prepared. The mechanism of reductive dehalogenation of aryl iodides with lithium aluminum deuteride is discussed. The 13C-NMR spectrum of 3-deoxy-3-deuteroestrone is discussed.
4-Hydroxyestrone: A new metabolite of estradiol-17β from humans
Abstract 4-Hydroxyestrone has been identified, by reverse isotope dilution, as a urinary metabolite after injection of a mixture of 4- 3 H- and 4- 14 C-estradiol-17β into a 23 year old woman and a 39 year old man. This new metabolite accounted for 1.1% of the - 14 C dose administered to the woman and 0.51% of the - 14 C dose administered to the man. 7.3% Of the 3 H dose was liberated into the body water pool of the woman and 6.1% into that of the man. The yields of radioactive estrone, estradiol-17β, estriol, 2-hydroxyestrone, 2-methoxyestrone and 2-hydroxyestrone 3-methyl ether were also measured in both the Ketodase and Glusulase hydrolyzed urinary fractions from both subjects.
THE IRON-ACETOCARMINE METHOD OF FIXING AND STAINING CHROMOSOMES
A short description of this procedure was given in Vol. 55 of the American Naturalist, pp. 573â€”574. The method has now been used almost daily for five years, and certain improvements have suggested themselves. The solution can be prepared by mixing about equal volumes of glacial acetic acid and water. To this is added powdered carmine in excess. The liquid is then â€ ? just brought to boil, cooled, and decanted. To the red solution there are then added a few drops of a solution of ferric hydrate in 50 per cent. acetic acid, to act as a mordant. The amount of iron varies with different objects. Too much produces a precipitate in a short time. The more