The Tick Ixodes kaiseri (Acari, Ixodidae) in the North Caucasus and Transcaucasia According to the Material from the Collection of Stavropol Anti-Plague Institute
To date, the North Caucasus, particularly the territory of Stavropol Territory, is traditionally indicated as the habitat of the tick Ixodes crenulatus Koch, 1844. I. kaiseri Arthur, 1957 was previously known only from a single site in the Eastern Ciscaucasia. Re-examination of collections from the North Caucasus and Transcaucasia has shown that the overwhelming majority of the specimens from these regions belong to the species I. kaiseri, allowing the revision of common views on the range boundaries of both species.
Molecular epidemiological study on ticks and tick-borne protozoan parasites (Apicomplexa: Cytauxzoon and Hepatozoon spp.) from wild cats (Felis silvestris), Mustelidae and red squirrels (Sciurus vulgaris) in central Europe, Hungary
Background Among live wild mammals adapted to urban and peri-urban habitats in Europe, members of the families Felidae, Mustelidae and Sciuridae deserve special attention as pathogen reservoirs because all of these families include members that are kept as pets. We report here the results of our study on two important groups of tick-borne protozoan parasites in ticks and tissues of wild cats, mustelids and red squirrels. Methods DNA was extracted from the tissues of carnivores (wild cats, mustelids; n = 16) and red squirrels ( n  = 4), as well as from ixodid ticks ( n  = 89) collected from these hosts. These DNA extracts were screened for piroplasms and Hepatozoon spp. using conventional PCR analysis and sequencing. In addition, 53 pooled samples of 259 questing Haemaphysalis concinna ticks were evaluated for the presence of Hepatozoon DNA, followed by phylogenetic analyses. Results One wild cat was found to be coinfected with Cytauxzoon europaeus and a new genotype of Hepatozoon felis , and two additional wild cats were infected with H. felis from a different phylogenetic group. In mustelids, Hepatozoon martis and two further Hepatozoon genotypes were detected. The latter clustered separately, close to others reported from eastern Asia. In addition, Hepatozoon sciuri was detected in red squirrels. Morphologic and molecular analyses verified eight tick species. One wild cat was infected with a H. felis genotype that was significantly different from that in Ixodes ricinus females infesting this cat. Only three pools of questing H. concinna nymphs tested positive for Hepatozoon , one of which contained H. martis . Conclusions This study provides the first evidence of the occurrence of any Cytauxzoon species and of three Hepatozoon species in Hungary. In addition to H. martis , two further mustelid-associated Hepatozoon genotypes were detected, one of which was new in terms of phylogenetic and broader geographical contexts. This may be the first indication that H. felis genotypes from both of its phylogenetic groups occur in Europe. This also appears to be the first evidence of H. felis and C. europaeus coinfection in felids in Europe, and of autochthonous H. felis infection in wild cats north of the Mediterranean Basin. New tick–host associations were also observed in this study. Based on the results, H. felis and H. martis might survive transstadially in I. ricinus and H. concinna , respectively. Graphical Abstract
The role of early lithification in development of chalky porosity in calcitic micrites: Upper Cretaceous chalks, Egypt
Abstract Petrological and geochemical examination of Upper Cretaceous chalks from the Abu Roash area and Bahariya Oasis (Egypt) provide insights into factors which control porosity development in fine-grained calcitic carbonates. Petrological studies indicate that primary skeletal fabrics of coccoliths and foraminifera in micrites of Abu Roash are well preserved. In contrast, primary skeletal fabrics of the Bahariya Oasis chalks are pervasively altered. Geochemical analysis of these chalks reveals a striking contrast in composition. The high porosity chalks at Bahariya Oasis have depleted isotopic values ( δ 13 C = −5.0‰, δ 18 O = −8.9‰ PDB ) and low concentrations of trace elements (Sr and Na). In contrast, the low porosity chalks at Abu Roash are less depleted in terms of their isotopic values ( δ 13 C = 1.0‰, δ 18 O = −4.0‰ ) and possess elevated concentrations of Sr, Na, Fe and Zn. Importantly, fracture and collapse veins, present only at Abu Roash, are filled with calcite whose δ18O value is coincident with that of altered micrites at Bahariya Oasis. The lithic fragments cemented by this calcite, however, retain compositions identical to other lithified limestones at Abu Roash. Differences in fabric and chemistry of the pelagic marine micrites observed between Abu Roash and Bahariya Oasis reflect the degree of early lithification and compaction prior to exposure and meteoric diagenetic alteration associated with regional Late Cretaceous/pre-Eocene subaerial unconformity.