Influence of pozzolanic activity of coal gangue on hydration products in coal gangue-cement system
Qualitative and quantitative analysis of the composition in the paste of complex cements have been determined by means of X-ray diffraction (XRD),differential scanning calorimetry (DSC) and directly-heating method. Results show that the composition of hydration products in coal gangue-cement complex system is similar to that in pure cement system, but the content of hydration products for per unit cement in former is higher than that in later at the early hydration stage. The pozzolanic reaction between coal gangue and cement hydration products mainly occurs in 3d. The content of Ca(OH)2 and that of C-S-H in the paste are related to the activity and the content of coal gangue. The content of C-S-H in high activity coal gangue-cement system is related to the activity of coal gangue rather than the content, but that is opposite in low activity one.
EFFECT OF MINERAL ADMIXTURE ON DURABILITY OF CONCRETE
In order to improve the durability of concrete against corrosion,adding the fly ash and slag powder in the concrete.Experimented on influence of fly ash and slag powder on freeze resistance,impermeability of concrete,sulfate resistance,chloride resistance,the result shows that,adding fly ash and slag powder in concrete can make the concrete have the properties of easy mixing and fluidity,and can endow a series high performances of durability,such as high freeze resistance,high impermeability,high sulfate resistance and high chloride resistance.The effect of slag powder on durability of concrete is better than fly ash.
Cross talk between redox signalling and metabolic activity of osteoblasts and fibroblasts in the presence of hydroxyapatite-based biomaterials influences bone regeneration
Regeneration of bone tissue defects that result from metabolic disorders, including periodontal diseases, can be supported by biomaterials based on hydroxyapatite. Despite of good biocompatibility of biomaterials they can cause oxidative stress and inflammatory processes as a result of mechanical interaction with surrounding tissues. Because osteoblasts are responsible for bone regeneration process in which gingival fibroblasts may also participate, the aim of the work was to investigate the influence of hydroxyapatite-based biomaterials (allogeneic and xenogeneic) and biomaterials combined with enamel matrix derivative (Emdogain) on osteoblast and fibroblast redox balance in the context of osteoblast proliferation and differentiation.
The results showed that examined substitutes were not cytotoxic in vitro, but affected redox balance of osteoblasts and fibroblasts (ROS level increase and GSH level decrease) which led to oxidative stress (MDA and protein carbonyl groups level increase) resulting in an increase of the Nrf2 and NFκB expression. The consequence of these changes was partial inhibition of proliferation and osteoblast differentiation. Emdogain alone and combined with biomaterials decreased ROS generation and increased GSH level in both osteoblasts and fibroblasts leading to reduction of transcription factors expression especially proinflammatory NFκB, which promoted osteoblast differentiation and mineralization process.