Effect of reduced water activity on vegetative growth of cells and on germination of endospores of Sporolactobacillus.
Growth of vegetative cells and germination of endospores of Sporolactobacillus at reduced water activity (aw) values were studied with NaCl and glycerol as humectants, and compared with Bacillus cereus and Clostridium sporogenes. With NaCl the highest aw for complete inhibition of both growth of vegetative cells and germinated spores of Sporolactobacillus was 0.955. With glycerol, growth of vegetative cells was completely inhibited at 0.905 as compared to 0.880 for vegetative growth resulting from germinated endospores. The mechanism of this phenomenon cannot be explained yet. NaCl appeared to have a toxic effect, in addition to inhibition by reduced aw per se.
The Effect of Water Activity and the aw Controlling Solute on Spore Germination of Bacillus stearothermophilus
The germination of spores of Bacillus stearothermophilus was studied in nutrient broth in relation to the water activity (aw) of the medium, the nature of the aw controlling solutes glycerol, sucrose, KCl, and NaCl, and temperature. Quantitation of germination was based on the change of the phase-bright spore to phase-dark. Activation of spores was by exposure to 100°C/10 min in a medium of the same composition as that used for germination. 
 
Of the four solutes used, sucrose proved most inhibitory to germination, especially in the upper part of the temperature range 38-75°C, glycerol was the most favourable whereas KCl and NaCl, whose effect was almost identical, occupied an intermediate place. The glycerol effect became more pronounced as the aw of the medium decreased towards 0.960, becoming inhibitory thereafter. 
 
The solute effect on spore germination followed a pattern that related to the class of solute, i.e. electrolyte or non-electrolyte, and its cell penetration characteristics. 
 
Solute penetration during heat activation and germination was considered as the major germination factor and was associated with the osmoregulation mechanism within the spore proposed recently as the basis of spore dormancy and resistance.
Study of External Parasites Infesting Carcharhinus Melanopterus Fish From The Red Sea In Jeddah, Saudi Arabia
Parasitic diseases are harmful and limiting factors in breeding and rearing ornamental fish industry, such as the Monogenea ( Dactylogyrus sp and Erpocotyletiburonis ) and copepod ( Pandarus sinuatus  and Anchistrotostangi ). The copepod and the monogenean are gill parasites found on the shark; CarcharhinusMelanopterus . The primary purpose of this study was to determine the parasitic infections of sharks in red sea in Jeddah. For this purpose, 19 female C. Melanopterus and 14 male C. Melanopterus were first examined for ectoparasites using wet mount under a light microscope. All male sharks had Monogenea and/or copepod in their gill and only 3 females haven’t infections. Then, we aimed to find a combination of copepod and monogenea infection in C. Melanopterus gills. 50% of male and 42% of female were infected by copepod and monogenea. Our findings revealed that the protozoan infections are very common among sharks. Although, no gross pathology was observed among infected shark, but it is likely that in case of any changes in the environment, then parasitic infections could be harmful.