Qualitative analysis of Cu2+, Co2+, and Ni2+ cations using thin-layer chromatography.
The M(DEDTC)2 (M = Cu, Co, or Ni) and M(PyDTC)2 (M = Cu or Co) complexes prepared by reactions of sodium diethyldithiocarbamate and ammonium pyrollidinedithiocarbamate with metal (II) nitrates are examined for qualitative analysis and separation using thin-layer chromatography systems. These complexes and their mixtures are spotted to the activated thin layers of silica gel 60GF254 (Si-60GF254) with a 250-microm thickness. Pure toluene and a toluene-cyclohexane mixture (3:1, v/v) are used as mobile phases for running of the complexes. These chromatographic systems are successfully used for qualitative analysis of corresponding metal cations and separation of components in both M(DEDTC)2 and M(PyDTC)2 complex mixtures.
Cation analysis with thin layer chromatography
Reported here is an adaptation of thin layer chromatography to an undergraduate laboratory operation for cation analysis.
Synthesis of Dihydrostreptobiosamine
The isopropylidene derivative (4) of benzyl dihydrostreptoside and 3,4,6-tri-O-acetyl-2-amino-N-anisylidene-2-deoxy-α-L-glucopyranosyl bromide (7) were prepared, and they were coupled by a modified Koenigs-Knorr condensation. After hydrolysis of the N-ankylidene group of the product, N-methoxycarbonylation followed by N-methylation was accomplished to afford the fully protected derivative (9) of dihydrostreptobiosamine, which was, after deblocking, led to benzyl dihydrostreptobiosaminide and dihydrostreptobiosamine.