Analytical variation in metabolic profile testing
Summary Results are presented demonstrating quality control of the analytical procedures used during the application of metabolic profile tests to dairy herds over a period of 3 years. The significance of analytical variance is discussed in relation to the average population variance in dairy herds. It is concluded that analytical variance can be restricted to reasonable proportions providing that careful quality control is continuously employed.
A comparison of anticoagulants for the analysis of glucose concentration and for haematological measurements in bovine blood.
Abstract Blood samples were taken from 18 cows into a potassium oxalate-sodium fluoride mixture, or a potassium oxalate-ammonium oxalate-ammonium fluoride mixture, or into ethylene diamine tetraacetate. Haematocrit, and the concentrations of glucose in the blood and blood plasma, were determined in each sample. Blood samples from another 6 cows were taken into the same anticoagulant mixtures and stored at 4 °C., room temperature, or 37 °C. The concentrations of glucose in the blood and plasma, haematocrit, mean corpuscular volume, erythrocyte count, and haemoglobin concentrations were determined at intervals of up to 96 h. after collection. Blood taken into the potassium oxalate-sodium fluoride mixture had reduced values for haematocrit, but there were no significant differences in haematocrit between samples taken into the other 2 anticoagulants. Little difference in the glucose concentrations of whole blood was apparent in samples taken into the 2 fluoride containing anticoagulant mixtures, but plasma concentrations of glucose were significantly lower in the samples taken into the potassium oxalate-sodium fluoride mixture. Consistent values for all determinations were obtained up to 96 h. when samples were stored at 4 °C. Some haemolysis and glycolysis occurred during storage at higher temperatures. This was more severe in the potassium oxalate-sodium fluoride mixture than in the potassium oxalate-ammonium oxalate-ammonium fluoride mixture. It is concluded that the latter is preferable for blood to be used for the determination of glucose concentrations and for haematological measurements in the metabolic profile testing of dairy herds.
Production of new antituberculosis drug and other medical preparations by electron beam treatment
Abstract Possibility of electron beam usage for immobilization of hydrazidum of an isonicotinic acid (HIA) on dextrane was studied to produce a drug for treatment of a tuberculosis. As a result of irradiation of dextrane the processes of oxidation and formation of polysaccharide occur due to carbonyl groups which are to link isoniasidum. The HIA immobilized on dextrane may be used as an effective medical preparation having prolonged action for action on endo and extracellular population of Micobacterium tuberculosis .