Assessment of Bacteriological Quality of Sold Meat in the Butcher Shops of Adigrat, Tigray, Ethiopia
2 Abstract: The study was carried out from November 2011 to April 2012, through which purposive sampling methodology was used to assess the bacteriological quality of sold meat in abattoir and butcher shop of Adigrat. On a total of 80 meat samples (20 samples from each stage; abattoir and butcher house of hanging meat, minced meat and refrigerated meat). The mean values of bacterial load of hanging meat, minced meat, abattoir meatand refrigerated meat were 5.5x10 , 6.5x10 , 3.2x10 and 3x10 CFU/g respectively indicating high 777 7 mean values of microbial load in the hanging meat and minced meat sales with statistically significant difference (P<0.05).Theresult also indicated that 13.75, 46.25 and 40% of the meat are acceptable,marginally acceptable and unacceptable respectively. The major bacterial pathogens isolated were Escherichia coli; Staphylococcus aurous Streptococcus species and non-lactose fermenting bacteria. Most of the isolated bacteria were susceptible for the commonly used antimicrobials.Careless handling of meat at the slaughtering places and butcher shops affect the quality of meat which indicated as the presence of contamination. Therefore, particular attention to meat hygienic should be strengthened in both butcher houses and abattoirs workers.
Assessment of Microbiological Safety and Quality of Minced Meat and Meat Contact Surfaces in Selected Butcher Shops of Addis Ababa, Ethiopia
Food-borne pathogens are one of the leading causes of illness and death particularly in developing countries. This study was aimed at analyzing the hygiene indicator microorganisms and pathogens of minced meat and contact surface materials in butcher shops in Addis Ababa, Ethiopia. Additionally, a checklist was applied to evaluate the hygiene condition of the establishments, and a questionnaire/checklist was used to assess food safety knowledge of the food handlers. This study has indicated that the mean microbial counts (total aerobic mesophilic, staphylococci, Enterobacteriaceae, total coliforms, fecal coliforms, aerobic spores and yeasts/molds) of the minced meat and contact surface materials in butcher shops ranged between 2.35 and 6.50 log·cfu/g and between 1.80 and 6.30 log·cfu/cm2, respectively. The mean microbial counts of minced meat samples taken in the morning and afternoon showed statistically significant differences (p<0.05). The prevalence of E. coli, Salmonella, and S. aureus in minced meat and contact surface samples was exhibited as 43.75 and 29.17%, 6.25 and 4.17%, and 37.50 and 37.50% in that order. The study has indicated that minced meat samples and contact surface materials had higher microbial load with poor personal and work area sanitation. Low knowledge of food handlers in the butcher shops and broken cold chain have also been found as major contributing factors for the contamination of beef.
Avian infectious bronchitis.
Avian infectious bronchitis (IB) is caused by the gammacoronavirus infectious bronchitis virus (IBV). The virus causes infections mainly in chickens and is a significant pathogen of commercial meat and egg type birds. IB is an acute, contagious disease characterised primarily by respiratory signs in growing chickens. In hens, decreased egg production and quality are often observed. Some strains of the virus are nephropathogenic and produce interstitial nephritis and mortality. The severity of IBV-induced respiratory disease is enhanced by the presence of other pathogens, including bacteria, leading to chronic complicated airsacculitis. Diagnosis of IB requires virus isolation or demonstration of viral nucleic acid from diseased flocks. Demonstration of a rising serum antibody response may also be useful. The widespread use of live and inactivated vaccines may complicate both the interpretation of virus isolation and serology findings. The occurrence of antigenic variant strains may overcome immunity induced by vaccination.