Measurements of Texture of Soils Formed from Glaciolimnic Sediments by Areometric Method, Pipette Method and Laser Diffraction Method
Abstract The aim of the research was to compare the results of texture analyses of glaciolimnic sediments deposited in the basins of ice-dammed lakes origin in north eastern Poland. The study was carried out using aerometric method, pipette method and laser diffraction method. The studied soils were classified as Haplic and Mollic Vertisol, Vertic Cambisol, and Gleyic Chernozem. The soils were formed from clayey (clay, heavy clay), loamy (loam, clay loam, sand clay loam) and silty (silt loam, clay loam) deposits. The studied soils did not contain fractions > 2.0 mm. The amounts of clay fraction (< 0.002 mm) measured by areometric and pipette methods were similar and strongly correlated. In comparison to laser diffraction method, these amounts were 3-4-fold higher. The sub-fraction of fine silt (0.02-0.002 mm) predominated in soil formations analyzed by laser diffraction method. In comparison to areometric or pipette method, the amounts of fine silt were 2-4 fold higher. Basing on the calculated sedimentological indices, it was stated that the examined soils were well sorted and the mean grain diameter (GSS) was very low and did not exceed 0.005 mm in areometric and pipette methods, and 0.011 mm in laser diffraction method for clay sediments.
Effect of variable content of organic matter and carbonates on results of determination of granulometric composition by means of Casagrande’s areometric method in modification by Prószyński
Abstract The paper discusses the issue of the admixtures’ effect on results of granulometric composition determinations by Casagrande’s areometric method in modification by Prószyński. An experiment was conducted involving preparation of samples with known granulometric composition and determination of their composition by means of the aerometric method. Three types of samples were prepared: base (control), containing organic matter, and containing carbonates. Results of the determinations showed a considerable effect of the admixture of organic matter and calcium carbonate on the accordance of determinations, depending on their percent content. The study results also confirmed known dependencies of disturbances in proportions between the sandy and silty-clayey fraction. A practical conclusion from the research is the determination of the threshold of the organic matter and carbonates percent content at which the determination should be absolutely preceded by processes of mineralisation and decalcification.
On the fractionation of β-lactoglobulin and α-lactalbumin
Abstract Existing methods for the preparation of α-lactalbumin and β-lactoglobulin are examined critically. It is proposed that the most satisfactory method for the preparation of the whey protein fraction from bovine milk is (NH 4 ) 2 SO 4 fractionation. A number of new gentle procedures for the fractionation of the whey proteins are compared with some existing methods. The distribution of total protein at various stages is determined by total Kjeldahl nitrogen and the kind of protein by starch-gel electrophoresis. The effect of variables, such as pH, salt concentration and temperature on the separation of α-lactalbumin and serum albumin from β-lactoglobulin is studied. Differences in the behavior ( e.g. solubility) of genetic variants of β-lactoglobulin are noted. The solubility of each of the major whey proteins, under similar conditions to those used in the fractionation, is compared with its solubility in the whey protein mixture. The understanding gained of factors important in the fractionation is used in recommending new procedures for α-lactalbumin and β-lactoglobulin isolation.