GC-MS ANALYSIS AND ANTICANCER ACTIVITY OF METHANOL EXTRACT OF LEAVES OF HYPERICUM HOOKERIANUM WIGHT & ARN
Objective: The present investigation was an attempt to study the anticancer activity of the methanol extract of the leaves of H. hookerianum. GC-MS analysis revealed that the methanol extract of leaves contained important secondary metabolites viz. oxygenated hydrocarbons and phenolic hydrocarbons. These phytochemicals w e r e p r e s u m e d responsible for various pharmacological actions like antioxidant, and anticancer activities. Cytotoxic changes observed in MCF 7 cell line by the treatment of methanol extract included cell aggregation, cell rounding and cell death. The overall result indicates that the methanol extract of leaves of H. hookerianum showed promising baseline information for its potential use as an anti cancer agent.
Antitumor Activity of the Methanol Extract of Hypericum hookerianum Stem Against Ehrlich Ascites Carcinoma in Swiss Albino Mice.
A large number of plants belonging to the Hypericum family are known to possess strong antitumor properties. The methanol extract of H. hookerianum Wight and Arnott stem (MEHH) exhibited potent in vitro cytotoxic activity against various cancerous cell lines. In the present study, the high performance liquid chromatography (HPLC) standardized MEHH was tested for in vivo antitumor properties against Ehrlich ascites carcinoma (EAC) tumor bearing mice at 100, 200, and 400 mg/kg body weight doses given orally once daily for 14 days. The results indicate that administration of the extract not only increased the survival of animals with ascites tumor, decreased the body weight induced by the tumor burden, and reduced packed cell volume and viable tissue cell count, but also altered many hematological parameters changed during tumor progression, indicating the potent antitumor nature of the extract. Among the three doses tested, the 200 mg/kg body weight dose was found to be the most potent.
Effects of signaling topic structure on text recall
Two experiments studied effects of signaling devices (headings, overviews, and summaries) on text memory. In Experiment 1, subjects read a text with or without signals, then recalled the topics of the text. Signaling produced better memory for the topics and their organization. In Experiment 2, subjects recalled the content of the text they read, and recalls were scored for the number of accurately recalled ideas. Signals produced recalls that were better organized by text topics. Signals also influenced the distribution of recall of ideas: Subjects remembered more topics but recalled less about each accessed topic if the text they read contained signals than if it did not