Study on space charge effect in an electrostatic ion analyzer applied to measure laser produced ions.
The abundance of different ions produced by laser ion sources is usually analyzed by an electrostatic ion analyzer (EIA). Ion current intensities in the range of several mA/cm(2) at the position of the EIA have been achieved from the laser ion source developed by the Institute of Modern Physics; this indicates that a noticeable influence of space charge effect during the ion transmission will occur. Hence, while the parameters of the EIA or the beams are changed, such as ion species, current intensity, the ions' transmission efficiency through the EIA is different, which will result in an uncertainty in the estimation of the ions' yields. Special attention is focused on this issue in this paper. Ion's transmissions through the EIA under different circumstances are studied with simulations and experiments, the results of which are consistent with each other.
Carbon beam acceleration using a simple injection method into an RFQ
Abstract For medium-heavy ions, a new injection method into a RFQ linac is proposed. Laser plasma is created by an intense pulsed laser system and directly injected into the RFQ without any focusing devices. This method may eliminate complex difficulties due to space-charge effects in the low-energy transport system. Using the TITech RFQ, a verification test has been performed. From the laser ablated carbon plasma, C 4+ and C 3+ beams were captured successfully and were accelerated to the designed energy of the RFQ. The observed total current of carbon beam reached 8 mA, which exceeded the designed current of the RFQ. This method can be easily applied to existing RFQs which are used in medical and industrial accelerator systems.
Negatively charged phospholipids trigger the interaction of a bacterial Tat substrate precursor protein with lipid monolayers.
Folded proteins can be translocated across biological membranes via the Tat machinery. It has been shown in vitro that these Tat substrates can interact with membranes prior to translocation. Here we report a monolayer and infrared reflection-absorption spectroscopic (IRRAS) study of the initial states of this membrane interaction, the binding to a lipid monolayer at the air/water interface serving as a model for half of a biological membrane. Using the model Tat substrate HiPIP (high potential iron-sulfur protein) from Allochromatium vinosum, we found that the precursor preferentially interacts with monolayers of negatively charged phospholipids. The signal peptide is essential for the interaction of the precursor protein with the monolayer because the mature HiPIP protein showed no interaction with the lipid monolayer. However, the individual signal peptide interacted differently with the monolayer compared to the complete precursor protein. IRRA spectroscopy indicated that the individual signal peptide forms mainly aggregated β-sheet structures. This β-sheet formation did not occur for the signal peptide when being part of the full length precursor. In this case it adopted an α-helical structure upon membrane insertion. The importance of the signal peptide and the mature domain for the membrane interaction is discussed in terms of current ideas of Tat substrate-membrane interactions.