Gas chromatographic studies of derivatives of celluline-like substances.
Abstract Different aspects of the gas chromatographic determination of the celluline-like substances exhibiting specific cardiotonic activity are discussed. As this substances is isolated from frog skin and bladder, many impurities have to be considered. Determination of these samples, having a broad range of boiling points, was performed by means of programmed-temperature gas chromatography. Identification of unknown peaks was executed with the help of pure standard substances and retention indices.
A GENERALIZATION OF THE RETENTION INDEX SYSTEM INCLUDING LINEAR TEMPERATURE PROGRAMMED GAS-LIQUID PARTITION CHROMATOGRAPHY.
Abstract The different ways of describing peak positions on gas chromatograms are reviewed. The retention index is preferred to the theoretical nonane system and the relative retention. The equation given by Kovats for the calculation of the retention index in case of isothermal operation is transformed to a more general form to include also the case of linear temperature programmed operation. This generalized equation gives the same retention index for both ways of operation.
Lupin alkaloids from teratogenic and nonteratogenic lupins. II. Identification of the major alkaloids by tandem gas chromatography-mass spectrometry in plants producing crooked calf disease.
The major alkaloids of lupin plants producing crooked calf disease were identified by tandem gas chromatography-mass spectrometry and infrared analysis. The gas chromatography peak invariably present in teratogenic plants proved to be anagyrine. A minor but irregular contaminant of the peak was α-isolupanine. Other alkaloids present but apparently not associated with crooked calf disease were lupanine and two others that were probably epimethoxylupanine and 5,6-dehydrolupanine.