Radioprotective action of thiadiazole derivatives
Continuing previous investigations on the search for radioprotectors among the sulfurcontaining heterocycles [i, 2], we synthesized and studied the radioprotective properties of derivatives of 1,3,4and 1,2,4-thiadiazoles with various substituents: amino groups, alkyls, and mercapto groups. The following thiadiazole derivatives were investigated: 2alkylamino-5-alkyl-l,3,4-thiadiazoles and their methiodides (I-VII); 2-alkylamino-l,3,4thiadiazole-5-thiones (VIII-XII); 2-alkylamino-5-alkylmercapto-l,3,4-thiadiazoles (XIIIXVIII); 2,5-diamino-l,3,4-thiadiazole and its methiodide (XIX, XX), and 1,2,4-thiadiazoles (XXI-XXIII).
Synthesis a Series of 1,3,4-Thiadiazole Derivatives and Investigate Their Biological Activity
Eight compounds of new 1,3,4-thiadiazole biologically active for the series1,4-di(5-(4substitutedphenyldiazenyl)-1,3,4-thiadiazol-2-yl) benzene were prepared through the reaction of terephthalic acid with thiosemicarbazide .The product was coupled with substituted benzene through a diazotization reaction. The synthesized compounds (2-9) were identified using the analytical and spectral means. The biological activity for those compounds was investigated against three types of bacteria Escherichia coli (Gram negative bacteria), Enterobacter (Gramnegative bacteria) and Staphylococusaurens (Gram-negative). Keyword: Thiadiazol, biological active compounds, diazotization reaction تاقتشم نم ةديدج هلسلس ريضحت 1,3,4 ةيجوليابلا اهتيلاعف نع يرحتلاو لوزياداياث **ناملس يدهم حلاص ، *لشنش ىفطصم ، *اضرلا دبع ةليسو مسق* ءايميكلا ةفرصلا مولعلل ةيبرتلا ةيلك ىلايد ةعماج ءايميكلا مسق** بطلا ةيلك ىلايد ةعماج
HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment.
A novel fast isocratic reversed-phase HPLC method for simultaneous determination of chlorhexidine and its degradation product p-chloroaniline was developed. Zorbax SB Phenyl column (75 mm x 4.6 mm, 3.5 microm) was used for the separation. Mobile phase composed of acetonitrile and buffer solution of 0.08 M sodium phosphate monobasic containing 5 ml of triethylamine (0.5%) and adjust with 85% phosphoric acid to pH 3.0 in ratio 35:65 (v/v) pumped isocratically at flow rate 0.6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory.