Studies on the streaming transparency of red blood cell suspension. III. Factors controlling the streaming transparency of suspension.
Materials and methods are essentially the same as detailed in the foregoing paper (Nakamura et al., 1957 a and b), except for the following points. Erythrocytes suspension (0.4% o f cell concentration) : In the Exp. V the concentration of NaCl in media is varied between 0.1 and 1.0 mol gradually. Iodine crystal suspension: Thick alcohol solution of iodine crystal is poured into water stirring gently. Then minute needle-shaped crystals of iodine grow out in the liquid. Crystals of approximately the same dimension are gathered by means of decantation. The concentration of suspensoids is below 1%. Vessel: 36.7 cm of inner diameter. Scattered light measurement o f suspension : The light is received by the multiplyer through a tubelet 1 mm of diameter.
STUDIES ON THE STREAMING TRANSPARENCY OF RED BLOOD CELL SUSPENSION IV. STREAMING-LIGHT-INTERRUPTION-RATIO (S.L.I.R.) OF ERUTJRPCUTE SUSPENSION AND CONDITION OF ITS DETERMINATION
The authors (1957) have demonstrated in the previous paper that the absolute value of the degree of streaming transparency (D.S.T.) , being controlled by three factors, (1) absorption coefficient of suspensoids, (2) difference in density between suspensoids and medium and (3) curvature of cell surface, and not being yet reduced theoretically to the axial ratio a/b of suspensoid, is unfit to be considered as the index showing the spherocity of suspensoids in general. But it is true also that D.S.T. of erythrocyte suspension measured through red ray (660 mU) is in a good correlation with the morphological character of suspensoids, as was shown by Kuroda (1955), Nakamura (1956), and Nakamura and Nakayama (1958), favoured by the fact that the interruption of light through the suspension is due mainly to the reflexion on and to the refraction in the erythrocytes (Nakamura and Amada, 1957). However D.S.T. varies greatly in reply not only to the concentration of solute in media, as was shown in the previous paper, but also to the variable condition of preparation of the suspension; preservation condition of erythrocytes, the concentration of erythrocytes in the suspension, time after the preparation of the suspension and so on. The present report describes experiments establishing the appropriate condition of suspension to obtain the reproducible value of D.S.T. of red cell suspension by the use of our S.T. Meter.
The distribution of 54-Mn in the enamel and dentine of the rat incisor.
Abstract The distribution of 54 Mn was studied in the lower incisors of rats. Autoradiograms show 54 Mn to be present in the enamel and dentine 5, 10 and 15 days after its administration. In the dentine, the radioisotope is first localized in a zone near to and encircling the pulp and later becomes more diffuse. Pre-existing as well as the newly formed enamel incorporates the isotope. In both enamel and dentine 54 Mn appears to be principally associated with the inorganic portion.