Vinyl-film mulch: a practice for sweet potato (Ipomoea Batatas Lam. var. Edulis Makino) cultivation to reduce nitrate leaching
Abstract Under conventional cultivation practices in Southern Kyushu, Japan, a considerable amount of nitrate-N is leached from agricultural fields into ground water. The leaching processes are accelerated by high permeability of the volcanic ash soil, high rainfall and high summer temperatures. The present work was instigated to study the possibilities of controlling nitrate-N leaching by regulating the infiltration of rain water in the soil, the source of percolation below the root zone. Nitrate-N leaching from the root zone of sweet potatoes to deeper soil layers was compared in three treatments: maximum soil surface mulching with 0.03 mm vinyl-film with holes at ridge tops to supply plants with enough soil moisture to meet the crop water requirement (maximum vinly-film mulch, MVM); applications of fertilizer containing a nitrification inhibitor dicyaniamide (DCD); and conventional cultivation practices. NPK compound fertilizer (N 8 P 5.2 K 16.6 ) containing the ammonium form of nitrogen 900 kg·ha −1 and PK compound fertilizer P 8.7 K 16.6 600 kg·ha −1 were applied in the MVM and conventional plots. DCD-fertilizer (N 15 P 6.5 K 12.5 ), maintaining the same rate of absolute N with adjustment of P and K using fused magnesium phosphate (P 8.6 ) and potassium chloride (K 41.5 ), were applied to the DCD plots. MVM increased surface run-off, resulting in least percolation among the treatments. The highest nitrate-N concentration, taken as an average for 2 years, in soil-water periodically sampled throughout the growing period at 210 cm depth below surface, was found 1.8 ppm in MVM, whereas in DCD and in conventional treatments the same was measured as 8.7 and 5.2 ppm, respectively, indicating the lowest level of nitrate leaching to be in the MVM treatment. The highest availability of mineral nitrogen in the root zone of the MVM treatment was confirmed by the highest plant-top-uptake of nitrogen, Leaf Area Index and vine growth. DCD fertilizer did not contribute to the control nitrate-N leaching. There was no significant difference in root yields among the three treatments.
Simulation of N2O emissions and nitrate leaching from plastic mulch radish cultivation with LandscapeDNDC
Radish is one of the major dry field crops in Asia commonly grown with plastic mulch and high rates of N fertilization, and potentially harming the environment due to N2O emissions and nitrate leaching. Despite the widespread use of plastic mulch, biogeochemical models so far do not yet consider impacts of mulch on soil environmental conditions and biogeochemistry. In this study, we adapted and successfully tested the LandscapeDNDC model against field data by simulating crop growth, C and N turnover and associated N2O emissions as well as nitrate leaching for radish cultivation with plastic mulch and in conjunction with different rates of N fertilization (465–765 kg N ha−1 year−1). Due to the sandy soil texture and monsoon climate, nitrate leaching with rates up to 350 kg N ha−1 year−1 was the dominant reason for overall low nitrogen use efficiency (32–43 %). Direct or indirect N2O emissions (calculated from simulated nitrate leaching rates and IPCC EFind = 0.0075) ranged between 2 and 3 kg N ha−1 year−1, thus contributing an equal amount to total field emissions of about 5 kg N ha−1 year−1. Based on our results, emission factors for direct N2O emissions ranged between 0.004 and 0.005. These values are only half of the IPCC default value (0.01), demonstrating the need of biogeochemical models for developing site and/or region specific EFs. Simulation results also revealed that changes in agricultural management by applying the fertilizer only to the rows would be an efficient mitigation strategy, effectively decreasing field nitrate leaching and N2O emissions by 50–60 %.
Immunization with Brucella melitensis Rev 1 against Brucella ovis infection of rams.
The efficacy of Brucella Melitensis Rev 1 vaccine (Rev 1) for the prophylaxis of Brucella ovis ram epididymitis was evaluated. Twenty-nine 3-month-old rams were vaccinated with 2 X 10(9) Rev 1 and 14 were revaccinated with 5 X 10(8) at 14 months of age. Six rams remained unvaccinated as a control group. All rams were challenged with 5 X 10(8) B. ovis at 21 months of age. Before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams developed testicular alterations. Genital and selected extragenital organs and lymph nodes were removed at slaughter and inoculated on selective media. B. ovis was isolated from 26.6% of the vaccinated rams, 21.4% of the revaccinated rams and 100% of control rams. Portions of epididymis, testes and vesicular glands were also used for pathological studies. More severe lesions were observed in control rams than in vaccinated ones. In conclusion, these results show that vaccination of young lambs, followed or not by revaccination, is a suitable method for the prophylaxis of B. ovis infection of rams.