An electronic system of infrared spectral data for toxicological analysis.
Abstract A large number of non-volatile poisons have been analyzed by infrared spectroscopy and spectral indices have been stored as standards. Comparison of the spectra of unknown samples with the standard spectra allows identification of the unknown samples. This comparison is carried out by computer.
Application of I.R. Spectrophotometry in Emergency Analyses
I.R. spectrophotometry has been applied in emergency toxicological analyses. Poisons and drugs are extracted into chloroform, potassium bromide is added to the extract and the solvent evaporated. Potassium bromide discs are prepared by compression and scanned in an I.R. spectrophotometer. A data bank of standard spectra has been prepared and computerized comparison of the spectral data of the sample extract with those of authentic compounds allows the identification of toxic agents in 'real time'.
Die Infrarot-Spektroskopie in der Biochemie und klinischen Chemie
IR-spectroscopy can be used generally in the field of biochemistry and clinical chemistry for the investigation of the following topics: 1. Structure elucidation and identification of compounds; 2. investigation of mixtures of compounds; 3. quantitative determination of compounds in solution.With the micro- and ultra-micro methods available at present time the investigation of amounts of substances in the range of 1 μg (in special cases 0.1 μg) is possible in solution as well as by the KBr method. In layers of 0.05 mm the compounds can be determined in aquous solution both qualitatively and quantitatively. A review of literature presents information about IR-spectroscopic work dealing with enzymes, proteins, fats, lipids, nucleic acids and their components, carbohydrates, steroids, biochemical and pharmaceutical compounds.ZusammenfassungAnhand von Beispielen wird gezeigt, daß sich die IR-Spektroskopie im Bereich der Biochemie und klinischen Chemie ganz allgemein bei folgenden Untersuchungen heranziehen läßt: 1. Strukturelle Untersuchung und Identifizierung von Substanzen; 2. Untersuchung von Substanzgemischen; 3. Quantitative Bestimmung von Stoffen in Lösungen.Die heute zur Verfügung stehenden Mikro- und Ultramikro-Methoden erlauben die Untersuchung von Substanzmengen bis herunter zu 1 μg (in Extremfällen bis 0,1 μg) sowohl in Lösung als auch durch Vermessen nach dem KBr-Verfahren. In Schichtdicken < 0,05 mm lassen sich Substanzen in wäßriger Lösung sowohl qualitativ vermessen wie auch quantitativ bestimmen. Eine Literatur-Übersicht informiert über IR-spektroskopische Arbeiten, die sich mit Enzymen, Eiweißstoffen, Peptiden, Fetten, Lipiden, Nucleinsäuren und deren Bestandteilen, Kohlenhydraten, Steroiden, Biochemikalien sowie Pharmazeutica befassen.