A MICROSCOPIC STUDY OF CHLORPROMAZINE (LARGACTIL) TREATED AXOLOTL LARVAE.
In previous publications (Eyal-Giladi & Eyal, 1962; Eyal & Eyal-Giladi, 1963) some of the macroscopic effects produced by chlorpromazine (CPZ) on the development and growth rate of Axolotl embryos have been reported. They include different abnormalities (microcephaly, defects in eyes, gills and pigmentation) depending on the CPZ concentration used and the length of the immersion period. In addition, larvae subjected to high CPZ concentrations and longer time of treatment proved, at the time of fixation, to be smaller than the controls, whereas larvae treated by low CPZ concentrations tended to grow bigger than the controls.

The teratogenic effect and the influence on growth being established, a microscopic examination of the same material was undertaken.

Three experimental series were run, each starting with a different initial developmental stage (Harrison's stages 10–11, 15–16 and 29–30 respectively). Specia's (Paris) CPZ chlorhydrate powder was dissolved in sterile tap water.
Effects of chlorpromazine on brain tissue respiration.
This work demonstrates that there is a biphasic effect on brain tissue respiration with respect to time interval after chlorpromazine administration to an intact animal. The first phase, lasting about 16 hours in the rat, reflects an inhibition of respiratory activity while the second, persisting about 4 days, reflects an enhancement of respiration. It is not clear at this point whether this biphasic effect is due to a peculiarity of metabolic activity or simply represents a different rate of penetration into cortical and subcortical structures. We have demonstrated that chlorpromazine can induce opposite effects on brain tissue respiration in different parts of the brain. The hemispheres including the cerebellum show an increased respiration while the midbrain and stem show a depression of respiration following chlorpromazine administration.
Mineral element analyses of various tropical forages in Guatemala and their relationship to soil concentrations
Determination de la composition minerale de differents fourrages dans 3 regions d'elevage du Guatemala et etude des relations entre la chimie des sols et la composition minerale des fourrages