PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES OF EXTRACTS OF SOLANUM XANTHOCARPUM
Antibacterial activity of various parts (stem, leaf and fruits) of solvent extracts (Petroleum ether, alcohol and acetone) of Solanum xanthocarpum against Escherichia coil, Klebsiella of Solanum xanthocarpum showed high sensitivity to Escherichia coil & less sensitivity and resistance to Bacillus cereus. In control, no inhibitory zone was observed.
Qualitative Evaluation of Phytochemicals and Antifungal Activity of “ Solanum Xanthocarpum ” ( Schrad . & Wendl . )
Solanum xanthocarpum is a well-known medicinal herb in Asia, belongs to family Solanaceae. The Solanum Xanthocarpum is reported to have good medicinal values in traditional system of medicines. This present study deals with phytochemical investigations of different parts viz., leaf, stem, root, fruit and flowers of Solanum xanthocarpum, which were collected from three different agro-climatic zones of Chhattisgarh, India. Qualitative phytochemical test indicated that plant contains alkaloids, flavonoids, tannins, steroids and saponins as well as reducing sugars. Antifungal activity of methanol extract of different parts plant of Solanum xnathocarpum were assayed against Aspergillus niger. Screening for Anti-fungal activity is carried out by Agar diffusion method. However, the agar diffusion method can be used for determination of MIC values. Inhibition zones into MIC values were found in 20μg, 60 μg and 90 μg of FT1, ST2 and FL2 respectively. Thus Solanum xanthocarpum could be considered as a potential source of natural antifungal possesses good economic as well as medicinal importance.
Dependence of testicular germ cells on hormones: a quantitative study in hypophysectomized testosterone-treated rats.
Quantitative analysis of spermatogenesis was used to investigate the dependence of testicular germ cells on hormones in Sprague-Dawley rats. Adult hypophysectomized rats were given daily injections of testosterone propionate (TP) for 30 days. Intact and hypophysectomized control rats received vehicle only. Spermatogonia were classified as undifferentiated or differentiated, using established criteria suitable for morphological identification on periodic acid-Schiff's-haematoxylin stained sections of testis. Data on cell counts showed that the undifferentiated spermatogonia may be partially dependent on TP and/or pituitary hormones. The group of differentiated spermatogonia were dependent on pituitary hormones, and TP only partially restored their number by partially protecting them from spontaneous degeneration in stages XIV to II (A3-to-Intermediate). The maturation and division of B type spermatogonia and maturation of preleptotene spermatocytes to the zygotene stage appeared to be independent of hormones. Maturation of pachytene spermatocytes was hormone dependent, and TP completely supported their development, meiotic division and spermiogenesis in the complete absence of pituitary hormones.