Spectrophotometric methods for the determination of tamoxifen citrate.
Three simple and sensitive spectrophotometric methods for the determination of tamoxifen citrate have been developed. They are based on the formation of an ion-association complex between the drug and a dye, Erioglaucine A, which is extractable into chloroform and has an absorption maximum at 625 nm (method A), oxidation with excess potassium permanganate and the determination of unconsumed permanganate using Fast Green FCF (method B), or by the formation of a coloured cobalt thiocyante coordination complex which is extracted into benzene and measured at 635 nm (method C). Beer's law limits for methods A, B, and C are 0.5-3.0 mug ml(-1), 1.0-6.0 mug ml(-1) and 100-500 mug ml(-1), respectively. No interference was observed from tableting additives and the applicability of the methods was examined by analysing tablets containing tamoxifen. The quantities determined were 99.0-100.03% of the exptected values.
Simultaneous determination of tamoxifen citrate and its E isomer impurity in bulk drug and tablets by high-performance liquid chromatography.
A new stability-indicating HPLC method that is highly selective for the separation of E and Z isomers of tamoxifen citrate [(Z)-2-[p-(1,2-diphenyl-1-butenyl)phenoxy]-N,N-dimethylethylamine citrate] is described. The method can be used to assay tamoxifen citrate and the E isomer impurity in both bulk drug and tablets. The chromatographic system consists of a 5-microns octadecyl silica column and a mobile phase composed of an aqueous phosphate buffer (pH 2.0) and acetonitrile. N,N-Dimethyloctylamine is used as an additive in the mobile phase for improving the peak shape of tamoxifen citrate. Compared with the existing official U.S. and British Pharmacopeia methods, the new method offers the following advantages: better resolution between the E and Z isomers, shorter analyses time, stability indication, and a less aggressive mobile phase that results in a longer column life.
What can students learn from studying medicine in literature?
To identify what students can learn from studying medicine and literature as part of a final‐year special study module in general practice.