Headspace solid phase microextraction – GC/C-IRMS for δ13CVPDB measurements of mono-aromatic hydrocarbons using EA-IRMS calibration
This work aims at comparing the δ13CVPDB of mono-aromatic hydrocarbons benzene, toluene, ethylbenzene and xylene isomers (BTEX) measured by elemental analyser (EA)-isotope ratio mass spectrometer (IRMS) with the δ13CVPDB measured on the same compounds by headspace solid phase microextraction – GC/C-IRMS (hSPME – GC/C-IRMS) with the final goal of using these compounds as internal standards on the latter system. The EA-IRMS measurements were done using calcium and lithium carbonate isotopic reference materials: NBS19 and L-SVEC for establishing the δ13CVPDB scale. The EA-IRMS measurements with helium dilution of a set of five reference materials (USGS40, USGS41, IAEA-CH-6, IAEA-CH-3 and IAEA-601) show systematic bias of 1‰ relative to their assigned values. This bias due to the dilution mechanism in the used ConfloII interface device could not be avoided. As the selected hydrocarbons: BTEX could not be analysed by EA-IRMS without helium dilution, their δ13CVPDB must be corrected from this observed bias using an external calibration. The CO2 gas calibrated using EA-IRMS without helium dilution, was used as an in-house reference for the δ13CVPDB measurements of the BTEX by the hSPME – GC/C-IRMS system. The comparison made between the δ13CVPDB measured on the same BTEX compounds by EA-IRMS (with external calibration) and by hSPME – GC/C-IRMS techniques showed good agreement.
Two new organic reference materials for delta13C and delta15N measurements and a new value for the delta13C of NBS 22 oil.
Analytical grade L-glutamic acid is chemically stable and has a C/N mole ratio of 5, which is close to that of many of natural biological materials, such as blood and animal tissue. Two L-glutamic acid reference materials with substantially different 13C and 15N abundances have been prepared for use as organic reference materials for C and N isotopic measurements. USGS40 is analytical grade L-glutamic acid and has a delta13C value of -26.24 per thousand relative to VPDB and a delta15N value of -4.52 per thousand relative to N2 in air. USGS41 was prepared by dissolving analytical grade L-glutamic acid with L-glutamic acid enriched in 13C and 15N. USGS41 has a delta13C value of +37.76 per thousand and a delta15N value of +47.57 per thousand. The delta13C and delta15N values of both materials were measured against the international reference materials NBS 19 calcium carbonate (delta13C=+1.95 per thousand ), L-SVEC lithium carbonate (delta13C=-46.48 per thousand ), IAEA-N-1 ammonium sulfate (delta15N=0.43 per thousand ), and USGS32 potassium nitrate (delta15N=180 per thousand ) by on-line combustion continuous-flow and off-line dual-inlet isotope-ratio mass spectrometry. Both USGS40 and USGS41 are isotopically homogeneous; reproducibility of delta13C is better than 0.13 per thousand, and that of delta15N is better than 0.13 per thousand in 100-microg amounts. These two isotopic reference materials can be used for (i) calibrating local laboratory reference materials, and (ii) quantifying drift with time, mass-dependent fractionations, and isotope-ratio-scale contraction in the isotopic analysis of various biological materials. Isotopic results presented in this paper yield a delta13C value for NBS 22 oil of -29.91 per thousand, in contrast to the commonly accepted value of -29.78 per thousand for which off-line blank corrections probably have not been quantified satisfactorily.
Die Populationsdynamik Von Canthocampt Us Staphylinus (Jurine) (Copepoda, Harpacticoida) Im Litoral Des Bodensees
The population dynamics of Canthocamptus staphylinus in the littoral zone of Lake Constance are described (fig. 2). Adult animals aestivate as cysts. The main part of the population emerges from the cysts in autumn. During the resting stage and the first spell after emerging, mortality is very low (fig. 3). Reproduction, expressed as numbers of ovigerous females, begins in November and reaches its maximum in January. The new generation becomes adult in May/June and encysts without previous reproduction. According to Sarvala (1979) it may be possible, that in littoral populations dormancy is induced by long day periods and temperatures of over 12° C (figs. 1, 2). In the profundal zone C. staphylinus also forms cysts, however, both light and temperature are not likely to induce this stage in depths of over 60 m.