In Vitro Propagation of Selected Sugarcane ( Saccharum Officinarum L . ) Varieties ( C 86-165 and C 86-12 ) Through Shoot Apical Meristem
Sugarcane (Saccharum officinarum L.) is monocotyledonous crop plant that belongs to the Poaceae family that mostly propagates through conventional methods. However, conventional propagation lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize in vitro micro propagation protocol for two sugarcane varieties (C 86-165 and C 86-12) using shoot apical meristem explants. The two varieties were cultured on MS medium supplemented with different concentrations of growth regulators on shoot initiation, multiplication and root induction stages. Analysis of variance (ANOVA) revealed that the two varieties showed statistically significant difference in their response to the various hormonal treatments with regard to the parameters measured. For initiation stage vars. C 86-165 and C 86-12 performed best on 1.0 mg/l and 0.5 mg/l of BAP, respectively. On the other hand, multiplication stage was best in MS media enriched with 2.0 mg/l BAP + 0.5 mg/l NAA and 1.5 mg/l BAP + 0.5 NAA as manifested in terms of number of shoots and shoot length per explants after four weeks of culture for vars. C 86-165 and C 86-12, respectively. With regard to root induction, best rooting response in terms of mean root number, mean root length, mean fresh weight and mean dry weight was achieved in 1/2 MS media enriched with 2.0.mg/l NAA and 1.0 mg/l NAA after four weeks of culture for vars. C 86-165 and C 86-12, respectively.
Synergetic Effect of Different Plant Growth Regulators on Micropropagation of Sugarcane (Saccharum officinarum L.) by Callogenesis
The response of different plant growth regulators on callus induction and regeneration on three sugarcane genotypes (YT-53, CP-77-400, and NSG-59) was evaluated. Different concentrations of 2,4-D alone and in combination with other plant growth regulators (Kinetin and BAP) were used for callus induction. Kinetin along with IBA, BAP and NAA were analyzed with respect to shoot induction, while NAA and IBA were used for root induction. The best callus response in terms of number of days, callus fresh weight, and frequency in YT-53 was observed on MS media provided with 2,4-D (3 mg L−1) + Kinetin (0.5 mg L−1), while in NSG-59 the best response was on MS+2,4-D (4 mg L−1) + Kinetin (0.5 mg L−1), and in CP-77400, MS+2,4-D (5 mg L−1). For shoot induction, 2 mg L−1 Kinetin was found to be the best for YT-53 and NSG-59, while 1 mg L−1 BAP was found to be the best for CP-77-400 in terms of number of days, shoot numbers, and shoot length. The best media for root induction in terms of number of days, root numbers, and root length was 1 mg L−1 NAA + 1 mg L−1 IBA for YT-53, while this was 3 mg L−1 NAA for NSG-59. The highest root frequency and maximum root length in the minimum number of days was observed in CP-77-400 on MS media provided with 2 mg L−1 NAA.
Yield comparison in sugarcane crop raised from conventional and mericlone derived seed cane
A comparative study on performance of Mericlone- derived seed cane for % sprouting, tillers/ ha, number of millable canes/ ha, cane yield/ha, CCS% and sugar yield/ha over conventional seed cane was conducted on two sugarcane clones (Co. Se. 92423 and U.P.22) following hot water therapy (HWT), Mericlone-derived seed cane gave significantly higher % sprouting (by 7.36%), cane productivity (by 32.9%), and sugar output (by 43.0%). Hot water therapy caused significant inhibition of % sprouting (by 4.5%) but its effect on other parameters was non-significant, perhaps due to component's compensation. The two varieties significantly varied in its tillering, cane productivity and sugar output/ ton cane crushed, Seed type x HWT interaction revealed that % sprouting was significantly inhibited in mericlone-derived seed cane. Seed type x variety interaction showed that mericlone - derived seed cane was significantly superior in all parameters of which U.P. 22 was better. Variety x HWT interaction inflicted significant inhibition of % sprouting in Co.Se.92423 but improved sugar recovery in case of U.P.22.