Assessment of Bacteriological Quality of Sold Meat in the Butcher Shops of Adigrat, Tigray, Ethiopia
2 Abstract: The study was carried out from November 2011 to April 2012, through which purposive sampling methodology was used to assess the bacteriological quality of sold meat in abattoir and butcher shop of Adigrat. On a total of 80 meat samples (20 samples from each stage; abattoir and butcher house of hanging meat, minced meat and refrigerated meat). The mean values of bacterial load of hanging meat, minced meat, abattoir meatand refrigerated meat were 5.5x10 , 6.5x10 , 3.2x10 and 3x10 CFU/g respectively indicating high 777 7 mean values of microbial load in the hanging meat and minced meat sales with statistically significant difference (P<0.05).Theresult also indicated that 13.75, 46.25 and 40% of the meat are acceptable,marginally acceptable and unacceptable respectively. The major bacterial pathogens isolated were Escherichia coli; Staphylococcus aurous Streptococcus species and non-lactose fermenting bacteria. Most of the isolated bacteria were susceptible for the commonly used antimicrobials.Careless handling of meat at the slaughtering places and butcher shops affect the quality of meat which indicated as the presence of contamination. Therefore, particular attention to meat hygienic should be strengthened in both butcher houses and abattoirs workers.
Bacteriological Quality Assessment of Raw Beef Sold in Sylhet Sadar
This study was conducted to assess the bacteriological quality of fresh raw beef sold in different markets of Sylhet Sadar. A total of seventy five (75) fresh raw beef samples were randomly collected from seven  major markets (Shibgonj, Mirabazar, Kazitula, Ambarkhana, Madina market, Bandar bazar and Sheikh ghat) of Sylhet Sadar. Total Viable Count of the samples ranged between 2.5 × 10 5 to 2.25 × 10 8 cfu/g. Acceptability for consumption of these samples as satisfactory, acceptable and rejected were 40, 32 and 28%, respectively. A total of 115 bacterial isolates of 5 genera were identified including gram negative Escherichia coli [15(10%)], Salmonella spp. [20(13.33%)], Klebsiella spp. [30(20%)], Enterobacter spp. [10(6.67%)] and gram positive Staphylococcus spp. [40(26.67%)]. The presence of these organisms in fresh meat from conventional beef is alarming. DOI: http://dx.doi.org/10.3329/agric.v13i2.26654 The Agriculturists 2015; 13(2) 09-16
Fertility assessment of hydatid cyst by proton MR spectroscopy.
BACKGROUND
Hydatid cysts, the larvae of the parasite Echinococcus granulosus, may lodge in any organ of intermediate hosts, namely, man and sheep. Complete cyst removal is the treatment of choice; however, spillage of fertile cysts during surgery leads to disease recurrence that may be prevented by preoperative detection of the fertility status of the cyst. With this perspective, ex vivo proton (1H) MR spectroscopy of hydatid fluid of human and sheep origin was performed to differentiate fertile from sterile cysts on the basis of their metabolite pattern.


PATIENTS AND MATERIALS
Cysts of sheep and human origin were used as source of hydatid fluid. A fraction of this fluid was tested for cyst fertility and the rest was used for ex vivo1H spectroscopy. Histopathology of the cyst wall was done as a gold standard for this study.


RESULTS
Of 10 sheep samples, 7 were fertile and 3 were sterile, while among 6 human samples, 5 were fertile and 1 was sterile. Spectroscopic and histopathological results corroborated each other. The fluid from microbiologically proven fertile cysts contained malate and fumarate along with other resonances and the histopathology of the fertile cyst wall demonstrated germinal lining and protoscoleces.


CONCLUSIONS
The ex vivo spectroscopic differentiation of fertile and sterile cysts may be a stepping-stone for their in vivo separation in future and thus help in framing strategies for percutaneous/surgical management.