A study of the alkaloids ofVeratrum lobelianum
Conclusions1) At the beginning of vegetation, the total alkaloids in the epigeal part ofVeratrum lobelianum is 2.5% of the weight of the dry raw material, and at the end of vegetation, 0.036%.2) The new alkaloids veralosine, veralosinine, and veralosidine have been isolated from the epigeal part of the plant.3) On the basis of a study of chemical properties, IR, UV, NMR, and mass spectra, and also its conversion into tetrahydrosolasodine, structure and configuration I has been proposed as the most probable for veralosidine.
Structure of a new ester-alkaloid from Veratrum grandiflorum (Maxim.) Loesener fil. (Alkaloids of Japanese Veratrum genus plants. IV.).
A new ester-alkaloid isolated from the rhizomes of Veratrum grandiflorum (MAXIM.) LOESENER fil., m.p. 235°, [α]15D-10.9°, was found to be 16-angelate of the alkamine which had been obtained from Veratrum oxysepalum TURCZ. and characterized by Shimizu as zygadenilic acid δ-lactone.
HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment.
A novel fast isocratic reversed-phase HPLC method for simultaneous determination of chlorhexidine and its degradation product p-chloroaniline was developed. Zorbax SB Phenyl column (75 mm x 4.6 mm, 3.5 microm) was used for the separation. Mobile phase composed of acetonitrile and buffer solution of 0.08 M sodium phosphate monobasic containing 5 ml of triethylamine (0.5%) and adjust with 85% phosphoric acid to pH 3.0 in ratio 35:65 (v/v) pumped isocratically at flow rate 0.6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory.