Study on preparing fatty acid by hydrolyzing Jatropha Curcasl Oil with lipase
Used tween-80 as emulsifier, industrial lipase as catalyst to hydrolyze Jatropha Curcasl Oil and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants as follows: concentration of catalyst 4% (W/W), weight ratio of oil to water 1:4 (W/W), temperature at 40°C, and hydrolysis time is 32h. Under these conditions, the AV(acid value) of hydrolyzed rapeseed oil reached 163.2 mg KOH/g, and hydrolytic rate arrived at 89.6%.
Research Status of Biodiesel Production by Enzymatic Method and Its Prospect
Non-renewable energy sources are declining. As a renewable energy and friendly to the environment, the technology of lipase catalyzed synthesis biodiesel has received extensive attentions. This paper reviews the sorts of lipase, resources of materials, and the methods of lipase use. Meanwhile, it points out the tendency of the research and development of lipase catalyzed synthesis biodiesel. It also provides ideas of biodiesel in the future.
Investigations on the rôle of organic matter in plant nutrition
1. As the result of decomposition of green manure in a swamp soil, the coarse fractions (including coarse and fine sand) of the surface layer (first 3 inches) decreased by 11·2 per cent. while the fine ones (silt+clay) increased by 9·8 per cent. in the course of 43 days. The effect was less pronounced in the manured soil maintained at 60 per cent. saturation, while no alteration was to be noticed in the case of the unmanured specimens. 2. Treatment with dilute hydrochloric acid prior to dispersion had no appreciable effect on the estimates of mechanical composition obtained in the different cases. Loss in solution was also comparatively small. 3. The silica-sesquioxide ratio increased slightly as the result of decomposition of green manure in swamp soil. The composition of the fine fractions was also altered, the total percentages of the oxides of iron, aluminium and silicon increasing from 90·5 to 99·3 in the course of 71 days. 4. The practical significance of the above and related observations has been discussed.