Phytoecdysones ofRhaponticum integrifolium III. Integristerone B
SummaryFrom the flower heads ofRhaponticum integrifolium we have isolated a new phytoecdysone — integristerone B. It has been shown that it is 1β, 2β, 3β, 5β, 14α, 20R, 22R, 25-octahydroxy-5β-cholest-7-en-6-one.
Insect Moulting Hormones: Dacrysterone, a New Phytoecdysone From Dacrydium intermedium
Dacrysterone, a new phytoecdysone, has been isolated from the bark of Dacrydium intermedium and shown to be 5β-hydroxymakisterone A (6). Makisterone A also occurs in this plant.
Immunization with Brucella melitensis Rev 1 against Brucella ovis infection of rams.
The efficacy of Brucella Melitensis Rev 1 vaccine (Rev 1) for the prophylaxis of Brucella ovis ram epididymitis was evaluated. Twenty-nine 3-month-old rams were vaccinated with 2 X 10(9) Rev 1 and 14 were revaccinated with 5 X 10(8) at 14 months of age. Six rams remained unvaccinated as a control group. All rams were challenged with 5 X 10(8) B. ovis at 21 months of age. Before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams developed testicular alterations. Genital and selected extragenital organs and lymph nodes were removed at slaughter and inoculated on selective media. B. ovis was isolated from 26.6% of the vaccinated rams, 21.4% of the revaccinated rams and 100% of control rams. Portions of epididymis, testes and vesicular glands were also used for pathological studies. More severe lesions were observed in control rams than in vaccinated ones. In conclusion, these results show that vaccination of young lambs, followed or not by revaccination, is a suitable method for the prophylaxis of B. ovis infection of rams.