Isolation of keratomycetes from the soil of wild animal cages and enclosures in the zoo of the Parco Nazionale d'Abruzzo, Italy.
The keratinophilic fungi present in the soil of wild animal cages and enclosures in the zoo of the "Parco Nazionale d'Abruzzo", at Pescasseroli, Italy were studied. The goal of the investigation was to determine what species of such fungi existed in wild animal habitats in that area and what variation there may be in their frequency in connection with the seasons. The most prevalent fungus was Trichophyton ajelloi, followed in decreasing order by the Chrysosporium species (C. keratinophilum, C. tropicum, C. state of Ctenomyces serratus). The Microsporums were relatively rare. The perfect forms Arthroderma uncinatum, A. quadrifidum, Nannizzia cajetani and Ctenomyces serratus were isolated from plates containing their corresponding imperfect forms. The recurrence of the species present in the soil of each cage and enclosure in April and in July was remarkable.
Verbreitung keratinophiler Bodenpilze im Dresdener Raum in Abhängigkeit vom pH‐Wert des Standortes
350 Proben oberflächiger Bodenschichten verschiedener Standorte aus dem Dresdener Stadtgebiet und seiner ländlichen Umgebung im Umkreis von 10–15 km wurden auf die Verbreitung keratinophiler Bodenpilze untersucht. Für 60 Proben wurde der pH‐Wert elektrometrisch bestimmt und der Glühverlust ermittelt. Bei der Befundauswertung stand der Vergleich zwischen der Pilzbesiedlung gleicher Standorte innerhalb des Dresdener Raumes und darüberhinaus mit denen des Berliner Terrains (Böhme) im Mittelpunkt des Interesses. Verglichen wurden die Besiedlungsquote und ‐dichte sowie das Artenspektrum eines Standortes.
Mode of action of bredinin with guanylic acid on L5178Y mouse leukemia cells.
Moderate concentrations of bredinin (1.2 X 10(-5) M) strongly inhibited growth of L5178Y cells, with the effect being reversed by guanylic acid (GMP). However, at higher concentrations of breeding the inhibition was not reversed completely by GMP added in excess. Bredinin was cytocidal at concentrations above 2 X 10(-5) M, but 5 X 10(-5) M bredinin in the presence of excess GMP, bredinin was cytostatic. Bredinin inhibited nucleic acid synthesis of L5178Y cells, but bredinin itself was not incorporated into the nucleic acid. Inhibition of nucleic acid synthesis was clearly reversed by GMP. Similarly chromosomal aberrations in L5178Y cells caused by bredinin were reversed by GMP. In contrast, the effect of ahigh concentration of bredinin on cell multiplication was not reversed by GMP. The modal volume of L5178Y cells increased during incubation in the presence of bredinin and GMP for 24 hours, 5 X 10(-5) M bredinin with GMP causing a 70% increase in cell volume. This increase in cell volume was mainly due to an increase in the protein content of the cells. The cytostatic effect of bredinin with GMP was reversed completely by adenosine-3',5'-cyclic monophosphate (cyclic AMP). Other cyclic nucleotides and nucleotides were ineffective. The reversing effect of cyclic AMP on cell survival depended upon the concentration of GMP, and was not seen in the absence of GMP. It was concluded that cyclic AMP influences the secondary cytostatic effect of bredinin, and not the primary cytotoxic effect reversed by GMP.