Flavonoids ofGoebelia pachycarpa
The following substances were isolated and were identified on the basis of chemical and spectral characteristics: kaempferol, quercetin, and genistein. A fourth compound C26H2sO14 (I), mp 125-128°C, [a]~ 2 --34.4 ± 2 ° (c 1.05 MeOH) was an isoflavone derivative according to its UV spectrum (%maxethanol, nm: 263, 328 sh; log g 4.56, 3.73) [i]. Its IR spectrum contained absorption bands at (cm -I) 3527-3295 (OH groups), 1660 (C=O of a a-pyrone), 1622, 1589 (aromatic ~ bonds), and 1098-1040 (C O vibrations of glycosides).
Stoffwechselprodukte von Mikroorganismen 6‐Chlorgenistein und 6,3′‐Dichlorgenistein. 164. Mitteilung
Metabolites from Microorganisms, 6-Chlorogenistein and 6,3′-Dichlorogenistein



A strain of Streptomyces griseus produced chlorinated metabolites in media containing soybean meal. Two of these metabolites were isolated together with a chlorine free compound which could be identified as the isoflavone genistein (I) by its spectral properties and by comparison with an authentic sample. By NMR. and mass spectroscopy the chlorinated compounds were shown to be 6-chlorogenistein (II) and 6,3′-dichlorogenistein (III).



The 7-glucoside of genistein is a constituent of soybean meal; it is hydrolysed and chlorinated by Streptomyces griseus.
The peculiar thermo-structural behavior of the anionic lipid DMPG.
Aqueous dispersions of the anionic phospholipid dimyristoyl phosphatidylglycerol (DMPG), around 100 mM ionic strength, are known to exhibit a thermal behavior similar to that of the largely studied lipid dimyristoyl phosphatidylcholine (DMPC), which undergoes a gel to liquid crystalline phase transition at 23 degrees C, well characterized by differential scanning calorimetry (DSC), and other methods. However, at low ionic strength, DMPG has been shown to present a large gel-fluid transition region, ranging from 18 to 35 degrees C. This intermediate phase is optically transparent and characterized by a continuous change in membrane packing. Structural properties of the DMPG gel-fluid transition region will be discussed, based on results obtained by several techniques: electron spin resonance (ESR) of spin labels at the membrane surface and intercalated at different depths in the bilayer; light scattering; DSC; small angle X-ray scattering (SAXS); and fluorescence spectroscopy of probes in the bilayer.