A METHOD TO SCREEN WEED-SUPPRESSING ALLELOCHEMICALS IN FLORIDA BIOMASS
Wood chip mulches derived from woody species grown in Florida are sometimes used for weed suppression in perennial fruit crops, especially in sustainable and organic production systems. A method for testing allelopathic qualities of these materials was developed, including wood chip incubation and filtering followed by bioassays of lettuce seed germination and growth. Eluates of wood chips from red maple ( Acer rubrum cv. ‘ Rubrum ’ L.), swamp chestnut oak ( Quercus michauxii Nutt.), red cedar [ Juniperus silicicola (Small) E. Murray] and sweet bay ( Magnolia grandiflora L.) highly inhibited germinating lettuce seeds, as assessed by germination percentage, radicle and hypocotyl growth. The effects of eluates from these four species were more than or equal to that found for eluates from wood chips of black walnut ( Juglans nigra L.) a species with a high level of allelopathic activity. In the United States annual estimated waste biomass of urban tree and wood mill residues totaled 38,000 and 5,600 dry metric tons, respectively (National Research Council, 2000). Wood chip mulches from such biomass applied as a preor post-plant treatment have been shown to suppress weeds (Autio, 1999), moderate soil temperature (Harris, 1983), soil moisture (Russel, 1939), and increase growth of young pecan trees (Foshee et al., 1996; Smith et al., 2000). Experiments to determine allelopathic activity of woody species and their derived materials have included interplanting corn with black walnut (Jose et al., 1995) and application of plant tissue extracts to weeds growing in the field (Al-Saadawi and Al-Rubeaa, 1985; Al-Saadawi et al., 1985). Other laboratory studies assessing allelopathic activity of cover crops have used germinating lettuce seeds as a test crop because of its high sensitivity to bioactive substances (Fujii and Shibuya, 1992). Our objective was to test a laboratory bioassay for allelopathic chemicals from wood chips of species commonly found in woody biomass waste management operations. Once developed, such a laboratory bioassay could be used to evaluate biomass allelopathic potential prior to longer term field trials. This research was supported by the Florida Agricultural Experiment Station and approved for publication as Journal Series No N-02514. This research was supported by a grant from the University of Florida Center for Biomass Programs. 1 Corresponding author
Biological and Chemical Characterizations of Allelopathic Potential of Diverse Accessions of the Cover Crop Sunn Hemp
ABSTRACT. There has been increasing interest in recent years in sunn hemp (Crotalaria juncea), as a leguminous cover crop and green manure, for weed and pest management and improving soil health. Aqueous extracts and ground shoot tissue have previously been demonstrated to be phytotoxic. To further explore its allelopathic potential, bioassays and chemical characterization of water-soluble eluates of sunn hemp were undertaken. Lettuce (Lactuca sativa) radicle growth inhibition was conducted with aqueous eluates from thinly sliced sunn hemp leaves, stems, and seeds, and all three tissues exhibited the inhibitory potential. Fourteen accessions originating from the United States, India, Brazil, South Africa, Pakistan, and Nigeria had water-soluble allelochemicals in leaves, suggesting that allelopathic potential is widely distributed in this species. The highest level of inhibitory potential was found in accession IN-86. Further characterization of IN-86 leaf eluates indicated that the inhibitory compound(s) was/were not soluble in chloroform, but was/were stable when boiled for 15 minutes and resistant to 1 N HCl. Binding and elution from AG-1(OH) ion-exchange resin also were observed. An analysis of leaf eluates of IN-86 using highperformance liquid chromatography (HPLC) followed by mass spectrometry (MS) showed the presence of a compound with a mass-to-charge ratio of 148, consistent with the spectrum for hydroxynorleucine, a phytotoxic nonprotein amino acid previously reported in seeds of C. juncea. However, its low concentration (<1 mg mL) suggested that other components of the eluate were responsible for the observed allelopathic effect. The results indicate the feasibility for development of weed control strategies using allelochemicals derived from sunn hemp biomass of select genotypes IN-86, NG-71, and BR-20 from India, Nigeria, and Brazil, respectively.
Altered Fruiting and Growth Characteristics of ‘Delicious’ Apple Associated with Irrigation Method1

‘Redspur Delicious’ apple trees (Malus domestica Borkh) on seedling roots were grown from planting with water applied 1) daily by trickle irrigation in amounts determined by water use from companion trees grown in lysimeters, 2) daily by overtree sprinkler irrigation in amounts determined from daily pan evaporation, and by 3) overtree sprinkling every 2 weeks in amounts needed to return soil moisture to field capacity. Trees grown with trickle irrigation flowered and set fruit in their 3rd and 4th seasons. Most of the sprinkled trees had not flowered by their 4th year. The average length of shoot growth was less, but more buds broke on the trickle-irrigated trees resulting in equivalent total shoot growth on all treatments.