Haemolysis Effect Estimation of Doxycycline Hyclate 150 mgDelay Release Tablet in Bio-analysis (Human Plasma) by LiquidChromatography-Tandem Mass Spectrometry
A fast, simple and particular method for estimation of Doxycycline in healthy human plasma was validated using Minocycline as IS. The analyte and IS were extracted from plasma using SPE. The compound was estranged on a RP column with an isocratic mobile phase consisting of 0.1% formic acid in water and acetonitrile (12:88, v/v), and detected by tandem mass spectrometry in positive ion mode. The ion transition recorded in several reaction monitoring mode were m/z 294.1→225.1 for Doxycycline and m/z 286.1→217.1 for IS. Linearity in plasma was observed over the concentration range 0.3-30 ng/mL for Doxycycline. The mean recovery for Doxycycline was 83.7%, with a lower limit of quantification of 0.3 ng/mL. The coefficient of variation of the assay was less than 6.8%, and accuracy of 96.1% to 102.2%. The validated method was applied to bioequivalence study of 150 mg Doxycycline Hyclate tablet in healthy human volunteers. The validated method was used to expose study samples of bioequivalence study of 150 mg Doxycycline Hyclate delay release tablet in 36 healthy human volunteers. Total 50 samples from individual volunteers identified as Haemolyzed, which were analyze initial and repeat again to cross check the method reproducibity for Haeamolysis effect and compared which found acceptable range. Bioequivalence was prove for test and reference using validated method to experimental samples (Figure 1).
Importance of Bioavailability in the Pharmaceutical World
Bioavailability is characterized as the rate and degree to which the dynamic fixing or dynamic moiety is consumed from a medication item and gets to be accessible at the site of activity. Guaranteeing consistency in gauges of value, viability and wellbeing of pharmaceutical items is the principal obligation of bioavailability studies. For medication items that are not expected to be consumed into the circulatory system, bioavailability [1 4] may be evaluated by estimations planned to mirror the rate and degree to which the dynamic fixing or dynamic moiety gets to be accessible at the site of activity. Examining from the purpose of sustenance, bioavailability proposes that our digestive framework is capable of extricating nutrients from a product in a shape that can be ingested into the circulation system.
Quality evaluation of chloroquine, quinine, sulfadoxine–pyrimethamine and proguanil formulations sold on the market in East Congo DR
Objective:  Drug quality may be poor in many regions of the world. Our first aim was to verify whether the dose of the active compounds in various antimalarial medicines on the market in East Congo conforms to the quality requirements of the European Pharmacopoeia (Ph. Eur.). The second aim was to check the extent to which simple methods of analysis could be used to evaluate drug quality.