Fatty acid composition of oils of various types ofOlea europaea
The oil was extracted with n-hexane in a Soxhlet apparatus [6]. The composition and relative percentage amounts of the fatty aQids in the 0ils were studied by gas-liquid chromatography on a Chromi4 chromatograph with a 4 mm × 2.5 m column f~lled with 17% of ethylene succinate on Chromaton NAW-DMCS at 196°C. The fatty acids of the oils were chromatographed in the form of their methyl esters. The peaks of the fatty acfd methyl esters were identified from their relative retention times [7].
Fatty acid composition of oleaster pulp and pit oils
Fatty acid compositions of oleaster pulp and pit oils were determined by gas chromatography in 4 samples of different varieties. Pit oils were highly unsaturated, containing >90% linoleic, oleic, and linolenic acids, as well as traces of palmitoleic acid. Saturated fatty acids consisted of palmitic and stearic acids with traces of arachidic acid. Pulp oils showed fatty acid compositions entirely different from that of pit oils. They contained 9 saturated fatty acids, C12 to C24, some of them with high quantities, up to 34.9%, of the total fatty acids. Unsaturated fatty acids, mainly oleic and linoleic, with low quantities of palmitoleic and linolenic acids composed about one-third of the total fatty acids.
Quantification of shellfish major allergen tropomyosin by SPR biosensor with gold patterned Biochips
Abstract Tropomyosin (TM), known as the major allergen in seafood, has been increasing concerns in industrialized countries. We proposed a simple, rapid and accurate analytical method using surface plasmon resonance (SPR) sensor with gold patterned biochips for detection and quantification of TM in shellfish. The limit of detection (LOD) was 1.0 μg mL−1 (S/N = 3), while the limit of quantitation (LOQ) was 2.5 μg mL−1 (S/N = 10). Moreover, the satisfactory recoveries (92.0–108.0%) was obtained for TM detection in food samples, showing the good accuracy of this method. Furthermore, the results also demonstrated that this developed method is suitable for the determination of TM from different shellfish species within 3 min. Therefore, the developed SPR sensor with gold patterned biochips will have positive significance on the rapid and accurate detection allergens in shellfish foodstuffs, related products and their production lines in the future.