Endosperm cell number in barley
Grain weight is one of the major components of grain yield and it has been suggested that in wheat, final grain weight may be a function of endosperm cell number1. If grain weight is to be increased by increasing the rate and/or duration of endosperm cell division, it is important to establish the stage of development at which maximum endosperm cell number is attained. Evers2 reported that no cell division occurred in the endosperm of wheat later than 16 days after anthesis and other workers3,4 have come to a similar conclusion using cell counts. However, biochemical analyses5 have shown that the maximum DNA content of wheat endosperm is not reached until 25 days after anthesis and both Donovan6 and Radley7 have suggested that endosperm cell division may continue until about 30 days after anthesis. We have estimated the number of endosperm nuclei in developing caryopses of barley and have examined transverse sections of endosperms of different ages. We report here that cell division in barley endosperm continues until 28–30 days after anthesis.
Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice.
Cell number and cell division activity in rice (Oryza sativa) endosperms are possibly regulated by cytokinin levels in the endosperm and its source in the roots. This study tried to find the possible correlations among them. Six rice genotypes were grown in nutrient solution. Two patterns of endosperm cell division, synchronous and asynchronous, were observed among the genotypes based on the cell division rate of superior and inferior spikelets. Contents of zeatin (Z) + zeatin riboside (ZR) were much higher than those of N6-isopentenyladenine (iP) and N6-isopentenyladenosine (iPR) in both endosperms and roots. Changes in Z + ZR levels in endosperms were significantly correlated with those in roots, and both were very significantly correlated with the cell division rate. Changes in iP + iPR contents in the roots were not significantly correlated with those in the endosperms and the cell division rate. When roots were treated with kinetin, endosperm cell number and grain weight were increased. Such enhancement was more significantly achieved by the root kinetin treatment than by spraying kinetin on leaves and panicles. The results suggest that the cell number and cell division activity in rice endosperms are regulated by cytokinin levels in the endosperm and that root-derived Z + ZR play a pivotal role.
Using Fuzzy-Delphi technique to determine the concession period in BOT projects
BOT projects are affected by several risks and uncertainties. One of the most important issues in this type of contract is to determine the length of concession period considering these uncertainties. The previous studies have developed several models to determine the concession period. However, the uncertainties are not taking into account in most of the previous works. Moreover, none of the previous researches has the capability to aggregate the opinions of different experts being involved in a project regarding the values of the uncertain input parameters. In this research, a Fuzzy-Delphi technique is implemented to determine the length of concession period considering uncertainties. Using the proposed Fuzzy-Delphi technique, the values of different uncertain factors affecting a BOT project is determined considering opinions of a group of experts. The NPV value is calculated considering the resulted aggregated values of uncertain input parameters. Finally, the concession period is determined using fuzzy approach. A case study is conducted to evaluate the performance of the proposed methodology. It is shown that the concession period is determined more effectively using the proposed approach.