Stabilization of Gas Bubbles Released from Water-Soluble Carbohydrates Using Amphiphilic Compounds: Preparation of Formulations and Acoustic Monitoring of Bubble Lifetime
The ultrasound contrast agents Echovist® and Levovist® (Bayer AG, Schering AG, Germany) are based on the release of gas bubbles from milled α-d-galactose. In diagnostic ultrasound, for this class of contrast agents, there is a need for prolonged contrast duration. To investigate if new carbohydrate compositions could prolong the lifetime of the gas bubbles, α-d-galactose was mixed with other carbohydrates or amphiphiles with varying log P. Acoustic attenuation vs. time (390 s) area under the curve (A390) and bubble half-time (t½) were used as measures of prolonged lifetime of gas bubbles. The products, to which 0.1% of a lipophilic carboxylic acid (5β-cholanic acid, behenic acid, and melissic acid) has been added, showed more than 5, 7 and 11 times enhancement of A390, respectively, compared with the reference compound 2 (RC2) corresponding to the commercial product Levovist®. The half-time t ½ of the same compounds was prolonged more than 6 times compared with RC2. A partial least square (PLS) statistical analysis confirmed that, for additives, high log P carboxylic acids lead to the highest A390. The present results bear a promise of products with a more persistent in vivo ultrasound contrast effect than the commercially available agents.
Specific determination of free fatty acid in plasma.
Abstract In view of the diversity in methods and consequently the wide spread of the normal values mentioned in the literature, we searched for a reference method for the determination of free fatty acids (FFA). In an attempt to eliminate factors interfering with titrimetric and colorimetric methods, separation with thin-layer chromatography was introduced. After detection with Nile blue and extraction of the FFA fraction, the FFA was determined as a copper complex. The method is described and explained. The normal values found are 200–800 μmoles/l with a mean of 380 μmoles/l.
Slotted Waveguide Antenna With Two Radiation Nulls
A design of a linear array antenna having two radiation nulls and otherwise omnidirectional radiation pattern is reported. A new configuration of a waveguide broad wall longitudinal slot doublet with positive and negative offsets with respect to the waveguide center line is used to design the array. This type of doublet produces two radiation nulls. Each of the null directions can be shifted at least up to plusmn25deg by proper choice of slot offset. Resonance and radiation characteristics of the proposed doublet are also presented. Finally, one linear array antenna consisting of seven doublets has been fabricated. Measurements show that the 15 dB null widths are 14.2deg and the null depths are at least 24.8 dB. Maximum gain ripple in the omnidirectional region is 1.75 dB.