Theoretical Determination of a Critical Nitrogen Dilution Curve Based on the Carrot Case Study
The objectives of the present study were to: (a) formulate a general dilution equation for a critical nitrogen percentage in a leaf (Nc); (b) monitor dilution function of carrot as a case study; (c) demonstrate applicability of digital color imaging to monitor total nitrogen (Ntot) in crops. Carrot (Daucus carota) was grown on loess soil at five Ntot application rates. Ntot weight (kg/ha) was obtained by standard laboratory analysis and by image processing. Total yield of the treatments was the sum of canopy and roots dry matter. A new logistic decay curve Nc = a/[1 + (W/W0)b] agreed with experimental data. Nc (g/kg) is the critical nitrogen level, a and b are coefficients, and W/W0 is the relative dry matter biomass. Values of Nitrogen Nutrition Index (NNI) ≥ 1.0 indicated that there was no nitrogen deficiency in treatment larger than 100% application of Ntot. The weight of nitrogen in form of multiplication of the dry leaves weight (W) and N percent is suitable for the determination of nitrogen status. The availability of image-based data for N percent is faster, timely and less expensive than that of laboratory test. Applicability of digital color camera to monitor Ntot in crops instead of laboratory test was successfully demonstrated.
Conceptual Model
The fundamentals of the conceptual model of ethno - cultural interaction are examined in the article. Ethnic needs satisfied by social communities in various spheres of vital activity make up a structural basis of the model. The author suggests considering ethnic needs through the functioning of various spheres of society, thus the needs of the ethnos are divided into separate groups – ethno - political, ethno - social, ethno - economic and ethno - spiritual ones. The article examines the bases of the normative - legal regulation of Russian - Austrian collaboration in cultural sphere, analyzes the fundamental documents devoted to bilateral agreements on various aspects of the cultural collaboration of the two countries, assesses the impact of the normative - legal basis of the collaboration on the development of the cultural dialogue. The paper con-tains recommendations concerning the further development of the documental basis of the cultural relations of the Russian Federation and the Austrian Republic.
Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. II. Alterations of in vitro lymphoproliferation and in vivo immune responses.
To determine the functional impact of alterations in lymphocyte concentrations and ratios following infection with chicken anemia agent (CAA) alone or in combination with infectious bursal disease virus (IBDV) on the immune system of young chickens, in vitro lymphoproliferation assays and in vivo responses to vaccination with several common viral agents were assessed at various time intervals post-inoculation (PI). Concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed mitogen (PWM) stimulation of splenic lymphocytes (SPL) collected from control birds could not be detected until 10-14 days PI. Infection with CAA was characterized by significantly higher PWM stimulation of SPL at 17 days PI and significantly lower PWM stimulation of peripheral blood lymphocytes (PBL) at 14 days PI, compared with uninfected controls. Concanavalin A and PWM stimulation of SPL was significantly increased in birds inoculated with IBDV alone. Lymphocytes harvested from birds inoculated simultaneously with CAA and IBDV had significantly lower responses. Effects on humoral and cell-mediated immunity following CAA and/or IBDV were determined by evaluating vaccination responses to Newcastle disease virus (NDV), fowl pox virus (FPV) and infectious laryngotracheitis virus (ILTV) during the acute phase of CAA infection (2 weeks PI). Vaccination of birds 2 weeks following CAA infection at 1 day of age resulted in decreased protection against NDV (85.7%) and ILTV (7.1%) challenge compared with protection rates in control birds (100% and 53.3% respectively). Infectious bursal disease virus infection was associated with decreased protection against NDV (60%) only. Concomitant infection at 1 day of age resulted in a greater reduction in NDV challenge protection (33.3%), slightly decreased FPV protection (87.5%), increased numbers of persistent FPV vaccination lesions and increased protection against ILTV challenge (71.4%). Vaccination of birds 2 weeks following CAA infection at 2 weeks of age resulted in slightly decreased NDV humoral antibody, development of persistent FPV vaccination lesions (17%) and increased immunity to ILTV challenge compared with control birds (83.3% vs. 66.7%). Chickens inoculated with IBDV alone displayed a more severe depression in NDV antibody titers and only a slight decrease in ILTV protection. Vaccination following concomitant infection at 2 weeks of age resulted in a higher percentage of FPV persistent vaccination lesions (39%) and greatly enhanced immunity to ILTV challenge (100%).