Effect of temperature on hatching and growth of cuban gar (Atractosteus tristoechus) larvae
Hatching success, growth, development and survival of Cuban gar ( Atractosteus tristoechus ) larvae were examined at different temperatures (26, 28 and 30 o C) up to 18 days after hatching (DAH). The time to hatching was inversely related to the incubation temperature (87, 100 and 111 h). Larval survival at the time of hatching was significantly lower at 30 o C (50.3 %), coinciding with the highest larval mortality (30.2 %). Growth rates were 1.75 mmd -1 -10.4 % d -1 at 26 o C,1.30 mm d -1 -10.2 % d -1 -1 at 28 o C and 1.40 mm d -1 -10 % d -1 at 30 o C. Three similar critical periods were identified: 0-6, 7-11 and 12-18 DAH. During the first period, a similar increase in weight and a significant increase in total length occurred, mainly at 30 o C, indicating a more efficient reconversion of the yolk reserves. Later, growth was equally slow, corresponding with the transitional period from endogenous to exogenous feeding, indicating a similar physiologic pattern regardless of incubation temperature. Weight and length increased during the last period, with the greatest increase at 26 o C in contrast with the lowest gain at 30 o C. The inflexion points of many morphometric characters and the developmental stages accelerated with the increasing temperature. Although it was impossible to determine the optimal temperature, it was evident that 26 o C favored hatching success and larval growth.
Survival, Growth, and Development in the Early Stages of the Tropical Gar Atractosteus tropicus: Developmental Critical Windows and the Influence of Temperature, Salinity, and Oxygen Availability
Alterations in fish developmental trajectories occur in response to genetic and environmental changes, especially during sensitive periods of development (critical windows). Embryos and larvae of Atractosteus tropicus were used as a model to study fish survival, growth, and development as a function of temperature (28 °C control, 33 °C, and 36 °C), salinity (0.0 ppt control, 4.0 ppt, and 6.0 ppt), and air saturation (control ~95% air saturation, hypoxia ~30% air saturation, and hyperoxia ~117% air saturation) during three developmental periods: (1) fertilization to hatch, (2) day 1 to day 6 post hatch (dph), and (3) 7 to 12 dph. Elevated temperature, hypoxia, and hyperoxia decreased survival during incubation, and salinity at 2 and 3 dph. Growth increased in embryos incubated at elevated temperature, at higher salinity, and in hyperoxia but decreased in hypoxia. Changes in development occurred as alterations in the timing of hatching, yolk depletion, acceptance of exogenous feeding, free swimming, and snout shape change, especially at high temperature and hypoxia. Our results suggest identifiable critical windows of development in the early ontogeny of A. tropicus and contribute to the knowledge of fish larval ecology and the interactions of individuals × stressors × time of exposure.
A study of the local tissue reactions in man to cortisone and compound F. VI. Histopathological studies of the local effect of compound F in normal and pathologic skin of man.
After our previous negative studies (1) of the local effect of cortisone acetate in the skin of man, a year ago, a parallel study of 17-hydroxycorticosterone-21-acetate, Compound F, was outlined. Theoretically, Compound F should be the active steroid. It was so suggested by Rothman (2), that in all probability Compound F and not cortisone would be active locally in the skin of man.