Quantitative estimation of gossypurpurin in the isolation of gossypol
It has been found that the relative amounts of free gossypol contained in the products of the extraction of defatted high-gossypol cottonseed flour by chloroform, diethyl ether, and acetone falls with an increase in the polarity of the solvents. It has been shown that, in addition to gossypol, gossypurpurin is extracted. Its highest amount (4.82%) was found in an acetone extract, and its lowest amount (2.68%) in an ethereal extract.
Isolation of Gossypol and Analysis of Phytochemicals in Seed Extract of Bt and Non-Bt Varieties of Cotton
1. Department of Botany, University College of science, Saifabad, Osmania University Hyderabad, Andhra Pradesh, India-500004. [E-mail:shekar0987@rediffmail.com] 2. Department of Chemistry, University College of science, Saifabad, Osmania University Hyderabad, Andhra Pradesh, India-500004. 3. Principal & scientist (Plant Breeding) Regional Agriculral Research Station, Nandyal, Kurnool, Dist.518503, Andhra Pradesh, India.
Genotypic diversity in field isolates of Babesia bovis from cattle with babesiosis after vaccination.
OBJECTIVE
To determine whether particular genotypes of Babesia bovis were common to field isolates obtained from cattle properties in Queensland where the B bovis vaccine had apparently failed.


DESIGN
A comparative study of polymerase chain reaction genotypes in different populations of B bovis.


PROCEDURE
Two polymerase chain reaction assays were applied to analyse DNA extracts of B bovis vaccine (K, T and Dixie strains) and 27 field isolates from 24 properties where disease outbreaks had occurred despite the use of the vaccine. To evaluate the stability of the genotypes identified, 11 of the field isolates were inoculated into experimental cattle that had either been previously vaccinated with T strain or not vaccinated.


RESULTS
No particular genotype of B bovis was responsible for the problems observed in previously vaccinated herds. None of the isolates had genotypes identical to the vaccine strains used. No geographic trends among the genotypes were observed. Isolates that originated from the same property also had different genotypes. Blood passage of the 11 field isolates in either previously vaccinated or nonvaccinated cattle did not alter the original genotype.


CONCLUSION
No particular genotypes identified by the Bv80 and BvVA1 polymerase chain reaction assays could be associated with vaccine failures.