A novel environment-induced genic male sterile (EGMS) mutant in indica rice
SummaryMale-sterile mutants were isolated from M2 and M3 generations of indica rice variety 26 Zhaizao, dry seeds of which had been exposed to 60Co-γ rays at a dose of 290 Gy. The mutants were planted in early season and ratooned in late season for two successive years for identification of fertility conversion in different growing seasons. One of the mutant lines was further observed in a growth chamber and in the field. Results showed that daily average temperature might be the major factor conditioning the male fertility conversion at a moderate daylength. The critical temperature for the male fertility conversion of the mutant grown under 12.5 h and 14.0 h daylength is about 23°C, below which the plant becomes completely male sterile. Its male fertility conversion character differs from other EGMS lines so far developed. The performance of the hybrids between the mutant and some other indica varieties demonstrated its good combining ability and its potential value in hybrid rice production. The obtained mutant line still sheds KI-stainable pollen grains under male sterilizing conditions. Nevertheless, pollen grains shed from the male sterile plants were much more vulnerable than from normal plants. At sucrose concentration below 1.5 M, the pollen grains from the mutant grown under male sterilizing conditions almost completely broke down, while above 1.5 M they became plasmolysed and shrunken. This is indicative of poor development of the membrane and walls of the pollen grains from the male sterile mutant, causing the pollen grains to be unfunctional. NBT test also clarified the abortion of the pollen grains from the mutant, which were formed in the male sterilizing environment.
In vitro mutagenesis induced novel thermo/photoperiod-sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker
A series of leaf color mutants were isolated from young inflorescenceculture of thermo/photoperiod-sensitive genic male sterile (T/PGMS) indicarice `Xinguang S'. Most mutants maintained the temperature-insensitivityfor color expression, although some of the mutants weretemperature-sensitive and expressed the mutated colors only under higheror lower temperatures. One elite mutant `R172s' with a single recessivegene controlling green-revertible xanthan marker was found to possess thesame agronomic traits, combining ability, and T/PGMS characteristics as theoriginal parent. The marker-labeled seedlings were easily distinguished fromnormal green seedlings, and their survival rate under field conditions wassignificantly lower than that of hybrid seedlings. These results suggest thatthe leaf color mutations may be used as an ideal marker for monitoring theseed purity in hybrid rice production.
The Performance of Insecticides – A Critical Review
As part of minimizing the risk of crop losses due to herbivorous arthropods (here, mainly referring to insects and mites), most of the World’s commercial food production systems are subjected to several applications of pesticides before being harvested. Some crops are sprayed 10-20 times, while most field crops are sprayed 1-5 times during the growing sea‐ son. In the US alone, the average number of insecticide applications ranges from 1-3 for most of the major field crops, and the total annual costs of pesticide applications exceed $1.3 billion ([1], U.S. Department of Agriculture agricultural statistics services: www.nass.us‐ da.gov/). Several large ag-producing countries publish extensive details on insecticide use, including Australia, and the data presented below were collected from a public website (http://usda01.library.cornell.edu/usda/nass/AgriChemUsFruits//2000s/2008/AgriChemUs‐ Fruits-05-21-2008.pdf) on pesticide applications in the US in 2006. A somewhat extreme but also important example is the fresh sweet maize production in the US, which was grown on about 85,000 ha. Based on data from 14 US states, 20 different active ingredients of herbi‐ cides were applied an average 1.04 times to fresh sweet maize and amounted to about 2.6 kg of active ingredients of herbicides per ha. The same data suggested that about 3.5 kg of 23 different active ingredients of insecticides were applied on average 2.10 times per ha. Simi‐ larly, tomato fields (grown on about 42,000 ha in the US in 2006) were treated with 12 differ‐ ent active ingredients of herbicides, which were applied, on average, 1.14 times and the equivalent of about 0.7 kg of active ingredients per ha. Regarding insecticides, the same to‐ mato fields were treated with 32 different active ingredients, which were applied an average of 3.6 times and equal to about 4.9 kg of active ingredients per ha. While tomatoes and sweet corn may be close to the top of the list of growing crops receiving pesticide treatments, cau‐ liflower, celery, and many other horticultural crops and fruits are also subjected to intensive pesticide spraying regimes. Thus, farmers acknowledge that weeds and arthropods can po‐ tentially cause significant economic losses, and total pesticide application costs are low