Analysis of Clostridium botulinum toxigenic types A, B, and E for fatty and carbohydrate content.
Lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic KOH. Gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of Clostridium botulinum toxigenic types "A," "B," and "E." C. perfringens type "A" and Escherichia coli strain "B" were also studied in a similar manner and were found to give pattern profiles which were distinct even from those obtained for the C. botulinum microorganisms. Amino sugar content of the five microorganisms was determined by using a Beckman amino acid analyzer. The molar ratio of glucosamine to that of galactosamine was found to be of further assistance in distinguishing the individual microorganisms.
Stable gas-liquid chromatography column for fatty acid analysis
A mixed gas-liquid chromatography column of FFAP and Apolar-7CP has excelelnt thermal stability and shows excellent characteristics for the separation of the methylesters of fatty acids from Salmonella enteritidis call wall lipids.
Taxonomy of Clostridium bifermentans and Clostridium sordellii III. Agglutinability of Heat-Resistant Substrains of Clostridium sordellii
Huang, C. T. (Kanazawa University, Kanazawa, Japan), Kenzo Tamai, and Shoki Nishida. Taxonomy of Clostridium bifermentans and Clostridium sordellii, III. Agglutinability of heat-resistant substrains of Clostridium sordellii. J. Bacteriol. 90:391–394. 1965.—By cross-agglutination tests with Clostridium bifermentans and C. sordellii antisera, the heat-resistant substrains from each pathogenic strain of C. sordellii were found to possess antigens of C. bifermentans in addition to displaying changes in biological activities. The agglutinability and biological activities varied with the sporulating potency acquired. Agglutinin-absorption tests further proved that heat-resistant substrains of C. sordellii were immunologically identical to C. bifermentans.