Self-incompatibility in Spanish clones of apricot (Prunus armeniaca L.) tree
The present study is about some aspects of the reproduction physiology of twenty apricot clones ( Prunus armeniaca L.) grown in Abarán (Murcia, Spain). Self incompatibility of these clones was determined not only in field conditions in terms of the fruit set by means of self fertilization and free pollination, but also in laboratory conditions, by means of fluorescence microscopy of pollen germination on the stigma and of the growth of the pollen tube towards the egg. The only clone with signs of self incompatibility was ‘Moniquí Azaraque’. The clone with the lowest fertilization time was ‘Chicanos Archena’.
Calidad de flor y cuajado de fruto en albaricoquero (Prunus armeniaca L.) cv Moniqui
[ES] Con el fin de conocer que es la calidad de flor, se ha valorado el estado nutritivo del pistilo Para ello se ha desarrollado un metodo que, mediante la combinacion de tecnicas histoquimicas y analisis de imagen, permite evaluar el contenido de almidon al microscopio. El examen secuencial de flores ha puesto de manifiesto que el pistilo se desarrolla inicialmente a partir de sus propias reservas, que se encuentran compartimentalizadas en su interior, y que siguen un patron de consumo independiente unas de otras El almidon que se encuentra en la flor en antesis condiciona de manera directa la fecundacion y el inicio de la fructificacion, y su perdida es determinante en el proceso de degeneracion del ovulo secundario. El hecho de que las reservas que presenta el pistilo en antesis influyen en la fcundacion y en La fructificacion, unido a que existen claras diferencias entre flores en cuanto a su contenido de almidon, explica el diferente exito reproductivo que se produce entre las flores y desvela que el principal determinante de la calidad de flor es su contenido de almidon.
Hapten inhibition of passive antidextran dextran anaphylaxis in guinea pigs. Role of molecular size in anaphylactogenicity and precipitability of dextran fractions.
High titred antidextran antisera have been raised in rabbits by prolonged immunization with soluble conjugates between isomaltose oligosaccharides or dextran mw 4,400 and bovine serum albumin in complete Freund’s adjuvant. Guinea pigs sensitized with such antisera exhibited anaphylactic shock on i.v. challenge with fractions of hydrolyzed B512 dextran. The severity of anaphylaxis depended on the amount of sensitizing antibody and the dose and molecular size of the challenging dextran fraction. In maximally sensitized animals, fractions of mw 3,600 or larger were anaphylactogenic and smaller molecular size fractions were nonanaphylactogenic. In submaximally sensitized animals, dextran fractions of mw 10,500 or less were foundnonanaphylactogenic. In addition, nonanaphylactogenic dextran fractions produced desensitization, i.e. protection from anaphylaxis due to subsequent or simultaneous injection of more high molecular and anaphylactogenic dextran fractions. When the molar ratio in a mixture of nonanaphylactogenic/anaphylactogenic fractions was about 7.6, protection was complete with reduction of mortality from 100 to 0%. A molar ratio of 1.9, corresponding to a 13-percent by weight admixture of nonanaphylactogenic dextran, still significantly reduced mortality from 100 to 50%. From the ability to form precipitates with antidextrans and to elicit anaphylaxis in sensitized guinea pigs, and from the absence of these properties, the approximate molecular size range of bivalent and monovalent dextran B512 fractions was found to correspond to mw 3,600 and mw 1,430, respectively. The practical applicability of hapten inhibition is discussed.