Comparing interfertility data with random amplified microsatellites DNA (RAMS) studies in Ganoderma Karst. Taxonomy
The taxonomy of the causal pathogen of basal stem rot of oil palms, Ganoderma is somewhat problematic at present. In order to determine the genetic distance relationship between G. boninense isolates and non-boninense isolates, a random amplified microsatellites DNA (RAMS) technique was carried out. The result was then compared with interfertility data of G. boninense that had been determined in previous mating studies to confirm the species of G. boninense. Dendrogram from cluster analysis based on UPGMA of RAMS data showed that two major clusters, I and II which separated at a genetic distance of 0.7935 were generated. Cluster I consisted of all the biological species G. boninense isolates namely CNLB, GSDK 3, PER 71, WD 814, GBL 3, GBL 6, OC, GH 02, 170 SL and 348781 while all non-boninense isolates namely G. ASAM, WRR, TFRI 129, G. RES, GJ, and CNLM were grouped together in cluster II. Although the RAMS markers showed polymorphisms in all the isolates tested, the results obtained were in agreement with the interfertility data. Therefore, the RAMS data could support the interfertility data for the identification of Ganoderma isolates.
Ganoderma Diseases of Perennial Crops
This history of Ganoderma taxonomy phylogeny development of detection methods Ganoderma in southern South America - an approach to its systematics status of Ganoderma on oil palm in Indonesia Ganoderma diseases of tea - current status in Malaysia status of Ganderma diseases of forest trees in India the spread of Ganoderma from infective sources in the field some insects associated with Ganoderma fruiting bodies the use of soil amendments for control potential biocontrol agents for control in the field in Indonesia breeding for resistance to Ganoderma wilt of coconut in India.
Preparation and performance evaluation of swine manure bio-oil modified rubber asphalt binder
Abstract In order to study the effect of the preparation process of swine manure bio-oil modified rubber asphalt binder on the rheological properties of the binder, the matrix asphalt and asphalt rubber were used as the control group, and swine manure bio-oil modified rubber asphalt binders were obtained by three preparation processes. Then the rheological properties of five binders were evaluated by rotational viscosity, DSR, MSCR and BBR tests. The results show that the addition of swine manure bio-oil to asphalt rubber is beneficial to the workability, but cause negative effect on its high temperature performance, fatigue performance and low temperature crack resistance. The preparation method of premixing bio-oil and rubber powder can reduce the degradation of high-temperature performance of asphalt rubber caused by addition of swine manure bio-oil to a certain extent. The fatigue properties of the three bio-binders are close, and the critical temperature is less than 25 °C. The process of premixing bio-oil and rubber powder will increase the creep stiffness and reduce the low-temperature performance. However, the low-temperature performance of the binder obtained by this process is still better than that of the matrix asphalt and can meet the requirements of use. Therefore, it is recommended to use the preparation process of premixing bio-oil and rubber powder to prepare swine manure bio-oil modified asphalt rubber.