The structure of ervinceine
UV spectrum: Xma x (ethanol) 248, 328 m/l (log ~ 4.12, 4.26), which is characteristic for a -methylene indol ine alkaloids. The NMR spectrum of ervinceine with signals at 8 8, 88 (NH, singlet), 6 .14-6 .91 (aromatic protons, 3H), [ 3.69 (singlet, COOCHs), 3.66 (singlet, OCHs), and 0.58 (triplet, --C--C~Hs). I The reduction of ervinceine with zinc in methanoiic sulfuric acid gave dihydroervinceine, C2zHz0NzOz, mp 118:119" C (methanol), [a]~3 +3.3 (c 0.9; chloroform). Its IR spectrum; Xrnax (ethanol) 249, 305 mg (log ~ 3.78, 3.73) is characteristic of ind0tine bases. Its mass spectrum has the molecular peak M + 370 (16%) and also peaks of ions with m/e 339 (M--OCH~) + I0%, 2~4 (M--86) + 26% and 124 (I00%).
Action of alkaline solutions of hydroxamic acids on guanylic acid and RNA
ConclusionsIt has been shown that at pH 10 and 37° C, hydroxamine acids do not react directly with any of the components of ribonucleic acid. Under these conditions the cleavage of the polynucleotide chain takes place at an appreciable rate.
Selective and sensitive TNT sensors using biomimetic polydiacetylene-coated CNT-FETs.
Miniaturized smart sensors that can perform sensitive and selective real-time monitoring of target analytes are tremendously valuable for various sensing applications. We developed selective nanocoatings by combining trinitrotoluene (TNT) receptors bound to conjugated polydiacetylene (PDA) polymers with single-walled carbon nanotube field-effect transistors (SWNT-FET). Selective binding events between the TNT molecules and phage display derived TNT receptors were effectively transduced to sensitive SWNT-FET conductance sensors through the PDA coating layers. The resulting sensors exhibited an unprecedented 1 fM sensitivity toward TNT in real time, with excellent selectivity over various similar aromatic compounds. Our biomimetic receptor coating approach may be useful for the development of sensitive and selective micro- and nanoelectronic sensor devices for various other target analytes.