Isolation of Abiotrophia spp. with Staphylococcus aureus in a case of mastitis in a cow.
Isolation of Streptococcus suis from a young wild boar C treptococcus suis is an important pathogen of pigs, which has also been recognized as a zoonotic agent (1). Streptococcus suis has been associated with sporadic infections in a variety of mammalian species, such as, cattle, bison, horses, sheep, goats, dogs, and cats (2). It has recently been isolated from psittacine birds, zebrafinches, canaries, bullfinches, and a duck (2). A 20-day-old, wild boar from a 30-sow herd was submitted to the Faculte de medecine vet6rinaire of the Universit6 de Montr6al with signs of pneumonia and diarrhea. The animal was alert and did not show any neurological signs. The boar was euthanized; at necropsy, the cranial lobes of the lung, particularly on the the right side, were firm textured. The caudal lobes of the lung were not affected. There was no evidence of arthritis. Microscopic examination of the lung revealed suppurative bronchopneumonia with hemorrhagic foci and fibrinous exudation. A moderate number of coccidia were seen in sections of the small intestine. Bacteriological cultures of lungs yielded Streptococcus suis capsular type 2 colonies in large numbers, as well as a comparable number of colonies of Escherichia coli. The E. coli isolated from the intestines did not possess any of the enteropathogenicity virulence factors, and tests for rotavirus and coronavirus were negative. A total of 3 litters in this herd showed signs of infection with Streptococcus suis. Marked clinical improvement was noted after pigs from these litters were injected with 300,000 units of procaine penicillin G. The origin of the infection was not determined, but there is speculation that it spread from a swine herd on the same farm.
Streptococcus defectivus sp. nov. and Streptococcus adjacens sp. nov., Nutritionally Variant Streptococci from Human Clinical Specimens
Nutritionally variant streptococci (12 strains) formed two deoxyribonucleic acid hybridization groups (as determined by the S1 nuclease method) that were distinct from 39 Streptococcus spp. type and reference strains. These two groups differed by their penicillin-binding protein patterns and biochemical properties. Streptococcus defectivus sp. nov. (with type strain SC10) and Streptococcus adjacens sp. nov. (with type strain GaD) are the names proposed for these species.
Novel method for the determination of the methoxyl content in lignin by headspace gas chromatography.
The paper reports on a headspace gas chromatographic (HS-GC) method for the determination of methoxyl in lignin. The method involves the quantitive cleavage of methoxyl with hydroiodic acid (HI) to form methyl iodide in a closed headspace sample vial at 130 °C for 30 min. After HI has been added, the sample is neutralized by injecting a sodium hydroxide solution; the methyl iodide in the vial was determined by HS-GC using a flame ionization detector. The results showed that the method has an excellent measurement precision (RSD < 0.69%) and accuracy (RSD < 3.5%) for the quantification of methoxyl content in lignin. The present method is simple and accurate and can be used for the efficient determination of methoxy1 content in lignin and related materials.