Deoxycalyciphylline B, a Hepatotoxic Alkaloid from Daphniphyllum calycinum
Daphniphyllum calycinum (DC), a main component of Chinese patent drug Fengliao-Changweikang, is effectively used to cure bowel disease in the clinic. It was recorded that DC possessed slight toxicity, which was caused by the alkaloids existing in its extract. Unfortunately, to date the toxicity level and toxic constituents are still unclear. The present study is designed to illustrate the acute toxicity and induced organ damages of the total alkaloids as well as to determine the toxic constituents. Based on the above studies, not only was the acute toxicity determined but also hepatic toxicity was characterized by increased plasma biomarkers of ALT and AST and liver cell inflammatory infiltrate as well necrosis that was firstly observed. Significantly, deoxycalyciphylline B exhibited exactly the same hepatic toxicity so it was identified as the main toxic constituent in DC. An obvious dose-effect relationship between the toxic compound and induced hepatic injuries was also observed. Moreover, the Chinese patent drug Fengliao-Changweikang contained low levels of the toxic compound, compared with the total alkaloids. Therefore, this Chinese patent drug could be regarded to be safe in this point of view.
Daphniyunnines A-E, alkaloids from Daphniphyllum yunnanense.
The first chemical study on the stems and leaves of Daphniphyllum yunnanense led to the isolation of five new alkaloids, daphniyunnines A-E (1-5). Daphniyunnines B-E (2-5) are four unusual C-22 nor-Daphniphyllum alkaloids. The structures of these alkaloids were characterized by spectroscopic methods, especially 2D NMR techniques. A single-crystal X-ray diffraction analysis was used to confirm the structure of 1. Daphniyunnine D (4) showed cytotoxicity against two tumor cell lines, P-388 and A-549, with IC(50) values of 3.0 and 0.6 microM, respectively.
Laser ignition of explosives: Effects of gas pressure on the threshold ignition energy
Abstract This paper describes a method for measuring the effect of initial gas pressure and the type of gas on the threshold ignition energy of several high explosives. The method is based on a 300 W CW CO2-laser. An extensive study of these parameters for PETN and RDX is also presented. These high explosives all show a strong decrease in ignition energy/power with increasing initial gas pressure. The gases tested are air and nitrogen. The studied pressures are in the interval 0.1 to 10 MPa. The ignitability of PETN and RDX was tested at a laser pulse width of 1.2 ms. All tested high explosives had a lower ignition energy/power in air than in nitrogen at low pressures ( 4 MPa). For TNT the time to ignition, measured as a function of air pressure, decreased with increasing pressure. These results are interpreted as a multiple phase ignition process. The method was also used for obtaining ignition data for ...