A MICROSCOPIC STUDY OF CHLORPROMAZINE (LARGACTIL) TREATED AXOLOTL LARVAE.
In previous publications (Eyal-Giladi & Eyal, 1962; Eyal & Eyal-Giladi, 1963) some of the macroscopic effects produced by chlorpromazine (CPZ) on the development and growth rate of Axolotl embryos have been reported. They include different abnormalities (microcephaly, defects in eyes, gills and pigmentation) depending on the CPZ concentration used and the length of the immersion period. In addition, larvae subjected to high CPZ concentrations and longer time of treatment proved, at the time of fixation, to be smaller than the controls, whereas larvae treated by low CPZ concentrations tended to grow bigger than the controls.

The teratogenic effect and the influence on growth being established, a microscopic examination of the same material was undertaken.

Three experimental series were run, each starting with a different initial developmental stage (Harrison's stages 10–11, 15–16 and 29–30 respectively). Specia's (Paris) CPZ chlorhydrate powder was dissolved in sterile tap water.
Growth retarding and growth promoting effect of chlorpromazine on developing Amblystoma mexicanum (axolotl) embryos
Summary Axolotl embryos were immersed in different concentrations of chlorpromazine (CPZ) for varying periods of time and then transferred into tap water, and reared up to Harrison's stage 46. From measurements of the surface area of the head of our experimental larvae at this final stage, it was concluded that low concentrations of CPZ caused increased growth. This biphasic effect of CPZ in vivo , correlates well with the biphasic effect of CPZ on isolated enzyme systems. It is suggested that this effect may involve changes in P/O ratio as well as changes in mitotic activity.
Application of 2μm Tm Laser on Biomedicine
Continuous wave Thulium laser emits at wavelength of 2.0 mm, which is well suited for tissue ablationand thermally stimulating cutaneous nerves due to its high absorption by water. 2 mm laser can be transmitted suffi-ciently by common quartz fibers and it is safe for eyes. A brief introduction of principle of 2 mm CW Tm Laser is giv-en, analyzing the photothermal modeling of thulium laser-tissue interaction, comparing 2 mm CW Tm Laser with tradi-tional laser.