Synthesis and fungitoxicity of some pyrimidine derivatives
A series of 12 pyrimidine derivatives were prepared and testedin vitro against growth, sporulation and nucleic acid content ofFusarium oxysporum f. sp.lycopersici andHelminthosporium oryzae. Introduction of a thiazole ring together with two aryl groups into 2-aminopyrimidine brought about drastic toxicity for both fungi. Pyrimidine derivatives with aryl groups alone were less toxic. Nitro groups were found to enhance the toxicity of the pyrimidine derivatives especially when substituted in the ortho-position of the aryl groups. Inhibition of nucleic acid synthesis of both fungi was attributed mainly to the presence of the thiazole ring.
Synthesis of Novel Acyclic Nucleoside Analogue Starting From 6-Aminouracil as Potent Antimicrobial Agent
Abstract 6-Aminouracil and 2-bromoethyl amine were prepared, as starting materials to be introduced as an alkylating reagent with sodium carbonate as a catalyst. Acyclic nucleoside was prepared for the first time, the expected structure of the final new compound 3 was determined based on IR, NMR, and mass spectroscopy, with safe and mild reaction conditions. The synthesized acyclic nucleoside has a potent and efficient antimicrobial activity compared to reference drugs particularly as an antibacterial agent, and can be used as an alternative to the commonly used antibiotics after performing the necessary biological research for its validation. Graphical Abstract
Changes in Fungi with Age I. Chemical Composition of Rhizoctonia solani and Sclerotium bataticola
Gottlieb, David (University of Illinois, Urbana), and James L. Van Etten. Changes in fungi with age. I. Chemical composition of Rhizoctonia solani and Sclerotium bataticola. J. Bacteriol. 91:161–168. 1966.—The chemical composition of the mycelium of Rhizoctonia solani and Sclerotium bataticola was determined in cells of various ages. The percentage, per unit of dry weight, of soluble amino nitrogen, deoxyribonucleic acid (DNA), ribonucleic acid (RNA), ergosterol, and protein decreased with age in both fungi. Total lipids and fatty acids increased with age in S. bataticola but remained constant in R. solani. Total carbohydrate increased with age in R. solani and decreased in S. bataticola. Fewer changes with age were observed when the results were calculated in ratio to DNA. There was no change in the ratios of protein, RNA, and soluble amino nitrogen to DNA with age in either fungus, but the ergosterol-DNA ratio decreased. The total lipid-DNA ratio and the total fatty acid-DNA ratio increased with age in both fungi, whereas the total carbohydrate-DNA ratio increased in R. solani but remained constant in S. bataticola. Both fungi contained myristic, palmitic, palmitoleic, stearic, oleic, and linoleic acids. In addition, R. solani contained pentadecanoic acid, and S. bataticola had myristoleic, linolenic, and arachidic acids. No marked change in the fatty acid pattern of S. bataticola was observed with age, whereas in R. solani the percentage of linoleic acid per total fatty acids decreased slightly when oleic acid increased.