A novel Salvialactomine from the callus culture of Salvia santolinifolia Boiss
Abstract A novel compound Salvialactomine (1) along with two other unusual occurring natural products Pentatriacontanoic acid 1, 3-dihydroxypropyl ester (2) and 5-Methylflavone (3) were isolated from the callus of Salvia santolinifolia Boiss. Callus was initiated on MS medium containing NAA (0.5 mg/L) and further sub-cultured on MS medium supplemented with NAA with BA (0.5 + 1.5 mg/L). The structures of isolated compounds were determined by using mass spectrometry, 1D, and 2D–NMR techniques. Compounds 1, and 3 were tested for two different cancer cell lines, i.e. Hela (Cervical cancer cell) and PC-3 (Prostate cancer cells). IC50 was found as > 30 using Doxorobicin (0.912 ± 0.12 μmol L−1) as a standard.
In Vitro Mass Multiplication and Isolation of Natural Products from Callus Tissues of Salvia santolinifolia ( Boiss )
Method for mass multiplication of callus of Salvia santolinifolia by nodal explants was investigated by optimizing the concentration and combinations of different plant growth regulators in MS medium. Nodal explants could be stimulated to form callus on MS medium supplemented with 1-Naphthalene acetic acid (NAA) (0.1, 0.5 and 1.0 mg/l). Large scale multiplication of callus was achieved on MS medium containing NAA (0.3 mg/l) plus N Benzylaminopurine (BAP) (1.5 mg/l) and NAA (0.3 mg/l) plus N-(2-isopentyl)-adenine (2iP) (1.0 mg/l). The phytochemical investtigation of callus lead to the isolation of β-Sitosterol and Stigmasterol compounds.
Phytochemical and FT-IR Spectral Analysis of Certain Bamboo Species of SouthIndia
Bamboo with its varied uses has historically been an integral part of livelihoods and lifestyles in India. The present study was carried out to characterize the bioactive constituents present in the leaf extracts of certain bamboo species using Fourier transform infrared spectroscopic analysis (FT-IR). The various solvent extracts of various bamboo leaves subjected to qualitative analysis showed the availability of phytoconstituents including alkaloids, phenolic compounds, flavonoids, saponins, glycosides, terpenoids, steroids, coumarins and phytosterols. Quinones were completely absent in all the five bamboo species. The FT-IR spectrum confirmed the presence of sulphides (SS), phenols (C-O), aromatic compounds, thiols (S-H), aliphatic compounds (C-Cl) and amines (NH) in different bamboo species. The present investigation has also opened avenues for further research into the development of potent phytomedicines from different bamboo species.