Characterization of chitinolytic bacteria and histological aspects of Shell Disease Syndrome in European spiny lobsters (Palinurus elephas) (Fabricius 1787).
The present research reports the first description of Shell Disease Syndrome in European spiny lobsters Palinurus elephas (Fabricius 1787), which occurred in an experimental aquaculture facility in Sicily (Italy). Both bacterial characterization and histopathological examination of the exoskeleton at site of lesions was carried out. Infected specimens showed tail fan erosions, and in one case uropod ulceration and complete loss of periods. Identified species included: Listonella anguillarum 50.5%, Vibrio parahaemolyticus 27.5% and Vibrio alginolyticus 22%. Microscopic evaluation of lesions indicate the presence of inflammatory responses, which include melanization and pseudomembrane formation, similar to those described for other crustaceans affected by SDS.
A novel bacterial disease of the European shore crab, Carcinus maenas molecular pathology and epidemiology.
Several rickettsia-like diseases have been reported in arthropods (insects and crustaceans), some of which result in significant losses of economically important species such as shrimp and crabs. This study reports on the molecular pathology of a recently emerged disease of the European shore crab, Carcinus maenas, termed milky disease - named as a result of the unusual milky appearance of the haemolymph (blood). This disease was more prevalent (>26 %) during summer months when the water temperature in a pilot crab farm was approximately 19 degrees C. The putative causative agent of the disease was a Gram-negative bacterium that could not be cultured on a range of agar-based growth media. Diseased crabs showed significant reductions in free blood cell numbers and total serum protein. Such animals also displayed raised levels of glucose and ammonium in blood. Ultrastructural and in situ hybridization studies revealed that the causative agent associated with milky disease multiplied in the fixed phagocytes of the hepatopancreas (digestive gland), ultimately to be released into the haemolymph, where the circulating blood cells showed little response to the presence of these agents. Attempts to induce the infection by short-term temperature stress failed, as did transmission experiments where healthy crabs were fed infected tissues from milky disease affected individuals. Sequence analysis of the 16S rRNA gene from the milky disease bacteria indicated that they are a previously undescribed species of alpha-proteobacteria with little phylogenetic similarity to members of the order Rickettsiales.
Woodrow Wilson: The Essential Political Writings
Chapter 1 Preface Chapter 2 Introduction to Wilson's Political Thought Part 3 Progress and the Organic State Chapter 4 The State (chapters 1, 2, 13, 16) Chapter 5 "Christ's Army" Chapter 6 "Christian Progress" Part 7 Political Liberty and the Scope and Ends of Government Chapter 8 "Socialism and Democracy" Chapter 9 "A Calendar of Great Americans" Chapter 10 "Address on Thomas Jefferson" Chapter 11 "The Authors and Signers of the Declaration of Independence" Chapter 12 The New Freedom (chapters 1, 2) Part 13 Institutional Reform Chapter 14 "Cabinet Government in the United States" Chapter 15 Congressional Government (chapter 2 & conclusion) Chapter 16 Constitutional Government in the United States (chapters 3, 8) Chapter 17 "Wanted - A Party" Part 18 Political and Administrative Leadership Chapter 19 "Leaders of Men" Chapter 20 "The Study of Administration" Part 21 Foreign Policy Chapter 22 "War Message to Congress" Chapter 23 "Fourteen Points" Part 24 Appendix A. Chronology of Wilson's Academic Work and Other Selected Events Part 25 Appendix B. Selected Bibliography