COLUMNAR APPLE TREES AND THEIR VARIETIES
DOKOUPIL, L., ŘEZNICEK, V.: Columnar apple trees and their varieties. Acta univ. agric. et silvic. Mendel. Brun., 2012, LX, No. 8, pp. 37-48 The objective of the study was to evaluate selected varieties of columnar apple trees. The trees were monitored over a 3-year period and the focus was on growth, yield and health data. At the same time the quality of the harvested fruit was evaluated aer short storage. The varieties Polka, Bolero, Charlotte, Mc. Intosh Wijcik, Waltz and the control variety Golden Delicious were grown on rootstock M 9. The decisive data characterising the variety were: the height of the elongation, formation of the lateral fruiting spurs, number of fl owers and the amount of harvested fruit. No less important for growth was the health condition of the leaf area and particularly the incidence of fungus diseases; here the monitoring was carried out on six dates and was focused on apple scab and powdery mildew of apple. Infestation of the variety Charlotte with powdery mildew of apple was signifi cantly heavier than infestation of the other varieties. The variety Charlotte was also signifi cantly more aff ected with apple scab and diff ered from the other less infested varieties. The highest number of ripe fruit was produced by the variety Polka; signifi cantly less fruit was produced by the varieties Bolero, Mc. Intosh Wijcik and Waltz. The least number of fruit but the heaviest was produced by the variety Charlotte; the weight of the fruit of this variety was signifi cantly the highest. The variety Polka gave the highest yields. Evaluations of the organoleptic qualities included the appearance of the fruit; highly appraised in this respect was the control variety Golden Delicious, followed by Bolero and Mc. Intosh Wijcik. In terms of the appearance the variety Waltz received the lowest scores. The varieties Mc. Intosh Wijcik and Charlotte had the thickest skin, while the skin of varieties Polka, Golden Delicious and Waltz were of no concern. In terms of the consistence of the fl esh the highest- ranking variety was Golden Delicious, followed by Bolero and Charlotte. The juiciest variety was Golden Delicious, followed by Mc. Intosh Wijcik and Waltz; the least juicy was the variety Charlotte. The tastiest variety was the control variety Golden Delicious, followed by Mc. Intosh Wijcik and Waltz; the least tasty was the variety Bolero. In the overall sum of points appraising the fruit, fl esh and taste the variety Golden Delicious was at the top of the list, followed by Mc. Intosh Wijcik and Waltz. columnar apple trees, growth types, fruit-bearing, apple scab, powdery mildew
Screening of apple genotypes with the columnar growth habit using control markers
One of the approaches to intensify horticulture is to introduce cultivars with an unusual canopy into commercial production. Such plants can be columnar Apple trees. In modern breeding, there is a trend to create cultivars with a compact canopy. In such orchards, it is reduced to a minimum the cost for pruning, harvesting, and protection against pest and disease, which are the main expenses in apple orchards with a traditional canopy. The use of molecular markers linked to columnar growth habit allows us to identify a physiological sign at an early stage of growth: in the juvenile period. The assessment of apple cultivars and hybrids was carried out at the I. V. Michurin Federal Scientific Centre in 2015-2018. Four markers were used in the research: Mdo. chr 10.12, C18470-25831, 29f1, and jwlr to identify plants with the columnar growth habit gene (Co). The use of various DNA markers made it possible to establish that not all of them are well linked to the Co gene. In the research process, primers were identified for markers 29f1 and jwlr, which reliably allowed us to identify plants with columnar growth habit at the juvenile stage, which will significantly reduce the breeding process.
On the fractionation of β-lactoglobulin and α-lactalbumin
Abstract Existing methods for the preparation of α-lactalbumin and β-lactoglobulin are examined critically. It is proposed that the most satisfactory method for the preparation of the whey protein fraction from bovine milk is (NH 4 ) 2 SO 4 fractionation. A number of new gentle procedures for the fractionation of the whey proteins are compared with some existing methods. The distribution of total protein at various stages is determined by total Kjeldahl nitrogen and the kind of protein by starch-gel electrophoresis. The effect of variables, such as pH, salt concentration and temperature on the separation of α-lactalbumin and serum albumin from β-lactoglobulin is studied. Differences in the behavior ( e.g. solubility) of genetic variants of β-lactoglobulin are noted. The solubility of each of the major whey proteins, under similar conditions to those used in the fractionation, is compared with its solubility in the whey protein mixture. The understanding gained of factors important in the fractionation is used in recommending new procedures for α-lactalbumin and β-lactoglobulin isolation.