Progeny test of tetraploid Betula platyphylla and preliminary selection of hybrid parents
Estimating genetic parameters of parental lines through progeny testing and choosing good hybrid parents are important for genetically improving seed orchard trees. In this study, 24 tetraploid progeny seedlings were used as experimental materials, which came from test cross design: six tetraploid Betula platyphylla lines (Q33, Q13, Q103, Q19, Q83 and Q14) as female parents and four individual B. platyphylla diploid lines (F3, F4, F9 and F11) as male parents were crossed. Variance analysis of height, diameter, height-to-diameter ratio, and internodal distance showed that the differences between hybrid combinations reached highly significant levels. Using multi-objective decisionmaking, we performed a comprehensive assessment of the various hybrid combinations. Using a selection rate of 20 % of the standard, five hybrids were selected; their genetic gains in average height, diameter, height-to-diameter ratio, and internodal distance were 20.95, 6.07, 13.07 and 8.96 %, respectively. We also analyzed the combining ability and genetic parameter effect values of parents and hybrid combinations. The combined analysis revealed that Q13, Q103, Q33 and Q83 were superior females; F3, F4, and F9 were superior males; and F3 × Q13, F4 × Q83 and F9 × Q33 were superior hybrid combinations. The heights and diameters of these progenies were 22.49 and 11.48 % greater than average, respectively.
A comparison of the effect of genetic improvement, seed source and seedling seed orchard variables on progeny growth in Eucalyptus nitens in South Africa
Eucalyptus nitens is an important forestry species grown for pulp and paper production in the temperate, summer-rainfall regions of South Africa. A tree improvement programme has been ongoing at the Institute for Commercial Forestry Research for two decades, but genetic improvement in the species has been slow due to delayed and infrequent flowering and seed production. Three trials were established, firstly, to quantify the gains that have been made in the first generation of improvement in the breeding programme and, secondly, to establish whether a number of seed source and orchard variables influence the performance of the progeny. These variables were the amount of flowering trees in the seed orchard, year of seed collection, seed orchard origin and composition of seed orchard bulks. Diameter at breast height and tree heights were measured in the trials at between 87 and 97 months after establishment, and timber volumes and survival were calculated. Improved seed orchard bulks performed significantly better (p < 0.01) than unimproved controls in the field trials. Genetic gains ranging from 23.2 to 164.8 m3 ha−1 were observed over the unimproved commercial seed. There were significant differences (p < 0.01) in progeny growth between the levels of seed orchard flowering, with higher levels of flowering (≥40 %) producing substantially greater progeny growth than lower flowering levels (≤20 %). The seed orchard had no effect on progeny growth in this trial series. This suggests that seed collected from any of the four seed orchards tested will produce trees with significant improvement in growth.
Serum Profiles of Luteinizing Hormone, Oestradiol and Cholesterol and Ovarian Functions in Layer Poultry Birds (Gallus domesticus) Fed Diets Containing Furazolidone
This study was carried out to assess the serum profiles of luteinizing hormone (LH), oestradiol, cholesterol and ovarian functions in layer poultry birds (Rhode Island Red;Gallus domesticus) fed a diet containing various concentrations of furazolidone (FZ). A total of 40 birds were randomly assigned to receive FZ 0, 200, 400 or 800 mg/kg feed (ppm) daily during the pre-laying age, i.e. 13–18 weeks (for 5 weeks). Blood samples were collected at weekly intervals. Concentrations of LH and oestradiol in serum were estimated at alternate weeks using radioimmunoassays. Serum cholesterol levels were analysed by an enzymatic calorimetric method. Furazolidone administration was terminated at the 18th week of age. The birds were sacrificed at 22nd week of age and ovarian tissues were processed for morphometric studies. Serum LH, oestradiol and cholesterol levels were affected by age (p<0.001) and FZ dose (p<0.001). Serum LH and oestradiol levels were lower (p<0.05) in birds receiving FZ 800 mg/kg feed daily compared with the controls, whereas serum cholesterol profiles were lower (p<0.05) in all FZ-administered groups than in the control group. The mean weight of ovaries having no yolky follicles observed in the group receiving FZ 400 or 800 mg/kg feed per day was reduced (p<0.05) compared with the control group. Dosing FZ at 800 mg/kg feed per day reduced (p<0.05) the mean volume of ovaries having no yolky follicles compared with the control group. In birds receiving FZ 800 mg/kg feed per day, the mean length of the oviduct was reduced (p<0.05) as compared with the control group. Morphometric studies revealed that the mean number of oocytes with diameter in the range 401–800 μm decreased (p<0.05) in birds fed FZ 400 or 800 mg/kg feed per day. Initial egg production was affected by age (p<0.001) and dose (p<0.001) of FZ. The mean number of eggs laid by different groups revealed that egg production was reduced (p<0.05) in birds receiving FZ 800 mg/kg feed per day as compared with the controls. The present data suggest that FZ causes suppression in serum profiles of LH, oestradiol, cholesterol and ovarian functions in Rhode Island Red layer poultry birds. Therefore, great care must be taken with use of FZ in layer poultry birds (Gallus domesticus) with regard to dosage and duration of administration.