Biodegradation and Ecotoxicity of HFCs and HCFCs
Abstract. Hydrofluorocarbons (HFCs) and hydrochlorofluorocarbons (HCFCs) are used or developed as substitutes for fully halogenated chlorofluorocarbons. Based on the results of closed-bottle tests, the biodegradation of HFC-32, HCFC-123, HCFC-124, HFC-125, HFC-134a, HCFC-141b, HCFC-225ca, and HCFC-225cb was less than 60% after 28 days and therefore these compounds are considered not readily biodegradable. Standard acute toxicity tests with HCFC-123, HCFC-141b, and HCFC-225ca using algae, water fleas, and fish revealed EC50 values in the range of 17–126 mg/L. EC50 values of HFC-134a ranged between 450–980 mg/L. Fish studies with HCFC-141b and HCFC-225ca revealed bioaccumulation factors of <3 and 15–64, respectively. A study with plants revealed no effect of HCFC-141b on seed germination and growth of wheat (Triticum aestivum), radish (Raphanus sativus), and cress (Lepidium sativum). In conclusion, HFCs and HCFCs are not very toxic to aquatic organisms and terrestrial plants. No evidence for any aerobic biodegradation for most of the HFCs and HCFCs was found.
The role of CYP forms in the metabolism and metabolic activation of HCFCs and other halocarbons.
The use of hydrochlorofluorocarbons (HCFCs) such as HCFC-123 (2,2-dichloro-1,1,1-trifluoroethane) and HCFC-141b (1,1-dichloro-1-fluoroethane) is becoming widespread as replacements for the ozone depleting chlorofluorocarbons. Hepatic activation of HCFC-123 or the unsaturated perchloroethylene through oxidative pathways leads to the formation of the electrophiles trifluoroacetyl chloride or trichloroacetyl chloride, respectively. These can react with epsilon-NH(2) functions of lysine in proteins and give rise to neoantigens. In the case of HCFC-123, this reaction is catalysed primarily by CYP2E1 and to a much lesser extent by the constitutive CYP2C19, CYP2B6 and CYP2C8. For perchloroethylene, the extent of activation is less and the reaction is catalysed primarily by the CYP2B family. While acute hepatotoxicity has been seen in humans exposed to HCFC-123 or halothane, little short- or long-term toxicity in rodents is observed. No immunological related toxicity of perchloroethylene has been reported in exposed humans. Long-term exposure of rats can lead to renal tubule carcinomas and in mice, hepatocellular carcinomas. These toxic reactions do not appear to be directly related to the formation of the putative trichloroacetyl chloride intermediate.
SR topography of plastically deformed Fe-Si bicrystals - observation in situ and post mortem
SR reflection topography was applied to study the interaction of slip dislocations with the grain boundaries in Fe-4at%Si bicrystals deformed by compression. Three experimental settings with either a white or monochromatic beam were used and the corresponding orientation contrast analysed. Dislocation transmission through the grain boundary was observed in bicrystals with favourable geometry of the slip systems.