A Fast Optical Method for the Determination of Liquid Levels in Microplates
Parallel liquid handling systems are widely used in different applications of life sciences. In order to avoid false positive or negative results which lead to higher costs due to the replication of the experiments it is necessary to monitor the process and success of liquid delivery. An easy method for the determination of the liquid levels in microplates has been developed and evaluated. The optical method bases on the measurement of the liquid level using CCD cameras followed by special algorithms for the evaluation and visualization of the measured data. The proposed method was tested in changing environmental lighting for two different liquids. These tests confirm our approach towards optical liquid level determination for smallest volumes in microplates and also show the challenges regarding environmental lighting and different physical properties of fluids.
Camera grids for laboratory automation
This paper presents a straightforward method for mapping a multi-camera system to inspect multiple analytical samples carried in a microtiterplate, a labware standard used in life-science laboratories today. The high number of small samples that are provided by microplates burden difficulties when designing an inspection system with a single camera. The major concern is the perspective that is unique for every sample, and only a limited number of samples can be imaged in a favourable perspective, that is directly from below or above the sample. A way to overcome this problem is to use multiple cameras. This work presents the integration of an arbitrary number of cameras to support microplates of all types and densities. It provides a mapping framework to deliver the subimages to the inspection algorithms and includes a one-step registration procedure that uses a registration plate with microplate dimensions. Furthermore, an estimation of the system's mapping uncertainty is given.
Characterization of a tube-in-shell thermosyphon heat exchanger for solar water heating
Performance of a double-wall, natural convection, two-pass, tube-in-shell heat exchanger designed for use in a solar water heating system is measured. The heat exchanger is mounted along the length of a solar water storage tank. Flow of cold water on the shell side of the heat exchanger is driven solely by buoyancy forces. Hot side fluid is pumped. Flow rate, pressure drop and temperature data needed for a semi-empirical model of the heat exchanger are obtained. Performance is measured for forced convection, hot side volumetric flow rates of 0.016, 0.032 and 0.079 l/s. Temperature difference between hot and cold fluid at the inlets is varied from 0 to 75 degC. Measured values include thermosyphon and forced flow rates, temperature differences across the beat exchanger, vertical temperature distribution in the storage tank, and pressure drop due to shear on the thermosyphon side of the heat exchanger. Heat exchanger effectiveness and overall heat transfer area product (UA) are calculated. Thermosyphon mass flow rates are on the order of 0.01 kg/s. Although the low flow rates create thermal stratification of the water storage tank, they restrict energy transfer in the heat exchanger.