Cuevaenes A and B, new polyene carboxylic acids from Streptomyces sp. HKI 0180.
In the course of a screening for newmetabolites from strains of actinomycetes we disclosed Streptomyces sp. HKI 0180 as the producer of cuevaenes A (1) and B (2). The strain was isolated from a soil sample from the cave of Altamira (Spain). In this paper we report the production, isolation, structure elucidation and biological properties of 1 and 2 (Fig. 1). An agar slant culture of the above strain was used to inoculate a 500 ml Erlenmeyer flask containing 100ml of a seed medium composed of (g/liter): dextrose 15, soya bean meal 15, NaCl 5, CaCO3 1, KH2PO4 0.4, distilled water, pH 6.9. The producing medium contained (g/liter): mannitol 20, soya bean meal 20, distilled water, pH6.5.
New polyenic antibiotics active against gram-positive and gram-negative bacteria. IV. Structural elucidation of enacyloxin IIa.
The chemical structure of a unique polyenic antibiotic enacyloxin IIa (former name: fr. 2) produced by Frateuria (formerly Gluconobacter) sp. W-315 has been determined by extensive spectroscopic studies, in particular by NMR spectral analysis. It has a novel non-lactonic structure involving 3,4-dihydroxycyclohexanecarboxylic acid with a chlorine-containing polyenic and polyhydroxy acyl side chain attached as an ester to the 3-hydroxyl substituent of the acid.
New polyenic antibiotics active against gram-positive and -negative bacteria. I. Isolation and purification of antibiotics produced by Gluconobacter sp. W-315.
A new antibiotic, tentatively named as AB-315, was isolated from the fermentation broth of Gluconobacter sp. W-315. The antibiotic consists of a mixture of chemically related compounds. These compounds showed similar profiles in UV absorbancy. The antibiotics were active against Gram-positive and -negative bacteria, slightly active against fungi but not against yeasts.