Improved apparatus for trapped radical and other studies down to 1.5 K.
This note describes a Dewar system and associated apparatus for trapped free radical and other studies at temperatures down to 1.5 K. Cylindrical and rectangular microwave cavities can be used.
AN OPTICAL CELL FOR USE WITH LIQUID HELIUM
A low temperature cell is described that permits spectral absorption and emission measurements, as well as high energy irradiation of materials held at liquid helium temperatures. The liquid helium capacity of one liter is sufficient for up to 24 hours of continuous operation. Any substance whether it be solid, liquid, or gas at room temperatures can be studied. The all‐metal cell is entirely portable and can be employed with a number of instruments without modification. A sylphon bellows arrangement permits vertical displacement within the cell of the samples under study.
Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast.
Abstract The antibiotic cerulenin inhibited the incorporation of 14 C-acetyl-CoA by 67% at a concentration of 9 × 10 −6 M but not that of 14 C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of Saccharomyces cerevisiae . Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity.