Flow-injection analysis for meloxicam based on tris(2,2'-bipyridine) ruthenium(II)-Ce(IV) chemiluminescent system.
It was found that meloxicam could enhance the chemiluminescence (CL) of the tris(2,2'-bipyridine) ruthenium(II)-Ce(IV) system in the medium of sulfate acid. Based on this phenomenon a new flow-injection system with chemiluminescent detection has been proposed for determination of meloxicam. Under optimum conditions, meloxicam had a good linear relationship with the CL intensity in the concentration range of 6.0 10(-4) to 1.0 microg/mL and the detection limit was 3.7 x 10(-4 )microg/mL. The proposed method was applied to detect meloxicam in tablets and a satisfactory recovery was obtained. The possible mechanism for this CL system is also discussed in this paper.
Spectrophotometric Method for Determination of Meloxicam in Pharmaceutical Formulations Using N- bromosuccinimide as an Oxidant
A simple, rapid and sensitive spectrophotometric method has been developed for the determination of meloxicam (MX) in pure form and in its pharmaceutical preparations. The proposed method involve the addition of a measured excess of N-bromosuccinimide (NBS) in acid medium followed by determination of unreacted NBS by reacting with indigo carmine (IC) and measuring the absorbance at 610 nm. The optimum reaction conditions and other analytical parameters have been evaluated. Linearity was observed from 0.2-50 μg/ml meloxicam. Statistical analysis of the results and comparison with results by the British Pharmacopoeia method are also reported.
Improvement of scopolamine-induced memory impairment by Z-ajoene in the water maze in mice
Z-ajoene, a major compound containing sulfur in oil-macerated garlic products, exhibited inhibitory effects against scopolamine-induced memory impairment in mice using the Morris water maze test. The effects of Z-ajoene were observed dose-dependently (0.25-25 mg/kg). At the highest dosage, the memory performance of mice was improved compared to normal mice. The acetylcholinesterase (AChE) activity in the brain was reduced by administration of Z-ajoene dose-dependently. However, alliin and diallyl disulfide, organosulfur compounds from garlic, did not improve memory performance nor AChE inhibitory effect. These results suggest that Z-ajoene may act on the cholinergic system and on memory impairment caused by excess activity of AChE.