New inhibitors of melanogenesis, OH-3984 K1 and K2. II. Physico-chemical properties and structural elucidation.
New melanin synthesis inhibitors, OH-3984 K1 and K2, were isolated from the fermentation broth of Streptomyces sp. OH-3984, and their structures were elucidated by spectroscopic methods and by chemical transformations. OH-3984 K1 (M.W.: 306; C18H26O4) and K2 (M.W.: 308; C18H28O4) have unique gamma-lactone rings, both of which correspond to oxidative products derived from C1-O14 cleavage of the 14-membered lactone group.
Macrosphelide, a novel inhibitor of cell-cell adhesion molecule. II. Physiochemical properties and structural elucidation.
New anticell-adherence compounds, macrosphelides A and B, were isolated from the fermentation broth of Microsphaeropis sp. FO-5050, and their structures were elucidated by spectroscopic methods and chemical transformations. Macrosphelides A (M.W. 342, C16H22O8) and B (M.W. 340, C16H20O8) with three esters in their molecules were classified as 16-membered macrocyclic compounds. Macrosphelide B was found to be a corresponding oxidative product of macrosphelide A at the C-14 position.
Optical resolution of 2,6-diaminopimelic acid stereoisomers by high performance liquid chromatography for the chemotaxonomy of actinomycete strains
For the chemotaxonomy of actinomycetes, we describe the optical resolution method for 2, 6-diaminopimelic acid (DAP) stereoisomers by high performance liquid chromatography (HPLC) using 2, 3, 4, 6-tetra-O-acetyl- β-D-glucopyranosyl isothiocyanate (GITC). The analysis was applicable both to whole-cell preparations and to cell wall ones. This method made it possible to analyze quantitatively LL-, DD-, and meso-DAP, compared with the previously reported methods of paper chromatography, thin-layer chromatography and HPLC.