Culture conditions for efficient induction of green plants from isolated microspores of barley
Subculture regime and carbohydrate concentration of the medium had a marked effect on the regeneration of green plantlets from mechanically isolated microspores of Hordeum vulgare L. cv. Kymppi. A sevenfold increase in the yield of green plants was obtained by shortening the suspension culture time of the developing proembryo mass from 4 to 3 weeks. A further twofold increase was obtained by increasing the maltose concentration of the microspore isolation medium and of the culture medium. Under optimal conditions, a mean of 169±97 green plants per spike were regenerated.
Microspore culture of Hordeum vulgare L.: the influence of density and osmolality
SummaryDonor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum plating density is achieved by adjusting the density to 2×104 embryogenic microspores per ml, with a lower threshold at 5×103 per ml. By increasing the osmolality of the pretreatment solution to 440 mOs.kg−1 and that of the culture medium to 350 mOs.kg−1, up to 15% of the population developed into embryo-like structures. When microspores of cv. Igri were cultured under the optimized conditions, the ratio of green/albino plants increased from 1∶1 to 34∶1, and 50 green plants per anther were formed.
Researches on Structural Factor Involvement in Oral Tribology
This study is focused on two ceramic materials, D.SING (Ivoclar) and VM13 (emphasis the VITA TN), frequently used in restorative dentistry practice and it comes to complete a full set of results obtained by the authors over several years of research on oral tribology with main influence on oral health. In this way there are involved more specific structural aspects of dental restorative ceramic materials quality as is shown in [1].