Life histories and host specificities of theEchium flea beetlesLongitarsus echii andL. Aeneus [Col. Chrysomelidae]
Two halticine flea beetles,Longitarsus echiiKoch andLongitarsus aeneusKutsch, occurring on the boraginaceous plantEchium plantagineum L. in the western Mediterranean region are potential biological control agents in Australia where the plant has become a weed (Paterson's curse). The adults of both species feed by making “shot holes” in the leaves. The larvae of both species attack the roots ofEchium, those ofL. echii feeding more centrally in the root than those ofL. aeneus which attack the outer cortex and rootlets. Both species are univoltine. The host restrictions of both flea beetles as adults and as larvae were tested using the criteria already established for theEchium leaf minerDialectica scalariellaZeller (Wapshere & Kirk, 1977). This testing established that it would be safe to introduce the 2 insects into Australia for the biological control ofE. plantagineum and they are now under quarantine.RésuméDeux altises,Longitarsus echiiKoch etLongitarsus aeneusKutsch, vivant surEchium plantagineum L. dans l'ouest de la région méditerranéenne, sont des agents de contrôles biologiques potentiels de cette adventice en Australie. Les adultes des 2 espèces creusent des trous dans les feuilles et leurs larves attaquent les racines de la plante. Les larves deL. echii se nourrissent plus de la partie centrale de la racine, alors que celles deL. aeneus préfèrent le cortex extérieur et les radicelles. Les 2 espèces ont une génération par an. La spécificité d'hôte des 2 espèces, tant adultes que larves, a été étudiée selon les critères déjà établis pourDialectica scalariellaZeller (Wapshere & Kirk, 1977). Ces essais ont confirmé que l'introduction des 2 insectes en Australie pour la lutte biologique contreEchium plantagineum serait sans danger, et cette introduction a eu lieu récemment.
The host range and impact of Bikasha collaris (Coleoptera: Chrysomelidae), a promising candidate agent for biological control of Chinese tallow, Triadica sebifera (Euphorbiaceae) in the United States
Native to China, the Chinese tallow, Triadica sebifera (Euphorbiaceae) is an aggressive woody invader in the southeastern United States. The flea beetle, Bikasha collaris (Coleoptera: Chrysomelidae), is a common herbivore attacking this plant in China. To evaluate its potential as a biological control agent the host specificity and impact were studied in China. The potential host range of B. collaris was evaluated on 68 plant species in 20 families through no-choice and choice tests. Adults only survived and fed on plants from the genus Triadica while larvae only developed successfully on T. sebifera in no-choice tests. Under choice conditions, adults showed a distinct preference for T. sebifera over other Triadica species. The impact of the above- and belowground herbivory on the growth of T. sebifera was evaluated in a common garden.B. collaris could reduce stem height, total biomass, number of leaves and stem diameter at a density of 10 individuals per plant. Above- and belowground herbivory had similar effects on plant growth parameters. The results of this study suggested that B. collaris is a potential biological control agent of T. sebifera though further tests on native North American species are needed.
Synergistic Effect of High Temperature and High pH on the Destruction of Salmonella enteritidis and Escherichia co1i O157:H7.
This study was undertaken to determine if high temperature and high pH interact synergistically to enhance the rate of destruction of two important gram-negative foodborne pathogens, Escherichia coli O157:H7 and Salmonella enteritidis . The rates of destruction in NaHCO3-NaOH buffers at pH 7.0, 10.0, and 11.0 were determined at 35, 40, 45, 50, 55, 60, and 65°C. Use of an improved heating protocol eliminated a "tailing effect" at longer exposure times. The present study demonstrated that the combination of high pH and high temperature resulted in a highly significant synergistic interaction (P > F = 0.0001), which caused rapid death of both E. coli O157:H7 and S. enteritidis . This "alka-therm" technology might be used commercially to destroy gram-negative foodborne pathogens on various raw agricultural commodities.