Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography
A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C18 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.
Rapid determination of paeoniflorin
Sir, I read the recent publication on rapid determination of paeoniflorin with great interest.[1] Zhou et al. concluded that their new proposed method is a rapid, effective, binary reverse phase rapid resolution liquid chromatographic (LC) method.[1] I have some comments on this work. First, I have a concern on the cost of this new LC method. Is it expensive that prevents it from application in routine usage? Second, the quality management, especially for external quality assessment, is a problem that might prevent the generalization of this new technique.[2] Third, the LC is not the fastest technique for identification. Fluorescenceand electrochemical-based detections might be faster.
Structure of gallotannins in Paeoniae Radix.
The gallotannins (polygalloylglucoses) were isolated homogeneously from Paeoniae Radix and their structures were elucidated by 13C-nuclear magnetic resonance spectroscopy.