High-frequency-noise-induced hearing loss: a field study on the role of intensity level and accumulated noise dose
SummaryBased on audiometric tests in the range of 10 to 20 kHz, of 106 ultrasound operators, as well as on measurements of high-frequency noise, the problem of safety limits for high-frequency noise exposure was investigated. Analyzing the relation between noise levels of 1/3 octave bands at center frequencies of 10, 12.5 and 16 kHz and the accumulated noise dose on the one hand, and changes of hearing at 10 to 12, 11 to 13 and 14 to 16 kHz respectively, on the other hand, a harmless level up to 80 dB and a harmless noise dose up to 1 unit for people not older than 40 years have been found. For older people this level and this noise dose can be dangerous.
High-frequency hearing thresholds: effects of age, occupational ultrasound and noise exposure
AbstractPurpose
It has been suggested that high-frequency audiometry (HFA) could represent a useful preventive measure in exposed workers. The aim was to investigate the effects of age, ultrasound and noise on high-frequency hearing thresholds.MethodsWe tested 24 industrial ultrasound-exposed subjects, 113 industrial noise-exposed subjects and 148 non-exposed subjects. Each subject was tested with both conventional-frequency (0.125–8 kHz) and high-frequency (9–18 kHz) audiometry.ResultsThe hearing threshold at high frequency deteriorated as a function of age, especially in subjects more than 30 years old. The ultrasound-exposed subjects had significantly higher hearing thresholds than the non-exposed ones at the high frequencies, being greatest from 10 to 14 kHz. This hearing loss was already significantly evident in subjects with exposure <5 years and increased with years of exposure and advancing age. The noise exposure group had significantly higher hearing thresholds than the non-exposed group at the conventional frequencies 4 and 6 kHz and at the high frequency of 14 kHz. After stratification for age, there was a significant difference between the two groups at 9–10 and 14–15 kHz only for those under 30 years of age.ConclusionMultivariate analysis indicated that age was the primary predictor, and noise and ultrasound exposure the secondary predictors of hearing thresholds in the high-frequency range. The results suggest that HFA could be useful in the early diagnosis of noise-induced hearing loss in younger groups of workers (under 30 years of age).

The peculiar thermo-structural behavior of the anionic lipid DMPG.
Aqueous dispersions of the anionic phospholipid dimyristoyl phosphatidylglycerol (DMPG), around 100 mM ionic strength, are known to exhibit a thermal behavior similar to that of the largely studied lipid dimyristoyl phosphatidylcholine (DMPC), which undergoes a gel to liquid crystalline phase transition at 23 degrees C, well characterized by differential scanning calorimetry (DSC), and other methods. However, at low ionic strength, DMPG has been shown to present a large gel-fluid transition region, ranging from 18 to 35 degrees C. This intermediate phase is optically transparent and characterized by a continuous change in membrane packing. Structural properties of the DMPG gel-fluid transition region will be discussed, based on results obtained by several techniques: electron spin resonance (ESR) of spin labels at the membrane surface and intercalated at different depths in the bilayer; light scattering; DSC; small angle X-ray scattering (SAXS); and fluorescence spectroscopy of probes in the bilayer.