Dry mass of Fusarium roseum spores before and after germination
The total dry mass of Fusarium roseum spores and contained lipid bodies were determined before and after spores germinated using quantitative interference microscopy. The mean for spore dry mass before germination was about 57 pg. Lipid bodies accounted for about 61% of that. Areas of lipid bodies in spores before and after germination were about 23 % but the contents of the lipid bodies accounted for only 10% of the spore dry mass after germination. The total dry mass of the spore and germ tube(s) greatly exceeded that of the spore before germination. We infer that nutrients for germ tube growth are derived from within the germinating spore and from the medium which must contain nutrients leached from non-germinating spores.
Dry mass changes in germinating spores of Diplodia maydis
SummaryThe dry mass of two-celled Diplodia maydis spores was measured both before and after germination by quantitative interference microscopy. The dry mass of spores declined approximately 50% during germination. However, the dry mass of germinating spores plus the dry mass of their germ tubes was greater than the dry mass of spores before germination. We conclude that the germinating spores absorbed nutrients released from non-germinating spores.The dry mass of fungal spores can be estimated by weighing large numbers of spores and determining the mean from sample spore counts. Mumford and Pappelis(4) determined the total dry mass of individual spores of Fusarium roseum and the contained lipid bodies before and after spores germinated using quantitative interference microscopy. The mean spore dry mass before germination was 57 pg. Lipid bodies accounted for about 61% of that mass and decreased as spores germinated. The total dry mass of the spore and germ tube 24 hr later greatly exceeded that of the spore before germination. Quantitative interference microscopy has been used to measure the dry mass of various types of cells. Kulfinski and Pappelis (3) recently reviewed how this technique has been applied to plant cells. Technical aspects of interference microscopy have been described by Ross (6).The purpose of this study was to examine the dry mass changes in Diplodia maydis (Berk.) Sacc. with and without germ tubes through the use of interference microscopy.
Numerical and experimental investigation on air distributor design of fluidized bed reactor of sawdust pyrolysis
Abstract An improved biomass conversion in a lab-scale fluidized bed reactor (FBR) is achieved by optimizing the air distributor orifice size and number. Two approaches for introducing air were numerically and experimentally examined. These included perforated plate and downward orifice sparger. 3 mm and 5 mm orifice diameters, and 7, 9 and 13 holes were simulated at flow velocities of 2, 4, 6, and 8 times the minimum fluidization velocity, Umf. The simulation results revealed that increasing the orifice number has a positive effect on gas holdup, particularly for the perforated plate, which showed a uniform distribution of bubbles. The sparger configuration produced larger bubbles sizes, which could lead to better recirculation of solid flux, while stagnant particle zones were observed at the lower section of both distributors. A pyrolysis unit is also inserted at the top of freeboard of the FBR. For perforated plate with 6Umf, the pyrolysis process achieved thermal and cold gas efficiencies of 82.91% and 52.03%, respectively, while combustible gases with 41.6%mol CO, 8.7%mol CH 4 and 2.17%mol HC, were obtained. For sparger pipe and 6U mf _sp, 35.33 ppm NOx and 111 ppm SO 2 were measured in flue gas from combustion process at bed region.