ISOLATION AND CHARACTERIZATION OF MYCOPLASMA AND ACHOLEPLASMA FROM PNEUMONIC LUNGS OF GOATS SLAUGHTERED AT FAISALABAD ABATTOIR 0
Among the goats slaughtered at Faisalabad abattoir, pneumonic lungs were collected from 50 animals suspected for contagious caprine pleuropneumonia (CCPP). These samples were subjected to bacteriological studies and 8 isolates were recovered. On the basis of morphological, biochemical a 1d serological characteristics, these were identified as Mycoplasma mycoides subspecies capri (4), acholeplasmas (2) and unidentified mycoplasmas (2).
Caprine pneumonia. II. Biochemical characterisation and serological identification of mycoplasmas.
Thirty strains Mycoplasma isolated from pneumonic lungs of goats were chracterised biochemically and serologically. On the basis of biochemical characteristics, the strains were divided into three groups. (A) Eighteen strains which fermented glucose and mannose, digested serum and reduced tetrazolium, (B) 10 strains which reduced tetrazolium, formed "film and spots" and possessed phosphatase activity and (C) two strains which catabolised arginine only. Serologically group A strains were identified as M. mycoides subspecies, group B strains could not be fully identified but on the basis of immunofluorescence and biochemical reactions were put in the M. agalactiae subspecies; there are to be further investigated. Group C strains were identified as M. arginini.
Diagnostic and therapeutic studies on chronic diarrhea in dairy animals
The study was conducted to determine the etiology of chronic diarrhea in dairy animals. Seven causes of chronic diarrhea were established in dairy cattle and buffaloes. Balantidiasis in 37.6 per cent, dietary diarrhea in 9.9 per cent, chronic salmonellosis in 6.9 per cent cases of chronic diarrhea, secondary copper deficiency and amphistomiasis in 6.9 per cent, clinical Johne‘s disease in 3.9 per cent and chronic peritonitis in 2.97 per cent of dairy animals, manifest chronic diarrhea. The precise diagnosis could not be established in 24.50 per cent of animals. Lymphosarcoma was confirmed in one of the dairy cattle. Body condition score was associated to the etiology of chronic diarrhea. B. coli infected cattle and buffalo revealed cysts in 32.3 per cent cases while 5 per cent positive cases revealed both cysts and trophozoites. Mean cyst count was 2.73±0.91 hpf in diarrheic animals compared to 0.11±0.447 hpf in healthy animals. Clinical Johne‘s disease was confirmed in four animals. Hematological changes revealed anemia and neutrophilia with morphological changes suggestive of regenerative to degenerative left shift. High plasma phosphorus, hypoproteinaemia and hypoalbuminaemia was seen in clinical Johne‘s disease. Salmonella isolates showed higher in-vitro sensitivity to enrofloxacin (87%), ciprofloxacin (87%), gentamicin (75%), and tetracycline (66.7 %) and were resistant to ampicillin and amoxicillin. Mean cyst count reduction from 2.41±1.16 to 0.11±.33 was observed in oral metronidazole treated group where as reduction of mean cyst count from 2.73±0.918 to 0.18±0.44 in intravenous metronidazole treated group. Recovery rate with oral and intravenous metronidazole was 42.1 and 63.1 per cent respectively. Recovery results in significant decrease in eiosionphil count and increase in total plasma protein in both oral and intravenous treatment groups. Complete recovery response approaches 60 per cent in balantidiasis affected adult dairy animals. Balantidiasis was the major cause of chronic diarrhea and intravenous administration of metronidazole was more effective than oral metronidazole.