Study on preparing fatty acid by hydrolyzing Jatropha Curcasl Oil with lipase
Used tween-80 as emulsifier, industrial lipase as catalyst to hydrolyze Jatropha Curcasl Oil and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants as follows: concentration of catalyst 4% (W/W), weight ratio of oil to water 1:4 (W/W), temperature at 40°C, and hydrolysis time is 32h. Under these conditions, the AV(acid value) of hydrolyzed rapeseed oil reached 163.2 mg KOH/g, and hydrolytic rate arrived at 89.6%.
Study on preparing fatty acid by hydrolyzing rapeseed oil with lipase
Used Tween-80 as emulsifier, industrial lipase as catalyst to hydrolyze rapeseed oil and make mixed fatty acid. We discussed the enzyme concentration, substrate concentration, temperature and time on hydrolysis. Through the experiment, we found that when we used emulsifier which contains 2% oil in the system, we could get the optimal parameter for hydrolysis of waste oil from restaurants as follows: concentration of catalyst 5% (W/W), weight ratio of oil to water 1∶5 (W/W) , temperature at 40°C, and hydrolysis time is 32h. Under these conditions, the acid value of hydrolyzed rapeseed oil reached 175.8 mg KOH/g, and hydrolytic rate arrived at 91.5%.
Biochemical modification of fats by microorganisms: A preliminary survey
Over 100 different strains of bacteria, actinomycetes, fungi and yeasts were incubated at 28 C for five days in the presence of soybean oil. Some soybean oil was consumed by many microorganisms, and some was also hydrolyzed to free fatty acids.Aspergillus oryzae, two different strains ofAmylomyces rouxii andRhizopus oligosporus hydrolyzed the oil completely (95%). The fatty acids fromAspergillus flavus fermentation contained less linolenic acid than the original soybean oil. Lipase was found intra- and extracellularly when microorganisms were grown in the presence of soybean oil.