5 p-CHOLESTANE · 3 a , 27-DIOL : NAD + OXIDOREDUCTASE AND 3 a-HYDROXY-5 p-CHOLESTAN-27-AL : NAD + OXIDOREDUCT ASE IN RAT LIVER EXTRACT * )
Using rat liver alcohol:NAD+ oxidoreductase and aldehyde:NAD+ oxidoreductase, the following facts were found: 1) 3a-hydroxy-5~-cholestan-27-al was reduced to 5fi -cholestane-3a, 27-diol by liver alcohol: NAD+ oxidoreductase. 2) The optimum pH was pH 7. 0 with potassium phosphate buffer and pH 8. 5 with Tris buffer. 3) The Km value of the substrate to the enzyme was 14. 3 μM. 4) 3a-Hydroxy5fi-cholestan-27al was oxidized to 3a-hydroxy5fi-cholestan27-oic acid by liver aldehyde: NAD+ oxidoreductase. 5) The optimum pH was pH 8. 5 with Tris buffer. 6) The Km value of the substrate to the enzyme was 26. 3 μM.
Horse liver 3 ,7 ,12 -trihydroxy-5 -cholestan-26-al dehydrogenase as a liver aldehyde dehydrogenase.
Abstract 3α,7α,12α-Trihydroxy-5β-cholestan-26-al dehydrogenase (3α,7α,12α-trihydroxy-5β-cholestan-26-al + NAD+ + H2O → 3α,7α,12α-trihydroxy-5β-cholestan-26-oic acid + NADH + H+) in a soluble fraction of horse liver was studied. 1. 1. Horse liver 3α,7α,12α-trihydroxy-5β-cholestan-26-al dehydrogenase consists of two components, the major one of which was purified about 70-fold by (NH4)2SO4 fractionation, gel filtration, ion-exchange column chromatography and hydroxylapatite column chromatography. 2. 2. 3α,7α,12α-trihydroxy-5β-cholestan-26-al dehydrogenase was always accompanied by liver aldehyde:NAD oxidoreductase (EC 1.2.1.3) activity, and the ratio of these two enzyme activities was not altered by purification. 3. 3. Both 3α,7α,12α-trihydroxy-5β-cholestan-26-al dehydrogenase and horse liver aldehyde:NAD+ oxidoreductase were reduced to a similar extent on inactivation of the purified enzyme by heating or by incubation with p-chloromercuribenzoate. 4. 4. The Km value for NAD+ of 3α,7α,12α-trihydroxy-5β-cholestan-26-al dehydrogenase was found to be roughly equal to that of liver aldehyde:NAD+ oxidoreductase. 5. 5. The possibility was discussed that both 3α,7α,12α-trihydroxy-5β-cholestan-26-al dehydrogenase and liver aldehyde:NAD+ oxidoreductase activities may be due to a single protein.
An introduction to dramatherapy
What Is Dramatherapy? Theory into Practice Preparing for Dramatherapy Beginning a Dramatherapy Session Applying Dramatherapy The Heart of Dramatherapy Dramatherapy and 'Mental Illness' Dramatherapy and Other Forms of Disorder of Mind Other Client Groups and Dramatherapy