Alkaloids ofThalictrum collinum
The method of methanolic extraction was applied to the roots. After the methanol has been distilled off, the concentrated extract was treated with 10% sulfuric acid, the acid extracts were alkalinized to pH 9, and the alkaloids were extracted with ether and chloroform. The combined ether-soluble and chloroform-soluble alkaloids obtained were separated into phenolic and nonphenolic bases. The combined nonphenolic tertiary bases were chromat.graphed on a column of alumina (1:70, neutral, activity grade II). The alkaloids were eluted with benzene, benzene--chloroform, chloroform, ethyl acetate, and ethyl acetate-methanol.
New Flavonoid Glycoside from Thalictrum minus
The new flavonoid diglycoside thamiflaside was isolated from the aerial part of Thalictrum minus. The chemical structure of this glycoside was determined as apigenin 7-O-α-L-2″′-methoxyrhamnopyranosyl-(1→6)-β-D-glucopyranoside based on PMR and 13C NMR (DEPT, HSQC, HMBC) physicochemical methods.
THE IDENTIFICATION AND DETERMINATION OF NITROGENOUS ORGANIC BASES WITH AMMONIUM REINECKATE
The identification and determination of several important bases as their reineckates is described. The ultra‐violet and visible spectra of reineckates were studied and used for the determination of molecular weights and solubilities and for the determination of bases. This paper also records as yet unreported constants of the characteristic mono‐ and di‐reineckate derivatives of several clinically important compounds. A method for the regeneration of the conjugate bases from the reineckates using ion exchange resins is also given.