The genetic variability of the yield components in the Kenyan pyrethrum population
SummaryPyrethrum is grown for the insecticidal ‘pyrethrins’, extracted from the dried and ground flowers. It is a cross-fertilizing, self-incompatible perennial plant, which can be propagated vegetatively.The genetic variation of several, commercially important, characteristics was studied. The range of observed variation is very wide for fresh flower yield, flower size, number of flowers per stem, pyrethrins content, lodging resistance and the Pyrethrins I/Pyrethrins II ratio, and quite narrow for the dry matter content of the flowers. Populations to select from are obtained by crossing two (single crosses) or more clones (polycrosses). The genotypic variation of two single and one polycross (42 clones) was studied. The single crosses showed, for the three characteristics measured, pyrethrins content, flower size and Py. I/Py. II ratio, a considerable transgression. The progeny means were very similar to the mid-parent values. This indicates a polygenic inheritance for these traits. The variation of the polycross for these three traits was not greater than those of the two single crosses, suggesting a very high level of heterozygosity in the parental clones. It is in fact possible to reconstitute a large part of the genotypic variation observed in Kenya from only a very few single crosses. In view of the selection relationships between traits were investigated. Flower size appeared negatively related to dry matter content of the flowers and to flower yield. A suspected negative relation between flower yield and pyrethrins content could not be confirmed. The results indicate, that large genetic improvements are possible in a fairly short time.
Chilling and Flower-bud Stimulation in Pyrethrum (Chrysanthemum cinerariaefolium)
The flowering of pyrethrum at the normal altitudes at which it is cultivated in East Africa depends on the amount of chilling available for the earlier stimulation of the flower-buds. The period of maximum flower production follows some 3 months after the period of maximum cold in the cold season. Attempts were made to delay the period of maximum flower production by cutting back new growth. These failed because chilling was so intimately connected with flower-bud development. A tentative upper limit of temperature for flower production in East Africa varieties has been selected.
Staining and observing pollen tubes in the style by means of fluorescence.
Pollen tubes in the styles of the tomato and of other flowering plants can be observed by using the following technic. Styles are fixed in formalin-acetic-80% alcohol (1:1:8) and cleared and softened in a strong (8 N) sodium hydroxide solution. Staining is accomplished in a 0.1% solution of water-soluble aniline blue dye dissolved in 0.1 N, K3PO4. The styles are smeared or are observed whole under a conventional or dissecting microscope by direct illumination with ultraviolet light of a wavelength of about 356 m°. Observations are made in a darkened room. Under these conditions callose fluoresces bright yellow-green and contrasts strongly with the bluish or grayish fluorescence of the stylar tissue. The pollen tubes are outlined by a callose lining and irregularly spaced callose plugs.