In Vitro Propagation of Selected Sugarcane ( Saccharum Officinarum L . ) Varieties ( C 86-165 and C 86-12 ) Through Shoot Apical Meristem
Sugarcane (Saccharum officinarum L.) is monocotyledonous crop plant that belongs to the Poaceae family that mostly propagates through conventional methods. However, conventional propagation lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize in vitro micro propagation protocol for two sugarcane varieties (C 86-165 and C 86-12) using shoot apical meristem explants. The two varieties were cultured on MS medium supplemented with different concentrations of growth regulators on shoot initiation, multiplication and root induction stages. Analysis of variance (ANOVA) revealed that the two varieties showed statistically significant difference in their response to the various hormonal treatments with regard to the parameters measured. For initiation stage vars. C 86-165 and C 86-12 performed best on 1.0 mg/l and 0.5 mg/l of BAP, respectively. On the other hand, multiplication stage was best in MS media enriched with 2.0 mg/l BAP + 0.5 mg/l NAA and 1.5 mg/l BAP + 0.5 NAA as manifested in terms of number of shoots and shoot length per explants after four weeks of culture for vars. C 86-165 and C 86-12, respectively. With regard to root induction, best rooting response in terms of mean root number, mean root length, mean fresh weight and mean dry weight was achieved in 1/2 MS media enriched with 2.0.mg/l NAA and 1.0 mg/l NAA after four weeks of culture for vars. C 86-165 and C 86-12, respectively.
Comparative Performance of Micropropagated and Conventionally Raised Crops of Sugarcane
A comparative study on the performance of tissue culture raised and conventional crops of sugarcane was made at eight locations in sugar mill area of Nahar Sugar and Allied Industries Ltd., Amloh (Punjab). In vitro raised plantlets of sugarcane varieties viz. CoJ 64 and CoJ 83 were hardened, acclimatized and transplanted to the fields for cane formation. After maturity, the seed material was again sown in the field by sett cutting method along with appropriate control for field evaluation of the crops. On the basis of data, it was observed that the tissue culture raised crop was superior in both agro-morphological and quality traits over the crop raised by conventional method.
How Does the Keyword Method Affect Vocabulary Comprehension and Usage? Report from the project on Studies in Language: Reading and Communication. Working Paper No. 278.
THE KEYWORD METHOD of vocabulary learning involves forming a linkage between a to-be-learned vocabulary word and a familiar English word that sounds like part of the to-be-learned item (the keyword). Then the learner forms an interactive image between the keyword and definition referents. In previous research, the keyword method has been found to facilitate students' recall of definitions, given vocabulary items. In this study, use of the method was also shown to increase comprehension of English vocabulary in context, as well as usage of that vocabulary in a novel context.