NEAR INFRA RED SPECTROSCOPIC ANALYSIS OF MACROTYLOMA UNIFLORUM ( LAM . ) VERDC . , PHASEOLUS LUNATUS LINN . AND PHASEOLUS VULGARIS
The present study is designed to determine the types of secondary metabolites in three selected seed powders by fast, reliable, and non-destructive Near Infra Red spectroscopic analytical technique. NIRS analysis of Macrotyloma uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., and Phaseolus vulgaris Linn., seed flours were done for qualitative evaluation of secondary metabolites. The fine powder of crude drug was directly applied in FOSS XDS near infrared Rapid Content Analyzer. The properly filled powdered sample in a disposable glass silicate vial and closed with plastic lid was centrally placed onto the sample presentation glass of Foss Rapid Content Analyzer. The scanning of sample at ambient temperature gives NIR spectra within 30 seconds. The major proposed compounds in M. uniflorum were 3,4-dihydroxybenzoic acid, caffeic acid, chlorogenic acid, ferulic acid, gallic acid, kaempferol, linolenic acid, niacin, quercetin, sinapinic acid, syringic acid, and vanillic acid. Whereas, coumaric acid, fructose, galactose, L-serine, raffinose, stachyose, sucrose, threonine, and tyrosine were detected in P. lunatus and the NIR suggested the presence of caffeic acid, ferulic acid, sinapinic acid, phytic acid, leucine, and valine in P. vulgaris. NIRS as an advance innovative technology extensively used in chemical, food, petrochemical, and pharmaceutical industries for rapid and reliable standardization. The present study revealed a good correlation to conclude that flour samples of all the three legume seeds could potentially be added to food system to achieve nutritional and functional benefits.
Fatty Acid, Flavonol, and Mineral Composition Variability among Seven Macrotyloma uniflorum (Lam.) Verdc. Accessions
Horse gram [ Macrotyloma uniflorum (Lam.) Verdc.] seeds containing high concentrations of fatty acids, flavonols and minerals should provide government, public and private organizations with a nutritious and healthy food for use by malnourished and food deprived people worldwide. Seeds from seven horse gram accessions, geographically adapted to Griffin, GA, USA were analyzed for fatty acid, flavonol, and mineral concentrations using gas chromatography, reverse-phase high performance liquid chromatography, and inductively coupled plasma-optical emission spectroscopy, respectively. Significant year effects occurred for stearic, oleic, linoleic, arachidic, gadoleic, and lignoceric acids. Oleic, linoleic, and linolenic acid ranged from 8.9%–16.8%, 40.3%–45.6%, and 11.6%–14.3%, respectively, as percent of total fatty acids measured (total oil ranged from 2.32% to 2.87%). Seed concentrations of myricetin, quercetin, and kaempferol ranged from 0–36 μg/g DW, 0–27 μg/g DW, and 240–316 μg/g DW, respectively and the only year effect was observed for kaempferol among the horse gram accessions. Year effects were found for Fe, K, Mg, Mn, Ni, and S. Mean concentrations of macrominerals (Ca, K, Mg, P, and S) and microminerals (Cu, Fe, Mn, Ni, and Zn) ranged from 1.3–14 mg/g DW, and 1.0–95.0 μg/g DW, respectively. Several correlations were observed among several fatty acids, flavonols, and minerals. The mono-unsaturated fatty acid, oleic acid correlated significantly with linoleic acid ( r = −0.64), arachidic acid ( r = −0.61), Ca ( r = 0.50) and Zn ( r = 0.51, all at P < 0.01). The flavonol, myricetin correlated significantly with quercetin ( r = 0.92, P < 0.0001), while quercetin correlated with Ca ( r = 0.82, P < 0.0001) and kaempferol correlated with Mg ( r = 0.61, P < 0.01). Several mineral correlations were found including Fe with K ( r = 0.66) and Mg ( r = 0.56, both at P < 0.01). These seven horse gram accessions can be used in breeding programs to facilitate the production of superior cultivars with favorable fatty acid profiles, flavonol content, and mineral compositions.
Synergistic Effect of High Temperature and High pH on the Destruction of Salmonella enteritidis and Escherichia co1i O157:H7.
This study was undertaken to determine if high temperature and high pH interact synergistically to enhance the rate of destruction of two important gram-negative foodborne pathogens, Escherichia coli O157:H7 and Salmonella enteritidis . The rates of destruction in NaHCO3-NaOH buffers at pH 7.0, 10.0, and 11.0 were determined at 35, 40, 45, 50, 55, 60, and 65°C. Use of an improved heating protocol eliminated a "tailing effect" at longer exposure times. The present study demonstrated that the combination of high pH and high temperature resulted in a highly significant synergistic interaction (P > F = 0.0001), which caused rapid death of both E. coli O157:H7 and S. enteritidis . This "alka-therm" technology might be used commercially to destroy gram-negative foodborne pathogens on various raw agricultural commodities.