Variation for winter hardiness generated by androgenesis from
Festuca pratensis × Lolium multiflorum amphidiploid cultivars with different winter susceptibility
Androgenic populations produced from three Festuca pratensis × Lolium multiflorum amphidiploid (2n = 4x = 28) cultivars show wide within population variation for winter hardiness. Populations comprising a total of 423 androgenic plants derived from 23 donor plants from 3 Polish F. pratensis × L. multiflorum cultivars Felopa, Sulino, and Rakopan were studied over 3 years (1999–2002) in a field experiment at Lopuszna, Poland (20∘08′E, 49∘28′N, altitude 568 m). The results indicate that despite differences in winter hardiness between the three amphidiploid cultivars, the mean winter hardiness of androgenic progeny from each cultivar was the same. Thus an androgenesis component in a plant breeding programme may provide an opportunity to recover winter hardy genotypes from high yielding cultivars that are themselves adapted poorly to stress conditions. Androgenesis also evoked variation in snow mould resistance. In this case, androgenic plants with the greatest snow mould resistance were recovered from the least winter hardy donor cultivars and plants. The results indicated the low importance of snow mould resistance as a component of winter hardiness under the field conditions used for these experiments.
Androgenesis from a Lolium multiflorum × Festuca arundinacea hybrid to generate extreme variation for freezing-tolerance
New opportunities for plant breeding using androgenesis in Lolium × Festuca hybrids have been identified. Plants derived by anther culture from a Lolium multiflorum × Festuca arundinacea (5x) hybrid were screened for freezing-tolerance, and their post-freezing recovery compared. The androgenic population showed extreme diversity in freezing-tolerance. While the majority of androgenic plants had inferior freezing-tolerance compared with the freezing-sensitive L. multiflorum parent, 6% of the population were more freezing resistant than the freezing-tolerant Festuca parent. Novel Lolium and Festuca gene combinations resulting from rare meiotic events were recovered within the androgenic population. The two most freezing-tolerant androgenic plants carried virtually the entire F. pratensis subgenome of F. arundinacea. F. pratensis is known to carry genes for freezing-tolerance and would be expected to be the primary source of genes governing this trait within the F. arundinacea genome. The most freezing-tolerant androgenic plants were more freezing-tolerant than the hybrid plant from which they were derived. Consequently, androgenesis was also effective in removing factor(s) reducing the expression of freezing-tolerance within the L. multiflorum × F. arundinacea (5x) hybrid.
Avocado Flowering 1
The avocado (Persea americana Mill.) has increased in importance over the years in many countries around the world. The United States, Mexico and Brazil lead in world commercial production, while Israel, South Africa, Australia, New Zealand, the Philippines, Spain, Cyprus, Canary Islands, Chile, Ecuador, Venezuela, Greece, and the Caribbean area are actively increasing commercial production (B.O. Bergh, personal communication; Gustafson 1976). The history of the avocado has been detailed by Gustafson (1973, 1975,1976) and others (Bergh 1975b, Knight 1980; Popenoe 1924; Ruehle 1958; Schroeder 1958; Williams 1976). Several reviews have been published on various aspects of the avocado, including its botany (Knight 1980; Purseglove 1968; Williams 1976), breeding (Bergh 1975b), horticulture (Hodgson 1930b; Ruehle 1958; Ryerson et al. 1924), cultivars (Campbell and Malo 1976), and pollination (Gustafson and Bergh 1966). A recent collection of papers discussing the horticultural aspects of avocado production is found in Sauls et al. (1976).