Biomimetic metal organic frameworks mediated hybrid multi enzyme mimic for tandem catalysis
Abstract The combination of chemo and enzyme catalysis for multi step tandem reactions has presented great challenges for decades. Herein, glucose oxidase (GOx) and a metal organic framework MOF 545(Fe) which possessed peroxidase like activity, were combined to construct a mimic multi enzyme system for tandem catalysis. MOF 545(Fe) not only served as a support for the enzyme immobilization, but also contributed its catalytic activity to co operate with natural enzymes for the cascade reactions. Based on the prepared mimic multi enzyme system GOx@MOF 545(Fe) a colorimetric biosensor for glucose rapid detection was achieved with a low limit of detection (0.28 mM) and high specificity. Meanwhile, due to the protection of MOF 545(Fe) the immobilized enzyme exhibited excellent reusability and stability, e.g. long term stability, thermal stability and anti organic solvent stability. After being stored at room temperature for 7 days, the immobilized enzyme retained 92% of activity, while only 40% of activity of free enzyme was retained. And the immobilized enzyme maintained 71% of its initial activity after 5 cycles. This strategy of combining MOF mimics and natural enzymes for tandem catalysis may provide a novel and facile approach to design a highly efficient, stable, functional chemo enzyme composites catalyst, which holds great promise for applications in biosensing, biopharmaceutical and industrial catalysis.