Establishment of Two dimensional Polyacrylamide Gel Electrophoresis for Follicular Fluid Proteomics
Human follicular fluid(hFF),which is generated in the process of follicle formation,contains a vast variety of substances.As a medium of oocyte growth and differentiation,it directly or indirectly affects the fertility and developmental potential of oocytes.Through adjusting and optimizing 2 DE technical conditions,this study has established a relatively stable hFF protein 2 DE technology with stability and reproducibility,9 protein spots in a silver stained gel of 2 DE of human follicular fluid were analyzed and identified,among which four types are newly identified proteins expressed in follicular fluid.The results demonstrated that it is expectably to lay a foundation for further proteomics research.