Analysis of key microbial community during the start up of anaerobic ammonium oxidation process with paddy soil as inoculated sludge.
A sequencing batch reactor (SBR) anaerobic ammonium oxidation (anammox) system was started up with the paddy soil as inoculated sludge. The key microbial community structure in the system along with the enrichment time was investigated by using molecular biology methods (e.g. high throughput 16S rRNA gene sequencing and quantitative PCR) Meanwhile, the influent and effluent water quality was continuously monitored during the whole start up stage. The results showed that the microbial diversity decreased as the operation time initially and increased afterwards, and the microbial niches in the system were redistributed. The anammox bacterial community structure in the SBR anammox system shifted during the enrichment, the most dominant anammox bacteria were CandidatusJettenia. The maximum biomass of anammox bacteria achieved 1.68x109copies/g dry sludge during the enrichment period, and the highest removal rate of TN achieved around 75%
Journal of environmental sciences