Anti Inflammatory Effects of Cumin Essential Oil by Blocking JNK, ERK, and NF kB Signaling Pathways in LPS Stimulated RAW 264.7 Cells
Cumin seeds (Cuminum cyminum L. have been commonly used in food flavoring and perfumery. In this study, cumin essential oil (CuEO) extracted from seeds was employed to investigate the anti inflammatory effects in lipopolysaccharide (LPS stimulated RAW 264.7 cells and the underlying mechanisms. A total of 26 volatile constituents were identified in CuEO by GC MS, and the most abundant constituent was cuminaldehyde (48.773% Mitochondrial respiration dependent 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium (MTT) reduction assay demonstrated that CuEO did not exhibit any cytotoxic effect at the employed concentrations (0.0005 0.01% Real time PCR tests showed that CuEO significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS) cyclooxygenase (COX 2) interleukin (IL 1, and IL 6. Moreover, western blotting analysis revealed that CuEO blocked LPS induced transcriptional activation of nuclear factor kappa B (NF kB) and inhibited the phosphorylation of extracellular signal regulated kinase (ERK) and c Jun N terminal kinase (JNK) These results suggested that CuEO exerted anti inflammatory effects in LPS stimulated RAW 264.7 cells via inhibition of NF kB and mitogen activated protein kinases ERK and JNK signaling; the chemical could be used as a source of anti inflammatory agents as well as dietary complement for health promotion.
Evidence based complementary and alternative medicine eCAM