DEVELOPMENT OF NANOSTRUCTURED LIPID CARRIER FORMULATION CONTAINING OF RETINYL PALMITATE
Objective: The purpose of this research is to develop a Nanostructure Lipid Carrier (NLC) formulation containing of Retinyl Palmitate. Methods: Preparation of NLC was carried out by ultrasonication method. The formulas of NLC were developed by using virgin coconut oil and oleic acid as a liquid lipids, cetyl palmitate, and stearic acid as solid lipids, Tween 80 and Poloxamer as a surfactant and glycerine as co surfactant. Characterization of NLC consisted of visual appearance, morphology, particle size, polydispersity index (PI) and physical stability test using freeze thaw, centrifugation, and accelerate stability test method. Results: Obtained NLC revealed a good characterization with the formulation of 7.2% of cetyl palmitate, 4.8% of oleic acid, 10% of Tween 80, 10% of glycerin, and 2% of retinyl palmitate. This NLC has a pale yellow appearance, globule size of 258+ 15.85 nm; and a polydispersity index of 0.31+ 0.09. It was also physically stable after centrifuged at 13,000 rpm for 30 min, during 4 cycles of freeze thaw and storage at room temperature for 28 d. During storage for 28 d, retinyl palmitate in NLC had been degraded only about 15% in comparison with macroemulsion destroyed almost 50% of retinyl palmitate in the same time. Conclusion: NLC formulations with 7.2% cetyl palmitate, 4.8% of oleic acid, 10% of Tween 80, 10% of glycerol, and 2% of retinyl palmitate is the most optimal formula that showed a good characteristic. Stability study revealed that NLC provided better stability than macroemulsion. Keywords: Retinyl palmitate, NLC, Ultrasonication Methode