Modification of polydopamine coated Fe3O4 nanoparticles with multi walled carbon nanotubes for magnetic m dispersive solid phase extraction of antiepileptic drugs in biological matrices
AbstractIn this study, multi walled carbon nanotubes were coated on the surface of magnetic nanoparticles modified by polydopamine. The synthesized composite was characterized and applied to magnetic m dispersive solid phase extraction of oxcarbazepine (OXC) phenytoin (PHT) and carbamazepine (CBZ) from human plasma, urine, and cerebrospinal fluid samples prior to analysis by a high performance liquid chromatography photodiode array detector. The extraction parameters were investigated and the optimum condition was obtained when the variables were set to the following: sorbent type, Fe3O4@polyDA MWCNTs (length 2 mm) sample pH, 6; amount of sorbent, 15 mg; sorption time, 1.5 min at room temperature; type and volume of the eluent, 2.5 mL methanol; and salt content, none added. Under the optimized conditions, the calibration curves are linear in the concentration range 2 2000 ng/mL, the limits of detection are in the range 0.4 3.1 ng/mL, and the relative standard deviations and relative recoveries of plasma (spiked at 200 ng/mL) and CSF (spiked at 50 ng/mL) are in the ranges 1.4 8.2% and 92.8 96.5% respectively. The applicability of the method was successfully confirmed by extraction and determination of OXC, PHT, and CBZ in biological matrices. Graphical abstractMagnetic multi walled carbon nanotube core shell composites were applied as magnetic m dispersive solid phase extraction adsorbents for determination of antiepileptic drugs in biological matrices.
Analytical and Bioanalytical Chemistry