Determination of acute lethal doses (LD50 and LC50) of imidacloprid for the native bee Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae)
The bee species Melipona scutellaris (L. is native to Brazil, stingless and belongs to the Apidae family. In Brazil, stingless bees are responsible for 40 to 90% of tree species pollination, depending on the considered ecosystem. However, their survival has been threatened since the country has been standing out as the biggest pesticides consumer in the world. Many of the pesticides used are considered toxic to bees, including imidacloprid (all existing studies were conducted with the Apis mellifera (L. species) Although the bees are not the target of these substances, they are highly vulnerable to contamination. Thereby, the objective of this study was to establish the mean lethal dose (LD 50 and the mean lethal concentration (LC 50 of imidacloprid in the M. scutellaris fodder. In order to carry out this experiment, bees were collected and the test was performed according to OECD's protocol (1998a, 1998b) developed for A. mellifera For the determination of LD 50 and LC 50 data was analyzed through the Probit method, using the BioStat software. The topical LD 50 established in this study was 2.41 ng/bee for 24 hours and 1.29 ng/bee for 48 hours. The oral LC 50 was 2.01 ng i.a./mL for 24 hours and 0.81 ng i.a./mL for 48 hours. This data showed that native bees of M. scutellaris species are more sensitive to this pesticide than A. mellifera. Thus, it is important to establish management methods which take this higher susceptibility into account to protect native species.