Genome wide motif predictions of BCARR box in the amino acid repressed genes of Lactobacillus helveticus CM4
BackgroundA BCARR (branched chain amino acid responsive repressor) identified in proteolytic gene expressions in Lactobacillus helveticus is considered to negatively control transcriptions by binding to operator sites at the promoter regions in the presence of BCAAs. However, the distributions and regulatory potential of the BCARR in all genes repressed by BCAAs in CM4 remains unclear.ResultsA genome wide search for the BCARR box was conducted to clarify the contribution of BCARR in the regulation of amino acid metabolism in L. helveticus CM4. Among all 2174 genes of CM4, 390 genes repressed by amino acids were selected for the search of the BCARR box. The annotated 33 genes among the 67 predicted BCARR boxes were mainly linked to amino acid metabolism. The BCARR boxes were mainly located adjacent to the 35 sequence of the promoter; however, the repressive effects in different locations were similar. Notably, the consensus BCARR box motif, 5' A1A2A3A4A5W6N7N8N9W10T11T12W13T14T15 3' observed in highly repressed genes, revealed more frequent A T base pairing and a lower free energy than that in lowly repressed genes. A MEME analysis also supported the lower frequency of T at positions 12, 14, 13 and 15 in the BCARR box sequence of the lowly repressed gene group. These results reveal that genes with a more stable palindromic structure might be preferable targets for BCARR binding and result in higher repressions in the target gene expressions.ConclusionsOur genome wide search revealed the involvement of the proteolytic system, transporter system and some transcriptional regulator systems in BCARR box regulation in L. helveticus CM4.
BMC Microbiology