Molecular Epidemiology of Clinical Isolates of Imipenem resistant Acinetobacter baumannii from Intensive Care Unit
OBJECTIVE To investigate the clonal relationship among the isolates of imipenem resistant Acinetobacter baumannii(IRAB) from intensive care unit and from related environment analyzing with molecular methods and to track the source and spread approach of microbial infections and contamination of IRAB.METHODS Typing the IRAB strains isolated from patients and related environment by genomic DNA using automated DiversiLab (repetitive sequence based polymerase chain reaction,Rep PCR),to identify those strains and test the drug susceptibility by auto microbial analysis system (VITEK 2 Compact).RESULTS Twenty four strains of IRAB isolated from patients and ten strains of A.baumannii (ABA) isolated from environment.The resistant rate of strains of ABA isolated from environment was lower than from clinics(P0.05).Eight of 26 IRAB strains with type A,the major type,were distributed in the second section (5 from patients;1 from environment) and the first section of ICU (3 strains);The second major type was type C which was distributed in the third section (3 from patients;1 from environment).CONCLUSION There is a cross transmission of IRAB between patients and environment in ICU.It is necessary to strengthen the surveillance of ABA,and cut off the spread approach to prevent the outbreak of IRAB.