Determination of 17 Characteristic Ingredients of Plant Extracts in Hair Growth Cosmetics by Ultra High Performance Liquid Chromatography
A method was developed for the simultaneous determination of 17 characteristic ingredients of plant extracts,including paeoniflorin,hydroxysafflor yellow A,calycosin 7 glucoside ferulic acid,etc.,in hair growth cosmetics using ultra high performance liquid chromatography( UPLC) Different cosmetic samples were extracted by ultrasonic assisted extraction with the solvent of methanol water( 4: 1,V V) solution. After demulsified by the addition of appropriate amount of Na Cl and high speed centrifugation,the supernatant was transferred and analyzed with UPLC. The separation was conducted on a Waters reversed phase column of ACQUITY UPLC CSH C18( 50 mm x 2. 1 mm,1. 7 mm),and the mobile phases were methanol and the solution of 0. 05% phosphate in water. The detection was performed with a photodiode array( PDA) detector.The linear range was 0. 2 25 mg L with correlation coefficients higher than 0. 999. The limits of detection were within 0. 3 1. 5 mg kg,and the limits of quantification were from 1. 0 to 4. 0 mg kg. The average recoveries of 17 characteristic ingredients were within 93. 5% 105. 0%,with the intra and inter day precision( n 6) less than 4. 6% This method was simple,rapid,with good repeatability,and had been applied to the analysis of real samples.