Celery Seed Extract Blocks Peroxide Injury in Macrophages via Notch1/NF kB Pathway.
Oxidized low density lipoprotein (ox LDL) induced macrophage foam cell formation and injury is one of the major atherogenic factors. This study is aimed to investigate the protective effect of celery seed extract (CSE) on ox LDL induced injury of macrophages and the underlying signaling pathway. RAW264.7 macrophages were pre incubated with CSE for 24 h, followed by stimulation with ox LDL. Oil red O staining and enzymatic colorimetry indicated CSE significantly lessened lipid droplets and total cholesterol (TC) content in ox LDL injured macrophages. ELISA revealed that CSE decreased the secretion of inflammatory cytokine TNF a and IL 6 by 12 27% and 5 15% respectively. MTT assay showed CSE promoted cell viability by 16 40% Cell apoptosis was also analyzed by flow cytometry and laser scanning confocal microscope and the data indicated CSE inhibited ox LDL induced apoptosis of macrophages. Meanwhile, western blot analysis showed CSE suppressed NF kBp65 and notch1 protein expressions stimulated by ox LDL in macrophages. These results suggest that CSE inhibits ox LDL induced macrophages injury via notch1/NF kB pathway.
The American journal of Chinese medicine