Phase contrast time lapse microscopy datasets with automated and manual cell tracking annotations
Phase contrast time lapse microscopy is a non destructive technique that generates large volumes of image based information to quantify the behaviour of individual cells or cell populations. To guide the development of algorithms for computer aided cell tracking and analysis, 48 time lapse image sequences, each spanning approximately 3.5 days, were generated with accompanying ground truths for C2C12 myoblast cells cultured under 4 different media conditions, including with fibroblast growth factor 2 (FGF2) bone morphogenetic protein 2 (BMP2) FGF2 BMP2, and control (no growth factor) The ground truths generated contain information for tracking at least 3 parent cells and their descendants within these datasets and were validated using a two tier system of manual curation. This comprehensive, validated dataset will be useful in advancing the development of computer aided cell tracking algorithms and function as a benchmark, providing an invaluable opportunity to deepen our understanding of individual and population based cell dynamics for biomedical research.
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