Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet excitable yellow fluorescing variant of Aequorea victoria GFP, we developed dual FRET based caspase 3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.
Nature Methods