Dummy molecularly imprinted polymer based microplate chemiluminescence sensor for one step detection of Sudan dyes in egg.
The objective of this study is to report a molecularly imprinted polymer based chemiluminescence method for determination of Sudan dyes. A dummy template molecularly imprinted polymer capable of recognizing seven Sudan dyes was first synthesized and its recognition mechanism was studied by using computation simulation method. The polymer was coated in the wells of conventional microplate to prepare a chemiluminescence sensor and the assay process consisted of only one sample loading step prior to signal acquisition. The optimized sensor was used to determine the seven dyes in egg yolk and the results were confirmed with a high performance liquid chromatography. Results showed that this sensor achieved ultrahigh sensitivity (1.0 5.0 pg/mL) rapid assay process (10 min) and satisfactory recovery (70.5% 92.2% Furthermore, the sensor could be reused for 5 times. Therefore, this sensor could be used as a useful tool for screening the residues of Sudan dyes in egg.
Food chemistry