An In Vitro Comparative Evaluation of Antimicrobial Efficacy of Propolis, Morinda Citrifolia Juice, Sodium Hypochlorite and Chlorhexidine on Enterococcus faecalis and Candida albicans.
AIM To evaluate the antimicrobial effectiveness of Propolis, Morinda citrifolia juice, Sodium hypochlorite and Chlorhexidine onEnterococcus feacalis (E. feacalis) and Candida albicans (C. albicans) as endodontic irrigants. MATERIALS AND METHODS Four clinical isolates and one standard ATCC sample (29212) of E. feacalis and ATCC sample (90028) of C. albicans were inoculated into 5ml of peptone water each and incubated at 37deg C for 3 to 4 hours to attain the turbidity corresponding to 0.5 McFarland standard CFU. We followed Disc and well diffusion Kirby Bauer method to attain the zones of inhibition. RESULTS Overall comparison of reagents revealed a significant difference among zones of inhibition. The standard concentration of 5% sodium hypochlorite, 2% chlorhexidine, 10% Propolis and 100% Morinda citrifolia juice illustrated the maximum inhibition zone for both test organisms. CONCLUSION All four reagents had an antimicrobial effect on the microorganisms tested. Sodium hypochlorite and Chlorhexidine were more effectual than Propolis and Morinda citrifolia juice and there was increased antimicrobial efficacy with increasing concentrations. CLINICAL SIGNIFICANCE A study for finding safe herbal agents that can be used as endodontic irrigants revealed that Propolis, Morinda citrifolia juice has antibiotic properties and can replace routinely used agents thereby limiting the side effects.
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