Adipose derived stem cells therapy effectively attenuates PM2.5 induced lung injury
Background The adverse health effects of fine particulate matter (PM 2.5 exposure are associated with marked inflammatory responses. Adipose derived stem cells (ADSCs) have immunosuppressive effects, and ADSC transplantation could attenuate pulmonary fibrosis in different animal disease models. However, whether ADSCs affect PM 2.5 induced lung injury has not been investigated. Method C57BL/6 mice were exposed to PM 2.5 every other day via intratracheal instillation for 4 weeks. After that, the mice received tail vein injections of ADSCs every 2 weeks. Results ADSC transplantation significantly attenuated systemic and pulmonary inflammation, cardiac dysfunction, fibrosis, and cell death in PM 2.5 exposed mice. RNA sequencing results and bioinformatic analysis suggested that the downregulated differentially expressed genes (DEGs) were mainly enriched in inflammatory and immune pathways. Moreover, ADSC transplantation attenuated PM 2.5 induced cell apoptosis and pyroptosis in the lungs and hearts. Conclusion ADSCs protect against PM 2.5 induced adverse health effects through attenuating pulmonary inflammation and cell death. Our findings suggest that ADSC transplantation may be a potential therapeutic approach for severe air pollution associated diseases.
Stem cell research therapy