Casitone dependent transcriptional regulation of theprtP andprtM genes in the natural isolateLactobacillus paracasei subsp.paracasei
TheprtP prtM intergenic region ofLactobacillus paracasei subsp.paracasei BGHN14 was cloned and sequenced. The nucleotide sequence of theprtP prtM intergenic region in BGHN14, containing divergently orientated PprtP and PprtM promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone dependent transcriptional regulation of the lactococcalprt genes was not found in the BGHN14. The activity of PprtM inL. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas PprtP was completely inactive. WhenL. casei ATCC393T was used as host, both PprtP and PprtM were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone dependent regulation of theprt genes in BGHN14 and lactococci are different.
Folia Microbiologica