Ultrasensitive dual signal ratiometric electrochemical aptasensor for neuron specific enolase based on Au nanoparticles@Pd nanoclusters poly(bismarck brown Y) and dendritic AuPt nanoassemblies
Abstract Neuron specific enolase (NSE) is viewed as a tumor marker of small cell lung cancer (SCLC) with high diagnostic sensitivity and specificity, whose facile and sensitive detection is critical for clinical diagnosis. In this work, core shell Au nanoparticles @Pd nanoclusters poly(bismarck brown Y) (Au@Pd P(BBY) and hierarchically dendritic AuPt nanoassemblies (AuPt NAs) were synthesized independently by a one pot aqueous method. A dual signal ratiometric electrochemical aptasensor was developed for ultrasensitive determination of NSE based on ferrocene grafted Au@Pd P(BBY) (Fc g Au@Pd P(BBY) and reduced graphene oxide/thionine decorated AuPt NAs (rGO/Thi/AuPt NAs) Under optimal conditions, the changes in the peak currents linearly correlate to the NSE concentrations in the wide range of 0.0001 50.0 ng mL 1 with ultralow detection limit (0.03 pg mL 1, S/N 3) The aptasensor was explored in a diluted human serum sample with acceptable results, showing its great potential in clinical diagnosis and treatment.