Targeted delivery and apoptosis induction of trans resveratrol ferulic acid loaded chitosan coated folic acid conjugate solid lipid nanoparticles in colon cancer cells.
The present study is aimed to study and to evaluate the colon cancer targeting efficacy of chitosan coated trans resveratrol (RSV) and ferulic acid (FER) loaded SLNs (solid lipid nanoparticles) that conjugated with folic acid (FA) (C RSV FER FA SLNs) in suitable models (in vitro) The FA conjugation is performed using co encapsulation method of stearic acid. Similarly, the prepared SLNs are exhibited better stability even under acidic conditions to exhibit their potentials to use as drug delivery system. Further, the optimized formulations (SLNs) are tested for physiochemical characterizations, which include FTIR, XRD, 1HNMR, particle size, zeta potential, and drug release. In vitro anti cancer studies using HT 29 cells including, fluorescence staining, flow cytometry, and western blot analysis revealed that the C RSV FER FA SLNs effectively involved and increased cytotoxicity in cancer cells that leads to induction of apoptosis as compared to free RSV FER. Thus, it is reported that, the good stability under acidic conditions of this C RSV FER FA SLNs may serve as a promising candidate for novel nanodrug formulations in cancer therapy.
Carbohydrate polymers