Interaction of glycyrol with calcineurin A studied by spectroscopic methods and docking
We have shown previously that glycyrol has an inhibitory effect on the immune response in mice by reducing calcineurin activity (Li et al. 2010, Pharm Biol 48:1177 1184) Here, we investigated the interaction of glycyrol with calcineurin A (CNA, catalytic A subunit of calcineurin) by spectroscopic methods and docking. We showed that glycyrol binds to CNA via hydrophobic interactions in a ratio of 1:1, and the main binding site is in the catalytic domain of CNA close to the calcineurin B subunit binding domain. Binding of glycyrol changes the secondary structure of CNA, and this effect may possibly inhibit CN activity. (c) 2011 IUBMB IUBMB Life, 63(1) 14 20, 2011
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