Lactobacillus Salivarius REN Inhibits Rat Oral Cancer Induced by 4 Nitroquioline 1 Oxide
Despite significant advances in cancer therapy, cancer related mobility and mortality are still rising. Alternative strategies such as cancer prevention thus become essential. Probiotics represent an emerging option for cancer prevention, but studies are limited to colon cancers. The efficiency of probiotics in the prevention of other cancers and the correlative mechanism remains to be explored. A novel probiotics Lactobacillus salivarius REN (L. salivarius REN) was isolated from centenarians at Bama of China, which showed highly potent antigenotoxicity in an initial assay. 4 nitroquioline 1 oxide (4NQO) induced oral cancer model was introduced to study the anticancer activity of L. salivarius REN in vivo. The results indicated that oral administration of probiotic L. salivarius REN or its secretions could effectively suppress 4NQO induced oral carcinogenesis in the initial and postinitial stage, and the inhibition was in a dose dependent manner. A significant decrease of neoplasm incidence (65% 0% was detected in rats fed with the high dose of L. salivarius REN [5 x 1010 CFU/kg body weight (bw)/d] In vivo evidences indicated that the probiotics inhibited 4NQO induced oral cancer by protecting DNA against oxidative damage and downregulating COX 2 expression. L. salivarius REN treatment significantly decreased the expression of proliferating cell nuclear antigen (PCNA) and induced apoptosis in a dose dependent manner. Our findings suggest that probiotics may act as potential agents for oral cancer prevention. This is the first report showing the inhibitory effect of the probiotics on oral carcinogenesis. Cancer Prev Res; 6(7) 686 94. (c)2013 AACR.
Cancer Prevention Research