Green synthesis of silvernano particles from Artemisia pallens, Wall. ex DC. and its cytotoxicity activity as an anti mycobacterial agent
In present investigation, the synthesis of silver nanoparticles from of Artemisia pallens, were studied for Antimycobacterial activity against three species as Mycobacterium tuberculosis (MTCC 300) M.pheli (MTCC 1724) M.avim (MTCC 1723) The Anti mycobacterial activity of silver nanoparticles from A. pallens was evaluated by agar cup well plate method using different dilutions such as 10 mg/ml, 20 mg/ml and 30 mg/ml. These extracts showed maximum activity at 30mg/ml. Among the strains, the maximum zone of inhibition was noted against Mycobacterium tuberculosis (32.5mm) and Mycobacterium spegmatis (29.4mm) and Mycobacterium pheli (29.4mm) respectively. The Minimum inhibitory concentration (MIC) of silver nanoparticles for A.pallens showed against two species of Mycobacterium like Mycobacterium tuberculosis as (230 mg /mL and Mycobacterium pheli (230 mg /mL) .These results showed that the silver nanoparticles from A.pallens is an effective Anti mycobacterial agent. The cytoxicity was also done by hemolytic assay Key words: Artemisia pallens Wall. ex DC (AP) AgNPs, Anti mycobacterial, MIC, Hemolytic assay