Biochemical characterization of bacteria isolated from different wound samples
Abstract: This study was conducted with an aim to isolate and characterize the bacteria from different wound samples obtained from a local Hospital at Jalandhar, India. The samples were transported to the Microbiology Laboratory, D.A.V University, Jalandhar from the hospital as soon as they were obtained. The microorganisms isolated from these samples were cultured using different media such as Nutrient agar (NA) MacConkey agar (MAC) Mueller Hinton agar (MHA) and Mannitol salt agar (MSA) The spread plate and streaking techniques were used for obtaining pure cultures. The bacteria were morphologically identified by their colour, colony appearance, and gram's staining technique. A total of 13 morphotypes were isolated from five different types of wound samples. The results showed the maximum number of morphotypes obtained were gram positive. The bacteria were further characterized using different biochemical tests. This study concludes that all types of the wound have different bacterial fauna which may be pathogenic and pose a serious health risk if not taken care of.