Eight microsatellite loci in Clark's anemonefish, Amphiprion clarkii
AbstractTo determine the genetic relationship between different colour morphs (orange and blackmorphs) of Clark's anemonefish Amphiprion clarkii in Taiwan, we isolated eight poly morphic microsatellite loci. A large number of alleles (range, 6 30) and high levels ofobserved heterozygosity (range, 0.1231 0.8358) were resolved in 71 individuals from twopopulations, indicating that these markers should be useful in assessing the relationshipbetween the two colour morphs of A. clarkii Keywords Amphiprion clarkii anemonefish, microsatellites Received 21 January 2007; revision accepted 4 April 2007 The colouration pattern of tropical reef fish has often beenused for taxonomic purposes. Nonetheless, the roles ofsexual selection and colour patterns on genetic different iation and speciation are not completely understoodamong reef fishes. Only a few studies have focused on thephylogenetic relationship between closely related specieswith different colour morphs (McMillan et al 1999; Bernardi