Porous collagen hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration.
Current bone grafting materials have significant limitations for repairing maxillofacial and dentoalveolar bone deficiencies. An ideal bone tissue engineering construct is still lacking. The purpose of the present study was first to synthesize and develop a collagen hydroxyapatite (Col HA) composite through controlled in situ mineralization on type I collagen fibrils with nanometer sized apatite crystals, and then evaluate their biologic properties by culturing with mouse and human mesenchymal stem cells (MSCs) We synthesized Col HA scaffolds with different Col:HA ratios. Mouse C3H10T1/2 MSCs and human periodontal ligament stem cells (hPDSCs) were cultured with scaffolds for cell proliferation and biocompatibility assays. We found that the porous Col HA composites have good biocompatibility and biomimetic properties. The Col HA composites with ratios 80:20 and 50:50 composites supported the attachments and proliferations of mouse MSCs and hPDSCs. These findings indicate that Col HA composite complexes have strong potentials for bone tissue regeneration.
The Journal of oral implantology