The derivatization and antioxidant activities of yeast mannan.
The alkaline mannan (M) was extracted from yeast cell walls, and the best extraction condition was: 100degC, 2h, 1% NaOH, solid liquid ratio of 10:1. The purity of mannan was 96.15% Its five derivatives were obtained by chemical modification methods, and they were: phosphorylated mannan (P M) sulfated mannan (S M) carboxymethylated mannan (CM M) carboxymethylated phosphorylated mannan (CMP M) and carboxymethylated sulfated mannan (CMS M) The phosphate substitution degree of P M and CMP M was 0.2 and 0.1, respectively. The sulfate substitution degree of S M and CMS M was 0.67 and 0.65, respectively. The carboxymethyl substitution degree of CM M, CMP M and CMS M were 1.11, 1.17 and 0.25, respectively. It indicated that the hydroxyl radical scavenging capacities of P M and CMP M were 15% higher than that of M. The anti lipid peroxidation capacity of all derivatives increased than that of M, especially for CMP M, its scavenging effect was 25% higher.
International journal of biological macromolecules