Butanediol production from glycerol and glucose by Serratia marcescens isolated from tropical peat soil
Abstract Six bacterial strains, Bacillus subtilis LQOB SE1, Bacillus coagulans LQOB SE2, Bacillus pumilus LQOB SE3, Brevibacillus brevis LQOB SE4, Lysinibacillus sp. LQOB SE5 and Serratia marcescens LQOB SE6, isolated from a tropical peat soil, were assessed for 2,3 butanediol (2,3 BD) production by fermentation of glucose and glycerol. Only S. marcescens LQOB SE6 strain was capable of producing 2,3 BD from both glucose and glycerol fermentation, and this process was optimized by changes in fermentation parameters. The best conditions for glycerol fermentation were initial pH 7.0, 8 g L 1 nutrient broth, 50 g L 1 substrate, and an incubation time of seven days, with 2,3 BD yield of 0.30 g g 1, and productivity of 0.13 g L 1 h 1. The best conditions for glucose fermentation were initial pH 7.0, 8 g L 1 nutrient broth, 75 g L 1 substrate, and an incubation time of five days, with 2,3 BD yield of 0.42 g g 1 and productivity of 0.15 g L 1 h 1. meso 2,3 butanediol was the only stereoisomer obtained from glucose fermentation, while glycerol fermentation also produced smaller amounts of (2R,3R) 2,3 butanediol.