Intramolecular Co reaction Accelerated Electrochemiluminescence of Polypeptide biomineralized Gold Nanoclusters for Targeted Detection of Biomarkers.
The development of label free electrochemiluminescence (ECL) based sensing technology for biomarkers detection has a congenital defect compare with non competitive sandwich type biosensor due to the lack of detection antibody conjugated with signal label. Nevertheless, it is still not difficult to realize ultrasensitive analysis benefit from the exploration of efficient sensing substrates and signal transducers. In this work, an innovative sensing system is purposed utilizing Fe2O3 nanoarrays (Fe2O3 NAs) as well ordered co reaction accelerator and polypeptide biomineralized gold nanoclusters (Au NCs) as signal transducer. Bifunctional peptide ligands of H2N MMYYHFRRHL COOH (MYH 10) is self designed, it can not only play a role of reductant and coupling reagent for clusters formation using MMYY sequence root in N terminal, but also act as connection for coupling carriers and immune molecules via HFRRHL region of C terminal. Additional intramolecular ECL emission between Au NCs and tris(3 aminoethyl)amine (TAEA) all strategies undoubtedly reduces the spatial hindrance of the sensing interface and increases the effectiveness of electron transfer and immune recognition. With CYFRA21 1 as target, the biosensor exhibits linear ECL response in a wide range (10 fg mL 1 100 ng mL 1) and an ultralow detection limit of 1.33 fg mL 1 (S/N=3) With convincing experimental data, these innovative strategies will be more eye catching in peptide based nanocluster synthesis and expansion of more novel thought for sensing platform fabrication.
Analytical chemistry