Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles.
In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2) glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI ATRP) and hexamethylene diamine (as a spacer arm) The p(GMA) grafted and SA modified form (i.e. Fe3O4@SiO2@pGMA SA 3) was used for covalent immobilization of invertase (EC 3.2.1.26) The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA) SA 3 was 36.1+ 0.9 and 33.4+ 1.3mg/g, respectively. The Km and Vmax values of immobilized invertase were found to be 39.4mmol/L and 349.5mmol/L min, and not significantly changed compared with free form (34.3mmol/L and 387.2mmol/Lmin) respectively, revealed that the applied protocol did not have any detrimental effect on the retained activity of immobilized invertase.
Food chemistry