Increased amounts and stability of telomeric repeat containing RNA (TERRA) following DNA damage induced by etoposide
Telomeric repeat containing RNAs (TERRAs) are long noncoding RNAs transcribed from subtelomeres toward telomeric repeat tracts, which have been implicated in telomere protection and heterochromatin formation. Genotoxic stress leads to upregulation of TERRAs. However, the mechanism of DNA damage mediated TERRA induction remains elusive. Here, we treated HeLa cells with etoposide, a DNA double strand break generating agent, for various times and monitored the levels of TERRAs. Etoposide treatment led to a gradual time dependent increase in TERRAs. Etoposide mediated induction was evident in many TERRAs arising from various chromosome loci, including 20q and XpYp. Chromatin immunoprecipitation assays revealed no significant changes in the occupancy of RNA polymerase II at telomeres upon etoposide treatment. Interestingly, TERRAs arising from 20q, XpYp, 10q, and 13q degraded at slower rates in cells treated with etoposide, while degradation rates of TERRAs from many loci tested were nearly identical in both etoposide and mock treated cells. Telomere damage occurred from early time points of etoposide treatment, but telomere lengths and abundance of telomeric repeat binding factor 2 (TRF2) at telomeres remained unchanged. In summary, etoposide treatment led to telomere damage and TERRA accumulation, but telomere lengths and TRF2 mediated telomere integrity were maintained. Etoposide mediated TERRA accumulation could be attributed partly to RNA stabilization. These findings may provide insight into the post transcriptional regulation of TERRAs in response to DNA damage.
PloS one