b Sitosterol 2 hydroxypropyl b cyclodextrin inclusion complex: Characterization and inhibitory effect on adipogenesis in 3T3 L1 pre adipocytes
HIGHLIGHTSSit HP &bgr; CD inclusion complex was prepared using the grinding method.The formation of Sit HP &bgr; CD complex was confirmed by DSC, TLC, NMR, and ROESY.Sit HP &bgr; CD complex reduced intracellular lipid accumulation in 3T3 L1 cells.Sit HP &bgr; CD complex had a higher inhibitory effect on adipogenesis than Sit. ABSTRACT &bgr; Sitosterol (Sit) has been used as a functional food additive. Among its many beneficial effects, this phytosterol plays a role in controlling obesity by inhibiting the adipogenesis process of pre adipocytes. However, the highly lipophilic character of Sit limits its bioavailability. In the present study, 2 hydroxypropyl &bgr; cyclodextrin (HP &bgr; CD) was used to form a complex with Sit, namely the Sit HP &bgr; CD inclusion complex, and the inhibitory effect of this complex on adipogenesis in the 3T3 L1 pre adipocyte cell line was investigated. The results of DSC, TLC, 1H NMR spectroscopy, and 2D ROESY showed that the Sit HP &bgr; CD inclusion complex was successfully synthesized. In addition, the inhibitory effect of the Sit HP &bgr; CD inclusion complex on adipogenesis was evaluated using the Oil Red O staining method and western blot analysis after a 14 day adipogenesis induction in 3T3 L1 pre adipocytes. The results showed that the Sit HP &bgr; CD inclusion complex had a higher efficiency than Sit in reducing intracellular lipid accumulation and the expression levels of PPAR&ggr; and FAS in 3T3 L1 cells, suggesting that the inhibitory effect on adipogenesis was improved by the formation of the Sit and HP &bgr; CD complex.
Steroids