Western Blot Techniques.
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA) a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.
Methods in molecular biology