Angelica gigas Nakai extract loaded fast dissolving nanofiber based on poly(vinyl alcohol) and Soluplus for oral cancer therapy.
A poly(vinyl alcohol) (PVA) and Soluplus (SP) based nanofiber (NF) mat was fabricated using an electrospinning method for the delivery of Angelica gigas Nakai (AGN) extract (ext) to oral cancers. AGN/SP NF (mean diameter: 75+ 26nm; entrapment efficiency: 84.6+ 18.6% and AGN/PVA/SP NF (mean diameter: 170+ 35nm; entrapment efficiency: 81.0+ 10.1% were fabricated using an electrospinning method. Amorphization of AGN EtOH ext was verified by X ray diffractometry (XRD) analysis during the electrospinning process for the fabrication of NF structures. The AGN/PVA/SP NF group exhibited instant wetting (within 2s) and rapid disintegration (within 3min) properties compared with those in the AGN/PVA NF group, assuring the successful and conventional application of AGN/PVA/SP NF film in the oral cavity without the intake of beverages. After the spontaneous dispersion of NF in the aqueous media, it was converted to nanoparticles with a narrow size distribution. In YD 9 cells (oral squamous cell carcinoma from buccal cheek) the anti proliferation activity was ordered as follows: AGN EtOH ext suspension AGN/PVA NF AGN/PVA/SP NF. All of these findings indicated that AGN/PVA/SP NF can be used as a fast dissolving mat formulation for the therapy of oral cancers.
International journal of pharmaceutics