The effect of trans 10, cis 12 conjugated linoleic acid on gene expression profiles related to lipid metabolism in human intestinal like Caco 2 cells
We conducted an in depth investigation of the effects of conjugated linoleic acid (CLA) on the expression of key metabolic genes and genes of known importance in intestinal lipid metabolism using the Caco 2 cell model. Cells were treated with 80 mmol/L of linoleic acid (control) trans 10, cis 12 CLA or cis 9, trans 11 CLA. RNA was isolated from the cells, labelled and hybridized to the Affymetrix U133 2.0 Plus arrays (n 3) Data and functional analysis were preformed using Bioconductor. Gene ontology analysis (GO) revealed a significant enrichment (P 0.0001) for the GO term lipid metabolism with genes up regulated by trans 10, cis 12 CLA. Trans 10, cis 12 CLA, but not cis 9, trans 11 CLA, altered the expression of a number of genes involved in lipid transport, fatty acid metabolism, lipolysis, b oxidation, steroid metabolism, cholesterol biosynthesis, membrane lipid metabolism, gluconeogenesis and the citrate cycle. These observations warrant further investigation to understand their potential role in the metabolic syndrome.
Genes Nutrition