A click chemistry based microRNA maturation assay optimized for high throughput screening.
Catalytic enzyme linked click chemistry assays (cat ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat ELCCA to include the kinetically superior inverse electron demand Diels Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high throughput screening.
Chemical communications