RAPID DETECTION AND GENOTYPING OF HUMAN PAPILLOMAVIRUS IN THE TUMOR TISSUE OF THE ANO-GENITAL REGION
More than 77 types of human papillomavirus (HPV) have been found up to now, among which at least 30 types are associated with uro-genital tumors. So the detection and genotyping would be of great importance for studying the etiology and prognosis of the uro-genital tumors. However, HPV has great heterogeneity, so the detection of HPV type in an unknown specimen with routine diagnostic techniques would be somewhat difficult. Hence we used the reverse dot blot (RDB) technique to detect and genpotype the infecting HPVs. Seven sequence-specific oligonucleotide probes, corresponding respectively to 7 types of HPV(HPV6B, 11,16,18,31,33,,and 35) were used. After the synthesis of these 7 types of probes, they were fixed successively in order on a strip of nylon membrane to form 7 spots, then they were hybridized with the PCR products of the specimen DNA sequences. The result is that any one of the 7 types of HPV DNA, if positive, can be identified on one nylon strip. We collected 177 specimens from the hospitals in Guangzhou (except that 20 cases of esophageal carcinoma were from Henan Province). RDB technique was used to detect and genotype the infecting HPVs and the results were as follows: Uterine cervical carcinoma 86 cases, including 68 squamous carcinomas and 18 adenocarcinomas, the HPV positive numbers were 59/68 (86.76%) of the former (47 for type 16, 9 for type 18, 3 for type 16, 18 mixed infection) and 8/18 (44.44%) of the latter (5 for type 18, 3 for type 16); condyloma acuminatum 42 cases with 39 HPV positive ones (92.86%) of which 17 were type 6B, 20 of type 11 and 2 of mixed infection of types 6B and 11;chronic cervicitis 29 cases, with only one HPV positive (3.45%); and finally esophageal carcinoma 20 cases, with 7 HPV positive ones (35.00%).