Application of LFS in Sugarcane Tissue Culture for Rapid Propagation
A new hormone was used for sugarcane tissue culture and rapid propagation.The stem apexes of ROC22 and its sub-cultured plantlets were used as the materials.MS basal medium supplied with different concentrations of LFS as the treatment,while 6-BA as the control in the differentiating and multiplication stage,and NAA as the control in the rooting stage,respectively.It was revealed that LFS could significantly retard the browning of the stem apexes and increase stem apexes' survival ratio.It also demonstrated that 2.5～3.0 mg/L LFS was suitable for differentiation,while 3.0～5.0 mg/L for multiplication,and 8.76 times of multiplication rate was got after three subcultures while 8.53 times for the control.Compared with NAA,LFS could induce the occurrence of adventitious roots and the appropriate concentrations were between 2.0～4.0 mg/L with the rooting rate more than 90%.LFS showed favorable application prospects in sugarcane tissue culture for rapid propagation.