Proliferation of γδT cells induced by IL-2 and bisphosphonates-treatment in patients with cancer
Objective To investigate the proliferation function of gamma delta T cells by IL-2 and nitro- gen eontaining-bisphosphonates(N-BP)-stimulated on patients with cancer.Methods We analyzed 103 patients with eaneer and 43 healthy individuals.Gamma delta T cells proliferation assay in vivo determine the absolute count of gamma delta T cells before and after treatment by flow cytometry.Gamma delta T cells proliferation assay in vitro determines the relative count of gamma delta T cells after IL-2 and N-BP-stimulated by flow cytometry. Results In patients with cancer and healthy individuals,the median of the absolute count of all T cells,gamma delta T cells and the percent of gamma delta T cells were 1306 cells/μl vs 1522 cells/μl(P=0.003),64 cells/μl vs 162 cells/μl(P0.001),5% vs 11%(P0.001).In 24 patients treated circulating the median of the ab- solute count of all T cells,gamma delta T cells and the percent of gamma delta T cells were 1283 cells/μl vs 1552 cells/μl(P=0.001),54 cells/hal vs 107 cells/μl(P0.001),6% vs 8%(P=0.008).The clinical response rate to IL-2 and N-BP-stimulated were 68.9% in all patients with cancer.Conclusion The function of gamma delta T cells proliferation is defective in the patients with cancer.The clinical response was different to IL-2 and N- BP-stimulated in differentiating patients with cancer individuals.The function of gamma delta T cells proliferation may be stimulated by IL-2 and N-BP.The gamma delta T cells proliferation assay in vitro may be applied to a tar- get of treatment by IL-2 and N-BP-stimulated.