Infectious spleen and kidney necrosis virus (ISKNV) from Siniperca chuatsi: development of a PCR detection method and new evidence of iridovirus.
A PCR based detection procedure for infectious spleen and kidney necrosis virus (ISKNV) from Siniperca chuatsi was developed by using a pair of primers which were designed from the sequence of the ribonucleotide reductase small subunit (RNRS) gene of red sea bream iridovirus (RSIV). The clone and sequence analysis of the PCR product confirmed that ISKNV is a iridovirus.