Cloning and protein property analysis of the transcription factor MYB 12 gene in woodland strawberry(Fragaria vesca)
Total RNA was extracted from leaves of woodland strawberry(Fragaria vesca) using TRIzol method. A specific primer pair was designed to amplify the MYB 12 gene encoding a transcription factor by RT-PCR. A specific fragment about 1 kb was amplified and then cloned and sequenced. Sequence analysis showed that the fragment consisted of 988 nucleotides(nts), including a complete 855 nts ORF encoding 285 amino acids. Sequence comparison revealed that MYB 12 of woodland strawberry shared a relatively high sequence similarity(75%-76%) with that of five other Rosaceae plants and a relatively low sequence similarity(47%-68%) with that of seven non-Rosaceae plants. A phylogenetic tree based on the nucleotide sequence alignment of the MYB genes was constructed. The MYB gene of woodland strawberry and five other Rosaceae plants were clustered into the same branch, which indicated that woodland strawberry had a close relationship with Rosaceae plants. Bioinformatics analyses demonstrated that MYB 12 protein was hydrophobic. Its N-terminal contained two MYB structural domains belonging to subtribe R2R3-MYB. The secondary structure of MYB 12 protein was dominated by α-helix and some random coils were included. This research will provide a basis for identifying the role of the MYB gene in regulating plant growth and development, as well as physiological and metabolic processes.