Investigation for Relationship Between Heat Treatment Process and Immunogenicity of Lysozyme in Egg By Spectroscopic Method
Thermal denaturation for lysozyme protein of egg has been investigated by using circular dichroism spectra and fluorescence spectra systematically.The corresponding immunogenicity of lysozyme protein has been tested with ELISA experiments.In addition,bioinformatics methods have also been used to investigate the spectral characteristics,corresponding immunogenicity change and conformational changes of the protein during thermal denaturation.Results from this study should be useful to the establishment of a spectral method examining the extent of thermal denaturation of the protein.