Studies on the distribution, excretion, and metabolism of α‐ and γ‐isomers of [14C] chlordane in rabbits
Abstract [C] α‐ and γ‐chlordane (about 1,700 mg of each) were orally administered to four male rabbits in four doses. Urine and feces were collected daily during the course of administration. Muscle, liver, kidney, brain, and fat were sampled from each treated rabbit. Distribution of the radioactive residues were determined in the excreta and the tissues. The rabbits excreted 77% of the a‐isomer and 82% of the γ‐isomer in the feces and in the urine. The levels of radioactive residues of the a‐isomer were in the order: fat > kidney > muscle > liver > brain; and that of the γ‐isomer were: kidney > fat > liver > muscle > brain. Oxychlordane concentration in the tissues was more than that of the parent compound and was at a maximum in the fat. Collected urine was hydrolyzed by acid and extracted with ether. The ether extract was chromatographed on a Florisil column and several fractions collected. Metabolites in the non‐polar fractions, eluted from the florisil column, were isolated by gas chromatography, and...