Identification of Host Proteins Packaged in H5N1 Avian Influenza Virus Particles by Mass Spectrometry
In this study,sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS PAGE) coupled with mass spectrometry were used to perform a comprehensive proteomics analysis of the purified influenza virus particles propagated from embryonated chicken eggs,which were purified by 20%-60% sucrose(w/w) density gradient centrifugation.In addition to the virus-encoded proteins,12 proteins originated from embryonated chicken eggs were identified in the purified virus particles,such as Annexin A2,Peptidyl-prolyl cis-trans isomerase B,Peroxiredoxin-1,Phosphoglycerate kinase,and Pyruvate kinase muscle isoenzyme.Two kinds of cytoskeletal proteins,tubulin b-3 and actin,were also detected.