Determination of the Organic Acids from the Fruit of Myrica ruba by RP-HPLC
A RP-HPLC method with a UV detector was established to determine the organic acids from the fruit of Myrica ruba.The chromatographic conditions were: Waters PhenomenexM C18(4.6 mmID×250 mm,4 μm)used for the column(at 35 ℃)with 0.04 mol·L-1 KH2PO4-H3PO4 buffer(pH2.4)as the mobile phase,and the flow rate 0.8 mL·min-1,detector wavelength UV-214 nm.The correlation coefficient of the calibration graphs was 0.999 0-0.999 9.The recovery was 88%～101%,RSD3.06%.