Cloning and Expression of Chicken Interferon-gamma Gene
A specific DNA fragment with a length of 500 bp was cloned from concanavalin A (ConA)-activated spleen lymphocytes by reverse transcription-polymerase chain reaction (RT-PCR) with a pair of designed primers. By means of endonuclease and sequencing, this fragment was identified as the gene of chicken interferon-gamma (CHIFN-γ),it was inserted into PGEX-4T-1, a prokaryotic expression plasmid, and expressed in Escherichia coli BL21, which were further induced by IPTG and cultured,and a fusion protein was obtained with about 43 000 of molecular weight in SDS-PAGE. This result showed that the cloned CHIFN-γ gene was expressed in prokaryotic cells.