Research of the correlation with pathology and kinetics of T_H1/T_H2 cells and expression of iNOS in animals of tuberculosis
Objective To investigate the correlation of pathology to immunology in animals with tuberculosis by molecular pathology. Methods BALB/c mice were randomly divided into two groups: tuberculosis model (A) and control mice (N). Immunohistochemistry technology was used to investigate the correlation between the expression of IFN γ, IL 4 and iNOS and the type as well as magnitude of tissue injury. Results Two phases were observed during the 2nd week characterized by inflammatory infiltration in the alveolar capillary interstitium and the tissues surrounding blood vessels. A predominance of T H1 cells was shown by a higher percentage of IFN γ positive cells and a lower percentage of IL 4 positive cells in the inflammatory infiltrate identified by immunohistology. From the 4 th week after infection to the end of experiment, we observed a chronic advanced phase characterized by pneumonia and local necrosis with a T H0 balance due to an equivalent proportion of IFN γ and IL 4 positive cells in the lung lesions. The iNOS expression was increased in acute phase and decreased in chronic phase. Conclusion There is a closely correlation on the kinetics of T H1/T H2 cells and iNOS expression with the type and magnitude of tissue injury during the infection of tuberculosis.