High Resolution Adsorbent of Liquid Chromatography for Protein Analysis: Cattle Bone-Originated Hydroxyapatites Characterized by Heating
The functional spherical particles of cattle-bone originated hydroxyapatite (HAp) were prepared by a spray-drying method and they exhibited considerably higher resolutions and theoretical plate numbers for chromatographic separation of albumin and lysozyme than HAp synthesized from reagents. The chromatographic functions of particles were easily regulated by changing the heat-treatment temperature of HAp, resulting from the shift of the adsorption heat for the two proteins.