Growth cycle stage-dependent NaCl induction of plasma membrane H+-ATPase mRNA accumulation in de-adapted tobacco cells
A cDNA clone encoding an isoform of the plasma membrane H+-ATPase was isolated from Nicotiana tabacum. The steady-state plasma membrane H+-ATPase message levels were the same in unadapted tobacco cells and tobacco cells adapted to 428 mol m−3 NaCl. When cells adapted to 428 mol m−3 NaCl maintained in the absence of NaCl (deadapted) for an excess of 100 passages were exposed to 400 mol m−3 NaCl for 24 h, there was an increased accumulation of plasma membrane H+-ATPase message. The NaCl responsiveness of the deadapted cells was dependent upon the growth cycle stage. Alterations in the levels of plasma membrane FT-ATPase message during the growth cycle support a role for the H+-ATPase in cell growth. These results document the induction by NaCl of plasma membrane FT-ATPase message accumulation in tobacco cells, and suggest that enhanced expression of the plasma membrane FT-ATPase has a role in the short term response of cells of NaCl, but is not necessarily involved in long-term adaptation.