Study of Interaction of Fluorescein Sodium and Bovine Serum Albumin by Capillary Electrophoresis/Frontal Analysis and Molecular Modeling Technology
The interaction between fluorescein sodium and bovine serum albumin was investigated by capillary electrophoresis/frontal analysis.Free drugs′ concentrations of fluorescein sodium in different systems of fluorescein sodium blended with BSA by different concentration ratio were known through standard curve of drug concentration and plateau peak height.The experimental data were fitted respectively by a Klotz linear and non-linear regression curve fitting program.The binding constant,the numbers of binding sites and thermodynamic parameters were obtained in the temperatures of 286 K and 294 K.The hydrophobic force was determined by thermodynamic parameters.Finally,computer software was also used to simulate the interaction.