Optimization for hydrolyzing conditions of bioactive peptide from chicken albumen with neutral protease
The process of preparing of bioactive peptide from chicken albumen by neutral protease was studied. The factors of affecting process of hydrolysis were investigated. By determining the amino nitrogen content of enzymolysis liquid, it could be concluded that the best conditions for the hydrolysis of chicken albumen were: substrate pH8.0, hydrolyzing temperature 50 ℃, ratio of neutral protease 1.40%, concentration of substrate 70% and total hydrolysis time 5 h. Under these conditions, the amino nitrogen content of enzymolysis liquid could reach 1251.86 mg/kg.