The DiversiLab typing research of Staphyloccocus aureus strains isolated from quick frozen dumpling samples
Objective:To conduct molecular typing research for the 20 Staphyloccocus aureus strains isolated from quick frozen dumpling samples submitted by the same food production enterprise.Methods:DiversiLab automated typing system was used to study the genetics and cluster distribution of the 20 Staphyloccocus aureus strains.Genomic DNA of the 20 Staphyloccocus aureus strains were amplified using the DiversiLab Staphylococcus kit for DNA fingerprinting.The amplified product were analyzed using the DiversiLab system which includes the separation of amplicons on microfluidics chips and webbased data analysis software.Results:According to the diagnostic criteria,the 20 Staphyloccocus aureus strains were distributed to 13 patterns.Among them pattern 1 contained strains L17,L14,L11;Pattern 2 contained strains L16,L12,L19,L4;Pattern 3 contained strains L3,L18,L13.The rest ten strains were distributed to ten different patterns.Conclusions:Based on their genotypes,20 Streptococcus aureus strains show certain degree of aggregation,suggesting they may come from similar sources of contamination.Meanwhile,some other S.aureus strains show stronger genotype differences,indicating they are relatively far from each other genetically.These strains may come from various pathways of contamination.The convenient and efficient DiversiLab automated typing system is a powerful molecular genotyping tool for food production enterprise to monitor bacterial contamination.