Extraction and antioxidant activity of the soluble protein from Lactobacillus rhamnosus broth
In this paper, ammonium sulfate precipitation method was used to obtain the soluble protein from Lactobacillus rhamnosus B10 broth. The different protein components: the peak Ⅰ, peak Ⅱ and peak Ⅲ. It were purified and isolated using dextran gel Sephadex G-100 column. Their scavenging capacity of DPPH·, superoxide radicals (O2-·) and hydroxyl radical (·OH) were analysed. The results showed that the soluble protein, peakⅠ, peak Ⅱ and peak Ⅲ has scavenging effect on DPPH·, and the peakⅠ has more DPPH· scavenging capacity than peakⅡ, Ⅲ, the soluble protein is less than peak Ⅱ, Ⅲ. The O2-· and ·OH scavenging capacity of the peak Ⅰ is the strongest, and it was higher than 5 mmol/L Vc solution. The O2-· and ·OH scavenging capacity of the soluble protein is weak.