The distribution and expression of GPR30 in mice ovary during the estrous cycle
To obtain the distribution and change of GPR30 in the mouse ovary during the estrous cycle,im-munochemistry SP and RT-PCR methods were used respectively to study the expression of GPR30 and GPR30 mRNA in the mouse ovary during different stages of the estrous cycle.As a result,positive staining of GPR30 was widely located in the granular cells,theca interna cells of the follicles at different levels,oocytes,the luteal cells and interstitial cells,while the deepest staining of GPR30 was found in secondary follicles and mature follicles's granular cells.The immunoreactivity positive substance was mainly distributed in the cell membrane and the cy-toplasm.The relative expression levels of GPR30 reached the lowest in the diestrus stage(P0.01);its expres-sional level was the highest in the estrus stage,but there was no significant difference between proestrus,estrus and metestrous(P0.05).The changing regularity of GPR30 mRNA was consistent with GPR30 during the es-trous cycle,its expressional level was significant lower in the diestrus stage than that in other stages(P0.01),and reached its peak level in the estrus stage(P0.01).In conclusion,the results suggest that GPR30 involves in regu-lating the development of follicles and maturity of oocytes during the estrous cycle.