Methylation of the Human Pendrin Promoter
Inspection of the nucleotide sequence of the human pendrin promoter revealed the presence of a CpG island. We investigated the ability of IL-4 to stimulate pendrin message expression in two separate cell lines: the NCI-H292 lung epithelial cell line and the human embryonic kidney (HEK)-Blue cell line. The expression of pendrin mRNA was significantly increased in both cells types after 4, 24, 48 and 72 hours treatment with IL-4, and interestingly, the increase in pendrin mRNA was greater in the NCI-H292 cells. Methylation of CpG sites within the promoter regions of genes can affect activities of gene promoters and have either positive or negative implications on the transcription and mRNA expression of the particular gene. We quantitatively analyzed the methylation status of 35 CpG sites within the human pendrin promoter in both cell lines. The basal methylation pattern was statistically different at multiple CpG sites between the NCI-H292 and HEK-Blue cells. We propose that the difference in basal methylation between the two cell types may determine a cell-specific response to IL-4 in terms of pendrin mRNA expression.