Gene Structure Analysis and Molecular Marker Development of HbTCTP
The translationally controlled tumour protein(TCTP)is a ubiquitous and highly conserved protein in all eukaryotic organisms.As a multi-functioning protein,TCTP carries out vital roles in various life processes.Based on the known cDNA sequence of HbTCTP,we designed the primers to amplify the genome of HbTCTP,and got the genomic sequence of HbTCTP by sequencing the PCR product in this research.The results from aligning genomic and cDNA sequence of HbTCTP showed that HbTCTP contained four introns and five exons,and all intron/exon boundaries were in accordance with the GT/AG rule.The SSR and ILP markers were developed according to the nucleotide sequences and gene structure of HbTCTP,and the polymorphisms of SSR and ILP markers were further detected among 10 Hevea clones.The results showed that the SSR and ILP markers indicated polymorphic among 10 Hevea clones,and the polymorphisms of SSR and ILP markers resulted from the repeat time of SSR and length of intron,respectively.