Isolation, identification and fermentation kinetic of succinic acid producing strain
Succinic acid is a kind of intermediates of tricarboxylic acid cycle, which is widely used in fields of food, chemistry and pharmaceutical industry. A potential succinic acid producing strain named BS2012 is screened from distillers yeast. It was determined that this strain belongs to Bacillus Smithii by the physical and biochemical determination and 16S rDNA molecular analysis. Fermentation kinetics research, which is based on Logistic mathematical model and Luedeking-Piret mathematical model, results in mathematical model and model parameters of strain growth and succinic acid producing. The model calculated results match satisfactorily with the experimental data, which provides a reasonable description of strain growth and succinic acid fermentation process and its dynamic mechanism.