Cloning of BCH Gene and Construction of Two-gene Plant Expression Vector Harboring LCYE RNAi and Antisense BCH in Chinese Narcissus(Narcissus tazetta var. chinensis)
A pair of specific primers were designed and synthesized according to the reported cDNA sequence of the BCH gene in Narcissus pseudonarcissus. An ORF of 959 bp fragment of BCH in Chinese narcissus(Narcissus tazetta Var. chinensis) was isolated, encoding 303 amino acid. With LCYE RNAi and antisense BCH as the targeted genes, a dual plant expression vector was constructed using vectors of pKANNBIAL, pBI121 and pCAMBIA1301. The work will lay the foundation for the genetic breeding of Narcissus tazetta Var. chinensis.