Characterization of a Broad-spectrum Neutralization Monoclonal Antibody against Haemagglutinin of H5 Subtype Avian Influenza Virus
The monoclonal antibody (McAb) 8H5 against the haemagglutinin of the H5 subtype avian influenza virus (AIV) was developed by fusing SP2/0 myeloma cells with the spleen cells of BALB/c mice immunized with Ck/HK/Yu22/02(H5N1). McAb 8H5 was nonreactive to H5-HA by indirect immune fluorescence test and hemagglutinin inhibition test (HI) with 13 strains of non-H5 standard AIV. McAb 8H5 showed high reacitivity with 33 strains of H5N1 virus isolated from 2002 to 2006 in different areas and hosts by HI test and neutralization test (NT). These results suggested that McAb 8H5 is a special broad-spectrum neutralization monoclonal antibody against haemagglutinin of H5 subtype AIV and recognizes a very conserved epitope in HA. Based on McAb 8H5, a diagnostic assay for H5N1 viruses was developed and the results suggested that McAb 8H5 will be of great use in diagnosing H5N1 viruses.