Identification of a New Anthracnose of Camellia oleifera Based on Multiple-gene Phylogeny
Camellia oleifera anthracnose is one of the most important plant diseases in China. In order to further sdudy the anthracnose, samples of diseased leaves were collected form Fenyi, Jiangxi and Chengmai, Hainan.Three strians different from other strains which could infect the leaves were obotained. Their colonies on PDA media were round with color from creamy to aurantiacus, and the mycelium growing speed was 10.8-11.3 mm/d;The appressorium from the mycelia was round, puce to sepia, neat edges,(5.3±0.9)μm×(16.1±1.1)μm sized; The conidial spores were pink first then appeared to black spore balls,(14.5 ±2.5)μm×(5.6±1.6)μm sized. The multigene phylogenetic tree system showed that the 3 pathogenic strains and the typestrain of Colletotrichum boninense clustered into one clade, the confidence level was 100%. Utilizing morphology and multi-gene analysis to indentify the pathogens, the results indicated that they belonged to C. boninense and they were a new recorded anthrax pathogen which could infect C. oleifera.