Determination of affinities and antigenic epitopes of recombinant human tumor necrosis factor-α with monoclonal antibodies by biosensor
Objective To determine the affinities of 10 monoclonal antibodies specific for recombinant human tumor necrosis factor α and 5 monoclonal antibodies recognizing antigenic epitopes of TNF α. Methods By using biosensor the affinities were measured by means of kinetic analysis, and the antigenic epitopes were determined subsequently. Results It was shown that the affinity constants of the antibodies ranged from 10 5 to 10 7M 1 and the antigenic epitopes could be categorized into 3 groups. Conclusion The biosensor is a ideal and powerful tool to facilitate the kinetic analysis of interaction between antigen and antibody and for prediction of conformational distribution of antigenic epitopes.