Co-occlusion of Baculovirus Enhancin with Polyhedrin
Granulovirus enhancin is a group of proteins capable of increasing the infectivity of nucleopolyhedrovirus.A polyhedrin gene negative recombinant vAcBBH-TnEn expressing Trichoplusia ni granulovirus enhancin was constructed,and used to infect SF21 cells jointly with wild type AcMNPV.In the course of infection,and the polyhedra of wild type AcMNPV could be co-occluded into the recombinant virus.SDS-PAGE,immunoblot and immunofluorescence assay demonstrated that enhancin and polyhedrin were expressed simultaneously,the enhancin expression and polyhedra formation occurred in most of the jointly infected cells.Analysis of purified polyhedra from jointly infected cells showed that recombinant enhancin bound to the polyhedra.Enhancin binding to the polyhedra can effectively improve the infectivity of the nucleopolyhedrovirus.