Determination of the major amino acid components by high performance liquid chromatography coupled with fluorescence detection during the development of strawberry fruits.
Amino acid,as one of important compositions influencing fruit quality,takes a wide participation in a lot of physiological metabolisms in fruit trees.The qualitative and quantitative analysis on the amino acids in fruit trees and fruits,provide the research clue for illustrating the complicated mechanism of carbon-nitrogen metabolism and evaluating fruit quality accurately.In this paper,high performance liquid chromatography coupled with fluorescence detection was applied for the analysis on the major amino acids during the development of strawberry "Fengxiang" fruits.The results showed that the components of amino acids in strawberry fruits can be well separated with the eluted gradient of acetate-phosphate buffer(A phase),acetonitrile(B phase)and water(C phase).The method showed perfect accuracy,stability,reproducibility and good sample recovery on the basis of the methodological analysis.The quantitative analysis suggested the major amino acids were serine,arginine,glutamic acid,histidine,aspartic acid and proline in strawberry fruits.During the development of strawberry fruits,the total content of amino acids decreased gradually,from 48.39 μg·g-1 in immature stage to 8.62 μg·g-1 in mature stage.The major components of amino acids with higher contents differed significantly such as serine,arginine and histidine,and the amino acids with lower contents often gave small decrease.The comparing results in this experiment provided the experimental foundation for the qualitative and quantitative analysis on the amino acids in fruit trees and other biological samples.