Extraction of Epimedium polysaccharides by microwave-assisted technology and the antibacterial activity
The microwave assisted extraction of polysaccharides from epimedium was studied.The orthogonal test was adopted to determine the optimum extraction technology,based on the result of single factor influence.The best technical parameters were as followed: the proportion of material to liquid was 1:50,the extracting time of microwave was 10 min,microwave power was 400 W,under the best extracting conditions,and the recovery of the epimedium polysaccharide was 10.14%.At the same time,the antibacterial activity of epimedium polysaccharide was studied.The results showed that epimedium polysaccharide had inhibitory effects on Bacillus subtilis,Escherichia coli and Staphylococcus aureus.The minimal inhibitory concentration of epimedium polysaccharide to Bacillus subtilis,Escherichia coli and Staphylococcus aureus were 1.6 mg/mL,0.8 mg/mL and 3.2 mg/mL.Simultaneity,epimedium polysaccharide had inhibitory effects on Fusarium oxysporum f.sp.niveum,Rice "bakanae"Gibberella fujikuroi Wollenweber and Aspergillus niger,but had no inhibitory effects on Bacillus thuringiensis,Apple Monilla leaf blight Monillina mali Whetzel,Colletotrichum lindemuthianum and Trichoderma.