Studies on Interaction of Bilirubin,Copper ions and 2,2'-Bipyridyl by Spectral Methods
The interaction between bilirubin and copper ions was studied by fluorescence method using 2,2'-bipyridyl as a hydroxyl radical trapper through the hydroxylation of the aromatic compound.The mechanism of bilirubin binding with copper ions and the hydroxylation reaction was studied.It was found that bilirubin was able to bind to copper ions and produced hydroxyl radical surely.2,2'-bipyridyl,an almost non-fluorescence compound was transformed into a highly fluorescence substance on trapping hydroxyl radical,leading to a strong fluorescence increase.When the scavenging agent of hydroxyl radical was added to the system,the enhancement of fluorescence intensity of the system decreased dramatically.The effects of reaction time,pH,and concentrations of bilirubin,copper ions and 2,2'-bipyridyl on the fluorescence intensity were studied.