Simultaneous Determination of Nine Phenolic Metabolites of Polycyclic Aromatic Hydrocarbons in Human Urine by Liquid Chromatography Tandem Mass Spectrometry
A method was developed for the simultaneous determination of nine phenolic metabolites of polycyclic aromatic hydrocarbons(OH-PAHs) in human urine,i.e.2-hydroxynaphthalene,1-hydroxynaphthalene,2-hydroxyfluorene,3-hydroxyphenanthrene,1-hydroxypyrene,3-hydroxychrysene,6-hydroxychrysene,1-hydroxybenz[a] anthracen,9-hydroxybenzo[a] pyrene by solid-phase extraction-liquid chromatography tandem mass spectrometry(LC-MS/MS).After adding the internal standard(13C3-Hydroxyphenanthrene),the urine samples were hydrolyzed by enzyme(pH 5.0) at 37 ℃ for 4 h,then cleaned up and concentrated on C18 SPE column,and then the targeted compounds were analyzed by LC-MS/MS under negative ion mode using Waters symmetry C18 column as analyzed column.The mobile phase was CH3CN ∶ NH3·H2O(0.2%)=72 ∶ 27(volume ratio).The spray voltage was 4 KV.The capillary temperature was 300 ℃.The linear ranges were 0.90-100 μg/L.The correlation coefficients were 0.9970-0.9990.The detection limits were 0.04-0.90 μg/L.The recoveries were 79.0%-119.8%.The relative standard deviations were 4.3%-12.5%(n=6).The method has been used for the simultaneous determination of nine phenolic metabolites of polycyclic aromatic hydrocarbons(OH-PAHs) in human urine.