Comparison of in vitro antioxidant activity of infusion, extract and fractions of Indonesian cinnamon (Cinnamomum burmannii) bark.
The purpose of this research was to compare antioxidant activity of Indonesian cinnamon bark infusion, extract, its fractions and to analyse of their phytochemical constituents for antioxidant activity. The cinnamon infusion was obtained by water extraction, while the extract was by ethanol percolation. The ethanolic extract was then fractionated into n-hexane, ethyl acetate and water fractions.Their in vitro antioxidant activity was assayed semiquantitatively by using DPPH method, while the phytochemical constituents were analyzed by using TLC-autography with several spray reagents.The results showed that antioxidant activity of infusion, extract and its fractions were significantly different. Among the material tested, the cinnamon bark infusion had the highest antioxidant activity, followed by ethanolic extract, its waterand ethyl acetatefractions with IC50 value of 3.03; 8.36; 8.89; and 13.51μg/mL, respectively. Their antioxidant activities were higher than rutin, with IC50 of 15.27 μg/mL.The phytochemical analysis results indicated that polyphenol (tannin, flavonoids) and phenolic volatile oil are the major antioxidant compounds.