Investigation of the structure and function of the “high pathogenicity island” of Yersinia enterocolitica WA in enteroaggregative E.coli strains
Objective To detect the “high pathogenicity island”(HPI) of Yersinia enterocolitica(Yen) WA in enteroaggregative E.coli, as the theoretical base of bacterial evolution process and the different structure of E.coli. Methods Polymease chain reaction(PCR), nucleic acid hybridization in situ, DNA sequencing and SDS-PAGE. Results Five strains were isolated from 6 EAggEC strains, in these isolates, the HPI bordered by an asntRNA locus, as in Yersinia spp, at the condition of iron deficiency, EAggEC expressed two high molecular weight proteins, HMWP1 and HMWP2. Conclusion The HPI of Yen was found in EAggEC, and the HPI which habored the asntRNA locus has full structure and same iron uptake function as Yersinia, maybe the HPI of EAggEC and Yen shares a same mechanism of transfer.