Preparative High Performance Liquid Chromatography-based Multidimensional Chromatography and Its Application in Traditional Chinese Medicine
Abstract Separation of active components from Traditional Chinese medicine (TCM) is always difficult due to its complex matrix. The prep-HPLC-based multidimensional chromatography, which combined the characteristics of different separation techniques to improve separation capability and efficiency, is more conductive for separating and purifying complex TCMs. In this paper, we reviewed the basic principles, separation mode, key techniques of prep-HPLC-based multidimensional chromatography and its application in TCM research.