Cloning and Sequence Analysis of Squalene Synthase Gene in Eriobotrya japonica L.
The cDNA of SQS(Squalene Synthase Gene), which was an importent enzyme of terpenes synthesis,was cloned with RT-PCR and RACE from cell suspension culture of Eriobotrya japonica L. The full length of EjSQS cDNA(GenBank, JQ294055), about 1 775 bp, consisted of an open reading frame of 1 239 bp, and 5′ and 3′un-translated regions of 97 bp and 439 bp respectively. The putative protein had 412 amino acids, and the identity to the other polypeptides varied between 79% ~94%, contained Trans_IPPS_HH conserved domains, and belonged to isoprenoid biosynthesis enzymes, class 1 family. It was the foundation of the further regulation of loquat cell suspension cultures to extract the desired product, using genetic engineering.