Study on gene engineering inulinase hydrolyzed hypso-concentration sucrose
The recombinant inulinase strain GS115/INU1 was induced and expressed. After broth separation and purification, enzyme liquid of GS115/INU1 was obtained which presented single zone in SDS electrophoresis. The optimum temperature and pH of the sucrose hydrolysis were at 50℃ and pH4.0,respectively.Under 48U enzyme per gram sucrose condition, the enzyme hydrolyzed sucrose solution at concentration 50% for 6 hours, and the hydrolysis rate approached 99.7%. The enzyme hydrolyzed sucrose solution at concentration 80% for 14 hours , the hydrolysis rate approached 97.8%.