A novel spliced form of meyoloid progenitor inhibitory factor-1
Objective To study the structure and function of MPIF-1 (meyoloid progenitor inhibitory factor-1). Mehtods RT-PCR, DNA sequencing, computer comparision, DNA recombination and prokaryotic expression, ionexchange chromatography. Results There was a novel spliced isoform MPIF-1β. DNA sequencing showed additional 17 amino acids encoded by the third exon, and replaced the 5′R with a G. SDS-PAGE showed that MPIF-1β recombinant protein has a molecular mass about 14kD-15kD. Bioassay showed that the MPIF-1β can also inhibite haemopoietic stem cell proliferation. Conclusion The MPIF-1β is a novel isoform of MPIF-1 with similar bioactivity. [