Preliminary Studies of Prokaryotic Expression of Hepatitis G Virus Gene
The prokaryotic expression vector pRSET of (and) pGEX was used to express overlapping clones emcompassing hepatitis G virus Core-NS3 and NS5 in E. coli. CE1、E2、NS3、NS5 and chimeric NS3-NS5 gene could produce recombinant proteins efficiently. The ratio of every recombinant protein to total bacteria proteins varied from 10% to 35%. All recombinant proteins were immuoscreened, the results showed that seven of them were immuoreactive. To some extent,we identified the epitopic distribution of recombinant HGV antigens and made a solid foundation for the research of HGV serology and immunological diagnostic reagents.