The SNPs of chemokine RANTES promoter and the mutation of its' receptor CCR5 in SLE and controls in Han indigenous in Chinese
Objective To evaluate frequency and polymorphism of chemokine RANTES and its′ receptor CCR5, and their effects on SLE in indigenous Han Chinese. Methods 146 defined SLE patients and 159 controls were collected. SNPs of RANTES promoter and polymorphism of CCR5 were performed by PCR/RFLP and confirmed by direct DNA sequencing. Results The genotyping frequencies of RANTES-403 G/G, G/A and A/A in SLE were 76.71%, 21.92% and 1.37%, respectively; while the genotyping frequencies of RANTES-403 G/G, G/A and A/A in control were 67.30%, 29.56% and 3.14%, respectively ( P 0.05). There is no significantly difference in the frequency of mutation -403A allele between SLE and controls (12.3% vs 17.9%, P 0.05 ). The genotyping frequencies of RANTES-28 C/C, C/G and G/G in SLE were 76.71%, 21.92% and 1.37% , respectively, which were not significantly different from those in controls as 67.30%, 29.56% and 3.14% ( P 0.05 ). There is no significantly difference in the frequency of mutation -28G allele between SLE and controls ( 12.3% vs 17.9%). The frequency of mutation CCR5△32 was 0 in SLE and 0.3% in controls, which was not significantly different ( P 0.05). The frequency of mutation -403A allele in indigenous Han Chinese was significantly lower than that in North American Blacks and West African Blacks ( P 0.05). The frequency of mutation -28G allele in indigenous Han Chinese was different from that of North American Caucasians, North American Blacks, West African Blacks and North American Hispanics, respectively ( P 0.05). Conclusions These results indicate that the SNPs of RANTES promoter and mutation of CCR5△32 are not related to SLE. There are differences of the SNPs of RANTES promoter and the mutation of CCR5△32 in different ethnic populations.