Building the Counterstain Technique for FISH to Enumerate Soil Bacteria
Fluorescence in situ hybridization(FISH) is widely used to describe bacterial community in marine,lake and river sediments.However,the application of FISH to soil or compopst still need to improve.In this study,the contrast stain for FISH was selected,and the dyeing conditions were analyzed.The results show that 1/200 dilution of SYBR Green I(SGI) is useful as the counterstain for FISH.The dyeing time is 1 min,and non fluorescent immersion oil was recommended to mount.