A Novel Molecularly Imprinted Fluorescence Test Strip for Detection of Cimaterol
Abstract A novel test strip for rapid detection of cimaterol was prepared by combining highly specific selectivity of molecularly imprinted technology with convenient rapid response performance of test strip. The cimaterol molecularly imprinted polymers (MIPs) were prepared through chemical polymerization method, and nitrocellulose membrane was immersed into MIPs clouding solution. After that, the template molecules were eluted and then a clipping step was conducted for obtaining the test strip. After re-adsorption of cimaterol, fluorescent dye Eosin Y was added onto the surface reaction zone on the strip. Qualitative and quantitative analysis would be realized via determining fluorescence quenching in its reaction zone. The experimental results showed that the fluorescence quenching had a good relationship with cimaterol concentration ranging from 0.01–100 μg mL −1 with the limit of detection of 0.01 μg mL −1 . The molecular imprinting test strips provided convenient way in practical application especially for the detection of pork and fodder sample on site.