Impact of altered CIIpeptide on T cell response in collagen-induced arthritis
Objective To explore the mechanism of the inhibitory effect of CⅡ268-270 altered peptide on collagen-induced arthritis. To study its effect on TH1/TH2 balance, anti-CⅡIgG antibody isotypes and regulatory T cells. Methods The altered peptide was given intravenously with a dose of 90μg once a week. Peptide control and blank control were treated with similar approach. The serum TH1/TH2 cytokines were measured by ELISA. The anti-CⅡantibody and its isotypes were measured with ELISA. The induction of regulatory T cells were assayed by real-time RT-PCR analysis of its marker Foxp3. Results The serum IFN-γin rats treated with altered peptide, control peptide and blank control were 41.42±7.32, 54.63±11.15 and 57.86±5.64 pg/ml respectively(P0.01). The serum IL-10 in rats treated with altered peptide, control peptide and blank control were 40.18±5.13, 31.09±5.04 and 30.19±5.02 pg/ml respectively (P0.01). The Foxp3 mRNA abundance in spleen mononuclear cells of rats treated with altered peptide, control peptide and blank control were 33.62±12.12, 10.05±6.08 and 9.73±6.03 respectively(P0.01). Conclusion CⅡ268-270 altered peptide could regulate the balance of TH1/TH2 cytokines. It could decrease anti-CⅡIgG2a antibody. It could also up-reg-ulate Foxp3 + T cells in vivo.