Study on selective differential medium of Vibrio hollisae
Objective: To develop a more economical,simple and rapid separation,identification or detection method of Vibrio hollisae.Methods: We designed and developed the selective differential medium according to the nutritional requirements,the specific enzyme systems,the corresponding metabolisms and the tolerance to different antibacterial ingredients of Vibrio hollisae.We did the tests of color effects,plating efficiency,sensitivity and specificity to evaluate the efficiency of Vibrio hollisae selective differential medium(VhDM).Results: We used VhDM to detect Vibrio hollisae and got good selectivity and specificity.The Vibrio hollisae grown to be red colonies on VhDM after incubation for 16~24 h at 37 ℃,while other species of bacteria no growth or formed yellow colonies.The plating efficiency of Vibrio hollisae on VhDM was(90.65±1.72)% and its detection limit was 10 cfu/mL.Conclusion: As compared with the conventional methods,the method of rapid separation and detection of Vibrio hollisae with VdDM is simpler operation,lower cost,more convenient for observation,higher sensitivity and specificity.Accordingly,it is suitable for kinds of inspection.