Extraction of genomic DNA and optimization of RAPD technique system in melon
The changes of extraction method by CTAB have influence upon the quality and yield of the genomic DNA in melon.The orthogonal experiment L9(33) was made to investigate efficiency of the RAPD-PCR in different conditions,the optimal combination of ingredient in RAPD-PCR was established.The result of repeated experiment showed that the established system was stable and reliable.