Mutation breeding strains and optimization of solid state fermentation medium for AMP deaminase production by Aspergillus melleus
In order to further enhance the activity of AMP deaminase produced by Aspergillus melleus, We used ultraviolet mutagenesis to screening strains, and get a mutant strain that the activity improved by 40%. Then the solid state fermentation medium of the mutant strain was optimized by single factor and orthogonal design methods. The results showed that the best fermentation medium composition was glucose 4%, corn steep powder 1%, Na2HPO4 0.3%, KH2PO4 0.2%, MgSO4 0.1%, wheat bran 20 g and water 20 g. Under the above optimum fermentation conditions the enzyme activity of AMP deaminase can be enhanced to 7533 U/g, increasing 49.27% than before optimization.