Quantificative Determination of 8 Primary Metabolites in Huangqi Injection by ~1H NMR with Deconvolution
To establish the quantification method for 8 primary metabolites( amino acids,organic acids and carbohydrates) in Huangqi Injection,proton magnetic resonance(1H NMR) technique was employed based on processing approach of global spectral deconvolution( GSD). About 10 mg of each sample was precisely weighed after freeze-drying,and then directly dissolved in D2 O for NMR analysis. 20 primary metabolites and4 secondary metabolites were detected and identified in the same1 H NMR spectrum. Among them,8 primary metabolites( acetate,proline,pyoglutamate,malonate,glycine,glucose,sucrose,formate) were selected to determine the contents using an internal standard method. In the given concentration ranges,it showed a good linear correlation for 8 components( R20. 9990),with the RSD of precision lower than 3. 8%,the RSD of repeatability lower than 2. 7%,the RSD of stability lower than 4. 5%,and the recovery rate between91. 40%-102. 7%. The results indicated more accurately integral areas could be obtained by GSD processing compared to the ordinary method,which further improved the accuracy of content analysis.1H NMR method based on GSD was successfully used to determine the levels of 8 primary metabolites in Huangqi Injection samples of 20 batches from 5 manufacturers. The score plots of PCA also showed the samples from different manufacturers clustered and classified well.