Determination of Proteins in Biology Samples by Synchronous Fluorescence Technique with JDC-108 as Fluorescence Probe
JDC-108(chemical stucture see Fig.1) can react with serum albumin to form a complex.Based on this,a new method for the determination of proteins by synchronous fluorescence spectrometry under simulated physiological conditions was established using JDC-108 as a fluorescence probe.The spectral characterization and intensity of synchronous fluorescence were related to the value of Δλ,reaction medium and reaction temperature,and so on.The synchronous fluorescence intensity of the system was in proportion to the concentration of protein in the range of 2.0-497 mg/L for HSA when T=20 ℃,Δλ=20 nm,Tris-HCl buffer(pH 7.40) and ionic strength was 0.1 mol/L of NaCl solution.The detection limit was 0.76 mg/L(n=11) for HSA.Based on these it had been applied to the determination of proteins in serum,urine and saliva samples with satisfactory results,the recovery was in the range of 97.7%-103.4%.The results suggested that the method possessed easy of implementation,rapidity,high sensitivity,broad linear range,better precision and recovery.The method was employed to determine the total proteins in biology samples with satisfactory result.