Research Progress on Plant Chloroplast Proteomics
Subcellular proteomics is a hotspot in the study of proteomics in recent years,and proteomic analysis is a powerful approach to identify plant functional proteins.As an important plant organelle,chloroplast has been intensively studied in plant proteomics.With the sensitivity improvement of two-dimensional electrophoresis and mass spectrometry,and combining with the in-creasing database information from Arabidopsis,rice,corn and other plants,many chloroplast proteins can be quickly identified.Compared with other subcellular proteomics,chloroplast proteome is more comprehensive.Sub-cell separation system,together with two-dimensional electrophoresis that identify the protein components of chloroplast proteins is a useful method to obtain information of chloroplast proteins and to identify the function.This paper mainly describes the development of proteomics and proteomics technologies,and gives a review of chloroplast proteins in order to fully understand the composition and character of chloroplast proteins,and their role in chloroplast metabolic network.