Quantitative Determination of Sequence-specific Nucleic Acid Based on Fluorescent Labeled DNA Probe and Dye SYBR Green I
A synchronous fluorescence spectral method was developed for the quantitative detection of sequence-specific DNA.Graphene oxide was used to quench the fluorescence of the dyes.When the target DNA was added,the fluorescences of the two dyes were recovered due to the formed DNA duplex was far away from graphene oxide.Under the optimum conditions,the linear range was 1×10-10-2×10-9 mol/L,the calibration curve was y=63.388x+9.3862,R2=0.9954.The detection limit of 85 pmol/L was estimated with 3σ(where σ was the relative standard deviation of a blank solution,n=11).This method has good sensitivity,selectivity and accuracy.