Culture of mouse adrenal cortex Y-1 cell line on microcarriers in cell reactor.: Growth and steroidogenesis
Mouse adrenal cortex Y-1 cell line was cultured on microcarriers in cell reactors containing 250 ml or 1500 ml culture medium; the same cell density as in the smaller vessel (0.9 × 106 cells ml−1) was obtained in the larger reactor by controlling pH and supplying the medium with O2. Consumption of glucose, oxygen, production of lactate and variation of 19 amino acids were determined with and without oxygen supply. Synthetic ACTH 1–24 induced steroidogenesis in a serum poor medium supplemented with cholesterol and albumin. Steroid production was similar to that of cell growth in usual culture dishes (2 μg 106 cells−1 24 h−1).