Optimization of the paeonol extraction process from cortex moutan
Two different extraction methods, steam distillation and supercritical fluid extraction, were compared. Using the extraction rate as an index, conditions for paeonol extraction from cortex moutan were optimized using the orthogonal test L9(34). The antibacterial activity of paeonol was further studied. Effects of various factors on the paeonol extraction yield were observed through the response surfaces and contour lines. As a result, the supercritical fluid extraction was better than the steam distillation method. The optimal conditions for paeonol extraction from cortex moutan were: extraction at 50℃ under the pressure of 23 Mpa, with the CO2 flux of 16 L/h, and separation at 40℃. The extraction rate was 1.95% and the extracted paeonol showed the significant antibacterial activity. In conclusion, the supercritical fluid extraction method is of simple operation, high extraction efficiency and suitable for industrial mass production.