Determination of 10 Mycotoxins in Chinese Herbs and Chinese Medicines by Liquid Chromatography-Tandem Mass Spectrometry Using On-line Immunoaffinity Purifying
A reusable on-line immunoaffinity purification column was prepared through packing multitoxin immunoaffinity column filler in stainless column.The loading solution of on-line immunoaffinity purification column was phosphate buffered saline(PBS,pH 7.4),the washing solution was water,and the transfering solution was(1∶1,V/V) acetonitrile-water.The mycotoxins in Chinese herbs and Chinese medicines were extracted by 75% methanol-PBS.The extract was directly injected into the on-line immunoaffinity column which was coupled with reversed-phase analysis column.After high selective purification of the on-line immunoaffinity column,the mycotoxins were eluted onto analysis column for final separation and analysis.Ten mycotoxins: Toxin T2(T-2),Toxin HT 2(HT-2),fumonisin B1,fumonisin B2,ochratoxin A,zearalenone,α-zearalanol,β-zearalanol,α-zearalenol and β-zearalenol,could be detected by the method.The detection limits of the 10 mycotoxins were 1-5 μg/kg.The average recoveries of three levels spiking were 62.3%-107.1%,and the relative standard deviation were ranged from 2.1% to 15.0%.