Cloning and identification of differential genomic genes of antibiotic resistant Neisseria gonorrhoeae by suppression subtractive hybridization
Objective To clone the differential genes in antibiotic resistence Neisseria gonorrhoeae . Methods Using suppression subtractive hybridization construct, to construct the library which contains the differential genes between antibiotic resistance strain and standard reference strain of Neisseria gonorrhoeae . Then the antibiotic resistance relational genes were cloned and analyzed. Results Antibiotic resistance Neisseria gonorrhoeae subtractive library which has high subtractive efficiency was successfully set up. The amplified library contained 2500 positive clones. Sequence analysis was performed for 5 clones. All the five sequences were unknown previously. Conclusions The highly efficient DNA subtractive library may establish a solid foundation for screening and cloning specific antibiotic resistance genes of Neisseria gonorrhoeae . The novel differential genes may provide an important clue for studying the mechanism of occurrence and development of antibiotic resistance Neisseria gonorrhoeae . [