Optimization of microwave-assisted extraction of polysaccharides from bamboo leaves by response surface analysis
The extraction of polysaccharides from Neosinocalamus affinins was studied in this paper.Ac-cording to the single factor experiment,three levels of the factors(extraction temperature,time and the ratio of liquid-to-solid) were selected for the Box-Behnken factorial design to establish a quadric regression equation for describing the yield of polysaccharides.By response surface analysis,the optimal extraction conditions were obtained as follows: extraction power of 600 W,extraction temperature of 124℃,extraction time of 44 min and liquid-to-solid ratio of 41:1,under which the experimental yield(0.45%) of polysaccharides accounts for 98.68% of the theoretically predicted value of 0.456% with one-time extraction.