Responsive Characteristics of IL-7 and TGF-β1 in thymus during Exercise-induced Immunosuppression
To investigate the regulatory mechanism of thymocyte development during exercise-induced immuno-suppression,IL-7 and TGF-β1 as well as their mRNA of rats in thymus were tested.A total of 128 Sprague–Dawle male rats,aged 8 weeks,were divided into Control Group and Exercise Group.Incremental ergomitry Exercise pro-tocol(10 m?min-1 in week1,20 min week2,5 m?min-1 increased per week,successive 6 weeks,6 d per week,30 min per day).Samples were taken in week 0,week 2,week 4 and week 6 before exercise,just after exercise and 3 h after exercise,respectively.IL-7 and TGF-β1 as well as their mRNA of rats in thymus were calculated by ELISA and FQ-RT-PCR.Results:1) During six weeks incremental exercise: The responsive tendency of IL-7 and its mRNA presented the shape of "V" letter during week 0 and week2,while there was no distinguished changes during week4,and last decreased in week6.TGF-β1 and its mRNA presented that of inversed "V" letter during every week,except its mRNA was no prominent change in week0.Conclusion: The decrease of IL-7 and increase of TGF-β1 during Exercise-induced immunosuppression could be resulted the disorder of thymic microenvironment which effected thymocyte development.