Determination of Sibutramine in Dietic Teas by Dispersive Liquid-Liquid Microextraction-Back Extraction-Solidification of Acceptor Phase-High Performance Liquid Chromatography
A novel method for the determination of sibutramine in dietic teas by dispersive liquid-liquid microextraction-back extraction-solidification of acceptor phase(DLLME-BE-SAP)-high performance liquid chromatography was developed.The optimal experimental conditions were as follows: 400 μL petroleum ether as the organic phase,120 μL methanol as the dispersive solvent,the mixture of 14 μL of 0.2 mol/L HCl and 1 μL methanol as the acceptor phase.The extraction time was 2 min.High enrichment factor(130-fold) of sibutramine was obtained under the optimal experimental conditions.The linear range was 0.6-200 μg/L.The limit of detection(S/N=3) was 0.2 μg/L and the limit of quantitation(S/N=10) was 0.6 μg/L.The recoveries of the spiked samples were from 91.9% to 108.4%,and the inter-day relative standard deviations were lower than 14.0%.