Cloning and Bioinformatics Analysis of Mn-SOD Gene from the Plantlets of a Wild Banana in Qishan Mountain of Fuzhou(Musa spp., AB Group)
The young leaves of in vitro plantlets of a wild banan(Musa spp., AB group)grown in Qishan Mountain of Fuzhou were used as the materials to clone Mn-SOD gene by RT-PCR and RACE technology. The full-length c DNA sequence was 831 bp, containing 137 bp 5′ UTR, 543 bp open reading frame, encoding 181 amino acids, 151 bp 3′ UTR, and 3′-end involved 17 poly(A)tails. The prediction of protein's physical and chemical properties showed it belonged to a basic protein with 20 108.8 u and p I7.92.