Studies on liver protection of compound wurenchun capsules by serum pharmacology
Objective:To establish a serum pharmacology testing method for compound wurenchun capsules(CWC).Methods:Model of CCl4-induced injury of primary culture hepatocyte was established and rat was used as the donor to prepare serum containing CWC.With hepatocyte increment(MTT),alanine aminotransferase(ALT) in cell culture medium and the content of schisandrin in serum as indices,single-factor investigation and multiple-factor orthogonal experiment were carried out,using serum or plasma,and serum containing drug in culture system of different concentration on pharmacological effect and to screen of the preparative method of serum containing drug and its suitable concentration in culture system.Results:Normal rats as the donator of serum,using methanolic extract of CWC,10 times equivalent dose for adults every day,intragastric administration twice a day of 8-hour interval,keeping on 3 days,hemospasia after 2 hours of the last administration was the better preparative method of the serum containing drug.Adding 10% of this serum to culture system could elevate hepatocyte increment and depress ALT in cell culture medium significantly.Conclusion:Serum containing CWC prepared by suitable method possesses an obvious protective effect on injured hepatocyte and serum pharmacology can be applied to evaluated the pharmacological effect of CWC.