Selection of the electrofusion parameter of reconstructed embryo from Bore-Huanghuai white goat somatic cell nuclear transfer
In this study,cells nuclear transfer experiment was carried out using the ear fibroblasts of Bore goat as donor cells and the oocytes of Huanghuai-white goat as recipient cell.The result showed that when the electrofusion parameter was 250V·mm-1 for one DC pulse,the cleavage rate of oocytes with 40 μs electrofusion time,the electrofusion of reconstructed oocytes was significantly higher than that with 10 μs electrofusion time or 20μs electrofusion time(P0.05),but the survival rate of the reconstructed embryos after the electrofusion with 10μs electrofusion time was the highest(P0.05);when the electrofusion parameter was 250V·mm-1 for 10μs electrofusion time,the cleavage rate of reconstructed oocytes with two DC electrofusion pulses was significantly higher than that with one DC pulse,while the livability of post-electrofusion embryos and the rate of obtainable embryo in one DC pulse were both higher than those in two DC pulses(P0.05).It is the conclusion that the!optimal electrofusion parameters for reconstructed oocytes of goat may be 250V·mm-1,10μs and one DC pulse.