Role of phosphatidylinositol 3 kinase (PI3K) in the intracellular signaling transduction pathways mediated by TCR and CD28 of γδT cell
Objective To explore the role of phosphatidylinositol 3 kinase (PI3K) in the intracellular signaling transduction pathways of human peripheral blood γδT cells activated by CD28 with Mycobacterium tuberculosis (M.tb) low molecular peptide antigen. Methods Flow cytometry was used to detect the expressions of CD69 molecule on activated γδT cells and proliferation rates of γδT cells stimulated by anti-CD28 monoclonal antibody and M.tb antigen. Meanwhile, the PI3K inhibitor LY294002 was added to block the activity of PI3K to find the effect on expressions of CD69 molecule and γδT cells proliferation response. Results The expression of CD69 molecule on γδT cells and proliferation response of γδT cells were blocked by addition of LY294002. Conclusion The intracellular signaling pathways delivered by CD28 and M.tb antigen in γδT cells was transducted by PI3K. [