Establishment of Real-time PCR Assay for Quantitative of Plastid ACCase in Beardless Barnyardgrass
In order to study the resistance mechanism of a quizalofop-ethyl-resistance beardless barnyardgrass,a relative quantitative analysis method was established based on real-time PCR technology and β-actin gene as the housekeeping gene. The results showed the specificity of the two sets of primers was very well. The amplification efficiencies of the primers for β-actin and plastid ACCase gene was 103.2% and 104.3,while the correlation coefficients was 0.993 0 and 0.991 2,respectively. The results showed the real-time PCR quantitative method established in the article could be provided as a methodological reference for the study of the expression of plastid ACCase gene and the study of the mechanism of weed resistant to ACCase inhibitors.