Influence of microencapsulated CHO cells translated mIFN-γ on T_H1/T_H2 in tumor burden mice
Objective To study the influence of transplantation of microencapsulated CHO cells translated IFN-γ gene on T H1/T H2 type cytokines, and adjustment of foreign IFN-γ to T H1/T H2 cells balance. The feasibility of microencapsulated genetic cells as tumor-killer was studied. Methods The mIFN-γ gene was translated into CHO cells. After microencapsulated, the CHO cells translated IFN-γ gene were transplanted into tumor burden mice. After 14 days, the level of serum IFN-γ, IL-2, IL-12, IL-4, IL-10 was examined. The tumor size and survival time was studied also. Results The level of serum IFN-γ, IL-2, IL-12 was significantly higher, but the change of IL-4, IL-10 were not significantly. The speed of tumor growth was significantly slowed down. The survival time was elongated. Conclusions The foreign IFN-γ promotes T H1 cells differentiation, T H1/T H2 move to T H1. The microencapsulated genetic enginnering cells may be a new candidate for cancer treatment as an alternative of genetic enginnering drugs. [