Effect of neoagaro-oligosaccharide on activity of salivary amylase
To study the effect of neoagaro-oligosaccharide (NAOS) on activity of salivary amylase, NAOS was produced from agar by agarase, and the effect of NAOS on enzymatic efficiency, temperature tolerance, pH stability, and the synergistic action with the chloride ion which can be used as activator of salivary amylase were investigated. The results showed that the enzyme activity could be increased to 1.21 times with the NAOS concentration of 100 μg/mL. It has better temperature tolerance at 55 ℃ in 2 hour. It also has better pH stability. The enzyme activity can keep around 28 U/mL in the pH range from 6 to 8, but the enzyme activity of the control group dropped 38%. The enzyme activity could be increased to 1.98 times under the effect of synergistic enhancement between NAOS and chloride. NAOS can be used as activator and stabilizer for salivary amylase. It may have certain significance on gastrointestinal health and dyspeptic disease treatment.