Establishment of Extraction Methods and Two-dimensional Electrophoresis Conditions for Proteomic Analysis of Camellia sinensis
In order to develop efficient two-dimensional electrophoresis(2-DE) conditions suitable for tea proteome analysis, the effects of different protein extraction methods and sample loading amount were compared. The results showed that the modified phenol extraction protocol was more suitable for total protein extraction of tea compared with trichloroacetic acid(TCA)/acetone precipitation. And loading 1.5 mg protein sample, a high quality 2-DE image map with more protein spots, clear background and high protein point resolution was obtained. The research provides a foundation for further study of tea proteomics.