Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR
Detection for HCV with FDthermostable reverse transcriptase mediated RTnested PCR$$$$ ZHENG Zuohua, ZHANG Liping, ZHAO Wei, et al. Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433 【 Abstract 】 Objective In order to detect HCV in human blood, a FDthermostable reverse transcriptase mediated RTnested PCR technique was developed. Methods For comparison, we used this method and conventional ELISA to detect the infection of HCV in 108 patients. Results The results showed higher positive rate in FDTRT mediated RTnested PCR. Conclusion The results suggest that it is a more sensitive, effective and specific way for detecting HCV infection. 【 Subject words 】 FDthermostable reverse transcriptase Polymerase chain reaction Hepatitis C virus