Submerged Culture and Intracellular Polysaccharide of Paecilomyces gunnii var.minor
In this paper,PGM01,a strain of Paecilomyces gunnii var.minor Li et al. was tested for mycelial biomass in submerged culture in 5 different media. The result indicated that the best medium was silkworm pupa powder (sugar 30 g/L,silkworm pupa powder 25 g/L, yeast extract 5 g/L at pH=7～8).Isolation and purification of the polysaccharide by different methods showed that there were 3 components in the intracellular polysaccharides of PGM01. The main component was brownish, of common physical and chemical characteristics.The result of a GC chromatographic analysis on the main component after hydrolysis showed that the component consisted of mannose,glucose and galactose,with their molar ratio at 1 5∶1 3∶1.An IR spectroscopic analysis showed that the component had typical absorption peaks of polysaccharides.