Identification and Sequence Analysis of PAL Gene Family in Solanaceae
Phenylalanine amm onialyase(PAL, EC:4. 3. 1. 24)is a critical enzyme in secondary metabolism of plants, which has significant biological relevance and strong ability against bacteria; also, its product provides precursor for plant natural products such as capsaicinoids. In this study, based on the genome database, 27 peptide sequences belonging to phenylalanine ammonialyase gene family were obtained from Lycopersicon esculentum Mill.(syn. Solanum lycopersicum L.), Solanum tuberosum L., Nicotiana benthamiana Domin, Capsicum annuum L., Populus trichocarpa L and Arabidopsis thaliana(L.)Heynh by BLASTP, and analysed by bioinformatics,physico-chemical properties, structural analysis. The analysis showed that tobacco, tomatoes, potatoes and peppers have close genetic relationship, then Arabidopsis thaliana, poplar is further in evolution. Acidic protein is 96.3%.All PAL proteins are located in cytoplasm, hydrophilic, stable, transmembrane, no signal, and 96.3% of the protein has the active site. The anaytical results provide data for the next study of Solanaceae metabolic mechanism.