Variance analysis between TaqMan probe and SYBR Green real-time quantitative PCR for determining the copy number of transgene in transformed plants
To explore the differences between TaqMan probe and SYBR Green real-time quantitative PCR technique for estimating the transgene copy number in a transgenic plant.we used TaqMan probe and SYBR Green real-time quantitative PCR technique to determine the copy number of twelve transgenic rice,and then used the software SAS 9.1 to analyze the results with the two methods.As a result,the t-test analysis showed that when the amplification efficiency of the endogenous reference gene was close to the target gene,the variances between TaqMan probe and SYBR Green real-time quantitative PCR technique were not significant;As the amplification efficiency of the endogenous reference genes was in accord with the target gene,the results of the two methods were similar.