Study on the kinetics of limited hydrolysis on rice residue with Alcalase 2.4L FG
This paper has investigated the kinetics of limited hydrolysis on rice residue with Alcalase 2.4L FG by pH-stat method.The factors including reaction temperature,enzyme concentration,substrate concentration,pH value and reaction time were discussed and figged out for the reaction.Discussed the kinetics characteristics of the reaction catalyzed by the enzyme.The critical substrate concentration was worked out theoretically as 22.25g residue per 120mL water.The hydrolysis kinetics model was established and proved accurate.The RSD is within 10% in test experiments.