Protection effect of the convalescent serum from HFRS patient on the HTNV infected neurons in vitro
Objective Convalescent serum from patient of hemorrhagic fever with renal syndrome (CSPH) was added to the HTNV infected embryo mouse brain neurons to test the protective action of CSPH on HTNV. Methods Morphological changes were observed with Phase contrast microscopy and the viability of neuron cells were examined with MTT colorimetric assay. Results Significant difference of the A value formd between the HTNV infected neuron cell cultures and uninfected normal control cultures ( P 0.001). While no obvious differences were observed among different serum dose groups both in the convalescent serum protection group and in the negative serum control group ( P 0.05), significant differences of the A value were formed between the convalescent serum groups and the negative serum groups ( P 0.001). Conclusions Convalescent serum from patient with HFRS may produce protective action to the mouse brain neuron culture cells infected with HTNV.