Distribution and genotypic profiles of oral Candida albicans in the elderly
Objective To investigate the distribution and genotypic profiles of C.albicans isolates from the elderly in Chengdu, and then to compare the relativity of the frequency of C.albicans incidence with genotype.Methods A total of 212 healthy individuals(age, 60 years) were divided into four groups: A1(48 men with dentures), A2(61 men without dentures), B1(53 women with dentures) and B2 (50 women without dentures). CHROMagar Candida~TM, Sugar assimilation patterns(API 20C AUX tests) and RAPD(randomly amplified polymorphic DNA assays) were used to determine C.albicans. C.albicans were screened for genotypic profiles by using randomly amplified polymorphic DNA assays(RAPD), two different primers(JWFF and NA) were used to develop DNA profiles.Results Eighty-nine C.albican strains were isolated from 212 healthy individuals(42%). The frequency of C.albicans of A1, A2, B1 and B2 is 56.2%, 21.3%, 56.6% and 38%, respectively. The frequency of C.albicans between A1 and A2 was significant different, P0.01. RAPD analyses with random primers JWFF and NA revealed 5 different genotypes (A-E) among C.albicans. There was significant difference in the genotypic constitution between group A1 and A2. The major type in group A1 was A. [WT5”HZ]Conclusion The frequency of C.albicans were elevated in the denture-wearing groups compared to those in the corresponding non-denture-wearing groups. Denture-wearing may contribute to the increasing frequency of some C.albicans isolates with special genotypic profiles in elderly individuals.