Preparation of gold-immunochromatography strip for rapid detection of E.coli O157:H7
Objective:Make gold-immunochromatography strip for Escherichia coli O157:H7 and test the effect of sensitizer.Methods:Use the gold immunochromatography assay(GICA) technology,the Escherichia coli O157:H7 was detected by double antibody sandwich method.Results:This gold-immunochromatography kits can checkout Escherichia coli O157:H7 in 15 minutes.The sensitivity of the test is 1×106 cfu/mL.For different parasitism population,such as Salmonella,Vibrio parahaemolyticus,Campylobacter jejuni,not find cross-reaction.Indicating good specificity of this strip.Conclusion:The gold-immunochromatography strip is test quick,sensitivity high and well,apply to the express test.