Determination of Polysaccharide in Pea Root Mucilage by Precolumn Derivatization and Capillary Gas Chromatography
A novel gas chromatographic method was developed to determine six kinds of monosaccharide units hydrolyzed by trifluoroacetic acid from polysaccharide in pea root cap mucilage.The baseline separation of the monosaccharide units were achieved with a medium polar capillary column OV-17(35 m×0.32 mm,0.33 μm).The total analysis time was 42 min.Retention times were used to identify the individual sugar in the chromatogram and external standard method was used for quantitative analysis.The linear range of the method was 0.0025-2.5 g/L and the correlation coefficient were over 0.9991.Minimum detectable limit at a signal-to-noise of 2:1 was 0.0216-0.1578 mg/L.The recovery of the six kinds of standard mono sugars was 96.7%-107.4%.The results indicated that this method was effective and quick in analyzing the components of sugars in the mucilage collected from plant root tips under adversity stress.