Optimize the Extraction Process of Bergenin in Callus Culture of Ardisia crenata
The effects of different temperature,time and the ratio of solid to solution on the extraction yield of bergenin in Ardisia crenata were investigated based on composite design of quadratic regression.The extraction technique parameters were optimized with response surface methodology,and the results showed that the optimum extraction parameters were the temperature of 80.08 ℃,the time of 49.74 minutes and the ratio of solid to solution of 1 ∶ 10.00.Under those conditions,the concentration of bergenin reached the maximum with 81.34 μg/mL.