Electrochemical Detection of Alkaline Phosphatase Using Ionic Liquid Modified Carbon Nanotubes Electrode
An electrochemical assay of a steady and dynamic detection method of alkaline phosphatase(AP) was investigated.Using a new composite electrode fabricated with carbon nanotubes and ionic liquids(CNTs-ILE),after working on the characteristics of the electrode,and the electrochemical research of the catalytic reaction substrate p-nitrophenyl phosphate(PNPP) and product p-nitrophenol(PNP),continuous dynamic amperometric detection of the enzymatic reaction was achieved.The results indicated that the cyclic voltammetry characteristics of the CNTs-ILE were better than those of glassy carbon electrode,and the CNTs-ILE had good anti-fouling property when detected PNP.When the concentration range of AP was 1 U/L to 1×102 U/L,the current had a good proportional relationship with the concentration of AP.This method could be used to detect AP within 20 min.CNTs-ILE′s good anti-fouling and low production cost properties made this electrode expect to have a further development in detection of AP.This technique can also be used to analyze a specific sample,which was,on basis of the traditional ELISA method,using CNTs-ILE to electrochemically detect the catalytic reaction product of AP.This method can be used to determine the concentrations of antigen through the current peak,and provide theory basis for the future detection of AP marked antigen or antibody.