Preparation and comparative analysis of antioxidant activity of bamboo leaf extracts from different varieties
Phyllostachys heterocycla,Pleioblastus amaru,Bambusa tuldoides,Bambusa chungii,Bambusa textilis,Chimonocalamus Hsueh and Indocalamus tessellates were used to extract bamboo-leaf-flavonoids by the heat reflux method and purified by AB-8 macroporous absorption resin.The extractions were separated and struc-turally identified by high-performance liquid chromatography(HPLC).The bamboo leaf extracts were assayed based on DPPH radical scavenging capacity,hydroxyl radical scavenging capacity,superoxide anion free radical scavenging capacity,and reducing power with TBHQ(tert-butyl hydroquinone) as the positive control.The results showed that the yield of extracts from Pleioblastus amarus was maximum,up to 3.60%-3.70%;the amount of orientin and isoorientin in Chimonocalamus Hsueh was maximum,up to 2.5230%-2.5326%;the amount of vitexin in Pleioblastus amarus was maximum,up to 0.8870%-0.8896%;the amount of isovitexin in Bambusa textilis was maximum,up to 2.2231%-2.2261%.Chimonocalamus Hsueh revealed the strongest scavenging ca-pacity against DPPH,and the IC50 value was 30.50 mg?L-1;Indocalamus tessellates revealed the strongest scav-enging capacity against hydroxyl radical and superoxide anion free radical,and the IC50 values were 339.32 mg?L-1 and 324.87 mg?L-1,respectively.