Immunological activity of a T-hybrid line. II. Mapping H-2 genes for the SRBC-specific suppressor factor, its acceptor, and suppression of the antibody response.
Studies are reported on the nature and action of a suppressor factor with specificity for sheep red cells produced by a mouse T-hybrid cell line. Absorption of the factor with anti-H-2 reagents indicated that it carries determinants coded between the I-J and D loci. The factor was bound well by spleen cells of certain strains (B10, B10.BR), but not others (B10.S); absorption studies with spleen cells of H-2 recombinant strains suggested that a gene contributing to a cellular acceptor site for the factor also mapped in the region between I-J and D. On the other hand, studies of functional suppression of the anti-SRBC response of different strains in vitro indicated that an H-2 gene in the I-A or I-B subregions controlled suppression. Thus, at least two H-2 genes seem to be involved in the suppressive action of this factor.