Reserpine causes differential changes in the mRNA levels of chromogranin B, secretogranin II, carboxypeptidase H, alpha-amidating monooxygenase, the vesicular amine transporter and of synaptin/synaptophysin in rat brain.
Brains of rats treated with a high dose of reserpine were analyzed by in situ hybridization. The mRNA levels of several components of large dense core and small synaptic vesicles were determined. After drug treatment the secretogranin II message was elevated in the parvocellular neurons of the paraventricular nucleus, in the zona incerta, dorsal raphe, locus coeruleus and in the nucleus tractus solitarius. The levels of chromogranin B mRNA were increased in the dorsal raphe and in the substantia nigra compacta. In control animals messages for synaptin/synaptophysin could be found in most of the nuclei investigated, that of the vesicular amine transporter was only detectable in substantia nigra compacta, the dorsal raphe and the locus coeruleus whereas those of carboxypeptidase H and the alpha-amidating monooxygenase could only be determined in the paraventricular nucleus. All these messages were not changed after reserpine. We conclude that the chromogranin B/secretogranin II messages are regulated concomitantly with various neuropeptides. They represent useful general markers to identify stimulated neurons. Our results are consistent with the concept that stimulation of neurons leads to an increased synthesis of secretory peptides and consequently to large dense core vesicles filled with higher quanta of peptides.