Transfer of herbicide resistant gene bar into cultivated wheat variety by backcrossing
A herbicide resistant gene bar was transferred into two cultivated wheat Wanmai 48 and Yangmai 158 by backcrossing.The test of herbicide-resistance,chlorophyll content,glutamine synthetase(GS)activity and phosphinthricin acetyltransferase(PAT)activity indicated that the backcross generations B2F1 showed positive reaction of the bar gene.It suggested that the foreign gene bar had been transferred into and was expressed in two cultivated wheat.Most of agronomic characters of the backcross generations B2F1 were similar to their recurrent parents.The results confirmed that transfering bar gene into other variety by backcrossing is a simple and effective approach to get a new wheat variety resistant to herbicide PPT.