Isolation, Identification and Metabolites Analysis of Four Marine Rhodotorula Strains
Four marine Rhodotorula strains were isolated from leaves, flowers, fruits and soil of mangrove, sea mud hairtails, octopuses and squids in Techengdao, Donghaidao and aquatic product markets in Zhanjiang,Guangdong Province, China. The isolates were identified by the observation of its morphology, culture characteristics and the analysis of 26S rDNA D1/D2 domain sequence. Results showed that, the four strains belonged to Rhodotorula mucilaginosa strain ZJZ1, Rhodotorula mucilaginosa strain ZJD2, Rhodotorula glutinis strain ZJN5 and Rhodotorula sp. ZJH8; the strains could secrete carotenoid, crude polysaccharide, protease,amylase, cellulase, Vitamin B1, Vitamin B2 and Vitamin B6; the content of carotenoid produced by R.mucilaginosa strain ZJD2 was the highest, 155.86 μg/g; the contents of protease and Vitamin B6 produced by R.mucilaginosa strain ZJZ1 were the highest, 188.96 U/mL and 1.14 μg/mL, respectively; the contents of Vitamin B1 and Vitamin B2 produced by R. glutinis strain ZJN5 were the highest, 9.89 μg/mL and 6.65 μg/mL, respectively; the contents of crude polysaccharide, amylase and cellulase produced by Rhodotorula sp. ZJH8 were the highest,14.696 μg/mL, 99.271 U/mL and 1.913 U/mL, respectively.