Simultaneous Detection of Cytomegalovirus,Human Papillomavirus and Chlamydozoa Trachomatis by Multiplex PCR
In order to develop a polymerase chain reaction-based assay for the simultaneous detection of cytomegalovirus(CMV), human papillomavirus(HPV) and chlamydozodtra chomatis (CT) from a single genital swab, we chose three sets of oligonucleotide primers according to CMV,HPV and CT gene sequences reported that amplified products of 370bp, 450bp, and 510bp. The conditions for multiplex PCR were optimized. By this method, 0.01fg of HPV-DNA,CMV-DNA and CT-DNA could be detected and visualized under UV light, but not the gene fragments of human lymphocyte-DNA, E.coli-DNA, U. urealyticum-DNA and C. albicans-DNA.395 clinical samples were tested, the positive rates for HPV, CMV and CT were 19.2%, 14.9% and 5.1% respectively, and besides, in 42 samples, these three agents mixedly infected. The multiplex PCR appears to be a rapid, sensitive and specific method for detecting CMV, HPV and CT simultaneouly from a single genital swab and may be applied to clinical diagnosis and laboratory works.