Ultraviolet spectrophotometric determination of total isoflavones in the stems and leaves of Microcos Paniculata L.
To determine the content of the isoflavones in the stem and leaves of M. Paniculata L. Genistein as the standard, used the ultraviolet spectrophotometric method to determine the content of the isoflavones in the stems and leaves of M. Paniculata L.. The calibration curve was linear (r=0.9993) in the range of 0.0005～0.0045 μg/mL, and the curve was C=0.0039A. The content of stems was about 0.07% and content of leaves was about0.23%. The spectrophotometry is suitable for the determination of isoflavones from the stems and leaves of M. Paniculata L.. This method operated convenientely, the result was stable, and has good repeatability.