Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis
The structure and function of a bacterial trimeric protein disulfide isomerase was investigated by characterising two variants in which key structural regions were deleted. In one case the effect on structure and function was predictable, while in the other we found unintended consequences for both structure and function.