Determination of acrylamide in fried food by RP-HPLC-DAD
Acrylamide in fried food was extracted by methanol under ultrasonic wave.High performance liquid chromatography(HPLC)equippied with diode array detector(DAD) and C18 reversed phase column was employed for detection of acrylamide using methanol∶water(5∶95) as the mobile phase with 0.80 mL/min flow rate at column temperature of 40 ℃ and the injection volume was 10 μL.Qualitative analysis of acrylamide was based on its retention time according with the standard sample and on its ultraviolet spectra surveyed by DAD.Quantitative analysis was carried out at a wavelength of 210 nm and the external standard method was used.The results showed that calibration curve had high linear correlation(r=0.9990) and,the linear correlation range is between 0.01~0.50 μg/mL.The mean recovery was 98.84% with RSD=2.08% and the detection limit was 0.002 μg/g.The method stablished was sensitive,facile and accurate from the confirmation by application to various fried food samples.