The Comparative Study of Active Ingredients from Coreopsis Tinctoria and Commercial Chrysanthemum
In this experiment,the contents of total flavonoids was determined by Al(NO3)3-NaNO2in spectrophotometric colorimetry,and the contents of active ingredients were determined by HPLC analysis.More over,we compared the differences of active ingredients from coreopsis tinctoria and commercial chrysanthemum.Studies showed that the contents of total flavonoids in the coreopsis tinctoria and commercial chrysanthemum were 117.15～153.04 mg/g and 27.05～51.82 mg/g,respectively.Chlorogenic acid,hyperoside,astragalin,quercetin and kaempferide were the common monomer components between coreopsis tinctoria and commercial chrysanthemum,while catechol,vanillic acid,syringic acid and rutin were only detected in the coreopsis tinctoria,and resveratrol was only detected in commercial chrysanthemum.The total flavonoid content in the coreopsis tinctoria was significantly higher than commercial chrysanthemum,phenolic compounds monomer composition was richer and its content was also relatively high.Accordingly,coreopsis tinctoria was a chrysanthemum variety with higher medicinal value,which had better in-depth research and development value.This experimental method could be applied to the quality control of chrysanthemum and its preparation.