Genetic structure of Wuzhishan miniature pig by using microsatellite DNA markers
Genetic structure including diversity,polymorphism information content(PIC),heterozygosity(H) were chosen to study Wuzhishan miniature pig by using 9 microsatellite DNA markers.Microsatellite loci detected have 1～3 allelic genes,the frequence of allelic gene is between 0.1250 and 1.0000,heterozygosity is between 0.0000 and 0.6226,PIC is between 0.0000 and 0.5480,average heterozygosity is 0.3849 and average polymorphism information content is 0.3270.The average heterozygosity and polymorphism information content of three families are 0.2934,0.2075,0.3005 and 0.2227,0.1588,0.2408,respectively.The results indicated that with increasing of generation,genetic variation is reducing.It is suggested that WZSP is a genetically stability breed and satistifies the genetic standard of closed colony animal,and that the inbreeding degree of three lineages of WZSP are all high,and the richness of genetic diversity is lower than that in general commercial pig breeds.And this can provide theoretic foundation for the development of trial animals.