Cloning and sequence analysis of CP gene of Rice stripe virus Anhui isolate.
Rice plants showing stripe disease were collected from Lujiang,Anhui Province.The total RNA was extracted from rice leaves infected with Rice stripe virus.A pair of primers was designed to amplify RNA3 partial fragment of Rice stripe virus Anhui isolate(RSV-AH) by RT-PCR,and then the fragment was cloned and sequenced.Sequence analysis results showed that the fragment was consisted of 1051 nts,including 969 nts of full-length CP gene encoding 322 amino acids.Comparing nucleotide sequence of RSV Anhui isolate CP gene(RSV-AH) with that of other RSV CP genes,the results showed that RSV CP gene of Lujiang,Anhui shared the highest sequence similarity(99.3%) with RSV CP gene of Hongze,Jiangsu.A phylogenetic tree based on alignment of nucleotide sequences of RSV CP genes was constructed.The result indicated that the RSV CP gene of Lujiang,Anhui was most closely related to that of Hongze,Jiangsu as well.