Meiosis of Pollen Mother Cells and Pollen Abortion in Sugarcane officinarum × Erianthus rockii F_1 Hybrids
The cytological smear techniques was used for studing the meiosis and chromosome behavior of pollen mother cells in Sugarcane officinarum × Erianthus rockii F1 hybrids. The results from microscope observation revealed some abnormal behaviors in meiosis as follows: 1 The univalents, bivalents and quadrivalents were observed in diakinesis; 2A few lagging chromosomes appeared in metaphase Ⅰ, anaphase Ⅰ, metaphase Ⅱ and anaphase Ⅱ; 3The back arch shaped spindle in metaphase Ⅱ and the Daughter cell chromosomes in dyad were out of sync separation in anaphase Ⅱ; 4 There were some abnormal cells in tetrad stage, such as traid,micronucleus, different sized tetrad and polyhedron etc; 5 Different cell division phase co-existed in the three anthers in one flower. Another results from DAPI staining method indicated that most microspores came into shrinkage deformation in the uninucleate stage. Ultimately, it can be concluded that the chromosome abnormal behavior and the cell abnormal division in meiosis should be the main reason for the pollen abortion completely of F1 hybrids, and the pollens abortion couble be occurred in the stage of microspore mother cell, tetrads and uninucleate microspore.