Characteristics of Arsenic Metabolites in Human Liver Hepatocytes
Hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry(HPLC-ICP-MS) was applied to the determination of arsenic metabolites in Chang liver cells after arsenic incubation.Two unknown arsenicals were found,one of which was speculated as methylarsonous acid(MMAⅢ),and further characterized using standard addition method using synthesized MMA Ⅲ standard.Quantitative analysis results showed that monomethylarsenicals,dimethylarsenicals and inorganic arsenicals in hepatocytes after arsenic incubation were increasing with the increase of initial incubated arsenic concentration while the methylation rate decreasing with the increase of initial incubated arsenic concentration,which indicated that high concentration of arsenic has the inhibition to the ability of arsenic methylation of Chang liver cells.