Gene sequencing and analysis of the envelope protein of dengue 2 virus isolated in Hainan China
Objective To determine the complete envelope protein gene sequence of dengue 2 virus strain isolated in Hainan China and characterize its phylogenetic specialty. Methods The sequence of the envelope gene of dengue 2 virus strain isolated in Hainan China was determined by RT-PCR and Sanger method. The C6/36 cells and the new-born mice were infected with this virus strain. Results The whole nucleotide sequence of D2V-HN89 envelope gene was 1?464bp. Nucleotide and amino acid homology were 95% and 98% respectively between D2V-HN89 and D2V-NGC. Meanwhile, D2V-HN89 was most close to D2V-Jamaica and D2V-Brazil90. The cytopathogenic effect of D2V-HN89 and D2V-NGC were similar but the neural virulence of D2V-HN89 in suckling mice was much weaker than that of D2V-NGC. Conclusion D2V-HN89 lose 21bp in its envelope gene sequence as compared with D2V-NGC, D2V-HN89 may come from the epidemic area in Jamaica or Brazil. [