Isolation and Primary Expression Analysis of a Full-Length cDNA Clone Encoding Small GTP-Binding Protein Gene CaRab8 in Pepper
One 961bp full-length cDNA clone was isolated from pepper normalized cDNA library,which containing a complete 651 bp open reading frame and encoding a putative protein composed of 217 amino acids.Amino acid sequence deduced by this cDNA clone showed high similarity to NtRAB8-2 protein from tobacco.The full-length cDNA was named CaRab8.The protein encoded by this cDNA clone included conserved domains for guanine nucleotide binding and GTPase activities and five domains specific to Rab family including YYRGA domain.Real time quantitative PCR analysis showed the expression level of CaRab8 was induced by MeJA while was repressed by SA.