Optimization of the Fermentation Conditions of 5L Fermenter for Geobacillus stearothermophilus CHB1
A single-factor method was used to optimize the conditions of Geobacillus stearothermophilus CHB1 such as ventilation volume,speed,temperature,pH and other parameters,and to determine the growth curve of CHB1 in a 5 L fermenter.The best fermentation conditions were: ventilation 6 L/min,speed 180r/min,inoculum 4% and culture temperature 58 ℃.The maximum cell biomass could be achieved by fermentation 21 h.Through the method of auto-fed acetic acid to control the pH of fermentation process,it could achieve a high-density fermentation of CHB1.The cell biology was as high as 6.07×108 cfu/ml.Using fed acid pH control pH8.0,it could achieve the maximum cell biomass up to 6.07×108 cfu/ml.