GREEN SYNTHESIS AND ANALYSIS OF SILVER NANOPARTICLES USING Leucas aspera
1,2,3 Department of Biotechnology, Bapuji Institute of Engineering and Technology, Davangere AbstractThe green synthesis of silver nanoparticles using plant leaf extract (Leucas aspera) for the reduction of aqueous silver ions. Stable silver nanoparticles were formed by treating aqueous solution of silver nitrate (AgNO3) with the plant leaf extract. The formation of dark brown color confirmed the synthesis of silver nanoparticles. UVvisible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. The size and shape of the nanoparticles was characterized by using SEM (Scanning Electron Microscopy). This environmental friendly method provides simple, easy and cost effective faster synthesis of nanoparticles than chemical methods. Further, the antimicrobial activity of synthesized silver nanoparticles showed effective inhibitory activity against pathogens and non pathogenic bacterium strains viz. E. coli, Bacillus cereus, L. monocytogenes, M. luteus,S. aureus, Yersenia. The cytotoxic effect of synthesized silver nanoparticles is studied by using Brine Shrimp Lethality Test (BSLT). KeywordsGreen Synthesis, Silver Nanoparticles, Plant Leaf Extract, SEM, Antimicrobial Activity, Brine Shrimp Lethality Test