An Enzyme-linked Immunosorbent Assay for Detection of Fenoxaprop-p-ethyl
To develop a competitive indirect enzyme-linked immunosorbent assay(ci-ELISA) for fenoxaprop-p-ethyl,a hapten(fenoxaprop acid) was synthesized.Hapten conjugated to bovine serum albumin(BSA) with the carbodiimide method was used as immunogens and ovalbumin(OVA) with mixed anhydride method was used as the coating antigens.The New Zealand rabbits were immunized by conjugate of Hapten-BSA and titres of anti-fenoxaprop-p-ethyl serum(1.024×105) were determined by non-competitive indirect enzyme-linked assay procedure.After optimization of the ic-ELISA conditions,such as pH values,inonic strengths,concentrations of methanol,pH 7.5,PBS of 0.3 mol/L Na+,and 10% methanol were determined as optimum assay conditions.The IC50 for fenoxaprop-p-ethyl was(0.084±0.012) mg/L,and lower detection limit(LDL) was(0.0064±0.002) mg/L.The cross-reactivities of other aryloxyphenoxypropionic acid herbicides to the antibodies were less than 0.1% in the assays,except metamifop because of its structures.