Characterization of monoclonal antibody binding site to the polymorphic length of hepatitis B surface antigen synthesized in a cell-free system.
: A combination of two modern biological techniques, hybridoma and recombinant DNA, was described to screen and characterize a monoclonal antibody binding site. Three monoclonal antibodies, M27, M9 and M01 recognizing to a specific domain of the hepatitis B virus surface antigen (HBsAg), were determined by their ability to immunoprecipitate the in vitro synthesized HBsAg in a polymorphic length. Results indicated that the M9 and M01 both recognize the S region of surface antigen while the M27 binds to the pre-S2 region of surface antigen.