Comparison of immunogenicity beween an attenuated Shigella flexneri 2a T32 strain co-expressing CFA/I and CS6 of enterotoxigenic Escherichia coli (ETEC) and a mixture of two attenuated Shigella flexneri 2a T32 strains expressing CFA/I and CS6 re
Objective To select and develop an effective and safe attenuated vaccine against ETEC. Methods A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co expressing colonization factor antigen Ⅰ(CFA/Ⅰ) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/Ⅰ and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses. Results Animals immunized with above strains developed specific serum antibodies and secretory immunoglobulin A(sIgA) aganist CFA/Ⅰ and CS6 of ETEC. The antibody titers of the group only immunized with attenuated Shigella flexneri strain FWL01(pZCF16) are similar or higher than the group immunized with the mixture of two strains FWL01(pZHY21) and FWL01(pZLG6). Conclusion These results show the feasibility of a multivalent vaccine expressing different fimbriae antigens in a live carrier.