Determination of Imidacloprid and Acetamiprid in Leek by Reversed Phase High Performance Liquid Chromatography with Microwave Treatment and Convenient Reverse Solid Phase Dispersion Clean-up
A rapid method has been developed for the determination of two neonicotinoid insecticide residues including imidacloprid and acetamiprid in leek.The samples of leek were treated with microwave to make the enzyme inactive and to eliminate the matrix interferences.Then the pretreated samples were extracted with acetonitrile,further cleaned up by reverse solid phase dispersion,and analyzed by reversed phase HPLC with diode array detector(DAD).Average recoveries of imidacloprid and acetamiprid were found in the range of 95.2%-105.3% and 97.4%-108.8% at three spiking levels from 0.05 mg/kg to 2.0 mg/kg with relative standard deviations of 0.8%-7.8% and 1.3%-8.3%,respectively.Limit of detections of imidacloprid and acetamiprid were 0.0078 mg/kg and 0.0075 mg/kg,while limit of quantifications were 0.026 mg/kg and 0.025 mg/kg,respectively.