Simultaneous Direct Determination of Magnolol and Honokiol by Second Order Derivative Synchronous Fluorimetry
The second order derivative synchronous fluorescence spectra of magnolol,honokiol and their mixture in 0.08%-0.16% methanol solution were studied.The experiment results indicate that their second order derivative synchronous fluorescence spectra were separated Absolutely,which eliminated the disturbance between them.A novel method was developed for the simultaneous direct determination of magnolol and honokiol in mixed solution by second order derivative synchronous fluorescence.For magnolol and honokiol,the linear ranges are 2.8-500.0 μg/L and 4.3-560.0 μg/L,the detection limits are 0.84 μg/L and 1.30 μg/L,and the recoveries are 94.65%-105.58% and 95.09%-104.51%,respectively,the relative standard deviations(RSD) are all under 4.1%.This method has been successfully used to direct determine magnolol and honokiol in the magnolia bark and their supercritical fluid extract simultaneously.