Expression Analysis of Related microRNAs During the in vitro Flowering by qPCR in Amaranthus mangostanus L.
In this experiment in vitro plantlets and the cultures during the in vitro flowering were used as the material to study the expression of related microRNAs during the in vitro flowering by qPCR in Amaranthus mangostanus L.The results showed that the most suitable reference gene was ama-miR159a by NormFinder software analysis.The relative expression levels of ama-miR156c,ama-miR156d and ama-miR156g were downtrend from the young seedling stage to the adult seedling stage,and constantly kept at lower levels during the whole developing process.The relative expression levels of ama-miR156h,ama-miR157b,ama-miR157c,ama-miR157d,ama-miR3 and ama-miR10 were downtrend from the young seedling stage to the flower-bud stage,and decreased to the lowest level at the flower-bud stage.The ama-miR172d showed the opposite expression model with miR156/miR157.The relative expression level of ama-miR319b was upstrend from the young seedling stage to the flower-bud stage.The relative expression level of ama-miR164b was higher at flower-bud stage,then decreased.The relative expression levels of ama-miR166a/miR166g were up-regulation during the whole flowering period.The relative expression levels of ama-miR167b/miR167d and ama-miR4 slightly increased at first and then decreased,and to the lowest at the flower-bud stage,finally increased.Therefore,the microRNAs played the important role in regulating the conversion from the young seedling stage to the adult seedling stage,from the vegetative growth to the reproductive growth and formation of floral organs and so on.