Speciation Analysis of Organotins in Aquatic Products by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry
A method for determination of seven organotins( TMT,MBT,DBT,TBT,MPh T,DPh T,TPh T)in aquatic products was established using high performance liquid chromatography-inductively coupled plasmamass spectrometry( HPLC-ICP-MS). An Eclipse Plus C18 column was used to separate the seven compounds using methanol-acetic acid( with 0. 2% triethylamine V / V) acid as the mobile phase,and the contents of these seven compounds were determined by external standard method using plasma mass spectrometry. The seven organotins compounds were effectively extracted using 30 m L of 30%( V / V) acetic acid-methanol with ultrasound for 10 min in low temperature,centrifuged for 3 min and the 20 m L supernatant was concentrated and diluted with mobile phase to 2 m L,and then separated in 30 min with gradient elution. Linear correlation coefficient for the standard curve of seven kinds of organotins was larger than 0. 995,and detection limit was0. 11- 0. 60 μg / kg. The spiked recoveries ranged from 72. 1% to 111. 7%. The method is feasible for the simultaneous determination of organotins in aquatic products with simple and convenient operation,efficient separation and low detection limit.