Determination of changes in lipoxygenase activity of Korla pear under different storage conditions
In this experiment, determination of lipoxygenase(LOX) activity of Korla pear was studied. The optimal determination conditions were as follows: adding 1.5% PVP and NaH 2 PO 4-Na 2 HPO 4 buffer(pH7.0) into crude enzyme solution, when its temperature reached 35 ℃, adding 40 μL LA solution, then analyzing the enzyme activity at 234 nm wavelength, under these conditions LOX activity is ideal. According to the optimal conditions, the changing rules of LOX activity of the Korla pear were studied during the period of chilling temperature storage, painting film storage and controlled atmosphere storage, the inhibiting effect of controlled atmosphere storage was more evident.