Detection HV gene in patients with HFRS by PCR-ELISA
Objective To develope a fast and sensitive method for detecting Hantavirua (HV) gene in sera of HFRS patients by PCR-ELISA Method The primers labeled with digoxin and biotin and complemented to S segment of HV were synthesized and RT-nested PCR was conducted for detecting HV genes in serum of HFRS patients. The amplified products were analyzed by ELISA. Result The samples from 108 HFRS patients were tested by RT-nested PCR and the positive rate was 62.0%; on the other hand,the positive rate was 81.5% by PCR-ELISA. Conclusion PCR-ELISA is more sensitive in early diagnosis of HFRS.