Ultrasonic-assisted extraction-determination of ceramides from Konjac by HPLC
The optimum extraction condition, isolation and purification methods of ceramides from Konjac were studied. After ultrasonic-assisted extraction, petroleum ether extraction and slica column chromatography, we determined the ceramides by HPLC. The best extraction conditions for ceramides were obtained through orthogonal experiments as follows: 65% ethanol (A), liquid material ratio 1:4 (B), time 25 min (C) and temperature 70 ℃ (D). And the order of effection on the extraction rate was: CBAD. The content of ceramides from Konjac was 0.047% (g/g) with HPLC method.