An Immunoassay Chip Based on Electrowetting on Dielectrics
An immunoassay chip based on electrowetting on dielectrics(EWOD) droplet control method was developed.Basing on the process of immunoassay,the immunoassay chip was designed,and fabricated using indium-tin-oxides(ITO) coated glass as substrate.We tested the controllability performance on droplet actuating of the chip.The experimental results of contact angles of the droplet were closer to the theoretical predictions within 100 V.The largest speed of the droplet movement was up to 3.75 mm/s,and the droplet manipulations were achieved by EWOD on the ITO-coated glasses.By the immunoreaction of rat IgG and goat anti-rat IgG on the immunoassay chip,the possibility of the immunoreaction on the immunoassay chip was validated.The volume of sample is 0.5 μL and the detected time is 20 min.The detected range of the immunoreaction of rat IgG and goat anti-rat IgG is 0.1-20 mg/L.