ISSR Analysis of Citrus reticulata Blanco cv. Bian Gan Germplasm Resources
This study aimed at accurately understanding of resource status and types,taxonomic status and genetic relationship of Guangxi Citrus reticulata Blanco cv.Bian Gan,thus promoting its utilization.Thirty-five accessions of Citrus germplasm,including 21 Guangxi Citrus reticulata Blanco cv.Bian Gan species,2 Vietnam samples and 12 closely related varieties were analysed by using inter-simple sequence repeat(ISSR) and the results showed that 134 fragments of DNA were amplified with 10 primers by using ISSR-PCR,among which 108(81%) fragments were polymorphic.The genetic distance was analyzed,and a tree diagram constructed by using UPGMA algorithm.The genetic similarity coefficients among the accessions were calculated with NTSYS-pc2.10e software and the values were between 0.64 and 1.00.The 35 materials could be clustered into 5 groups at the similarity coefficient level of 0.80,all Citrus reticulata Blanco cv.Bian Gan samples had the greatest genetic similarity with Ponkan and Nanfeng mandarin,indicating that Citrus reticulata Blanco cv.Bian Gan was mandarin.Citrus reticulata Blanco cv.Bian Gan had a near relationship because of a high index which was above 0.90.