Investigation of Potential Markers for Quality Control of Corn Steep Liquor in Penicillin Fermentation by Gas Chromatography-Mass Spectrometry
A gas chromatography-mass spectrometric(GC-MS) technique together with orthogonal partial least-squares discriminant analysis(OPLS-DA) for exploring potential markers of corn steep liquor(CSL) in penicillin fermentation has been developed.44 compositions of CSL were determined by GC-MS.Furthermore,the data sets were mean-centered and pareto-scaling before OPLS-DA analysis.According to loading plot and variable important in the projection(VIP) value,nine potential markers were screened,namely leucine,5-oxo-proline,aspartate,citrate,tyrosine,serine,threonine and glucose.The result suggested that they were related to primary and secondary metabolism in penicillin fermentation,and considered as potential quality specifications for screening excellent raw materials.