Evaluation on Yield and Sugar Characteristics in Transgenic Sugarcane Mediated with SrMV-P_1 Gene from Sugarcane Mosaic Virus
Yield and sugar characteristics of transgenic sugarcane mediated with SrMV-P1 were evaluated for two years based on the pilot experiment of patented transgenic plants approved by National Safety Committee of Genetically Modified Organisms.The results showed that the resistance to ScMV was improved effectively in the transgenic sugarcane mediated with SrMV-P1 gene.ScMV incidences of all transgenic clones,which were investigated for 2 years,were 5% or less,significantly lower than the receptor cultivars(FN95-1702,CK1)and the transgenic clones mediated with only marker gene but without SrMV-P1 gene(P7,CK2).The transgenic sugarcane clone TF64 was not infected by SrMV virus.Besides,the characteristics of yield and sugar in six transgenic sugarcane were much better than those of CK1 and CK2.Furthermore,the results from the fuzzy comprehensive analysis also revealed that transgenic sugarcane clones,compared with FN95-1702 and the P7(CK1 and CK2),had great performance for two years.The transgenic sugarcane TF53 showed the highest yield and sugar contents.Compared to the controls(CK1 and CK2),the yield of TF53 was increased by 32.9%(CK1)and 46.3%(CK2),and sugar content in cane was increased by 2.1%(CK1)and 1.7%(CK2)(absolute value).The highest sugar content in cane was found in TF37 with 15.6%(absolute value).From all the above,we drew the conclusion that resistance of sugarcane mosaic virus could be improved by mediation of SrMV-P1 gene,and the corresponding yield and sugar content was higher than those of control(CK1 and CK2).