Optimized Conditions of Protein Prestained with Reactive Dye in Native Polyacrylamide Gel Electrophoresis
Reactive dyes are introduced to label bovine serum albumin (BSA) before native polyacrylamide gel electrophoresis.The results show that blue KN-R is the best dye in protein staining and the optimum reaction conditions are pH 10.0,60 ℃,50 min.The excellent reproducibility and stoichiometry within the test scope were obtained and the detection limit to BSA was 200 ng.The method is sensitive,simple,direct and convenient to be observed by naked eyes in real-time style and has good application prospects for the use as protein label agent.