The negative of Lamivudine on HBV-DNA in PBMC and its inducement effect against cytokine
Objective To study the therapeutic effect of Lamivudine on the negative effect of HBV-DNA in peripheral blood mononuclear cells(PBMC) and serum and the level of cytokines in serum of the patients with chronic hepatitis B.Methods The total of 81 patients were divided into two groups(A=47, B=34), and treated by Lamivudine, routine medicine, respectively. The levels of HBV-DNA in PBMC and serum and cytokines were all detected before and after treatment.Results After the treatment of Lamivudine for 36 weeks, the total negative conversion rates of HBV-DNA in PBMC and serum of the patients treated with Lamivudine were 55.32%(26/47) and 61.70%(29/47), respectively. The total negative conversion rates of HBV-DNA in PBMC and serum of the patients treated by routine medicine were 26.47%(9/34) and 32.35%(11/34), respectively. There was significant difference between in Lamivudine group and in routine medicine group(P0.01). The negative rates of HBeAg in serum of the patients were 46.81%(22/47) and 68.09%(32/47) at end of 12 weeks and 24 weeks, and were higher than those of routine medicine group(P0.05 and P0.01). The levels of ALT, AST, ALT/AST in serum of the patients after being treated by Lamivudine and routine medicine were down-regulated to (30.1±9.6) U/ml, (32.3±10.7) U/ml, 0.9±0.1 and (48.4±10.7) U/ml, (44.7±11.0) U/ml, 1.1±0.2. After the analysis of variance, the high significant difference was obvious between two groups(P0.01). It was in factor that the high level of IL-6, IL-8 and TNF-α in chronic hepatitis B could be down-regulated to (250.5±33.3) pg/ml, (153.4±22.2) pg/ml, (232.6±21.2) pg/ml by Lamivudine, which was more obvious than that of routine medicine (P0.01). Conclusion Lamivudine has high therapeutic effect on the treatment of HBV-DNA in PBMC and serum and has better therapeutic effect than that of routine therapy. Lamivudine may also have higher down-regulate inflammatory infiltration and secretion in local site caused by chemotactic cytokines and promotional effect on the recovery of liver function.