Optimization of determination conditions for urease in waste degradation process.
To optimize the method for measuring urease activity,single factor experiment and orthogonal test was used based on soil enzymology,with waste sample in landfill. Results showed that the optimal conditions for urease in waste degradation process were as 30℃ of dried temperature,0.5mL toluene,1mL of 10% urea,3mL of 0.1M sodium citrate buffer at pH 5.5, 30℃ of culture temperature and 24h of culture period. Relatively standard deviation(RSD) was less than 0.82% in the verification test,and measured value was 30.48% to 147.28% higher than the traditional method,indicating high precision and feasibility of the method.