A Novel Glucose Biosensor Based on Thin-film Gold Electrode Modified Nanoparticles
To improve the sensitivity and lower the working potential of the biosensor under the condition of no mediator,a new biosensor is introduced and the glucose oxidase is immobilized on the surface of the modified electrode by glutaraldehyde cross-linking.The nano-Pt particles are deposited on the surface of the electrode fabricated by thin-film technology.The analytical characteristics of the biosensor for low concentration are studied at the platinum nanoparticles and ordinary thin-film electrode.The results show the detection limit of the glucose biosenser decreases from 100 to 10 μmol/L and the working potential is 0.4 V.The result exhibits analytical sensitivity of 50.8 nA/(cm2 μmol/L) in the relevant concentration range of 0-1300 μmol/L at the response time 30 s.The RSD is 2.1%.The biosensor also indicates good stability.