Expression of the G glycoprotein from the respiratory syncytial virus field strain by recombinant vaccinia virus
Objective To obtain G glycoprotein from respiratory syncytial virus (RSV) field strain. Methods RSV gene fragment encoding the G glycoprotein from the virus field strain isolated from child with acute respiratory infection in Beijing was inserted into the vaccinia virus vector and the recombinant vaccinia virus expressing the G protein of RSV was obtained by co-infecting this vector with vaccinia virus field strain in cell culture. Rabbits were immunized with the recombinant vaccinia virus expressing RSV G glycoprotein. Western blot, ELISA and plaque reduction assay were used to evaluate the antigenicity, immunogenicity and the biological function of the expressed protein. Results The RSV G glycoprotein was expressed by the recombinant vaccinia virus, which was determined by Western blot with anti-G protein specific monoclonal antibody. The high titer antibodies against G protein and purified RSV were detected from the sera collected from immunized rabbits. And the sera from the immunized rabbits can neutralize the plaque formation of the native RSV. Conclusion The data suggested that the expressed G glycoprotein is similar antigenically and immunogenically with those from native RSV.