Acceleration of hematopoietic recovery of chemotherapeutically lesioned mice by recombinant human macrophage colony stimulating factor expressed in silkworm
Objective To investigate the effect of rhM CSF on hematopoietic recovery of chemotherapeutical lesions. Methods 100mg/kg CTX were ip injected into mice for 3 consecutive days to make the model of chemotherapeutical lesions. Mice were also ip injected with rhM CSF at doses of 0 1 2 0μg/day following the injection of CTX. Results 1 Observation of the peripheral hematological changes showed: The peripheral white blood cell count of treatment group(0 5 2 0 μg/day rhM CSF) was significantly higher than that of the control group at day 8 ( P 0 01). Monocyte and granulocyte counts of treatment group(0 1 2 0 μg/day rhM CSF) were significantly higher than those of the control group at day 8( P 0 01). Among which, 1 0 μg/day rhM CSF was most active. 2 On day 7, the bone marrow CFU GM yield of control group was significantly lower than that of treatment group, and no evident difference was found between the effect of rhM CSF and rhM CSF plus G CSF as stimulating factor. Conclusion rhM CSF could accelerate hematopoietic recovery in chemotherapeutically lesioned mice.