Immunization of SD rats with GBD fusion protein of Streptococcus mutans glucan binding protein-A
Objective To study the specific immune response in serum and salivary of SD rats induced by the GBD fusion protein of Streptococcus mutans glucan binding protein-A. Methods Purified GBD fusion protein of Streptococcus mutans GbpA was used to immune SD rats by subcutaneous injection route. Serum and saliva were collected and were tested by ELISA. Results Immunization with GBD fusion protein were able to induce significant serum IgG antibody and salivary IgA antibody (P0.01). In addition, the titer of anti-GBD in serum at the thirty-eighth day after the first immunization was the highest one. Conclusion GBD fusion protein of Streptococcus mutans glucan binding protein-A has the immunogenicity and is a candidate vaccine.