Reaction between hydrocyanic acid, cyanide ion and ferricytochrome c.
at 280 and 294*4 m,u., the total protein content can be calculated from the expression Total protein (g./100 ml.) = 9-76 (2-2E294.4-E280). Estimation of serum protein concentration from serum specific gravity. The relationship between the serum protein concentration (P, in g./100 ml.) and the serum specific gravity (G) is usually expressed in the form P = a(G-b) where a and b are constants. Sera from 320 cattle have been examined, the serum protein values varying from 4 5 to 11-0 g./ 100 ml. and the specific gravities from 1-0200 to 1*0405. For the specific gravity range 1-0220 to 1*0365 (corresponding to a serum protein range of from 5*5 to 9*6 g.) the equation relating these quantities, obtained using values for 312 sera, was P= 362*0 (G-1*0020) (a = 362-02 + 7-668, b = 1F00197 + 0.000202). Beyond these limits the relationship was no longer linear.