Conditions for protoplast preparation of Ophiocordyceps sinensis
The mycelia of Hirsutella sinensis,the anamorph of O.sinensis,were cultured in liquid flasks and the different parameters for protoplast preparation of H.sinensis were investigated including the enzyme type and its concentration,stabilizer of osmotic pressure and its concentration,pH value of the enzyme and the enzymolysis time.The results showed that protoplasts were obtained with the highest yield of protoplast under the conditions as follows: digested by Yatalase enzyme of 4 mg/ml and Glucanex enzyme of 3 mg·mL-1,1.0 mol·mL-1 KCl as osmotic stabilizer,pH value of enzyme of 6.0 and enzymatic hydrolysis for 3 h.s