Study on cultivation condition of HMS-4 strain producing MnP
This paper research enzyme production and activity of HMS-4 alkaliphilic strain in alkaline liquid medium(pH≈10.5) with various carbons or nitrogen and various ratio of carbons and nitrogen.The result showed that Klason-lignin takes advantage of production of MnP,the activity is 280.45U/L.The activity of MnP is higher in sugar cultivation condition,its activity is 120.36U/L.And its activity is 129.76U/L(8days) at optimal cultivation times.Lower carbon medium is suitable for production,and low nitrogen medium restrain obviously synthesis of the enzymes.