Detection of CaMV 35S promoter by loop-mediated isothermal amplification
After designing the primers and optimizing experimental parameter,a LAMP assay for detecting 35S promoter of cauliflower mosaic virus was established.These primers were proved to be specific and the detective limit was 0.002%.The detection results of 80 samples showed that the positive rate was in agreement with real time PCR assay.The LAMP assay for detecting CaMV 35S promoter may provide a potential tool for products quarantine and food processing,which is of importance in supervising of genetically modified organisms and promoting exportation.