Clonging,Expression Analysis and Genetic Transformation of XRN2 Gene in Tomato
To understand the function of XRN2 gene in tomato,the full length SlXRN2 cDNA was amplified by RT-PCR from tomato mix cDNAs.Expression analysis results showed that SlXRN2 expressed the highest in tomato roots.The expression of XRN2 gene could be up-regulated by wound stress on the leaves.Then SlXRN2 gene driven by the constitutive promoter CaMV35S was directed cloning into plant expression vector pCambia1301-35S,named as pCambia1301-35S-XRN2 and pCambia1301-35S-AsXRN2.Transgenic tomato lines were obtained via agrobacterium-mediated transformation,which would provide significant evidence for further study of XRN2 function in tomato