Determination of Clofencet in Wheat Using Ultrasound-Assisted Extraction by High Performance Liquid Chromatography
A method for the determination of the clofencet of chemical hybridizing agent SQ-1 in wheat has been established by high performance liquid chromatography.SQ-1 was extracted with 75% of ethanol and cleaned up by extraction and stripping technique.Diamonsil C18 column with the mobile phase consisted of methanol-water(60 ∶ 40,V/V,including 0.5% ammonium hydrogen phosphate,pH 2.5) at a flow rate of 1.0 mL/min was used for the detection at wavelength of 283 nm.A good linear relationship was obtained in the range of 0.75-25.0 mg/L with the correlation coefficient 0.9999.The average recoveries of SQ-1 were 84.2%-95.0%,the RSD were 1.0%-3.3%,and the detection limit was 0.075 mg/L.