Assessing biofilm formation by Listeria monocytogenes
Listeria monocytogenes(L. monocytogenes) is a serious food-borne pathogen for immunocompromised individuals. 
L. monocytogenes is capable of producing biofilm on the 
surface of food processing lines and instruments. The biofilm transfers contamination to food 
products and impose risk to public health. Transfers contamination to food products, and 
impose risk hazard to public health. The aim of this study was to investigate biofilm producing 
ability of L. monocytogenes isolates. Microtitre assay was used to measure the amount of biofilm production by ten 
L. monocytogenes isolates from minced chicken / meat, sausages and burgers. Results showed that all 10 
L. monocytogenes isolates were able to form biofilm 
after 24 h at 20 C on polystyrene surface (the common surface in food industries). Some strains 
were capable of forming biofilm more than the others. All strains showed a slight raise in the 
quantities of attached cells over 48 and 72 h. 
L. monocytogenes strains isolated from minced 
chicken, minced meat and burgers were better biofilm-producers comparing to the strains 
isolated from sausages.