Preparation technology of low-molecular-weight fucoidans and their antibacterial activity
In order to prepared low molecular weight fucoidans, the method of ultrasonic-assisted oxidative degradation was used in this work, and hydrogen peroxide was chose as the oxidative agent for its high oxidative capacity. Preparation of the molecular weight by ultrafiltration membrane separation50 ku and 50 ku two low molecular weight component ingredient fucoidans, and not degraded fucoidans antibacterial activity was measured as a control. Meanwhile, the degradation rate of polysaccharides and sulfate group retention were determined as well as the antibacterial activity on Staphylococcus aureus. The experimental results showed that after two-hours degradation, the rate of hydrolysis of polysaccharides was 30.73%, sulfate group retention rate of low molecular weight fucoidans was 24.71% and the diameter of inhabiting circle for Staphylococcus aureus was 25 mm. Therefore, the method of ultrasonic-assisted oxidative degradation could be effective to degrade fucoidans and the obtained low molecular weight fucoidans exhibited good antibacterial biological activity.