Intratypic Nucleotide and Amino Acid Sequence Variations in the L1 Open Reading Frame of Human Papillomavirus Type 6 in Condyloma Acuminata Lesions
PCR was used to determine the human papillomavirus (HPV) in genital wart lesions from patients in PUMC hospital.Type of HPV was identified by restriction fragment length polymorphism analysis The predominant type was HPV6,secondly the HPV11.Eight isolates of HPV6 with different clinical manifestations were chosen for further intratypic nucleotide and amino acid sequence variation analysis.HPV6 L1 genes of eight isolates were amplified and inserted into plamid pMD18-T.By sequencing and phylogenetic analysis,the most frequently observed substitutions in HPV6 L1 ORF were distributed in four discrete regions:SR1(5911～6104),SR2(6217～6273),SR3(6540～6661) and SR4(7062～7250).Some substitutions resulted in non-silent mutation.There had 0 to 3 deduced amino acid changes in different L1 sequences,but which didn't affect immunogenicity of L1 protein obviously.