A physical genome map or Salmonella gallinarum
Objective To construct a physical map of the genome of Salmonella gallinarum 287/91 and compare it with that of Salmonella gallinarum SARB21 and that of Salmanella typhimurium LT2 for identifying genomic differences among strains of the same Salmonella species and genomic differences among bacteria of differ- ent Salmonella species.Methods Pulsed field gel electrophoresis separation of genomic DNA following cleavage with XbaⅠ,AvrⅡor I-CeuⅠ.Tn10 insertion inactivation and double digestion with end labeling techniques were also employed for locating genes and refining the map.Results The genome map of S.gallinarum 287/91 was constructed.This genome shares most insertions and deletions with strain SARB21 relative to S.typhimurium LT2.Condusion S.gallinarum 287/91 and SARB21 have the same genetic contents,the main differences sit in the arrangements of genomic DNA resulting from different rrn operons involved in the homologous recombination, leading to different genome structures.