Antimicrobial activities and biological characteristics of endophytic fungi T4 from Ginkgo biloba L.
Twenty-five endophyte isolates were obtained from healthy roots,stems and leaves of Ginkgo biloba L.The results of preliminary test exhibited 16 endophyte isolates significantly antagonized six plant patho-gens,especially the endophyte T4 strain.The results of mycelium growth rate showed the ferment broth of T4 had strong antagonistic action against Fusarium oxysporum,Cytospora mandshurica,Colletotrichum gloeosporioides,Venturia pirina and Fusarium graminearum,especially against Cytospora mandshurica with 92.31% inhibitory rate.The study on the biological characteristics exhibited that the proper growth temperature of the strain was 28-32℃,and suitable carbon sources were glucose and maltose while the optimal nitrogen source was tryptone.In addition,the strain T4 was preliminarily identified as Fusarium sp.based on morphological characteristics.