Simultaneous Determination of Six Kinds of 4-Hydrobenzoic Acid Ester in Food Using High Performance Liquid Chromatography-Diode Array Detection
A method was developed for the determination of preservatives of six 4-hydrobenzoic acid ester by HPLC-DAD.The samples of 4-hydrobenzoic acid ester were extracted by acetonitrile and purified by LC-C18 SPE.The analytes were separated on an Agilent C18 column,eluted by gradient with methanol and water,detected by diode array detector,and quantified by external standard method.The calibration curve was linear over the concentration range of 1.0-500.0 mg/L(r≥0.9997).The lower limit of quantification in food was 0.0016-0.0081 mg/L.The relative recoveries of six kinds of 4-hydrobenzoic acid ester in vegetables and fruit of surface treatment,vinegar,carbonated beverages,milk,and food fillings were 85.1%-95.4%,and relative standard deviations were 3.6%-13.0%.