The measurement of membrane potential during photosynthesis and during respiration in intact cells of Rhodopseudomonas capsulata by both electrochromism and by permeant ion redistribution.
1. The membrane potential in intact cells of Rhodopseudomonas capsulata during photosynthesis and during dark respiration has been measured by two independent methods. 2. The light-induced and O2-induced shifts in the carotenoid absorption spectrum were measured in the intact cells. The shift was calibrated with K+-diffusion potentials in chromatophores derived from those cells. The light-induced and O2-induced membrane potentials were -290 mV and -230 mV respectively. 3. The energized uptake of butyltriphenylphosphonium ions was measured in the same batch of cells. The light-induced and O2-induced membrane potentials calculated from the Nernst equation were -160 mV and -120 mV respectively. 4. It is concluded that the two kinds of probe measure the electric potentials across different domains of the cytoplasmic membrane, but it is difficult to reconcile the existence of such domains with simple electrical analogues of the membrane and aqueous phases.