Preparation of protoplast from Paecilomyces tenuipes,the anamorph of Cordyceps takaomontana
The optimum condition for protoplast preparation of Paecilomyces tenuipes RCEF3456 was investigated by studying some factors,such as mycelium age,kinds of enzymes and its concentration and enzymolysis time.The results showed that protoplasts were obtained for higher production(8.0×107/mL) under the conditions as follows:the hyphae cultured for 18 h,digested by the compound enzyme solution consisting of 1% Lywallzyme,1% Cellulase "Onozuka"R-10 and 1% snailase for 2.5 h at 30℃.Meanwhile,formation and regeneration of protoplasts were observed,and regeneration rate was 1.2%.