Determination of total lysozyme in honey with ultraviolet spectrophotometry
To establish a UV method for determination of the content of total lysozyme for honey.Honey was dialysed in 0.01 mol/L pH6.5 buffer for 5 days and condensed with PEG.The condensed liquid was centrifugated.Lysozyme was determined at 290 nm in the sample solution.The total lysozyme showed a good linear relationship at 50～250 μg/mL,r=0.9999,the average recovery was 96.0%,RSD=2.64%.The method was accurate and could be used as the quality evaluation of honey.