Study on Chromosome Technique and Karyotype of Tobacco
In the paper,the comparison of five preparatory treatments in chromosome technique of tobacco was carried out. The result showed that the essential oil solution (20%) had the best effect: both the degree of dispersion and the length of chromosomes were proper, and some of the bands were developed clearly.By this treatment,the karyotype of N.glutinosa was analyzed and the karyotype formula was 2n=2x=24=16m(2SAT)+6sm(1SAT)+2t.One satellite on each short arms of the second chromosomes and two satellites on two pairs of short arms of the fifth ones were found,respectively.