Construction of Large Nave Phage Display Antibody Library Using Recombinant System
Phage display antibody library especially large nave antibody library is a reliable technology for the selection of antibody from human.Diverse antibody genes were amplified from lymphocytes collected from normal adult peripheral blood,the antibody genes were inserted into the vector which contains Cre-Loxp recombinant sites to obtain a primary phage display library.A large nave Fab phage display library containing 9×10~(10) clones was constructed after the recombination of primary library completed in the recombinant system.The quality of the library was tested by sequence analysis and selection against several recombinant proteins.Sequence analysis of the randomly picked 96 Ig clones showed the library contains various antibody gene types.Panning of four recombinant proteins was successful and specific clones against one antigen were obtained.