Simultaneous determination of multiple jelly, ice dream,drinks colorants with RP-HPLC method
Objective: To establish RP-HPLC method for simultaneous determination of seven colorants in jelly, ice cream, drinks, e. g. Lemon yellow, amaranth, indigo, carmine, sunset yellow, allura red, blue.Methods: After the samples were polyamide adsorbed, filtered, elution, concentrating dissolved in water,used Agilent C18column(5 μm, 250 mm×3.0 mm) chromatographic column separation, methanol-0.02mol/L ammonium acetate solution as mobile phase, method using gradient elution, change the wavelength determination. Results: Seven colorants had good separation. The correlation coefficients was more than 0.999. The recovery with standard addition was 84.6%~107%, RSD5%. Conclusion: This method is sensitive, rapid, accurate, good repeatability, high sensitivity, good colorants separation, and recovery satisfaction, and it can simultaneously determine seven colorants in jelly, ice cream, drinks.