Gene clone and prokaryotic expression of peanut allergen Ara h1
Ara h1 is one of the major allergens of peanut. Total RNA was extracted from peanut kernel and cDNA was synthesized by reverse transcription. Thereafter the gene of Ara h1 was amplified by the method of PCR using primers designed according to the reported sequence. The PCR products were ligated to a prokaryotic vector pRSET B and transfected into E. coli for expression. The expressed product was partially purified. SDS PAGE and Immunoblot demonstrated the presence of recombinant Ara h1 with IgE binding capacity. This recombinant allergen may be used to neutralize the patients′IgE reactive components as a kind of specific immunotherapy.