Amplification of PCR in Idetification of Intergeneric Hybrids between Anthurium andraeanum and Spathiphyllum wallisii
intergeneric hybrids between Anthurium andraeanum and Spathiphyllum wallisii were identified by using nuclear markers tRNA Leu CAA intergenenic sequence and internal transcribed spacer 2 (ITS2) based on PCR technique.13 sites were obtained by using nuclear markers tRNA Leu CAA intergeneric sequence.No specific-male band was observed in hybrids.New hybrids were visualised in one hybrids.So only one hybrids was identified to be a real intergeneric hybrid.28 bands were amplified by ITS 2 sequence.It showed 3 band types:No specific-male bands type;New bands type;Specific-male and new bands type.6 hybrids were suggested to be the real intergeneric hybrids between A.andraeanum and S.wallisii.Furthemore,the results indicated that these molecular markers can be used for identifying intergeneric hybrids.