Preparation of Stir Bar Sorptive Extraction Based on o-Nitrophenol Imprinted Polymer and Its Extraction Performance
A new stir bar sorptive extraction(SBSE) based on molecularly imprinted polymer(MIP) with o-nitrophenol as template molecule was prepared.The effect of preparation conditions on the extraction performance of MIP-SBSE was studied.Then,a methodology combined SBSE and liquid desorption,followed by high performance liquid chromatography with diode array detection for the direct determination of trace o-nitrophenol(o-NP) and phenolsin in real samples was developed.To achieve the optimum extraction performance,several main parameters including pH value and ionic strength in the sample matrix,extraction and desorption time were investigated.Under the optimized experimental conditions,the method showed good linearity between 3.0 and 200 μg/L for o-NP.The detection limit(S/N=3) and quantification limit(S/N=10) of the proposed method for o-NP was 0.13 and 0.40 μg/L,respectively.The spiked recoveries for o-NP in real samples ranged from 83.4%-120.9% and 59.8%-129.0% for other phenols.