Analyze antigenic homogeneous properties of outer membrane protein from Salmonella spp
Objective To analyze antigenic homogeneous properties of outer membrane protein(OMP) from Salmonella spp.Methods OMPs from cells of Salmonella spp and other enterobacteria and Serratia marces- cens were extracted using 2% Laurylsarcosine sodium salt.The OMP of Salmonella paratyphi A was bonded with Freunds adjuvant,immunized Japan large-ear rabbit in the muscle,celiac,hypodermic,and repeated several times.With immunological double-diffusing method and ELISA,the titer of anti-S,paratyphi A OMP serum was acquired.The antigenic homogeneous properties of OMP from Salmonella spp was identified by Western blot and crass-reaction of rabbit's anti-S,paratyphi A OMP antibody serum about Salmonella paratyphi A by Dot blot- ELISA.Results Rabbit's anti-S,paratyphi A OMP antibody serum can react with OMP deriving from Salmonella spp but not with those of other Gram negative and Gram positive bacteria,even not with fungi.Conclusion There is high homogeneous properties of OMP antigen among Salmonella spp.So it can be used for differentiation diagno- sis in laboratory.