The polymorphisms on exon 11 of luteinizing hormone receptor(LHR) gene and their correlation to production performance in Hu Sheep
The nucleotide sequence and single nucleotide polymorphism on exon 11 of luteinizing hormone receptor(LHR) gene was studied in Hu Sheep by PCR-SSCP.The results showed that only the products amplified by primer P2 and P6 displayed polymorphisms.For primer P2,two genotypes(AA and AB) were detected in Hu Sheep.Sequencing revealed a mutation(G1147A) between genotype of AA and AB.For primer P6,three geno-types(CC,CD and EE) were detected.A mutation of T→A occurred between CC and CD genotypes,and a muta-tion of C→A occurred between CC and EE genotypes.The litter size of AB genotype(2.05±0.76)is higher than that of AA genotype,and the CD genotype's litter size(2.50±0.55) is higher than that of CC and CD genotypes,but no significant difference(P0.05) was found in litter size between AA and AB genotypes or in CC,CD and EE genotypes.