Effects of aerobic exercise and resveratrol on oxidative stress and NF-κBp65 in the livers of type II diabetic rats
In order to probe into the effects of aerobic exercise and resveratrol(Res) on oxidative stress and NF-κBp65 in the livers of type II diabetic(T2DM) rats, the authors established a T2DM rat model by feeding the rats with high sugar and high fat food and injecting a small dose(35 mg/kg) of streptozotocin(STZ) into the rats for 5 weeks, divided 40 class SPF male SD rats randomly into 5 groups, namely, a normal control group(group C), a T2DM group(group D), a "T2DM+exercise" group(group DE), a "T2DM+Res" group(group DR), and a "T2DM+exercise+Res" group(group DER), each of which contained 8 rats. The exercise was unloaded swimming training, done 6 d a week; the Res was orally administrated at a rate of 45 mg/(kg·d), 7 d a week. 7 weeks later, the authors measured the rats' fasting blood glucose(FBG), fasting insulin(FINS), and liver TG, TC, SOD, MDA and TNF-a, and the expression of nucleoproteins nSREBP1, nSREBP2 and NF-κBp65, and revealed the following findings: 1) comparing the rats in groups DE, DR and DER with the rats in group D, FINS and HOMA-IR decreased significantly(P0.05), while FBG showed a trend of decreasing(P0.05); comparing the rats in group DER with the rats in groups DE and DR, FBG, FINS and HOMA-IR decreased further(P0.05); 2) comparing the rats in groups DE, DR and DER with the rats in group D, liver SOD activity increased significantly(P0.05), while MDA content, liver index, nucleoproteins TG, TC, nSREBP1, TNF-a and NF-κBp65 in the livers decreased significantly(P0.05 or P0.01), while SREBP2 showed a trend of decreasing(P0.05); comparing the rats in group DER with the rats in groups DE and DR, all the said indexes decreased further. The said findings indicated the followings: both 7-week aerobic exercise and Res can improve oxidative stress and inflammatory reaction in the livers of T2DM rats; aerobic exercise and Res combined intervention produced an effect more significant than the effect produced by aerobic exercise intervention or Res intervention alone, its mechanism may be realized by suppressing nSREBPs in the livers, thus reducing oxidative stress caused by lipid deposition in the livers, and ultimately suppressing the activation of TNF-a induced by NF-κBp65.