Tissue Culture and Micropropagation of Superior Families of Cinnamomum camphora(L.)Presl.
Two newly-selected superior families of Cinnamomum camphora were used for tissue culture and micropropagation.C.camphora explants displayed the optimum initial growth on the primary media,including improved basic DCR(DCRI)supplemented with 6-BA 0.3 mg/L and NAA 0.05 mg/L.Family X5 showed the highest shoot proliferation rate of 2.69 times on subculture DCRI+6-BA 0.6 mg/L+NAA 0.05 mg/L and family X9 exhibited the highest rate of 3.25 times on subculture DCRI+6-BA 1.0 mg/L+IAA 0.2 mg/L+NAA 0.05 mg/L.An optimization experiment revealed one common rooting medium: 1/2MS+IBA 2.0 mg/L+IAA 1.7 mg/L+6-BA 0.1 mg/L+NAA 0.05 mg/L,for two families.The rooting rate was 96.30%.Suitable techniques and procedures were identified for acclimatization,transplantation and later-period management of tissue-cultured plantlets.The survival rates of two families were 85.2% and 89.7%,respectively.