Retention Behaviors of Strongly Polar Compounds from Traditional Chinese Medicine in Hydrophilic Interaction Chromatography
The retention behaviors of six polar compounds from traditional Chinese medicine were investigated with hydrophilic interaction chromatography ( HILIC) by changing kinds of organic solvent and concentration, additives ( salt and organic acid) in mobile phase and column temperature. The retention curves of six solutes exhibit"U"shape with the change in the concentration of water from 0 to 100% ( V/V) in mobile phase, indicating a mixed-mode of hydrophilic interaction and reversed phase chromatography. The retention times of all solutes increased with the increase of salt concentration,and the retention of the acidic solutes increased with the decrease of the acidity of organic acid,trifluoroacetic acid,formic acid and acetic acid in the same concentration of the additives in the mobile phase. These results might have some instruction role to select the condition in the separation of traditional Chinese medicine.