Comparison of the proteome of Mycobacterium tuberculosis cytosolic basic proteins between multiple drug-resistant stains and sensitive stains
Objective To study cytosolic basic protein variation between multiple drug-resistant M. tuberculosis and sentisitive M. tuberculosis. Methods Two-dimensional electrophoresis (2-CE) were employed to compare the differential expression proteins between 8 multiple drug-resistant strains and 9 sentisitive strains. The differential proteins were digested in gel by enzyme and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The data obtained from pep-tide mass fingerprinting (PMF) were searched using the internet available database. Results; There were ten repeated differential spots were found and six of them were identified in gel as namely, hypothetical protein Rv2159c, hypothetical protein Rv0068, NuoB, IlvN, hypothetical protein Rv0992. Conclusion These proteins are potentially target sites for chemotherapy against M. tuberculosis and new marker in the detection of resistance of M. tuberculosis, at the same time, this study provides further insights into the mechanisms of drug-resistance.