Change of methane production pathway with sediment depth in a lake on the Tibetan plateau
Abstract Methane production is the terminal step in the degradation of organic matter in most anoxic lake sediments. It has been suggested that CH 4 production in lake sediments is dominated by hydrogenotrophic methanogenesis, especially in deeper layers. We investigated the vertical sediment profile from the surface to 50 cm depth in the sediment of Bangong Co, an oligotrophic high altitude lake on the Tibetan plateau. We measured CH 4 production, stable carbon isotopefractionation and the archaeal community structure. We found that the methane production rates, the acetate concentrations and the numbers of bacteria and archaea strongly decreased with sediment depth. The enrichment factor (e) for hydrogenotrophic methanogenesis also decreased with depth, while δ 13 C of acetate stayed fairly constant. The contribution of hydrogenotrophic methanogenesis to total CH 4 production increased with depth from ~ 36% to 100%. Analysis of terminal restriction fragment polymorphism (T-RFLP) of archaeal 16S rRNA genes showed that the relative abundance of aceticlastic ( Methanosaetaceae ) methanogens also decreased with depth disappearing completely at 50 cm depth. Our study firstly showed that the methanogenic pathway and the methanogenic archaeal community systematically changed with sediment depth in a high altitude lake, probably controlled by the availability of easily degradable organic matter.