Cloning and Bioinformatical Analysis of Phospholipase Dα1 Gene and Its Promoter in the Latex of Rubber Tree(Hevea brasiliensis Muell.Arg.)
Phospholipase D alpha 1(PLDα1) is involved in wound signal transduction in plant cells,and is a key component for wound-induced jasmonic acid(JA) production.The investigation of PLDα1 expression would facilitate unraveling the signaling pathway of JA and its regulation of the rubber biosynthesis in Hevea brasiliensis(rubber tree).The PLDα1 gene from the Hevea latex,HbPLDα1,and its promoter were cloned based on the EST sequences using RACE and Genome Walking.The full-length cDNA of HbPLDα1 gene was 2 870 bp with a complete ORF of 2 427 bp.HbPLDα1 had the similar typical conserved domains of plant PLD with those of other plants,and showed the highest identity with those of PLDα1 from Ricinus communis and Jatropha curcas.The promoter of HbPLDα1 was 1 559 bp long,including not only the ordinary cis-acting elements of TATA and CAAT box,but also such cis-acting elements responsive to phytohormones(JA,ABA,GB) and environmental stresses.The data demonstrated that the expressed of HbPLDα1 gene could be regulated by signals of both plant hormones and environmental stresses,and it's suggested that HbPLDα1 might play key roles in the responses to hormone stimulation and environmental stresses for rubber trees.