Expression of PP150 C-terminus and gp52 chimeric protein of human cytomegalovirus
Objective To make up an engineering E.coli strain for expression of protein PP150 C terminus and gp52 protein of human cytomegalovirus. Methods The gene fragment of protein PP150 C terminal 25 amino acid was synthesized and cloned into NcoⅠ/BamHⅠ sites before gp52 gene fragment in plasmid pET28a+gp52, the recombinant plasmid was transformed into E.coli BL21(DE3) for screening the engineering E.coli expressing chimeric protein of PP150 and gp52, the recombinant plasmid was identified by restriction enzyme digestion, and the engineering E.coli was screened by SDS PAGE. The expressed chimeric protein was preliminarily purified and characterized by ELISA test. Results The engineering E.coli expressing PP150 and gp52 chimeric protein was established, and the chimeric protein has good antigenicity. Conclusion The expressed chimeric protein is potential for the detection of anti HCMV IgM, and is of great significance in the prevention of HCMV infection.