Enzymatic degradation of chitosan and preliminary study of chitooligosaccharides
This paper described the enzymatic hydrolysis process for preparing chitooligosaccharides.The effect of reaction time,temperature,pH,enzyme concentration,concentration and deacetylation degree of substrate on chitosan hysrolysis were studied.Experiment results demonstrated the optimal temperature,pH,enzyme concentration,concentration of substrate were 65 ℃,pH 5.2~5.6,7 U/g chitosan,3% respectively.Thin layer chromatography was used to analyze the hydrolysate of chitosan.The main products in the reaction were chitotriose and chitotetraose,with extension of reaction time more glucosamine was detected.Results indicated that average polymerization degree was less than 10 after hydrolyzation for 3 h.