Determination of Statins Residues in Chicken and Egg by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
An analytical method based on ultra performance liquid chromatography tandem mass spectrometry has been developed for the simultaneous determination of 6 statins residues in Chicken and egg. The statins were analyzed on an ACQUITY UPLC BEH C 18 column( 100 mm × 2. 1 mm,1. 7 μm),using the methanol- 5 mmol ammonium acetate( added 0. 1% formic acid) as the mobile phase and a gradient elution program with a cycle time of 20 min,detected by electrospray ionization mass spectrometry in both positive and negative mode with multiple reaction monitoring,quantified by external standard method. The limits of detection( LOD,S / N = 3) and quantification( LOQ,S / N = 10) of 6 statins were 0. 1- 0. 5 μg / kg and 0. 3- 2. 0 μg / kg,respectively. The correlation coefficients of linear calibration curve were over 0. 9910 in the ranges of 1. 0- 100 μg / kg. Average fortified recoveries in Chicken and egg samples were 80. 9%- 97. 2% and 79. 0%- 87. 2% at the spiked level of 20 μg / kg,and the relative standard deviations were 1. 3%- 10. 2% and 1. 5%- 10. 7%,respectively.