Regulation of Ursolic Acid Content in Suspension Culture of Eriobotrya japonica L.
Ursolic acid( UA) is an activate component found in loquat and it has high economic value. The traditional UA extraction methods has limitations. A novel approach of producing UA using loquat cell suspension culture technique would have a significant benefit theoretically and practically. Different culture conditions on cell growth and UA accumulation in loquat cells, including sucrose concentration, plant growth regulators, p H, media volume, inoculums quantity, temperature, light intensity and shaking speed were investigated. The results showed that the weight of fresh cell were 3.577 times under optimal protocol after harvesting for 20 days when compared with the initial inoculums, and the UA content obtained reached 34.993 mg/g DW( Dry weight). The optimal protocol was executed by transferring 8 g/100 m L of initial inoculums to a 130 m L liquid medium containing 30 g/L of carbon source, 0.5 mg/L plant hormone 1-Naphthaleneacetic acid( NAA) with p H6.0. Then loquat cell suspension was kept in a 25 ℃ shaker with 400 lx light intensity and shaking at 130 r/min for 15 days. The reulsts would provide foundations for future industrialized extracting UA from loquats in a large scale.