Optimization of enzymatic fish oil extraction from mackerel viscera by response surface methodology.
The used of neutral protease for enzymatic fish oil extraction from mackerel viscera was studied following an experimental design as a statistical problem solving approach. Plackett–Burman design was used in order to select the most important variables from the simultaneous study on influence of operating and enzymatic hydrolysis conditions. The optimization of enzymatic fish oil extraction using the response surface methodology allowed a study on the influence of the variables (pH, neutral protease concentration and temperature). From the obtained results it could be concluded that the yield of fish oil was clearly enhanced at optimum conditions. Model validation showed a good agreement between experimental results and the predicted responses.