Bioinformatics Analysis and Function Forecast of Two POD Genes in Litchi chinensis Pericarp
Two POD genes were cloned from the pericarp of Litchi chinensis and named as LiPOD1 and LiPOD2,respectively.Analysis indicated that the full length of LiPOD1 was 1 062 bp with an open reading frame encoding 353 amino acids that shared high identities with the POD from Hevea brasiliensis,Populus trichocarpa,Ipomoea batatas(87%,84% and 81%.The full length of LiPOD2 was 990 bp with an open reading frame encoding 329 amino acids that shared high identities with the POD from Gossypium hirsutum,Sesamum indicum,Medicago sativa,Arabidopsis thaliana(88%,87%,85% and 81%).Analysis of conserved domains indicated that LiPOD1 and LiPOD2 both had total conserved domains of POD gene and had heme binding site,active site,substrate binding site and calcium binding site.During the process of skin browning within 72 hours both the browning index and POD activity increased.Analysis of structure of LiPOD1 and LiPOD2 speculated that these two genes played important roles in the process of pericarp senescence and browning in postharvest Litchi chinensis.