Adsorption of ligand on recorded lipase polymers in organic phase
Lipase and lauric acid were added into a copolymer system, which contained PEG400-dimethacrylate, trimethylolpropane trimethacrylate, and a surface-active agent.The polymerization was initiated by ultraviolet irradiation .Then recorded lipase polymers (RLP) of locking lipase conformation were obtained after lauric acid was extracted by a neat organic solvent.The adsorption of RLP in the lauric acid solution was studied.The adsorption isothermal curves were measured at temperature 30—50℃.The results indicated that RLP’s adsorption capacity was far stronger than blank carrier’s and immobilized lipase polymers (ILP)’s,about 8.7 and 3.9 times respectively.The adsorption process followed the Langmuir and Freundlich isothermal adsorption equations.