Application of Sodium Hypochlorite in the Open Tissue Culture of Banana
Added the different conceration of sodium hypochlorite solution(chlorine-antiseptic concentration,similarly hereinafter) into the culture medium of banana,the effective concentration of sodium hypochlorite solution used as the bacterial inhibitor on the open tissue culture of banana,the optimal concentration of multiplication and rooting culture was determined,based on the experimental material made of the differentiated bud of bananas "Brazil" obtained by subcultured.It demonstrated that the effective concentration of sodium hypochlorite was above 0.01%.The bud multiplication and root regeneration in the open banana tissue culture were relatively high at the stages of 0.014% sodium hypochlorite concentration,2.48% and 83.0% which were no significant difference with the traditional way of banana tissue,meanwhile the least damage to the plantlets.