Differential Pulse Voltammetric Determination of Two Antivirus Nucleoside Analogues in Blood of Rat
Differential pulse voltammetry was applied to the simultaneous determination of two antivirus nucleoside analogues,aciclovir and penciclovir,in the rat metabolic blood at an ethylenediamine modified glassy carbon electrode.Moreover,the mechanism of the electrode modification and the electrode reaction was investigated and discussed.In the pH 2.56 Britton-Robinson buffer,both aciclovir and penciclovir had a sensitive irreversible oxidation peak on the modified glassy carbon electrode.The peak potentials were 1.20 V and 1.17 V for these two medicines,respectively.Under the optimal conditions,the peak current was linear with its concentration in the range of 0.20-4.0 mg/L for aciclovir and 0.02-0.40 mg/L for penciclovir.The detection limits were 77 μg/L for aciclovir and 12 μg/L for penciclovir.Furthermore,chemometrics of classical least squares(CLS),principal component regression(PCR) and partial least squares(PLS) were applied to resolve the overlap spectra of the admixture of the two medicines,without troublesome procedures of separation and purification.The proposed method has been applied to the determination of the two antivirus nucleoside analogues in the metabolic blood of mouse.