Liquid Extracting from Cassava Leaves and Its Effects on Growth of Soft-shell Turtles
The liquid extracting from cassava leaves and its effects on turtles' growth are optimized in the study.Firstly,the optimal process of extracting liquid from cassava leaves is determined.The extracting conditions include ethanol density,solid-to-liquid ratio,extracting temperature and extracting time.Secondly,the effects of the liquid of cassava leaves on the growth of soft-shelled turtle by determining the survival rate,growth performance,feed conversion ratio,trophic component of turtles,blood biochemical and immunity indicators are analyzed.The result shows that the optimal process conditions are percent of ethanol density(50%),solid-to-liquid ratio(40:1),extracting temperature(70 ℃),extracting time(2 h).In conclusion,it indicates that the feed with the extracting liquid could improve the turtles' growth rate,decrease the feed coefficient,decrease the content of blood sugar,cholesterol and triglyceride of turtle and decrease MDA of turtle serum,so that the liquid could promote the glycometabolism,fat metabolism and immunity of turtle.Thus,this study not only firstly adds the liquid extracting from the cassava leaves into the turtles' feed,but also provides the theoretic reason for using the liquid as natural and low-toxic feed additive.