ISSR Analysis of 3 Natural Populations of the Wild Banana Distributed in the Middle of Fujian Province Based on NTSYS and STRUCTURE
To improve the studies of genetic background of wild banana in Fujian, 100 wild banana leaf samples were collected from 3 populations in Sanming and Fuzhou, and molecular marker of wild banana was conducted by ISSR technology combining NTSYS, STRUCTURE and POPGENE. The results showed that, 175 stable and clear bands were amplified by 13 primers, and 13.5 bands were obtained by each primer on the average. PCR products mainly presented between 200 bp and 2 000 bp, among which 158 bands were polymorphic, accounted for90.3%. Clustering analysis of genetic structure indicated that 100 wild banana samples including Yanqian, Xinkou and Chibi wild banana were divided into three groups strictly according to geographical distribution. The three groups could be further divided into 3, 3 and 4 subgroups respectively. The parameters of genetic diversity revealed that variation within group was greater than that between groups among the total variation of wild banana populations which existed abundant gene exchange. There was a high level of genetic diversity in wild banana,and Yanqian wild banana present the highest, followed by Xinkou wild banana, Chibi wild banana as the lowest.This study showed Fujian wild banana possessed independent genetic structure, and that water, human beings played key roles in population evolution of wild banana.