Nuclear Magnetic Resonance-based Metabonomic Studies on Urine and Serum from Pr(NO_3)_3-treated Rats
The acute biochemical effects of Pr(NO3)3 were studied by NMR-based metabonomic approach on rats urine and serum.Male Wistar rats were administrated intraperitoneally with Pr(NO3)3 at doses of 2,10 and 50 mg/kg body weight.Urine was collected over a 168 h period after dosing and serum was collected at 48,96 and 168 h period.1H NMR spectra of biofluids(urine and serum) were analyzed by principal components analysis.The results revealed that Pr(NO3)3 caused acute damages on rats liver and kidney,mainly in the liver,and showed a significant dose-dependent relationship.The liver and kidney injury induced by Pr(NO3)3 was characterized by the variations of many endogenous metabolites,such as citrate,succinate,α-ketoglutarate,creatinine,N-trimethyl dioxide,amino acids,lactate,taurine and glucose.