Callus induction and browning prevention of Houttuynia cordata Thunb
In this paper,we used the leaves of Houttuynia cordata Thunb as experiment material to compare the effect of different hormones,anti-browning agents and light on callus inducing and browning.The results indicated that MS addition with 6-BA 0.5 mg·L-1 and 2,4-D 1.0 mg·L-1 was the optimal induction medium.VC and PVP can restrained browning of the explants.The effect of PVP was the best,its optimal concentration was 2 g·L-1.Light had obvious effect on browning of Houttuynia cordata Thunb.Browning of the explants could be restrained obviously without light at the early time of tissue culture.