The Expression and Sequencing of Human Endogenous Retrovirus Long Terminal Repeats in Eosinophilia Cells
To explore the relationship between gene expression of endogenous retrovirus long terminal repeat and eosinphilia,PCR was used to amplify the peripheral blood granulocyte DNA fragments in 20 cases of(eosinophilia,) 1 case of malignant lymphoma and 10 healthy persons as control.The expression of long terminal repeat(LTR) gene was detected by RT-PCR.Denatured high performance liquid chromatography(DHPLC) was used to array the long terminal repeat DNA fragments,and then the nucleotide sequence of LTR was analyzed by automatic sequence meter.The PCR results indicated that the LTR gene fragments were found in eosinophilia patients,RT-PCR revealed that LTR gene was higher expressed in 20 cases of eosinophilia patient while no expression was detected in healthy persons.To contrast the homogeneity of nucleotide sequence of eosinophilia on locus of U3、R、U5 to that of HERV-K strain,there showed nucleotide changes.These results demonstrated that human beings carry human endogenous retrovirus sequences as an integral part of their genomes.There may be a relationship between gene expression of human endogenous retrovirus and eosinophilia developing.The mechanism abode in inducing eosinophilia needs further investigation.