Determination of fluoroquinolones residues in milk from large-scale farms in Anhui Province
A method for the simultaneous separation of fluoroquinolones(FQs) by solid phase extraction(SPE) high performance capillary electrophoresis(HPCE) was developed,and in this way,165 samples from 33 large-scale farms in Anhui were determined.The effects of several factors such as the ion concentration,pH value of running buffer,the voltage and the temperature on separation of samples were investigated.The optimal condi-tion was found by orthogonal experiment.The optimal conditions were as follows.UV detection wavelength,215 nm;running buffer,40 mmol·L-1 Na2HPO4-20 mmol·L-1 citric acid;pH 8.10;separation voltage,20 kV.The re-sults showed that the fluoroquinolones residues can be separated within 7 min,and each had a good linear rela-tionship between concentration and peak area(R2=0.9977-0.9997).The average recoveries were between 71.00%-84.58%%.The RSDs were 0.22%-0.67 %(transition time) and 5.41 %-9.75 %(peak area).The posi-tive rate was 9.1%,and the method for detection of antibiotic FQs in large-scale farms of Anhui was set up.The monitoring and management on milk-producing households should be enhanced.