Determination of Rebaudioside A in steviosides by HPLC
A quantitative method for the determination of rebaudioside A (R-A) in stevia rebaudiana extration by means of HPLC. According to GB 8270-1999, we used external standard method to determine R-A through UV detector and Kromasil NH2 Column (250 mm×4.6 mm i.d., 5 μm) as analysis column, acetonitrile and water (80∶20) as mobile phase. The linear range of R-A was 0.1~1.0 mg/mL. The average recovery was 101.51%,RSD was 1.22%. It is suggested that this method is simple, rapid and good linear for R-A quantitative analysis.