Cloning and Bioinformatics Analysis of MLP2 Gene from the Leaves of in vitro Plantlets of Fortunella crassifolia
The Fc-MLP2 cDNA sequence was cloned from the leaves of Fortunella crassifolia cv.Youxijingan using RT-PCR and RACE techniques.The accession number was JX310276 in GenBank.The cDNA sequence consisted of 908 bp with an intact open reading frame of 669 bp,encoding a polypeptide of 223 amino acids.Bioinformatics analysis showed that the protein was a hydrophobic secretory protein located in extracellular position,with two functional sites,13 phosphorylation sites and a signal peptide.The secondary structure was made of alpha helixes,beta sheets,beta turns and coils.Phylogenetic analysis also indicated that the Fc-MLP2 was close to RlemMLP2 of Citrus jambhiri in genetic relationship.