Construction and Identification of RNAi Vector Containing Cassava SBE I,SBE II Gene and Root Specific Promotor
A 494 bp fragment named SI from the Open Reading Frame(ORF)of SBEⅠ and a 340 bp fragment named SⅡ from the ORF of SBEⅡwere cloned from cassava and fused into a larger fragment named SⅢ with Overlap Extension PCR(SOE PCR).The SⅢ fragment was inserted into the plant expression vector pART27 RNAi in forward and reverse direction;Also the original 35S promotor in the vector was replaced with a root specific promotor Sporamin,and then a coding hairpin RNAi expression vector Sp-pRNAiSⅢ was constructed,which could,lay a foundation to breed new cassava varieties with high amylase content.