Expression variation and significance of Skp2 and p27~(kip1) during serum starvation and release process of Raji cells
Objective: To investigate the expression variation and significance of Skp2 and p27kip1 during serum starvation and release process of Lymphoma cell line Raji cells.Methods: The combination of p27kip1 and Skp2 were detected by Immunoprecipitation in Raji cells.Raji cells were treated by serum starvation and release assay for synchronization purpose.The expression variation of p27kip1,Skp2 were detected by Western blot.Results: The results of immunoprecipitation suggest p27kip1 and Skp2 could form compound in Raji cell.During serum starvation and release process of Raji cells,the albumen amount of Skp2 increased,while the albumen amount of p27kip1 decreased.Conclusion: During serum starvation and release process of Raji cells,the increased synthesis of Skp2 may speed up p27kip1 degradation via the ubiquitin-proteasome pathway,then p27kip1 decreased significantly,and then the cell cycle was put forward.