Simultaneous Recovery of DNA and RNA from Soil Microbe
Isolation of DNA from high purity,large fragment and diversity of soil microbe is the basis for molecular ecology study on community structure.PBS washing procedure and SDS extraction method were chosen to extract DNA and RNA from two types of soils simultaneously.Enzyme digesting,PCR and RT-PCR for the extracted DNA or RNA,and results showed that purity of nucleic acid was suitable for follow-up procedures,which would provide basis for studying of microbial activities and function in soil environment.