Analysis of the phylogenetic tree of human hepatitis B virus genotype D complete nucleotide
Objective To study the phylogenetic relationship of different strains of HBV D genotype complete nucleotide. Methods By HBV full length polymerase chain reaction(PCR) in combination with cloning and nucleotide sequencing assay. A Chinese strain of hepatitis B virus genotype D complete nucleotide clone from a chronic asymptomatic HBV carrier of infectious HBV strain of genotype D was sequenced, and GenBank accession of its complete nucleotide sequence is AF280817. Phylogenetic tree was obtained by analysis of complete genes of 30 strains of HBV genotype D sequences from NCBI by use of DNASIS and CLUSTALW. Results The evolutional distance of this Chinese HBV strain of genotype D is the most homogenous to four Swedish HBV strains of genotype D in 30 strains of HBV genotype D published by GenBank. Mediteranean area and Europe countries but not Asia are predominant geographical region of HBV genotype D. Conclusion The discrepancy of evolution of HBV strain of genotype D result from the origin of virus, the distinctness of migratory orientation of virus and the long choice of virus in the host of different heredity and immune idiosyncracy were discussed. [