Reference gene selection for real-time PCR normalization in tea callus
In order to obtain suitable reference genes for analysis of the related gene expression in calli of the control and treatment groups, 7 housekeeping genes from the Gen Bank and ESTs of c DNA library of Camellia sinensis young roots(with complete open reading frame)were chosen for primer design. After analysis the amplification efficiency and primers specificity, the expression levels of the 7 housekeeping genes in tea calli of the control and treatment groups at different growth stages were determined, and their stabilities of expressions were analyzed using ge Norm and Norm Finder softwares. As a result, β-actin and GAPDH are suitable reference genes under this condition.