Genetic diversity of Conidiobolus coronatus from some regions of China
In order to study genetic diversity of Conidiobolus coronatus isolated from different geographical origin in China,genetic diversities of 131 C.coronatus isolates,randomly sampled from 5 Provinces(Anhui,Shandong,Gueizhou,Shanxi and Shanxi)in China,were analyzed using RAPD(random amplified polymorphic DNA) and ISSR(inter-simple sequence repeat)markers.By using 4 selected RAPD and 3 selected ISSR primers,131 DNA bands were amplified,in which 129 bands showed polymorphism with a rate of 98.4%.The coefficient ranges of genetic similarity of the RAPD and ISSR markers were 0.59-0.99,which indicated that the abundant genetic diversities existed among the tested C.coronatus.UPGMA cluster analysis showed that these isolates were divided into 4 groups,and genetic similarities among C.coronatus isolates were partially related with geo-graphical sources.Based on the results,RAPD and ISSR markers could be applied to detecting genetic diversity among C.coronatus isolates.