Extraction and content determination of polysaccharide in Henna
To study the extraction and content determination of polysaccharide in Henna,the polysaccharide was extracted by the method of water extraction and ethanol deposition after removal of its protein by using chloroform and n-butanol on the proportion of 4∶1.Anthrone-sulfuric acid colorimetry was applied to determine the content of polysaccharide.The results indicate that the contents of polysaccharide of leaves and flowers in Henna were 8.13% and 8.98%,respectively.