Development of AFLP Linkage Marker and Location Analysis of Gynoecy Gene from South China-type Cucumber
In this experiment,F2 population was built by using the South China-type gynoecious cucumber,B36,as female parent and weak female cucumber,S6,as male parent.Sixty-four pairs of primers were screened in the F2 population and an amplified fragment length polymorphism(AFLP)molecular marker linked to the all female trait was found and the segregation ratio of female and weak female fitted 3 ∶ 1 in F2 population,suggesting that gynoecy was a quality trait.The analysis results using the biology software,JoinMapV3.0,indicated that the genetic distance between the marker and gynoecious gene was 4.9 cM.In addition,Bioinformatics analysis showed that the marker was located in the terminal of the sixth number chromosome of cucumber genome.At the same time,we selected two pairs of primers in far promoter region of CsACS1G gene and test F2 plants from the progeny of B36 and S6 using a pair of primers by PCR.And the experiment results showed that there was no linkage between the amplified fragments and the gynoecious trait,which suggested that the gynoecious trait of South China-type gynoecious cucumber(B36)was controlled by a new gynoecious gene.