Analysis of Chiral Secondary Aromatic Alcohols by Capillary Electrophoresis Using Sulfated β-Cyclodextrin as Chiral Selector
A convenient CZE method was developed to separate 7 chiral secondary aromatic alcohols using sulfated β-cyclodextrin(S-β-CD)as chiral selector.The baseline separation of the enantiomers of these secondary aromatic alcohols was obtained under the optimal conditions,such as 6% S-β-CD,pH 2.5,20 mmol/L background electrolyte of phosphate acid-triethanolamine,at-15 kV,20 ℃ and 214 nm UV detection.The established method was also successfully applied to determine the enantiomeric excesses value(% ee)of products of asymmetric transfer hydrogenation reactions.It was found that accuracy of this method was in accordance with that of HPLC and GC method.Moreover,this method is simple,precise and can be applied to the chiral separation of secondary aromatic alcohols enantiomers and determination of their optical purity.