Targeted Cleavage of the Human Cytomegalovirus ul54 Gene mRNA by M1GS
Guide sequences(GSs) are small RNA molecules that consist of a sequence complementary to a targeted mRNA and render the targeted RNA susceptible to degradation by the catalytic RNA subunit of RNase P from Escherichia coli(M1 RNA).A sequence-specific ribozyme(T7-M1GS) derived from GSs and M1 RNA was used to target a region of ul54 gene D segment mRNA of human cytomegalovirus(HCMV).Through the experiments about targeted cleavage in vitro and in vivo of the HCMV ul54 gene D segment mRNA by T7-M1GS,the results showed that T7-M1GS can effectively cleave ul54 mRNA.This provides an experimental basis for treatment of HCMV by ribozymes.