Formation and characterization of b cyclodextrin (b CD) polyethyleneglycol (PEG) polyethyleneimine (PEI) coated Fe3O4 nanoparticles for loading and releasing 5 Fluorouracil drug.
In this work, b cyclodextrin (b CD) polyethyleneglycol (PEG) polyethyleneimine (PEI) coated iron oxide nanoparticles (Fe3O4 b CD PEG PEI) were developed as drug carriers for drug delivery applications. The 5 Fluorouracil (5 FU) was chosen as model drug molecule. The developed nanoparticles (Fe3O4 b CD PEG PEI) were characterized by various techniques such as Fourier transform infrared (FTIR) spectroscopy, X ray diffraction (XRD) Scanning electron microscopy (SEM) transmission electron microscopy (TEM) and vibrating sample magnetometry (VSM) The average particles size range of 5 FU loaded Fe3O4 b CD, Fe3O4 b CD PEG and Fe3O4 b CD PEG PEI nanoparticles were from 151 to 300nm and zeta potential value of nanoparticles were from 43mV to 20mV as measured using Malvern Zetasizer. Finally, encapsulation efficiency (EE) loading capacity (LC) and in vitro drug release performance of 5 FU drug loaded Fe3O4 b CD, Fe3O4 b CD PEG and Fe3O4 b CD PEG PEI nanoparticles was evaluated by UV vis spectroscopy. In vitro cytotoxicity tests investigated by MTT assay indicate that 5 FU loaded Fe3O4 b CD PEG PEI nanoparticles were toxic to cancer cells and non toxic to normal cells. The in vitro release behavior of 5 FU from drug (5 FU) loaded Fe3O4 b CD PEG PEI composite at different pH values and temperature was studied. It was found that 5 FU was released faster in pH 6.8 than in the acidic mediums (pH 1.2) and the released quantity was higher. Therefore, the newly prepared Fe3O4 b CD PEG PEI carrier exhibits a promising potential capability for anticancer drug delivery in tumor therapy.
Biomedicine pharmacotherapy Biomedecine pharmacotherapie