Chemical composition, antioxidant and antimicrobial activity of guavirova (Campomanesia xanthocarpa Berg) seed extracts obtained by supercritical CO2 and compressed n butane
Abstract This work reports the chemical characterization, antioxidant and antimicrobial activity of Campomanesia xanthocarpa seed extracts obtained from compressed n butane and supercritical CO 2 extraction. For supercritical CO 2 the extraction condition was 40 degC and 250 bar, while for n butane 35 degC and 10 bar. The total phenolic compound content was 68.58 1.15 mg g 1 for n butane extract and 17.18 2.44 mg g 1 for supercritical CO 2 Both extracts were rich in terpenoids, especially b caryophyllene, while n butane extract had a larger content of flavonoids than supercritical CO 2 The antioxidant activity was tested with FRAP, DPPH and deoxyribose assay, with n butane extracts exhibiting better results. Antimicrobial activity was verified for both extracts against Pseudomonas aeruginosa however, supercritical CO 2 extract afforded a better performance, reaching 5.0 mg mL 1 for MIC and 10.0 mg mL 1 for MBC, while n butane extract showed 40.0 mg mL 1 in both antimicrobial tests. Higher extraction yields were obtained using n butane, which also presented higher antioxidant activity, while supercritical CO 2 extracts afforded the highest antimicrobial activity.