Real time DNA Amplification and Detection System Based on a CMOS Image Sensor.
In the present study, we developed a polypropylene well integrated complementary metal oxide semiconductor (CMOS) platform to perform the loop mediated isothermal amplification (LAMP) technique for real time DNA amplification and detection simultaneously. An amplification coupled detection system directly measures the photon number changes based on the generation of magnesium pyrophosphate and color changes. The photon number decreases during the amplification process. The CMOS image sensor observes the photons and converts into digital units with the aid of an analog to digital converter (ADC) In addition, UV spectral studies, optical color intensity detection, pH analysis, and electrophoresis detection were carried out to prove the efficiency of the CMOS sensor based the LAMP system. Moreover, Clostridium perfringens was utilized as proof of concept detection for the new system. We anticipate that this CMOS image sensor based LAMP method will enable the creation of cost effective, label free, optical, real time and portable molecular diagnostic devices.
Analytical sciences the international journal of the Japan Society for Analytical Chemistry