Porcine antigen specific IFN g ELISpot as a potentially valuable tool for monitoring cellular immune responses in pig to non human primate islet xenotransplantation
Recent progress in xenotransplantation of porcine islets to non human primates (NHPs) gives hope for human clinical trials in the near future. Thus, implementation of an appropriate monitoring method to detect the development of detrimental porcine antigen specific cellular immune responses is necessary. The enzyme linked immunospot (ELISpot) assay has been widely used to monitor antigen specific alloreactive T cell responses in humans; however, the utility of porcine islet specific ELISpot assay has not yet been thoroughly evaluated for pig to NHPs intraportal islet xenotransplantation.
Xenotransplantation