Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi.
A straightforward synthesis of gold nanoparticles (AuNps) is achieved by novel probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi via an intracellular membrane bound mechanism. The bioreduction of HAuCl4 into AuNps was verified by ultraviolet visible spectrophotometry at ~540nm. AuNps were spherical with varying sizes of 5 30nm. AuNps maintained an average crystallite size of 13nm and demonstrated long term stability in physiological buffer and biological media. Furthermore, the protective capping layer consisted of amino acid residues and surface bound proteins rendered them non toxic to murine macrophage (RAW264.7) and human colorectal adenocarcinoma (HT29) cell lines. Finally, biosynthesized AuNps served as superior free radical scavengers against 2,2 diphenyl 1 picrylhydrazyl (DPPH) in contrast to their corresponding gold salt. In short, this green synthesis is cost effective and advantageous for the development of a new class of probiotics mediated and non toxic carriers in targeted drug delivery systems, cancer diagnostic, photothermal therapy, biosensing, and medical imaging.
Enzyme and microbial technology