Synthesis and Characterization of a Walnut Peptides Zinc Complex and Its Antiproliferative Activity against Human Breast Carcinoma Cells through the Induction of Apoptosis.
The walnut peptides and zinc ions were combined to generate a walnut peptides zinc complex (WP1 Zn) with enhanced antiproliferative ability as well as reduced toxicity. The result indicated that Zn ions were successfully combined with WP1 through Zn N and Zn O covalent bonds. WP1 Zn compounds exhibited strong antiproliferative ability against the selected human cell lines, especially MCF 7 cells, whose survival rate reduced to 20.02% after exposure to 300 mg/mL of WP1 Zn for 48 h. WP1 Zn inhibited MCF 7 cell proliferation through inducing cell apoptosis and cell cycle arrest. The results indicated that WP1 Zn induced MCF 7 cell apoptosis via the ROS triggered mitochondrial mediated pathway and cell surface receptor mediated pathway. Our work is the first attempt to elucidate the synergic effect of novel walnut peptides and Zn and with the hope of better understanding the antiproliferative action of bioactive peptides and a zinc complex and support the potential application of WP1 Zn as a functional food ingredient or complementary medicine.
Journal of agricultural and food chemistry