Nanoscale Metal Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells.
We report the design of a phosphorescence/fluorescence dual emissive nanoscale metal organic framework (NMOF) R UiO, as an intracellular oxygen (O2) sensor. R UiO contains a Pt(II) porphyrin ligand as an O2 sensitive probe and a Rhodamine B isothiocyanate ligand as an O2 insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R UiO as an intracellular O2 biosensor. This work is the first report of a NMOF based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.
Journal of the American Chemical Society