In vitro and ex vivo strategies for intracellular delivery
Intracellular delivery of materials has become a critical component of genome editing approaches, ex vivo cell based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane disruption based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory on chip technology in advancing the field.
Nature