Ultra sensitive biosensor based on genetically engineered acetylcholinesterase immobilized in poly (vinyl alcohol)/Fe Ni alloy nanocomposite for phosmet detection in olive oil.
An ultra sensitive screen printed biosensor was successfully developed for phosmet detection in olive oil, based on a genetically engineered acetylcholinesterase (AChE) immobilized in a azide unit water pendant polyvinyl alcohol (PVA AWP)/Fe Ni alloy nanocomposite. Fe Ni not only allowed amplifying the response current but also lowering the applied potential from 80 mV to 30 mV vs Ag/AgCl. The biosensor showed a very good analytical performance for phosmet detection, with a detection limit of 0.1 nM. This detection limit is lower than the allowable concentrations set by international regulations. In addition to the good reproducibility, operational and storage stability, the developed biosensor was successfully used for the determination of phosmet in olive oil samples without any laborious pre treatment. The phosmet recovery rate was about 96% after a simple liquid liquid extraction.
Food chemistry