Production of Omega 3 Fatty Acid Ethyl Esters from Menhaden Oil Using Proteus vulgaris Lipase Mediated One Step Transesterification and Urea Complexation
An organic solvent stable lipase from Proteus vulgaris K80 was used to produce the omega 3 polyunsaturated fatty acid ethyl esters (o 3 PUFA EEs) First, the lyophilized recombinant lipase K80 (LyoK80) was used to perform the transesterification reaction of menhaden oil and ethanol. LyoK80 produced the o 3 PUFA EEs with a conversion yield of 82 in the presence of 20 water content via a three step ethanol feeding process; however, in a non aqueous condition, LyoK80 produced only a slight amount of the o 3 PUFA EEs. To enhance its reaction properties, the lipase K80 was immobilized on a hydrophobic bead to derive ImmK80; the biochemical properties and substrate specificity of ImmK80 are similar to those of LyoK80. ImmK80 was then used to produce o 3 PUFA EEs in accordance with the same transesterification reaction. Unlike LyoK80, ImmK80 achieved a high o 3 PUFA EE conversion yield of 86 under a non aqueous system via a one step ethanol feeding reaction. The o 3 PUFA EEs were purified up to 92 using a urea complexation method.
Applied Biochemistry and Biotechnology