Use of the Biphasic 13C Sucrose/Glucose Breath Test to Assess Sucrose Maldigestion in Adults with Functional Bowel Disorders
Sucrase insufficiency has been observed in children with of functional bowel disorders (FBD) and symptoms of dietary carbohydrate intolerance may be indistinguishable from those of FBD. A two phase 13C sucrose/13C glucose breath test (13C S/GBT) was used to assess sucrase activity because disaccharidase assays are seldom performed in adults. When 13C sucrose is hydrolyzed to liberate monosaccharides, oxidation to 13CO2 is a proportional indicator of sucrase activity. Subsequently, 13C glucose oxidation rate was determined after a secondary substrate ingestion (superdose) to adjust for individual habitus effects (Phase II) 13CO2 enrichment recovery ratio from 13C sucrose and secondary 13C glucose loads reflect the individualized sucrase activity [Coefficient of Glucose Oxidation for Sucrose (CGO S) To determine if sucrase insufficiency could be a factor in FBD, 13C S/GBT was validated using subjects with known sucrase gene mutation status by comparing 13CO2 breath enrichment with plasma 13C glucose enrichment. 13C S/GBT was used to assess sucrose digestion in FBD patients and asymptomatic controls. 13CO2 breath enrichment correlated with the appearance of 13C sucrose derived glucose in plasma (r 2 0.80) Mean, control group CGO S enrichment outcomes were 1.01 at 60' 0.92 at 75' and 0.96 at mean 60' 75' with normal CGO S defined as >0.85 (95% C.I. In contrast, FBD patients demonstrated lower CGO S values of 0.77 at 60' 0.77 at 75' and 0.76 at mean 60' 75' (Chi Square: 6.55; p 0.01) which points to sucrose maldigestion as a cause of FBD.
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