Cell membrane chromatography coupled with UHPLC ESI MS/MS method to screen target components from Peucedanum praeruptorum Dunn acting on a1A adrenergic receptor.
Peucedanum praeruptorum Dunn (BaiHuaQianHu in Chinese) is a traditional Chinese medicine that has a long history of use in China. In this study, HEK 293 a1A adrenergic cell membrane chromatography was coupled with UHPLC ESI MS/MS and successfully used to identify active components from Peucedanum praeruptorum Dunn. Paeruptorin A, paeruptorin B, and paeruptorin C were identified with a1A adrenergic receptor activity. Pharmacological assays showed that tamsulosin hydrochloride, paeruptorin A, paeruptorin B, and paeruptorin C in concentrations of 1x10( 8) to 1x10( 4)mol/mL could relax prostate strips pre contracted with adrenalin in a concentration dependent manner. Therefore, the HEK293 a1A cell membrane chromatography coupled UHPLC ESI MS/MS system may be a potentially useful drug discovery method for screening for medicinal herbal components with a1A adrenergic receptor inhibitory activity.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences