A Two Photon Fluorescent Probe for Lysosomal Thiols in Live Cells and Tissues
Lysosome specific fluorescent probes are exclusive to elucidate the functions of lysosomal thiols. Moreover, two photon microscopy offers advantages of less phototoxicity, better three dimensional spatial localization, deeper penetration depth and lower self absorption. However, such fluorescent probes for thiols are still rare. In this work, an efficient two photon fluorophore 1,8 naphthalimide based probe conjugating a 2,4 dinitrobenzenesulfonyl chloride and morpholine was designed and synthesized, which exhibited high selectivity and sensitivity towards lysosomal thiols by turn on fluorescence method quantitatively and was successfully applied to the imaging of thiols in live cells and tissues by two photon microscopy.
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