Identification of cardiac related circulating microRNA profile in human chronic heart failure
Background During chronic heart failure, levels of circulating miRNAs endued with characteristics of diseased cells could be identified as biomarkers. In this study, we sought to identify cardiac related circulating miRNAs as biomarkers of failing heart. Methods Total RNA of plasma and heart samples was extracted from 10 normal controls and 14 patients with chronic heart failure. Microarray was applied for miRNA profiles. Validation and organ/tissue distribution analysis was performed by qRT PCR. In addition, bioinformatics analysis was performed to understand the critical roles of these cardiac related circulating miRNAs in heart failure. Results Results showed that levels of more than half of the miRNAs dysregulated in heart failed to show any differences in plasma. Meanwhile, more than 90% of the miRNAs dysregulated in plasma remained stable in heart. Four cardiac fibroblast derived miRNAs (miR 660 3p, miR 665, miR 1285 3p and miR 4491) were found significantly upregulated in heart and plasma during heart failure. These 4 miRNAs strongly discriminated patients from controls, and 3 of them showed significant correlations with LVEF. Conclusions This study provides global profiles of miRNAs changes in plasma and failing heart, and using a circulation tissue miRNA profiling comparison model, we successfully identify 3 cardiac related circulating miRNAs as potential biomarkers for diagnosis of heart failure.
Oncotarget