Nanoparticle mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.
A new biomolecular quantitation method, nanoparticle mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich type assay system were transformed into a near infrared (NIR) laser driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle mediated photothermal effect for bioassays.
Nanoscale