Cell Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane.
Eukaryotic cell free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.
Langmuir the ACS journal of surfaces and colloids