Optimising Golgi Cox staining for use with perfusion fixed brain tissue validated in the zQ175 mouse model of Huntington's disease
Highlights A modified reliable Golgi Cox method is described. It is inexpensive and can be performed in any neuroscience lab. Perfusion fixation with 4% paraformaldehyde yields good results. Pre sectioning of perfusion fixed brain tissue is neither required nor preferred for modified Golgi Cox staining. We demonstrate reliable changes in striatal dendritic morphology in the Q175 mouse model of Huntington's disease.
Journal of Neuroscience Methods