Separation and Identification of Swertiamarin from Enicostema axillare Lam.Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance Mass Spectrometry*
Separation and Identification of Swertiamarin from Enicostema axillare Lam.Raynal by Centrifugal Partition Chromatography and Nuclear Magnetic Resonance Mass Spectrometry* Enicostema axillare, a bitter herb, has been used for the treatment of diabetes in traditional system of medicine in India. Swertiamarin, a bitter and potent hepatoprotective iridoid glucoside ~20 mg) was isolated by Centrifugal Partition Chromatography coupled with Diode Array Detector (DAD) from the crude methanolic extract (1.0 g) of its aerial parts using a biphasic solvent system composed of chloroformmethanol water (86:74:20, v/v) in ascending mode for the first time. The purity of the compound was found to be >97% The structure of swertiamarin was determined using spectroscopic data mainly NMR (1H, 13C, DEPT, HSQC and HMBC) and ESI MS. Compound was also identified by comparing 13C NMR data with those reported and some of the 13C NMR values were revised.