Ruthenibacterium lactatiformans gen. nov. sp. nov. an anaerobic, lactate producing member of the family Ruminococcaceae isolated from human faeces.
Two novel strains of Gram stain negative, rod shaped, obligately anaerobic, non spore forming, non motile bacteria were isolated from the faeces of healthy human subjects. The strains, designated as 585 1T and 668, were characterized by mesophilic fermentative metabolism, production of d lactic acid, succinic acid and acetic acid as end products of d glucose fermentation, prevalence of C18 1o9, C18 1o9 aldehyde, C16 0 and C16 1o7c fatty acids, presence of glycine, glutamic acid, lysine, alanine and aspartic acid in the petidoglycan peptide moiety and lack of respiratory quinones. Whole genome sequencing revealed the DNA G+C content was 56.4 56.6 mol% The complete 16S rRNA gene sequences of the two strains shared 91.7/91.6 similarity with Anaerofilum pentosovorans FaeT, 91.3/91.2 with Gemmiger formicilis ATCC 27749T and 88.9/88.8 with Faecalibacterium prausnitzii ATCC 27768T. On the basis of chemotaxonomic and genomic properties it was concluded that the strains represent a novel species in a new genus within the family Ruminococcaceae, for which the name Ruthenibacterium lactatiformans gen. nov. sp. nov. is proposed. The type strain of Ruthenibacterium lactatiformans is 585 1T =DSM 100348T=VKM B 2901T)
International journal of systematic and evolutionary microbiology