Flow Cytometry, a Versatile Tool for Diagnosis and Monitoring of Primary Immunodeficiencies
ABSTRACT Genetic defects of the immune system are referred to as primary immunodeficiencies (PIDs) These immunodeficiencies are clinically and immunologically heterogeneous and, therefore, pose a challenge not only for the clinician but also for the diagnostic immunologist. There are several methodological tools available for evaluation and monitoring of patients with PIDs, and of these tools, flow cytometry has gained prominence, both for phenotyping and functional assays. Flow cytometry allows real time analysis of cellular composition, cell signaling, and other relevant immunological pathways, providing an accessible tool for rapid diagnostic and prognostic assessment. This minireview provides an overview of the use of flow cytometry in disease specific diagnosis of PIDs, in addition to other broader applications, which include immune phenotyping and cellular functional measurements.
Clinical and Vaccine Immunology