Electrochemical gene sensor based on a glassy carbon electrode modified with hemin functionalized reduced graphene oxide and gold nanoparticle immobilized probe DNA
AbstractThe authors describe an electrochemical DNA biosensor based on a glassy carbon electrode modified with gold nanoparticles (AuNPs) and reduced graphene oxide that was functionalized with hemin (hemin rGO) Capture DNA (a 21 mer) is immobilized on the AuNPs via gold thiol chemistry. The detection scheme relies on the measurement of the differential pulse voltammetric current change of the oxidation reduction reaction of hemin before and after DNA hybridization has occurred. The decrease in the voltammetric currents of iron (III) (best measured at 0.3 V vs. Ag/AgCl) is linearly related to the concentration of complementary DNA. The calibration plot covers the 1.0 aM to 0.1 pM concentration range, and the detection limit is as low as 0.14 aM (at a signal to noise ratio of 3) The biosensor was successfully applied to the direct determination of complementary DNA in self prepared samples by using the standard addition method. Graphical abstractAn electrochemical DNA biosensor was developed on a platform of gold nanoparticles (AuNPs) and hemin functionalized reduced graphene oxide (hemin rGO) The principle of DNA detection is based on the voltammetric current change of oxidation reduction reaction of iron (III) at the core of hemin before and after DNA hybridization.
Microchimica Acta