Fluorescence staining of salicylaldehyde azine, and applications in the determination of potassium tert butoxide
Salicylaldehyde azine (1) with an aggregation induced emission (AIE) function, was synthesized from salicylaldehyde and characterized by Fourier transform infrared spectroscopy (FT IR) nuclear magnetic resonance (NMR) high resolution mass spectrometry (HRMS) and X ray single crystal diffraction. Compound 1 exhibited high thermal stability and good light emitting behavior in the solid state. It could be preferably used to dye cellulose and KBr. No obvious changes in fluorescence intensity occurred when compound 1 was dissolved in different pH buffer solutions. The addition of TFA led to slight fluorescence quenching, and the fluorescence intensity gradually increased with increasing potassium tert butoxide solution concentrations. The fluorescence intensity of compound 1 showed a good linear relationship with the concentration of potassium tert butoxide (0.5 4.0 x 10 4 M) y 99.193x 93.599, R2 0.9902; the limit of detection (LOD) was 1.07 x 10 7 M. It could measure the content of potassium tert butoxide with a relative standard deviation (RSD) value of 1.5%