Phylogenetic evidence for intratypic recombinant events in a novel human adenovirus C that causes severe acute respiratory infection in children
Human adenoviruses (HAdVs) are prevalent in hospitalized children with severe acute respiratory infection (SARI) Here, we report a unique recombinant HAdV strain (CBJ113) isolated from a HAdV positive child with SARI. The whole genome sequence was determined using Sanger sequencing and high throughput sequencing. A phylogenetic analysis of the complete genome indicated that the CBJ113 strain shares a common origin with HAdV C2, HAdV C6, HAdV C1, HAdV C5, and HAdV C57 and formed a novel subclade on the same branch as other HAdV C subtypes. BootScan and single nucleotide polymorphism analyses showed that the CBJ113 genome has an intra subtype recombinant structure and comprises gene regions mainly originating from two circulating viral strains: HAdV 1 and HAdV 2. The parental penton base, pVI, and DBP genes of the recombinant strain clustered with the HAdV 1 prototype strain, and the E1B, hexon, fiber, and 100 K genes of the recombinant clustered within the HAdV 2 subtype, meanwhile the E4orf1 and DNA polymerase genes of the recombinant shared the greatest similarity with those of HAdV 5 and HAdV 6, respectively. All of these findings provide insight into our understanding of the dynamics of the complexity of the HAdV C epidemic. More extensive studies should address the pathogenicity and clinical characteristics of the novel recombinant.
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