A Hybrid Approach for Segmentation and Tracking of Myxococcus Xanthus Swarms
Cell segmentation and motion tracking in time lapse images are fundamental problems in computer vision, and are also crucial for various biomedical studies. Myxococcus xanthus is a type of rod like cells with highly coordinated motion. The segmentation and tracking of M. xanthus are challenging, because cells may touch tightly and form dense swarms that are difficult to identify individually in an accurate manner. The known cell tracking approaches mainly fall into two frameworks, detection association and model evolution, each having its own advantages and disadvantages. In this paper, we propose a new hybrid framework combining these two frameworks into one and leveraging their complementary advantages. Also, we propose an active contour model based on the Ribbon Snake, which is seamlessly integrated with our hybrid framework. Evaluated by 10 different datasets, our approach achieves considerable improvement over the state of the art cell tracking algorithms on identifying complete cell trajectories, and higher segmentation accuracy than performing segmentation in individual 2D images.
IEEE Transactions on Medical Imaging