2,2',4,4' Tetrabromodiphenyl ether injures cell viability and mitochondrial function of mouse spermatocytes by decreasing mitochondrial proteins Atp5b and Uqcrc1.
Our object was to explore direct effects and mechanism of BDE47 on GC2 (immortalized mouse spermatocyte) GC2 were exposed to DMSO, 0.1, 1, 10, 100mM BDE47 for 48h. Cell viability was detected by trypan blue exclusion; ultrastructure by electron microscopy; cell cycle, mitochondrial membrane motential (MMP) reactive oxygen species (ROS) by flow cytometry; ATP production by luminometer; Atp5b, Uqcrc1, Bcl 2 level by WB. To explore whether the decreased mitochondrial proteins play an important role in apoptosis, MMP and apoptosis were detected after Atp5b or Uqcrc1 knockdown in GC2. Results showed BDE47 reduced cell viability, caused condensation of nuclear and vacuolated mitochondria, decreased MMP and ATP, induced ROS, cell cycle arrest at S and G2/M phase, reduced Atp5b, Uqcrc1, Bcl 2 in GC2. Knockdown of Atp5b or Uqcrc1 decreased MMP, induced apoptosis in GC2. Results suggested that BDE47 reduced cell viability, injured mitochondria in spermatocytes probably by decreasing mitochondrial protein Atp5b and Uqcrc1.
Environmental toxicology and pharmacology