( ( ( Successful transformation ) ( of ( the gene ) ) ) ( ( was ( confirmed ( by ( Southern ( blot ( hybridization ( and ( polymerase ( chain reaction ) ) ) ) ) ) ) ) ) . ) )
(ROOT (S (NP (NP (JJ Successful) (NN transformation)) (PP (IN of) (NP (DT the) (NN gene)))) (VP (VBD was) (VP (VBN confirmed) (PP (IN by) (NP (JJ Southern) (NN blot) (NN hybridization) (CC and) (NN polymerase) (NN chain) (NN reaction))))) (. .)))
Successful transformation of the gene was confirmed by Southern blot hybridization and polymerase chain reaction.
(ROOT (S (NP (DT The) (NN polymerase) (NN chain) (NN reaction)) (VP (VBZ is) (NP (NP (DT a) (NN way)) (PP (IN of) (S (VP (VBG making) (NP (NP (NNS copies)) (PP (IN of) (NP (DT a) (NN gene))))))))) (. .)))