( ( ( ( ( Integral ( transmembrane proteins ) ) ( were ( isolated ( from ( ( plasma membranes ) ( of ( the cells ) ) ) ) ) ) ) and ) ( ( Cx ( 43 antibody ) ) ( was ( used ( for ( Western ( blot analysis ) ) ) ) ) ) ) . )
(ROOT (S (S (NP (JJ Integral) (NN transmembrane) (NNS proteins)) (VP (VBD were) (VP (VBN isolated) (PP (IN from) (NP (NP (NN plasma) (NNS membranes)) (PP (IN of) (NP (DT the) (NNS cells)))))))) (CC and) (S (NP (JJ Cx) (CD 43) (NN antibody)) (VP (VBD was) (VP (VBN used) (PP (IN for) (NP (JJ Western) (NN blot) (NN analysis)))))) (. .)))
Integral transmembrane proteins were isolated from plasma membranes of the cells and Cx 43 antibody was used for Western blot analysis.
(ROOT (S (NP (JJ Transmembrane) (NNS proteins)) (VP (VBP span) (NP (DT the) (JJ entire) (NN plasma) (NN membrane))) (. .)))