( ( ( ( ( Sample ( Preparation ( Peripheral blood ) ) ) ( -LRB- ( ( 500 ml ) -RRB- ) ) ) or ) ( ( bone marrow ) ( -LRB- ( ( 200 ml ) -RRB- ) ) ) ) ( ( was ( ( ( ( made up ) ( to ( ( 15 ( ( ml ( with ( red ( cell lysis ) ) ) ) buffer ) ) ( -LRB- ( ( ( ( ( ( 154 ( mM ( ammonium chloride ) ) ) , ) ( 10 ( mM ( potassium bicarbonate ) ) ) ) , ) ( 0.1 ( mM EDTA ) ) ) -RRB- ) ) ) ) ) and ) ( ( left ( for ( ( ( five to ) 10 ) minutes ) ) ) ( at ( room temperature ) ) ) ) ) . ) )
(ROOT (S (NP (NP (NP (NNP Sample) (NNP Preparation) (NNP Peripheral) (NN blood)) (PRN (-LRB- -LRB-) (NP (QP (CD 500) (CD ml))) (-RRB- -RRB-))) (CC or) (NP (NP (NN bone) (NN marrow)) (PRN (-LRB- -LRB-) (NP (QP (CD 200) (CD ml))) (-RRB- -RRB-)))) (VP (VBD was) (VP (VP (VBN made) (PRT (RP up)) (PP (TO to) (NP (NP (CD 15) (ADJP (JJ ml) (PP (IN with) (NP (JJ red) (NN cell) (NNS lysis)))) (NN buffer)) (PRN (-LRB- -LRB-) (NP (NP (CD 154) (NN mM) (NN ammonium) (NN chloride)) (, ,) (NP (CD 10) (NN mM) (NN potassium) (NN bicarbonate)) (, ,) (NP (CD 0.1) (NN mM) (NNP EDTA))) (-RRB- -RRB-))))) (CC and) (VP (VBN left) (PP (IN for) (NP (QP (CD five) (TO to) (CD 10)) (NNS minutes))) (PP (IN at) (NP (NN room) (NN temperature)))))) (. .)))
Sample Preparation Peripheral blood (500 ml) or bone marrow (200 ml) was made up to 15 ml with red cell lysis buffer (154 mM ammonium chloride, 10 mM potassium bicarbonate, 0.1 mM EDTA) and left for five to 10 minutes at room temperature.
(ROOT (S (PP (IN In) (NP (DT the) (NNS lungs))) (, ,) (NP (NN bicarbonate)) (VP (VBZ is) (VP (VBN transported) (PRT (RP back)) (PP (IN into) (NP (NP (DT the) (JJ red) (NN blood) (NNS cells)) (PP (IN in) (NP (NN exchange))))) (PP (IN for) (NP (NN chloride))))) (. .)))