( ( To ( determine ( ( ( ( the role ) ( of ( the ( accessory proteins ) ) ) ) and ) ( ( the role ) ( of ( ( ( specific functional ) domains ) ( of ( ( KIPl or ) CIN8 ) ) ) ) ) ) ) ) ( , ( we ( ( ( ( ( isolate or ) create ) ( ( yeast mutants ) defective ) ) ( in ( ( ( a ( specific gene ) ) or ) ( functional domain ) ) ) ) . ) ) ) )
(ROOT (S (S (VP (TO To) (VP (VB determine) (NP (NP (NP (DT the) (NN role)) (PP (IN of) (NP (DT the) (JJ accessory) (NNS proteins)))) (CC and) (NP (NP (DT the) (NN role)) (PP (IN of) (NP (NP (ADJP (JJ specific) (JJ functional)) (NNS domains)) (PP (IN of) (NP (NNP KIPl) (CC or) (NNP CIN8)))))))))) (, ,) (NP (PRP we)) (VP (VBP isolate) (CC or) (VBP create) (NP (NP (NN yeast) (NNS mutants)) (ADJP (JJ defective))) (PP (IN in) (NP (NP (DT a) (JJ specific) (NN gene)) (CC or) (NP (JJ functional) (NN domain))))) (. .)))
To determine the role of the accessory proteins and the role of specific functional domains of KIPl or CIN8, we isolate or create yeast mutants defective in a specific gene or functional domain.
(ROOT (S (NP (NP (NNS Genes)) (, ,) (CC or) (NP (NP (JJ functional) (NNS units)) (PP (IN of) (NP (NNS chromosomes)))) (, ,)) (VP (VBP determine) (NP (JJ specific) (NNS characteristics)) (PP (IN by) (S (VP (VBG coding) (PP (IN for) (NP (JJ specific) (NNS proteins))))))) (. .)))