( Methods ( ( The ( BIGH3 gene ) ) ( ( was ( ( examined ( for ( a mutation ) ) ) ( by ( ( ( the ( polymerase ( chain reaction ) ) ) and ) ( direct sequencing ) ) ) ) ) . ) ) )
(ROOT (S (ADVP (RB Methods)) (NP (DT The) (NNP BIGH3) (NN gene)) (VP (VBD was) (VP (VBN examined) (PP (IN for) (NP (DT a) (NN mutation))) (PP (IN by) (NP (NP (DT the) (NN polymerase) (NN chain) (NN reaction)) (CC and) (NP (JJ direct) (NN sequencing)))))) (. .)))
Methods The BIGH3 gene was examined for a mutation by the polymerase chain reaction and direct sequencing.
(ROOT (S (NP (DT The) (NN polymerase) (NN chain) (NN reaction)) (VP (VBZ is) (NP (NP (DT a) (NN way)) (PP (IN of) (S (VP (VBG making) (NP (NP (NNS copies)) (PP (IN of) (NP (DT a) (NN gene))))))))) (. .)))