( ( ( ( ( ( To ( identify isozymes ) ) ( , ( ( a ( crude ( protein extract ) ) ) ( is ( made ( by ( ( grinding ( animal ( or ( plant tissue ) ) ) ) ( with ( an ( extraction buffer ) ) ) ) ) ) ) ) ) ) , ) and ) ( ( ( the components ) ( of extract ) ) ( are ( separated ( according ( to ( ( their charge ) ( by ( gel electrophoresis ) ) ) ) ) ) ) ) ) . )
(ROOT (S (S (S (VP (TO To) (VP (VB identify) (NP (NNS isozymes))))) (, ,) (NP (DT a) (JJ crude) (NN protein) (NN extract)) (VP (VBZ is) (VP (VBN made) (PP (IN by) (S (VP (VBG grinding) (NP (NN animal) (CC or) (NN plant) (NN tissue)) (PP (IN with) (NP (DT an) (NN extraction) (NN buffer))))))))) (, ,) (CC and) (S (NP (NP (DT the) (NNS components)) (PP (IN of) (NP (NNP extract)))) (VP (VBP are) (VP (VBN separated) (PP (VBG according) (PP (TO to) (NP (NP (PRP$ their) (NN charge)) (PP (IN by) (NP (NN gel) (NNS electrophoresis))))))))) (. .)))
To identify isozymes, a crude protein extract is made by grinding animal or plant tissue with an extraction buffer, and the components of extract are separated according to their charge by gel electrophoresis.
(ROOT (S (NP (NN Ground) (NN tissue)) (VP (VBZ makes) (PRT (RP up)) (NP (NP (JJS most)) (PP (IN of) (NP (NP (DT the) (NN inside)) (PP (IN of) (NP (DT a) (NN plant))))))) (. .)))