Murdered nursing student Karen Buckley (pictured) died of head and neck injuries, according to her death certificate . Nursing student Karen Buckley died of head and neck injuries, it was revealed today as the man charged with her murder made his second court appearance. Her death certificate detailing the fatal injuries was signed by the 24-year-old's parents Marion, 62, and John, 61, who have spoken of their heartbreak at losing their 'cherished' daughter. Miss Buckley vanished after a night out in Glasgow's west end earlier this month, sparking a four-day police search. Her remains were uncovered at High Craigton Farm, near Milngavie, East Dunbartonshire. Former private schoolboy Alexander Pacteau, 21, appeared at Glasgow Sheriff Court today at a second private hearing where he made no plea and was remanded in custody. He was charged with the murder of the Irish student and attempting to defeat the ends of justice on April 17. Miss Buckley's death certificate was officially issued by a registrar in Glasgow on Wednesday. Her family hope her body will soon be released for burial after legal concerns are dealt with. The occupational therapy student, who studied at Glasgow Caledonian Univerity, disappeared on April 11 after telling friends she was going to the loo at the Sanctuary club. Flatmates at her home in Garnethill in the city centre raised the alarm when she failed to return from the night out. Her handbag was discovered in Dawsholm Park and the search switched to a property in Dorchester Avenue and then to High Craigton Farm, where her remains were found. Miss Buckley, originally from County Cork, moved to Scotland in February and was a first-year  student. A spokesman for the Crown Office said: 'Alexander Pacteau, 21, from Glasgow, made a second appearance at Glasgow Sheriff Court today charged with the murder of Karen Buckley, and with attempting to defeat the ends of justice. 'He made no plea or declaration and was fully committed and remanded in custody. No further court dates have been set at this time. 'It would be inappropriate to comment further at this stage in proceedings.' Her remains were uncovered at High Craigton Farm (pictured), near Milngavie, East Dunbartonshire . Miss Buckley from County Cork vanished after a night out in Glasgow, sparking a four-day police search . More than 300 people - including the 24-year-old's parents attended a memorial service in Glasgow's George Square on the day Pacteau appeared in court. Her parents also read touching messages and tributes at Glasgow Caledonian University. Mr Buckley said: 'Marian and I, with our sons Brendan, Kieran and Damian, are absolutely heartbroken. 'Karen was our only daughter, cherished by our family and loved by her friends. We will miss her terribly.' 'There are so many broken hearts for you' We told yesterday how Karen's best friend Julie Malone praised the online community for sharing pictures of Karen across Facebook and Twitter during the four-day police investigation. The vigil was organised by former Scottish Socialist politician Rosie Kane. She said: 'I don't want to be here, that's the first thing I need to say. 'It seems that everyone has been devastated but we want to support Karen's family and show them the best side of the city. We're trying to make something beautiful from that horrible, horrible grief. 'There have been some songs from Scotland and Ireland and people have brought flowers and candles. 'We also had a moment's silence to think about Karen's life, what we knew of her, about her family and to wish them the best.' Her parents, John and Marian, say they are 'absolutely heartbroken' at the death of their 'cherished' daughter . Tearful friends, family and colleagues of the popular student laid flowers and left messages of condolence . Sorry we are not currently accepting comments on this article.
Murdered student Karen Buckley vanished after night out in Glasgow .
She died of head and neck injuries, according to her death certificate .
Her parents hope Miss Buckley's body will soon be released for burial .
Alexander Pacteau, 21, appeared in court and was remanded in custody .