from msm who attended the amsterdam municipal health service sti outpatient clinic in 2000 and 2001 , randomly selected stored specimens of c. trachomatis dna  positive ( as assessed by ligase chain reaction , abbott laboratories , chicago , il , usa ) rectal samples were tested for the c. trachomatis variant by real - time pcr ( 6 ) . from 2002 to 2005 , msm with symptomatic proctitis ( i.e. , purulent discharge , rectal ulceration , bleeding , or edematous mucosa ) and msm without symptoms were included . from the san francisco region , 51 lgv  positive isolates from symptomatic msm were analyzed ( 7 ) .
the isolates were collected in medical clinics ( e.g. , ambulatory care , emergency room , screening , acute care ) from 1979 to 1985 ( table ) .
lgv was assessed at the time of collection , according to phenotypic properties observed during cell culture .
although the growth characteristics of lgv serovars can be distinguished from serovars d  k , cell culture for c. trachomatis is no longer available in most clinical settings . * in 2002 and 2003 , 45 lgv l2b variants of 109 isolates have been described in detail ( 5 ) . c. trachomatis serovar typing was performed as described previously ( 5 ) .
briefly , amplification of the ompa gene ( 1.1 kb ) was performed in a nested pcr format .
the ompa nucleotide sequences were subsequently analyzed by automated dna sequencing on an abi 310 sequencer ( pe biosystems , foster city , ca , usa ) .
the sequences obtained from c. trachomatis  infected msm in 2000 and 2001 in amsterdam and from msm in san francisco were compared to the recently identified l2b variant to determine if the strain was present earlier .
the table presents the results of this analysis . in the amsterdam c. trachomatis dna  positive rectal samples ,
lgv strains were detected by real - time pcr in 2 of 67 samples in 2000 and in 4 of 28 samples in 2001 .
sequencing showed that in all 6 lgv strain  positive samples , the l2b variant was present .
also in 2002 and 2003 , 109 l2b - positive samples of 403 c. trachomatis dna  positive rectal samples were identified , of which 45 were strain l2b , and these have been described in a previous publication ( 5 ) .
all 51 san francisco specimens ( from 51 patients ) were positive for lgv variants by real - time pcr . by sequencing variable segment 2 of the ompa gene ( vs-2 )
, we identified 15 as serovar l1 , 18 as serovar l2 prototype , and 18 as the l2b variant .
we sequenced the complete ompa gene of 5 of these 18 l2b variants that originated in san francisco ; all were identical to the recently described l2b variant circulating in amsterdam .
four nucleotide changes were found when compared to reference serovars l2 , l2a , and the variant l2  , including 1 change that encoded the previously undescribed change at amino acid 162 , aatagt ( 5 ) .
the l2b lgv variant identified as the cause of all the lgv proctitis in the recent outbreak among msm in amsterdam appears to have been circulating in amsterdam in 2000 .
moreover , we showed that this l2b variant was present in the 1980s in san francisco with exactly the same mutations in the complete ompa gene .
however , since we only sequenced the ompa gene , and although the sequence was identical in old and new l2b strains , we can not exclude the possibility that it could involve different strains of c. trachomatis that differ in other parts of the genome , although this is unlikely .
since lgv causes potentially severe infections with possibly irreversible sequelae if adequate treatment is not begun promptly , early and accurate diagnosis is essential .
sequence - based nucleic - acid tests that can discriminate between lgv serovars and less invasive c. trachomatis species can help detect cases and prevent further transmission of lgv . in conclusion , our results suggest that we are dealing with the same lgv variant > 25 years later , and the current lgv outbreak in industrialized countries has most likely been a slowly evolving epidemic with an organism that has gone unnoticed in the community for many years and is now being detected by new technologies .
the numbers detected in 2005 in amsterdam suggest that a considerable reservoir exists , which emphasizes the need for ongoing public health awareness .
 we traced the chlamydia trachomatis l2b variant in amsterdam and san francisco . 
 all recent lymphogranuloma venereum cases in amsterdam were caused by the l2b variant . 
 this variant was also present in the 1980s in san francisco . 
 thus , the current " outbreak " is most likely a slowly evolving epidemic . 