a. azurea was proposed by omura et al . in 1983   and later on emended by henssen et al . in 1987   and was isolated from soil that produced two water soluble antibiotics , namely , azureomycin a and b. a. azurea strain dsm 43854 was obtained from the open collection of the german collection of microorganisms and cell cultures ( dsmz ) , braunschweig , germany .
the strain a. azurea dsm 43854 is a gram - positive bacteria and contains aerial mycelium with white to blue color .
genomic dna was extracted from a 48  hour old culture using zr fungal / bacterial dna miniprep as per manufacturer 's instructions .
sequencing resulted in 22,170,928 paired - end reads ( insert size of 350  bp ) of 101  bp length .
a total of 21,919,041 high - quality reads with approximately 480   coverage were assembled using clcbio wb5.5 ( http://www.clcbio.com ) ( word size 55 and bubble size 60 ) to obtain 154 contigs ( n50 , 127,182  bp ) .
the functional annotation was carried out by rast ( rapid annotation using subsystem technology ) , trna was predicted by trnascan - se-1.23   and rrna genes by rnammer 1.2 .
the genome contains 3 rrna genes ( 5s23s16s ) and 58 aminoacyl - trna synthetase genes .
the draft genome of a. azurea consists 154 contigs of 9,223,451  bp with an average g  +  c content of 69.0% .
a total of 8603 coding regions ( 4406 from a positive strand and 4197 from a negative strand ) were found in the genome of which 5396 ( 63% ) could be functionally annotated .
the genome coding density is 89% with an average gene length of 948  bp .
the annotated genome has 117 genes involved in virulence , disease and defense including 61 genes for resistance to antibiotics and toxic compounds such as tetracycline , vancomycin , arsenic and beta - lactamase ( fig .
the genome comprises of 55 genes for sulfur metabolism and 47 genes for phosphorus metabolism .
the homology searches revealed that the pks gene clusters are supposed to be responsible for the biosynthesis of naptomycin , macbecin , rifamycin , mitomycin , maduropeptin enediyne , neocarzinostatin enediyne , c-1027 enediyne , calicheamicin enediyne , landomycin , simocyclinone , medermycin , granaticin , polyketomycin , teicoplanin , balhimycin , vancomycin , staurosporine , rubradirin and complestatin .
the functional comparison of genome sequences available on the rast server revealed the closest neighbors of a. azurea as actinosynnema mirum dsm 43827 ( score 536 ) followed by streptomyces sp .
aa4 ( score 432 ) , saccharomonospora viridis dsm 43017 ( score 376 ) and rhodococcus jostii rha1 ( score 357 ) .
a. azurea was proposed by omura et al . in 1983   and later on emended by henssen et al . in 1987   and was isolated from soil that produced two water soluble antibiotics , namely , azureomycin a and b. a. azurea strain dsm 43854 was obtained from the open collection of the german collection of microorganisms and cell cultures ( dsmz ) , braunschweig , germany .
the strain a. azurea dsm 43854 is a gram - positive bacteria and contains aerial mycelium with white to blue color .
genomic dna was extracted from a 48  hour old culture using zr fungal / bacterial dna miniprep as per manufacturer 's instructions .
sequencing resulted in 22,170,928 paired - end reads ( insert size of 350  bp ) of 101  bp length .
a total of 21,919,041 high - quality reads with approximately 480   coverage were assembled using clcbio wb5.5 ( http://www.clcbio.com ) ( word size 55 and bubble size 60 ) to obtain 154 contigs ( n50 , 127,182  bp ) .
the functional annotation was carried out by rast ( rapid annotation using subsystem technology ) , trna was predicted by trnascan - se-1.23   and rrna genes by rnammer 1.2 .
the genome contains 3 rrna genes ( 5s23s16s ) and 58 aminoacyl - trna synthetase genes .
the draft genome of a. azurea consists 154 contigs of 9,223,451  bp with an average g  +  c content of 69.0% .
a total of 8603 coding regions ( 4406 from a positive strand and 4197 from a negative strand ) were found in the genome of which 5396 ( 63% ) could be functionally annotated .
the genome coding density is 89% with an average gene length of 948  bp .
the annotated genome has 117 genes involved in virulence , disease and defense including 61 genes for resistance to antibiotics and toxic compounds such as tetracycline , vancomycin , arsenic and beta - lactamase ( fig .
the genome comprises of 55 genes for sulfur metabolism and 47 genes for phosphorus metabolism .
the homology searches revealed that the pks gene clusters are supposed to be responsible for the biosynthesis of naptomycin , macbecin , rifamycin , mitomycin , maduropeptin enediyne , neocarzinostatin enediyne , c-1027 enediyne , calicheamicin enediyne , landomycin , simocyclinone , medermycin , granaticin , polyketomycin , teicoplanin , balhimycin , vancomycin , staurosporine , rubradirin and complestatin .
the functional comparison of genome sequences available on the rast server revealed the closest neighbors of a. azurea as actinosynnema mirum dsm 43827 ( score 536 ) followed by streptomyces sp .
aa4 ( score 432 ) , saccharomonospora viridis dsm 43017 ( score 376 ) and rhodococcus jostii rha1 ( score 357 ) .
the a. azurea dsm 43854 whole genome shot gun ( wgs ) project has been deposited at ddbj / embl / genbank under the project accession number anmg00000000 of the project ( 01 ) and has the accession numbers anmg01000000 and consists of sequences anmg01000001anmg01000154 .
the authors declare that there is no conflict of interest on any work published in this paper .
 we report the 9.2  mb genome of the azureomycin a and b antibiotic producing strain amycolatopsis azurea isolated from a japanese soil sample . 
 the draft genome of strain dsm 43854 t consists of 9,223,451  bp with a g  +  c content of 69.0% and the genome contains 3 rrna genes ( 5s23s16s ) and 58 aminoacyl - trna synthetase genes . 
 the homology searches revealed that the pks gene clusters are supposed to be responsible for the biosynthesis of naptomycin , macbecin , rifamycin , mitomycin , maduropeptin enediyne , neocarzinostatin enediyne , c-1027 enediyne , calicheamicin enediyne , landomycin , simocyclinone , medermycin , granaticin , polyketomycin , teicoplanin , balhimycin , vancomycin , staurosporine , rubradirin and complestatin . 