polycystic ovary syndrome ( pcos ) , also known as stein - leventhal syndrome , is a common endocrine disorder that occurs in 510% of women of reproductive age .
according to the 2003 rotterdam criteria , it is usually defined by presence of at least 2 out of the following 3 features : i ) oligoovulation or anovulation , ii ) clinical and/or biochemical signs of hyperandrogenism ( presence of at least 2 signs ) , or iii ) polycystic ovaries ( ovaries with many small cysts no larger than 810 mm ; they occur in 1520% of women ) .
pcos has significant and various clinical implications , including reproductive , endocrine , and metabolic disorders such as hyperandrogenism and obesity .
obesity , especially abdominal obesity , is an independent factor aggravating pcos endocrine abnormalities , as subcutaneous abdominal adipose tissues and the liver tissues contribute to extragonadal aromatization .
as individuals become obese and their adipocytes enlarge , adipose tissue undergoes molecular and cellular changes affecting systemic metabolism . fasting body ffa and glycerol discharge from adipocytes
 refers to a condition characterized by an increase in levels of various inflammatory markers such as c - reactive protein ( crp ) , fibrinogen , tumor necrosis factor- ( tnf- ) , interleukin-1 ( il-1 ) , interleukin-6 ( il-6 ) , and neutrophil - to - lymphocyte ratio ( n / l ratio ) .
recognition of the impact of inflammation on atheromatous plaque generation and rupture leads to interest in novel inflammatory markers and determining the best assay to define the relevant cardiovascular diseases ( cvds ) .
respect , cellular immune activation can easily and sensitively be monitored by measurement of neopterin levels in biological fluids .
biosynthetically derived from guanosine triphosphate , neopterin [ 2-amino-4-hydroxy-6-(d - erythro-1,2,3-trihydroxypropyl)-pteridin ] belongs to the class of pteridines .
patients with pcos are considered to be at high risk for cardiovascular diseases because of hyperandrogenism , insulin resistance , glucose intolerance , type ii diabetes , and obesity which might accompany pcos .
however , there is no study in the literature reporting a direct relationship between increased / early cardiovascular mortality or morbidity and pcos .
the purpose of this study was to make a comparison between obese and non - obese patients with pcos and control subjects with regard to concentrations of inflammatory markers ( neopterin , il-6 , tnf- , n / l ratio , and crp ) and vitamin b12 , which might pose a risk for atherosclerosis in patients with pcos , and to investigate the correlation between the examined concentrations and the hormonal anthropometric and cardiometabolic parameters .
this study was conducted in the obstetrics and gynecology clinic of dicle university and approved by the local ethics committee of the university .
a total of 30 patients between 18 and 33 years of age ( mean 26.24.0 years ) who presented to dicle university faculty of medicine clinic of obstetrics and gynecology between june 2013 and july 2014 and were diagnosed with pcos based on their clinical and endocrinological data were included in this study . according to the revised rotterdam diagnostic criteria ,
pcos was defined by the presence of at least 2 out of the following 3 features : oligo - anovulation ( menstrual cycle length > 45 days or less than 6 menstrual cycles a year ) , clinical or biochemical hyperandrogenism ( ferriman - gallowey ( fg ) score > 8 or elevated serum testosterone levels ) , and polycystic ovaries on transvaginal usg ( tvusg ) ( enlarged ovaries with increased stromal volume and more than 10 follicles which measure 28 mm in diameter and localize along the periphery of the ovary in a way to form  a pearl necklace appearance .
thyroid functions , luteinizing hormone / follicle - stimulating hormone ( lh / fsh ) , prolactin , dehydroepiandrosterone sulfate ( dheas ) , 17-hydroxyprogesterone ( 17-ohp ) , androstenedione , and total and free testosterone were checked in patients with pcos and control subjects , and those with thyroid diseases , hyperprolactinemia , cushing s disease , or congenital adrenal hyperplasia and those who were administered agents such as hormonal agents , ovulation inducing agents , glucocorticoids , anti - androgens , or anti - hypertensives over the last 6 months prior to the study were excluded from the study .
fifteen patients with body mass index ( bmi ) > 25 and 15 patients with bmi < 25 were included in the pcos groups and 15 healthy control subjects with bmi > 25 and 15 healthy control subjects with bmi < 25 who were between 17 and 35 years of age ( mean 27.64.3 years ) were included in the control groups . they were all clinically normal normo - ovulatory women who were selected for the study based solely on their bmi .
all the participants , who were given detailed information about the study , gave their informed consent .
bmi was calculated in all participants using the following formula : weight ( kg)/height ( m ) .
thus , in the who and nih guidelines for asians , overweight is a bmi between 23 and 24.9 kg / m and obesity a bmi > 25 kg / m .
waist and hip circumferences were measured , and those with waist - to - hip ratio ( whg ) > 0.85 were regarded as android obese .
waist circumference was measured at the minimum circumference between the iliac crest and rib cage , and hip circumference was measured at the maximum circumference between waist and thighs .
nine anatomic regions were assessed on a 4-point scale where 0 meant no growth of terminal hair and 4 meant maximum hair growth .
scores below 8 were regarded as normal and scores between 8 and 36 were regarded as pathological .
in addition to family history of metabolic disorders , a detailed history including menstrual cycle pattern , temporal or situated in the temples of the head profile , severity of unwanted hair growth , and drug intake was taken at the time of enrollment .
clinical examination included measurement of body weight ( kg ) , height ( cm ) , and waist and hip circumferences ( cm ) as well as fg scoring .
fg scoring was done by a single observer , and scores above 8 were taken as significant .
a single observer performed trans - abdominal or transvaginal ultrasonography to reveal findings of polycystic ovarian morphology ( presence of at least 10 follicles around the ovary that measure 28 mm in size , with increased ovarian volume and/or echogenic ovarian stroma ) .
blood samples were taken from the patients at early follicular phase ( between the third and the fifth days of the spontaneous or gestagen - induced menstrual cycle ) .
venous blood was taken from the forearm between 08.0010.00 am after 8 h of fasting .
blood samples were immediately centrifuged , and sera were kept at 80c until laboratory testing .
serum neopterine , tnf- , and il 6 levels were determined using enzyme - linked immunosorbent assay method ( sunred biotechnology company , shanghai , china ) according to the manufacturer s protocols .
serum triglyceride ( tag ) and total cholesterol ( total - c ) levels were measured using an architect c 16000 autoanalyzer ( abbott laboratories , abbott park , il , usa ) .
vitamin b12 , follicle - stimulating hormone ( fsh ) , luteinizing hormone ( lh ) , and estradiol 2 ( e2 ) levels were determined by electrochemiluminescence immunoassay using a cobas e 601 analyzer ( roche diagnostics , mannheim , germany ) .
serum - free testosterone ( f.test . ) levels were analyzed by means of radioimmunoassay ( ria ) using a ria testosterone commercial kit ( immunotech , beckman coulter ) .
serum crp levels were measured using immunochemistry system nephelometric method ( image 800 , beckman coulter , usa ) . for n / l ratio measurements ,
complete blood counts of patients with pcos and control subjects were studied using an automated hematology analyzer ( cell - dyn ruby - abbott diagnostics , usa ) .
statistical package for the social science ( spss 10.0 ) was used for data analyses .
t test and nonparametric mann - whitney u test were used for comparison of clinical and biochemical data between the groups . whether intra - group variables demonstrated a normal distribution or not was determined by kolmogorov - smirnov test .
spearman s correlation analysis was used for investigation of correlation between the values . a p value
in addition to family history of metabolic disorders , a detailed history including menstrual cycle pattern , temporal or situated in the temples of the head profile , severity of unwanted hair growth , and drug intake was taken at the time of enrollment .
clinical examination included measurement of body weight ( kg ) , height ( cm ) , and waist and hip circumferences ( cm ) as well as fg scoring .
fg scoring was done by a single observer , and scores above 8 were taken as significant . a single observer performed trans - abdominal or transvaginal ultrasonography to reveal findings of polycystic ovarian morphology ( presence of at least 10 follicles around the ovary that measure 28 mm in size , with increased ovarian volume and/or echogenic ovarian stroma ) .
blood samples were taken from the patients at early follicular phase ( between the third and the fifth days of the spontaneous or gestagen - induced menstrual cycle ) .
venous blood was taken from the forearm between 08.0010.00 am after 8 h of fasting .
blood samples were immediately centrifuged , and sera were kept at 80c until laboratory testing .
serum neopterine , tnf- , and il 6 levels were determined using enzyme - linked immunosorbent assay method ( sunred biotechnology company , shanghai , china ) according to the manufacturer s protocols .
serum triglyceride ( tag ) and total cholesterol ( total - c ) levels were measured using an architect c 16000 autoanalyzer ( abbott laboratories , abbott park , il , usa ) .
vitamin b12 , follicle - stimulating hormone ( fsh ) , luteinizing hormone ( lh ) , and estradiol 2 ( e2 ) levels were determined by electrochemiluminescence immunoassay using a cobas e 601 analyzer ( roche diagnostics , mannheim , germany ) .
serum - free testosterone ( f.test . ) levels were analyzed by means of radioimmunoassay ( ria ) using a ria testosterone commercial kit ( immunotech , beckman coulter ) .
serum crp levels were measured using immunochemistry system nephelometric method ( image 800 , beckman coulter , usa ) . for n / l ratio measurements , complete blood counts of patients with pcos and control subjects
were studied using an automated hematology analyzer ( cell - dyn ruby - abbott diagnostics , usa ) .
statistical package for the social science ( spss 10.0 ) was used for data analyses .
t test and nonparametric mann - whitney u test were used for comparison of clinical and biochemical data between the groups . whether intra - group variables demonstrated a normal distribution or not was determined by kolmogorov - smirnov test .
in this study , no statistically significant difference was found between patients with pcos and control subjects in age and bmi ( p 0.395 , p 0.760 ) .
whr and fg scores were significantly higher in patients with pcos compared to control subjects ( p 0.001 and p 0.000 ) . fasting blood glucose levels and tag were significantly higher in patients with pcos compared to control subjects ( p 0.001 , p 0.014 ) .
lh / fsh ratio and f.test were significantly higher in patients with pcos compared to control subjects ( p 0.002 , p 0.014 ) ( table 1 ) . in this study , no statistically significant difference was found between patients with pcos and control subjects in neopterin , il-6 , tnf- and crp levels . however , n / l ratio levels were significantly higher ( p : 0.045 ) and vitamin b12 levels were significantly lower ( p : 0.033 ) in patients with pcos compared to control subjects ( table 1 , figure 1 ) .
whr and fg scores , which were used for rating of hirsutism , were significantly higher in both obese and non - obese patients with pcos compared to control subjects ( p 0.000 and p 0.000 ; p 0.000 and p 0.024 , respectively ) .
the comparison between obese patients with pcos and obese control subjects showed that fasting blood glucose levels were significantly higher in obese patients with pcos ( p : 0.001 ) .
total - c and tag levels and lh / fsh ratio were significantly higher in the non - obese group with pcos compared to control subjects ( p 0.001 , p 0.007 and p 0.024 , respectively ) .
crp levels were significantly higher in obese patients with pcos compared to control subjects ( p 0.007 ) . in the non - obese groups , il-6 levels were significantly higher in patients with pcos compared to control subjects ( p 0.021 ) ( table 2 , figure 1 ) .
whr were significantly higher in obese patients with pcos compared to non - obese patients with pcos ( p 0.047 ) .
triglyceride and crp levels were significantly higher in obese patients with pcos compared to non - obese patients with pcos ( p 0.029 and 0.018 , respectively ) ( table 3 ) .
n / l ratio levels were found to be significantly higher in non - obese patients with pcos compared to obese control subjects ( p : 0.042 ) .
in addition , il-6 levels were significantly higher in non - obese patients with pcos compared to obese patients with pcos .
furthermore , crp levels were significantly higher in obese patients with pcos compared to non - obese patients with pcos ( p : 0.018 ) ( table 3 ) . in addition , vitamin b12 was negatively correlated with lh / fsh ratio , and no correlation was found between vitamin b12 and the inflammatory markers ( table 4 ) .
the correlation analysis was performed in the present study demonstrated that tag and total - c were positively correlated with crp .
we found that neopterin , il-6 , tnf- , and n / l ratio were positively correlated with the pcos parameters .
furthermore , we demonstrated that neopterin and il-6 were positively correlated with lh / fsh ratio , a pcos biomarker ( r : 0.443 * p 0.014 and r 0.373 * p 0.042 , respectively ) .
neopterin and tnf- , on the other hand , was positively correlated with e2 ( r 0.612 * * p 0.000 and r 0.431 * p 0.001 , respectively ) .
in addition , vitamin b12 levels were found to be significantly lower in patients with pcos .
the correlation analysis revealed a negative correlation between vitamin b12 and lh / fsh ratio ( figure 2 ) .
the correlation analysis that was performed between crp , bmi , whr , tag , and total - c levels found a positive correlation between crp levels and bmi , whr , tag , and total - c levels ( table 4 , figure 2 ) .
several studies reported an increased risk for cardiovascular diseases in patients with pcos ; however , the reason for this increased cardiovascular risk has not been clarified yet .
chronic low - grade inflammation , such as is seen in individuals with an excess of visceral / ectopic fat , plays an important role in cardiovascular disorders , in addition to atherosclerosis , which is strongly influenced by the inflammatory components of visceral obesity .
compared with that of lean individuals , adipose tissue in obese persons shows higher expression of proinflammatory proteins , including tnf- , interleukin 6 ( il-6 ) , transforming growth factor 1 , monocyte chemotactic protein 1 , inducible nitric oxide synthase , procoagulant proteins such as tissue factor , and factor vii plasminogen activator inhibitor type 1 .
the purpose of this study was to investigate inflammatory markers in pcos as well as the impact of obesity on the inflammatory markers .
n / l ratio levels were significantly higher in patients with pcos compared to control subjects .
n / l ratio levels were found to be significantly higher in non - obese patients with pcos compared to obese control subjects ( p 0.042 ) .
differences between the neopterin , crp , il-6 , and tnf- levels were not statistically significant in patients with pcos compared to control subjects .
this result might have been caused by the limited number of participants included in this study .
we used ultrasound to assess central obesity ; however , to evaluate central obesity magnetic resonance ( mr ) , computed tomography ( ct ) , and dual energy x - ray absorptiometry ( dexa ) may also used .
patients as well as control subjects were divided into 2 groups as those with bmi > 25 and those with bmi < 25 , and it was found that only 1 inflammatory marker  crp 
the increased inflammation in patients with pcos has not been clearly explained yet , and it is still unknown whether it is caused by pcos itself or the accompanying obesity .
a meta - analysis reported increased wbc and crp levels in patients with pcos , and the increase was found to be independent of obesity .
however , the meta - analysis had the limitation of inadequate bmi matching concerning the studies included .
many researchers found increased crp levels in obese and non - obese patients with pcos compared to bmi - matched control subjects .
in addition , crp levels were reported to be significantly higher in a pcos group with bmi > 25
however , there was no significant difference in crp levels between non - obese insulin - resistant pcos group and non - obese control subjects .
the results of the present study were in agreement with those of the abovementioned studies , showing that crp might be affected by obesity .
studies showed that n / l ratio correlated with crp in several diseases with chronic inflammation such as hypertension and diabetes mellitus . in this study
crp was positively correlated with bmi , whr , tag , and total - c .
studied leukocytes as an inflammatory marker , and found that neither patients with pcos nor control subjects had leukocytosis ; however , leukocyte count was significantly higher in patients with pcos .
these results were interpreted as a potential explanation for the increased cardiovascular risk in patients with pcos . in the present study ,
n / l ratio levels were found to be significantly higher in patients with pcos compared to control subjects . in addition , crp levels were significantly higher in obese patients with pcos ( p 0.018 ) .
it can be inferred from these results that increased inflammation is responsible for the increased risk for cardiovascular diseases and niddm in patients with pcos . as to the comparison between non - obese patients with pcos and obese control subjects , crp levels were higher in patients with pcos but the difference was not statistically significant .
we also found that crp was positively correlated with bmi and therefore was affected by obesity .
however , it could increase independent of obesity in patients with pcos but the increase would be more significant in obese patients compared to non - obese patients .
high serum neopterin levels in patients with atherosclerotic diseases revealed that immunological mechanisms might be responsible for development of atherosclerosis and related complications .
it was reported that neopterin , a key molecule taking part in organization of immune response , was released from active macrophages of atheromatous plaque and could be helpful for cardiovascular risk stratification . in this study ,
neopterin levels were higher in patients with pcos , but the difference was not statistically significant , perhaps due to the limited number of cases in our study .
this finding suggests that macrophage activation occurs in women with pcos , as has been reported in cardiovascular disease .
found that increased concentrations of scrp , neopterin , and wbc might be a contributory factor in increased risk for cardiovascular diseases in patients with pcos .
in addition to the widely known risk factors for cvds ( such as hypertension , dyslipidemia , insulin resistance , and obesity ) , pcos comes to the foreground as a new risk factor for cvds .
it was shown for the first time by this study that patients with pcos had significantly higher serum neopterin levels compared to healthy women with regular menstrual cycles . in the present study , n / l ratio levels were significantly higher in patients with pcos compared to control subjects . increased concentrations of n / l ratio might be a contributory factor in increased risk for cardiovascular diseases in patients with pcos .
subclinical inflammation is considered to be a cause of atherogenesis , and il-6 and tnf- are also regarded as classic cardiovascular risk factors .
the most widely known and perhaps the most important systemic effect of il-6 is the effect on acute - phase proteins , particularly c - reactive protein ( crp ) .
pcos is a proinflammatory disorder , and several studies on pcos demonstrated increased levels of circulatory inflammatory markers such as plasminogen activator inhibitor-1 , il-18 , tnf- , il-6 , and hscrp .
gonzalez et al . found that serum tnf- and il-6 levels were increased in patients with pcos and that the excess of adipose tissue was the most probable source . in this study ,
il-6 levels were significantly higher in non - obese patients with pcos compared to obese patients with pcos .
it was demonstrated that il-6 is positively correlated with lh / fsh ratio , a pcos biomarker .
our study supports the studies in the literature showing that inflammation markers increase in pcos , independent of obesity .
it was shown that inflammatory markers that indicate low - grade chronic inflammation such as crp and il-6 were important predictors of niddm development .
crp levels were found to be elevated in patients with pcos , and this finding was considered to be supportive of the hypothesis that elevated crp levels triggered chronic inflammation , leading to an increased risk for niddm and cvds in pcos .
kurt et al . found significantly increased levels of crp , leukocytes , and neutrophils , which are inflammatory markers , and the increase was reported to be independent of obesity .
although closely related to hyperglycemia , tnf- is produced independently of obesity . in the present study , when obese patients with pcos were compared to the non - obese patients with pcos , we found that il-6 levels were significantly higher in non - obese patients ( p 0.021 ) .
in addition , il-6 and neopterin were positively correlated with lh / fsh ratio , a pcos biomarker , and tnf- was negatively correlated with whr .
therefore , we conclude that il-6 and tnf- are positively correlated with pcos biomarkers , independent of obesity . in the present study ,
whr was found to be significantly higher in patients with pcos ( both obese and non - obese ) compared to control subjects , which led to the consideration that central body fat deposition occurred in a pathophysiological mechanism that was independent of obesity in patients with pcos .
hosseinzadeh and rocha - gonzalez found that cyanocobalamin may have anti - inflammatory effect ( 38 , 39 ) .
found significantly lower serum vitamin b12 levels in obese patients with pcos compared to obese control subjects . in the present study , as well , serum vitamin b12 levels were significantly lower in patients with pcos ( p 0.033 ) .
however , unlike the abovementioned study , no significant difference was found between obese and non - obese patients with pcos .
therefore , b12 can say that independently of obesity decreased in pcos . in the present study
inflammatory activity is increased in patients with pcos , can lead to an increased risk for atherosclerosis , and this increase is not caused by obesity but rather by the polycystic ovary syndrome itself .
 backgroundwe aimed to measure the levels of inflammatory markers and neopterin in obese and non - obese patients with pcos by using 2 separate control groups with matching body mass index ( bmi).material / methodsa total of 60 women of reproductive age with ( n=30 ) and without ( n=30 ) pcos were included in this study . based on their bmi , 
 patients with pcos were divided into 2 groups as obese ( n=15 ) and non - obese ( n=15 ) pcos groups . 
 in addition , 2 bmi - matched control groups were formed . 
 neopterin , tumor necrosis factor- ( tnf- ) , interleukin-6 ( il-6 ) , c - reactive protein ( crp ) , neutrophil - to - lymphocyte ratio ( n / l ratio ) , and vitamin b12 were assessed by complete blood count.resultsno significant difference was found between patients with pcos and control subjects in neopterin , il-6 , tnf- , and crp levels . however , n / l ratio levels were significantly higher ( p 0.045 ) and vitamin b12 levels were significantly lower ( p 0.033 ) in patients with pcos compared to control subjects.no statistically significant difference was found between obese and non - obese patients with pcos and control subjects in neopterin , il-6 , tnf- , and n / l ratio levels . however , crp levels were significantly higher in obese patients with pcos compared to obese control subjects ( p 0.007).conclusionsit can be concluded that inflammatory activity is increased in patients with pcos , can lead to an increased risk for atherosclerosis , and this increase is not caused by obesity but rather by the polycystic ovary syndrome itself . 
 however , studies with larger sample sizes are needed in this area . 