aki remains an immense clinical problem that is associated with high morbidity and mortality rates and affects a growing number of hospitalized patients .
there is not only significant physical injury to millions of individual patients but also a large economic impact to society as evidenced by increased length of hospital stay and higher costs . to put the economic burden into perspective
, aki is estimated to cost the national health service ( nhs ) in the uk ( excluding costs in the community ) between  434 million and  620 million per year ( nhs kidney care estimate ) because of prolonged hospitalizations and short- and long - term morbidities . in 2005 ,
chertow and colleagues   estimated that the annual costs for hospital - acquired aki were approximately $ 10 billion in the us .
aki is the descriptive term for the clinical condition that occurs when the renal excretory function is critically and acutely decreased to a point at which the body accumulates  uremic  waste products and becomes unable to maintain electrolyte , acid - base , and water balance . in clinical terms
, aki is measured as an increase in serum creatinine , a biomarker that is universally used but that has considerable diagnostic limitations given its variability , dependence on body muscle mass , and delay in accumulation .
aki is a clinical entity and has various etiologies that include interstitial nephritis , rapidly progressive glomerulonephritis , obstructive nephropathy , and renovascular complications but is frequently the consequence of ischemic and toxic insults and also occurs commonly in the setting of sepsis . in sepsis ,
the circulation is hyperdynamic and blood flow is altered , though not necessarily in the ischemic range , while the glomerular filtration rate drops rapidly .
the pathophysiology of sepsis - associated aki is very complex and involves an intricate interplay of inflammation , oxidative stress , microvascular dysfunction , and further amplification of injury by the secretion of chemokines and cytokines by tubular cells . in laboratory science , the pathophysiology and therapy of aki
are most commonly investigated in animals with ischemia / reperfusion injury induced by clamping of both renal pedicles .
other less commonly used models include toxic injury models ( for example , cisplatinum and folic acid ) and a sepsis model using cecal ligation and puncture .
accordingly , most knowledge regarding the pathophysiology of aki has been derived from preclinical studies in rats and mice with ischemia / reperfusion - induced aki .
one of the major obstacles that has hampered therapeutic progress in aki is the fact that effective interventions in animals were obtained in otherwise healthy animals but that most patients who develop aki present with significant co - morbidities such as older age , underlying chronic kidney disease ( ckd ) , and diabetes  conditions that are not considered in animals .
in addition , aki in most patients occurs as a syndrome of multiple coexisting etiologies , including ischemia , toxicity , and functional impairment , whereas animal models used for the study of its pathophysiology and therapy are generally monocausal and simplistic and often exhibit species - specific characteristics .
aki is the clinical endpoint of a number of processes resulting in a decrease of the glomerular filtration rate , a measure of global renal function .
important components of the injury process include apoptosis , necrosis , reactive oxygen species , and micro - vessel damage causing local ischemia , endothelial dysfunction , leaks , and inflammation ( see figure 1 ) .
several stages of aki have been delineated : initiation , extension , maintenance , and recovery phases ; however , in the clinical situation , these are not clear - cut and often appear overlapping .
apoptosis and , to a lesser extent , necrosis of renal cells are the main forms of cell death caused by injury .
however , recently identified new pathways of programmed cell death summarized under the term  necroptosis  also contribute to a variable degree to tissue damage .
necroptosis has been recognized as a regulated process under ischemic conditions with at least three pathways involved : receptor - interacting protein kinase 3 ( rip3)-dependent necroptosis , cyclophilin d - dependent pathways , and a pathway involving the poly ( adp - ribose ) polymerase - calpain axis .
recently developed approaches for translational profiling , based on transgenic animal models , facilitate a detailed approach to the identification of injury - induced changes in gene expression patterns .
these data show that a wide network of genes is activated at 24 hours after injury and is characterized by strong expression of anti - apoptotic and  anti - necrotic  pathways as well as the upregulation of genes involved in cell movement and formation of cell junctions .
these findings confirm the clinical observation of the robust regenerative potential of the tubular epithelium .
in addition , these data show that components of the normal tubular physiology are rapidly downregulated post - injury .
acute kidney injury is most commonly caused by ischemic or toxic injury and occurs in the setting of sepsis .
components of the pathophysiology include inflammatory responses as well as tubular and vascular damage and its consequences .
it is not unusual that the kidney fully recovers its function , as measured by serum creatinine levels , even in cases in which patients become dialysis dependent for weeks .
although clinical recovery appears complete , as measured by serum creatinine , there is structural and subclinical damage , which is undetectable by current clinical methods but which enhances susceptibility to injury as well as development of progressive ckd .
tubular cells are capable of activating a number of cytoprotective mechanisms , which include the expression of heat shock proteins and cell cycle regulatory proteins such as p21 . surviving tubular cells
facilitate recovery and repair through proliferation and migration after injury , resulting in the repopulation of denuded nephron segments .
autophagy , a process of self - clearing of broken - down subcellular components that are fed into the lysosomal degradation pathway , is induced after injury and has been described as a renoprotective mechanism used by tubular cells .
protection of the kidney can be induced experimentally through ischemic preconditioning whereby a sublethal ischemic insult prevents greater damage from future ischemic episodes through a number of proposed and observed mechanisms , including nitric oxide induction , cellular kinases , heat shock proteins , and neurogenic pathways , all of which augment cellular resistance to injury .
this phenomenon was described about a century ago in the kidney in the setting of uranium - induced injury .
this experimental finding has been tested in clinical trials with some promising but preliminary results .
the histopathology of aki in humans is a largely uncharted territory because of the rarity of biopsies performed in the acute phase of injury .
acute tubular necrosis  is a misnomer given that necrosis is very localized to some small focal areas and that the cell death processes involved include apoptosis and desquamation of viable cells in addition to small focal necrotic areas .
human aki differs considerably from the ischemia / reperfusion mouse model , in which large areas of kidney cortex are essentially no longer perfused , resulting in cortical necrosis . in the human situation ,
blood flow never fully ceases and damage occurs mainly from focal mismatches of oxygen delivery by impaired microcirculation and increased demand due to cellular stress .
questions regarding the extent of damage as well as the cell types affected by damage ( that is , distal versus proximal tubular cell types ) have remained controversial . again
, this debate is based primarily on data of rodent models since human aki and particularly the deeper sections of the kidney ( including the medulla ) are rarely biopsied in the acute setting .
the few data available in the human situation paint a very different picture compared with animal models .
a large study by liao and pascual   from 1996 reported 748 cases of aki , with  acute tubular necrosis  ( 45% ) being the most frequent cause , followed by prerenal failure ( 21% ) , acute - onset chronic renal failure ( 12.7% ) , and obstructive acute renal failure ( 10% ) .
however , the total number of renal biopsies performed in this study was low ( 6.1% ) .
a study from 2008 analyzing the spanish registry of glomerulonephritis found that 16.1% of all biopsies (
n = 2,281 ) were diagnosed with acute renal failure and diagnoses were quite different , with vasculitis ( 23.3% ) , acute tubulointerstitial nephritis ( 11.3% ) , and crescentic glomerulonephritis types 1 and 2 ( 10.1% ) as leading diagnoses among aki cases . although the study was obviously limited by a strong selection bias given that most patients with aki were never biopsied in this glomerulonephritis registry , the data underscore the existence of a large knowledge gap regarding the actual histopathology of human aki .
although human data are sparse , the inflammatory component , as evidenced by infiltrating leukocytes , is rather small and most leukocytes are found within the vasa recta .
in contrast , in the mouse model of aki , inflammation is a prominent part of the injury cascade .
mouse ischemic aki is characterized by extensive infiltrates of inflammatory cells , which contribute to injury propagation by secreting pro - inflammatory cytokines and factors .
different leukocyte populations , including t and b cells , natural killer cells , t - regulatory cells , dendritic cells , and macrophages , have all been described to play various roles in the pathophysiology of and recovery from aki .
most of the studies investigating leukocyte populations have been conducted in mouse models , and this is largely because of the ready availability of immunodeficient mice as well as bone marrow transplantation models .
in contrast to the mouse model , the rat model exhibits prominent apoptosis of tubular cells as the major pathophysiological component and leukocytes play a minor role . a recent study by sutton and colleagues   demonstrates the dilemma between models impressively : in mice , p53 inhibition is detrimental by enhancing the inflammatory component ; this is in contrast to the rat model , in which p53 inhibition is protective , mainly by reducing the amount of tubular cell apoptosis .
tubular regeneration has been described as originating from surviving tubular cells that are depolarized and de - differentiated and that proliferate , migrate , and re - differentiate to repopulate denuded tubular segments .
studies have shown that tubular cells of the s3 segment exhibit a particularly robust proliferative response in response to acute injury .
these cells promptly re - enter the cell cycle and thereby contribute to the repair of injured nephron segments .
observations regarding the spontaneous contribution of stem cells from extra - renal sources , including bone marrow - derived stem cells , to kidney regeneration have been published . however , careful lineage - tracing studies have shown that their contribution is either absent or at the most minimal , thereby suggesting that intrinsic renal cells carry out the repair of the injured kidney [ 26 - 28 ] .
based on these observations , further debate regarding the mechanism of kidney repair following aki is focused on the existence and role of kidney - specific stem / progenitor cells versus regeneration carried out mainly by surviving tubular cells that do not express stem cell markers .
a leading hypothesis posits that all differentiated proximal tubule epithelia have the capacity to proliferate during repair by a mechanism of reversible dedifferentiation and self - duplication . on the other hand
, it has been postulated that stem / progenitor cells of intratubular origin are the main executioners of tubular regeneration .
the terms  stem  and  progenitor  cells are frequently used interchangeably , but , in general , the term  progenitor cells  refers to a more restricted population of cells that might not be able to differentiate into a whole array of cells . carefully designed studies using the six2-cre driver , a genetic tool for the irreversible marking of a population of cells defined by a specific marker , were carried out to lineage - trace and thus assess the contribution of intrinsic tubular cells to nephron repair .
the latter was confirmed , and no evidence for a role of extrarenal cells in this process was detected .
this study , however , was unable to exclude the existence of intratubular stem or progenitor cells .
more recent publications , using sophisticated lineage - tracing methods , have provided evidence that all tubular cells are equally able to contribute to regeneration after injury , thereby providing indirect evidence against the existence of a distinct progenitor cell population .
for a long time , the belief was that recovery from aki is complete and leads to a restitutio ad integrum .
indeed , renal function , as measured by serum creatinine levels , frequently normalizes close to or to pre - injury levels , suggesting the return of normal kidney function .
however , animal studies have shown that there is ultrastructural damage from incomplete tubular repair and persistent inflammation and deposition of extracellular matrix , together resulting in functional defects such as a decrease in urinary concentrating ability as well as salt sensitivity .
these defects contribute to continuous tissue damage by hypoxia as a result of micro - vessel rarefication , ultimately resulting in slowly progressive interstitial fibrosis and loss of function .
the notion that kidney function is not back to pre - injury baseline levels is backed by large epidemiological databases showing that aki is a trigger for subsequent declining kidney function , with a large proportion of patients progressing to advanced - stage ckd and eventually end - stage renal disease ( esrd ) .
opponents of this hypothesis posit that the most vulnerable patients would have progressed to esrd without a triggering episode of aki , since the same risk factors that predispose to aki are responsible for progression of ckd .
these risk factors include , but are not limited to , the metabolic syndrome , obesity , hypertension , diabetes , and cardiovascular diseases . by the same token , the very same risk factors also predispose to the development of aki , which blurs the lines between causality and association .
thus , it comes as no surprise that the patients with the lowest level of kidney function were the most likely to progress to esrd .
an important component of ckd development after aki is maladaptive repair carried out by fibroblasts , pericytes , and myofibroblasts , with resultant interstitial renal scar formation and progressive nephron loss .
tubular epithelial cells contribute to this process by cell cycle arrest and subsequent production of transforming growth factor - beta , thereby further fueling the process of progressive fibrosis .
however , the types of cells causing progressive fibrosis after aki are still being debated .
obvious candidates include epithelial cells , resident fibroblasts , bone marrow cells , endothelial cells , and pericytes .
several groups identified epithelial - mesenchymal transition as a major profibrotic mechanism in ckd . accordingly , proximal tubular epithelial cells transform into fibroblast - like cells and thereby initiate and promote interstitial fibrosis .
lineage - tracing studies , however , have not been able to reproduce this finding and identified pericytes as the main contributors to the fibrotic cell populations . yet the definitive origin of myofibroblasts responsible for extracellular matrix deposition
aki remains a major contributor to morbidity and mortality in hospitalized patients , and effective interventions to reduce mortality are currently not available .
the current research focus is on the epidemiology and cellular substrate of the chronic kidney damage resulting from an episode of aki .
answers regarding the cells that are responsible for the progressive interstitial fibrosis after aki have obvious therapeutic relevance . the pathophysiology of aki is highly complex and involves multiple cellular systems , including the endothelium , tubular cells , and leukocytes .
given the complex pathophysiology of aki and the various comorbidities of affected patients , effective interventions need to be directed at all major pathophysiological components of this serious complication .
given the multitude of pathophysiological components , it appears that targeting only one or a few of the principal pathomechanisms of aki would likely be ineffective , as clinical trials in the past have shown .
 acute kidney injury ( aki ) is a common clinical entity associated with high morbidity and mortality and clinical costs . 
 the pathophysiology is multifaceted and involves inflammation , tubular injury , and vascular damage . 
 recently identified components include necroptosis , a special form of cell death , and autophagy . 
 most of the pathophysiological knowledge is obtained from animal models but these do not directly reflect the reality of the clinical situation . 
 tubular cells have a remarkable capacity to regenerate , and the role of stem / progenitor cells is discussed . 
 acute kidney injury is frequently associated with chronic kidney disease , and the implications are widespread . 