due to early discharge of infants from the hospital , readmission has been increased ( 1 ) . therefore , early diagnosis of jaundice and timely actions are necessary .
the measurement of bilirubin level ( 2 ) and alpha fetoprotein ( 3 ) in cord blood have been used for this purpose . for the first time aysin nalbantoglu et al .
used alkaline phosphatase ( alp ) level 6 hours after birth as a marker for determining hemolysis and hyperbilirubinemia ( 4 ) .
alkaline phosphatase is a hydrolase enzyme and responsible for removing phosphate from many types of molecules ( 5 ) .
alkaline phosphatase is found in almost all body cells , including red blood cells ( 4 - 6 ) .
therefore , we hypothesized that it can be used as a marker for early diagnosis of hyperbilirubinemia .
this study was conducted to evaluate the alp level as a marker for early diagnosis of neonatal jaundice .
in this prospective study , between october and december 2013 , infants who were born at the babol - clinic hospital in babol , northern iran , were selected .
a total of 105 healthy term infants with gestational age between 37 and 42 weeks , weighing more than 2500 g born to healthy mothers were studied .
five milliliter cord blood was taken after birth and sent for determination of alp level .
serum alkaline phosphatase was measured with auto analyzer ( sinnowa - ds301 , china , 2013 ) .
infants who were born to mothers with diseases such as eclampsia , diabetes , bone , kidney and liver diseases , and infants who were found suffering from other diseases except jaundice were excluded from the study .
three cases were lost to the study , so 102 cases were monitored for the emergence of clinical jaundice based on clinical observation by parents or physicians up to 10 days after birth .
infants with clinical jaundice were recalled and serum bilirubin level was measured and treated based on american academy of pediatrics ( aap ) protocols . these were assigned as the treatment group and neonates without clinical jaundice were assigned as the non - jaundiced group . in treatment group , to determine the cause of hyperbilirubinemia , work - up including complete blood count , reticulocyte count , estimation of blood group in mother and neonate , peripheral blood smear , evaluation of g6pd level and coombs test , was done .
demographic information of all infants including gestational age , birth weight and apgar score was recorded .
the remaining 102 cases consisted of 50 ( 49% ) males and 52 ( 51% ) females .
ninety eight ( 96% ) infants were born by cesarean section and 4 ( 4% ) by vaginal delivery .
the mean gestational age was 38.7 weeks and the mean birth weight 3649.59 grams ( table 1 ) .
the rate of need for treatment was 9.8% ( 10 cases ) , of which 5 cases were abo incompatible , one case rh incompatible , 2 cases g6pd deficient and in 2 cases the cause of jaundice remained unknown .
hct levels and reticulocyte count were in normal range and coombs test was negative in these cases .
there was no difference between groups with regard to gestational age , birth weight and apgar scores , but the comparison of cord blood alkaline phosphatase levels revealed a significant difference between the two groups ( p value = 0.041 ) ( table 2 ) .  
values are presented as mean  sd .   values are presented as mean  sd . comparison of cord blood alkaline phosphatase levels between non - jaundiced group and jaundiced newborns in whom bilirubin level had reached  10 mg / dl , revealed a significant difference ( p value = 0.016 ) ( table 3 ) .  
values are presented as mean  sd . comparison of the non - jaundiced group with neonates who required treatment according to aap protocol ( the treatment group ) showed a significant difference in cord blood alkaline phosphatase levels ( p value = 0.040 ) ( table 4 ) .  
a comparison of the roc curves of the alkaline phosphatase levels between the non - jaundiced and treatment groups ( figure 1 ) revealed that a cord blood alkaline phosphatase level > 314
iu / l was the most suitable cutoff value for predicting severe jaundice ( that needs treatment ) .
( alp ) at the treatment group , area under the curve is 0.730 . of 60 neonates
whose cord blood alkaline phosphatase measured less than 314 iu / l , only two neonates needed treatment .
thus , the negative predictive value of cord blood alkaline phosphatase for occurrence of hyperbilirubinemia was 96.6% .
to our knowledge this is the first clinical study that examines cord blood alkaline phosphatase level as an indicator to predict severe neonatal jaundice . the results of our study indicate that measurement of cord blood alkaline phosphatase may be a predicting marker for neonatal jaundice that can exceed 10 mg / dl and necessitates treatment in the first week of life .
cord blood alkaline phosphatase level with sensitivity and specificity of 80% and 63% respectively in cutoff level > 314 iu / l predicts a need for treatment .
they found that alp levels were significantly higher in patients with hyperbilirubinemia requiring treatment , either with phototherapy or exchange transfusion ( p value 0.0001 ) ( 4 ) . in our study , there was a significant difference in the levels of cord blood alkaline phosphatase between the non - jaundiced and clinically jaundiced newborns , and it was significantly higher in patients with hyperbilirubinemia requiring treatment .
moreover , the alp levels were significantly higher in newborns whose serum bilirubin level reached a level  10 mg / dl .
one of advantages in our study was the site of sample collection , which was taken from cord blood .
in addition , the neonate may not be lost to follow - up because of early discharge .
chou et al . measured cord blood hydrogen peroxide level for prediction of neonatal hyperbilirubinemia .
the cord blood hydrogen peroxide signal level of 2500 counts/10 seconds was an appropriate cutoff for predicting severe hyperbilirubinemia with sensitivity and negative predictive value of 76.2% and 93.3% , respectively ( 7 ) .
our study showed that the alkaline phosphatase level of 314 iu / l was associated with sensitivity and negative predictive value of 80% and 96.6% , respectively .
the rate of need for treatment of jaundice in our study was 9.8% ( 10 cases ) .
of these , 5 cases were abo incompatible , one rh incompatible , 2 g6pd deficient and 2 cases were of unknown etiology . according to these findings , it seems that the alkaline phosphatase level has a higher validity in disclosing hemolytic processes .
( 8) used the cord blood bilirubin level to predict the need for phototherapy . by a cord bilirubin
cut - off level of 30 mol / l this revealed a sensitivity of 70.3% and a negative predictive value of 65.6% .
our study had a sensitivity and negative predictive value of 80% and 96.6% , respectively .
the measurement of end tidal carbon monoxide ( 9 ) at the cutoff level of 1.8 /l ( ppm ) showed a negative predictive value 97% ; our study showed a negative predictive value of 96.6% , which is comparable to this expensive and low accessible test .
the first day serum bilirubin ( 10 ) and sixth hour serum bilirubin ( 11 ) have been used for prediction of hyperbilirubinemia .
these tests require venous blood sampling ( in many countries as in ours ) , and so are not suitable for screening all neonates . in our study
found the mean level of cord blood alkaline phosphatase 159  49 iu / l ( 12 ) .
another local study by abbasian et al . in shahrood , iran showed that mean cord blood alkaline phosphatase level was 314.34  122.42
the average level of cord blood alkaline phosphatase in iranian newborns seems to be higher than in other populations .
cord blood alkaline phosphatase level is a useful indicator in predicting subsequent jaundice in healthy term newborns .
 background : management of hyperbilirubinemia remains a challenge for neonatal medicine because of the risk of neurological complications related to the toxicity of severe hyperbilirubinemia.objectives:the purpose of this study was to examine the validity of cord blood alkaline phosphatase level for predicting neonatal hyperbilirubinemia.patients and methods : between october and december 2013 a total of 102 healthy term infants born to healthy mothers were studied . 
 cord blood samples were collected for measurement of alkaline phosphatase levels immediately after birth . 
 neonates were followed - up for the emergence of jaundice . 
 newborns with clinical jaundice were recalled and serum bilirubin levels measured . 
 appropriate treatment based on serum bilirubin level was performed . 
 alkaline phosphatase levels between the non - jaundiced and jaundiced treated neonates were compared.results:the incidence of severe jaundice that required treatment among followed - up neonates was 9.8% . 
 the mean alkaline phosphatase level was 309.09  82.51 
 iu / l in the non - jaundiced group and 367.80  73.82 
 iu / l in the severely jaundiced group ( p = 0.040 ) . 
 the cutoff value of 314 iu / l was associated with sensitivity 80% and specificity 63% for predicting neonatal hyperbilirubinemia requiring treatment.conclusions:the cord blood alkaline phosphatase level can be used as a predictor of severe neonatal jaundice . 