the occurrence of screw loosening is reported to be 12.7% in single tooth restoration and 6.7% in partial fixed prostheses .
23 jemt et al.4 observed that most of the screw loosening occurred within a year and the frequency of screw loosening was reduced over time .
henry et al.5 and khraisat et al.6 stated that screw loosening is more frequent in external type implants .
in internal type connections , the stability of the prosthesis is obtained from the clamping force of the screw joint and the frictional force created by contact between the conical mating parts of the implant - abutment assembly .
because of this connection , internal type implants have more favourable stress distribution , better stability , and superior resistance to lateral load.7 however , tsuge and hagiwara8 presented no significant difference between internal and external implant - abutment connections concerning their effect on the abutment screw loosening .
theoharidou et al.9 also reported that the frequency of screw loosening in single tooth restoration was 2.7% in external type implants and 2.4% in internal type implants ; this difference was not statistically significant .
the frequency of screw loosening is reduced over time , but still occurs in spite of recent advances in the design and screw materials .
screw loosening produces discomfort for patients ; therefore , methods to prevent screw loosening are needed .
one method for preventing screw loosening that is easily applicable to clinical practice is retightening .
gradual functional loading causes sliding of the screw thread and relief of screw extension induced by preload .
gradual reduction of preload below a critical point causes screw turning . applying a greater preload on the screw in the first phase of screw loosening
siamos et al.11 reported that in order to minimize the loss of preload , it is necessary to retighten the screw 10 minutes after the first screw tightening .
however , the retightening procedure may change the shape of the abutment screw and the inner screw thread of the implant ; hence , stability during function may be affected.12 in addition , conflicting results on the effect of retightening have been reported . according to tzenakis et al.,13
if the abutment screw is repeatedly retightened , a higher preload can be obtained because of surface wear that can lower the coefficient of friction .
however , weiss et al.14 reported that repeated tightening / removal procedures decreased the reverse torque value ( rtv ) of screws .
riccardi - copped et al.15 reported that repeated tightening and removal of titanium screws caused the rtv to decrease gradually .
the effect of screw retightening remains controversial and on the timing and frequency of retightening are lacking .
the aim of this study was to assess the effect of dynamic loading and screw retightening on rtv in external and internal connection type implants .
twenty external type implants ( diameter , 4 mm ; length , 10 mm ; sola , shinhung , seoul , korea ) and 20 internal type implants ( diameter , 4 mm ; length , 10 mm ; luna , shinhung , seoul , korea ) were used ( fig .
1 ) . abutments for cementretained prostheses ( diameter , 5 mm ; gingival height , 5.5 mm ; esthetic abutment for external implant and duo abutment for internal implant , shinhung , seoul , korea ) were connected to each implant ( fig .
each implant and abutment assembly was held in a customized jig in all testing procedures . using a digital torque controller ( mgt 12 , mark-10 co. , new york , ny , usa )
after 10 minutes , the assemblies were retightened with 30 ncm torque to compensate for embedment relaxation.11 after tightening the screws twice , an initial measurement of rtv was made .
the implant / abutment assemblies were divided into 4 groups ( 10 assemblies per group ) in order to evaluate the effect of intermittent retightening : 1 ) ext - n , external type implant with no retightening ; 2 ) ext - rt , external type implant with retightening ; 3 ) int - n , internal type implant with no retightening ; 4 ) int - rt , internal type implant with retightening .
the implant / abutment assemblies were fitted to the mounting base of a universal testing machine ( electroplus e 3000 , instron , washington dc , wa , usa ) with the long axis of the implant fixed at 30 degrees relative to the vertical axis ( fig .
the testing device delivered sine curved cyclic loading between 20 and 250 n at 14 hz for 100,000 cycles . in the groups with no retightening ( ext - n and int - n ) , the rtv was measured after 100,000 cycles of loading , while rtv was measured after 3 , 10 , and 100 cycles as well as every 20,000 cycles in the retightening groups ( fig .
3 ) . for all statistical evaluations , spss version 20.0 , spss inc . ,
one - way analysis of variance ( anova ) was used for comparing rtv depending on the implant system and retightening . when the rtv was measured repeatedly after various loading cycles ,
tukey 's test was used for post hoc comparisons , and the significance level was set at =0.05 .
the initial rtvs were 27.8  1.3 ncm in external implants and 25.1  1.9 ncm in internal implants .
both implant systems showed decreased rtvs when compared with the tightening torque value of 30 ncm .
the initial rtv of external implants was significantly higher than that of internal implants ( p<.05 ) .
the rtvs of all implant / abutment assemblies were significantly reduced after cyclic loading ( p<.05 , table 1 , table 2 ) .
the rtvs of the groups without retightening were 25.4  1.2 ncm in the ext - n group and 15.8  1.8 ncm in the int - n group .
the ext - n group showed a significantly higher rtv when compared with the int - n group ( p<.05 ) .
the rtvs of the groups with retightening were 23.8  1.8 ncm in the ext - rt group and 19.9  3.3 ncm in the int - rt group .
the ext - rn group showed a significantly higher rtv than the int - rn group ( p<.05 ) . in external implants
on the other hand , retightened internal implant / abutment assemblies showed superior rtvs when compared to internal implants with no retightening ( table 1 , table 2 ) .
screw loosening is the most frequently occurring mechanical complication of implant restorations.516 abutment screw loosening has been reported in a large number of studies with an incidence ranging from 2% to 15% of abutments .
2359 screw loosening is caused by inadequate tightening torque , settling of implant components , inappropriate implant position , inadequate occlusal scheme or crown anatomy , poorly fitting frameworks , improper screw design / material , and heavy occlusal forces.171819 to overcome screw loosening and joint instability , many technical solutions have been suggested .
for example , new abutment screw designs and materials for maximizing preload,120 mechanical torque - applying instruments for optimizing tightening torque,21 precise implant components for antirotation , and internal conical connection implants with no micromotion or microgaps have been proposed . in the current study ,
the initial rtv was always smaller than the tightening torque ( 30 ncm ) in external and internal type implants .
haack et al.22 reported that most of the tightening torque is used to overcome the friction of the surface and only 10% of the tightening torque is used to generate preload .
they suggested that 75 - 80% of tightening torque remained in titanium or gold screws.22 kim et al.23 reported that the amount of remaining torque was affected by the screw material ( 77% remained in titanium alloy and 66% remained in gold alloy ) . for standardization of the results ,
the same titanium alloy was used for both internal and external abutment screws in the present study .
the results showed that the remaining torque was 90.9% in external implants and 83.3% in internal implants .
these relatively high remaining initial rtvs might have been caused by advanced milling technique and/or the screw design / material .
in the current experiments , initial rtvs and post - cyclic loading rtvs were higher in external implants than in internal implants .
this is a result of the difference in connection type between the implants . in external implants ,
joint stability is obtained by the tension of the screw , while it is achieved mainly by friction between the abutment and the implant in internal type implants.24 most of the tightening torque is used to produce preload in external implants,25 while in internal implants , tightening torque is distributed between friction with the abutment and preload on the screw .
moreover , internal implants are susceptible to the wedge effect , which arises when tightening torque and mechanical load are applied , leading to axial displacement of the abutment , so that the tensile force of the screw is lost and preload is decreased.232425 according to lee et al.24 and kim et al.,25 axial displacement of the abutment is greater in internal implants , while rtv is greater in external implants .
although screws from the same manufacturer were used for standardisation , the screws for external implants have longer threads than those of the screws used for internal implants ( fig .
1 ) . the thread - engaging surface may affect the preload in screw - tightening procedures .
to our knowledge , no internal / external implant system manufactured by the same manufacturer offers either similar or identical screw shapes owing to the different inner structures of internal / external implants .
although the 2 implant systems had different screw sizes and dimensions , the lengths of the engaging or the mating threads were similar in both.24 the upper part of the thread in the screw of the external implant was not in contact and just passed through the inner surface of the implant .
comparison of the groups with and without retightening after cyclic loading revealed no significant differences in rtvs in external implants .
however , in internal implants , retightening resulted in higher rtvs than those found in implants without retightening .
these results can be explained by the difference in connection type between the implants and the number of times the implants were retightened . in internal type implants , less settling effect occurs during tightening and loosening procedures , because tightening torque generates less preload due to dissipation of force via friction between the abutment and the implant .
therefore , a greater number of retightening procedures allows more settling effect and making an adequate surface of the screw .
cardoso et al.26 found that rtvs decreased as the number of insertion / removal cycles increased in external type implants .
in contrast , tzenakis et al.13 reported an increase of preload in internal type implants .
therefore , retightening of screws is strongly recommended for joint stability , especially in internal type implants .
the appropriate number of retightening procedures is not clear from the results of previous studies .
tzenakis et al.13 reported a gradual increase in gold screw preload from tightening for the first time to retightening 5 and 10 times in internal type implants . in the current study
it should be noted that retightening more than 5 times is not recommended for the maintenance of preload . in the present study ,
this phenomenon was observed in both external and internal type implants . according to delben et al.,27 rtvs
were maintained constantly by retightening after every 100,000 cycles of loading in external type implants . according to binon and
mchugh,28 the average daily number of mastication is about 2,700 and so 100,000 cycles corresponds to around one month . in the current study ,
the load cycle was decreased to 20,000 cycles ( corresponding to one week ) in order to infer the timing of retightening , because previous studies found no significant differences with 100,000 load cycles . in the current experiments ,
rtvs decreased only 10 rounds of cyclic loading in external and internal type implants . in our previous study,24
thus , axial displacement of the abutment screw can affect joint stability , especially in internal implants . to increase preload and secure joint stability ,
it is recommended that retightening be performed after 10 loading cycles for both external and internal implants . moreover , retightening after 1 week is also profitable for internal type implants .
this study evaluated rtv according to retightening and cyclic loading in external and internal type implants .
initial rtv and postcyclic loading rtv were higher in external implants than in internal implants . in external type implants ,
retightening did not produce a significant difference in rtv when compared with the non - retightening group after 100,000 rounds of cyclic loading . in internal type implants ,
the retightening group showed significantly increased rtvs when compared with the non - retightening group after cyclic loading .
a decrease in rtv occurred after only 10 rounds of cyclic loading , which represents the early stage of cyclic loading .
after 20,000 cycles of loading , rtvs were maintained constantly in both external and internal type implants .
 purposethe aim of this study was to evaluate the effect of cyclic loading and screw retightening on reverse torque value ( rtv ) in external and internal type implants.materials and methodscement - retained abutments were connected with 30 ncm torque to external and internal type implants . 
 experimental groups were classified according to implant connection type and retightening / loading protocol . in groups with no retightening 
 , rtv was evaluated after cyclic loading for 100,000 cycles . in groups with retightening , 
 rtv was measured after 3 , 10 , 100 cycles as well as every 20,000 cycles until 100,000 cycles of loading.resultsevery group showed decreased rtv after cyclic loading . before and after cyclic loading , external type implants had significantly higher rtvs than internal type implants . in external type implants , retightening did not affect the decrease in rtv . 
 in contrast , retightening 5 times and retightening after 10 cycles of dynamic loading was effective for maintaining rtv in internal type implants.conclusionretightening of screws is more effective in internal type implants than external type implants . 
 retightening of screws is recommended in the early stage of functional loading . 