multiple sclerosis ( ms ) is a chronic inflammatory - demyelinating disease of the central nervous system ( cns ) .
it is characterised by multifocal and disseminated in time damage to the cns with heterogeneous symptomatology and the clinical course .
depending on the studied region , the prevalence in the general population varies from 5 to 150 per 100,000 individuals .
the first symptoms usually appear between the ages of 20 and 40 [ 2 , 3 ] and the disease is the most frequently observed among caucasians , with a higher prevalence in females . currently , ms is a disease of complex pathogenesis with genetic , immunopathological and environmental factors [ 4 , 5 ] .
the protective effect of sunlight seems to be related to the protective action of 25(oh)d3 , which plays an important role in immunological processes and in the regulation of calcium  phosphate metabolism .
recent studies have demonstrated that 25(oh)d3 participates in cell proliferation and differentiation , and also in immunological phenomena .
the greatest 25(oh)d3 deficiency was noted in the spring and in the winter and also in the elderly .
females aged 1545  years are at a greater risk of 25(oh)d3 deficiency , especially in combination with seasonal variations .
the peak of incidence is noted between the third and the fourth decades of life [ 8 , 9 ] .
to provide complex medical care , it is important to determine the indices of calcium  phosphate metabolism in patients with ms .
the study evaluated the concentration of 25(oh)d3 and the indices of calcium  phosphate metabolism depending on the sex , number of relapses , degree of motor disability and the applied treatment at different stages of relapsing  remitting ms ( rrms ) .
the study evaluated the concentration of 25(oh)d3 and the indices of calcium  phosphate metabolism depending on the sex , number of relapses , degree of motor disability and the applied treatment at different stages of relapsing  remitting ms ( rrms ) .
patients with diagnosed rrms were enrolled in the study , according to the mcdonald criteria of 2005 .
the patients were selected based on the medical documentation of the out - patient neurological clinic and the medical history of patients hospitalised between 2009 and 2011 in the clinic of neurology in zabrze , poland .
the enrolment criteria were as follows : diagnosis of rrms according to the mcdonald criteria ; the current degree of disability according to the expanded disability status scale ( edss ) from 0.0 to 3.0 points ; inhabitation of the silesian agglomeration ( at a latitude of 4950 , n ) ; performance of the professional work under similar environmental conditions ; absence of any other chronic disease except ms , patients informed written consent for the study .
recruited patients had not followed any diet which might have influenced the calcium  phosphate metabolism ( e.g. diabetic diet , weight - reduction diet , high - protein diet , etc . ) .
patients had not consumed dietary supplements with vitamin d before or at the time of the study , and within 6  months prior to sample collection had not resided in other climatic zones .
the exclusion criteria were as follows : cns demyelinating syndrome of different aetiology than ms ; forms of ms other than rrms ; duration of disease longer than 6  years and the degree of motor disability > 3.0 points according to the edss ; relapse at the time of examination ; treatment with immunomodulatory agents or glucocorticoids ( gcs ) for the mean duration of 6 or 12  weeks depending on the qualification to a study subgroup ; diagnosed kidney disease , gastrointestinal tract and liver diseases , endocrine disorders , menopausal women .
the results were compared to values obtained in 20 age- and sex - matched controls who did not differ in terms of place of residence or ethnicity .
the study was approved by the bioethics committee of the medical university of silesia in katowice , poland .
all enrolled patients were interviewed with particular attention paid to the course of rrms : onset of symptoms , year of diagnosis , additional tests , number of relapses ( exacerbations of the disease manifested with new neurological symptoms ) , hospitalisations , previous treatment , family and occupational history and comorbidities .
general and neurological examination was conducted and the degree of motor disability of patients was evaluated using the edss . in every patient ,
5  ml fasting blood sample was obtained and daily ( 24  h ) urine sample was collected .
laboratory tests evaluated the indices of calcium  phosphate metabolism , the results of which were compared to the applicable standards for specific test methods .
serum concentration of 25(oh)d3 ( normal range 11.142.9  ng / ml ; 30.4118.5  nmol / l ) and parathormone concentration ( normal range 1565  pg / ml ) were assessed using the cobas e 601 analyser ( roche ) .
serum phosphorus concentration ( normal range 0.811.45  mmol / l ) , serum calcium concentration ( normal range 2.12.55  mmol / l ) , alkaline phosphatase concentration ( normal range 3090 u / l ) , bone alkaline phosphatase concentration ( normal range 2048 u / l ) , and concentration of daily urine calcium excretion ( normal range 2.56.5  mmol / l/24  h ) were assessed using the cobas c 501 analyser ( roche ) .
mmol / l ) was measured directly using ion - selective electrodes ( rapid lab 865 , siemens ) .
samples for laboratory analyses were collected in april and the sun exposure was similar for all the examined subjects .
the results were stored in the database prepared specifically for this purpose in microsoft excel .
the result of the statistical analysis was considered statistically significant if the level of significance was p    0.05 . in data description ,
the standard statistical parameters were provided , i.e. the number n , arithmetic mean x , the sd , median and percentages ( % ) . normal distribution of data was assessed by the shapiro 
the results were compared with the control group ( table  1).table  1the characteristics of the study groupsgroup 1group 2group 3control groupnumber of subjects ( n)15151520stage of diseaseimmediately after the diagnosis of rrmsin the early stage of rrms up to 6  months after the occurrence of first symptomsin the advanced stage of rrms 56  years after the occurrence of first symptomshealthy individuals , not diagnosed with any cns disease or any other systemic or metabolic disorderstreatment usednot treated with immuno - modulatory agents and gcssubjects not used immunomodulatory agents and were not treated with gcs 6  weeks prior to study enrolmentsubjects not used immuno - modulatory agents and were not treated with gcs 12  weeks prior to study enrolmentnot treated with immuno - modulatory agents or gcssex ( f / m)*9 ( 60  % ) /6 ( 40  % ) 10 ( 66.7  % ) /5 ( 33.3  % ) 12 ( 80  % ) /3 ( 20  % ) 14 ( 70  % ) /6 ( 30  % ) age ( years)**35.3    12.133.5    8.936.2    6.135.2    8.2edss ( points)**1.2    0.651.2    0.441.8    0.7number of relapses before the enrolment to the study**1.3    1.12.4    2.13.5    2.4 
 rrms relapsing  remitting multiple sclerosis , gcs glucocorticoids , cns central nervous system , f female , m male edss expended disability status scale *  data presented as n ( % ) * *  data presented as mean    sd the characteristics of the study groups 
 rrms relapsing  remitting multiple sclerosis , gcs glucocorticoids , cns central nervous system , f female , m male edss expended disability status scale *  data presented as n ( % ) * *  data presented as mean    sd in the studied groups , serum ionised calcium concentrations differed significantly ( p  =  0.002 ) .
the most significant differences regarding the concentrations of ionised calcium were observed between group 1 and group 2 ( p  =  0.004 ) , and between group 1 and the individuals in the control group who had higher concentrations compared to patients in group 1 ( p  =  0.003 ) ( tables  2 , 3).table  2laboratory results in the examined groupsparameterstatistical parametergroup 1 
 n  =  15group 2 
 n  =  15group 3 
 n  =  15control group 
 n  =  20kruskal  wallistestserum phosphorus ( mmol / l ) 
 x 
 1.071.131.011.06ns ( p  =  0.130)sd0.230.20.170.16me1.051.21.031.0710  % p0.870.930.880.990  % p1.191.311.181.23serum calcium ( mmol / l ) 
 x 
 2.382.372.372.32ns ( p  =  0.160)sd0.140.20.080.07me2.402.362.392.3110  % p2.202.252.252.2590  % p2.562.442.452.42serum alkaline phosphatase ( u / l ) 
 x 
 57.263.96256.7ns ( p  =  0.240)sd10.716.818.722.1me5559564910  % p45.443.444.838.990  % p72.88588.285.4serum bone alkaline phosphatase ( u / l ) 
 x 
 21.522.822.121.8ns ( p  =  0.900)sd8.17.77.29.6me22232120.510  % p1212.415.41190  % p26.632.231.232.2serum ionised calcium ( mmol / l ) 
 x 
 1.11.141.091.18 

p  =  0.002 
 sd0.210.060.050.17me1.071.141.081.1410  % p0.991.071.051.0690  % p1.141.21.141.28serum parathormone ( pg / ml ) 
 x 
 24.3361.0176.752.28 
 p  =  0.00001 
 sd11.2534.9741.9723.68me22.5960.164.3948.9310  % p7.3616.0637.1127.2490  % p36.86103.221158282.88serum 25(oh)d3 ( ng / ml ) 
 x 
 20.316.9113.8926.28 

p  =  0.007 
 sd10.388.446.488.24me17.7515.5913.428.0910  % p9.57.415.7616.6990  % p31.8129.2921.1138.33daily urine calcium excretion ( mmol / l/24  h ) 
 x 
 2.362.673.133.79ns ( p  =  0.310)sd0.981.92.532.68me2.161.861.673.0410  % p1.211.261.291.1790  % p3.575.137.477.42 
 x mean , sd standard deviation , me median , 10  % p , 90  % p percentiles , ns not significant , p level of significancetable  3the comparisons between pairs of groups for serum ionised calcium , parathormone and 25(oh)d3 concentration values ( mann  whitney u test)comparisonionised calciumparathormone25(oh)d3 
 group 1group 2 
 p  =  0.004 

 p 
<  0.001 
 ns ( p  =  0.300)group 1group 3ns ( p  =  0.250 ) 
 p  <  0.001 
 ns ( p  =  0.090)group 1control group 
 p  =  0.003 

 p  <  0.001 
 ns ( p  =  0.060)group 2group 3 
 p  =  0.020 
 ns ( p  =  0.590)ns ( p  =  0.490)group 2control groupns ( p  =  0.880)ns ( p  =  0.230 ) 
 p  =  0.002 
 group 3control group 
 p  =  0.020 

 p  = 
0.050 

 p  <  0.001 
 significant values ( p    0.05 ) are in bold laboratory results in the examined groups 
 x mean , sd standard deviation , me median , 10  % p , 90  % p percentiles , ns not significant , p level of significance the comparisons between pairs of groups for serum ionised calcium , parathormone and 25(oh)d3 concentration values ( mann  whitney u test ) significant values ( p    0.05 ) are in bold an association was found between the mean parathormone concentrations and the disease duration . in group 3 ,
the mean serum concentration of parathormone was the highest and was above the upper limit of the reference range .
newly diagnosed patients had the lowest serum concentration of parathormone compared to serum concentration of parathormone in patients at a more advanced stage of the disease as well as in the control group ( table  2 ) . a decrease in mean concentrations of 25(oh)d3 with the disease duration
the mean serum concentration of 25(oh)d3 in the studied groups of patients was lower compared to the control group ( p  =  0.007 ) . a significant difference in concentrations
was demonstrated between the control group and patients with the disease duration of up to 6  months ( p  =  0.002 ) or longer ( p  <  0.001 ) ( tables  2 , 3 ) .
next , the indices of calcium  phosphate metabolism were assessed depending on the sex . in group 3 ( patients with longer disease duration ) , lower concentrations of 25(oh)d3 were found in females compared to males . in the group of patients with a shorter disease duration (
groups 1 and 2 ) , the mean serum concentration of 25(oh)d3 was higher in females compared to males with a similar degree of motor disability .
the mean serum concentrations of parathormone in male patients at the advanced stage of the disease ( group 3 ) were higher compared to females . both in females in group 2 and in all patients in group 3 ,
regardless of the sex , the mean concentrations of parathormone exceeded the upper limit of the reference range ( table  4).table  4parameters of calcium  phosphate metabolism in the examined groups , depending on sex ( mean    sd)parametersexgroup 1  ( n  =  15)group 2  ( n  =  15)group 3  ( n  =  15)control group ( n  =  20)serum phosphorus ( mmol / l )  m1.10    0.351.07    0.291.03    0.170.94    0.14f1.06 
  0.121.17    0.151.00    0.171.11    0.14serum calcium ( mmol / l )  m2.41    0.102.35    0.052.39   
0.042.35    0.10f2.37    0.162.37    0.252.37    0.082.31    0.06serum alkaline phosphatase ( u / l)m63.2    12.766.0    21.566.0    28.076.2    24.3f53.2    7.962.8    15.261.0    17.348.4    15.3serum bone alkaline phosphatase ( u / l)m20.7    8.021.6    6.926.3    3.228.5    12.1f22.1    8.723.4   
8.421.0    7.618.9    7.0serum ionised calcium ( mmol / l)m1.03    0.041.13    0.041.06    0.011.15    0.12f1.14    0.271.15    0.081.09    0.061.19    0.19serum parathormone
( pg / ml)m22.72    9.3447.03    40.5683.18    48.2251.55   
19.61f25.40    12.8068.00    31.7675.07    42.4852.59    25.92serum 25(oh)d3 ( ng / ml)m18.22    7.0414.61    10.3517.45    6.1124.48    9.38f21.70    12.3418.05    7.6713.00    6.5027.05    7.95daily urine calcium excretion ( mmol / l/24  h )  m2.24    0.994.16    2.632.66    1.344.76    1.88f2.44   
1.021.92    0.843.25    2.783.37    2.92 
 m male , f female parameters of calcium  phosphate metabolism in the examined groups , depending on sex ( mean    sd ) the increase in serum parathormone concentration was observed with the increase in relapses ( r  =  0.51 , p  <  0.001 ) in the conducted correlation analysis between the number of relapses , the degree of disability and the indices of calcium  phosphate metabolism in all patients .
the present study showed that in patients with rrms , serum concentrations of 25(oh)d3 and ionised calcium were significantly lower compared to healthy individuals and decreased with the duration of the disease , with the increase in relapses and in females compared to males .
our results concerning the concentration of 25(oh)d3 in rrms patients are compatible with those of some [ 10 , 11 ] , but not all [ 1215 ] prior publications ( table  5 ) .
moen et al . , kragt et al . , soilu - hanninen et al . , and barnes et al .  
presented different results obtained in norway , the netherlands , finland , great britain and northern ireland .
the researchers showed comparable mean 25(oh)d3 concentrations in patients with ms and healthy persons.table  5summary table with data from the past publications and the present studykubicka - baczyk et al.past publicationssmolders et al.van der mei et al.hussein et al.moen et al.pierrot-deseilligny et al.kragt et al.barnes et al.el-ghoneimy et al.place of the studypolandregion silesiamaastricht , netherlandstasmania , australiacair , egyptoslo , norwayparis , franceamsterdam , netherlandsulster , northern irelandcair , egyptlatitude4950n5051n4143s30n5955n4852n5222n5435n30nnumber of subjects ( n)6543893599761012920age ( years)*35.0    9.034.1    10.143.5    9.341    1045.0    11.946.79    11.328.7    6.0edss ( points)1.41    0.692.68    1.743.5    2.24.09    1.91.4    1.12.343.4    2.3serum 25(oh)d3 
 ( nmol / l)*54.16    26.8179.47    32.2nd51.4    20.333.65    22.768.4    24.549    22nd78.65    29.669.19    40.031.8    14.31ndserum parathormone ( pmol / l)*82.64    44.983.55    1.895.35    1.8561.18    26.0165.24    37.3ndserum ionised calcium ( mmol / l)*1.11    0.111.27    0.032.36    0.090.66    0.29nd * data presented as mean    sd ,   
results in ms patients were significantly lower than in controls ,    results in ms patients
results in ms patients were comparable to controls ; nd not defined ( results in ms patients were not compared to controls ) ,    not studied summary table with data from the past publications and the present study * data presented as mean    sd ,    results in ms patients were significantly lower than in controls ,  
results in ms patients were comparable to controls ; nd not defined ( results in ms patients were not compared to controls ) ,    not studied in the present study , the relationship between 25(oh)d3 concentration and the degree of motor disability according to the edss was also analysed and no correlation was found .
contrary results were obtained by van der mei et al . , hussein et al .   and smolders et al . .
patients with a higher serum concentration of 25(oh)d3 had a better motor ability . in the present study
, an increase was observed in the mean concentration of parathormone with disease duration and the simultaneous decrease in the mean concentration of 25(oh)d3 .
however , the study results of the soilu - hanninen et al . were different .
they showed lower serum concentrations of parathormone and ionised calcium in patients with rrms compared to healthy persons . in the present study ,
the concentrations of mineral metabolism indices ( i.e. phosphorus , alkaline phosphatase and bone alkaline phosphatase ) were within the reference range , which is consistent with the results obtained in scandinavia and france [ 15 , 18 ] . in the present study in patients with rrms diagnosed within 6  months and about 6  years prior to the study ,
higher concentrations of parathormone and ionised calcium were found and lower 25(oh)d3 concentrations were observed compared to patients with the shortest disease duration .
a group of patients with rrms in whom an increased parathormone concentration was observed was in remission .
this is contrary to the results of soilu - hanninen et al .   and pierrot - deseilligny et al .   who examined the mineral metabolism in patients during an ms relapse
due to the fact that ms occurs more frequently in females , in the present study the attention was paid to the concentration of the examined indices , depending on the sex of patients . according to kuchuk
, the largest deficiency of 25(oh)d3 in females appears during the greatest fertility between the ages of 15 and 45  years .
the peak of prevalence for ms occurs at the same time . analysing the data from the present study , a significantly lower serum concentration of 25(oh)d3
moreover , in the group of patients with a longer disease duration a significantly lower 25(oh)d3 concentration in females was detected compared to male patients with the similar degree of motor disability .
evaluated 25(oh)d3 concentration depending on the sex of individuals inhabiting regions of different sun exposure .
the authors are of the opinion that the obtained results may be determined by cultural and religious factors of the communities inhabiting arabic countries .
in patients with rrms , serum concentrations of 25(oh)d3 and ionised calcium were significantly lower compared to healthy individuals and decreased with the duration of the disease , with the increase in relapses and in females compared to males .
however , the concentration of parathormone increased significantly as rrsm progressed . due to the geographical location , poland is one of the countries with a high risk of ms incidence .
however , studies on calcium  phosphate metabolism have not been conducted in poland as yet .
the role of the deficiency of 25(oh)d3 in patients with ms immediately after the diagnosis and/or the increasing 25(oh)d3 deficiency in the course of disease progression requires further research as well as the determination of indices of mineral metabolism depending on the treatment used and in the relapse or remission .
the results of the present study and the available literature show that it is appropriate to determine the indices of calcium  phosphate metabolism , including 25(oh)d3 in patients immediately after diagnosing ms and in the course of disease progression . depending on the results ,
the limitation of the present study is a small number of patients with rrms and healthy controls .
the limitation of the present study is a small number of patients with rrms and healthy controls .
 introduction and objectivesthe purpose of this study was to evaluate the concentration of 25-hydroxycholecalciferol and parameters of calcium  phosphate metabolism at different periods of relapsing  remitting multiple sclerosis ( rrms).materials and methodsforty - five patients , residents of poland ( 4950 , n ) , were enrolled in the study , i.e. 15 immediately after the diagnosis of rrms , 15 at the early stage and 15 at the advanced stage of rrms . 
 the results were compared to values obtained in 20 age- and sex - matched controls.resultslower serum concentrations of 25-hydroxycholecalciferol and ionised calcium were found in patients compared to the control group . in patients with the disease duration of 56  years , concentrations of 25-hydroxycholecalciferol and 
 ionised calcium were lower than in patients in the earlier period of rrms . 
 the inverse and clearer direction of changes was found in parathormone serum concentration in patients compared to the controls . in patients with a longer disease duration , 
 a significantly lower 25-hydroxycholecalciferol concentration was found in female patients compared to male patients . in patients , more frequent 25-hydroxycholecalciferol and unsaturated fatty acids supplementation 
 was observed compared to the controls.conclusionsin rrms patients , calcium  phosphate metabolism is disturbed which increases during disease progression . 