encouragingly , the boolean model was able to make highly accurate predictions on spatial and temporal gene expression patterns .
indeed , only several predictions among the 2,772 time - space - gene combinations were at odds with experimental data .
this may not seem surprising because the grn is based on interpretations of huge masses of expression data , perturbation data and cis - regulatory data .
proceeded to perform more stringent tests of the model by asking how it would respond to four perturbations : extinction of the expression of the delta gene , global expression of the pmar1 gene , extinction of hox11/13b expression , and most challengingly , the transplantation of four cleavage skeletogenic micromeres into the animal pole of an otherwise normal embryo that possessed its own set of four micromeres at the vegetal pole .
( the intra - embryo transposition of blastomeres harkens back to the classical era of experimental embryology . )
the authors emphasized that except for the hox11/13b test , the perturbation results they sought to reproduce were not used in building the grn .
( transplanted micromeres could of course not have been part of the grn , which is for a normal embryo . )
the results of these perturbation tests were in nearly perfect agreement with the experimental data , which led the authors to two conclusions : the grn contained sufficient information to provide a system - level causal explanation for sea urchin development , and the boolean computational model was a useful tool for in silico testing of grn and making predictions upon perturbations .
the test of blastomere transplantation demonstrated the critical role of intercellular signaling between the different spatial domains in development .
the cells in these domains obviously need to work cooperatively to ensure precise and robust developmental progression and thus information on the regulatory state of each cell must be able to diffuse spatially .
we enjoy so many examples of such events in development , e.g. , the wnt pathway in drosophila development to mention just one of many examples , but we have no case in which a paracrine signaling pathway amidst a developmentally determinative cluster of cells can be put into the context of a grn as detailed as the one peter et al . have defined . from the information theory perspective , intercellular
thus , the developmental process is also a diffusion process of the genomic regulatory information .
related domains of this emerging field include  molecular information theory  and information networks in the data mining field from both of which the modeling of intercellular signaling may benefit .
went so far as to suggest that the gene regulatory models could sufficiently explain all the gene expression patterns in sea urchin development , without considering non - coding rnas .
for example , a recent study identified long noncoding rnas ( lncrna ) in zebrafish embryogenesis and revealed that lncrnas were specifically enriched in early - stage embryos .
ironically , davidson and his longtime partner roy britten were the first to postulate such a role .
is there any information loss from the continuous data to boolean data , from gene regulatory logic to boolean logic ? what would be a good cutoff for converting the expression level to  on  or  off  ?
if a grn is not available in a given case , can a boolean model be directly inferred and tested using raw experimental data ?
if so , what kinds of experimental data are most suitable and in what way can the causal structure be best captured in the boolean model ?
theoretical frameworks of causal inference from observational data have been studied in machine learning for decades .
possibly the established causal inference algorithms can expand this newest chapter in grn - ology from the pioneering davidson lab into more general settings .
can this work provide insights into other animal development , e.g. , mouse , human or into human embryonic stem ( es ) cell differentiation ?
is it possible to apply the general computational framework to test grn models derived from biological processes in addition to development , such as immunology and postnatal neurogenesis in the subventricular zone of the brain to mention only two of many frontiers before us that beckon for the grn approach ? with the advances of next generation sequencing technology and its cost now rocketing downward , a large amount of data will soon be in hand for various biological processes across diverse phyla .
much of this momentum now comes from the sea urchin embryo and from the scientific mind of eric davidson .
 eric davidson at caltech has spent several decades investigating the molecular basis of animal development using the sea urchin embryo as an experimental system1,2 although his scholarship extends to all of embryology as embodied in several editions of his landmark book.3 in recent years his laboratory has become a leading force in constructing gene regulatory networks ( grns ) operating in sea urchin development.4 this axis of his work has its roots in this laboratory s cdna cloning of an actin mrna from the sea urchin embryo ( for the timeline , see ref . 
 1)one of the first eukaryotic mrnas to be cloned as it turned out . 
 from that point of departure , the davidson lab has drilled down into other genes and gene families and the factors that regulate their coordinated regulation , leading them into the grn era ( a field they helped to define ) and the development of the computational tools needed to consolidate and advance the grn field . 