Ipa re ninu ere The Adventures of Priscilla, Queen of the Desert (1994) mu ki o gba ami-eye BAFTA Award o si tun mu ki won yan mo ara awon ti o to si ami eye Golden Globe Award.. 
O ti gba ọpọlọpọ ami-eye, awọn bi Primetime Emmy Award, won si ti yan mo awon ti o to si ami-eye Academy Awards léméjì, BAFTA Award, screen Actors Guild Awards, ati ami eye Golden Globe Awards lémẹ́ta.Malkovich bẹrẹ iṣẹ osere gege bi nigba ti o darapọ mo egbe steppenWolf Theatre company ni Chicago ni ọdun 1976.. 