Question: How to identify new species using molecular phylogenetics?
Hi! My current task is to identify fungal strain which genome has been sequenced. To do so I've built several phylogenetic trees (single-gene: 18s SSU, 28s LSU, ITS and multi-gene: ITS + 28s LSU + RPB2). Data set for such analysis contained sequences from two orders (BLAST search showed affiliation with this two orders). Topology of trees tells that target strain may be a new species or may be insertae sedis.
What kind of analysis should be performed in order to proof that target strain is a new species or may be insertae sedis or none of that?
One of the multi-gene trees for one of the orders is shown on picture.