Cultured Mouse bone marrow cells.
ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
Our Abpromise guarantee covers the use of ab105157 in the following tested applications.
Mouse (strain C57BL6) peripheral granulocytes stained with ab105157.
Publishing research using ab105157? Please let us know so that we can cite the reference in this datasheet.
Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached).
You contacted Abcam back in November 2011 about a problem with ab105157 in flow cytometry. I was wondering if the problems had been resolved or if you are still having problems with it.
Please let me know if there is anything I can help you with.
Thank you for your inquiry. The concentration of specific antibody in this product cannot be accurately calculated because of the interference in the reading from the PE protein itself. We can estimate that this product is 0.1mg/ml, but again, this is an estimate. I hope this information helps. Please contact us with any other questions.
Hi I wonder about the quantity of antibody in your ´anti-neutrophil antibody 7/4` product. (ab 105157). Is it comparable to the FITC-conjugated antibody?
Hello, Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached). Could it be anything wrong with this specific bottle?
Thank you for contacting us and for including your attached flow data. I agree the vial of ab105157 you received is not working as expected. If you could forward me the purchase order number or Abcam order reference number associated with the purchase I would be happy to send you a replacement.
Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached). Could it be anything wrong with this specific bottle?
Thank you for contacting Abcam. I am sorry that the current lot of ab105157 is not working in flow cytometry. The data you provide clearly shows that there is a difference in the results between the old and the new antibody. Would you be able to send me the lot number for the current antibody you have and the lot number for the old antibody, if you still have that information? I would like to see if there is any information about these specific lots that could account for such a difference. I look forward to your reply and helping you resolve this issue.