Hi Patrick,  Perhaps this post by my colleague Rick Mack might help: How to enable Single Signon (SSO)in vWorkspace for Vista/Windows 7 clients with Kerberos against Server 2008 R2  Cheers, Dave 
We are moving from our Proof-of-Concept into a vWorkspace Pilot and I need to have the AppPortal Auto-Launch a Desktop Application (VM) after the user logs into the repurposed desktop computer.  Everything have been setup and works fine if the user authenticates at each entry point (PC, AppPortal, VM) but fails during the autologin due to the requirement of NLA.  I have set and checked all required settings and configurations within Group Policy, however the login's continue to fail.  I discovered that our GP's are still based off of Server 2003 but the needed Server 2008 R2 / Windows 7 GPO templates were imported.  Does anyone know what might be the problem?  Thank!  Patrick
Network Level Authentication - AppPortal Auto-Launch Application