Hi MIke,  I've raised a case for you and will send you the log information through the case.  Thanks.
Hiya All,  Environment:   vWorkspace Farm consists of one Windows Server 2008 R2 Server setup with RDSH and Quest vWorkspace 8 - One Windows Server 2003 R2 (32-bit) Server with Terminal Services. Workstations are a mix of 32 and 64 bit machines running Windowws 7 and XP  Situation:   Users are using AppPortal to run published apps.  As long as the app is published on the 2008R2 Server all is good and doesn't really fail.  Issue comes with the apps that are being published from the 32bit 2003R2 Server.  It seems that quite often I have to reboot the server to get those published apps to work again.  End user will go to run the app and comes up with the error  " Error launching application [appname] - There are no available terminal servers to connect to at this time.  Please report this message to your sys admin. (-2147019860)"   I cannot reproduce the problem manually.  If I simply reboot, or even just restart the Quest services on the server users can connect up again.  The system can go days without an issue, or this issue can occur multiple times in a single day. The clients are auto-configured using an XML file that actually does not enable NAT Support for Firewall Transversal.  So, Knowledge Article 107875 does not seem to apply.
No available terminal servers error