in accordance with the method of the present invention , an aqueous resazurin ( diazoresorcinol ) solution is reduced partially or fully by the metabolizing sperm in a semen sample , which typically is one full ejaculate . the collective rate of metabolism of the sperm in the semen sample is a measure of the potential fertility of the semen sample . solutions of resazurin change color in the presence of metabolizing sperm . these color changes are sufficiently pronounced in aqueous solutions of appropriate concentration so as to be readily apparent when compared to standard color samples , such as , for example , standardized color chips on a chart . therefore , such color changes are a directed measurement of the fertility of the semen sample . in accordance with a preferred embodiment of the method , the resazurin solution is prepared by dissolving resazurin crystals in a normal saline solution ( 0 . 9 percent ) at a convenient concentration , e . g ., 50 mg of resazurin per 100 milliliter of saline solution . this forms a reagent dye solution which is initially deep blue or almost black in color . upon mixing this reagent dye with a semen sample in a ratio of one drop ( 0 . 05 ml ) of dye solution per approximately one milliliter of semen , the semen sample becomes blue or purple in color . more dilute resazurin solutions produce correspondingly less dramatic color changes which are more difficult to evaluate and more concentrated solutions produce problems in maintaining the desired ratio of dye to semen volume . over a selected period of time , for example one hour at 48 ° c ., the sample changes color to a redder shade if the sperm therein are sufficiently numerous and mobile to reduce the dye in the sample to a significant extent . the degree of color change in a sample is indicative of the extent to which the resazurin is reduced as a result of the collective metabolizing rate of sperm in the sample and will range anywhere from only a slight shift from its initial shade of blue or purple to a pink shade . the novel kit used to practice the method of the instant invention relies on the ability of sperm to reduce the selected reagent dye , such as resazurin , and , in the process , effect a change in the color or shade of the reagent dye . in that this change in color or shade is a function of both sperm count and sperm motility , both of which are related to fertility , the test method provides an assessment of the potential fertility of the semen sample containing the sperm . it should be kept in mind that the correlation of sperm characteristics and fertility is not a perfect correlation , because on occasion , pregnancies can result even with low sperm counts and poor sperm motility . moreover , some men with normal sperm specimens are unable to achieve a pregnancy with their partners . although there is no known test of sperm potential fertility that is one hundred percent accurate , it is believed that measuring the collective metabolism rate of all of the sperm in an ejaculate may be the most accurate criteria of the potential fertility of the human male donor . except when there is complete absence of motile sperm in the semen sample , which is always associated with infertility , the values for sperm analysis based on sperm count and sperm motility against which the home test kit in accordance with the instant invention is compared for accuracy lack definite threshold figures separating fertility from infertility . a sperm count equal to or greater than twenty million sperm per milliliter is generally considered to be in the fertile range , whereas a lower count is considered subfertile . however , there is some disagreement on this level and some laboratories and clinicians use a level of even forty million sperm per milliliter in making a fertile determination . it has been found , however , that twenty percent of the human males with sperm counts below twenty million sperm per milliliters will father a child and as many as fifteen percent of human males with &# 34 ; normal &# 34 ; sperm counts may have some degree of sperm dysfunction that interferes with fertility . similarly , sperm specimens with below normal motility are not invariably associated with infertility . there is also disagreement over what constitutes the lower limit of sperm motility that can be considered normal , with estimates ranging between forty to sixty percent . consequently , the current standard for assessing human male fertility is not one hundred percent accurate and in all likelihood is less than ninety percent accurate in categorizing human males as fertile or infertile . in addition , both false positive and false negative results are not uncommon in laboratory sperm analysis . although the home test method and kit according to the instant invention may reflect some of the inaccuracies inherent in conventional sperm analysis , it could be more accurate than the sperm analysis generally performed by laboratories in that the kit measures collective sperm metabolism . accordingly , a discrepancy between the results of a home test performed in accordance with the instant invention and the results of a conventional laboratory sperm analysis does not necessarily mean that the home test is erroneous . in the absence of a more precise test for human male fertility , the standard laboratory sperm analysis is the only standard comparison now available for the home kit in accordance with the instant invention . a primary advantage of the home test kit is that it will encourage men having a low assessment of potential fertility to seek medical attention while those who have a high assessment of potential fertility will have an indication that lack of fertility may reside elsewhere . generally , in a situation where the test kit indicates a high assessment of fertility potential , a couple would be well advised to attempt to conceive for a period of four months before seeking medical advice on their fertility . there are a number of benefits to utilizing the test kit in accordance with the instant invention , one of which is that the individual will be able to assess the potential fertility of his sperm specimen in the privacy of his home . another benefit is that men undergoing treatment for infertility can utilize the home test kit to monitor changes in sperm metabolism to determine whether or not the treatment is effective . another advantage is that the evaluation can always be made with a fresh semen sample and the sperm is not exposed to cold temperatures which can be encountered on the way to the laboratory . another advantage is that the home test kit will lower the cost of human male fertility evaluation in that it is less expensive for an individual to utilize the home test kit than to have his sperm analyzed at a laboratory . the test kit also can be used by a doctor who does not have ready access to or who does not choose to utilize the facilities of a laboratory equipped to conduct a conventional sperm analysis . the test kit also can be used to evaluate the fertility of stored , e . g ., liquid nitrogen frozen sperm . the frozen sperm sample would be thawed to room temperature prior to mixing with the appropriate volume of dye . considering now more specifically the test kit in accordance with the instant invention and the use thereof to practice the method of the instant invention , it is seen that the kit comprises a plurality of relatively simple components which can be packaged in a box . the components include a collection tube 10 , a funnel 11 , a cap 12 , a support 13 for the test tube 10 , an insulating jacket 14 for a drinking glass 16 ( not supplied in kit ), a pipet 17 containing the dye to be reduced by the sperm in the semen sample , a disposable thermometer 18 and a color chart 19 with which the sample in the test tube is compared in the presence of light from an ordinary incandescent lamp 21 . the person who wishes to test his fertility utilizes the aforementioned components of the kit in accordance with the following procedure . after abstaining from an ejaculation for a period of at least seventy - two hours , an ejaculate is collected in the tube 10 using the funnel 11 screwed onto the threaded mouth thereof to guide the ejaculate into the tube . in order to increase the convenience of collection , the axis of the funnel 11 is at an angle of forty - five degrees with respect to the axis of the tube 10 . the tube 10 with the funnel 11 therein is then supported vertically by inserting the tube 10 into a hole 23 in the support 13 which support may be , for example , a cardboard box and maintained in that position for twenty minutes at room temperature , to permit the semen in the funnel 11 to drain into the tube 10 . during that twenty minute period , the sperm specimen will become more liquid . it is not necessary for all of the semen to drip into the tube 10 , and a small amount can stay behind in the funnel 11 . after a substantial portion of the semen has drained into the tube 10 , the funnel 11 is removed and discarded . the tube 10 has a number of gradations or lines inscribed on the side thereof , e . g ., representing milliliters or fractions of a fluid ounce , indicated by the numerals &# 34 ; 1 &# 34 ;, &# 34 ; 2 &# 34 ;, &# 34 ; 3 &# 34 ;, &# 34 ; 4 &# 34 ;, &# 34 ; 5 &# 34 ;. the level of the semen sample in the tube 10 is compared to the line on the test tube nearest the upper surface of the semen specimen . this indicates the volume of the specimen in the tube or other unit volume of the sample . the numeral on the side of the tube 10 indicative of the volume of the semen sample determines the number of drops of dye solution to be dispensed from the pipet 17 into the semen sample . the pipet 17 containing the dye solution is preferably packaged in a conventional plastic wrapper and has the dye solution sealed therein . to dispense the dye solution , the small diameter end 24 of the pipet 17 is snipped off with a pair of scissors and a couple of drops of the dye solution are expelled from the pipet 17 and discarded , e . g ., into a toilet bowl , in order to discharge any air from the tubular portion 26 of the pipet . the pipet 17 is then aligned with the top of the tube 10 and slowly squeezed to dispense the appropriate number of drops therefrom corresponding to the numeral aligned with the gradation on the test to which the top of the semen sample most closely aligns . for example , if the level of semen rises to the numeral &# 34 ; 2 &# 34 ;, then two drops of a 50 mg / 100 ml dye solution are dispensed from the pipet 17 ; if the level in the test tube 10 rises to the numeral &# 34 ; 3 &# 34 ;, then three drops of dye are dispensed from the pipet , and so forth . if a more dilute dye solution is employed , an appropriately larger number of drops of the dye solution are dispensed . the cap 12 is then placed on the test tube 10 to close the test tube . the next procedure to be followed in conducting the test is to insert a standard 8 - ounce drinking glass 16 into the insulating jacket 14 . the insulating jacket 14 furnished with the kit is of a size which surrounds the cylindrical surface of the glass 16 as well as underlying the bottom of the glass . the test bath is prepared by filling the drinking glass approximately two - thirds full with hot , but not boiling , tap water . the plastic strip thermometer 18 is then inserted into the water with all of the numbers submerged . the plastic strip thermometer is preferably a conventional disposable thermometer and indicates temperature by a green circle which appears just above the numeral corresponding to the temperature of the bath . in accordance with the instant invention wherein the dye is resazurin , the initial temperature of the bath should be about forty - six degrees to forty - eight degrees celsius . if the green circle is above fifty degrees centigrade , an appropriate amount of cooler water should be added after discarding some of the water in the glass . when the correct temperature is achieved , i . e ., 46 °- 48 ° c . in accordance with the preferable dye used in the instant invention , the strip thermometer 18 is withdrawn from the bath and discarded . as is seen in fig4 the tube 10 with the cap 12 in place is then agitated by shaking the test tube three or four times in order to thoroughly mix the semen and the dye solution . if the dye solution used is a 50 mg / 100 ml resazurin solution as previously described , the specimen will be deep blue or purple after mixing . as is seen in fig5 the tube 10 is then placed in the bath of tap water with the cap end of the test tube up . the tube 10 will partially float with the upper portion resting against the side of the glass 14 . the person conducting the test then writes down the time the tube 10 was inserted in the bath and leaves the test tube in the bath for a period of one hour . during this time , the tube is in the bath and the glass 14 is maintained on a flat surface away from open windows or air conditioners which might excessively cool the bath , viz ., below about 34 ° c . in a very cool room , it may be necessary to replace the water with warmer water during the hour holding period to ensure that the test mixture does not become too cool . after one hour has passed , the test tube 10 is removed from the bath and shaken two or three times . the test result is determined by comparing the color of the specimen in the test tube 10 with the various colors on the color chart 19 while the color chart and test tube are bathed in daylight or light from a conventional incandescent lamp 21 . fluorescent light ordinarily can not be used because it often distorts color reflected from the chart and specimen . the color chart 19 has a plurality of color chips 31a - 31k positioned adjacent one edge thereof on a white background 32 . the color chart 19 is preferably divided into a low fertility region 33 , indicated by a minus sign or a designation &# 34 ; negative &# 34 ;, and a high fertility region 34 , indicated by a plus sign or a designation &# 34 ; positive &# 34 ;. since the test tube 10 is transparent , the color of the semen - dye mixture can be viewed against the white background 32 of the color chart 19 and compared to the various chips 31a - 31k , which range in color from plum purple to bright pink . in making an assessment as to the potential fertility of the sperm , the chip among color chips 31a - 31k which most closely matches the color of the semen - dye mixture is indicative of the sperm metabolism of the sample . if the chip among color chips 31a - 31k which is matched is in the positive region 34 , then the subject knows that at the time the semen sample was taken his potential fertility was high , whereas if the chip is in the low region 33 , the subject knows that at the time his semen sample was taken his potential fertility was low . no change in color is strongly indicative of infertility . referring now to fig7 wherein alternative methods of examining semen samples for fertility are diagrammatically illustrated , it is seen that the semen sample , instead of being visually examined as shown in fig6 is examined by a device such as a spectrophotometer , colorometer or fluorometer 40 . by utilizing a suitable test tube 10 , which is in the form of a cuvette , readily insertable into the device , an automatic determination can be made as to whether or not the semen sample is capable of fertilizing an egg . assuming that the device 40 is a spectrophotometer , the semen sample which is either diluted or undiluted in distilled water is placed in the spectrophotometric cuvette 10 . exemplary of a spectrophotometer which can accomplish the aims of the instant invention is the perkin - elmer lamda iii uv / vis spectrophotometer which is calibrated automatically and reads specimens in the cuvettes individually . negative semen samples indicating infertility give a maximum absorbency at a wavelength of 600 nanometers . in a negative reading , the initial and final color of the semen sample is dark blue . a positive semen sample has an initial color of dark blue but changes to reddish pink and provides only baseline absorbency . the device 40 could be either a spectrophotometer or an equivalent colorometer since these instruments are interchangeable operating on the same physical principle so as to produce the same results . if the device 40 is a fluorometer , such as the digital fluorometers turner models 112 and 450 , quantitative analysis of fluorescence is accomplished during the chemical reaction in which resazurin which is dark blue in color is reduced by cell metabolism to resorufin which is pink in color . referring now to fig8 there is shown an additional embodiment of the invention wherein the semen sample is analyzed by a fluorescent microscope 48 rather than being analyzed visually as is shown in fig6 . with this approach , a portion of the semen sample is placed on a microscope slide 46 , covered with a cover slip 47 and viewed through a fluorescent microscope 48 which may be , for example , a standard zeiss fluorescent microscope . if the semen sample is positive for fertility , it will appear red when a filter for a fluorescent stain , such as rhodamine , is used . only a dark background is visible when the filter for fluorescein is used . fluorescein is another fluorescent stain that fluoresces at a different wavelength than rhodamine . if the population and motility of the sperm in the semen sample is not high enough to reduce the dye , then there will be no fluorescence when the sample being reviewed is viewed through a filter for rhodamine or a filter for fluorescein . from the foregoing description , one skilled in the art can easily ascertain the essential characteristics of this invention , and without departing from the spirit and scope thereof , can make various changes and modifications of the invention to adapt it to various usages and conditions .