EDTA'nın keşfini kimlerden elde etmiştir?
etilendiamin ve klorasetik asit çözeltilerinden
EDTA (Ethylenediaminetetraacetic acid): = EDTA Nedir?:= EDTA, Etilendiamin tetraasetik asit'in kısaltmasidir. EDTA polyamino karboksilik asid bileşiğidir. Genel formülü, [CH2N(CH2COOH)2]2 şeklindedir. Yapısal formülü için, http://upload.wikimedia.org/wikipedia/commons/thumb/8/85/Ethylenediaminetetraacetic.png/200px- Ethylenediaminetetraacetic.png = EDTA'nın Kimyasal Özellikleri:= CAS No: 60-00-4 Molekül Formülü: C10H16N2O8 Molekül Ağırlığı: 292.24 g.mol−1 Yoğunluğu: 0,86 g/cm3 Erime noktası: 240 °C Asidite, pK1=0.0 (CO2H) (µ=1.0) pK2=1.5 (CO2H) (µ=0.1) pK3=2.00 (CO2H) (µ=0.1) pK4=2.69 (CO2H) (µ=0.1) pK5=6.13 (NH+) (µ=0.1) pK6=10.37 (NH+) (µ=0.1) = EDTA'nın Keşfi:= EDTA ilk olarak Ferdinand Munz tarafından tanımlanmıştır. Munz, EDTA'nın keşfini etilendiamin ve klorasetik asit çözeltilerinden elde etmiştir. H2NCH2CH2NH2 \+ 4 CH2O + 4 NaCN + 4 H2O → (NaO2CCH2)2NCH2CH2N(CH2CO2Na)2 \+ 4 NH3 (NaO2CCH2)2NCH2CH2N(CH2CO2Na)2 \+ 4 HCl → (HO2CCH2)2NCH2CH2N(CH2CO2H)2 \+ 4 NaCl Poliamino karboksilik asid grubundan olan EDTA’nın kimyasal yapısına bakıldığında 2 amino 4 karboksil ligandı vardır. EDTA metal iyonlarına karşı yüksek afinite gösterir. [Fe(H2O)6]3+ \+ H4EDTA \rightleftharpoons [Fe(EDTA)]−\+ 6 H2O + 4 H+ (Keq = 1025.1) = EDTA’nın Laboratuvarda Kullanımı:= EDTA laboratuvarda metal iyonlarını tutmak için kullanılır. Biyokimya ve moleküler biyoloji iyon tüketici olarak enzimlere karşı kullanılır. Analitik kimya da kompleksometrik titrasyon, Su sertliği maskeleyici ajanlar analizelerinde kullanılır. = Kaynakça:= 1 ^ Harris, D.C. "Quantitative Chemical Analysis", 7th ed., W. H. Freeman and Compagny, New York, 2007 2 ^ F. Munz "Polyamino carboxylic acids to I. G. Farbenindustrie, DE 718 981, 1935, US 2 130 505, 1938. 3 ^ Synthesis of EDTA 4 ^ a b c J. Roger Hart "Ethylenediaminetetraacetic Acid and Related Chelating Agents" in Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH, Weinheim, 2005. 5 ^ Kirchner, S. Barium (Ethylenediaminetetracetato) Cobalt(III) 4-Hydrate" Inorganic Syntheses, 1957, Volume 5, pages 186-188. 6 ^ Edta - Motm 7 ^ Holleman, A. F., Wiberg, E. (2001). Inorganic Chemistry. San Diego: Academic Press. ISBN 0-12-352651-5. 8 ^ Furia T (1964). "EDTA in Foods — A technical review". Food Technology 18 (12): 1874–1882. 9 ^ a b c Lanigan RS and Yamarik TA (2002). "Final report on the safety assessment of EDTA, calcium disodium EDTA, diammonium EDTA, dipotassium EDTA, disodium EDTA, TEA-EDTA, tetrasodium EDTA, tripotassium EDTA, trisodium EDTA, HEDTA, and trisodium HEDTA". Int J Toxicol. 21 Suppl 2: 95–142. http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db,=PubMed&list,_uids=12396676&dopt,=AbstractPlus . Retrieved 2008-01-28. 10 ^ US Food and Drug Administration: Center for Food Safety and Applied Nutrition Questions and Answers on the Occurrence of Benzene in Soft Drinks and Other Beverages 11 ^ Ruth DeBusk et al. (2002). "Ethylenediaminetetraacetic acid (EDTA)". http://www.umm.edu/altmed/articles/ethylenediaminetetraacetic-acid-000302.htm . Retrieved 2007-07-25. 12 ^ "Home > Medical Reference > Complementary Medicine > EDTA overview". University of Maryland Medical Center. http://www.umm.edu/altmed/articles/ethylenediaminetetraacetic-acid-000302.htm . Retrieved 16 December 2009. 13 ^ Loren, Karl (1996). "I/V Chelation Using EDTA Life Flow One The Solution For Heart Disease". The Thinking Person's Guide to Perfect Health. Vibrant Life. http://www.oralchelation.net/data/EDTA/data14e.htm . Retrieved 16 December 2009. 14 ^ See "les conservateurs en opthalmologie" Doctors Patrice Vo Tan & Yves lachkar, Librarie Médicale Théa. 15 ^ Green, Saul, Wallace Sampson (December 14, 2002). "EDTA Chelation Therapy for Atherosclerosis And Degenerative Diseases: Implausibility and Paradoxical Oxidant Effects". Quackwatch. http://www.quackwatch.org/01QuackeryRelatedTopics/chelationimp.html . Retrieved 16 December 2009. 16 ^ http://www.clinicaltrials.gov/ct/show/NCT00044213?order=2 17 ^ Margolock, David (July 26, 1995). "F.B.I. Disputes Simpson Defense on Tainted Blood". The New York Times: pp. A12. http://query.nytimes.com/gst/fullpage.html?sec=health&res,=990CE4DD1F3DF935A15754C0A963958260 . Retrieved 16 December 2009. 18 ^ Zhiwen Yuan, Jeanne M. VanBriesen "The Formation of Intermediates in EDTA and NTA Biodegradation" Environmental Engineering Science 2006, volume 23, pp. 533–544. doi:10.1089/ees.2006.23.533 19 ^ a b Robin L. Sheppard, and Jack Henion (1997). "Determining EDTA in Blood" ([dead link] – Scholar search). Analytical Chemistry 69: 477A–480A. http://pubs.acs.org/hotartcl/ac/97/aug/det.html20+Ocak+1998+tarihinde+Wayback+Machine+sitesinde+arşivlendi.. Retrieved 2007-07-25. 20 ^ S. Loyaux-Lawniczak, J. Douch, and P. Behra (1999). "Optimisation of the analytical detection of EDTA by HPLC in natural waters". Fresenius' J. Anal. Chem. 364 (8): 727–731. doi:10.1007/s002160051422. https://web.archive.org/web/20071012154504/http://cat.inist.fr/?aModele=afficheN&cpsidt,=1898737.