Who played the lead role in the play which most clearly reflects Shakespeare's relationship with his sovereign?
Lord Chamberlain's Men: The Lord Chamberlain's Men was a company of actors, or a "playing company" as it would have been known, for which Shakespeare wrote for most of his career. Lord Chamberlain's Men:  Richard Burbage played most of the lead roles, including Hamlet, Othello, King Lear, and Macbeth while Shakespeare himself performed some secondary roles. Macbeth:  Of all the plays that Shakespeare wrote during the reign of James I, who was patron of Shakespeare's acting company, "Macbeth" most clearly reflects the playwright's relationship with his sovereign.
Richard Burbage