
Phosphatome

The phosphatome of an organism is the set of phosphatase genes in its genome. Phosphatases are enzymes that catalyze the removal of phosphate from biomolecules. Over half of all cellular proteins are modified by phosphorylation which typically controls their functions. Protein phosphorylation is controlled by the opposing actions of protein phosphatases and protein kinases.
Most phosphorylation sites are not linked to a specific phosphatase, so the phosphatome approach allows a global analysis of dephosphorylation, screening to find the phosphatase responsible for a given reaction, and comparative studies between different phosphatases, similar to how protein kinase research has been impacted by the kinome approach.
The Protein Phosphatome.
Protein phosphatases remove phosphates from proteins, usually on Serine, Threonine, and Tyrosine residues, reversing the action of protein kinases. The PTP family of protein phosphatases is tyrosine-specific, and several other families (PPPL, PPM, HAD) appear to be serine/threonine specific, while other families are unknown or have a variety of substrates (DSPs dephosphorylate any amino acid, while some protein phosphatases also have non-protein substrates). In the human genome, 20 different folds of protein are known to be phosphatases, of which 10 include protein phosphatases.
Protein phosphatomes have been cataloged for human and 8 other key eukaryotes, for Plasmodium and Trypanosomes
and phosphatomes have been used for functional analysis, by experimentally investing all known protein phosphatases, in the yeast Fusarium, in Plasmodium and in human cancer
Large scale databases exist for human and animal phosphatomes Phosphatome.net, parasitic protozoans ProtozPhosDB and for the substrates of human phosphatases DEPOD.
Non-Protein Phosphatases.
Non-protein phosphorylation has three general forms
The human non-protein phosphatome has been cataloged, but most phosphatome analyses are restricted to protein and lipid phosphatases that have regulatory functions.
Pseudophosphatases.
The phosphatome includes proteins that are structurally closely related to phosphatases but lack catalytic activity. These retain biological function, and may regulate pathways that involve active phosphatases, or bind to phosphorylated substrates without cleaving them. Examples include STYX, where the phosphatase domain has become a phospho-tyrosine binding domain, and GAK, whose inactive phosphatase domain instead binds phospholipds.