Barr body

A Barr body (named after discoverer Murray Barr) is an inactive X chromosome in a cell with more than one X chromosome, rendered inactive in a process called lyonization, in species with XY sex-determination (including humans). The Lyon hypothesis states that in cells with multiple X chromosomes, all but one are inactivated during mammalian embryogenesis. This happens early in embryonic development at random in mammals, except in marsupials and in some extra-embryonic tissues of some placental mammals, in which the X chromosome from the sperm is always deactivated.
[ The inactive X chromosome can be seen in the interphase nucleus as a darkly staining small mass in contact with the nucleus membrane known as Barr body or X - chromatin. ]
In humans with more than one X chromosome, the number of Barr bodies visible at interphase is always one fewer than the total number of X chromosomes. For example, people with Klinefelter syndrome (47,XXY karyotype) have a single Barr body, and people with a 47, XXX karyotype have two Barr bodies. Barr bodies can be seen in the nucleus of neutrophils, at the rim of the nucleus in female somatic cells between divisions.
Mechanism.
Someone with two X chromosomes (such as most human females) has only one Barr body per somatic cell, while someone with one X chromosome (such as most human males) has none.
Mammalian X-chromosome inactivation is initiated from the X inactivation centre or "Xic", usually found near the centromere. The center contains twelve genes, seven of which code for proteins, five for untranslated RNAs, of which only two are known to play an active role in the X inactivation process, "Xist" and "Tsix". The centre also appears to be important in chromosome counting: ensuring that random inactivation only takes place when two or more X-chromosomes are present. The provision of an extra artificial "Xic" in early embryogenesis can induce inactivation of the single X found in male cells.
The roles of "Xist" and "Tsix" appear to be antagonistic. The loss of "Tsix" expression on the future inactive X chromosome results in an increase in levels of "Xist" around the "Xic". Meanwhile, on the future active X "Tsix" levels are maintained; thus the levels of "Xist" remain low. This shift allows "Xist" to begin coating the future inactive chromosome, spreading out from the "Xic". In non-random inactivation this choice appears to be fixed and current evidence suggests that the maternally inherited gene may be imprinted. Variations in Xi frequency have been reported with age, pregnancy, the use of oral contraceptives, fluctuations in menstrual cycle and neoplasia.
It is thought that this constitutes the mechanism of choice, and allows downstream processes to establish the compact state of the Barr body. These changes include histone modifications, such as histone H3 methylation (i.e. H3K27me3 by PRC2 which is recruited by Xist) and histone H2A ubiquitination, as well as direct modification of the DNA itself, via the methylation of CpG sites. These changes help inactivate gene expression on the inactive X-chromosome and to bring about its compaction to form the Barr body.
Reactivation of a Barr body is also possible, and has been seen in breast cancer patients. One study showed that the frequency of Barr bodies in breast carcinoma were significantly lower than in healthy controls, indicating reactivation of these once inactivated X chromosomes.
References.
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