Hippocampus anatomy

Hippocampus anatomy describes the physical aspects and properties of the hippocampus, a neural structure in the medial temporal lobe of the brain. It has a distinctive, curved shape that has been likened to the sea-horse monster of Greek mythology and the ram's horns of Amun in Egyptian mythology. This general layout holds across the full range of mammalian species, from hedgehog to human, although the details vary. For example, in the rat, the two hippocampi look similar to a pair of bananas, joined at the stems. In primate brains, including humans, the portion of the hippocampus near the base of the temporal lobe is much broader than the part at the top. Due to the three-dimensional curvature of this structure, two-dimensional sections such as shown are commonly seen. Neuroimaging pictures can show a number of different shapes, depending on the angle and location of the cut.
Topologically, the surface of a cerebral hemisphere can be regarded as a sphere with an indentation where it attaches to the midbrain. The structures that line the edge of the hole collectively make up the so-called limbic system (Latin "limbus" =
"border"), with the hippocampus lining the posterior edge of this hole. These limbic structures include the hippocampus, cingulate cortex, olfactory cortex, and amygdala. Paul MacLean once suggested, as part of his triune brain theory, that the limbic structures constitute the neural basis of emotion. While most neuroscientists no longer believe in the concept of a unified "limbic system", these regions are highly interconnected and do interact with one another.
Basic hippocampal circuit.
Starting at the dentate gyrus and working inward along the S-curve of the hippocampus means traversing a
series of narrow zones. The first of these, the dentate gyrus (DG), is actually a separate
structure, a tightly packed layer of small granule cells wrapped around the end of the hippocampus proper, forming a pointed wedge in some cross-sections, a semicircle in others. Next
come a series of "Cornu Ammonis" areas: first CA4 (which underlies the dentate gyrus), then CA3, then
a very small zone called CA2, then CA1. The CA areas are all filled with densely packed Pyramidal cells similar to those found in the neocortex. After CA1 comes an area called the subiculum. After this comes a pair of ill-defined areas called the presubiculum and parasubiculum, then a
transition to the cortex proper (mostly the entorhinal area of the cortex). Most anatomists
use the term "hippocampus proper" to refer to the four CA fields, and hippocampal formation
to refer to the hippocampus proper plus dentate gyrus and subiculum.
The major signaling pathways flow through the hippocampus and combine to form a loop. Most external input comes from the adjoining entorhinal cortex, via the axons of the so-called perforant path. These axons arise from layer 2 of the entorhinal cortex (EC), and terminate in the dentate gyrus and CA3. There is also a distinct pathway from layer 3 of the EC directly to CA1, often referred to as the temporoammonic or TA-CA1 pathway. Granule cells of the DG send their axons (called "mossy fibers") to CA3. Pyramidal cells of CA3 send their axons to CA1. Pyramidal cells of CA1 send their axons to the subiculum and deep layers of the EC. Subicular neurons send their axons mainly to the EC. The perforant path-to-dentate gyrus-to-CA3-to-CA1 was called the trisynaptic circuit by Per Andersen, who noted that thin slices could be cut out of the hippocampus perpendicular to its long axis, in a way that preserves all of these connections. This observation was the basis of his "lamellar hypothesis", which proposed that the hippocampus can be thought of as a series of parallel strips, operating in a functionally independent way. The lamellar concept is still sometimes considered to be a useful organizing principle, but more recent data, showing extensive longitudinal connections within the hippocampal system, have required it to be substantially modified.
Perforant path input from EC layer II enters the dentate gyrus and is relayed to region CA3 (and to mossy cells, located in the hilus of the dentate gyrus, which then send information to distant portions of the dentate gyrus where the cycle is repeated). Region CA3 combines this input with signals from EC layer II and sends extensive connections within the region and also sends connections to strata radiatum and oriens of ipsilateral and contralateral CA1 regions through a set of fibers called the Schaffer collaterals, and commissural
pathway, respectively. Region CA1 receives input from the CA3 subfield, EC layer III and the nucleus reuniens of the thalamus (which project only to the terminal apical dendritic tufts in the stratum lacunosum-moleculare). In turn, CA1 projects to the subiculum as well as sending information along the aforementioned output paths of the hippocampus. The subiculum is the final stage in the pathway, combining information from the CA1 projection and EC layer III to also send information along the output pathways of the hippocampus.
The hippocampus also receives a number of subcortical inputs. In "Macaca fascicularis", these inputs include the amygdala (specifically the anterior amygdaloid area, the basolateral nucleus, and the periamygdaloid cortex), the medial septum and the diagonal band of Broca, the claustrum, the substantia innominata and the basal nucleus of Meynert, the thalamus (including the anterior nuclear complex, the laterodorsal nucleus, the paraventricular and parataenial nuclei, the nucleus reuniens, and the nucleus centralis medialis), the lateral preoptic and lateral hypothalamic areas, the supramammillary and retromammillary regions, the ventral tegmental area, the tegmental reticular fields, the raphe nuclei (the nucleus centralis superior and the dorsal raphe nucleus), the nucleus reticularis tegementi pontis, the periaqueductal gray, the dorsal tegmental nucleus, and the locus coeruleus.
The hippocampus also receives direct monosynaptic projections from the cerebellar fastigial nucleus.
Major fiber systems in the rat.
Angular bundle.
These fibers start from the ventral part of entorhinal cortex (EC) and contain commissural (EC◀▶Hippocampus) and Perforant path (excitatory EC▶CA1, and inhibitory EC◀▶CA2) fibers. They travel along the septotemporal axis of the hippocampus. Perforant path fibers, as the name suggests, perforate subiculum before going to the hippocampus (CA fields) and dentate gyrus.
Fimbria-fornix pathway.
Fimbria-fornix fibers are the hippocampal and subicular gateway "to" and "from" subcortical brain regions. Different parts of this system are given different names:
At the circuit level, the "alveus" contains axonal fibers from the DG and from Pyramidal neurons of CA3, CA2, CA1 and subiculum (CA1 ▶ subiculum and CA1 ▶ entorhinal projections) that collect in the temporal hippocampus to form the fimbria/fornix, one of the major outputs of the hippocampus. In the rat, some medial and lateral entorhinal axons (entorhinal ▶ CA1 projection) pass through alveus towards the CA1 stratum lacunosum moleculare without making a significant number of en passant boutons on other CA1 layers (Temporoammonic alvear pathway). Contralateral entorhinal ▶ CA1 projections almost exclusively pass through alveus. The more septal the more ipsilateral entorhinal-CA1 projections that take alvear pathway (instead of perforant path). Although subiculum sends axonal projections to alveus, subiculum ▶ CA1 projection passes through strata oriens and moleculare of subiculum and CA1. Cholinergic and GABAergic projections from MS-DBB to CA1 also pass through Fimbria. Fimbria stimulation leads to cholinergic excitation of CA1 O-LMR cells.
It is also known that extracellular stimulation of "fimbria" stimulates CA3 Pyramidal cells antidromically and orthodromically, but it has no impact on dentate granule cells. Each CA1 Pyramidal cell also sends an axonal branch to fimbria.
Hippocampal commissures.
Hilar mossy cells and CA3 Pyramidal cells are the main origins of hippocampal commissural fibers. They pass through hippocampal commissures to reach contralateral regions of hippocampus. Hippocampal commissures have dorsal and ventral segments. Dorsal commissural fibers consists mainly of entorhinal and presubicular fibers to or from the hippocampus and dentate gyrus. As a rule of thumb, one could say that each cytoarchitectonic field that contributes to the commissural projection also has a parallel associational fiber that terminates in the ipsilateral hippocampus. The inner molecular layer of dentate gyrus (dendrites of both granule cells and GABAergic interneurons) receives a projection that has both associational and commissural fibers mainly from hilar mossy cells and to some extent from CA3c Pyramidal cells. Because this projection fibers originate from both ipsilateral and contralateral sides of hippocampus they are called associational/commissural projections. In fact, each mossy cell innervates both the ipsilateral and contralateral dentate gyrus. The well known trisynaptic circuit of the hippocampus spans mainly horizontally along the hippocampus. However, associational/commissural fibers, like CA2 Pyramidal cell associational projections, span mainly longitudinally (dorsoventrally) along the hippocampus.
Commissural fibers that originate from CA3 Pyramidal cells go to CA3, CA2 and CA1 regions. Like mossy cells, a single CA3 Pyramidal cell contributes to both commissural and associational fibers, and they terminate on both principal cells and interneurons. A weak commissural projection connects both CA1 regions together. Subiculum has no commissural inputs or outputs. In comparison with rodents, hippocampal commissural connections are much less abundant in the monkey and humans. Although excitatory cells are the main contributors to commissural pathways, a GABAergic component has been reported among their terminals which were traced back to hilus as origin. Stimulation of commissural fibers stimulates DG hilar perforant path-associated (HIPP) and CA3 trilaminar cells antidromically.
Hippocampal cells and layers.
Hippocampus proper.
The hippocampus proper is composed of a number of subfields. Though terminology varies among authors, the terms most frequently used are dentate gyrus and the cornu ammonis (literally "Amun's horns", abbreviated CA). The dentate gyrus contains the fascia dentata and the hilus, while the CA is differentiated into fields CA1, CA2, CA3, and CA4.
However, the region known as CA4 is in fact the "deep, polymorphic layer of the dentate gyrus" (as clarified by Theodor Blackstad (1956) and by David Amaral (1978)).
Cut in cross section, the hippocampus is a C-shaped structure that resembles a ram's horns. The name "cornu ammonis" refers to the Egyptian deity Amun, who has the head of a ram. The horned appearance of the hippocampus is caused by cell density differentials and the existence of varying degrees of neuronal fibers.
In rodents, the hippocampus is positioned so that, roughly, one end is near the top of the head (the dorsal or septal end) and one end near the bottom of the head (the ventral or temporal end). As shown in the figure, the structure itself is curved and subfields or regions are defined along the curve, from CA4 through CA1 (only CA3 and CA1 are labeled). The CA regions are also structured depthwise in clearly defined strata (or layers):
Dentate gyrus.
The dentate gyrus is composed of a similar series of strata:
For an up-to-date "knowledge base" of hippocampal formation neuronal types, their biomarker profile, active and passive electrophysiological parameters, and connectivity one can refer to Hippocampome.org.