Countercurrent multiplication

A countercurrent mechanism system is a mechanism that expends energy to create a concentration gradient. 
It is found widely in nature and especially in mammalian organs. For example, it can refer to the process that is underlying the process of urine concentration, that is, the production of hyperosmotic urine by the mammalian kidney.
The ability to concentrate urine is also present in birds.
Countercurrent multiplication is frequently mistaken for countercurrent exchange, a similar but different mechanism where gradients are maintained, but not established.
Physiological principles.
The term derives from the form and function of the loop of Henle, which consists of two parallel limbs of renal tubules running in opposite directions, separated by the interstitial space of the renal medulla.
Details.
Countercurrent multiplication was originally studied as a mechanism whereby urine is concentrated in the nephron. Initially studied in the 1950s by Gottschalk and Mylle following Werner Kuhn's postulations, this mechanism gained popularity only after a series of complicated micropuncture experiments.
Pump: The Na+/K+/2Cl− transporter in the ascending limb of the loop of Henle helps to create a gradient by shifting Na+ into the medullary interstitium. The thick ascending limb of the loop of Henle is the only part of the nephron lacking in aquaporin—a common transporter protein for water channels. This makes the thick ascending limb impermeable to water. The action of the Na+/K+/2Cl− transporter therefore creates a hypoosmolar solution in the tubular fluid and a hyperosmolar fluid in the interstitium, since water cannot follow the solutes to produce osmotic equilibrium.
Equilibration: Since the descending limb of the loop of henle consists of very leaky epithelium, the fluid inside the descending limb becomes hyperosmolar.
Shift: The movement of fluid through the tubules causes the hyperosmotic fluid to move further down the loop. Repeating many cycles causes fluid to be near isosmolar at the top of Henle's loop and very concentrated at the bottom of the loop. Animals with a need for very concentrated urine (such as desert animals) have very long loops of Henle to create a very large osmotic gradient. Animals that have abundant water on the other hand (such as beavers) have very short loops.
The vasa recta have a similar loop shape so that the gradient does not dissipate into the plasma.
The mechanism of counter current multiplication works together with the vasa recta's counter current exchange to prevent the wash out of salts and maintain a high osmolarity at the inner medulla.