Aquatic toxicology

Aquatic toxicology is the study of the effects of manufactured chemicals and other anthropogenic and natural materials and activities on s at various levels of organization, from subcellular through individual organisms to communities and ecosystems. Aquatic toxicology is a multidisciplinary field which integrates toxicology, aquatic ecology and aquatic chemistry.
This field of study includes freshwater, marine water and sediment environments. Common tests include standardized acute and chronic toxicity tests lasting 24–96 hours (acute test) to 7 days or more (chronic tests). These tests measure endpoints such as survival, growth, reproduction, that are measured at each concentration in a gradient, along with a control test. Typically using selected organisms with ecologically relevant sensitivity to toxicants and a well-established literature background. These organisms can be easily acquired or cultured in lab and are easy to handle.
History.
While basic research in toxicology began in multiple countries in the 1800s, it was not until around the 1930s that the use of acute toxicity testing, especially on fish, was established. Over the next two decades, the effects of chemicals and wastes on non-human species became more of a public issue and the era of the "pickle-jar bioassays" began as efforts increased to standardize toxicity testing techniques. 
In the United States, the passage of the Federal Water Pollution Control Act of 1947 marked the first comprehensive legislation for the control of water pollution and was followed by the Federal Water Pollution Control Act in 1956. In 1962, public and governmental interests were renewed, in large part due to the publication of Rachel Carson’s "Silent Spring", and three years later the Water Quality Act of 1965 was passed, which directed states to develop water quality standards. Public awareness, as well as scientific and governmental concern, continued to grow throughout the 1970s and by the end of the decade research had expanded to include hazard evaluation and risk analysis. In the subsequent decades, aquatic toxicology has continued to expand and internationalize so that there is now a strong application of toxicity testing for environmental protection.
Aquatic toxicity tests.
Aquatic toxicology tests (assays): toxicity tests are used to provide qualitative and quantitative data on adverse (deleterious) effects on aquatic organisms from a toxicant. Toxicity tests can be used to assess the potential for damage to an aquatic environment and provide a database that can be used to assess the risk associated within a situation for a specific toxicant. Aquatic toxicology tests can be performed in the field or in the laboratory. Field experiments generally refer to multiple species exposure and laboratory experiments generally refer to single species exposure. A dose–response relationship is most commonly used with a sigmoidal curve to quantify the toxic effects at a selected end-point or criteria for effect (i.e. death or other adverse effect to the organism). Concentration is on the x-axis and percent inhibition or response is on the y-axis.
The criteria for effects, or endpoints tested for, can include lethal and sublethal effects (see Toxicological effects).
There are different types of toxicity tests that can be performed on various test species. Different species differ in their susceptibility to chemicals, most likely due to differences in accessibility, metabolic rate, excretion rate, genetic factors, dietary factors, age, sex, health and stress level of the organism. Common standard test species are the fathead minnow (Pimephales promelas), daphnids ("Daphnia magna", "D. pulex", "D. pulicaria", "Ceriodaphnia dubia"), midge (Chironomus tentans, C. riparius), rainbow trout (Oncorhynchus mykiss), sheepshead minnow (Cyprinodon variegatu), zebra fish ("Danio rerio"), mysids (Mysidopsis), oyster (Crassotreas), scud (Hyalalla Azteca), grass shrimp (Palaemonetes pugio) and mussels ("Mytilus galloprovincialis"). As defined by ASTM, these species are routinely selected on the basis of availability, commercial, recreational, and ecological importance, past successful use, and regulatory use.
A variety of acceptable standardized test methods have been published. Some of the more widely accepted agencies to publish methods are: the American Public Health Association, US Environmental Protection Agency (EPA), ASTM International, International Organization for Standardization, Environment and Climate Change Canada, and Organisation for Economic Co-operation and Development. Standardized tests offer the ability to compare results between laboratories.
There are many kinds of toxicity tests widely accepted in the scientific literature and regulatory agencies. The type of test used depends on many factors: Specific regulatory agency conducting the test, resources available, physical and chemical characteristics of the environment, type of toxicant, test species available, laboratory vs. field testing, end-point selection, and time and resources available to conduct the assays are some of the most common influencing factors on test design.
Exposure systems.
Exposure systems are four general techniques the controls and test organisms are exposed to the dealing with treated and diluted water or the test solutions.
Types of tests.
Acute tests are short-term exposure tests (hours or days) and generally use lethality as an endpoint. In acute exposures, organisms come into contact with higher doses of the toxicant in a single event or in multiple events over a short period of time and usually produce immediate effects, depending on absorption time of the toxicant. These tests are generally conducted on organisms during a specific time period of the organism’s life cycle, and are considered partial life cycle tests. Acute tests are not valid if mortality in the control sample is greater than 10%. Results are reported in EC50, or concentration that will affect fifty percent of the sample size.
Chronic tests are long-term tests (weeks, months years), relative to the test organism’s life span (>10% of life span), and generally use sub-lethal endpoints. In chronic exposures, organisms come into contact with low, continuous doses of a toxicant. Chronic exposures may induce effects to acute exposure, but can also result in effects that develop slowly. Chronic tests are generally considered full life cycle tests and cover an entire generation time or reproductive life cycle (“egg to egg”). Chronic tests are not considered valid if mortality in the control sample is greater than 20%. These results are generally reported in NOECs (No observed effects level) and LOECs (Lowest observed effects level).
Early life stage tests are considered as subchronic exposures that are less than a complete reproductive life cycle and include exposure during early, sensitive life stages of an organism. These exposures are also called critical life stage, embryo-larval, or egg-fry tests. Early life stage tests are not considered valid if mortality in the control sample is greater than 30%.
Short-term sublethal tests are used to evaluate the toxicity of effluents to aquatic organisms. These methods are developed by the EPA, and only focus on the most sensitive life stages. Endpoints for these test include changes in growth, reproduction and survival. NOECs, LOECs and EC50s are reported in these tests.
Bioaccumulation tests are toxicity tests that can be used for hydrophobic chemicals that may accumulated in the fatty tissue of aquatic organisms. Toxicants with low solubilities in water generally can be stored in the fatty tissue due to the high lipid content in this tissue. The storage of these toxicants within the organism may lead to cumulative toxicity. Bioaccumulation tests use bioconcentration factors (BCF) to predict concentrations of hydrophobic contaminants in organisms. The BCF is the ratio of the average concentration of test chemical accumulated in the tissue of the test organism (under steady state conditions) to the average measured concentration in the water.
Freshwater tests and saltwater tests have different standard methods, especially as set by the regulatory agencies. However, these tests generally include a control (negative and/or positive), a geometric dilution series or other appropriate logarithmic dilution series, test chambers and equal numbers of replicates, and a test organism. Exact exposure time and test duration will depend on type of test (acute vs. chronic) and organism type. Temperature, water quality parameters and light will depend on regulator requirements and organism type.
In the US, many wastewater dischargers (e.g., factories, power plants, refineries, mines, municipal sewage treatment plants) are required to conduct periodic whole effluent toxicity (WET) tests under the National Pollutant Discharge Elimination System (NPDES) permit program, pursuant to the Clean Water Act. For facilities discharging to freshwater, effluent is used to perform static-acute multi-concentration toxicity tests with "Ceriodaphnia dubia" (water flea) and "Pimephales promelas" (fathead minnow), among other species. The test organisms are exposed for 48 hours under static conditions with five concentrations of the effluent. The major deviation in the short-term chronic effluent toxicity tests and the acute effluent toxicity tests is that the short-term chronic test lasts for seven days and the acute test lasts for 48 hours. For discharges to marine and estuarine waters, the test species used are sheepshead minnow ("Cyprinodon variegatus"), inland silverside ("Menidia beryllina"), "Americamysis bahia", and purple sea urchin ("Strongylocentrotus purpuratus").
Sediment tests.
At some point most chemicals originating from both anthropogenic and natural sources accumulate in sediment. For this reason, sediment toxicity can play a major role in the adverse biological effects seen in aquatic organisms, especially those inhabiting benthic habitats. A recommended approach for sediment testing is to apply the Sediment Quality Triad (SQT) which involves simultaneously examining sediment chemistry, toxicity, and field alterations so that more complete information can be gathered. Collection, handling, and storage of sediment can have an effect on bioavailability and for this reason standard methods have been developed to suit this purpose.
Toxicological effects.
Toxicity can be broken down into two broad categories of direct and indirect toxicity. Direct toxicity results from a toxicant acting at the site of action in or on the organism. Indirect toxicity occurs with a change in the physical, chemical, or biological environment.
Lethality is most common effect used in toxicology and used as an endpoint for acute toxicity tests. While conducting chronic toxicity tests sublethal effects are endpoints that are looked at. These endpoints include behavioral, physiological, biochemical, histological changes.
There are a number of effects that occur when an organism is simultaneously exposed to two or more toxicants. These effects include additive effects, synergistic effects, potentiation effects, and antagonistic effects. An additive effect occurs when combined effect is equal to a combination or sum of the individual effects. A synergistic effect occurs when the combination of effects is much greater than the two individual effects added together. Potentiation is an effect that occurs when an individual chemical has no effect is added to a toxicant and the combination has a greater effect than just the toxicant alone. Finally, an antagonistic effect occurs when a combination of chemicals has less of an effect than the sum of their individual effects.
Terminology.
All terms were derived from Rand.
Significance in regulatory context.
In the United States, aquatic toxicology plays an important role in the NPDES wastewater permit program. While most wastewater dischargers typically conduct analytical chemistry testing for known pollutants, whole effluent toxicity tests have been standardized and are performed routinely as a tool for evaluating the potential harmful effects of other pollutants not specifically regulated in the discharge permits.
EPA's water quality program has published water quality criteria (for individual pollutants) and water quality standards (for water bodies) that were derived from aquatic toxicity tests.
Sediment quality guidelines.
While sediment quality guidelines are not meant for regulation, they provide a way to rank and compare sediment quality developed by National Oceanic and Atmospheric Administration(NOAA). These sediment quality guidelines are summarized in NOAA's Screening Quick Reference Tables (SQuiRT) for many different chemicals.