Ergtoxin

Ergtoxin is a family of toxins that can be isolated from the venom of several members of the Mexican scorpion genus of "Centruroides". These toxins target hERG (human Ether- à -go-go-Related Gene) potassium channels.
Sources.
The toxin is derived from venomous glands of the Mexican scorpions "Centruroides Noxius Hoffmann", "Centruroides elegans", "Centruroides sculpturatus", "Centruroides exilicauda", "Centruroides gracilis" and "Centruroides limpidus".
Chemistry.
Based on primary sequence alignment, there are 27 different Ergtoxins (ɣ-KTx's), all of which belong to the larger group of scorpion short chain toxins that affect K+ channels (KTx). Ergtoxins are polypeptides composed of 42 to 62 amino acid residues. The most studied is the 42-amino-acid-long Ergtoxin (ɣ-KTx1.1) with the following one-letter amino acid code: DRDSCVDKSRCAKYGYYQECQDCCKNAGHNGGTCMFFKCKCA.
This Ergtoxin sequence contains four disulfide bridges between Cys5-Cys23, Cys11-Cys34, Cys20-Cys24 and Cys23-Cys41 and has a molecular mass of 4730.8 ± 0.4 Da. Ergtoxin displays two clusters of amino acids, one hydrophobic and one hydrophilic. Its structure is stabilized by five hydrogen bonds, HN15-O34, HN33-O40, HN35-O38, HN38-O35, HN40-O33. 
All of the above data have led to the following prediction for its 3D Structure. The tertiary structure of Ergtoxin is comparable to that of another toxin called OSK1, in spite of sharing only 35% sequence identity 
Target.
Ergtoxin can decrease hERG K+ activity by 50% at a concentration of 10 nM. The binding of Ergtoxin to hERG K+ has been suggested to be dependent on hydrophobic interactions with the channel pore, specifically with a prominently exposed hydrophobic cluster of amino acids (Tyr 14, Phe 36 and Phe 37). It has also been shown that natural oxidation of Met 35 decreases the affinity of the molecule for the hERG K+ channels by three orders of magnitude, suggesting that Met35 is a critical residue for either polypeptide 3D folding or interaction of the toxin with the channel.
Mode of action.
Ergtoxin effects are a result of the toxin binding to voltage- gated K+ channels containing the Kv11.1 alpha subunit encoded by ether-a-go-go-genes (hERG1, hERG2 and hERG3) in the central nervous system of humans. The several subtypes of Ergtoxin (e.g. CeErg4 and CeErg5) block voltage-gated K+ hERG-related channels. Owing to small differences in sequence, the different toxins show selectivity towards specific hERG-channels.
Two concurrent modes of action for these toxins have been reported: 1.) blocking channel conductance by interacting with the outer vestibule of the channel or at the extracellular surface pore domains S5-S6, and 2.) interference with channel gating through interaction with the voltage-sensing domain S1-S4.
Toxicity.
Toxicity varies with Ergtoxin subtype. CnErgTx1 blocks ERG-channels located in endocrine, nerve and heart cells in several species, and is more toxic than the CsEKerg1 subtype.
Treatment and therapeutic use.
Ergtoxin may potentially have a role in treatment of patients with ovarian cancer by inhibiting the proliferation of cells and thus the progression of cancer. However, while hERG K+ channels are expressed by SK-OV-3 cancer cells, the specific mechanisms of channel function in proliferation and potential therapeutic uses for toxins targeting these channels are still not confirmed.