Phytochemical Analysis and In Vitro Effects of Allium fistulosum L. and Allium sativum L. Extracts on Human Normal and Tumor Cell Lines: A Comparative Study

Allium sativum L. (garlic bulbs) and Allium fistulosum L. (Welsh onion leaves) showed quantitative differences of identified compounds: allicin and alliin (380 µg/mL and 1410 µg/mL in garlic; 20 µg/mL and 145 µg/mL in Welsh onion), and the phenolic compounds (chlorogenic acid, p-coumaric acid, ferulic acid, gentisic acid, 4-hydroxybenzoic acid, kaempferol, isoquercitrin, quercitrin, quercetin, and rutin). The chemical composition determined the inhibitory activity of Allium extracts in a dose-dependent manner, on human normal cells (BJ-IC50 0.8841% garlic/0.2433% Welsh onion and HaCaT-IC50 1.086% garlic/0.6197% Welsh onion) and tumor cells (DLD-1-IC50 5.482%/2.124%; MDA-MB-231-IC50 6.375%/2.464%; MCF-7-IC50 6.131%/3.353%; and SK-MES-1-IC50 4.651%/5.819%). At high concentrations, the cytotoxic activity of each extract, on normal cells, was confirmed by: the 50% of the growth inhibition concentration (IC50) value, the cell death induced by necrosis, and biochemical determination of LDH, catalase, and Caspase-3. The four tumor cell lines treated with high concentrations (10%, 5%, 2.5%, and 1.25%) of garlic extract showed different sensibility, appreciated on the base of IC50 value for the most sensitive cell line (SK-MES-1), and the less sensitive (MDA-MB-231) cell line. The high concentrations of Welsh onion extract (5%, 2.5%, and 1.25%) induced pH changes in the culture medium and SK-MES-1 being the less sensitive cell line.


Introduction
Allium plants have been used in traditional medicine since ancient times. Currently, studies regarding the phytotherapeutical properties of Allium species are investigating the effects of Allium extracts and bioactive compounds [1,2]. Allium species are still studied Table 1. Elution time, analytical method characteristics, and determined concentrations of alliin, allicin, and phenolic acids and flavonoids in the Allium extract samples (results expressed as the mean value of 4 analyzed samples, n = 4). LOD-limit of detection, R 2 -coefficient of determination for the calibration curves (at six levels of concentrations). Indicated intervals represent the average ± standard deviations (n = 4). * The information regarding Allium sativum was previously published by Pârvu et al. [10].

Evaluation of the Acute Effect on Human Normal Cell Viability
To explore the Allium extracts toxicity on human normal fibroblasts (BJ) and human normal keratinocytes (HaCaT), the viability of these cells was assessed by an MTT assay at 24 h. As presented in Figure 1, the A. sativum extract inhibited the growth of BJ and HaCaT cells in a dose dependent manner, with the 50% of the growth inhibition concentration (IC 50 ) value of 0.8841% in the growth medium for BJ cells, equivalent to 8.841 mg/mL and 1.086% in the growth medium for HaCaT cells at 1.086% equivalent to 10.86 mg/mL. When calculating the equivalent of extract in mg/mL we used the concentrations of stock solutions for each extract, 1 g garlic bulbs in each mL and 1 g of Welsh onion leaves in each 1.2 mL.  A. fistulosum extract also presents inhibitory activity on both cell lines included in this study ( Figure 2). IC 50 value for BJ was 0.2433% in the growth medium; equivalent to 2.019 mg/mL, while for HaCaT cells IC 50 value was 0.6197% equivalent to 5.144 mg/mL.

Figure 1.
Twenty-four hour MTT Assay on BJ and HaCaT treated with Allium sativum. * p < 0.05, ** p < 0.01, and *** p < 0.001 when comparisons were made with the C group. Garlic hydroalcoholic extract induces cell death in a dose dependent manner in both cell lines. The control group is represented by the untreated cells, which were cultivated with standard cell culture medium.

Figure 2.
Twenty-four hour MTT Assay on BJ and HaCaT treated with Allium fistulosum. *** p < 0.001 when comparisons were made with the C group. Welsh onion hydroalcoholic extract induces cell death in a dose dependent manner in both cell lines. The control group is represented by untreated cell, which were cultivated with standard cell culture medium.
Both A. sativum and A. fistulosum extracts have dose-dependent antiproliferative potential. At concentrations higher than 1.25% extract in the cell culture medium, garlic extract had lower inhibitory potential on BJ and HaCaT proliferation. On the other hand, Welsh onion extract fully inhibited cell growth at 5% and 10% of extract in the cell culture medium, while below 1.25% of extract, the growth inhibitor was lowered in a dose dependent manner. The difference between extracts was visible at their IC50 values. Twenty-four hour MTT Assay on BJ and HaCaT treated with Allium fistulosum. *** p < 0.001 when comparisons were made with the C group. Welsh onion hydroalcoholic extract induces cell death in a dose dependent manner in both cell lines. The control group is represented by untreated cell, which were cultivated with standard cell culture medium.
Both A. sativum and A. fistulosum extracts have dose-dependent antiproliferative potential. At concentrations higher than 1.25% extract in the cell culture medium, garlic extract had lower inhibitory potential on BJ and HaCaT proliferation. On the other hand, Welsh onion extract fully inhibited cell growth at 5% and 10% of extract in the cell culture medium, while below 1.25% of extract, the growth inhibitor was lowered in a dose dependent manner. The difference between extracts was visible at their IC 50 values.
Normal human fibroblasts (BJ cells) and keratinocytes (HaCaT) were sensitive to garlic and Welsh onion extract with different half inhibitory doses (IC 50 ). Viability tests that were used in our study highlight the dose-dependent viability rate, with a very increased cell death for 100 mg/mL garlic extract and 86 mg/mL Welsh onion extract, which represent the 10% dose added to cells.
Growth inhibition was observed on the confocal microscopy analysis (Figures 3 and 4), morphological changes highlighting induced cell death in a dose dependent manner.

Morphological Evaluation
When cells are exposed to cytotoxic agents they tend to adapt if this is possible. At high concentrations of toxic compounds apoptosis is induced. During cell death the cytoskeleton collapses and the nuclear envelope disassembles and the DNA breaks into pieces, moreover the cell surface is modified. The intracellular mechanism of apoptosis is similar to almost all cells, caspases cleave key proteins in the cell, and some caspases cleave nuclear lamina or proteins that in normal situations keep DNAse in an inactive form, saving the DNA [52].
The most cytotoxic concentration seems to be the 10% garlic extract where the cytoskeleton and nucleus are almost gone, and the mitochondrial network is no more highlighted (D for BJ and H for HaCaT). The IC 50 concentration for both cell lines had no cytotoxic effect; the nucleus and cytoskeleton are very well highlighted, and the mitochondrial network seems to be very active (C for BJ and G for HaCaT). Cell structure and the morphological characteristics for cells that were treated with a lower extract dose than IC 50 (B for BJ and F for HaCaT) were comparable with the control group (A for BJ and E for HaCaT). These images confirmed the cytotoxic effect for both extracts at high concentration (10% extract) and less cytotoxic at IC 50 concentrations (0.8841% for BJ and 1.086% for HaCaT) and below IC 50 (0.5% for BJ and 0.1% for HaCaT), morphological modification with the disrupted cytoskeleton and mitochondrial network was indicating that cell death was induced by necrosis at 10% of extract. Morphological changes were less intense at IC 50 concentrations and below compared to 10% of extract. Normal human fibroblasts (BJ cells) and keratinocytes (HaCaT) were sensitive to garlic and Welsh onion extract with different half inhibitory doses (IC50). Viability tests that were used in our study highlight the dose-dependent viability rate, with a very increased cell death for 100 mg/mL garlic extract and 86 mg/mL Welsh onion extract, which represent the 10% dose added to cells.
Growth inhibition was observed on the confocal microscopy analysis (Figures 3 and 4), morphological changes highlighting induced cell death in a dose dependent manner.   Normal human fibroblasts (BJ cells) and keratinocytes (HaCaT) were sensitive to garlic and Welsh onion extract with different half inhibitory doses (IC50). Viability tests that were used in our study highlight the dose-dependent viability rate, with a very increased cell death for 100 mg/mL garlic extract and 86 mg/mL Welsh onion extract, which represent the 10% dose added to cells.
Growth inhibition was observed on the confocal microscopy analysis (Figures 3 and 4), morphological changes highlighting induced cell death in a dose dependent manner.   The cell integrity was investigated with a triple staining protocol, focusing on the mitochondrial networks, the cytoskeleton and the nucleus [12,51]. A. sativum is widely used for its antibacterial, antifungal, anti-inflammatory, and antitumoral properties, especially due to its organosulfur compounds [4,10,20,45]. Garlic extract showed a growth inhibitory effect after 24 h exposure to 100 mg/mL (equivalent to 10% extract), cell death aspects were visible, as presented in Figure 3D,H, the cytoskeleton collapsed, on BJ cells the nucleus was slightly visible, moreover the mitochondrial networks were not visible and the cell density was significantly reduced compared to the control group ( Figure 3A). At the IC 50 concentration (0.8841% for BJ and 1.086% for HaCaT) for both BJ and Ha-CaT cells the cytoskeleton networks showed minor damage and the cellular shape was modified ( Figure 3C,G). At concentrations below IC 50 (0.5% for BJ and 0.1% for HaCaT) the cytoskeleton was not damaged, while the mitochondrial networks were visible and the morphological characteristics ( Figure 3B,F) were comparable with the control group. HaCaT cells showed particular changes in mitochondrial networks at 5 mg/mL treatment with an intensified activity compared to the control group.
As presented in Figure 3, A. sativum extract inhibited cell growth in a dose dependent manner, this effect could be attributed to the complex mixture of bioactive compounds and due to the hydroalcoholic composition of the extract, thus inducing cellular stress.
A. fistulosum is a common Allium plant in Eastern Europe, rich in sulfur compounds and sterols, with antibacterial and antifungal properties [43]. As presented in Figure 4, at the highest dose of Welsh onion extract (83 mg/mL) induced cell death in both cell lines, the cytoskeleton was completely damaged, the mitochondrial networks and the nucleus were unclear and damaged ( Figure 4D,H). At the IC 50 dose, 2.019 mg/mL for BJ and 5.144 mg/mL for HaCaT, the cytoskeleton showed moderate damage, with focal adhesion points at HaCaT cells, which might be visible due to the usual distribution of these cells in small groups, with strong connections between cells ( Figure 4C,G). When adding 1 mg/mL of the extract on both cell lines ( Figure 4B,F), the cytoskeleton showed minor damage, the mitochondrial networks were visible, and the cell distribution and shape were comparable with the control group.
Welsh onion extract had a cytotoxic effect in a dose dependent manner, with small differences between the selected cell lines, BJ cells being more affected by the treatment compared with HaCaT; moreover, HaCaT cells had active mitochondrial networks highlighted at 1 mg/mL dose, while BJ cells had minor cytoskeleton damage and few mitochondrial networks with higher intensity ( Figure 4).
The most cytotoxic concentration seems to be the 10% Welsh onion extract where the cytoskeleton was disrupted and the nucleus was almost gone and the mitochondrial network was not as usual but was still visible for BJ cells and for HaCaT cells no mitochondria network could be highlighted (D for BJ and H for HaCaT). The IC 50 concentration for both cell lines had no cytotoxic effect; the nucleus and cytoskeleton were very well highlighted, and the mitochondrial network seemed to be very active (C for BJ and G for HaCaT). Cell structure and the morphological characteristics for cells that were treated with a lower extract dose than IC 50 (B for BJ and F for HaCaT) were comparable with the control group (A for BJ and E for HaCaT).
The highest concentrations that were used (10%, 5%, and 2.5% extract) were the most cytotoxic, cytoskeleton modifications were induced, and the cell shape was different compared to the control. Moreover, mitochondrial networks were less intense than control and the nucleus for treated cells with 100 mg/mL garlic extract and 86 mg/mL Welsh onion extract was no more visible or slightly visible. Confocal images are leading us to say that Allium extracts at a higher dose than IC 50 were inducing necrosis in normal fibroblasts and keratinocytes.
These images were indicating a cytotoxic effect for both extracts when concentration was high (10% extract) and a lower cytotoxic effect when the extract concentration was reduced, morphological modification with the disrupted cytoskeleton and mitochondrial network was indicating that cell death was induced by necrosis in the group treated with 10% extracts, while at lower concentrations of extracts cell populations were less affected and the structural damage was highlighted in a dose-dependent manner.

Biochemical Determination of LDH, CAT, and Caspase 3
Caspase 3 is a key molecule in the apoptotic pathway, being the node between the intrinsic and extrinsic apoptotic pathway. Increased activity of active Caspase 3 (Casp3) is correlated with apoptotic cells [53]. Allium species can induce apoptosis due to their organosulfur compounds, which increase Bax/Bcl-2 ration and enhance Casp3 activity [54].
A. sativum and A. fistulosum extracts slightly increased the activity of Casp3 in both cell lines, with a pronounced effect on keratinocytes when all three doses had increased the Casp3 activity compared to the control ( Figure 5). In order to confirm the apoptosis or the necrosis, the flow cytometry analysis should be taken into consideration, moreover, these results need to be correlated with lactate dehydrogenase (LDH) activity and catalase (CAT) activity for a complete understanding of the effect of both extracts on BJ and HaCaT cells.
Molecules 2021, 26, x FOR PEER REVIEW 8 of 21 Figure 5. For BJ cells, increased Casp3 was observed for 10% Allium sativum extract (100 mg/mL) and for 10% A. fistulosum extract (83 mg/mL), while for other concentrations there was no difference compared to the control group. As for HaCaT cells Casp3 was activated in a dose-dependent manner compared with the control group. Even if the statistical significance was very high, the numeric value of Casp3 activity was not so high and the results must be correlated with other parameters analyzed. ** p < 0.01, and *** p < 0.001 when comparisons were made with the C group.
At the highest dose, extracts acted like pro-oxidants and increased CAT activity, while around the IC50 and below the CAT activity was significantly lowered compared to the control group. These indicate that in a dose dependent manner, Allium extracts act differently depending on the cells that are used, moreover the shift between pro-oxidant and antioxidant effect can be made by changing the concentration of the extract that is used in the experiment. Increased CAT is correlated with high oxidative stress in cells. Lactate dehydrogenase (LDH) is a soluble enzyme located in the cytoplasm in almost all cells and it is released in the extracellular space when the plasma membrane is disrupted. Damaged membranes could be a marker for necrosis, moreover, during necrosis, the function and morphology of the organelles suffer changes, some of them have disrupted membranes or modified shape [56].
LDH release can be used as a marker for cytotoxicity; very active LDH in the supernatant is indicating that cell membranes have suffered modifications and cellular integrity was affected [41], both Allium extracts that were used in this study have a cytotoxic effect Figure 5. For BJ cells, increased Casp3 was observed for 10% Allium sativum extract (100 mg/mL) and for 10% A. fistulosum extract (83 mg/mL), while for other concentrations there was no difference compared to the control group. As for HaCaT cells Casp3 was activated in a dose-dependent manner compared with the control group. Even if the statistical significance was very high, the numeric value of Casp3 activity was not so high and the results must be correlated with other parameters analyzed. ** p < 0.01, and *** p < 0.001 when comparisons were made with the C group.
To discriminate the necrosis and apoptosis, LDH and CAT activity were measured by colorimetric assay. Catalase is an important enzyme involved in cell protection against oxidative damage. CAT catalyzes the decomposition of H 2 O 2 into H 2 O and O 2 [55]. Increased CAT activity is an indicator of increased oxidative stress, which may dysregulate normal physiological processes within the cells. An overexpressed CAT can reveal a high concentration of reactive oxygen species (ROS). Garlic extract has no effect on CAT activity in BJ cells at 10% of extract in the cell culture medium, while at IC 50 concentration the CAT activity was increased in BJ cells. On the other hand, on HaCaT cells, at 10% of garlic extract, CAT activity was increased, and decreased at lower doses ( Figure 6). In the case of Welsh onion extract, the effect was similar for both cell lines, at the highest concentration the CAT activity was increased, while at lower doses a decrease of CAT activity was observed.   At the highest dose, extracts acted like pro-oxidants and increased CAT activity, while around the IC 50 and below the CAT activity was significantly lowered compared to the control group. These indicate that in a dose dependent manner, Allium extracts act differently depending on the cells that are used, moreover the shift between pro-oxidant and antioxidant effect can be made by changing the concentration of the extract that is used in the experiment. Increased CAT is correlated with high oxidative stress in cells. Lactate dehydrogenase (LDH) is a soluble enzyme located in the cytoplasm in almost all cells and it is released in the extracellular space when the plasma membrane is disrupted. Damaged membranes could be a marker for necrosis, moreover, during necrosis, the function and morphology of the organelles suffer changes, some of them have disrupted membranes or modified shape [56].
LDH release can be used as a marker for cytotoxicity; very active LDH in the supernatant is indicating that cell membranes have suffered modifications and cellular integrity was affected [41], both Allium extracts that were used in this study have a cytotoxic effect depending on the dose that was added as treatment, with a more pronounced cytotoxic effect for the Welsh onion extract. Furthermore, HaCaT cells seem to be more sensitive to the A. fistulosum extract than BJ cells, as presented in Figure 7.
Cells that were treated with 100 mg/mL garlic and 86 mg/mL Welsh onion had released a large amount of LDH in the culture medium and had CAT with an increased activity indicating that oxidative stress was harming the cells and was disrupting cell membrane causing cell death. Caspase 3 was not so intense in its activity in none of the cells. LDH and CAT that were increased were indicating that cells might be in necrosis. To confirm or not confirm the necrosis, Annexin V/PI was performed, or samples were treated with the highest dose of 50 mg/mL garlic extract and 43 mg/mL Welsh onion extract. The results were encouraging us to confirm that Allium extracts had cytotoxic effects by inducing necrosis in treated cells in a dose-dependent manner and with a different intensity in BJ cells compared to HaCaT cells. Figure 6. Catalase was increased around the IC50 value of Allium sativum extract in BJ cells (1% extract equivalent to 10 mg/mL) and decreased in treatment with A. fistulosum; the numeric value of CAT activity is not so high, compared to HaCaT cells. Keratinocytes are more sensitive to A. sativum and A. fistulosum extract and the effect is different for each dose. * p < 0.05 and *** p < 0.001 when comparisons were made with the C group.  Doses between 0.062 and 1 mg/mL were used to investigate the ROS production after exposing the SCC-15 cell line to A. sativum extract, with results indicating a dose-dependent increasing ROS production, correlated with an increased LDH activity [41], similar to herein presented results on the LDH activity trend.

The Evaluation of Induced Cell Death
For completing the confocal images information and to characterize which type of cell death was induced in BJ and HaCaT cell lines, biochemical analysis, Caspase 3 quantification, and Annexin/PI Flow Cytometry Assay are coming to underline the effect of A. sativum and A. fistulosum extracts.
Annexin V/PI Assay is a golden standard assay to determine the percentage of viable, necrotic, or apoptotic cells. Untreated cells from HaCaT negative control group were 95% viable with 5% necrotic cells, while the positive control for HaCaT cells had 88.6% viable cells, 10.8% necrotic, 0.4% early apoptotic, and 0.2% late apoptotic cells. Necrosis was induced in a dose dependent manner in HaCaT cells by the A. sativum extract with the most intense necrosis for the 5% concentration (6.1% necrotic cells and 93.9% viable) and by the A. fistulosum extract with 7.9% necrotic cells at the sample treated with 5% extract. It is interesting that for HaCaT cells no apoptotic cell was detected, while for BJ cells only for the 0.5% garlic extract no apoptotic cell was detected. The negative control for BJ cells had 90.2% viable cells, 9.7% necrotic cells, and 0.1% late apoptotic cells, while the positive control had 84% viable and 16% necrotic cells. The most aggressive treatment was the 5% A. sativum extract with 19.3% necrotic cells, 0.1% early apoptotic, and 0.4% late apoptotic cells; moreover the A. fistulosum extract at 5% concentration in cell culture media induced necrosis in 16.8% of the cells and 0.5% late apoptosis. It is true that at the IC 50 concentration, both garlic (1% extract concentration) and Welsh onion (0.25% extract concentration) induced early apoptosis, 1.6% for BJ treated with garlic at IC 50 concentration and 2.6% early apoptosis and 1% late apoptosis for BJ treated with the Welsh onion extract at IC 50 concentration. However, concentrations below IC 50 had a less toxic effect, moreover, all concentrations that were used kept cell viability above 80%.
Garlic and Welsh onion extracts induced necrosis, according to the Annexin V/PI flow cytometry assay, with a very low rate of induced apoptosis. In Figure 8 it could be observed very clearly how both extracts reduced cell viability (dark green plots) and how intense is the necrosis (red plots), moreover the light blue plots indicate early apoptosis and light green plots late apoptosis, showing the massive difference between necrotic and apoptotic cell number, moreover, it indicates that the cell death morphological aspects and the elevated LDH activity at high concentrations of extracts were related to cell death by necrosis.

Antiproliferative Effect on Tumor Cells
Different concentrations of A. sativum and A. fistulosum extracts were investigated for their potential antiproliferative effect on four tumor cell lines, colorectal cancer cells (DLD-1), breast cancer cells (MDA-MB-231 and MCF-7), and lung carcinoma cells (SK-MES-1). For garlic hydroalcoholic extract, concentrations between 10% and 0.156% extract in cell culture medium were analyzed (Figure 9), while for Welsh onion extract the concentrations were between 2.5% and 0.156% (Figure 10), when adding 5% and 10% extract in the cell culture medium, we observed color change due to the pH indicator. We excluded those values from our analysis.
The IC50 for both Allium extracts were higher than 1.25% extract (equivalent to more than 1 mg/mL). A. sativum extract had IC50 values of 4.651% for SK-MES-1, which was the most sensitive cell line to garlic extract, 5.482% for DLD-1, 6.131% for MCF-7, and 6.375% for MDA-MB-231, which was the less sensitive cell line to garlic extract. Welsh onion extract had IC50 values of 2.124% for DLD-1 which is the most sensitive cell line to Welsh onion extract, 2.464% for MDA-MB-231, 3.353% for MCF-7, and 5.819% for SK-MES-1, which was the less sensitive cell line to Welsh onion. This value indicates that the extracts were concentrated in bioactive compounds, which might inhibit tumor cells, furthermore the extracts had a dose-dependent inhibitory effect on tumor cells. Alliin and allicin were the main compounds in both Allium extracts, the inhibitory effects were different and the inhibition rate was proportional with the concentration of extract added to the cells. The bioactive sulfuric compounds such as allicin and allyl sulfides have an antitumoral effect and are responsible for the growth inhibition of the studied tumor cells [4,[57][58][59]. For garlic hydroalcoholic extract, concentrations between 10% and 0.156% extract in cell culture medium were analyzed (Figure 9), while for Welsh onion extract the concentrations were between 2.5% and 0.156% (Figure 10), when adding 5% and 10% extract in the cell culture medium, we observed color change due to the pH indicator. We excluded those values from our analysis.
The IC 50 for both Allium extracts were higher than 1.25% extract (equivalent to more than 1 mg/mL). A. sativum extract had IC 50 values of 4.651% for SK-MES-1, which was the most sensitive cell line to garlic extract, 5.482% for DLD-1, 6.131% for MCF-7, and 6.375% for MDA-MB-231, which was the less sensitive cell line to garlic extract. Welsh onion extract had IC 50 values of 2.124% for DLD-1 which is the most sensitive cell line to Welsh onion extract, 2.464% for MDA-MB-231, 3.353% for MCF-7, and 5.819% for SK-MES-1, which was the less sensitive cell line to Welsh onion. This value indicates that the extracts were concentrated in bioactive compounds, which might inhibit tumor cells, furthermore the extracts had a dose-dependent inhibitory effect on tumor cells. Alliin and allicin were the main compounds in both Allium extracts, the inhibitory effects were different and the inhibition rate was proportional with the concentration of extract added to the cells. The bioactive sulfuric compounds such as allicin and allyl sulfides have an antitumoral effect and are responsible for the growth inhibition of the studied tumor cells [4,[57][58][59].
Allium extract used as a treatment, at a certain concentration can become cytotoxic for cells. At lower doses, no toxicity was observed on normal human cells. Allium extracts, particularly garlic, were investigated for their biological properties such as antioxidative [60,61], anti-inflammatory [62], antitumoral [63], antibacterial [64], and other biological properties due to their chemical composition.
The antiproliferative effect of A. sativum and A. fistulosum extracts was focused on active compounds within their composition and was highlighted in a dose-dependent manner. The results were expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001 with Student's t-test by comparison with control group (untreated). The samples treated with 10% extract in the culture medium (equivalent to 100 mg mL −1 ) showed highly significant inhibition, with a significant inhibitory effect even at 1% extract. SK-MES-1 was the most sensitive cell line with 4.651% extract as the IC50 value (equivalent to 46 mg/mL) and MDA-MB-231 the triple negative breast cancer cell line being the less sensitive cell line with 6.375% extract (equivalent to 63 mg/mL) as the IC50 value.
Allium extract used as a treatment, at a certain concentration can become cytotoxic for cells. At lower doses, no toxicity was observed on normal human cells. Allium extracts, particularly garlic, were investigated for their biological properties such as antioxidative [60,61], anti-inflammatory [62], antitumoral [63], antibacterial [64], and other biological properties due to their chemical composition.
The antiproliferative effect of A. sativum and A. fistulosum extracts was focused on active compounds within their composition and was highlighted in a dose-dependent manner.
It was demonstrated that both Allium extracts have an immunostimulatory effect [65] mostly due to their sulfur bioactive compounds, like allicin [19], being a possible therapeutical approach for alternative antitumor therapy. Further investigations and optimization of the therapeutic dose should be taken into consideration, moreover, a multidose experiment could present interest in investigating the antitumoral effect of Allium extracts and comparing the effects with their bioactive compounds and conventional therapeutical agents.
Allicin has the potential to play a role in antitumor therapies as lung, breast, and colorectal carcinoma [12,20]. Our previously published results indicate that the bioactive sulfuric compound can inhibit tumor cell proliferation, colony formation, and migration, moreover it can induce cell death by triggering apoptotic and non-apoptotic pathways [12]. In the last years, many researchers have demonstrated the in vitro antitumoral potential of allicin, which can inhibit human renal cell carcinoma via the HIF pathway [66], can inhibit thyroid cancer progression [67], can suppress migration and invasion in cervical cancer cells [68], or can sensitize hepatocellular cancer cells to the antitumor activity of cytostatic drugs like 5-fluorouracil [69]. The results were expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001 with Student's t-test by comparison with control group (untreated). The samples treated with 10% extract in the culture medium (equivalent to 100 mg mL −1 ) showed highly significant inhibition, with a significant inhibitory effect even at 1% extract. SK-MES-1 was the most sensitive cell line with 4.651% extract as the IC 50 value (equivalent to 46 mg/mL) and MDA-MB-231 the triple negative breast cancer cell line being the less sensitive cell line with 6.375% extract (equivalent to 63 mg/mL) as the IC 50 value. The results were expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001 with Student's t-test by comparison with the control group (untreated). Due to the pH changes induced by the 10% and 5% concentrations in the cell culture medium observed by the phenol red color change, the MTT assay was performed between 2.5% and 0.156% extract. The IC50 values were between 5.819% extract in cell culture medium for SK-MES-1 and 2.123% extract in cell culture medium for DLD-1 cell line.
A. sativum extract had 380 μg/mL allicin, while A. fistulosum had 20 μg/mL allicin. The antiproliferative effect of both extracts might be attributed to allicin, which was the main bioactive compound in Allium extracts. At the IC50 values, allicin was present at a concentration of 19 μg/mL equivalent for 5% A. sativum extract and at concentrations between 0.2 and 1 μg/mL equivalent for 2-5% A. fistulosum extract.
The antiproliferative effect of both Allium extracts was visible in a dose-dependent It was demonstrated that both Allium extracts have an immunostimulatory effect [65] mostly due to their sulfur bioactive compounds, like allicin [19], being a possible therapeutical approach for alternative antitumor therapy. Further investigations and optimization of the therapeutic dose should be taken into consideration, moreover, a multidose experiment could present interest in investigating the antitumoral effect of Allium extracts and comparing the effects with their bioactive compounds and conventional therapeutical agents.
Allicin has the potential to play a role in antitumor therapies as lung, breast, and colorectal carcinoma [12,20]. Our previously published results indicate that the bioactive sulfuric compound can inhibit tumor cell proliferation, colony formation, and migration, moreover it can induce cell death by triggering apoptotic and non-apoptotic pathways [12]. In the last years, many researchers have demonstrated the in vitro antitumoral potential of allicin, which can inhibit human renal cell carcinoma via the HIF pathway [66], can inhibit thyroid cancer progression [67], can suppress migration and invasion in cervical cancer cells [68], or can sensitize hepatocellular cancer cells to the antitumor activity of cytostatic drugs like 5-fluorouracil [69].
A. sativum extract had 380 µg/mL allicin, while A. fistulosum had 20 µg/mL allicin. The antiproliferative effect of both extracts might be attributed to allicin, which was the main bioactive compound in Allium extracts. At the IC 50 values, allicin was present at a concentration of 19 µg/mL equivalent for 5% A. sativum extract and at concentrations between 0.2 and 1 µg/mL equivalent for 2-5% A. fistulosum extract.
The antiproliferative effect of both Allium extracts was visible in a dose-dependent manner, depending on the concentration of the main bioactive compound, allicin. Other studies revealed that allicin has an antiproliferative effect against tumor cells at different concentrations, depending on the cell type. Allicin showed an inhibitory effect against U251 cells at an IC 50 of 41.97 µg/mL [70]; 10.38 µg/mL against SK-Hep-1 cells; and 10.00 µg/mL against BEL-7402 cells [69]. In our previous study, allicin inhibited SK-MES-1 cells at an IC 50 of 1.39 µg/mL (equivalent to 8.6 µM) and DLD-1 cells at IC 50 of 8.67 µg/mL (equivalent to 53.53 µM) [12]. Besides, allicin showed a synergistic effect when combined with 5-fluorouracil on DLD-1 and SK-MES-1 [12].
The antiproliferative effect of allicin from Allium extracts is completed by the phenolic compounds [2,18]. Moreover, glucoside quercetin has an important role in the antiproliferative effect of A. sativum, A. fistulosum, A. cepa, and A. chinense extracts against HepG2, PC-3, and HT-29 cell lines [69]. The dose-dependent inhibitory effect of A. sativum and A. fistulosum extracts is related to the concentration of allicin. Gruhlke et al. evaluated the inhibitory effect of allicin in garlic juice and synthesized allicin on lung adenocarcinoma (A549 cell line), human mammary carcinoma (MCF-7 cell line), human colorectal carcinoma (HT29 cell line), mouse fibroblasts (NIH 3T3 cell line), and human umbilical vein endothelial cells (HUVEC) and the results indicate an inhibitory rate on all cell lines, in a dose-dependent manner [20]. Furthermore, Allium extracts are rich in polyphenols, which play important role in the biological effects of garlic and Welsh onion. The antitumor effect of both Allium extracts is mainly attributed to allicin, while polyphenols are enhancing this biological effect. Fujimoto et al. demonstrated that polyphenols have a cytotoxic effect in a dose-dependent manner both on normal and tumor cells, and at a well-established concentration, polyphenols could inhibit tumor cell without affecting normal cells [71].
Polyphenols, in a dose-dependent manner, can induce cell death by inducing apoptosis [71]. Sulfuric compounds, like allicin, have a different effect on cell signaling pathways. Allicin inhibits cell growth and induces apoptosis via the ERK-dependent pathway [31] or can activate both intrinsic and extrinsic apoptotic pathways [25,33,70]. Moreover, allicin can inhibit tumor cell progression by suppressing the HIF pathway [66]. Allicin suppresses cervical cancer cells invasion and migration via inhibiting NRF 2 [68]. Furthermore, allicin has an immunostimulatory effect via Colec12, MARCO, and SCARB1 receptors [19]. A similar proapoptotic effect was observed in the case of diallyl trisulfides, which are part of Allium plants' chemical composition, Shin et al. demonstrated that diallyl trisulfides induce apoptosis in caspase-dependent manner and create cross-talks with PI3K/Akt and JNK pathways [58].
Both Allium extracts that were investigated in our study were rich in allicin and polyphenols and showed an inhibitory effect in a dose-dependent manner both on normal and tumor cells. At high concentrations, above the concentration obtained for the IC 50 , cell damage was induced and the LDH and CAT activity was increased. On the other hand, at lower concentrations the cellular stress was reduced, LDH and CAT activity were significantly decreased compared to the 10% extract. The inhibitory effect was related to the chemical composition of the extracts. The cytotoxicity could be reduced by choosing a therapeutic dose that is not toxic to normal cells and by isolating or synthesizing bioactive compounds from the extracts, compounds that are responsible for the biological effect of these plants.
Further investigations are needed to evaluate the therapeutical potential of Allium extracts and their bioactive compounds, focusing on the molecular links between the active compounds and the effector molecules in eukaryotic cells.

Plant Material and Growth Conditions
Garlic (Allium sativum L.) plants were cultivated in a private garden [10] and Welsh onion (Allium fistulosum L.) plants in the Botanical Garden "Alexandru Borza" from luj-Napoca, Romania. For each Allium species, a voucher specimen was deposited at the Herbarium of Babes-Bolyai University, Cluj-Napoca, Romania (Allium sativum L.-CL666161; Allium fistulosum L.-CL 669061).

Allium Extract Preparation
Different parts of the plants were used for the extract preparation, leaves for Allium fistulosum and bulbs for A. sativum. The cold repercolation method at room temperature for 3 days was used for preparing Allium plant extract [47] from small plant fragments (0.5-1 cm) and 70% ethanol (Merck, Bucharest, Romania).
The fluid extract (w:v/g:mL) obtained by filtration was 1:1.2 with 30% ethanol for A. fistulosum and 1:1 with 20% ethanol for A. sativum. Data regarding the chemical composition of A. sativum extract was previously published by Pârvu et al. [53].

Phytochemical Characterization of Extracts
The phytochemical characterization was performed according to the method that was previously published by Pârvu et al. [6]. Two HPLC-DAD-MS protocols were optimized to separate and determine the main phytoconstituents as allicin and alliin and polyphenolic compounds [3]. Total phenolic content (TPC) was determined using the Folin Ciocalteu reagent [72] and total tiosulfinate content (TTC) by a kinetic assay previously described [72,73].

MTT Assay on Normal and Tumor Cells
Cytotoxicity study of Allium extracts on normal and tumor cell lines were performed using MTT assay (Khazei et al., 2017). Cells were plated at 1 × 10 4 cells per well in a 96-well plate. After 24 h of incubation, cells were treated with A. sativum and A. fistulosum extract and incubated for 24 h at 37 • C in a humidified incubator with 5% CO 2 . After 24 h, the cell viability rate was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at 570 nm (Sigma-Aldrich, St. Louis, MI, USA). The plates were read with a TECAN SPARK10M (TECAN, Austria GmbH, Grodig, Austria).
The cell viability was calculated by the following equation: % Cytotoxicity = Absorbance of treated cells/Absorbance of negative control × 100. The 50% of the growth inhibition concentration (IC 50 ) was calculated from a plotted dose-response curve [36].

Confocal Microscopy-Morphological Analysis
Cells were processed following a triple staining protocol in order to highlight the mitochondrial networks, the cytoskeleton and the nucleus, using Mitotracker-Red, Phalloidin-FITC, and DAPI according to the protocol that was previously published by Budisan et al. [51]. Mitotracker (Thermo Fisher Scientific, Waltham, MS, USA) was added while the cells were still viable, after 90 min of incubation at 37 • C the cells were fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MI, USA) and the membranes were treated with 0.05% Triton X (Sigma-Aldrich, St. Louis, MI, USA). After Triton X treatment, Phalloidin-FITC (Cytoskeleton, Denver, CO, USA) was added and the samples were incubated for 30 min at room temperature. After Phalloidin incubation, 100 µM DAPI solution (Thermo Fisher Scientific, Waltham, MS, USA) was added for one minute. Between each step the cells were washed three times with phosphate saline buffer 1X (Gibco, Grand Island, NY, USA). Cells were analyzed under an Olympus FLUOVIEW FV1200 laser scanning fluorescence confocal microscope (Olympus, Tokyo, Japan).

Biochemical Determination of LDH, CAT, and Caspase 3
Cells were incubated at 1 × 10 5 cells per well in a 12-well plate at a final volume of 2000 µL. After 24 h incubation, cells were treated with extracts using three different concentrations (10% extract in the media, the IC 50 concentration and one concentration below IC 50 ). The supernatant was collected after 24 h incubation with extracts. To obtain the cell homogenate, 2000 µL of fresh media was added to each well, then the plates were frozen and thawed three times to dissociate the cell membranes, and the cell extract was collected for further analysis. Lactate-dehydrogenase (LDH) and catalase (CAT) [76], from supernatant samples were analyzed. LDH activity was measured at 340 nm using the LDH Activity Kit for in-vitro use (Biomaxima, Lublin, Poland) and the samples were read with a spectrophotometer (Biochemical Systems International, Model 2000 Evolution, Arezzo, Italy). Catalase activity was measured from the supernatant at 240 nm using an UV-Vis VWR UV-1600PC spectrophotometer (VWR, Radnor, PA, USA). Caspase 3 (Casp3) was determined from the cell supernatant using Human Caspase-3 (Activated) ELISA Kit from Invitrogen (Carlsbad, CA, USA) and the plate was read using a TECAN SPARK10M (TECAN, Austria GmbH, Grodig) according to the assay protocol and the results were calculated using Prism 8 software (San Diego, CA, USA).

Cell Death Investigation by Flow Cytometry
Cells were incubated at 5 × 10 4 cells per well in a 24-well plate at a final volume of 1500 µL. After 24 h incubation, cells were treated with extracts using three different concentrations (5% extract in the media, the IC 50 concentration, and one concentration below IC 50 ). For detaching cells, Trypsin-EDTA was used, and cells were washed three times with PBS1X, centrifuged and then the cells were resuspended in 200 µL of binding buffer. Annexin V-FITC (ExBIO, Prague, Czech Republic) and propidium iodide (PI) were added according to the manufacturer's protocol, 5 µL Annexin V and 5 µL of propidium iodide then 15 min of incubation at room temperature was performed to allow staining.
After incubation, cells were centrifuged and resuspended in 100 µL of 1X annexin binding buffer and the samples were immediately read using a FACS BD Flow cytometer (BD, San Jose, CA, USA) and the results were calculated with Prism8 software.

Statistical Analysis
Viability rate was calculated using Prism 8 software, two-way ANOVA, and Bonferroni's post-hoc test was considered statistically significant at p < 0.05 and was interpreted as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 when comparisons were made with the C group. All the normal cells were compared to an untreated group as the control and the results were expressed as the mean value ± SD. The results were expressed as * p < 0.05, ** p < 0.01, and *** p < 0.001 with Student's t-test by comparison with the control group (untreated) for the MTT assay on tumoral cells (GraphPad Software, San Diego, CA USA).

Conclusions
Allium sativum and A. fistulosum inhibited human fibroblasts and human keratinocytes growth when a high concentration of these extracts was applied. The MTT assay indicated a dose dependent growth inhibition for both extracts. Further observations of morphological changes in BJ and HaCaT cells, using a triple staining protocol, revealed the mitochondrial network, cytoskeleton, and nuclear modification, also the cell population was reduced in a dose dependent manner. Morphological modifications are indicators of the cell death induced by garlic and Welsh onion extracts at high doses of the extract. The flow cytometry analysis confirmed that cell death was induced at high extract concentrations, mostly by necrosis, correlating the results with the Casp3, LDH, and CAT analysis.
The extracts had different allicin concentrations, showing an antiproliferative effect against four tumor cell lines, with different IC 50 values, depending on the concentration of allicin according to the phytochemical analysis of both extracts. We believe that the use of isolated bioactive sulfuric compounds, such as allicin, therefore can be used against tumor cells with promising results. We aim to develop further in vivo studies focused on the molecular interaction of allicin and other bioactive compounds with key molecules that control biological processes in the cells to determine the therapeutical potential of Allium bioactive compounds