Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.


Introduction
Geum L., commonly called avens, is a genus in the Rosaceae family, subfamily Rosoideae, which comprises about 55 species of rhizome forming perennial herbaceous plants. The genus Geum is widespread across Eurasia, North and South America, and Africa. Avens species are found in exposed vegetation and forests and sometimes also as "weeds". The species G. montanum L. (alpine avens), G. reptans L. (creeping avens), G. urbanum L. (wood avens), and G. rivale L. (water avens) are found as part of the Central European flora. G. urbanum and G. rivale are among the best-known European species, which are also used in pharmaceutical applications, especially in complementary medicine such as phytotherapy. For this purpose, mainly the roots and rhizomes (in the following text summarized as roots), but also aerial parts of the two species are processed. G. urbanum and G. rivale have long been used in traditional European medicine for the treatment of diarrhea, stomach complaints, febrile diseases, gingivitis, and inflammation of mucous membranes [1]. In addition, the roots of wood avens and water avens have been used as a substitute for clove because of their eugenol content, as an additive to spirits and liqueurs in the food industry, and as cosmetic ingredients of toothpastes and mouthwashes [1]. The main secondary metabolites of the roots and herbal parts reported so far are galloand ellagitannins, procyanidins and other polyphenolics, ascorbic acid, and essential oil components, such as eugenol [2]. The high tannin content is typical for the Rosaceae family. More recent studies, especially on G. urbanum, have reported anti-inflammatory, antimicrobial, antioxidant, neuroprotective, and hypotensive effects [2]. It is, therefore, surprising that the phenolic compound and fatty-acid profiles of the seeds of these two species have neither been studied nor used for pharmaceutical, nutritional, or culinary purposes. Therefore, this study aimed at a profound characterization of the secondary metabolite profile of the seeds of G. urbanum and G. rivale, focusing particularly on phenolic compounds, such as monophenol structures and ellagitannins, as well as triterpenes and their derivatives. Furthermore, the fatty-acid profile was analyzed and compared between the two Geum species. In addition, two extraction methods, i.e., solvent extraction and cold-pressing, should be assessed with regard to seed oil yields and their compound profiles. Although Geum is known to be rich in phenolics, and extracts derived therefrom exert various pharmaceutical activities to treat several diseases, there is still a lack of comprehensive knowledge concerning the phytochemical composition of the seeds. The latter are expected to be potential sources of secondary metabolites, thus rendering them promising candidates for applications in the food, cosmetic, and pharmaceutical sectors.

GC/MS Analyses of Fatty Acids
The diaspores (fruit) of G. urbanum and G. rivale (Figure 1) are randomly spread by animals. For this reason, they have some morphologic adaptations such as spikes, hooks, barbed projections, or awns [3]. Furthermore, seeds store proteins, carbohydrates, phosphates, and lipids that act as the carbon skeleton and energy source, e.g., for germination [4]. To analyze the fatty-acid compositions, seeds were defatted with CH 2 Cl 2 or cold-pressed, and the oils obtained were analyzed by chromatographic and spectrometric methods. and essential oil components, such as eugenol [2]. The high tannin content is typical for the Rosaceae family. More recent studies, especially on G. urbanum, have reported antiinflammatory, antimicrobial, antioxidant, neuroprotective, and hypotensive effects [2]. It is, therefore, surprising that the phenolic compound and fatty-acid profiles of the seeds of these two species have neither been studied nor used for pharmaceutical, nutritional, or culinary purposes. Therefore, this study aimed at a profound characterization of the secondary metabolite profile of the seeds of G. urbanum and G. rivale, focusing particularly on phenolic compounds, such as monophenol structures and ellagitannins, as well as triterpenes and their derivatives. Furthermore, the fatty-acid profile was analyzed and compared between the two Geum species. In addition, two extraction methods, i.e., solvent extraction and cold-pressing, should be assessed with regard to seed oil yields and their compound profiles. Although Geum is known to be rich in phenolics, and extracts derived therefrom exert various pharmaceutical activities to treat several diseases, there is still a lack of comprehensive knowledge concerning the phytochemical composition of the seeds. The latter are expected to be potential sources of secondary metabolites, thus rendering them promising candidates for applications in the food, cosmetic, and pharmaceutical sectors.

GC/MS Analyses of Fatty Acids
The diaspores (fruit) of G. urbanum and G. rivale (Figure 1) are randomly spread by animals. For this reason, they have some morphologic adaptations such as spikes, hooks, barbed projections, or awns [3]. Furthermore, seeds store proteins, carbohydrates, phosphates, and lipids that act as the carbon skeleton and energy source, e.g., for germination [4]. To analyze the fatty-acid compositions, seeds were defatted with CH2Cl2 or cold-pressed, and the oils obtained were analyzed by chromatographic and spectrometric methods. The fatty-acid composition of the two species revealed the presence of palmitic acid, linoleic acid, α-linolenic acid, and stearic acid as major components, which were assigned on the basis of their mass spectra and a comparison with those of reference compounds and with the NIST database. The relative proportions of individual fatty acids (Figure 2) of the two species were almost identical for palmitic acid (G. urbanum: 5%; G. rivale: 4%) The fatty-acid composition of the two species revealed the presence of palmitic acid, linoleic acid, α-linolenic acid, and stearic acid as major components, which were assigned on the basis of their mass spectra and a comparison with those of reference compounds and with the NIST database. The relative proportions of individual fatty acids (Figure 2) of the two species were almost identical for palmitic acid (G. urbanum: 5%; G. rivale: 4%) and stearic acid (G. urbanum: 3%; G. rivale: 4%). In contrast, the proportions of linoleic acid (G. urbanum: 15%; G. rivale: 32%), α-linolenic acid (G. urbanum: 75%; G. rivale: 60%), and eicosanoic acid (G. urbanum: 2%; G. rivale: 0%) varied considerably. and stearic acid (G. urbanum: 3%; G. rivale: 4%). In contrast, the proportions of linoleic acid (G. urbanum: 15%; G. rivale: 32%), α-linolenic acid (G. urbanum: 75%; G. rivale: 60%), and eicosanoic acid (G. urbanum: 2%; G. rivale: 0%) varied considerably. The two Geum species differed in their relative amounts of C18:2, which was twice as high in G. rivale as compared to G. urbanum, whereas the former was devoid of C20:0. Furthermore, the two cold-pressed oils of G. urbanum and G. rivale seeds were compared in the same way ( Figure 2). The fatty-acid profile of the cold-pressed oils was almost identical to that of the oil samples resulting from CH2Cl2 extraction. The cold-pressed oil of G. urbanum revealed proportions of 4% palmitic acid, 16% linoleic acid, 77% linolenic acid, 2% stearic acid, and <1% eicosanoic acid, whereas the proportions for G. rivale were as follows: 5% palmitic acid, 47% linoleic acid, 45% linolenic acid, 3% stearic acid, and <1% eicosanoic acid. Interestingly, neither γ-linolenic acid (GLA; C18:3) nor oleic acid (C18:1) was detected in any of the samples according to a comparison with reference compounds. The high abundance of unsaturated fatty acids indicates a high-quality oil from a nutritional viewpoint. Furthermore, the cold-pressed oil of G. urbanum with its dark green color and high viscosity differed markedly from the oil obtained upon solvent extraction, which showed much lower viscosity. The latter parameter is significantly affected by the fatty-acid profile. Among other things, fatty acids can form crystalline structures, which have a marked impact on oil viscosity [5].

HPLC-DAD/MS n Analysis of Phenolic Compounds and Triterpenoids
In this study, MeOH extracts of G. urbanum and G. rivale seeds were studied in detail regarding their phenolic compound profile ( Figure 3). For this purpose, the seeds were defatted (CH2Cl2) and subsequently extracted with MeOH. Then, the methanolic extracts were subjected to analysis by HPLC-DAD/MS n to characterize individual phenolic compounds. Furthermore, the cold-pressed oils of both Geum species were extracted with MeOH and compared by LC/MS n . In summary, more than 100 individual compounds were characterized and tentatively assigned to these fractions on the basis of their retention times The two Geum species differed in their relative amounts of C18:2, which was twice as high in G. rivale as compared to G. urbanum, whereas the former was devoid of C20:0. Furthermore, the two cold-pressed oils of G. urbanum and G. rivale seeds were compared in the same way ( Figure 2). The fatty-acid profile of the cold-pressed oils was almost identical to that of the oil samples resulting from CH 2 Cl 2 extraction. The cold-pressed oil of G. urbanum revealed proportions of 4% palmitic acid, 16% linoleic acid, 77% linolenic acid, 2% stearic acid, and <1% eicosanoic acid, whereas the proportions for G. rivale were as follows: 5% palmitic acid, 47% linoleic acid, 45% linolenic acid, 3% stearic acid, and <1% eicosanoic acid. Interestingly, neither γ-linolenic acid (GLA; C18:3) nor oleic acid (C18:1) was detected in any of the samples according to a comparison with reference compounds. The high abundance of unsaturated fatty acids indicates a high-quality oil from a nutritional viewpoint. Furthermore, the cold-pressed oil of G. urbanum with its dark green color and high viscosity differed markedly from the oil obtained upon solvent extraction, which showed much lower viscosity. The latter parameter is significantly affected by the fattyacid profile. Among other things, fatty acids can form crystalline structures, which have a marked impact on oil viscosity [5].

HPLC-DAD/MS n Analysis of Phenolic Compounds and Triterpenoids
In this study, MeOH extracts of G. urbanum and G. rivale seeds were studied in detail regarding their phenolic compound profile ( Figure 3). For this purpose, the seeds were defatted (CH 2 Cl 2 ) and subsequently extracted with MeOH. Then, the methanolic extracts were subjected to analysis by HPLC-DAD/MS n to characterize individual phenolic compounds. Furthermore, the cold-pressed oils of both Geum species were extracted with MeOH and compared by LC/MS n . In summary, more than 100 individual compounds were characterized and tentatively assigned to these fractions on the basis of their retention times (t R ), UV/Vis spectra, mass-to-charge ratios (negative ionization mode), and their specific fragmentation patterns in comparison with bibliographic references (Table 1).
(tR), UV/Vis spectra, mass-to-charge ratios (negative ionization mode), and their specific fragmentation patterns in comparison with bibliographic references (Table 1).  Table 1. For the sake of clarity, not all assigned compounds are numbered. According to the retention times shown in Table 1, the corresponding peaks could be assigned.
The tentatively assigned compounds of the MeOH extracts of both species belonged to various classes of phenolics including hydroxybenzoic acid and hydroxycinnamic acid derivatives, flavonoids, and ellagitannins. Most of the detected phenolic constituents were characteristic of Rosaceae species rich in tannins. In plants, the biological function of these polyphenolics is mostly based on their protective capabilities against herbivores, pathogens, and UV-B radiation [6]. The main constituents are based on gallic acid core structures (Table 1), which are well known for the genus Geum. Moreover, some triterpenoids and their derivatives (tR = 61.9-78.6 min) could be tentatively assigned in Geum for the first time. The core structures of these triterpenoids belong to asiatic acid (AA) and madecassic acid (MA; Figure 4). The fragmentation patterns of 26 derivatives of AA and MA were compared with the reference standard of MA and literature data. Asiatic acid and its derivatives were first mentioned as secondary metabolites of Centella asiatica, an herbaceous, frost-susceptible perennial plant in the Apiaceae family [7]. AA is the most prominent constituent of Centella and possesses biological activities, notably anticancer, anti-inflammatory, wound healing, antidiabetic, antioxidant, hepatoprotective, anti-hepatitis C virus, and neuroprotective properties [7]. The triterpenoid derivatives of AA and MA were characterized in Geum for the first time. However, it was not possible to clearly assign all individual derivatives. The MA reference standard showed a peak cluster in a retention time range of tR = 67.8-70.0 min revealing fragment ions at m/z 503, 437, 407, 392, 363, and 159. In previous studies, 19 metabolites of AA and MA could be identified in addition to AA and MA with similar fragmentation patterns [8]. These were mainly formed by hydroxylation, dehydrogenation, dihydroxylation, and combinations of these reactions as a result of the metabolic capability of zebrafish (feeding study) [8].  Table 1. For the sake of clarity, not all assigned compounds are numbered. According to the retention times shown in Table 1, the corresponding peaks could be assigned.
The tentatively assigned compounds of the MeOH extracts of both species belonged to various classes of phenolics including hydroxybenzoic acid and hydroxycinnamic acid derivatives, flavonoids, and ellagitannins. Most of the detected phenolic constituents were characteristic of Rosaceae species rich in tannins. In plants, the biological function of these polyphenolics is mostly based on their protective capabilities against herbivores, pathogens, and UV-B radiation [6]. The main constituents are based on gallic acid core structures (Table 1), which are well known for the genus Geum. Moreover, some triterpenoids and their derivatives (t R = 61.9-78.6 min) could be tentatively assigned in Geum for the first time. The core structures of these triterpenoids belong to asiatic acid (AA) and madecassic acid (MA; Figure 4). The fragmentation patterns of 26 derivatives of AA and MA were compared with the reference standard of MA and literature data. Asiatic acid and its derivatives were first mentioned as secondary metabolites of Centella asiatica, an herbaceous, frost-susceptible perennial plant in the Apiaceae family [7]. AA is the most prominent constituent of Centella and possesses biological activities, notably anticancer, anti-inflammatory, wound healing, antidiabetic, antioxidant, hepatoprotective, anti-hepatitis C virus, and neuroprotective properties [7]. The triterpenoid derivatives of AA and MA were characterized in Geum for the first time. However, it was not possible to clearly assign all individual derivatives. The MA reference standard showed a peak cluster in a retention time range of t R = 67.8-70.0 min revealing fragment ions at m/z 503, 437, 407, 392, 363, and 159. In previous studies, 19 metabolites of AA and MA could be identified in addition to AA and MA with similar fragmentation patterns [8]. These were mainly formed by hydroxylation, dehydrogenation, dihydroxylation, and combinations of these reactions as a result of the metabolic capability of zebrafish (feeding study) [8]. Table 1. Peak assignment of metabolites detected in MeOH extracts of Geum urbanum (A: methanolic extract of defatted seeds; A*: methanolic extract of cold-pressed seed oil) and Geum rivale (B: methanolic extract of defatted seeds; B*: methanolic extract of cold-pressed seed oil) using HPLC-DAD/ESI-MS n (negative ionization mode). a For peak assignment, see Figure 3. Compounds without numbers were not characterized in the oily extracts A and B (Figure 3). b Putative assignment. c HHDP: hexahydroxydiphenic acid. d Exact assignment to one compound not possible. Upon comparison of the phenolic profiles (Table 1) of the previously defatted seeds with those of the cold-pressed oils, it becomes obvious that the latter were devoid of the numerous gallotannins (e.g., galloyl-HHDP-glucosides). In contrast, hydrolyzable tannins and further polar components were abundant in the methanolic seed extracts. The seed oils were characterized by the presence of flavonoids, phenolic acids, and triterpenoids. Furthermore, a lignan (pinoresinol derivative) and a sesquiterpene (abscisic acid derivative) were characterized. Previous studies of the roots and herbal parts of G. urbanum showed that the plant contains phenolic compounds (gallic acid, caffeic acid, chlorogenic acid, eugenol, flavonoids and tannins), vicianose and carotenoids [2,52,53]. Thus, more polar phenolic components of the seeds are partly or entirely discriminated upon oil recovery, presumably due to poor solubility in the fatty oil, and remain in the press cake. Ellagic acid derivatives were hardly present in the cold-pressed, light-green-colored seed oil of G. rivale. Furthermore, the cold-pressed oil of G. urbanum had a dark green color and was highly viscous. Since the extraction method can significantly influence the compound profile of an oil, a systematic comparison of different methods is of interest for future studies, including supercritical fluid extraction [54]. In contrast, the nonpolar bioactive triterpene derivatives of AA and MA were detected in the fatty oils of both species for the first time, which, in combination with the valuable fatty-acid profile, renders them promising sources for potential applications in the food, cosmetic, and pharmaceutical sectors. This favorable compound profile is attributed to the fact that triterpenoids may contribute to the bioactivity spectrum of fatty oils and that they may also have a structure-forming function similar to sterols, as postulated for olive oil [55-57]. Upon comparison of the phenolic profiles (Table 1) of the previously defatted seeds with those of the cold-pressed oils, it becomes obvious that the latter were devoid of the numerous gallotannins (e.g., galloyl-HHDP-glucosides). In contrast, hydrolyzable tannins and further polar components were abundant in the methanolic seed extracts. The seed oils were characterized by the presence of flavonoids, phenolic acids, and triterpenoids. Furthermore, a lignan (pinoresinol derivative) and a sesquiterpene (abscisic acid derivative) were characterized. Previous studies of the roots and herbal parts of G. urbanum showed that the plant contains phenolic compounds (gallic acid, caffeic acid, chlorogenic acid, eugenol, flavonoids and tannins), vicianose and carotenoids [2,52,53]. Thus, more polar phenolic components of the seeds are partly or entirely discriminated upon oil recovery, presumably due to poor solubility in the fatty oil, and remain in the press cake. Ellagic acid derivatives were hardly present in the cold-pressed, light-green-colored seed oil of G. rivale. Furthermore, the cold-pressed oil of G. urbanum had a dark green color and was highly viscous. Since the extraction method can significantly influence the compound profile of an oil, a systematic comparison of different methods is of interest for future studies, including supercritical fluid extraction [54]. In contrast, the nonpolar bioactive triterpene derivatives of AA and MA were detected in the fatty oils of both species for the first time, which, in combination with the valuable fatty-acid profile, renders them promising sources for potential applications in the food, cosmetic, and pharmaceutical sectors. This favorable compound profile is attributed to the fact that triterpenoids may contribute to the bioactivity spectrum of fatty oils and that they may also have a structure-forming function similar to sterols, as postulated for olive oil [55-57].

Determination of Total Phenolic Contents in Seed Oils and Assessment of their Antioxidant Potential
Estimation of total phenolic contents using the Folin-Ciocâlteu (FC) assay revealed that G. urbanum and G. rivale seed oils recovered by solvent extraction showed nearly the same phenolic contents ( Figure 5). These amounted to 0.091 ± 0 g gallic acid equivalents (GAE)/kg G. urbanum seed oil, whereas the oil of G. rivale seeds showed a phenolic content of 0.107 ± 0.003 g GAE/kg. Phenolic contents were also determined for the cold-pressed oils of both species revealing amounts of 0.488 ± 0.016 g GAE/kg (G. urbanum) and 0.080 ± 0 g GAE/kg (G. rivale), respectively. The difference in the phenolic contents of G. urbanum seed oil of 0.091 g/kg GAE (extraction) and 0.488 g/kg GAE (cold-pressing) is supposedly due to the difference of the extraction methods. Extraction with CH2Cl2 appeared to enhance the proportions of phenolics, such as HHDP derivatives, which are characterized by lower antioxidant capacity as determined by the FC assay. The difference between the two measured values of 0.107 g/kg GAE and 0.080 g/kg GAE of G. rivale seed oils are probably within the range of the natural variation of phenolic contents or result from the different extraction methods.
It must be kept in mind that oil recovery by cold-pressing and solvent extraction may significantly differ with regard to the resulting compound profile and contents. For comparison, a study on extra virgin olive oil (EVOO) showed total phenolic contents to range from 0.138 to 0.278 g GAE/kg [58]. EVOOs are known for their abundance of phenolic compounds. Oils obtained by extraction and pressing may significantly differ in their metabolite profiles. Based on its physicochemical traits, CH2Cl2 selectively extracts nonpolar and moderately polar seed components. This may result in the discrimination of phenolics, thereby lowering the total phenolic contents in the extracted oil. Thus, for a true comparison, Geum seeds were also pressed to evaluate the phenolic contents of the respective seed oil. Phenolic compounds have a significant impact on the stability, sensory, and nutritional characteristics of plant-based products and may prevent deterioration through quenching of radical reactions responsible for lipid oxidation [59,60]. Such antioxidant effects may be assessed using the FC assay, which also indicates the antioxidant phenolic content of a sample. In particular, the cold-pressed seed oil of G. urbanum with its high The difference in the phenolic contents of G. urbanum seed oil of 0.091 g/kg GAE (extraction) and 0.488 g/kg GAE (cold-pressing) is supposedly due to the difference of the extraction methods. Extraction with CH 2 Cl 2 appeared to enhance the proportions of phenolics, such as HHDP derivatives, which are characterized by lower antioxidant capacity as determined by the FC assay. The difference between the two measured values of 0.107 g/kg GAE and 0.080 g/kg GAE of G. rivale seed oils are probably within the range of the natural variation of phenolic contents or result from the different extraction methods.
It must be kept in mind that oil recovery by cold-pressing and solvent extraction may significantly differ with regard to the resulting compound profile and contents. For comparison, a study on extra virgin olive oil (EVOO) showed total phenolic contents to range from 0.138 to 0.278 g GAE/kg [58]. EVOOs are known for their abundance of phenolic compounds. Oils obtained by extraction and pressing may significantly differ in their metabolite profiles. Based on its physicochemical traits, CH 2 Cl 2 selectively extracts nonpolar and moderately polar seed components. This may result in the discrimination of phenolics, thereby lowering the total phenolic contents in the extracted oil. Thus, for a true comparison, Geum seeds were also pressed to evaluate the phenolic contents of the respective seed oil. Phenolic compounds have a significant impact on the stability, sensory, and nutritional characteristics of plant-based products and may prevent deterioration through quenching of radical reactions responsible for lipid oxidation [59,60]. Such antioxidant effects may be assessed using the FC assay, which also indicates the antioxidant phenolic content of a sample. In particular, the cold-pressed seed oil of G. urbanum with its high phenolic content (0.488 g GAE/kg) may be promising from a nutritional viewpoint, as well as a high-quality oil for cosmetic purposes. Furthermore, previous studies reported total phenolic acid content (2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, DPPH) of 0.768 ± 0.026 g GAE/kg (768.2 ± 25.9 mg GAE/L) of aqueous G. urbanum root extracts [61]. Other investigations of dried arial and underground parts of G. rivale reported phenolic contents nearly twice as high in the underground parts (17.48% GAE) compared to other plant materials tested (aerial parts: 7.83% GAE (G. rivale), 7.61% GAE (G. urbanum); underground parts: 7.89% GAE (G. urbanum)) with FC [62]. The high phenolic content and associated endogenous antioxidant activity of G. urbanum seed oil in the Folin-Ciocâlteu assay is presumably due to the presence of ellagic acid and its derivatives. Thus, future studies will have to focus on the natural variability of seed phenolic contents and profiles of Geum species from different origins.

Plant Material
Seeds of G. urbanum L. and G. rivale L. were acquired from Jelitto Perenial Seeds GmbH (Schwarmstedt, Germany). Both species were identified by Dr. R. Duque-Thüs, and voucher specimens (voucher numbers: HOH-022758, HOH-022834 (G. urbanum); HOH-022759, HOH-022835 (G. rivale)) were deposited at the herbarium of the Institute of Botany at Hohenheim University (Stuttgart, Germany). In total, 520 g of Geum seeds were processed for all analyses performed in this work.

Extraction of Geum Seeds
Seeds of G. urbanum and G. rivale (20.0 g each) were separately immersed in 180 mL of CH 2 Cl 2 , and each batch was minced by Ultra-turrax ® treatment (2 min; 17,000 rpm, IKA Werke GmbH & Co. KG, Staufen, Germany), under external ice cooling. After maceration over night at 4 • C, the seeds were filtered off over Celite by vacuum suction and extracted a second time in the same manner (overnight). Two oil fractions were recovered from the combined CH 2 Cl 2 extracts by vacuum rotoevaporation of the solvent (oily extracts). Subsequently, the defatted seeds were extracted twice with MeOH (2 × 180 mL each) and filtered off; the extracts were combined and MeOH was removed in vacuo (rotary evaporation).
In a second variant, oil was recovered using an oil press (Rommelsbacher OP 700 Emilio, ROMMELSBACHER ElektroHausgeräte GmbH, Germany). For this purpose, 100 g of G. rivale seeds and 100 g + 80 g of G. urbanum seeds were cold-pressed (T < 40 • C). The yielded oil crude extracts were centrifuged (10 min, 1000 rpm) to yield clear oil samples. (since G. urbanum seeds appeared to be very dry, another amount of 80 g together with the press residue of previously pressed seeds (100 g) was ground in the oil press for oil recovery). For phenolic compound analysis, aliquots of 2.5 g oil were dissolved in 5 mL of hexane and subsequently extracted two times with 5 mL of CH 3 OH-H 2 O (80:20, v/v) by 2 min vortex treatment and 5 min of centrifugation (4500 rpm). The methanol phases were removed by rotary evaporation, and the residues were dissolved in 1 mL MeOH/H 2 O (1/1; v/v) prior to HPLC-DAD/MS n analyses. Each measurement was replicated three times (n = 3).

Methylation of Fatty Acids for GC/MS Analyses
Fatty-acid methyl esters (FAME) were prepared by column derivatization with trimethylsulfonium hydroxide (TMSH, 0.25 M in MeOH). Briefly, 10 mg of viscous oil sample (residue of CH 2 Cl 2 extraction) was dissolved in 2000 µL of TBME. Aliquots of 10 µL of this test solution were mixed with 170 µL of TBME followed by 60 µL of TMSH [63]. Subsequently, the mixture was directly injected into the GC system (n =3).

Folin-Ciocâlteu Assay for Total Phenolic Content Determination and Assessment of Antioxidant Capacity
Total phenolic contents of plant extracts were determined employing the Folin-Ciocâlteu (FC) assay with gallic acid as standard [64]. For the analysis of oil samples, the method was adapted as follows: 2.5 g oil was diluted with 5 mL of n-hexane, and this solution was extracted two times with 6 mL of CH 3 OH-H 2 O (80:20, v/v) by 2 min vortex treatment and 5 min of centrifugation (4500 rpm). These extracts were analyzed in triplicate according to the following procedure: a portion of 1 mL of the extract was added to 0.25 mL of FC reagent (2 N) in a 10 mL volumetric flask. After 3 min at room temperature, 1.5 mL of Na 2 CO 3 solution (20%, w/v) was added and mixed, and the volumetric flask was made up with purified water to the final volume. The samples were stored for 1 h at room temperature and centrifuged for 10 min at 12,000 rpm. Afterward, spectrometric analyses of the clear supernatant were performed at λ = 725 nm. Each measurement was replicated three times. The result, expressed in grams of gallic acid equivalents/kg, was calculated using a calibration curve established in a range of 0.05 to 0.17 mg/mL (concentrations: 0.05, 0.075, 0.1, 0.15, and 0.17 mg/mL). The standard curve of gallic acid was y = 4.5032x + 0.0574 with R 2 = 0.9986 for the extracted seed oil samples (n = 3). The standard curve of gallic acid for the cold-pressed seed oil samples was y = 4.6544x + 0.0446 with R 2 = 0.9992 (n = 4).

GC/MS Analyses of Seed Extracts after Derivatization
GC/MS analyses were performed with a PerkinElmer Clarus 500 gas chromatograph (PerkinElmer, Inc., Shelton, CT, USA) with split injection (split ratio 30:1, injection volume 1.0 µL), coupled to a single quadrupole mass detector. The column used was a Zebron ZB-5MS capillary column (60 m × 0.25 mm i.d. × 0.25 µm film thickness, 5% phenylpolysiloxane and 95% dimethylpolysiloxane coating; Phenomenex, Torrance, CA, USA). Carrier gas was helium at a flow rate of 1 mL/min. The injector used was a PSS (temperature-programmed split/splitless injector, temperature: 250 • C). The temperature program for the column oven was 100-320 • C with a linear gradient of 4 • C/min and a final hold time of 30 min. The mass spectrometer was run in electron ionization (EI) mode (70 eV). The software Turbomass (v.5.4.2, PerkinElmer Inc., Boston, MA, USA) was used for data acquisition and processing [65].

HPLC-(DAD)/ESI-MS n Analyses of Phenolic Compounds
Liquid chromatographic analyses were carried out on an Agilent 1200 HPLC system (Agilent Technologies, Inc., Palo Alto, CA, USA), equipped with a binary pump, a micro vacuum degasser, an autosampler, a thermostatic column compartment, and a UV/Vis diode array detector. An HCTultra ion trap mass spectrometer (Bruker Daltonik GmbH, Bremen, Germany) with an ESI source operating in the negative ionization mode was coupled to the LC system, applying the following parameters: capillary voltage: +4000 V, dry gas (N 2 ) flow rate: 9.00 L/min with a capillary temperature of 365 • C; nebulizer pressure: 50 psi. Full-scan mass spectra (mass range m/z 50-1300) of HPLC eluates were recorded during chromatographic separation yielding [M − H] − ions. MS n data were acquired in the auto MS/MS mode. The instruments were controlled by Agilent Chemstation and Esquire-Control software (V7.1). A Kinetex ® C18 reversed-phase column (2.6 µm particle size, 150 × 2.1 mm i.d., Phenomenex Ltd., Aschaffenburg, Germany) was used for chromatographic separation at 25 • C at a flow rate of 0.21 mL/min. The mobile phase consisted of HCOOH/H 2 O, 0.1/99.9 (v/v; eluent A) and MeCN (mobile phase B). The injection volume of each sample was 10 µL, and the gradient used was as follows:

Conclusions
This study reports, for the first time, the recovery of fatty oils from the seeds of G. urbanum and G. rivale and provides an in-depth analysis of the major constituents. In summary, an unsaturated oil with potentially biologically active phenolics may be recovered from the seeds. In particular, ellagic acid and HHDP derivatives were characterized in the solvent-extracted oils, which were also characterized in roots and herbal parts of these species in previous studies. In contrast, the latter compounds were only found at low concentrations in the cold-pressed oils. These oils are also particularly interesting due to the occurrence of triterpenoid derivatives of asiatic and madecassic acid, which have promising biological activities, such as anti-inflammatory, wound healing, and anticancer properties. In particular, the antioxidant, antibacterial, antifungal, and anti-inflammatory properties of the oils and the corresponding active principles should be investigated in more detail in future studies. Thus, the present study with its characterization of secondary metabolites provides a first step indicating the seed oils of Geum species as having a promising bioactivity profile.