TREK-TRAAK two-pore domain potassium channels protect human retinal pigment epithelium cells from oxidative stress

The aim of the current study was to explore the potential of TREK-TRAAK two-pore domain potassium (K2P) channels in protecting human retinal pigment epithelium (hRPE) cells against oxidative stress. hRPE cells were obtained from donors, and then cell identification and detection of the expression levels of TREK-TRAAK K2P channels in hRPE cells were conducted. Subsequently, tert-butyl hydroperoxide (t-BH) was used to induce oxidative stress in hRPE cells. Docosahexaenoic acid (DHA) was used to stimulate and fluoxetine was used to inhibit the TREK-TRAAK K2P channels. The survival rates of hRPE cells under oxidative stress were examined using flow cytometry. Apoptosis-associated factors, including Bax, Bcl-2, cleaved-caspase-3, αB-crystallin and their mRNAs, were examined using immunofluorescence, western blot and reverse transcription-polymerase chain reaction analyses. Variations in the cytoarchitecture were observed by immunofluorescence and electron microscopy. The cells examined in the present study were identified as hRPE cells. All members in the TREK-TRAAK K2P channel family (including TREK-1, TREK-2 and TRAAK) were found to be expressed in hRPE cells. Stimulation of TREK-TRAAK K2P channels increased the survival rates of hRPE cells under oxidative stress and the levels of intracellular protective factors, such as Bcl-2 and αB-crystallin. By contrast, inhibition of these channels decreased the cell survival rates and increased apoptosis enhancing factors, such as Bax and cleaved-caspase-3. Further examination of the cytoarchitecture revealed that TREK-TRAAK K2P channels protected the integrity of the hRPE cell structure against oxidative stress. In conclusion, the present study suggested that the activated TREK-TRAAK K2P channels serve a role in protecting hRPE cells against the oxidative stress induced by t-BH, which indicated that these K2P channels are potential novel targets in retinal protection and provided a new direction for research and therapy in retinal degeneration diseases.


Introduction
Retinal degeneration diseases, such as age-related macular degeneration (AMd) and retinitis pigmentosa (RP), threaten the health and quality of life in humans (1). Retinal pigment epithelium (RPE) cells are involved in these diseases (2). The majority of pathways contributing to cell death share a common ultimate pathway, namely the regulation of caspase signaling. However, the existing inhibitors of this pathway display no capability of completely stopping the apoptotic process, indicating that the identification of efficient upstream targets that regulate apoptosis is necessary (3,4).
Weakly inward rectifying potassium channels, including TREK and TRAAK, are members of the two-pore domain potassium (K2P) channels and are mainly found in the central nervous system (cNS). They contribute to the background (leak) potassium currents and regulate the viability of cells (5,6). Previous studies have focused on the functions and mechanism of the channels, such as their roles in cNS degenerative disease processes, synaptic transmission, the maintenance of membrane resting potential and cell volume regulation (7)(8)(9). In degenerative processes of ischemia-induced neurons, the TRAAK channels demonstrate the ability to downregulate neuronal excitability that leads to lower cellular metabolic rate (10)(11)(12).
The retina, as a direct extension of the cNS, possesses physiological structures and functions resembling those of the encephalon. Its degeneration mechanism and performance have several properties that are similar to those in the cNS. Thus, it is hypothesized that the TRAAK K2P would also be involved in the inhibition of retinal cell apoptosis. The activity of these channels is regulated by mechanical stretch, polyunsaturated fatty acids (PUFAs), different drugs, extracellular pH and osmotic pressure among others (13). docosahexaenoic acid (dHA) is one of the PUFAs that has been reported to be an activator of these channels and important in maintaining the integrity of RPE cells and photoreceptors (14). Several pharmacological agents, such as fluoxetine, inhibit the activity of these channels. Fluoxetine has also been reported to strongly suppress the PUFA-mediated currents of TREK-TRAAK K2P channels (15)(16)(17). Therefore, the present study investigated the underlying mechanism of these channels using dHA as a stimulator, while utilizing fluoxetine as an inhibitor.
In our previous studies, it was observed that riluzole (also known as 2-amino-6-trifluoromethoxy benzothiazole), a neuroprotective drug and activator of TRAAK channels, exhibited the ability to protect human RPE (hRPE) cells against oxidative injury-induced cell death (18) through stabilizing the mitochondrial membrane potential and preventing the release of cytochrome c (19). In order to better evaluate the effects of the these channels against apoptosis, the present study used dHA to stimulate this channel, while utilizing fluoxetine as a TREK-TRAAK K2P inhibitor.

Materials and methods
Cell extraction and identification. The present study was conducted in accordance with the declaration of Helsinki and approved by the Medical Ethics committee of Zhongshan Ophthalmic center, Sun Yat-sen University (Guangzhou, china). Written informed consent was obtained for all study participants. A total of 8 bulbus oculi were obtained from 5 adult donors, all of whom succumbed following traffic collisions (median age, 26; age range 19-40; 3 males, 2 females; collected between January 2014 and October 2015). HRPE cells were extracted from 8 eyecups of these bulbus oculi subsequent to the application of corneal transplantation, performed at the State Key Laboratory of Ophthalmology (Guangzhou, china). The cell culture, storage and subculture were performed following previously described guidelines (20). Subsequent experiments were performed on the fourth generation of hRPE cells, when the cells had reached the optimum level of cell viability and morphology, in which the cell confluence rate reached between 70 and 90%. Cell identification was conducted using immunofluorescent staining of RPE-specific 65 kDa protein (RPE65) and pan cytokeratin antibodies as described below.
Cell culture and subgroups. The hRPE cells were divided into following groups: control group, model group, channel-activated group and channel-inhibited group. cells were first cultured in medium comprising 90% DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 10% fetal bovine serum (Sijiqing, Zhejiang Tianhang Biotechnology co., Ltd.; Hangzhou, Zhejiang, china), at 25˚C. In the model group (t-BH group), the medium was removed and replaced with medium containing 300 µmol/l t-BH (Sigma-Aldrich; Merck KGaA, darmstadt, Germany) for 3 and 6 h in order to achieve the desired cell death. In the channel-activated group, 10 µmol/l dHA (Sigma-Aldrich; Merck KGaA) was used to stimulate the TREK-TRAAK K2P channels. Briefly, hRPE cells were pre-cultured in medium containing 10 µmol/l dHA for 24 h before the replacement of medium containing 300 µmol/l t-BH. In the channel-inhibited group, fluoxetine (Sigma-Aldrich; Merck KGaA) was used to block the TREK-TRAAK K2P channels by adding into the medium together with 10 µmol/l DHA. Briefly, in this group, hRPE cells were co-cultured in medium containing 10 µmol/l dHA and 70 µmol/l fluoxetine for 24 h before inducing oxidative stress by culturing the cells in medium containing 300 µmol/l t-BH in the model group, channel-activated group and channel-inhibited group.
Flow cytometry. The survival rate of hRPE cells was tested using the flow cytometry technique with an Annexin V/PI apoptosis detection kit (Bd Biosciences, Franklin Lakes, NJ, USA). The experiment was conducted in accordance with the protocol described in the kit. The cell quantity of each sample was adjusted to 10 5 cells/ml prior to testing.
Immunofluorescence assay. The expression levels of TREK-TRAAK channel proteins in hRPE cells were tested by immunofluorescence assay. The variations in apoptosis-associated factors were examined, with Bax and Bcl-2 levels measured in groups treated with t-BH for 3 h, and cleaved-caspase-3 and αB-crystallin levels tested in groups treated with t-BH for 3 and 6 h. In addition, F-actin and α-Tubulin levels were measured to observe the variations in the cytoskeleton in groups treated with t-BH for 3 and 6 h.
Briefly, the cells were fixed with 4% paraformaldehyde for 15 min under 4˚C, permeabilized with 0.1% Triton X-100-PBS for 5 min, and blocked in goat serum for 1 h at 25˚C. Next, the cells were subjected to overnight incubation with the primary antibody at 4˚C. After washing three times in PBS, the cells were incubated with secondary antibodies for another 30 min at 25˚C in the dark. Subsequent to washing in PBS, cell nuclei were separately counterstained with Reddot1 (Biotium, Fremont, CA, USA) for cell identification or with Hoechst 33342 (Beyotime Institute of Biotechnology, Haimen, china) for immunofluorescence within 10 min. detection was then performed using a fluorescence microscope (BX53; Olympus corporation, Tokyo, Japan), and the images were analyzed with image-pro plus 6 Western blot analysis. Variations in the levels of apoptosis-associated factors, including Bax, Bcl-2, cleaved-caspase-3 and αB-crystallin, were assessed by western blot assay in groups treated with t-BH for 3 and 6 h. Briefly, hRPE cells in the different groups were washed three times with ice-cold PBS and lysed in radioimmunoprecipitation assay buffer (Beyotime Institute of Biotechnology). The lysates were then centrifuged for 10 min under 12,000 x g at 4˚C, followed by electrophoresis at 75 V in 15% SdS-PAGE separation gel, then the proteins were electrotransferred at 300 mA for 30 min to polyvinylidene difluoride membranes with a 0.2 µm pore size (cat. 88520; Invitrogen; Thermo Fisher Scientific, Inc.). The membranes were subsequently blocked using 5% bovine serum albumin (Sigma-Aldrich; Merck KGaA) at 25˚C for 1 h. Following incubation overnight with the primary antibody solutions under 4˚C, the membranes were washed three times in PBS and then incubated with the secondary antibodies for another 30 min at 25˚C. Detection was then performed using Pierce™ EcL western blotting substrate (cat. 32106; Invitrogen; Thermo Fisher Scientific, Inc.) and analyzed with image-pro plus (version 6.0; Media cybernetics; Md, USA). The primary and secondary antibodies used in this assay were the following: Mouse anti-Bax (1:1,000; cat. ab77566; Abcam), rabbit anti-Bcl-2 (1:1,000; cat. ab32124; Abcam), rabbit anti-cleaved-caspase-3 (1:1,000; cat. SAB1305630; Sigma-Aldrich; Merck KGaA), rabbit anti-αB-crystallin (1:1,000; cat. SAB4500486; Sigma-Aldrich; Merck KGaA), horse anti-mouse horseradish peroxidase (HRP)-conjugated IgG (1:3,000; cat. #7076; cell Signaling Technology, Inc.) and goat anti-rabbit HRP-conjugated IgG (1:3,000; cat. #7074; cell Signaling Technology, Inc.).

Reverse transcription-polymerase chain reaction (RT-PCR).
RT-PcR analysis was performed to detect early variations in the Bax, Bcl-2, caspase-3 and αB-crystallin mRNA levels only in groups treated with t-BH for 3 h. Briefly, cells in different groups were washed three times with ice-cold PBS, lysed in TRIzol reagent buffer (Invitrogen; Thermo Fisher Scientific, Inc.) and then centrifuged for 8 min under 13,000 x g at 4˚C. RNA concentration was measured using a nano drop (Thermo Fisher Scientific, Inc.). Total RNA was reverse transcribed using the RevertAid First Strand cdNA Synthesis kit (Thermo Fisher Scientific, Inc.), and then FastStart Universal SYBR Green Master (Roche diagnostics GmbH, Mannheim, Germany) and specific primers were used for the PCR process. The thermocycling conditions were as follows: Stage 1, 42˚C for 5 min, 95˚C for 10 sec for 1 cycle; stage 2, 95˚C for 5 sec, 60˚C for 30 sec for 40 cycles; stage 3 (dissociation protocol), 95˚C for 1 sec, 65˚C for 15 sec. Results were quantified using a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, Inc.). The quantification cycle (cq) number was set by the StepOne Software (version 2.3; Thermo Fisher Scientific, Inc.). The expression levels of the tested mRNA were presented as the cq compared with the β-actin mRNA in each sample (21). The primer sequences were as follows: β-actin, forward, cAc cc AGc AcA ATG AAG ATc AAG AT and reverse, cAG TTT TTA AAT ccT GAG TcA AGc; caspase3, forward, TGG AAG cGA ATc AAT GGA cTc T and reverse, TGA ATG TTT ccc TGA GGT TTG c; αB-crystallin, forward, cGc cTc TTT GAc cAG TTc TTc and reverse, cTc AAT cAc ATc Tcc cAA cAc cT; bax, forward, TTT TGc TTc AGG GTT TcA Tcc A and reverse, TGc cAc TcG GAA AAA GAc cTc; bcl2, forward, ATc Gcc cTG TGG ATG AcT GA and reverse, GAG AcA Gcc AGG AGA AAT cAA Ac.
Electron microscopy. Late-stage variations in the cytoarchitecture were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in groups which treated with t-BH for 6 h. Briefly, cells were fixed with 2.5% pentanediol and 4% paraformaldehyde for 24 h at 4˚C, subjected to four-step dehydration with ethanol at 10, 30, 70 and 99% for 30 min each at room temperature, then coated with silver using Leica EM Sample Preparation (Genmany) for SEM and embedded with epoxy resin (cat. GMS11012; Genmed, Shanghai, china) for TEM. Observation of the cell samples by SEM and TEM was then performed.
Statistical analysis. data are expressed as the mean ± standard deviation. differences between groups were assessed with one-way analysis of variance. Statistical analysis was conducted with SPSS statistical software (version 13.0; SPSS, Inc., chicago, IL, USA). A value of P<0.05 was considered as indicative of a statistical significance difference.

TREK-TRAAK K2P channels are expressed in hRPE cells.
TREK-TRAAK K2P channels have been reported to be predominantly expressed in the human cNS (13); however, they have not been studied in hRPE cells. In the present study, the extracted cells were identified to be hRPE cells via immunofluorescence ( Figs. 1 and 2). Furthermore, expression of all three members of the TREK-TRAAK K2P channel family, including TREK-1, TREK-2 and TRAAK, was observed in the hRPE cells, with diffused distribution detected across the surface of hRPE cells (Fig. 3), and the spatial expression and quantity of the three members' protein exhibited no difference.
TREK-TRAAK K2P channels protect hRPE cells from oxidative stress. t-BH induces oxidative stress in hRPE cells and causes cell death. In the current study, the TREK-TRAAK K2P channels in hRPE cells were stimulated using dHA, while this stimulation was blocked by co-treated with fluoxetine. Evaluation of the protective effects of the channels was conducted using Annexin V/PI staining and flow cytometry (Fig. 4). Following exposure to 300 µmol/l t-BH for 3 h, the survival rate of hRPE cells was significantly decreased from 98.7±0.2 to 77.7±3.0%. The use of dHA raised the survival rate to 89.8±0.6%, while co-treatment with fluoxetine markedly reduced the survival rate to 65.4±2.6%. Similar results were obtained when the exposure was prolonged to 6 h. The survival rate of hRPE cells treated with 300 µmol/l t-BH for 6 h was significantly decreased to 50.8±3.0%, while the survival rate was 82.8±1.5% in the group treated with dHA and 45.5±0.7% in the group co-treated with fluoxetine.
The variations in the survival rates indicated that the TREK-TRAAK K2P channels served a protective function against oxidation in hRPE cells. Stimulating the TREK-TRAAK K2P channels resulted in higher cell survival rates under oxidative stress, whereas blocking the channels led to lower survival rates of hRPE cells.
TREK-TRAAK K2P channels regulate several apoptosisassociated factors in hRPE cells. Using immunofluorescence (Figs. 5 and 6) and western blot analysis (Fig. 7), it was observed that 3-h treatment with 300 µmol/l t-BH greatly increased the amount of Bax in hRPE cells under oxidative stress, while Bcl-2 expression was decreased. Introduction of 10 µmol/l dHA for 24 h prior to t-BH exposure inhibited the variation in these factors, reducing the increase in Bax and the decrease in Bcl-2 expression. However, co-treatment with 70 µmol/l fluoxetine for 24 h alongside DHA reversed this inhibition. Similar changes were identified for cleaved-caspase-3 expression. Following treatment with 300 µmol/l t-BH for 3 and 6 h, the levels of cleaved-caspase-3 were significantly enhanced.  dHA was found to restrain the change in cleaved-caspase-3, while fluoxetine was able to neutralize the function of DHA. Regarding αB-crystallin expression, the variations were slightly different (Figs. 6 and 7). Oxidative stress raised the levels of αB-crystallin after 3-h treatment with 300 µmol/l t-BH, while it caused αB-crystallin reduction in the 6-h group. Furthermore, pretreatment with dHA increased the αB-crystallin levels, whereas fluoxetine depressed the amount of αB-crystallin.
Using RT-PcR (Fig. 8), the early variations in Bax, Bcl-2, caspase-3 and αB-crystallin mRNA levels were observed in the group treated with 300 µmol/l t-BH for 3 h. Since the quantity of mRNA is altered prior to the change in protein levels, treatment for 3 h served as an observational point. consistently, the transcription levels of Bax and caspase-3 were enhanced after treatment with t-BH, while that of Bcl-2 was decreased and a moderate rise was observed in αB-crystallin level. dHA was found to inhibit the transcription levels of Bax and caspase-3, and to promote Bcl-2 and αB-crystallin. By contrast, fluoxetine treatment blocked the effect of dHA in this experiment. However, Bax in the group co-treated with fluoxetine was lower than expected, which may have resulted from severe oxidation inducing transcriptional function damage.
TREK-TRAAK K2P channels maintain the stabilization of the cytoarchitecture in hRPE cells. Immunofluorescence was used to detect the transformations of the cytoskeleton in hRPE cells under oxidative stress (Fig. 9). Following treatment with 300 µmol/l t-BH for 3 h, F-actin and α-Tubulin were initially depolymerized, and partially dissolved. However, in the dHA group, transformation was not detected. In the fluoxetine co-treatment group, F-actin and α-Tubulin were further disintegrated. In the group treated with 300 µmol/l t-BH for 6 h, the natural framework of the cytoskeleton was hardly detected. However, dHA pretreatment helped maintain the majority of the normal cell structure, though the framework was nebulous in certain parts. By contrast, the cytoskeleton of cells in the fluoxetine group was worse compared with that in the t-BH group. Furthermore, immunofluorescence assay identified that the nuclei of hRPE cells in the t-BH group exhibited atrophy and apoptotic bodies appeared. In the dHA group, the atrophy was alleviated and apoptotic bodies were rarely observed, while deterioration of these features was observed in cells co-treated with fluoxetine (Fig. 9).
SEM was applied to examine the extracellular variations of the cytoarchitecture (Fig. 10). Treatment with 300 µmol/l t-BH for 6 h led to atrophy and permeabilization of the cell membrane. compared with the control, microvilli and pseudopodia of the t-BH group had mostly vanished, while the cells turned spherical and began to abscise. By contrast, the cytoarchitecture of hRPE cells in the dHA group was close to normal. In the fluoxetine co-treatment group, the majority of hRPE cells collapsed as expected.
TEM was applied to examine the intracellular structural changes (Fig. 11). Subsequent to treatment of hRPE cells with 300 µmol/l t-BH for 6 h, the majority of the normal intracellular structure had disappeared, and the following features were observed: chromatin condensated, apoptotic bodies formed, cytoplasm dyeing decreased, mitochondrial edema emerged, and endoplasmic reticulum and Golgi bodies were destroyed. Pretreatment with 10 µmol/l dHA markedly reduced this collapse, although deformation of nuclei and cytoplasmic organoids were still identified. However, co-treatment with 70 µmol/l fluoxetine resulted in severe damage of the intracellular cytoarchitecture.
Taken together, stimulation of TREK-TRAAK K2P channels using dHA exhibited protective effects on hRPE cells under oxidative stress, while blocking the channels using fluoxetine affected the balance of the cytoarchitectural stability. As a result, TREK-TRAAK K2P channels appeared to have a protective role by maintaining the stabilization of the cytoarchitecture in hRPE cells under oxidative stress.

Discussion
The RPE is constituted by a simple layer of cuboidal cells, which is situated under the photoreceptors. It absorbs the majority of light energy and functions as a recycler of photoreceptors disk membrane in the retina, and is the structure that is first injured in multiple retinal degeneration diseases (22). In order to identify a new direction of research and therapies in refractory retinal diseases, such as AMd and RP, the current study explored the protective effects of activated TREK-TRAAK K2P channels on hRPE cells.
In the present study, all members in the TREK-TRAAK K2P channels family were found to be expressed in hRPE cells (Fig. 3). These members were equally expressed with differences in spatial distribution or quantity. Although the specific function of each member of these channels should be further studied in the future, this is not considered to affect the findings of the present study, which focused on the protective effects of all TREK-TRAAK K2P channels on hRPE cells against oxidative stress. Oxidative stress was induced in hRPE cells by exposure to t-BH, which leads to apoptosis accompanied by variations in the levels of apoptosis-associated factors and cytoarchitecture. The channels were activated in the current study, and it was observed that the survival rates of hRPE cells were increased.
Bax, Bcl-2, cleaved-caspase-3 and αB-crystallin are factors associated with cell apoptosis. The caspase-dependent In the flow cytometry plots, the Q4 shows normal hRPE cells, Q3 shows cells at the early apoptosis stage, and Q2 shows cells at the late apoptosis stage. Differences between the control and t-BH groups, the t-BH and DHA groups, and the DHA and fluoxetine co-treatment groups were significant. Data are presented as the mean ± standard error (n=3) of experiments performed in triplicate. ** P<0.01. hRPE, human retinal pigment epithelium; t-BH, tert-butyl hydroperoxide; DHA, docosahexaenoic acid; Q, quadrant.
signaling pathway is one of the cell apoptosis pathways, in which multiple factors are involved. Bax and Bcl-2 are factors that affect the early signaling and regulate the downstream caspase pathway (23). In addition, cleaved-caspase-3 is the major factor that gates the apoptosis process (24), while it is reported that αB-crystallin functions as a trans-acting factor of cleaved-caspase-3 and can inhibit the apoptosis in the treated hRPE cells (25,26). In the present study, the levels of the apoptosis Figure 6. Immunofluorescence analysis revealed the variations in cleaved-caspase-3 and αB-crystallin expression. Following treatment with 300 µmol/l t-BH for 3 and 6 h, the levels of cleaved-caspase-3 were significantly enhanced. DHA was found to restrain this change, whereas fluoxetine was able to neutralize the function of dHA. Variations were slightly different for αB-crystallin: Oxidative stress raised the levels of αB-crystallin after 3-h treatment with 300 µmol/l t-BH, while causing αB-crystallin reduction in the 6-h group. Furthermore, pretreatment with dHA increased the αB-crystallin levels, whereas fluoxetine depressed the expression of αB-crystallin (scale bar, 10 µm). t-BH, tert-butyl hydroperoxide; dHA, docosahexaenoic acid. promoting factors Bax and cleaved-caspase-3 were reduced, while the levels of apoptosis restrainers Bcl-2 and αB-crystallin were enhanced in the t-BH+DHA group (Figs. 4-7). To confirm that the protective effect originated from the TREK-TRAAK K2P channels, an inhibitor of these channels was also applied, namely fluoxetine, which acted efficiently in reversing the protective effects of dHA (Figs. 4-7). However, Bax levels in the cell group co-treated with fluoxetine decreased, and this may be due to the continued oxidative stress destroying the Bax mRNA, which requires further investigation. Notably, the level of αB-crystallin was increased at the early stage of apoptosis, while it decreased at the late stage (Figs. 6 and 7). This may be due to αB-crystallin acting as a natural cellular protective factor in hRPE cells (25,26). In the presence of oxidation, the self-protection system of cells induces the expressions of αB-crystallin; however, if the oxidation lasts for a sufficient amount of time to destroy the self-protection system, then expression level of αB-crystallin may fall. consistent results were also observed for the mRNAs of Bax, Bcl-2, caspase-3 and αB-crystallin (Fig. 8). TREK-TRAAK K2P channels restrained the apoptotic process through decreasing the transcription of Bax and caspase-3 and increasing that of Bcl-2 and αB-crystallin. Taken together, these results indicated that the mechanism of the cellular protective effects of TREK-TRAAK K2P channels are achieved by regulating apoptosis-associated factors.
Variations in the cytoarchitecture are also indicators of apoptosis (27). When the cytoarchitecture collapses, it is difficult to reverse the apoptosis. Therefore, the stabilization of hRPE cells suffering from oxidation was applied to observe the protective effects induced by TREK-TRAAK K2P channels. The cellular dynamics and morphology are linked to different molecular biological factors (including F-actin and α-Tubulin), as well as subcellular structures (28,29). The condition of the nuclei, mitochondria, cytoplasm and cytoplasmic organoids varies along with the status of cellular homeostasis (30). In the current study, it was observed that stimulation of the TREK-TRAAK K2P channels resulted in stabilization of the cytoarchitecture. By contrast, inhibition of these channels was found to reverse this effect.  Following treatment with 300 µmol/l for 3 h, the levels of these mRNAs were tested using RT-PCR. The fold changes in the different groups of human retinal pigment epithelium cells revealed that the oxidative stress enhanced the transcription of Bax and caspase-3, while it reduced that of Bcl-2, and slightly increased that of αB-crystallin. Thus, TREK-TRAAK two-pore domain potassium channels inhibited the oxidation by increasing the transcription of Bax and caspase-3, and decreasing the transcription of Bcl-2, yet further promoting the transcription of αB-crystallin. data are presented as the mean ± standard error (n=3) of triplicate experiments. ** P<0.01 vs. control; ## P<0.01 vs. t-BH or DHA + fluoxetine groups. t-BH, tert-butyl hydroperoxide; dHA, docosahexaenoic acid; RT-PcR, reverse transcription-polymerase chain reaction. Figure 9. Immunofluorescence analysis demonstrating the variations in the cytoarchitecture of human retinal pigment epithelium cells under oxidative stress. After treatment with 300 µmol/l t-BH for 3 h, F-actin and α-Tubulin were initially depolymerized, and partially dissolved (white arrows). In the dHA group, this transformation was not observed, while in the fluoxetine co-treatment group, F-actin and α-Tubulin were further disintegrated. In cells treated with 300 µmol/l t-BH for 6 h, the natural framework of the cytoskeleton was hardly observed. However, dHA pretreatment maintained the majority of the normal structures, although the frameworks were nebulous in parts. By contrast, the cytoskeleton in the fluoxetine group was worse compared with that in the t-BH group (scale bar, 10 µm). t-BH, tert-butyl hydroperoxide; dHA, docosahexaenoic acid.
In conclusion, the present study demonstrated that stimulation of TREK-TRAAK K2P channels enhanced their protective effects on hRPE cells against oxidative stress, which is important in the occurrence and development of retinal degeneration diseases. Thus, these channels may potentially serve as novel targets for future research and therapies for retinal degeneration diseases, such as AMd and RP.