Resveratrol protects against oxidative stress by activating the Keap-1/Nrf2 antioxidant defense system in obese-asthmatic rats

The aim of the present study was to investigate the potential mechanism underlying the anti-obesity-asthmatic effects of resveratrol (RSV) in a rat model of obese-asthma. Rat models of obesity and asthma were established using a high-fat diet and the administration of ovalbumin, respectively. Rats were divided into 7 different groups: A normal control, a normal obese, a normal asthma, a normal obese + asthma, a RSV obese, a RSV asthma and a RSV obese + asthma group. Body weight, Lee index, body fat and lung histopathological changes were evaluated. Serum lipid levels were evaluated using calorimetric methods. Levels of reactive oxygen species (ROS) were examined using enzyme-linked immunosorbent assays. Cellular antioxidant enzyme activities were measured using commercial kits. Levels of kelch-like ECH associated protein 1 (Keap-1) and nuclear factor erythroid 2-related factor 2 (Nrf2) was examined using western blot analysis. The results indicated that obese and asthma rat models were successfully established. It was also demonstrated that RSV decreased fasting blood glucose in obese, asthmatic and obese-asthmatic rats. RSV altered serum lipid levels; it significantly increased high density lipoprotein cholesterol levels and significantly decreased serum triglyceride, serum total cholesterol and very low density lipoprotein levels, compared with untreated obese, asthmatic and obese-asthmatic rats (P<0.05). ROS levels were significantly decreased in the RSV treatment group compared with obese, asthmatic and obese-asthmatic rats (P<0.05). RSV treatment significantly increased catalase, glutathione, glutathione peroxidase and total superoxide dismutase levels compared with untreated obese, asthmatic and obese-asthmatic rats (P<0.05). Furthermore, RSV treatment significantly downregulated Keap-1 and upregulated Nrf2 levels in the heart, lung and kidney tissues of rats compared with untreated controls. Therefore, the results demonstrate that RSV protects against oxidative stress by activating the Keap-1/Nrf2 antioxidant defense system in obese-asthmatic rat models.


Introduction
Obesity is becoming a serious global health problem; rates of obesity have doubled over the past 20-years (1,2). Obesity increases the risk of certain diseases, including cardiovascular disease, diabetes, kidney injury, different types of cancer and various respiratory diseases (3). Epidemiological studies have identified a correlation between obesity and asthma (4,5). Obesity markedly increases the risk of asthma occurring, increases the severity of asthma and decreases lung function (6). The results of animal studies have also indicated an association between obesity and asthma, and have demonstrated that obese and asthmatic animals asthmatic do not fully respond to therapy with the standard drugs used to treat asthma (7,8). Therefore, it is important to develop a drug that inhibits the accumulation of blood fat in order to effectively treat obese patients with asthma.
Previous studies have demonstrated that obesity affects lung tissue; for example, in obesity-associated asthma, obesity induces inflammatory changes, including chronic systemic inflammation and increases levels of leukocytes and pro-inflammatory cytokines (9)(10)(11). Obesity is associated with increased oxidative stress and systemic inflammation; asthma is also associated with the increased expression of malondialdehyde, increased levels of reactive oxygen species (ROS) in membrane phospholipids and increased levels of oxidative stress (12,13). Oxidative stress is characterized by increased ROS levels and the decreased production of antioxidants; these then induce various pathological changes that have critical implications in asthma. Therefore, the present study examined changes in levels of various oxidative biomarkers, including ROS, catalase (CAT), glutathione (14), glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in a rat model of obesity-asthma.
It has been suggested that the kelch-like ECH associated protein 1/nuclear factor erythroid 2-related factor 2 (Keap-1/Nrf-2) antioxidant defense system acts to decrease oxidative stress (15,16). However, to the best of our knowledge, it has not yet been demonstrated whether the Keap-1/Nrf2 antioxidant defense system exhibits antioxidative activity in animals with obese-asthma. Therefore, the present study examined the association between the Keap-1/Nrf2 signaling pathway and oxidative stress-associated molecules in a rat model of obesity-asthma.
Resveratrol (RSV) is a type of non-flavonoid polyphenol that is naturally produced by numerous plants (17). It has been suggested that RSV may prevent or treat a wide range of disorders, including different types of cancer, ischemic diseases and cardiovascular disease (18). It has been demonstrated that the RSV may prevent obesity and help to regulate blood glucose levels in patients with diabetes (19). However, the mechanism by which RSV protects against obesity and regulates blood glucose levels remains unknown. Therefore, the present study aimed to investigate the potential mechanism by which RSV acts to protect against obesity-asthma in a rat model of obese-asthma.

Animals.
A total of 42 adult male Sprague-Dawley weighing 120-130 g were purchased from Beijing Hua Fukang Biotechnology Co., Ltd. (Beijing China). The rats were housed in cages, with 2-3 rats/cage, at a temperature of 25±2˚C and humidity of 50±5%. The rats were kept in a 12 h light/dark cycle and had free access to food and water. The rats in different groups were housed in separate cages. The present study was performed following the guidelines of the Care and Use of Laboratory Animals of National Institute of Health (20) and was approved by the Ethics Committee of Dalian Medical University (Dalian, China).
Establishment of the obese-asthmatic model and rat grouping. The 42 rats were randomly divided into the following 7 groups (all n=6): A normal control group, a normal asthma group, a normal obese group, an obese + asthmatic group, a RSV asthma group, a RSV obese group and a RSV obese + asthma group.
The normal control group was fed a normal diet for 6 weeks. The normal obese group was fed with high-fat diet for 6 weeks. Rats in the normal asthma group received an intraperitoneal injection of ovalbumin (OVA) suspension (0.5 ml/rat), formed by dissolving 1 mg OVA (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and 20 µg calmogastrin (Sigma-Aldrich; Merck KGaA) into 0.5 ml 0.9% normal saline, on the first and eighth day following 6 weeks of a normal diet. On the fifth day, 10 g/l OVA suspension (10 ml) was administrated to the normal asthma group rats using the atomization excitation method for 30 min, for three times each week. Rats in the asthma groups received OVA over a 2-week period. Rats in the obese + asthma group were fed a high-fat diet for 6 weeks and received OVA in the same manner as the normal asthma group, following a previously described protocol (21). Rats in the RSV asthma, RSV obese and RSV obese + asthma groups were fed either a normal or a high-fat diet for 6 weeks. Rats in the RSV and RSV obese + asthma groups received OVA in the same manner as the normal asthma and obese + asthma groups. All rats in these groups received a supplement of 0.03% RSV (Nanjing Oddfoni Biol. Tech. Co. Ltd., Nanjing, China) in their diet (21).
Body weight evaluation. Rats were weighed every day. The rats were weighed prior to anesthetization. All rats were anesthetized using ketamine (50 mg/kg) and xylazine (10 mg/kg). Changes in body weight (%), the Lee index (mg/mm) and body fat percentage (%) were evaluated and calculated following previously published protocols (22,23).
Lung histopathological evaluation in obese-asthma rat model. The rats were sacrificed by 100 mg/kg body weight intraperitoneal injection of thiopental (Shanghai New Asiatic Pharmaceutical Co. Ltd., China) and the lung, heart and kidneys were immediately removed for histopathological evaluation. Sacrifice was confirmed when rats stopped breathing and their hearts ceased beating. The tissues were fixed using 4% formaldehyde (Beyotime Institute of Biotechnology, Haimen, China) in PBS for 15 min at room temperature. The endogenous peroxidase was inactivated by 3% hydrogen peroxide for 5 min at room temperature. The tissues were cut into of 4-µm-thick sections. The sections were stained with haematoxylin-eosin at room temperature for 15 min. Lung tissues were evaluated using a light microscope. Pathological changes in the lung tissues, including airway smooth muscle hyperplasia, emphysema and edematous changes, were recorded and calculated according following a previously published protocol (21). Pathological grades were defined using the following system (21): Grade 0, no pathological changes; grade 1, patchy pathological changes; grade 2, local pathological changes; grade 3, scatter pathological changes and grade 4, severe pathological changes in most parts of the lung tissue.
Blood glucose and serum lipid evaluation. All rats were fasted overnight for ≥8 h and 2 ml blood was taken from the tail vein in the morning. Blood glucose was measured using an On-Call glucose analyzer (ACON Biotech Hangzhou Co., Ltd., Hangzhou, China). Levels of fasting serum lipids, including high density lipoprotein cholesterol (HDL-C; cat. no. A112-1), serum triglyceride (TG; cat. no. A110-1), serum total cholesterol (TC; cat. no. A111-1) and very low density lipoprotein (vLDL; cat. no. H249) levels were evaluated using ELISA with the HDL-C, TG, TC, vLDL test kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively. The fasting serum lipids were examined using an ELISA reader (ELX800; BioTek Instruments, Inc., Winooski, VT, USA). Levels of serum lipids were calculated using the Friedewald Equation (24).

ROS determination.
Rat tissues (heart, lung and kidney) were washed with PBS in ice to remove blood and necrotic tissue. Fibrosis, fat and blood vessels were also removed. Subsequently, tissues were sliced into 1-mm 3 sections and these slices were ground to obtain the cell suspension. Cell suspensions were centrifuged at a speed of 500 x g for 10 min at room temperature and supernatants were then removed. ROS levels in the heart, tissue and lungs of rats from each group were measured using an enzyme-linked immunosorbent assay kit (cat. no. 88-5930; Beijing Leagene Biotech Co., Ltd., Beijing, China) following the manufacturer's protocol. Obtained cells were resuspended in PBS and the cell density was adjusted to 10 6 /sample. Cells were then incubated with 10 µM dichloro-dihydro-fluorescein diacetate at 37˚C for 45 min, centrifuged at 1,000 x g for 10 min at 37˚C and cells were then collected. Cell fluorescence was measured at 500/525 nm using a microplate reader (Synergy™ H4; BioTek Instruments, Inc.).

Evaluation of cellular antioxidant enzyme activities.
The activities of cellular antioxidant enzymes were evaluated using a CAT assay kit (cat. no. TO1070-100T), a GSH assay kit (cat. no. TO1031-100T) (both Beijing Leagene Biotech Co., Ltd.), a T-SOD assay kit (cat. no. A001-1-1) and a GSH-PX assay kit (cat. no. A005) (both Nanjing Jiancheng Bioengineering Institute) following the manufacturer's protocol. Tissues were treated using the aforementioned processes used to determine ROS levels; supernatants were removed and pellets were collected post-centrifugation. Pellets were then lysed using 0.5% trypsin at 37˚C for 20 min. Lysed cells were then centrifuged at 10,000 x g at room temperature for 10 min and supernatants were then collected. CAT activity was evaluated at 562 nm and calculated according to the H 2 O 2 decomposition rate at 405 nm. GSH activity was evaluated at 420 nm and calculated using the GSH calibration curve. T-SOD activity was evaluated at 560 nm and was determined according to its ability to suppress the oxidation of hydroxylamine by the superoxide anion from the xanthine oxidase system. GSH-Px activity was evaluated at 412 nm according to the oxidation rate of decreased glutathione to the oxidized glutathione by H 2 O 2 under GSH-Px catalysis using a microplate reader.
Statistical analysis. All data were assessed using SPSS software 20.0 (IBM Corp, Armonk, NY, USA) and presented as the mean ± standard deviation. All data were obtained from at least six independent experiments or tests. A Student's t test was used to determine whether differences between two groups were significant. One-way analysis of variance followed by Tukey's post hoc test was used to compare measurement data among multiple groups. P<0.05 was determined to indicate a statistically significant difference.

Results
Successful establishment of obese-asthma rat models. To confirm the establishment of the obese, asthma and obese-asthma rat models, changes in body weight, the Lee index and body fat percentage were assessed to determine the establishment of the obese models. Airway smooth muscle hyperplasia, emphysema and edematous changes, were assessed to determine the establishment of the asthmatic models. The results demonstrated that all obese indices, including body weight (Fig. 1A), Lee index values (Fig. 1B) and body fat percentage (Fig. 1C) were significantly increased in the obese-asthma and normal obese groups compared with the normal control group (all P<0.05). Furthermore, all asthma associated indices, including emphysema (Fig. 1D), airway smooth muscle hyperplasia (Fig. 1E) and edematous changes (Fig. 1F), were all significantly increased in the normal-and obese-asthma normal groups compared with the normal control group (all P<0.05).

RSV decreases fasting blood glucose levels.
To evaluate the effects of RSV on blood glucose levels in obese-asthmatic rats, fasting blood glucose levels were examined. The results indicated that fasting blood glucose levels in the RSV asthma group were significantly decreased compared with the normal asthma group (P<0.05; Fig. 2A) and that fasting blood glucose levels in the RSV obese group were also significantly decreased compared with the normal obese group (P<0.05). Furthermore, the fasting blood glucose levels were significantly reduced in the RSV obese + asthma group compared with the normal obese + asthma group (P<0.05; Fig. 2A). There were no significant differences in fasting blood glucose levels between the normal obese and asthma groups ( Fig. 2A).

RSV improves serum lipid levels.
To investigate the effects of RSV on serum lipids, HDL-C, TG, TC and vLDL levels were evaluated in the present study. The results demonstrated that HDL-C levels in the RSV asthma group were significantly increased compared with the normal asthma group (P<0.05; Fig. 2B) and were also significantly increased in the RSV obese group compared with the normal obese group (P<0.05). Furthermore, HDL-C levels were significantly increased in the RSV obese + asthma group compared with the normal obese + asthma group (P<0.05; Fig. 2B). By contrast, TG (Fig. 2C), TC (Fig. 2D) and vLDL (Fig. 2E) levels were significantly decreased in the RSV asthma group compared with the normal asthma group, in the RSV obese group compared with the normal obese group and in the RSV obese + asthma group compared with the normal obese + asthma group (all P<0.05). There were no significant differences in HDL-C, TG, TC and vLDL levels between the normal obese and normal asthma groups (Fig. 2).
RSV inhibits ROS production. ROS levels may reflect oxidative status; therefore, ROS levels were measured in the heart, lung and kidney tissues of obese-asthmatic rats. The results indicated that ROS levels in the heart tissue were significantly decreased in the RSV asthma, RSV obese and RSV obese + asthma groups compared with the normal asthma, normal obese and normal obese + asthma group, respectively (all P<0.05; Fig. 3A). ROS levels in the lung (Fig. 3B) and kidney tissue (Fig. 3C) were also significantly decreased in each of the groups that received RSV treatment, compared with their respective controls (P<0.05). Furthermore, there were no significant differences in ROS levels in the lung, kidney and heart tissues between the normal obese and asthma groups (Fig. 3).

RSV enhances cellular antioxidant enzyme activities.
In the present study, levels of antioxidant enzymes including CAT, GSH, GSH-Px and T-SOD, in the heart, lung and kidney tissue of rats were analyzed using commercial kits. The results All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; RSV, resveratrol; HDL-C, high density lipoprotein cholesterol; TG, serum triglyceride; TC, serum total cholesterol; vLDL, very low density lipoprotein. demonstrated that RSV treatment significantly increased CAT levels in all groups compared with their respective controls (all P<0.05). This was the case in the heart (Fig. 4A), lung (Fig. 4B) and kidney tissues (Fig. 4C). There were no significant differences in CAT levels between the normal obese and asthma groups in any of the tissues assessed (Fig. 4). GSH (Fig. 5), GSH-Px (Fig. 6) and T-SOD levels (Fig. 7) were all significantly increased in each of the RSV treatment groups (the RSV asthma, RSV obese and RSV obese + asthma groups) compared with their respective controls (the normal asthma, normal obese and normal obese + asthma groups, respectively) in the heart, lung and kidney tissues (all P<0.05). There were no significant differences in GSH (Fig. 5), GSH-Px (Fig. 6) and T-SOD levels (Fig. 7) in the lung, kidney and heart tissues between the normal obese and asthma groups.

RSV downregulates Keap-1 expression.
In the present study, the Keap-1/Nrf-2 antioxidant defense system molecule, Keap-1, was assessed via western blotting. The results indicated that Keap-1 expression in the RSV asthma group was significantly decreased compared with the normal asthma group in heart tissue (P<0.05; Fig. 8A). Keap-1 expression was also significantly decreased in the RSV obese group compared with the normal obese group (P<0.05; Fig. 8A). Furthermore, Keap-1 expression was significantly decreased in the RSV obese + asthma group compared with the normal obese + asthma group (P<0.05; Fig. 8A).
The expression of Keap-1 in the lung and kidney tissues was also examined. The results demonstrated that RSV treatment significantly decreased Keap-1 expression in the lung (Fig. 8B) and kidney tissue ( Fig. 8C; all P<0.05).
There were no significant differences in Keap-1 expression in any of the tissues between the normal obese and asthma groups in any of the tissues assessed (Fig. 8).
RSV upregulates Nrf2 expression. Another Keap-1/Nrf2 antioxidant defense system molecule, Nrf2, was also examined in the present study. The results demonstrated that Nrf2 expression in the RSV asthma and RSV obese groups was significantly increased compared with the normal asthma and normal obese groups, respectively, in the heart tissue (P<0.05; Fig. 8A). Furthermore, Nrf2 expression was significantly increased in the RSV obese + asthma group compared with the normal obese + asthma group (P<0.05; Fig. 8A).
Nrf2 expression in the lung and kidney tissues was also examined. The results indicated that the RSV treatment significantly increased Nrf2 expression in the lung (Fig. 8B) and kidney tissue (Fig. 8C) in all groups that received RSV treatment, compared with their respective controls (all P<0.05).
There were no significant differences in Nrf-2 expression in the lung, kidney and heart tissue between the normal obese and asthma groups (Fig. 8).

Discussion
Obesity is considered to be a chronic and low-grade pro-inflammatory disease; however, it remains unknown whether this inflammation induces the development of asthma and the inflammation of airways in humans or animals (23,25). Therefore, the current study administered a high-fat diet to rats in order to establish a model of obesity, used OVA to establish a model of asthma and assessed whether there is an association between ROS/antioxidant levels and obese-asthma.
The results of the present study indicated that rats with diet-induced obesity exhibited weight gain, increased Lee index values and fat percentages, which was consistent with the results of a previous study (26). Furthermore, rats with OVA-induced asthma exhibited airway smooth muscle hyperplasia, emphysema and edematous changes, which were also consistent with the results of a previous study (27). These changes in obesity and asthma indices suggest that the obese, asthma and obese-asthmatic models were all established successfully, and therefore could be used in subsequent experiments. All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; ROS, reactive oxygen species; RSV, resveratrol; OD, optical density.
Previous studies have investigated the effects of RSV in the treatment of various diseases, including Alzheimer's disease (28), heart disease (29) and Parkinson's disease (30). Studies have also investigated the potential of RSV to treat obesity-associated diseases, including non-alcoholic fatty liver disease (31) and the asthmatic disorders, such as allergic asthma (32). However, the specific mechanisms by which RSV induces therapeutic effects in such disorders remains unknown. Therefore, the current study investigated the anti-obese and anti-asthmatic effects of RSV and its associated mechanisms of action.
The results of the current study indicated that the products of diet-induced obesity, including HDL-C, TG, TC and vLDL, were significantly increased in obese and asthmatic rats compared with normal control rats. However, RSV significantly reversed the increase in HDL-C, TG, TC and vLDL that occurred in obese and asthmatic rats. These effects of RSV are consistent with the results observed in Figure 5. Levels of the cellular antioxidant enzyme GSH in different tissues. GSH levels in the (A) heart, (B) lung and (C) kidney tissue. All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; RSV, resveratrol; GSH, glutathione. All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; CAT, catalase; RSV, resveratrol.
a previous study (33), which indicated that RSV improves lipid metabolism in patients with liver disease. Xie et al (34) also reported that RSV regulates lipid metabolism in hyperlipidemic mice. The effects of RSV on lipid metabolism suggest that it may be used to treat patients with hyperlipoidemia-associated disorders. However, obesity-associated parameters, including fasting blood glucose, HDL-C, TG, TC and vLDL levels in asthmatic rats were also enhanced compared with normal rats. These results regarding lipid-associated parameters in asthma are similar. The results of a previous study investigating patients with severe acute asthma indicated that they exhibited increased All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; RSV, resveratrol; T-SOD, total superoxide dismutase. Figure 6. Levels of the cellular antioxidant enzyme GSH-Px in different tissues. GSH-Px levels in the (A) heart, (B) lung and (C) kidney tissue. All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; RSV, resveratol; GSH-Px, glutathione peroxidase. hyperglycemia and hyperlipemia (35). In the current study, a rat model of acute asthma was established by intraperitoneal administration of OVA suspension. It may be that the increased levels of HDL-C, TG, TC and vLDL observed in rats with asthma observed in the current study were caused by the acute administration of OVA suspension. Further studies are required to explain these changes in HDL-C, TG, TC and vLDL levels that occurred in the asthmatic rats included in the present study.
Oxidative stress has two main effects on intercellular physiological processes; it enhances ROS production and decreases levels of antioxidants (36). Increased oxidative stress in airways is considered to be a mechanism by which obesity enhances the severity of asthma (13,37). Ochs-Balcom et al (38) also demonstrated that obese-asthmatic patients with obstructed airways experienced more severe asthma and severe oxidative stress. It was hypothesized that the imbalance between antioxidants and ROS may serve a critical role in the pathological process of obese-asthma. Therefore, the current study evaluated ROS and antioxidant (CAT, GSH, GSH-Px and T-SOD) levels in the heart, lung and kidney tissues of obese and/or asthmatic rats undergoing RSV treatment. The results indicated that RSV treatment significantly decreased levels of ROS in the heart, lung and kidney tissues of obese and asthmatic rats. Furthermore, RSV significantly increased CAT, GSH, GSH-Px and T-SOD levels in the heart, lung and kidney tissues of obese and asthmatic rats. The antioxidative effects of RSV determined in the current study were consistent with the results of a study by Guo et al (39), which indicated that RSV inhibits high glucose-triggered oxidative stress by suppressing the production of ROS. Chen et al (40) also reported that RSV protects hepatic cells against oxidative stress by enhancing SOD activity. Although Sood et al (41) indicated that oxidative stress in airways may act as a signaling pathway between obesity and asthma, the present study is the first to indicate the role of RSV in protecting against oxidative stress by decreasing ROS levels and increasing the expression of CAT, GSH-Px, GSH and T-SOD in obese-asthmatic rat models.
It has been demonstrated that the Keap-1/Nrf2 antioxidant defense system is an important cyto-protective mechanism against the oxidative stress associated with increased ROS and decreased antioxidant levels (42). Therefore, it was speculated that the Keap-1/Nrf2 antioxidant system may participate in the antioxidative stress processes of RSV in obese-asthmatic rat models. Key molecules of the Keap-1/Nrf2 signaling pathway, including Keap-1 and Nrf2, were assessed in the current study. The results illustrated that RSV significantly downregulated Keap-1 and upregulated Nrf2 levels in obese, asthmatic and obese-asthmatic rats. Wang et al (43) reported that the mitogen-activated protein kinase/Nrf2 signaling pathway is stimulated in rats with high-fat diet induced obesity. Previous studies also explored the role of Nrf2 and Keap-1 in the development of allergic, bronchial and childhood asthma (44,45). However, few studies have investigated the association between the All data are presented as the mean ± standard deviation and were obtained from at least six independent experiments or tests. 1, Normal control group; 2, Normal obese group; 3, Normal asthma group; 4, Obese + asthma group; 5, RSV obese group; 6, RSV asthma group; 7, RSV obese + asthma group; RSV, resveratrol; Keap-1, kelch-like ECH associated protein 1; Nrf2, nuclear factor erythroid 2-related factor 2. pathogenesis of obesity or asthma and the Keap-1/Nrf2 antioxidant defense system; to the best of our knowledge, the present study was the first to do so. Furthermore, the current study also indicated that RSV triggered the downregulation of Keap-1 and increased the expression of Nrf2 in the obese-asthmatic rats, which is consistent with the results of a previous study (46). It has also been demonstrated that RSV attenuates obesity-associated inflammation by activating the AMP-activated protein kinase and its signaling pathway (47). However, the present study indicated that RSV attenuates obesity-asthmatic associated oxidative stress via the Keap-1/Nrf2 antioxidant defense system. This suggests that RSV prevents high-fat-induced obese and OVA-induced asthma by activating the Keap-1/Nrf2 antioxidant defense system and may therefore be a promising therapeutic target for treating obesity-asthma.
The current study had a few limitations. Firstly, dynamic changes in Keap-1/Nrf2 expression were not evaluated. Future studies should evaluate the expression of Nrf2 in the nuclear fraction and Keap-1 in the cytosolic fraction, following a previously described protocol (48). Secondly, Nrf-2 and Keap-1 expression are not always associated with Nrf-2 activation (46); therefore, to ascertain whether Nrf-2 is activated, the expression of Nrf-2 nuclear translocation and/or the Nrf-2 target gene should be determined. Thirdly, the association between asthma and obesity was not investigated in the current study; therefore, it could not be clarified whether asthma is caused by obesity. Finally, it was not confirmed in the current study whether RSV regulates the Keap-1/Nrf-2 antioxidant defense system.
In conclusion, the results of the present study demonstrated that RSV treatment decreases serum lipid levels, decreases ROS production and increases antioxidant enzyme activities in a rat model of obese-asthma. Furthermore, RSV treatment decreased Keap-1 and increased Nrf2 levels. Therefore, the results of the current study indicate that RSV protects against obese-asthma associated oxidative stress by activating the Keap-1/Nrf2 antioxidant defense system in obese-asthmatic rats.