The interplay of BMP4 and IL-7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia/reperfusion

The number and function of intestinal intraepithelial lymphocytes (IELs) have been found to be significantly reduced following induction of acute intestinal mucosal damage via intestinal ischemia/reperfusion (I/R). However, the mechanism underlying this reduction remains unclear. Therefore, it was hypothesized that the interplay of bone morphogenetic protein (BMP)4 and interleukin (IL)-7 regulates IEL function and number. Recent studies have demonstrated that the different components of the BMP2/4 signaling pathway are expressed in intestinal epithelial cells (IECs) via the activation of nuclear factor (NF)-κB. In the present study, reverse transcription-polymerase chain reaction analysis and flow cytometry demonstrated that IELs express BMP receptors (BMPRIA, BMPRIB, ActRIA and BMPRII) and non-canonical signal transduction molecules (NF-κB). An in vivo mouse intestinal I/R model was used, and I/R was shown to increase the expression of BMP4 in IECs and upregulate the expression levels of BMPRIA, BMPRIB and phosphorylated NF-κB in IELs. Following isolation and culture of IELs, it was observed that exogenous BMP4 also upregulated the expression of BMPRIA and BMPRIB and activated NF-κB signaling in IELs, inducing IEL apoptosis. In addition, the rate of apoptosis of IELs decreased following treatment with the BMP-specific antagonist NOGGIN or with the NF-κB inhibitor pyrrolidine dithiocarbamate. Furthermore, it was observed that exogenous IL-7 can decrease BMP4 protein expression in IECs and the expression of phosphorylated NF-κB protein in IELs. The findings of the present study suggest that, under conditions of I/R, IEC-derived BMP4 activates NF-κB signaling in IELs, inducing IEL apoptosis, further aggravating the dysfunction of the intestinal mucosal barrier. However, these effects may be alleviated by IL-7 treatment. Therefore, BMP4 and IL-7 appear to be involved in the interaction between IECs and IELs and in the mechanism underlying intestinal mucosal barrier dysfunction.


Introduction
Intestinal ischemia̸reperfusion (I̸R) injury is a common clinical event that occurs during surgery for abdominal aortic aneurysm, mesenteric artery occlusion, small bowel transplantation, cardiopulmonary bypass, strangulated hernias, trauma, and shock (1). acute intestinal ischemia is a life-threatening emergency that is associated with substantial morbidity and mortality (2). However, the exact mechanism involved in the pathogenesis of acute intestinal ischemia has not yet been clearly defined. The intestinal mucosal barrier is a complex entity comprising different structural, mechanical, physical, immune, biological and chemical components, among others. due to the presence of intestinal epithelial cells (iecs) and intestinal intraepithelial lymphocytes (iels) in the intestinal mucosa, the epithelial layer is a key factor that determines the barrier function of the intestinal mucosa (3,4). IELs mainly consist of t cells, which are interspersed among the iecs in the small intestine; there are ~20 iels for every 100 iecs in the small intestine (5). IELs play an important role in the maintenance of mucosal homeostasis, as they form the first line of immune defense against invasion, while they also preserve the integrity of the mucosal barrier (6). I̸R-induced acute intestinal mucosal damage significantly promotes apoptosis of IELs and affects the phenotype and function of IELs (7). The immune function of IELs may be regulated by IECs. In order for the intestinal barrier to function properly, there must be communication between the iels and the iecs, which is initiated through the activation of iecs by direct contact or through the effects of secreted factors on iels, maintaining their growth and metabolism. However, the mechanism underlying this dialogue remains unclear (8).
Bone morphogenetic proteins (BmPs) play a pivotal role in the patterning and cellular fate of numerous tissues and organs, including the intestinal mucosal epithelium (9). over 30 BmPs have been reported, and they all belong to the larger transforming growth factor (tgf)-β family. BMP signaling includes the canonical Smad signaling pathway and the non-canonical mitogen-activated protein kinase and nuclear factor (nf)-κB signaling pathways (10). BMPs are primarily found in epithelial cells (ECs) in the intestinal mucosal barrier. it was previously reported that the abundance of BmP2, BmP4 and BmP receptors at the protein level was greater in the total parenteral nutrition (tPn) group compared with that in the control (11). In addition, hypoxia and I̸R were found to increase the expression of BMP2̸4 and upregulated the expression of the BmP receptors BmPria and BmPrii in an in vivo mouse intestinal I̸R model. I̸R also activated the NF-κB signaling pathway, which led to increased levels of inflammatory factors, such as tumor necrosis factor (tnf)-α and interleukin (il)-6; this in turn aggravated the damage to the intestinal mucosal barrier (12). Therefore, the expression of BMP4 was increased in the intestinal mucosal epithelial cells under simulated stress conditions. Moreover, BMP2̸4 signaling is involved in thymocyte development (13), and it has been reported that it directly regulates fetal t-cell development through BMPRIA. Additionally, use of the specific BMP antagonist Noggin revealed that BMP2̸4 signaling is a negative regulator of fetal t-cell development (14). BMP4 treatment of human-mouse fetal thymus organ culture resulted in a reduction in the recovery of human cells and the inhibition of cd34 + progenitor cell proliferation and differentiation (15). NF-κB signaling proteins comprise a family of pleiotropic transcription factors that are involved in the embryonic development of various organs, inflammation, the immune response, cell survival, proliferation and differentiation (16). Additionally, it was determined that nf-κB is activated within a few hours after I̸R, leading to upregulation of inflammatory proteins (17). il-7 is produced by stromal cell components in the bone marrow, thymus and in peripheral lymphoid compartments, and is a master regulator of t-cell development and homeostasis (18,19). IL-7 binds to the IL-7R heterodimer, which is composed of the IL-7-specific IL-7R α-chain (cd127) and the γc-chain (CD132). IL-7 induces CD127, which is linked to the activation of the janus kinase̸signal transducer and activator of transcription 5 (STAT5) signaling pathway. This pathway is crucial for cell survival and the upregulation of anti-apoptotic Bcl-2 proteins, which maintain t-cell homeostasis and prevent the progression of inflammation (20,21). IL-7 signaling and the cd127 receptor were found to maintain the balance of regulatory t cells in vivo (22). In the intestine, IL-7 is produced by iecs and, in turn, il-7 receptors (cd127 and cd132) have been detected on IELs (23). The IL-7̸IL-7R signaling pathway plays a key role in the regulation of iels (24), as il-7 has been demonstrated to increase the numbers and functional capacity of IELs. it was previously demonstrated that tPn results in loss of iec-derived il-7, which leads to a marked weakening in the function and phenotype of IELs (25,26). IL-7 produced in the thymus is also essential for the development of IELs (27). therefore, IL-7̸IL-7R signaling appears to be crucial for the maintenance of the function and growth of IELs.
it was reported that the interplay of BmP4 and il-7 is key to the maintenance of the human thymic prog enitor population. BMP4 downregulates the expression of CD127 in the precursor cell population, which leads to a reduction in the proliferation and differentiation of cd34 + progenitor cells. In addition, IL-7 stimulates the expansion and differentiation of intrathymic precursors (13,15). Therefore, it was hypothesized that the interplay between BmP4 and il-7 regulates the number and function of iels following I̸R. our data indicated that iels express functional BMP receptors. BMP proteins activate the nf-κB signaling pathway, which increases apoptosis, but they also inhibit apoptosis induced by IL-7̸IL-7R signaling and maintain the number of iels, indicating that the interplay between the BmP4 and il-7 signaling pathways is important in the dialogue between IECs and IELs.
Western blot analysis. the cells were washed twice with phosphate-buffered saline (PBS) prior to lysis in cold riPa buffer [50 mm tris, 150 mm nacl, 1% triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SdS) and 2 mm sodium pyrophosphate]. The samples were then mixed with loading buffer and boiled for 5 min prior to electrophoresis. the proteins were loaded onto 8-10% SdS-Page gels and were subjected to electrophoresis at 100 V for 2 h. After electrophoresis, the proteins were electroblotted onto nitrocellulose membranes at 200 mA for 2 h. Non-specific binding was blocked by incubation in tris-buffered saline (tBS) with 0.1% Tween-20 (TBS-T) and 5% skimmed milk. The transferred membranes were incubated overnight at 4˚c with the following primary antibodies: anti-BmP4 (1:500), anti-BMPRIA (1:200) and anti-gAPDH (1:5,000). After washing three times in tBS-t, the membranes were incubated with anti-rabbit igg (cat. no. Ba1001; Zhongshan Bio-Tech Co., Ltd., Beijing, China) conjugated to horseradish peroxidase at a dilution of 1:3,000 in tBS-t containing 5% skimmed milk for 1 h at 37˚C. After three additional washes in TBS-T, the signals were visualized with the SuperSignal West Pico Trial kit (Pierce Biotechnology, Inc., Rockford, IL, uSA) and detected with image Station 4000r (Kodak, rochester, ny, uSA).
Animal experiments. Male C57BL̸6 pathogen-free mice, aged 6-8 weeks (weight, 20-30 g), were purchased from the laboratory animal center of the third military medical university (chongqing, china), and maintained in a temperature-, humidity-and light-controlled environment. The mice were randomly assigned to one of two groups (n=12/group), the first of which was the I̸R group. following intraperitoneal anesthesia with 40 mg̸kg pentobarbital, the abdomen was opened at the midline, and the superior mesenteric artery was occluded for 30 min using non-traumatic vascular clamps, followed by predefined times of reperfusion. The second group was the sham group, which included animals that were subjected to anesthesia and laparotomy without ischemia. all mice were sacrificed after 6 h by co 2 asphyxiation. the jejunal part of the small intestine was cut along the longitudinal axis, washed in physiological saline, and immediately frozen in liquid nitrogen and stored at -70˚c until use. The present study was approved by the ethics committee of Xinqiao Hospital, the Third Military Medical university. all animals were handled according to the guidelines for the care and use of laboratory animals (NIH publication no. 85-23, revised 1996).
Immunofluorescence analysis. in the present study, 10-µm frozen sections were cut from the jejunum and were fixed onto slides. After fixation in 4% formaldehyde for 20 min, the sections were incubated in 3% H 2 o 2 for 30 min to quench endogenous peroxidase activity. Non-specific binding was blocked with 5% bovine serum albumin (BSa) in PBS for 30 min at room temperature. the sections were then incubated overnight at 4˚c in 3% BSa in PBS with the following primary antibodies: anti-BmP4 and anti-il-7, at a dilution of 1:50. The sections were washed three times with BSa in PBS and were incubated with Alexa 488-conjugated goat anti-rabbit antibody for 1 h at RT. After the nuclei were stained with DAPI, images were captured and analyzed with a leica tcSSP confocal imaging system (Leica, Heidelberg, germany).
Isolation of IELs from the small intestine. the isolation of iels was performed according to the protocol of mosley and Klein (28). Briefly, the small bowel was placed in RPMI-1640 tissue culture media supplemented with 10% fBS (both from gibco; Thermo Fisher Scientific). The sections were cut into 5-mm pieces, washed in an extraction buffer, and incubated in the same buffer with continuous brisk stirring at 37˚c for 25 min. The supernatant was filtered rapidly through a glass wool column. After centrifugation, the pellets were purified in 40% isotonic Percoll (gE Healthcare Biosciences, Pittsburgh, PA, uSA) and reconstituted in RPMI tissue culture media. the purity of the iels was >95%, as assessed by trypan blue exclusion staining. Isolated IELs were counted with a neubauer hemocytometer (reichert technologies, Buffalo, NY, uSA).
IEL cell culture. iels were isolated from the small intestine and grown in rPmi-1640 supplemented with 10% fBS, 100 Iu̸ml penicillin and 100 mg̸ml streptomycin. The IELs were cultured at 37˚c under normoxic (5% Co 2 and 20% o 2 ) conditions (Thermo Fisher Scientific, Inc.), and the viability of the iels automatically decreased under culture without interference. For flow cytometry, BMP4, NoggIN, PDTC and IL-7 were added to the medium for 6 h.
Flow cytometric analysis. the iel phenotype was assessed with fluorescent antibody staining detected by flow cytometry. The following anti-mouse monoclonal antibodies were used: cd45-aPc, BmPria-fitc, BmPriB-fitc and CD127-Alexa Fluor 488. Isotype-matched, irrelevant antibodies were used as negative controls. The apoptotic ratios for the IELs in the different groups were measured by flow cytometry according to the manufacturer's instructions. Double staining for FITC-Annexin V binding and cellular dna using propidium iodide were performed as described in the manufacturer's protocol. The acquisition and analysis were performed with moflo (Beckman coulter, Brea, ca, uSA).
Statistical analysis. Statistical analyses were performed with SPSS 13.0 statistical software (SPSS Inc., Chicago, IL, uSA). All data are expressed as the means ± standard deviation. The results were analyzed using analysis of variance. Statistical significance was defined as P<0.05.

Results
The BMP signaling pathway is functionally active in IELs. it has been previously reported that iec dysfunction induced by BMP2̸4 activates NF-κB signaling following I̸R (12). in a similar manner, the inhibition of cd34 + progenitor cell differentiation under stimulation of the BMP2̸4 signaling pathway has also been demonstrated (15). To determine whether BMP4 signaling also activates nf-κB signaling in iels, it was first demonstrated by flow cytometry that the purity of IELs isolated from the small intestine of mice reached 94.5%, as shown in Fig. 1A. RT-PCR analysis demonstrated that IELs expressed three type i BmP receptors (BmPria, BamPriB and actria), as well as one type II BMP receptor (BMPRII). Similarly, IELs expressed specific RNA for p-NF-κB (iKKβ), as shown in Fig. 1B. The flow cytometric analysis revealed that the iels expressed BMPRIA (4.24±0.37%), BMPRIB (12.8±0.67%), and phosphorylated nf-κB (P65) (7.14±1.36%), as shown in Fig. 1C. therefore, it may be hypothesized that BmP binds to BmP receptors and activates nf-κB signaling in IELs of the small intestine.
I̸R induces an increase the expression of BMP4 in IECs and upregulates BMP receptors and the phosphorylation of NF-κB in IELs. a previous study revealed more severe destruction of the intestinal morphology and some changes in protein secretion 6 h after I̸R (29); moreover, the expression of the BMP4 protein was determined in IECs. the protein level of BmP4 in iecs was analyzed by immunofluorescence in the I̸R group compared with the sham group, and the expression level of BMP4 was found to be markedly increased in IECs, as shown in Fig. 2A. Moreover, it was recently repo rted that I̸R significantly altered iel-derived cytokine expression in IELs (7). To better understand the changes in BmP receptors and nf-κB signaling in iels following I̸R, flow cytometry was performed and it demonstrated that the expression of BMPRIA, BMPRIB and phosphorylated nf-κB increased following I̸R (BMPRIA, 12.66±0.91%; BMPRIB, 16.70±2.22%; and p-nf-κB, 8.56±2.87%), as shown in Fig. 2B. These data suggest that I̸R induces expression of iec-derived BmP4 and activation of nf-κB signaling in IELs.
Exogenous BMP4 regulates the NF-κB signaling pathway in IELs. the primary BmP receptors include two type i receptors (BmPria and BmPriB) and one type II receptor (BMPRII). typically, BmP4 binds to BmPria and BmPriB, both of which have a high-affinity binding site for BMP4 (9). To determine whether the activation of nf-κB signaling in iels involves iec-derived BmP4, iels from the small intestines of mice were isolated and cultured. it was observed that the protein levels of BmPria, BmPriB and phosphorylated nf-κB were highly upregulated in iels that were cultured in the presence of BmP4 (30 ng̸ml), as assessed by flow cyto metry (BMPRIA, 9.88±0.56%; BMPRIB, 15.00±1.42%; and p-nf-κB, 11.33±1.45%). However, this effect was abolished by the BmP-specific antagonist NoggIN (30 ng̸ml), as shown in Fig. 3. Therefore, it is hypothesized that I̸R induces the activation of NF-κB signaling in iels, possibly through IEC-derived BMP4.
BMP4̸NF-κB signaling enhances the apoptosis of IELs cells. the effects of BmP4 on apoptosis of iels were next evaluated following addition of exogenous recombinant BMP4 to the IEL cultures. Although the viability of the IELs decreased from 94.5 to 45% under without interference cultured, it was also observed that BmP4 induces iels to undergo apoptosis (13.20±2.25%). Activation of NF-κB in iels by iec-derived BMP4 (30 ng̸ml) was observed. The blockade of BMP activity by the BmP antagonist noggin (30 ng̸ml), or by PDTC (30 ng̸ml), resulted in a marked decrease in IEL apoptosis, as shown in Fig. 4. Therefore, these results strongly suggest that iec-derived BmP4 activates nf-κB signaling in iels, which leads to enhanced IEL apoptosis.

I̸R induces a decrease the expression of IL-7 in IECs and downregulates the expression of IL-7Rα̸CD127 and phosphorylated STAT5 in IELs.
iec-derived il-7 plays a key role in the regulation of the development and homeostasis of neighboring IELs. IL-7 was also shown to significantly affect the phenotype and function of iels, and caused an increase in the number of IELs (30). In a previous study, an decrease in IL-7 expression was confirmed in an intestinal I̸R mouse model (31). In addition, by immunofluorescence analysis, a decreased expression of IL-7 in the cytoplasm of IECs after I̸R was also confirmed compared with sham animals. Moreover, it has been reported that il-7rα̸CD127 may induce the phosphorylation of Stat5, which acts to maintain t-cell homeostasis and prevents the progression of inflammation. To better understand CD127 and the changes in phosphorylation of STAT5 in IELs after I̸R, flow cytometric analysis was performed and revealed that CD127 expression and the phosphorylation of STAT5 decreased after I̸R (CD127, 12.87±1.32%; and p-STAT5, 6.48±1.23%), as shown in Fig. 5. These data suggest that the IL-7̸CD127 signaling pathway is inhibited in IECs after I̸R, further aggravating the deterioration of intestinal barrier function.

IL-7 inhibits BMP4, which induces the apoptosis of IELs.
it is a known fact that the interaction between BmP4 and il-7 maintains the balance of proliferation and differentiation in cd34 + cells. To establish whether the interaction between BMP4 and IL-7 regulates the number of IELs, exogenous BMP4 (30 ng̸ml) and IL-7 (2 ng̸ml) proteins were added to cultured IELs for 6 h. flow cytometry demonstrated that treatment with il-7 reduced the extent of apoptosis of iels (11.1±0.93%), and enhanced the apoptosis of iels in the presence of BMP4 (11.8±0.47%). of note, no difference in the apoptosis of iels in the presence of BmP4 combined with il-7 stimulation was observed compared with the control group, as shown in Fig. 6. these results indicate that iec-derived il-7 signaling promotes the survival of iels and neutralizes iec-derived BmP4, which promotes the apoptosis of iels following I̸R.

IL-7 downregulates the BMP̸NF-κB signaling pathway in
IECs and in IELs. to determine how to delay the induction of IEL apoptosis by BMP4 in the presence of IL-7, the expression of BmP4 in iecs under il-7 protein (2 ng̸ml) stimulation was evaluated by western blot analysis. The expression level of BmP4 was found to be significantly decreased, as shown in Fig. 7A. Moreover, flow cytometry revealed that the expression of phosphorylated nf-κB protein was downregulated as a result of IL-7 (2 ng̸ml) stimulation in IELs in culture (5.18±1.12%),   . FITC-Annexin V + ̸PI + indicates late apoptosis, FITC-Annexin V + ̸Piindicates early apoptosis, and FITC-Annexin V -̸PIindicates live cells. the extent of apoptosis of IELs after treatment with BMP4 for 6 h was then determined. The expression of FITC-Annexin V + ̸PI + IELs in the BMP4 group was significantly higher compared with that in the control group, but these effects were decreased by treatment with NoggIN or pyrrolidine dithiocarbamate (PDTC). P<0.05, 3-4 mice̸group; each experiment was repeated three times. as shown in Fig. 7B. NF-κB signaling is known to increase the levels of inflammatory factors. it was hypothesized that il-7 downregulates BmP4 and the phosphorylation of nf-κB, thereby decreasing the expression of inflammatory factors to prevent the apoptosis of IELs.
BMP4 regulates the IL-7̸CD127 signaling pathway in IECs and IELs. The majority of studies have demonstrated the role of il-7 in t-cell homeostasis, in that cd127 induces Stat5 activation. The expression levels of CD127 were found to be highly downregulated in BmP4-treated cd34 + cells (15). therefore, it was hypothesized that BmP4 promotes the apoptosis of iels by downregulating cd127 in intestinal mucosal epithelial cells. Western blot analysis revealed that BMP4 (30 ng̸ml) decreased the expression of the IL-7 protein in IECs, as shown in Fig. 8A. The expression level of CD127 dictates the levels of il-7, which affect t-cell sensitivity (22). However, the expression of CD127 and the phosphorylation of Stat5 were downregulated in iels that were cultured in the presence of BMP4 (30 ng̸ml) (CD127, 7.30±0.48%; and p-STAT5, 4.77±1.77%), but these effects were abolished with the application of NoggIN (30 ng̸ml), as shown in Fig. 8B. therefore, it was hypothesized that BmP4 reduces il-7 and iel sensitivity through the downregulation of cd127 and the expression of phosphorylated STAT5, which would in turn negatively affect the number and function of IELs.

Discussion
the present study demonstrated that iec-derived BmP4 activates the nf-κB signaling pathway to promote apoptosis of iels following I̸R. IL-7 exerts these inhibitory effects by counteracting the actions of BMP4. Previous studies have investigated the roles of the BmP proteins in early intestinal development and in the proliferation and differentiation of IECs (32,33). We observed that the expression of BMP4 increases and that the expression of BMP receptors is also upregulated in IECs after hypoxia and I̸R. BMP4 then activates nf-κB signaling, which leads to increased levels of inflammatory factors, such as TNF-α and IL-6. These factors can destroy the intestinal mucosal barrier, weakening its function (12). IEC-derived IL-7 plays a crucial role in the control of the development and homeostasis of neighboring IELs (23). Previous studies investigated the regulation of the population of progenitor cells in the human thymus and indicated that the balance between signals occurs as a result of the interplay between the BMP and IL-7 signaling pathways (15). However, the association between the interaction of the BmP and il-7 signaling pathways and the regulation of iel apoptosis following I̸R has rarely been reported.
the BmP signaling pathway plays a critical role in inflammatory reactions (34,36). it was therefore hypothesized that the BmP signaling pathway contributes to the mechanisms that are involved in the promotion of apoptosis of IELs. the pres ent study investigated the expression of BMP receptors (BmPria, BmPriB, actria and BmPrii) and phosphorylated nf-κB protein in iels by rt-Pcr and flow cytometry. following I̸R, IECs produce BMP4 abundantly, and IELs express increased levels of BMPRIA, BMPRIB and phosphorylated nf-κB proteins. Thus, the evidence suggests that BmP may activate nf-κB signaling in IELs after I̸R. The addition of exogenous recombinant BMP4 to isolated and cultured iels was found to directly activate nf-κB signaling, and this effect was weakened by the addition of the BMP-specific antagonist NoggIN. it is known that an increase in the inflammatory cytokines IL-6 and TNF-α is mediated via the nf-κB signaling pathway (12) and, thus, flow cytometry was used and revealed that the addition of exogenous recombinant BMP4 promoted IEL apoptosis. This effect was attenuated in part by either the BMP-specific antagonist noggin or the nf-κB inhibitor PDTC. the present study provides new evidence that, following I̸R, BMP4 derived from IECs may bind to the BMP receptors on IELs. the phosphorylation of nf-κB allows for its translocation to the nucleus, so that it can trigger the transcription of genes that are involved in inflammatory cellular responses and other types of signaling (35). This, in turn, promotes the apoptosis of IELs. it was also recently confirmed that activating the canonical Smad signaling pathway promotes the apoptosis of IELs after I̸R. However, the underlying mechanism requires further elucidation.
the administration of iec-derived il-7 protein significantly affects the function and phenotype of IELs. The il-7-induced increase in the number of iels may be associated with the increased rate of iel proliferation observed by immunofluorescence; however, the expression level of the IL-7 protein was found to be decreased in iecs following I̸R for 6 h. IL-7 affects t-cell sensitivity through the expression of cd127, and it is possible that the availability of il-7 is limited in vivo (36,37). Moreover, IL-7 induces the phosphorylation Figure 6. Inhibition of bone morphogenetic protein (BMP)4 induces the apoptosis of intraepithelial lymphocytes (IELs) that has been stimulated by interleukin (IL)-7. flow cytometry and apoptosis markers (FITC-Annexin V and PI) were used to examine IEL apoptosis after treatment with BMP4, IL-7 or BMP4 + IL-7 for 6 h. The expression of FITC-Annexin V + ̸PI + IELs in the IL-7 treatment group was significantly lower compared with that in the control group, whereas the expression of FITC-Annexin V + ̸PI + IELs in the BMP4 treatment group was significantly higher compared with that in the control group. However, the expression of FITC-Annexin V + ̸PI + iels in the BmP4 + il-7 treatment group exhibited no changes compared with the control group. Each experiment was repeated three times; 4-5 mice̸group. P<0.05. Figure 7. Interleukin (IL)-7 downregulates the bone morphogenetic protein (BMP) signaling pathway in intestinal epithelial cells (iecs) and intraepithelial lymphocytes (IELs). (A) Western blot analysis was used to determine the expression of BMP4 in IEC-6 cells following treatment with IL-7 for 6 h. The expression of BMP4 was significantly decreased compared with that in the control group. (B) flow cytometry, was used to detect the phosphorylation of nuclear factor (nf)-κB following treatment with IL-7 for 6 h. The expression of phosphorylated NF-κB was significantly decreased compared with that in the control group. Each experiment was repeated three times; 4-5 mice̸group. * P<0.05 vs. control. gAPDH, glyceraldehyde 3-phosphate dehydrogenase.
of Stat5, which then translocates into the nucleus where it controls the expression of target genes that are involved in the survival and proliferation of T cells (38). It has been reported that BMP4 exerts an inhibitory effect on proliferation and differentiation and, thus counteracts the functions of il-7 in human intrathymic cd34 + precursor cells (15). These effects are mediated by BmP4-induced downregulation of cd127 expression in CD34 + precursor cells. CD127 deletion caused a 30% reduction in the numbers of cd4 + and cd8 + t cells, which confirms a key role for il-7r in the maintenance of peripheral t-cell survival (39). More importantly, other data directly indicate that BmP4 downregulates the level of cd127 and p-STAT5 expression in IELs in culture, which leads to a reduction in the sensitivity of IELs to IL-7. Therefore, BmP4 induces a decrease in the number of iels and weakens IEL function. Based on these data, it may be inferred that iec-derived BmP4 not only activates nf-κB signaling to induce iel apoptosis, but also downregulates cd127 and p-STAT5 protein expression to shorten the survival of IELs. iec-derived il-7 directly affects the number and function of iels; furthermore, it was observed that il-7 protein expression decreases following BMP4 stimulation. it may thus be hypothesized that the decreasing expression of IL-7 was associated with the increasing expression of BMP4 in IECs following I̸R. The reasons for this finding are not clear and require confirmation in future experiments.
the protein level of BmP4 in iecs, as well as the phosphorylation of nf-κB in iels, were found to be significantly downregulated in the presence of IL-7. The addition of exogenous il-7 markedly reversed BmP4-induced apoptosis of Figure 8. Bone morphogenetic protein (BMP)4 regulates the interleukin (IL)-7α̸CD127 signaling pathway in intestinal epithelial cells (iecs) and intraepithelial lymphocytes (IELs). (A) Western blot analysis determined the expression of IL-7 in IEC-6 cells following treatment with BMP4 for 6 h. The IL-7 level was significantly decreased compared with that in the control group. (B) flow cytometry, was used to detect the expression of CD127 and phosphorylated signal transducer and activator of transcription (Stat)5 proteins following treatment with BMP4 for 6 h. The levels of CD127 and phosphorylated STAT5 proteins were significantly decreased compared with those in the control group. Each experiment was repeated three times; 4-5 mice̸group. * P<0.05 for control vs. treatment with BmP4 alone; ** P<0.05 for control vs. the BMP4 + NoggIN group. gAPDH, glyceraldehyde 3-phosphate dehydrogenase.
IELs. As a result, it may be inferred that IL-7 exerts protective effects in terms of the number and function of iels through the downregulation of deleterious factors that are triggered by the BMP̸NF-κB signaling pathways. This protective effect decreases IEL apoptosis after I̸R, effectively preventing further deterioration of intestinal barrier function.
the regulation of nf-κB expression in developing T cells and the role of this pathway in selection and lineage determination is complex (16). Recent studies reported that basal nuclear nf-κB activity plays an important role in the transcription of cd127, which facilitates the responsiveness of naïve t cells to the prosurvival effects of il-7 and allows for t-cell persistence in vivo (40). Additionally, a developmental function has been reported for nf-κB signaling in the homeostatic maturation of new t cells via the regulation of il-7rα expression (41). our data revealed an increase the phosphorylation of nf-κB and decrease in IL-7̸CD127 protein expression in IELs after I̸R. However, whether there is an association between nf-κB and IL-7̸CD127 remains unclear. Hence, it is recommended that the association between nf-κB and IL-7̸CD127 is further investigated in a mouse model of I̸R.
To the best of our knowledge, the present study is the first to report that BMP4 expression was increased in IECs and that iec-derived BmP4 can directly activate nf-κB signaling and induce apoptosis of iels following I̸R. Additionally, IEC-derived il-7 was shown to downregulate BMP4 signaling after I̸R, which limits the extent of IEL apoptosis. This may result in the maintenance of the balance of the number of iels and their function following I̸R. All these effects indicate the importance of the interplay of BmP4 and il-7 and the dialogue between iecs and iels in the intergrity of the intestinal mucosal barrier.