The complete chloroplast genome of Epimedium davidii Franch. (Berberidaceae)

Abstract Epimedium davidii, which belongs to Berberidaceae, is mainly distributed in the southwest of China. In this study, the complete chloroplast genome of E. davidii was sequenced and assembled. The circular genome is 159,715 bp in length, which comprises a large single-copy region (LSC, 85,862 bp), a small single-copy region (SSC, 17,081 bp), and a pair of inverted repeat regions (IRa and IRb, 28,386 bp). The chloroplast genome of E. davidii contains 112 unique genes, of which 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis indicated that E. davidii was closely related to Epimedium acuminatum.

The Epimedium L. plants in the Berberidaceae family have been used as traditional Chinese herbs for more than 2000 years. The leaves of Epimedium, Epimedium folium (EF), exhibit beneficial pharmacological effects including reinforcing the yang, improving immunological function, and treating cardiovascular diseases, etc. (Ma et al. 2011;Liu et al. 2019Yang et al. 2019. Due to many controversies on the taxonomic and phylogenetic relationship of Epimedium family, more attention has been drawn to characterize the genetic diversity with modern molecular techniques (Li et al. 2015). DNA barcodes (rbcL, psbA-trnH, ITS, and ITS2) were used in identifying Epimedium species, but the classification and phylogeny of Epimedium family were still poorly solved (Guo et al. 2018). Besides, the application of chloroplast genome to identify species and determine phylogenetic relationship has been proven to be effective (Nguyen et al. 2015). Up to now, the complete chloroplast genome of five Epimedium species have been reported (Zhang et al. 2016;Liu et al. 2019), but more Epimedium species need to be further evaluated. In this study, we reported the complete chloroplast genome of E. davidii Franch. that is a unique species with some special floral appearance, aiming to provide more genomic information for the systematic and evolutionary studies.
In this study, E. davidii sample was collected from the Baoxing County of Sichuan province (China; 30 35 0 N, 102 52 0 E). A voucher specimen (18036) was deposited at the Herbarium of the Institute of Medicinal Plant (IMPLAD), Beijing, China. Total genomic DNA was extracted from the fresh leaves of E. davidii using the modified CTAB method (Doyle and Doyle 1987). The high-quality DNA was sheared to the size of 300 bp for the shotgun library construction.
The sequencing was performed on an Illumina Novaseq PE150 platform (Illumina Inc., San Diego), and 150 bp pairedend reads were generated. The filtered reads were assembled into the complete chloroplast genome using the program GetOrganelle version1.5 (Jin et al. 2018) with E. acuminatum chloroplast genome (GenBank accession number: NC_029941) as a reference. The annotation of the chloroplast genome was conducted through the online program CPGAVAS 2 (Shi et al. 2019), followed by manual correction if required. The annotated genomic sequence has been registered in GenBank with an accession number (MN621353).
To identify the phylogenetic relationship of E. davidii, 17 complete chloroplast genomes of Berberidaceae species were used to reconstruct a maximum-likelihood (ML) phylogenetic tree using RAxML version 8.2.10 (Stamatakis 2014), with Aconitum contortum as the outgroup (Figure 1). Phylogenetic analysis indicated that E. davidii is closely related to E. acuminatum. The complete chloroplast genome of E. davidii provides useful perspectives into the evolutionary patterns in Berberidaceae family.

Disclosure statement
No potential conflict of interest was reported by the authors.