Interactions of Cisplatin and Daunorubicin at the Chromatin Level

Unexpectedly, the widely used anticancer agents Cisplatin (Cis-Pt) and Daunorubicin (Dauno) exhibited cell type- and concentration-dependent synergy or antagonism in vitro. We attempted to interpret these effects in terms of the changes elicited by the drugs in the chromatin, the target held primarily responsible for the cytotoxicity of both agents. We measured the effect of Cis-Pt on the levels of Dauno in different cell compartments, the effect of Cis-Pt on Dauno-induced nucleosome eviction, and assessed the influence of Dauno on DNA platination in flow- and laser scanning cytometry as well as in laser ablation-inductively coupled plasma-mass spectrometry assays. We show that the two drugs antagonize each other through a decrease of interstrand crosslinks upon co-treatment with Dauno, and also via the diminished Dauno uptake in the presence of Cis-Pt, and both effects are observed already at low Dauno concentrations. At high Dauno concentrations synergy becomes dominant because histone eviction by Dauno intercalation into the DNA is enhanced in the presence of co-treatment with Cis-Pt. These interactions may have an impact on the efficacy of combination treatment protocols, considering the long retention time of DNA adducts formed by both agents.


cis-pt and Dauno synergize or antagonize each other in a cell-type and dose dependent manner.
Synergy between two drugs (when the observed effect is greater than the product of the effects of each individual agent), vs. antagonism (when the observed effect is smaller than what is expected when the effects add up), are not readily determined by comparison of the individual effects with those of the combinations (Fig. S1), due mainly to the non-linearity of the dose-effect relationships. Therefore, we used the combination index (C.I.) method based on the median-effect principle of the mass-action law 26 , performed according to Chou-Talalay (see also Materials and Methods), to determine the mode of drug interaction. Cytotoxicity was evaluated by performing a Resazurin cell viability assay. Our initial measurements of cytotoxic interactions between Dauno and Cis-Pt added together to Jurkat cells confirmed the general synergism of the two drugs over a wide concentration ratio, what is expected in view of clinical experience 1,2 exhibited best at 40 uM Cis-Pt for both low and high Dauno concentration (Fig. S2). Unexpectedly, in sharp contrast with the Jurkat cells, in HeLa cells antagonism was observed (C.I. > 1) at the subtoxic Dauno concentration range of 0.3-1.8 μM, and synergism (C.I. < 1) at toxic Dauno concentrations ranging between 3-12 μM, using 40 μM Cis-Pt ( Fig. 1 and supplementary Table 2). The phenomenon of dose-and cell line-dependent synergism vs. antagonism is not unique to Jurkat and HeLa cells (e.g. T47D cells exhibited antagonism of the two drugs at high, and synergy at low Dauno concentration; data not shown). It was also not unique to Dauno, since Doxorubicin (Dox), another anthracycline closely related to Dauno also showed such a varied response (data not shown). We set out to investigate the possible scenarios of interactions that may lead to synergism or antagonism.
Co-treatment with Cis-Pt differentially alters the amount of total cellular, chromatin-bound and DnA-bound Dauno. We first evaluated whether the antagonism between the two drugs might be related to an effect of Cis-Pt on the amount of Dauno taken up into the cells, accumulating in the acidic endosomal compartment 27 , by assessing Dauno fluorescence in live cells using flow-cytometry. In parallel, the total nuclear fluorescence of Dauno, accounting for the chromatin-bound drug, was also measured. Dauno fluorescence was significantly decreased in Cis-Pt-Dauno co-treated cells as compared to cells incubated with Dauno only, as measured both in live cells (Fig. 2a) and nuclei (Fig. 2b). Since Dauno also binds to histones 28 in addition to DNA, we performed an assay to directly measure the effect of Cis-Pt co-treatment on DNA-bound Dauno in live cells via the quenching of Hoechst 33342 fluorescence 29 . Hoechst 33342 enters live cells and becomes brightly fluorescent upon binding through the minor groove of the DNA to tandem AT base pairs [30][31][32] . Its green emission is absorbed by Dauno quenching Hoechst fluorescence what can be utilized as a measure of DNA-bound anthracycline. The decrease of Hoechst fluorescence in the presence of Dauno was stronger when Cis-Pt was also added. This difference can be partly accounted for by the influence of Cis-Pt on Hoechst fluorescence (see Table inset of Fig. 2c). However, an increased level of DNA-bound anthracycline in the presence of Cis-Pt co-treatment was corroborated in experiments when the DNA of cells treated with Dox (an anthracyclin closely related to Dauno) was isolated, run on agarose gels and visualized by ethidium bromide (EBr) staining, see Fig. S3). Thus, in view of the fact that DNA-bound anthracycline levels certainly did not decrease (rather increased based on Fig. S3), the decreased nuclear fluorescence of Dauno in the presence of Cis-Pt (Fig. 2b) may reflect a significantly decreased presence of histone-bound Dauno levels, likely due to histone eviction elicited by the anthracycline. In summary, Cis-Pt decreases the amount of anthracycline accumulating in the cytoplasmic acidic compartment (comprising the dominant species of the drug), it also decreases the amount of the histone-bound Dauno, while the amount of DNA-bound anthracycline increases.  Detection of ICLs using a modified alkaline comet assay. Another possible mode of interaction between the two classes of drugs is relaxation of supercoiling by the intercalator, mitigating Cis-Pt-DNA ICL formation 20 . In order to study this possibility, we employed a modified version of the alkaline comet assay where the ICL-dependent increment in the renaturation of alkali-denatured samples of nuclei is measured 24 . In view of the rather high background of renatured DNA in the absence of crosslinker in that procedure (Fig. 4a), we used S1 nuclease treatment to remove any non-renatured DNA, and the SYBR Green I dye to stain ds DNA (Fig. 4c,d). Using these modifications and employing LSC to measure the fluorescence of nuclear comets (or, with equivalent results, nuclear halos; see Materials and Methods), ICL detection was made possible in a wide concentration range of Cis-Pt added to live cells, above ~5 μM (Fig. 4e). In the subsequent experiments we used this modified assay to detect ICLs.
Co-treatment with Dauno influences Cis-Pt-DNA adduct levels. In order to assess the effects of Dauno intercalation on the formation of Cis-Pt-DNA adducts, nuclei of HeLa cells were exposed to combinations of 40 μM of Cis-Pt and Dauno used at various concentrations. ICL levels were assessed employing the modified comet assay described above (Fig. 4), while total Cis-Pt DNA adducts were measured via immunofluorescence using an antibody recognizing all Cis-Pt-DNA adducts. We observed no change in the level of total DNA-Pt adduct levels (Fig. 5a-c), while there was a significant reduction in ICL formation ( Fig. 5b-d). Notably, this effect was detected already in the low concentration range of the intercalator.

Effect of nucleosome eviction on Cis-Pt-DNA adduct levels. Dauno can cause an increase of total
Cis-Pt-DNA adduct levels as well as of ICLs due to increased accessibility of the DNA in the wake of nucleosomal eviction. ICL levels may be increased just because total adduct levels are increased. On the other hand, they can be decreased as a result of the relaxation of the superhelical twist accompanying intercalation 12 and also as a result of nucleosome eviction allowing for free twist-writhe interconversion. In view of the complexity of possible scenarios of drug interactions, a model system was set up to learn how nucleosome eviction, in the absence of any other effects of Dauno, might influence Cis-Pt binding to the DNA. We measured total Cis-Pt adduct levels and ICLs in nuclei pre-treated with salt to remove the dimers (1.1 M NaCl), or all the histones (1.6 M NaCl). Total adduct levels were assessed by three independent methods: by immunofluorescence using the anti-Cis-Pt antibody (Fig. 6b), by measuring the fluorescence of ds DNA in the renatured samples when S1 digestion step was omitted so as to detect all the Cis-Pt adducts renaturing into ds DNA when (any) two strands are covalently linked by Cis-Pt (Fig. 6a), and also by LA-ICP-MS (Fig. 6d). ICLs were measured by the modified comet assay described above (Fig. 6c) as well as by LA-ICP-MS following the treatments performed in the comet assay (Fig. 6e). As Fig. 6 shows, the different approaches gave similar results. Total DNA platination was increased at intermediate salt levels only, while ICLs were decreased, moderately at 1.1 M, and more strongly at 1.6 M salt concentration.  www.nature.com/scientificreports www.nature.com/scientificreports/

Discussion
We have observed a cell type-and dose-dependent interaction between Dauno and Cis-Pt (see Fig. 1 and supplementary Table 2), which warrants a detailed analyses of the mechanistic components of the interactions in the case of their combined application in cancer chemotherapy. Reminiscent of our findings, cell type and dose-dependent synergism vs. antagonism was described for the combination of Cis-Pt with a deoxycytidine analogue 33 . In view of the fact that both Dauno and Cis-Pt appear to primarily target the chromatin 5,15 , we set out to assess how they affect each other's binding to particular cellular components, focusing on the chromatin. There is little doubt that the mode of Cis-Pt action most relevant to cytotoxicity is its binding to the DNA 15 . Although the effects of Dauno are more diverse, its intereaction with the different components of the chromatin are generally considered of primary importance for its toxicity 7 . Also in line with the hypothesis that Dauno exerts its cytotoxicity mainly in the nucleus, a shift in its cellular distribution was observed toward higher cytoplasmic/ nuclear ratios in resistant cells 34 .
Regarding the effect of Cis-Pt co-treatment on Dauno levels (Fig. 2a), total Dauno fluorescence representing mainly the acidic endosomal compartment where amphiphylic compounds spontaneously accumulate, decreases upon co-treatment. Therefore, if extranuclear effects are also important factors in Dauno toxicity, Cis-Pt could antagonize them by reducing the cytoplasmic Dauno levels. The mechanism of decreased uptake was not further investigated in this study focusing on possible chromatin level interactions. Cis-Pt also decreased total nuclear Dauno fluorescence (Fig. 2b). Dauno binds more avidly to DNA than to histones 35 , but its fluorescence is quenched only by DNA 36 . Therefore, the fluorescence intensities measured probably reflect the presence of both Dauno-DNA and Dauno-histone complexes, but in an unknown ratio. Therefore, we applied an assay that can www.nature.com/scientificreports www.nature.com/scientificreports/ directly measure DNA-bound Dauno, based on the quenching of Hoechst 33342 fluorescence by Dauno when they are in molecular proximity 29 . The data of Fig. 2c and Fig. S3 reveal that the levels of DNA-bound Dauno do not decrease (Fig. 2c), rather increase (Fig. S3) upon Cis-Pt co-treatment, so the diminished total nuclear fluorescence is likely due to decreased amounts of histone-bound anthracycline. As Fig. 3 shows, Dauno-induced eviction of histones was augmented in the nuclei co-treated with Cis-Pt. Thus, the decreased nuclear presence of Dauno in the Cis-Pt co-treated samples (see Fig. 2b) is possibly due to the augmented histone release. Cis-Pt is known to cause distortions in the DNA structure which may be an important factor in its influence on Dauno-induced histone eviction: DNA containing a Cis-Pt 1, 2-intrastrand d(GpG) adduct is bent by 60° toward the major groove 37 . Eviction occurs also when live cells are treated with anthracyclines in line with references 9,12 and it is also augmented by Cis-Pt co-treatment as shown in Fig. 3b. The Cis-Pt elicited increase in the eviction of H2B was detected already at low, while H3 eviction was augmented only at higher Dauno concentrations. Thus, interaction of the two drugs in terms of histone eviction appears to be synergistic in the whole concentration range of the anthracycline. In summary, Cis-Pt augments the binding of anthracyclines to the genomic DNA in spite of the overall decrease of cellular uptake in its presence (Figs. 2 and S3). In view of the increased histone eviction in the presence of Cis-Pt (Fig. 3) this may be a consequence of the appearance of DNA regions lacking topological constraints (restricting intercalation). Cis-Pt-augmented histone eviction was observed in HeLa cells at low as well as high Dauno concentration, and Cis-Pt in HeLa cells increased the DNA-binding of Dauno applied at low concentration (Fig. 2), formally ruling out the possibility that these factors might play a role in the antagonism found in HeLa cells at low Dauno concentration. On the other hand, the augmented formation of www.nature.com/scientificreports www.nature.com/scientificreports/ covalent Dauno-DNA adducts in the case of treatment with a combination of Dauno and Cis-Pt (Fig. S3) may contribute to the synergistic toxicity of the two drugs.
Regarding the effect of Dauno on Cis-Pt-DNA adduct levels, total platination was not affected (Fig. 5a), while the level of ICLs, assessed using a modified version of the alkaline renaturation assay ( Fig. 4; see also Materials and Methods), was significantly decreased (Fig. 5b). The decreased ICL levels observed at low Dauno concentration were not further changed using higher concentrations of the anthracycline; thus, the antagonistic effect of Dauno on ICL formation appears to be similar in the entire Dauno concentration range tested. Therefore, if ICLs significantly contribute to Cis-Pt toxicity, Dauno co-treatment could mitigate these consequences in the low Dauno concentration range, while the synergistic other effects may overrule this at higher concentrations. The decrease of ICL formation is likely due to relaxation of negative supercoiling of the chromatin loops, in line with reference 20 and with data on psoralen cross-linking 21 . Based on the above, the antagonism of low Dauno concentration with Cis-Pt in HeLa cells is suggestive of the importance of ICL formation in the cytotoxicity of Cis-Pt in certain experimental and treatment scenarios.
We have set up a model system comprising isolated nuclei treated with moderate and high salt concentration, to evict nucleosomes and elicit topological changes of the DNA in a controlled manner and in the absence of topological changes induced directly by intercalators. The LA-ICP-MS approach let us obtain absolute numbers for the total adduct levels and for ICLs and these are comparable to the numbers determined in other studies 17 . The magnitude and direction of changes in total platination levels (mainly intrastrand cross-links 38 ) and ICLs were comparable to the changes observed when nuclei were treated with Cis-Pt and Dauno (Fig. 5). The Eviction of nucleosomes by salt and Dauno alike increases the target size for Cis-Pt binding. Furthermore, intrastrand crosslinks that involve bending of the DNA 22,23 must be severely antagonized by any constrainment of its structure and augmented by the release of such constraints. On the other hand, the increase of free DNA regions upon nucleosome eviction is expected to favor formation of highly constrained, hyperplectonemic structures likely to occur in the case of long superhelical DNA molecules 39 . These antagonistic effects can explain the changes observed in total Cis-Pt-DNA adduct formation in our model system (Fig. 6). The increase of target size upon histone eviction may be dominant at moderate salt concentration, increasing adduct levels, while the hyperplectonemic constrainments in the naked DNA produced by high salt pre-treatment may prevent the bending of DNA required for intrastrand crosslinking 22,23 . On the other hand, ICL formation is strongly favored by negative Tw 20 . The superhelicity of the linker regions is generally assumed to be negative 40 . An ~30% of the overall linking number difference is estimated to partition as twist 41 . The nucleosomal structure entails slightly overwound DNA (Tw = 10.3 bp/turn compared to 10.5 bp/turn for B-DNA 42 . The negative twist in the linker regions together with the slightly positive twist inside the nucleosomes can account for the linker-preference of ICL formation 16 . At moderate salt treatment, upon eviction of H2A and H2B 12 , the enlarged nucleosome-free regions are expected to adopt a topology with higher degree of freedom to redistribute twist and writhe. As a consequence, Wr likely becomes more, and Tw less negative in the widening linker regions, in comparison with their state before eviction. Such changes would lead to a decrease in ICL numbers after treatment with 1.1 M salt. At high salt, upon complete eviction, the hyperplectoneme imposed topological constraints would antagonize proper apposition of the bases involved in ICL formation, further decreasing the chances of their generation. The data obtained in this model system are in line with the interpretation that the Dauno evoked decrease of ICL formation observed in isolated nuclei (Fig. 5) is, at least partly, due to Dauno induced eviction of the histone dimers (Fig. 3). To what extent Dauno-elicited Tw relaxation directly contributes to a decreased ICL formation could not be determined because nucleosome eviction necessarily accompanies intercalation. The scenario of complete eviction modeled by high salt concentration pre-treatment of isolated nuclei does not occur even at high Dauno concentrations. However, eviction may not be uniform along the genome, affecting certain chromatin regions more than others. Local stretches of naked DNA may be present when live cells are treated with Dauno. The formation of ICLs is expected to be reduced in these regions based on the data of Fig. 6. In summary, histone eviction by itself is expected to diminish ICL formation, while total adduct formation may not change due to the complexity of effects involving increased target size represented by a more relaxed naked DNA on the one hand and constraints emerging in the wake of DNA-DNA interactions upon hyperplectoneme formation on the other. These observations on the model system are in line with the effect of Dauno on total Cis-Pt adduct formation and on ICL formation (Fig. 5).
Since both drugs have multiple targets in the cell, antagonism as well as synergy could involve multitudes of factors. However, as both drugs are considered to primarily target the chromatin 15,35 , their interactions here may be important determinants of the pharmacological outcome of co-treatment. It remains to be determined if inhibition of Top II by Dauno, which was initially perceived as the primary cytotoxic effect of anthracyclines 43 , influences Cis-Pt toxicity. However, apoptosis induction by the DNA-Dauno adducts rather than Top II inhibition appears to be the crucial factor in the cytotoxicity of anthracyclines 44 .
In conclusion, as summarized in Supplementary Fig. S6, Dauno and Cis-Pt antagonize one another through a decrease of ICLs in the presence of Dauno (at its low and high cc. alike), and also via the diminished Dauno uptake in the presence of Cis-Pt. They could synergize with each other through enhanced histone eviction by Dauno in the presence of co-treatment with Cis-Pt, accompanied by an increment of DNA bound anthracycline. These drug interactions have not been described before to the best of our knowledge and may impact cytotoxicity reached by combination treament regimens in vivo.

Materials and Methods
All reagents were purchased from Sigma-Aldrich (St. Louis, Missouri, USA), unless otherwise stated. www.nature.com/scientificreports www.nature.com/scientificreports/ (Corning Glass Works, Corning, NY) in 5% CO 2 . Treatment with cytotoxic agents was performed in 24-well plates. Dauno (Pfizer, Budapest) was used in a concentration range of 0-15 μM. Cis-Pt (Accord-UK Ltd, United Kingdom, purchased as 1 mg/ml stock solution) was applied at 0-80 μM. For median effect analyses, Cis-Pt was added to the cells at 40 μM final concentration together with a concentration series of Dauno, for 16 hours. Cytotoxicity was measured based on mitochondrial function, assessed using the Resazurin based assay 45 . 100 μl aliquots of the drug treated cells were resuspended in colorless media and added to 96-well flat-bottom microplates to 20,000 cells per well. For each drug combination four parallel wells were prepared. 100 μl of freshly prepared Resazurin solution in colorless medium was added to each well to a final concentration of 18 µM. The plates were incubated at 37 °C for 24 h and fluorescence signals were measured at an excitation wavelength of 560 nm and an emission wavelength of 590 nm using Synergy H1 microplate reader (BioTek). Viability was expressed in the experimental groups in relation to untreated cells (control). The average concentrations of four wells were determined per dose. To determine synergism, additivity or antagonism, median effect analysis was performed using the method of Chou-Talalay 46 , using CompuSyn ver. 1.0 (ComboSyn Inc., Paramus, NJ, USA). The CI (combination index) reflects synergism, additivity or antagonism when CI < 1; CI = 1 or CI > 1, respectively. All indicated values are the averages of at least three independent experiments. The method involves recording of dose-response curves for the individually applied drugs, and measurement of the cytotoxic effect at a fixed dose of one drug, varying the concentration.of the other. Typical dose response curves are demonstrated in Fig. S1.

Measurement of icLs and total cis-pt-DnA adduct levels by LSc. Preparation of comets and
halos. Following exposure to drugs, the cells were centrifuged at 175 g for 5 min at 4 °C, then resuspended to a final concentration of 1 × 10 6 cells/ml in PBS, kept at 37 °C and mixed at a v/v ratio of 1: 3 with 1% Low melting point agarose dissolved in PBS. 100 μl of the cell-agarose suspension was pipetted onto Superfrost microscope slides (Thermo Fisher Scientific, Waltham, Massachusetts, USA) and were covered with coverslips (VWR, Hungary, 60 mm × 24 mm). The slides were pre-coated by submerging them in molten 1% agarose (SeaKem ® LE Agarose, Lonza) dissolved in water, and then allowed to dry overnight at room temperature. The cells were left to sediment on the surface of the slides for 4 minutes at 37 °C, then kept on ice for 5 min. After polymerisation of the agarose, the coverslips were gently removed, then the slides were submerged in glass staining tanks containing ice-cold PBS/EDTA for 5 minutes, then submerged in ice-cold TRIS/EDTA (Tris-HCl 20 mM pH = 7.5, 5Mm EDTA), 150 mM NaCl and 0.1% v/v Triton X-100 for 10 minutes, next in PBS/EDTA containing 1% Triton X-100 for another 10 minutes. After washing in PBS/EDTA for 10 minutes, the samples were equilibrated with nickase buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 10 mM MgCl 2 , 1 mg/ml BSA), then nickase treatment was performed covering the slides with 500 μl of nickase buffer containing the frequent cutter nicking endonuclease Nt.CviPII (New England Biolabs Inc., Ipswich, MA, USA) at a final concentration of 0.001 U/ml and incubated in a wet chamber at 37 °C, for 20 min. After washing with ice-cold PBS/EDTA three times for 10 minutes, the slides were submerged in freshly made alkaline lysis buffer (2.5 mM NaCl, 1% N-Laurylsarcosine sodium salt, 10 mM Tris, 150 mM NaOH, 100 mM EDTA, 10% DMSO, and 1% Triton X-100; pH = 10) at room temperature, for 1 h. After alkaline denaturation, the slides were placed in an electrophoresis tank (Cleaver Scientific Ltd, United Kingdom) filled with alkaline running buffer (300 mM NaOH, 1 mM EDTA, pH = 13). After electrophoresis (for 20 min at 200 mA), the samples were neutralized with 0.4 M Tris (pH = 7.5) for 10 min before staining with SYBR Green I (Thermo Fisher Scientific, Waltham, Massachusetts, USA), 1: 5,000 diluted in PBS/EDTA. Since no background fluorescence was detected following S1 digestion (see Fig. 4), simple intensity measurements instead of calculating tail-moments sufficed, so the steps related to comet formation (nickase digestion and electrophoresis) were omitted and the experiments were performed in ibidi 8-wells microslide. These halo preparations allowed comparison of samples in ibidi slides that proved to be more convenient and reliable, improving also throughput. (2020) 10:1107 | https://doi.org/10.1038/s41598-020-57702-7 www.nature.com/scientificreports www.nature.com/scientificreports/ Detection of ICLs. Non-renatured DNA of the comets and halos prepared as above were removed by S1 nuclease digestion. The samples were equilibrated with S1 buffer (280 mM NaCl, 50 mM CH 3 COONa and 4.5 mM ZnSO 4 , pH = 4.4) for 30 min, then the samples were covered with 500 µl of S1 enzyme solution. The enzyme (Thermo Fisher Scientific, Waltham, MA, USA) was diluted to 1000 U/ml concentration in S1 nuclease buffer in the working solution. The samples were incubated on ice for 5 min with the enzyme for equilibration, then digestion was performed at 37 °C, for 20 min. After washing in PBS/EDTA, the samples were stained with SYBR Green I as described above, for 1 h.
Determination of total amount of Cis-Pt-DNA adducts. Nuclear halos were labeled by indirect immunofluorescence using anti-Cisplatin primary antibody as described below. Agarose-embedded nuclei on ibidi slides were exposed to the combinations of Cis-Pt and Dauno at 4 °C for 16 hours, washed with ice cold PBS/EDTA three times for 5 minutes each, treated with Proteinase K (Thermo Fisher Scientific, Waltham, Massachusetts, USA) dissolved in 0.4 M EDTA, 1% Sodium lauroyl sarcosinate, 10 mM Tris, pH = 8 at 0.5 mg/ml final concentration, at 42 °C, for 24 h. The enzymatic reaction was stopped by incubating the samples with 1:10 dilution of 10 mM PMSF) at room temperature, for 10 minutes. Then the samples were denatured and renatured as described above; electrophoresis was skipped. Immunofluorescence labeling was performed at 4 °C, overnight, using rat monoclonal anti-Cisplatin modified DNA antibody (Abcam, Cambridge, UK; 1 mg/ml) diluted at a titer of 1:800 in 150 µl of PBS/EDTA/1% BSA. Samples were washed 6 times for 10 min each, followed by incubating the samples with 500 μl 5% (m/v) Blotto Non-Fat Dry Milk (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) in PBS/ EDTA on ice for 30 min. Labeling with the secondary antibody was performed using Alexa Fluor 488 (A488) conjugated Donkey anti-rat IgG (Thermo Fisher Scientific, Waltham, Massachusetts, USA), at a titer of 1:800, diluted in PBS/EDTA/3% BSA, from 2 mg/ml stock solutions. After labeling with the secondary antibody, nuclei were washed with 500 μl ice-cold PBS/EDTA 3 times for 10 min each. Then the samples were fixed using 1% formaldehyde at 4 °C, overnight. After fixation, the nuclei were washed with 500 µl ice-cold PBS/EDTA three times for 5 min and fluorescence intensity distributions were recorded by LSC. For studying the effect of salt induced histone eviction on total Cis-Pt-DNA adduct formation (Fig. 6a), the agarose embedded nuclei of HeLa cells were prepared on the slides as described above and were treated with 1.1 M or 1.6 M of NaCl in PBS/EDTA 6 times for 10 min each and subsequently washed with PBS/EDTA. These samples were exposed to 40 µM of Cis-Pt, for 16 hr, then treated with proteinase K followed by PMSF and washing steps, then labelled by immunofluorescence for the LSC measurements, as described above. Then the same samples were dehydrated with an ascending series (20-95%) of ethanol for subsequent LA-ICP-MS analysis.
Laser scanning cytometry (LSc). Automated microscopic imaging was performed using an iCys instrument (iCys ® Research Imaging Cytometer; CompuCyte, Westwood, MA, USA). Green fluorescent protein (GFP), SYBR Green, A488, and propidium iodie (PI) were excited using a 488 nm Argon ion laser. A647 was excited with a 633 nm HeNe laser. The fluorescence signals were collected via an UPlan FI 20 × (NA 0.5) objective. GFP and A488 and SYBR Green fluorescence were detected through 510/21 nm, 530/30 nm and 550/30 nm filters respectively, while A647 and PI were detected through a 650 nm/LP filter. Each field (comprising 1000 × 768 pixels) was scanned with a step size of 1.5 μm. Data evaluation was performed using the iCys 7.0 software for Windows XP. Gating of G1 phase cells was based on the fluorescence intensity distribution curves of the DNA stained with PI.

Determination of pt in the nuclei of agarose-embedded cells using quantitative laser ablation-inductively coupled plasma-mass spectrometry spot analysis. LA-ICP-MS instrumen-
tation. An Analyte G2 193 nm ArF* excimer-based LA-unit (Teledyne Photon Machines Inc., Bozeman, MT, USA), equipped with a low-dispersion Cobalt ablation cell, was coupled to a quadrupole-based Agilent 7900 ICP-mass spectrometer (Agilent Technologies Inc., Tokyo, Japan) via the ARIS (Aerosol Rapid Introduction System, Teledyne Photon Machines Inc., Bozeman, MT, USA), providing fast aerosol transport [47][48][49] . For preparation of external calibration standards, instrument settings and data acquisition conditions see supplementary methods and supplementary Table 1.
Spot analysis. The determination of Pt in the nuclei of agarose-embedded cells was performed using a quantitative LA-ICP-MS spot analysis approach, derived from the single-cell LA-ICP-MS analysis method described in a previous work by Van Acker et al. 50 . See supplementary methods for details.