Quercetin loaded folate targeted plasmonic silver nanoparticles for light activated chemo-photothermal therapy of DMBA induced breast cancer in Sprague Dawley rats

Currently, the paucity of free drugs in conventional chemotherapy for breast-cancer curbs the desired therapeutic efficiency, often aggravating systemic toxicity. Quercetin (QRC) is a potential chemotherapeutic bio-flavonoid that is associated with poor hydrophilicity. In contrast to spherical silver nanoparticles (AgNPs), anisotropic AgNPs exhibit prominent plasmonic tunability in the near infrared (NIR) region allowing deep tissue penetration and endowing them with the ability to act as photothermal transducers as well. In this study, we optimized a simple and novel method for synthesizing folate-receptor-targeted-plasmonic silver-nanoparticles (QRC-FA-AgNPs) to serve as an efficient nanoscopic carrier system for breast cancer-cell targeted delivery of QRC and to induce photothermal therapy. A one-pot chemical synthesis method was followed for synthesizing the QRC-FA-AgNPs by finely tailoring the hydrogen bond between the reductant and stabilizer. Detailed characterization through UV-visible, near infrared (UV-vis-NIR) spectroscopy, Fourier transform infrared (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and energy-dispersive X-ray spectroscopy (EDX), along with particle-size, zeta-potential analysis, drug-loading and release capacity and stability studies were also performed. In vitro targeted cellular uptake, viability studies, chemo-photothermal efficacy, induction of apoptosis and the reactive oxygen species (ROS) generating potential were studied in the MDA-MB-231 cell-line and in vivo evaluation of the chemo-photothermal efficacy of QRC-FA-AgNPs was performed using a 7,12-dimethylbenz(a)anthracene (DMBA)-induced breast-carcinogenesis model in Sprague Dawley rats. Unlike conventional AgNPs, these novel pentagonal QRC-FA-AgNPs (<50 nm) manifested a robust plasmon tunability in the NIR (>800 nm) region. Detailed in vitro and in vivo studies revealed their active role in improving breast-cancer conditions by allowing controlled and targeted discharge of QRC at the tumor site, along with evoking hyperthermia under NIR laser irradiation that induced selective ablation of cancer cells. Following successful cellular internalization, the photothermal efficacy of QRC-FA-AgNPs supplemented their chemotherapeutic potency, allowing apoptosis and restraining the tumor growth. This current study highlighted the augmented efficacy of plasmonic QRC-FA-AgNPs in comparison to free quercetin, thus the development of a potential nanocarrier based on the pleiotropic function of plasmonic AgNPs may provide an efficient combined chemo-photothermal based strategy for the assassination of breast-cancer cells.


Introduction
Breast cancer, categorized as one of the most frequent and prevalent malignancies among women, is the second leading cause of mortality following lung cancer. In 2019, it was estimated that about 268 600 new breast cancer (invasive) cases were diagnosed in women, with 41 760 expected to result in death. 1 The limitations of conventional chemotherapy characterize it as an unsatisfactory therapeutic strategy for such a lifethreatening disease. Poor biodistribution of the standard chemotherapeutic agents, along with their non-specic tumor delivery result in undesirable side effects to normal tissues and fosters an inadequate dosage to destroy the cancer cells. 2,3 Moreover, as a greater amount of cancer cells are being recognized as being multidrug resistant, the insufficiency of the single therapy mode is becoming more apparent. Such a scenario demands prompt use of targeted [4][5][6] and combined treatment options [7][8][9] to improve therapeutic efficiency and biosafety for the treatment of breast cancer. This paradigm shi has thus created a profound impact on the current research that now focuses on the discovery of newer bioactive phytoconstituents and advances in the eld of nanotechnology. In this context, nanoparticles, as efficient drug delivery cargos, serve as an encouraging anti-cancer therapeutic approach.
Quercetin (QRC), a potent bioavonoid which is abundantly available in plants and in the human diet (e.g. onion, lettuce, tea, red grapes, tomatoes etc.), is reported to possess diverse pharmacological properties, as well as toxic effects towards various cancer cells. [10][11][12] Studies have documented QRC to inuence a number of key effectors in different signal transduction cascades associated with intrinsic apoptosis 13 and cell cycle arrest in breast cancer cells. 11,13 However, the insufficient aqueous solubility of QRC accounts for its poor bioavailability (20%, as shown by its water-soluble derivative), which in turn impedes its chemotherapeutic potential and also means that higher doses of QRC need to be administrated for clinical application. 12,[14][15][16] To surpass these obstacles and further enhance the efficacy of QRC, nano-formulations may be improvised to provide a successful platform for combining multiple therapeutic and/ or diagnostic modalities. 17 The nanoscale size (smaller than human cells by approximately 100-1000 times) facilitates their interactions with various biomolecules, which contributes to the unprecedented outcome of accurate therapy, as well as diagnosis. 18 Anisotropic silver nanoparticles (AgNPs), among different relevant nanoparticles (NPs), have gained signicant attention owing to their robust plasmon tunability in the biologically transparent window of 650-1200 nm. 19 According to various studies, AgNPs have been identied to be genotoxic and are also attributed to antitumor activities in vitro and in vivo. 20,21 AgNPs are also responsible for the selective killing of cancer cells by reactive oxygen species (ROS) induced apoptosis (mitochondria dependent). 22,23 Moreover, the efficiency of AgNPs in cancer therapy is signicantly escalated by virtue of its photothermal effect. 24,25 Distinctive near infrared (NIR) excitation from a laser source ensures superior tissue permeability and promotes luminous-to-heat conversion at the tumor region that invokes hyperthermia (>41 C) and is termed photothermal therapy (PTT). 24,26 The resultant heat allows selective killing of tumor cells without damaging healthy tissues because of the variation in the thermal tolerance between them. 25 PTT is therefore an effective anticancer therapeutic modality owing to its non-invasive properties. [27][28][29] In recent years, extensive nanoscale research has been primarily focused on unveiling the efficiency of diversied engineered nanoparticles including molybdenum disulde (MoS 2 )-keratin nanosheets, 30 gold coated liposomes, 31 reduced graphene-polyallylamine hydrochloride nanocomposites, 32 nanoceria-molybdenum nano-akes, 33 and so forth for inducing and improving photothermal therapy against liver, breast and other cancers. Thus, these "optically hot" anisotropic AgNPs can serve as a multifunctional drug delivery system facilitating combined chemophotothermal therapy. As folate receptors (FR) are highly overexpressed in breast and other cancers, 34 with respect to normal tissues, folic acid (FA) is therefore considered to be a tumor targeting molecule for breast cancer theranostics owing to its high binding affinity towards FR. Accordingly, developing a high performance FA functionalized nanocarrier may authorize targeted cancer cell endocytosis and accumulation allowing superior biocompatibility for practical utilization of AgNPs based PTT for breast cancer.
Thus, in contrast to the complex multistep methods previously reported, in this study, we optimized a simple, novel and one-pot chemical synthesis technique for formulating a FR targeting silver based plasmonic nanocarrier for escalating the potency of QRC. Also, detailed in vitro and in vivo investigations were performed to evaluate the breast cancer specic cell targeting ability with the combined chemo-photothermal efficacy of QRC-FA-AgNPs against breast cancer. Among the various preclinical rodent models explored for breast cancer studies, we opted for DMBA (7,12-dimethylbenz(a)anthracene), a prototype of polyaromatic hydrocarbons (PAH), induced breast cancer model on Sprague Dawley rats 35,36 for the in vivo evaluations.

Synthesis of plasmonic anisotropic AgNPs
The silver nanoparticles were synthesized according to the method described previously. 19 Briey, initial mixing of the AgNO 3 (1 mM, 10 ml) and citrate (80 mM, 0.5 ml) solution was followed by the addition of hydrazine hydrate (40 mM, 2 ml), the solution was maintained at pH 5 and a temperature of 5 C with constant stirring until a green color appeared. The pH adjustment of the solution was carried out by adding 1 M NaOH or 1 M H 2 SO 4 dropwise.

Synthesis of folate receptor targeted AgNPs (FA-AgNPs)
In this study we performed a novel and simplied method of folic acid conjugation with AgNPs. The pH of the previously synthesized silver nanoparticle (AgNP) solution (12.5 ml) was adjusted to a pH of 7.6 before adding folic acid solution (160 mM, 0.5 ml) under constant stirring. Aer that the solution was allowed to rest for 2 h followed by freeze drying to concentrate it to at least one h of the previous volume. Isopropyl alcohol (IPA, 10 ml) was added to the sample and then it was centrifuged at 4500 rpm for 10 min. The resulting supernatant was decanted and the obtained pellets were again centrifuged with IPA under similar conditions as previously stated. Aer the second washing, pellets of FA-AgNPs were collected and lyophilized to a powdered form for further use and characterization. better solubility) and the pH was adjusted to 11. 0.5 ml of this solution was added to the previously synthesized AgNPs (12.5 ml) which had also been adjusted to a similar pH as that of the QRC + FA solution under constant stirring. Then, aer a 2 h resting period, the prepared solution was freeze dried following a previously described method for FA-AgNPs. Aer being adequately concentrated the solution was centrifuged by adding IPA and washed twice as described previously to obtain the nal pellets of QRC-FA-AgNPs. These pellets were then lyophilized, powdered and stored for further characterization.

Drug (QRC) loading content of the QRC-FA-AgNPs
The QRC loading efficacy of the synthesized AgNPs was calculated using an indirect method of estimating the QRC content of the supernatant in the solution aer centrifugation at 10 000 rpm for 30 min. The drug concentration in the supernatant was measured under UV absorbance at 269 nm by using UV-visible spectroscopy (UV-2450, Shimadzu, Kyoto, Japan) and the percentage of loading of QRC in the synthesized AgNPs was estimated using the following formula.
In which w o is the weight of the QRC loaded on the FA-AgNPs and w is the weight of the synthesized FA-AgNPs.

Characterization of the QRC-FA-AgNPs
Following double dilution of the samples, a UV-2450 (Shimadzu, Kyoto, Japan) spectrophotometer was used to perform UV-vis-near-IR absorption spectroscopy. The possible composition of nanoparticles was determined using Fourier transform infrared (FTIR) spectroscopy (IR-Prestige 21; Shimadzu Corporation, Japan) in a scanning wave number ranging from 400-4000 cm À1 at a 2 cm À1 resolution following the KBr pellet method. X-ray diffraction (XRD) was carried out to analyze the nature of the synthesized NPs (Rigaku, Japan) while transmission electron microscopy (TEM, FEI-Tecnai G2 20 S-TWIN) was employed to establish the morphological features (size, shape, as well as structure) of the anisotropic nanoparticles which was followed by energy dispersive X-ray spectroscopy (EDX) and selected area electron diffraction (SAED) analysis. For TEM analysis a few drops of the synthesized nanoparticles were placed on copper grids (carbon coated) (Ted Pella, product code: 01800) and dried immediately in a vacuum desiccator. A Zeiss microscope (operating voltage; 200 kV) was used for recording the images and SAED was used to revalidate the crystallinity. In addition, dynamic light scattering (DLS) was performed for determining the average size distribution of the NPs, the polydispersity index and the zeta potential (surface charge) in a Nano ZS Zetasizer (Malvern Instruments Ltd., Malvern, UK) following appropriate dilution of the samples.
The stability of the QRC-FA-AgNPs was evaluated by incubating it in three biologically relevant media such as deionized water, cell culture medium (Dulbecco's modied Eagle's medium (DMEM), glucose and 10% fetal bovine serum (FBS)) and phosphate buffer (PBS) respectively, followed by daily determination of variations in the hydrodynamic diameter and polydispersity index (PDI) (Nano ZS Zetasizer; Malvern Instruments Ltd., Malvern, UK) of the NPs for a period of 14 d. 37 2.6. Analysis of in vitro QRC release from QRC-FA-AgNPs The dialysis method was implemented to estimate the release of QRC from the synthesized nanoparticles at 37 C in pH values of 7.4 and 5.5 respectively, corresponding to the physiological systemic environment. Briey, following 30 min stabilization of QRC-FA-AgNPs (containing drug equivalent 5 mg) suspended in PBS (1 ml), the suspension was placed in a dialysis bag (MW CO 2000 Da; Sigma-Aldrich). The pH of 10 ml of PBS was adjusted to 7.4 and 5.5 with subsequent submersion of the dialysis bag and incubation at 37 C in a water bath with constant stirring at 130 rpm respectively. At regular and predetermined time intervals 1 ml of PBS was withdrawn and immediately replaced with an equivalent amount of PBS maintained at similar conditions. The withdrawn samples were analyzed using a UV-2450 (Shimadzu, Kyoto, Japan) spectrophotometer at 269 nm. The experiments were performed thrice at individually selected pH values.

Cell line and cell culture
The human breast cancer (MDA-MB-231) cell lines were purchased from NCCS (National Centre for Cell Science) Pune, India and cultured in DMEM/F12 media supplemented with 10% FBS, streptomycin (100 U ml À1 ) and penicillin (100 mg ml À1 ). The cells were maintained in a humidied atmosphere of 5% CO 2 at 37 C.

In vitro cytotoxicity assessment and cellular uptake and internalization
Cytotoxicity was determined following an MTT assay. 38 Briey, following 24 h seeding of MDA-MB-231 cells at a predetermined concentration (10 4 cells per well) in 96 well plates, the cells were treated with varying concentrations of AgNPs, pure QRC, QRC-AgNPs and QRC-FA-AgNPs. Following 48 h of incubation further incubation of the cells was carried out for 2 h with 0.5 mg ml À1 of MTT solution at 37 C. Then, the medium consisting of MTT was aspirated with successive addition of DMSO (200 ml) in each well, ensuring solubilization of the formazan product thus formed. A microplate reader was used to measure the absorbance (Abs) at 570 nm and the comparative inhibition was calculated using: (Abs Control À Abs treatment )/Abs Control Â 100%.
The cellular uptake of the formulated AgNPs was evaluated in the MDA-MB-231 cells. 24 Briey, the cells were seeded at a density of 5 Â 10 4 cells in each well in 24 well plates, followed by 3 h incubation with uorescein isothiocyanate (FITC) labeled AgNP and QRC-FA-AgNPs. For FITC labelling, 100 mg fabricated AgNPs were added to 1 ml of FITC solution in absolute alcohol. Then, centrifugation of this mixture aer 4 h was consecutively followed by vacuum drying to obtain FITC-AgNP and FITC-QRC-FA-AgNP. A folic acid competition assay was performed by coincubating the QRC-FA-AgNPs with FA. Then, the cells were washed thrice with PBS and subjected to xation in paraformaldehyde solution (4%) with successive staining with Hoechst 33 342 solution (10 mg ml À1 ) in the dark for an hour. Finally, the uorescence of cells was analyzed using a confocal microscope (FLOWVIEW, Olympus).
2.9. Annexin V staining and ow cytometry to analyze apoptosis 38 MDA-MB-231 cells at a density of 2 Â 10 6 cells were subjected to treatment with AgNPs, pure QRC, QRC-AgNPs, QRC-FA-AgNPs and QRC-FA-AgNPs + NIR at predetermined concentrations for 24 h. Consequently, the cells were tryptinized and harvested along with PBS washing and then resuspended in binding buffer (100 ml) comprising CaCl 2 (1 mM), MgCl 2 (0.75 mM), KCl (4 mM), NaCl (140 mM) and 4-(2-hydroxyethyl)piperazine-1ethanesulfonic acid (HEPES) (10 mM) in double-distilled water (DDW). This was followed by 30 min staining of the suspended cells with annexin V-alexa uor 647 (BD Biosciences, San Diego, USA) at 4 C and the addition of propidium iodide (PI) to the stained cells aer proper washing. Immediately aer this, the cells were analyzed using a BD FACS calibur Flow Cytometry System and FCS Express soware (BD Bioscience, USA) was used for evaluating the results.

Analysis of in vitro photo-chemothermal efficacy 24
Determination of the photothermal effect on MDA-MB-231 cells was performed by incubating the cells with appropriate concentrations of AgNPs, QRC-AgNP and QRC-FA-AgNPs for 3 h followed by laser irradiation (800 nm, 1.5 W cm À2 , 5 min). Following an additional incubation for 3 h the cells were rst washed carefully with subsequent staining with calceinacetoxymethyl (AM) and PI as per the manufacturer's instructions (Cell health assay kit, Cell Signaling Technology). The chemo-photothermal effect was elucidated by incubating the cells with varying concentrations of AgNPs and QRC-FA-AgNPs for 3 h with subsequent exposure to NIR laser irradiation (800 nm, 1.5 W cm À2 ) for 5 min. Aer a successive 24 h of incubation of the cells, an MTT assay was performed to assess the cell viability.

Intracellular ROS levels 39
ROS production was estimated with the help of a dichlorodihydro-uorescein diacetate (DCFH-DA) analysis kit (Abcam) following the manufacturer's guidelines. Briey, MDA-MB-231 cells were primarily seeded in a 96 well plate. Treatment of cells with AgNPs, QRC-AgNPs, and QRC-FA-AgNPs was successively followed by removal of the media and further incubation of the cells with DCFH-DA (10 mM) and Hanks buffered salt solution (HBBS, 100 ml) for 30 min at 37 C. Measurement of the ROS was carried out with a spectrouorometer.

In vivo study design
The virgin female Sprague-Dawley (SD) rats (50-55 days old) required for the study were procured from the Central Animal Facility, Birla Institute of Technology (BIT) Mesra, Ranchi, Jharkhand, India (Reg. no. 1968/PO/Re/S/17/CPCSEA). The study was conducted in accordance with all the CPCSEA standards for the animal habitat, victuals and ethical considerations (Institutional Animals Ethics Committee, BIT Mesra, Ranchi, India) (1972/PH/BIT/61/18/1AEC).
Random distribution of the animals was performed meaning that six SD rats were included in each of the eight experimental groups. The complete in vivo study design of 118 d (ref. 40) is illustrated schematically in Fig. S1 (ESI). † The method for breast cancer induction in the SD rats, as well as the duration of the treatment with various drug formulations has been selected and performed following previously published studies. [41][42][43][44][45][46] Control rats in group I were administered with only the vehicle (olive oil (0.5 ml) on the day of induction and the treatment involved 1% sodium carboxymethyl cellulose (CDH, New Delhi, India)). Cancer was induced in SD rats belonging to group II (induced control) through the slightly modied air pouch technique 40 and they received 20 mg DMBA (suspended in 0.5 ml olive oil) on day 0. Aer 90 d of the tumor induction phase, the animals belonging to group III-VIII were treated with various drug formulations and/or laser for 28 d (ref. [41][42][43][44][45][46] and categorized as treatment groups. Group III animals received tail vein injections of AgNPs (40 mg kg À1 , body weight (b.w.)), group IV rats were given pure QRC (20 mg kg À1 , b.w., intravenously (i.v.)). Group V rats were exposed solely to NIR irradiation (800 nm, 1.5 W cm À2 for 5 min) for 14 d (every alternate day of the 28 d treatment protocol), and the group VI and VII animals received QRC-AgNPs and QRC-FA-AgNPs (equivalent to the amount of raw QRC administered, i.v.) respectively. Group VIII animals were treated for 28 d with QRC-FA-AgNPs (equivalent to the amount of raw QRC administered, i.v.) along with irradiation with continuous wave (c.w.) NIR laser (800 nm, 1.5 W cm À2 for 5 min) 24 h post-drug administration (i.e. 14 d, every alternate day for the 28 d treatment protocol). The entire experimental schedule included regular weekly monitoring of the b.w., as well as tumor location by palpation. Aer completion of the entire experimental protocol of 118 d, the animals were sacriced and perfused with subsequent isolation of the mammary tissues and other vital organs (liver, lungs, kidneys, heart, brain and spleen). While sections of isolated breast tissues were either preserved for performing uorescence terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) assay and eld emission scanning electron microscope (FESEM) analysis, the vital organs tissues were processed for histopathological evaluations.

FESEM analysis of mammary tissues 47
Mammary tissues isolated from all the animal groups were primarily xed in buffered formalin (10% v/v) and then subjected to dehydration with increasing strength of ethanol (70% v/v ethanol for 3 h followed by 80%, 90% and 100% v/v for 1 h respectively). Aer drying the tissue samples using a critical point dryer (CPD), they were mounted on stubs, sputter-coated with a gold layer and observed using FESEM (Sigma 300, ZEISS, Carl Zeiss).

In vivo apoptosis rate analysis through TUNEL assay 24
The tumor tissues isolated from all the groups of animals were primarily subjected to paraffin block embedding prior to a TUNEL assay to verify intra-tumoral late stage apoptosis. Click iT plus TUNEL assay kit (Thermo-Fisher) which employs Alexa Fluor 594 for in situ detection of apoptosis was used for staining the tissue sections (30 mm) in accordance with the manufacturer's instructions. Successively, the stained tissues slices were observed using a confocal microscope (FLOWVIEW, Olympus).

Histopathological analysis 47
Following sacrice, vital organs such as the heart, lungs, liver, kidneys, spleen and brain were isolated from the study animals and embedded in paraffin wax, a rotary microtome was used to cut ne tissue sections (6-8 mm) of these paraffin blocks comprising tissue. Then, these tissue sections were stained with haematoxylin and eosin dye following the standard experimental protocol.

Statistical analysis
Statistical comparisons of the data were determined by using one-way analysis of variance (ANOVA), followed by Bonferroni's multiple comparison tests with a similar number of samples. The level of signicance was measured at p < 0.05. All data were represented as mean AE standard error of the mean (SEM).

Results and discussion
One of the major problems with regards to chemotherapy is the dexterity of cancer cells to evade apoptosis, leading to therapy resistance. Thus, exploring key target molecules, as well as a stable delivery system, with a unique strategy to surpass resistance and restrict the side effects of the chemotherapeutic agents are the primary objectives of any ideal cancer therapy protocol. 48 However, various marketed drug delivery systems, such as those in trials are presently polyethylene glycol (PEG) based chemotherapeutic molecules. However, these are associated with issues relating to the balancing of the load with the holding capacity of the liposomal compartments with respect to the overall size of the nanoparticles, allowing uptake for intracellular localization. 49 Therefore, in this study we mainly aimed to synthesize FR-targeting plasmonic silver nanoparticles for QRC (QRC-FA-AgNPs) to investigate its cytotoxic and antiapoptotic efficiency in combination with photothermal therapy.

UV-visible-NIR absorption spectra analysis of QRC-FA-AgNPs
The AgNPs synthesized at an optimum hydrazine concentration of 40 mM (5 C and pH 5) manifested efficient plasmon tunable peaks from visible light to NIR. As shown in Fig. 1A, the rst surface plasmon resonance peak (SPR1) was observed at 408 nm, characteristic of the silver nanoparticles with a second distinctly prominent peak (SPR2) at 800 nm. Following folic acid conjugation along with QRC quenching (Fig. 1B), the nanoparticles were observed to be optically active with a marked red shi of SPR2 (800 / 861 nm) showing a strong NIR individual resonance while SPR1 (408 / 472 nm) became less prominent. However, a considerable red shi in both surface plasmon resonance peaks (SPRs) indicated FA and QRC conjugation on the surface of the AgNPs. 24,50 On the other hand, absorption peaks for QRC-FA-AgNPs at 269 and 350 nm represented the successful conjugation and quenching of QRC and FA respectively, as these peaks corroborated with the absorption spectra of the physical mixture of QRC + FA (Fig. 1C) that showed characteristic peaks at 265 and 350 nm corresponding to raw QRC and raw FA, respectively. 51,52

FT-IR analysis
The interactions and conjugation of FA and QRC was effectively studied using FT-IR spectroscopy.  52 These identifying peaks for both QRC and FA were found in the infrared (IR) spectral pattern of their physical mixture, with minor broadening or shi of peaks. Moreover, the synthesized nanoparticles exhibited similar spectral patterns to that of the raw ingredients with certain alterations in the vibrational frequencies. This indicates the successful conjugation of FA and QRC on the anisotropic silver nanoparticles. The shi in the spectral peaks was observed in many cases aer drug loading, 54 similar to our previous study, thus conrming the loading of the drug.

XRD analysis of the synthesized plasmonic nanoparticles
The XRD spectral pattern of the QRC loaded folate targeted nanoparticles was evaluated by comparison with that of the raw ingredients, as shown in Fig. 1E Fig. 1E(i). Fig. 1E(ii) reveals a closer view of both the FA and QRC conjugated nanocrystals, which shows some broadening of the peaks with little noise, which has previously been reported to be imparted owing to surface modication or coating of nanoparticles. 58  3.4. Hydrodynamic particle size and surface charge analysis along with morphological evaluation through TEM and EDX As shown in Fig. 2A, the particle size of the plasmonic nanoparticles (blank AgNPs) were found to be 19.68 AE 0.58, while aer FA conjugation, as well as QRC quenching, the particle size was found to be 47.58 AE 0.49 nm (Fig. 2B). High resolution (HR)-TEM revealed the shape of particles (blank and QRC-FA-AgNPs) to be distinctly pentagonal nanopyramids (Fig. 2E and F). These pentagonal QRC-FA-AgNPs demonstrated a robust plasmon tunability in the UV-vis-NIR region thus allowing absorption of light at higher wavelengths ($860 nm), which also facilitated deep tissue penetration. This phenomenon may result from plasmon coupling of adjoining nanoparticles as shown in previous studies. 59,60 The PDI values 0.246 AE 0.02 (AgNPs) and 0.265 AE 0.05 (QRC-FA-AgNPs) indicated a homogeneous and uniform dispersion of the nanoparticles (PDI < 3) which was also supported by the TEM images. Moreover, the zeta potential of the AgNPs (Fig. 2C) was found to be À24.0 mV, which may be imparted owing to the use of citrate as the capping agent. 25 Following the FA conjugation and QRC loading, the nanocarriers revealed a further negative surface charge of À33.5 mV (Fig. 2D) owing to folic acid functional groups and the negative OH groups of QRC. 25 Thus, while the overall particle size was well below 100 nm, the permitted feasibility for the cellular entry essential for drug delivery applications, 61 the negative zeta potential of QRC-FA-AgNPs resulted in repulsion between them, thus preventing occulation and allowing stability of the formulation 62 with proper dispersity.
On the other hand, the EDX (Fig. 2G) performed using the TEM validated synthesized nanoparticles comprised elemental silver (Ag), as a distinct absorption peak at around 3 KeV was observed, which is an identifying feature of metallic silver ascribed SPR. 61,63 The crystallinity and diffraction peaks of the synthesized AgNPs observed in the XRD spectra indexed to the (111), (200), (220) and (311) planes of the face centered cubic crystal AgNP structure were revalidated using SAED (Fig. 2H). 52,64

Drug loading and stability studies of QRC-FA-AgNPs
The loading efficiency of the synthesized silver nanoparticles was analyzed with a UV-vis spectrophotometer and theoretical drug loading capacity (DLC). With the theoretical DLC being set at 10 wt%, the synthesized AgNPs revealed a DLC of 8.13% that indicated successful loading of the drug.
The stability of the synthesized nanoparticles is a prerequisite parameter that should be determined. As observed from Fig. 3A(i), the particle size (hydrodynamic diameter), as well as the PDI (Fig. 3A(ii)) of the QRC-FA-AgNPs varied slightly within an acceptable limit over the incubation period of 14 d in all the three biologically relevant media of deionized water, cell culture medium and PBS, thus denoting the absence of particle aggregation.

Drug release study
Controlling the release pattern of the drug plays a vital role in improving the bioavailability of the fabricated QRC-FA-AgNPs. In this study, the release prole of QRC was evaluated from the QRC-FA-AgNPs at 37 C in two buffered aqueous solutions, one with a pH of 5.5 mimicking the tumor microenvironment or intracellular endosomes and one with a pH of 7.4 similar to the physiological pH. The result revealed that the acidic condition stimulated a more rapid release of QRC from the NPs compared to the physiological neutral system. Fig. 3B demonstrates the release of 80.1% and 66.8% of free QRC within 4 h of the experimental period in buffer solutions of pH 5.5 and 7.4 respectively. However, in contrast to this, the QRC released from the QRC-FA-AgNPs was found to be only 33.13% and 26.71% at the corresponding pH over a period of 4 h. Thus, it was evident from the release curve that, although the release of the pure drug attained saturation within 5 h, QRC from the NPs leaked smoothly in less than 30 h at a variable pH status denoting the capability of the QRC-FA-AgNPs to specically enhance the QRC delivery in cancer affected cells, correlating with earlier reports, which indicated that pH dependent drug release (i.e. increased efflux at acidic pH) signicantly augments drug delivery to cancer cells as the tumor microenvironment is well known to be acidic. 61 Hence, the engineered plasmonic nanoparticles, having a sufficient loading efficiency and QRC release prole, may be considered as an optimal nanocarrier system for consolidated curative action. 65

Improved targeted cellular internalization and in vitro cytotoxicity of QRC-FA-AgNPs
Folic acid fabricated nanocarriers have been diversely studied for therapeutic applications 66,67 as they provide a high selectivity to specic target folate receptors. The folate uptake mechanism is attributed to receptor mediated endocytosis, which involves initial internalization of a captured FA molecule by a FR followed by returning the FR to the cell surface for subsequent FA capture. A similar mechanism is applicable if FA is conjugated to nanoparticles. 24 Following internalization, the FA conjugates undergo dissociation in the acidic microenvironment of cancer cells and are released into cytosol. 68,69 In our study, the capability of specically targeting folate receptors (FRs) present on breast cancer cells was investigated by the cellular internalization of FITC tagged QRC-FA-AgNPs in MDA-MB-231 cells. As shown in Fig. 4A, following the incubation of cells with QRC-FA-AgNPs, the green uorescence intensity observed was much higher than that of cells incubated with bare AgNPs. Also, the overlay images of Hoechst + FITC + Phase demonstrated higher localization of the QRC-FA-AgNPs labelled with FITC in respect to AgNPs within the cells, indicating proper cellular internalization. This accentuated internalization may be a result of better recognition of FA functionalized AgNPs by FRs abundantly expressed on breast cancer cells, thus allowing adequate tumor specic accumulation of QRC-FA-AgNPs without harming the adjacent normal tissues. This observation was in accordance with previous studies involving diversied nanoscale carriers such as silver, 64 mesoporous silica, lignin based hollow nanoparticles, 70 Janus silver/silica based nanoplatforms 24 and so forth that revealed specic internalization and localization of nanoconjugates inside cellular organelles and the perinuclear region in each case. In order to conrm the FR targeted cellular uptake, co-incubation of cells with free folic acid was performed, which then indicated signicant reduction in the green uorescence of FA fabricated QRC-AgNPs, clearly suggesting that owing to competitive blockage of the FRs during FA pre-incubation resulted in diminished endocytosis of the nanoscopic-based cargoes.

Cytotoxic effects of QRC-FA-AgNPs as analyzed by MTT assay
As depicted in Fig. 4B, QRC-FA-AgNPs at different concentrations exhibited an escalating dose dependent toxicity in MDA-MB-231 cells. This elevated inhibitory rate of QRC-FA-AgNPs with respect to other formulations (blank AgNPs/QRC-AgNPs) or pure QRC on the viability of breast cancer cells may be explained as a consequence of the targeted cellular delivery and endocytosis of the nanocarriers via the FRs 71 making the cells more vulnerable to ROS and DNA damage. Consistent with our ndings, former studies involving QRC loaded nanoparticles 61,72 had shown notable cytotoxicity towards MDA-MB-231, compared to pure QRC.

Apoptosis inducing potential of QRC-FA-AgNPs in MDA-MB-231 cells
Reactive oxygen species are also one of the principle regulators of mitochondria governed intrinsic apoptosis. QRC alone has already been reported to participate in initiating apoptosis in various cancer cell lines (e.g. HT29, CD33 + cancer stem cells, MCF-7, BCG-823) 73-75 while its nano-formulations (using gold/ solid lipid nanoparticles) also effectively induce similar apoptotic mechanisms. 39,61 Additionally, AgNPs are known to bolster intrinsic apoptosis 65 attributed to ROS production, thereby activating proapoptotic factors in breast cancer or HepG2 cell lines. 58,76 Thus, to ensure that the chemotherapeutic potential of QRC-FA-AgNPs is mediated by apoptosis, uorescence activated cell sorting (FACS) analysis was performed (Fig. 5A). As demonstrated in Fig. 5D, the QRC-AgNPs showed signicantly high apoptosis with respect to pure QRC (Fig. 5C) or blank AgNPs (Fig. 5B). However, a remarkable upsurge in the early apoptosis indicated by AnnexinV+/PIÀ and late apoptosis as signied by annexin V+/PI+ breast cancer cells was observed following QRC-FA-AgNP treatment (Fig. 5E), which was further intensied by simultaneous laser exposure (Fig. 5F). Mediated by efficient folate targeting, the increased intracellular targeted uptake of QRC-FA-AgNPs resulted in a higher accumulation of the drug in cells and led to an enhanced apoptotic effect. Thus, plasmonic FA-AgNPs may act as an efficient carrier system for QRC for promoting the chemo-photothermal efficacy.

QRC-FA-AgNPs provoke prominent chemophotothermal therapy
Recent pioneering studies focus on designing plasmonic nanoplatforms capable of exhibiting a strong NIR resonance in which negligible excitation light absorption and scattering is exhibited by tissue chromophores (intrinsic) and water molecules enabling maximum tissue penetration of the radiation. Coupling of incident light with an oscillation frequency generated by conducting electrons of silver nanomaterials effectuate localized surface plasmon resonance (LSPR) that appears to be a strong absorbance band with a promising photo to heat transformation in tumor. 77 The prociency of hyperthermia reaches a maximum if the wavelength of the laser coincides with the absorption maxima of NIR. 78 In this study, the photothermal effect of QRC-FA-AgNPs was thus intuitively analyzed by calcein-AM and PI staining that allowed live and dead cells to be distinguished following NIR laser irradiation. Fig. 6A exhibited no signicant effect on the viability of cells when exposed solely to NIR irradiation. Cells incubated with AgNPs alone moderately increased the dead cell count aer NIR exposure because of their ability to selectively heat the local environment as they convert light absorbed LSPR into heat which was further potentiated aer QRC quenching. However, QRC-FA-AgNPs together with NIR showed the maximum dead cell count owing to higher internalization and accumulation of the nanocarriers inside cancer cells, facilitated by targeted endocytosis that improved the cytotoxicity of QRC along with production of sufficient local hyperthermia that enhanced the thermal sensitivity of breast cancer cells exposed to QRC. Similar results were observed in the cell viability study, in which the cells treated with QRC-FA-AgNPs exhibited maximum mortality under laser irradiation (Fig. 6A(ii)). Thus, the combined chemo-photothermal effect of the QRC-FA-AgNPs in the presence of NIR unveils an attractive strategy for improving the efficacy of QRC and balancing its dose.
Various researchers have reported promising cancer treatments (both in vitro and in vivo) induced by plasmonic nanoparticle triggered hyperthermia at the cellular, as well as the subcellular level. 79 It was evident from both our in vitro and successive in vivo assessments that upon exposure to NIR irradiation the QRC-FA-AgNPs provided an encouraging chemo-photothermal effect that improved the therapeutic efficacy in breast cancer. These ndings were successfully correlated with the chemo-photothermal effect as reported in the case of Janus silver/silica nanoplatforms for the treatment of liver cancer, 24 FA-conjugated silver nanotriangles for human ovarian cancer cells 25 and human non-small lung cancer cells, 73 graphene oxide mediated synergistic chemo-photothermal effect on murine mammary cancer cell line. 80

QRC-FA-AgNPs provokes intracellular ROS upsurge
The toxicological effect of the synthesized AgNPs is interlinked with their capability to provoke oxidative stress driven by intracellular ROS. 81 The tendency of AgNPs to localize in mitochondria collaborates with mitochondrial toxicity by increasing the ROS production. 76 In the current study, the ability of the different formulated AgNPs for inducing intracellular ROS production was investigated using the green uorescence stain DCFH-DA. As apparent from the results (Fig. 6B)  3.12. QRC-FA-AgNP remodeled DMBA-induced body weight changes and inhibited mammary tumorigenesis of breast cancer bearing animals The weekly changes in body weight (b.w.) recorded for the entire study protocol (118 d) are illustrated in Fig. 7A. A gradual and normal increase in b.w. was evident in the group I animals. Although in the initial 6-7 weeks of the proliferation stage, the b.w. of the cancer induced animals in group II was found to increase gradually, the latter stage was marked with subsequent reduction in b.w. that was highly signicant (p < 0.001) with reference to the group I animals at the end of the 118 d. Previous studies have also depicted a similar pattern of b.w. changes in cancer bearing animals, in which an accelerated decline in b.w. was observed from about 11 week following induction. 40,82 The anatomical variations, such as in b.w., reect the abnormalities and physiological anomalies. The loss in body mass may be an outcome of cancer cachexia. Some of the distinguished symptoms of cachexia are reduced b.w., early satiety, as well as anorexia, a non-specic type of anemia, and prominent muscle weakness along with changed host-metabolism or malabsorption. 83 Deterioration of b.w. is associated with loss of fat as well as muscle mass. The reduction in muscle mass may be attributed to the aggravated amount of alanine originating from degrading muscles and utilizing gluconeogenesis. 84 However, upon various drug treatments the b.w. of cancer induced animals has been found to increase in previous studies that denote their antineoplastic efficacy. [40][41][42][43] In our study, during the treatment phase (day 91 day 118), the animals receiving drug quenched silver nanoparticles (group VI, VII, VIII) also showed better improvements in the b.w. than those receiving blank AgNPs, raw QRC or NIR in comparison to the induced control group. QRC-FA-AgNPs (group VII) showed a highly signicant increase (p < 0.001) in the b.w. of the cancer induced animals in comparison to the induced control group, which may be due to the targeted delivery of QRC achieved by the silver nanoparticles. Moreover, animals receiving laser irradiation in association with the administered QRC-FA-AgNPs (group VIII) successfully normalized the altered b.w. resulting in an almost insignicant (p > 0.05) difference with the control group animals (group I). Thus, animals receiving plasmonic drug quenched folate targeted AgNPs demonstrated maximum protection, indicating the potentiation of QRC efficacy achieved by the silver nanoplatform owing to the combined chemophotothermal effect.
Administration of DMBA consistently results in progressive tumor development. Following induction with DMBA, cancer bearing SD rats attain promotion phase tumors aer 90 d. In the multistage tumorigenesis process the promotional stage is regarded as the reversible phase and is commonly studied for evaluation of the therapeutic effect of any drug. Thus, the post 90 d phase in the DMBA induced breast cancer model is suitable for any anticarcinogenic drug to cause prevention, reversal or even slowing down of the carcinogenic process. 46,85 In the current study, we also administered drugs and/or a laser during this stage (i.e. 91-118 d) of our 118 d experimental protocol and the effect of the combined chemo-photothermal therapy during this 28 d treatment period [41][42][43][44][45][46] is depicted in Fig. 7B. Following cancer induction, animals in group II developed large sized tumors which were signicantly reduced (p < 0.001) following QRC-FA-AgNP treatment (group VII) in comparison to pure QRC at the same dose as revealed by group IV animals (Fig. 7B). Moreover, when compared to group II animals, specic cancer cell ablation owing to hyperthermia achieved in rats belonging to group VIII by laser irradiation further improved the chemophotothermal efficacy of QRC-FA-AgNPs in group VIII, thereby assisting in the targeted damage of tumor cells. Therefore, at the end of the 28 d treatment phase, the combined chemophotothermal therapy revealed its potency to restrict and reduce tumor development.
Thus, the enhanced efficacy of QRC-FA-AgNPs to induce apoptosis was predominantly reected by the b.w. and tumor volume of the breast cancer bearing animals that were successfully ameliorated upon treatment. However, the chemotherapeutic potential of QRC-FA-AgNPs was improved when the animals were exposed to NIR radiation that conferred maximum protection attenuating the tumor volume and reinstating b.w.

QRC-FA-AgNPs remodeled normal architecture in breast cancer tissues as illustrated by FESEM
In order to analyze the morphological changes in breast tumor tissues before and aer treatment, FESEM was performed. As exhibited by Fig. 8B(ii), the breast cancer induced tissue showed membrane ruffles (MR) that indicate the invasiveness of the breast cancer and were also found to be densely covered with microvilli (MV) (Fig. 8B(ii)) that were absent in the control animal breast tissue (Fig. 8A). In addition, collagen bers originating in the surrounding matrix were observed to crosslink and form a dense network with strings of protrusions emerging from cancer cells. However, the dense accumulation of collagen bers (CL) including the network with the surrounding matrix was found to decrease remarkably following QRC-FA-AgNP therapy (Fig. 8D) in comparison to QRC treatment (Fig. 8C) and disappeared aer irradiation to NIR subsequent to QRC-FA-AgNP treatment (Fig. 8E). The MR were also minimized with restoration of normal architecture of the breast tissue, which further validated the chemo-photothermal therapy to be one of the most effective therapeutic strategies in treating breast cancer.
3.14. Hyperthermia enhanced QRC-FA-AgNPs invoked in vivo apoptosis and tumor growth inhibition A TUNEL assay was performed on the tumors excised from experimental animals and the rates of apoptosis were enumerated for further exploiting the mechanism associated with the combined antitumor activity. In Fig. 9A and B minimal uorescence signals were visible in the DMBA treated and DMBA + NIR receiving animals. While animals receiving blank AgNPs showed signs of initiation of apoptosis. Cancer induced animals treated with QRC-FA-AgNPs revealed signicantly advanced apoptosis. As manifested from our previous in vitro results, treatment with QRC-FA-AgNPs + NIR conferred a maximum rate of in vivo tumor apoptosis in comparison with the other groups of animals. Thus, the extent of intra-tumoral Fig. 10 Histopathological studies (H&E) staining of vital organs (lungs, liver, kidneys, heart, brain, and spleen) tissues to determine biocompatibility isolated from animals belonging to different experimental groups (400Â) in which group I: control; group IIinduced control (DMBA, 20 mg); group VII -DMBA (20 mg) + QRC-FA-AgNP (20 mg kg À1 , b.w.), group VIII -DMBA (20 mg) + QRC-FA-AgNP (20 mg kg À1 , b.w.) + c.w. NIR (800 nm, 1.5 W cm À2 , 5 min). apoptosis and TUNEL positive cells, respectively, clearly suggested the successful and improved tumor growth inhibition by the photothermal effect supplemented chemotherapeutic potency of QRC-FA-AgNPs in the presence of NIR. 24

Biocompatibility of QRC-FA-AgNPs indicated by histopathological evaluation
Haematoxylin and eosin staining illustrated non-signicant pathological alterations in the isolated vital organ tissues of the heart, liver, lungs, kidneys, brain and spleen indicating the absence of inammation, lesions or tissue damage (Fig. 10). Animals belonging to groups VII and VIII showed an improved biocompatibility, organ specicity and targeted drug delivery owing to FA fabrication of the QRC loaded AgNPs when compared with animals receiving bare AgNPs and raw QRC, which showed subtle signs of acute inammation in the vital organs, signifying signs of side effects that may be associated with free drug administration. Overall, QRC-FA-AgNPs serves as a biocompatible nano-delivery cargo for QRC, the efficacy of which can be improved when exposed to NIR irradiation mediated by photothermal therapy.

Conclusion
In contrast to the typical (spherical) AgNPs with a restrained plasmonic resonance at $450-500 nm, a novel folate receptor targeted silver based QRC nano delivery system with characteristic plasmon tunability in the NIR region ($860 nm) was modelled in the current study for combined chemophotothermal therapy of breast cancer. To surmount the inadequate water solubility of QRC and improve the biocompatibility, we designed favorable NIR triggered nano-cargoes that showed efficient internalization in breast cancer cells through folate receptors. Predominantly, the photothermal effect induced by the silver nanocarrier supplemented the antitumor efficacy of QRC by inducing hyperthermia, thus selectively killing the cancer cells and resulting in apoptosis. Moreover, the QRC loaded AgNPs exhibited a synergistic antitumor effect in both in vitro and in vivo investigations (Fig. 11). Conclusively, the functionally upgraded plasmonic QRC-FA-AgNPs proved to be more potent than free QRC and could be used as a sequential strategy for combined therapy for breast cancer.

Conflicts of interest
There are no conicts to declare.

Financial support and sponsorship
This research was fully funded by AICTE under the project grant no. 8-69/RIFD/RPS/POLICY-1/2016-17.