Cytokinesis-Block Micronucleus Assay Using Human Lymphocytes as a Sensitive Tool for Cytotoxicity/Genotoxicity Evaluation of AgNPs

Silver nanoparticles (AgNPs) are the most used nanomaterials worldwide due to their excellent antibacterial, antiviral, and antitumor activities, among others. However, there is scarce information regarding their genotoxic potential measured using human peripheral blood lymphocytes. In this work, we present the cytotoxic and genotoxic behavior of two commercially available poly(vinylpyrrolidone)-coated silver nanoparticle (PVP–AgNPs) formulations that can be identified as noncytotoxic and nongenotoxic by just evaluating micronuclei (MNi) induction and the mitotic index, but present enormous differences when other parameters such as cytostasis, apoptosis, necrosis, and nuclear damage (nuclear buds (NBUDs) and nucleoplasmic bridges (NPBs)) are analyzed. The results show that Argovit (35 nm PVP–AgNPs) and nanoComposix (50 nm PVP–AgNPs), at concentrations from 0.012 to 12 μg/mL, produce no changes in the nuclear division index (NDI) or micronuclei (MNi) frequency compared with the values found on control cultures of human blood peripheral lymphocytes from a healthy donor. Still, 50 nm PVP–AgNPs significantly decrease the replication index and significantly increase cytostasis, apoptosis, necrosis, and the frequencies of nuclear buds (NBUDs) and nucleoplasmic bridges (NPBs). These results provide evidence that the cytokinesis-block micronucleus (CBMN) assay using human lymphocytes and evaluating the eight parameters provided by the technique is a sensitive, fast, accurate, and inexpensive detection tool to support or discard AgNPs or other nanomaterials, which is worthwhile for continued testing of their effectiveness and toxicity for biomedical applications. In addition, it provides very important information about the role played by the [coating agent]/[metal] ratio in the design of nanomaterials that could reduce adverse effects as much as possible while retaining their therapeutic capabilities.


■ INTRODUCTION
Silver nanoparticles (AgNPs) have been widely used in consumer and industrial products and recently in biomedicine, mainly because they exhibit beneficial properties by acting as broad-spectrum antimicrobials, and anti-inflammatory, antiviral, and antitumor agents, among other features. 1−4 Due to their extensive uses, several concerns have been raised as to whether exposure to AgNPs can produce cytotoxic and genotoxic effects in humans. However, it is difficult to objectively compare the different studies because they include a wide variety of factors such as size, shape, coating, or stabilizers in addition to the diverse biological test models and the biomarkers used to identify toxicity.
Different articles report the cytotoxic and genotoxic effects of AgNPs; 5−13 however, the lack of a systematic model to identify their genotoxic potential makes it hard to make decisions regarding the safety of nanomaterials, mostly when standard models are used, like the Ames test, which does not provide reliable results. 14 In this sense, cytokinesis-block micronucleus (CBMN) assay could fulfill this requirement; this technique, recently endorsed by the OECD, 15 is considered one of the most robust methods for assessing cytotoxicity and genotoxicity since it provides nine biomarkers. For cytotoxicity, the cytokinesis-block proliferation index (CBPI) or replication index (RI) and its associated cytostasis percentage (% Cyt), apoptosis, and necrosis are the biomarkers. The former shows the proliferative capacity of human lymphocytes under the experimental conditions tested. On the other hand, apoptosis and necrosis indicate the number of cells dying through regulated processes or linked to severe damage events that exceed the ability of the cell to repair itself, respectively. 16 In the case of genotoxicity, frequencies of micronuclei (MNi), nuclear buds (NBUDs), and nucleoplasmic bridges (NPBs) are the biomarkers. MNi, being the product of clastogenic and/or aneugenic processes, are widely described in the literature. 17,18 NBUDs have been described as amplified reparation DNA fragments produced in response to damage elicited by reactive oxygen species (ROS) and reactive nitrogen species (RNS), while NPBs are the result of (1) cellular repair processes, (2) poorly matched chromosomes that arise from damage in the mitotic spindle related to inhibitors in the synthesis and repair of DNA, and (3) general chemical agents that break the phosphodiester structure of DNA ( Figure  1). 18,19 The use of the CBMN assay allowed the authors to emphasize the role of the size, shape, and type of the stabilizer used as a coating agent in the cytotoxic and genotoxic effects observed. To our knowledge, the CBMN assay is nevertheless underused and just a few authors have evaluated the effects of AgNPs with it. 20−22 Furthermore, in practically all of the cases, they find that the silver nanoparticles assessed are cytotoxic and produce genotoxic damage at low concentrations. 23−26 Overall, it has been observed that AgNP formulations evaluated on human lymphocytes produce genotoxic damage, with an intensified effect produced by the uncoated nanoparticles. 24,26−29 Butler and co-workers found an increase in the number of cells with micronuclei with size and concentration dependencies; the smaller the size of the nanoparticle, the higher the observed percentage of cells with micronuclei and the lower the concentration of nanoparticles needed to produce the effect. 28 Even so, the sensitivity of preserved lines is lower compared with primary cultures as shown by Greulich, 30 a factor that definitely affects the observed genotoxic effect. This difference in cellular sensitivity is one of the main reasons to strongly recommend the use of primary cultures as the first step to determine the cytotoxic and genotoxic damage produced by nanomaterials.
On the other hand, in the last few years, our research group studied an AgNP formulation that has been fully characterized using different physicochemical techniques and applied in several research areas with outstanding results. 31−40 In Russia, this formulation was approved for use in humans as a nutritional supplement, in cosmetics, and recently in hemostatic sponges in surgery. 32 Its application led to remission of symptoms and re-epithelialization of tissues in the treatment of human otitis. 33 Besides, topical application proved to be useful for the rapid healing of patients with chronic diabetic foot ulcers. 34 This formulation produces a remarkable antiproliferative effect on solid human tumor cell lines, 31 which could be triggered by ROS overproduction. Moreover, we recently found that it reduces tumor growth and increases the lifespan of mice with melanoma more than twofold compared with cisplatin. 41 In veterinary applications, results showed their antiviral effectiveness in treating infectious rhinotracheitis and bovine viral diarrhea, 35 Rift Valley virus in mice, 36 canine distemper, 37 and the white spot syndrome virus (WSSV) in shrimp. 38,42 Our multidisciplinary research group has also published that concentrations of 25 and 50 mg/L of this AgNP formulation are useful as an antibacterial agent for sanitizing the culture media used for micropropagation of plants. 39 Furthermore, at these particular concentrations, it elicits a hormetic effect on high-value commercial crops such as sugar cane and vanilla. 39,40 All of these processes occur without cytotoxic or genotoxic damage to the plant after 6 weeks of exposure. 43 Due to these findings, our group is focusing on new biomedical applications for this AgNP formulation. Therefore, it is crucial to assess the biocompatibility of this nanomaterial, particularly its genotoxic potential in primary cultures.
This work aims to evaluate the cytotoxic and genotoxic effects of the commercially available AgNP formulation Argovit on peripheral blood human lymphocytes of a healthy donor. It will be done following the CBMN model proposed by the OECD through the evaluation of nine biomarkers. 44 Then, the results will be compared with the findings reported for other AgNPs and other chemicals to evaluate whether the CBMN assay can be proposed as a low-cost technique, reproducible and effective for a first screening of cytotoxic and genotoxic profiles of nanomaterials.

■ RESULTS
The measurement of CBPI indicates the cytotoxic effects due to the exposure to different concentrations of the chemical agents assessed. As expected, our results showed that sodium arsenite produces a major decrease in the CBPI (1.216 ± 0.156) compared with the negative control (1.500 ± 0.002). A similar effect was observed for poly(vinylpyrrolidone) (PVP) and 50 nm PVP−AgNPs, with CBPI values in the range of 1.21−1.26 for the former and 1.18−1.36 for the latter. Interestingly, no difference was observed between 35 nm PVP−AgNPs and the control group (Figure 2a). The differences in cellular toxicity elicited by each agent are most evident on analyzing the replication index (RI) (Figure 2b), where 35 nm PVP−AgNPs do not show differences compared with the control; meanwhile, the other evaluated agents decrease it by more than a half, like sodium arsenite, with values of 21.6% and the lowest concentration assayed of 50 nm PVP−AgNPs (0.012 μg/mL) that exhibited 36.5%.
The cytostasis induced by each agent clearly shows that sodium arsenite significantly affects the cellular division, the same as PVP at both concentrations evaluated, with more than 50% cytostasis. However, the most relevant result for us was the difference found between both AgNP formulations: 50 nm   figure) was performed; the bars represent the mean ± standard deviation of three independent experiments. * Indicates significant differences (p ≤ 0.05), ** indicates very significant differences (p ≤ 0.01), and *** indicates highly significant differences (p ≤ 0.001) compared with the negative control (lymphocytes without treatment), while § indicates significant differences (p ≤ 0.05) compared with the positive control (sodium arsenite).
On the basis of apoptosis and necrosis data, we confirm the differences regarding cytotoxicity elicited by both AgNP formulations. While 35 nm PVP−AgNPs do not show differences from the control at any of the concentrations assessed, all of the concentrations of 50 nm PVP−AgNPs showed higher apoptosis induction than those of the control and even sodium arsenite (Figure 2e), although the concentration of 0.12 μg/mL of 50 nm PVP−AgNPs was the only one that showed a statistically significant difference. Apoptosis on lymphocytes exposed to sodium arsenite increased to 8.4%, while those exposed to PVP at concentrations of 0.018 and 188 μg/mL showed 7.0 and 7.6%, respectively. Even though none of them present a significant difference in the apoptosis induction compared with the control, the average rate of apoptotic cells found in healthy donors is 1%. 17 Under our experimental conditions, the apoptosis was 1.09% ( Figure 2e).
On the other hand, the necrosis induction showed impressive and opposite results for 35 and 50 nm PVP− AgNPs. The 50 nm PVP−AgNPs are the most cytotoxic agents evaluated in this work, producing, at all concentrations assessed, from 43.5 to 68.0% necrotic cells, which is more than double the rate of necrosis produced by sodium arsenite. In contrast, none of the 35 nm PVP−AgNP concentrations assayed showed a significant difference compared with the control, despite the fact that the coating agent alone induced an increase of necrosis by itself ( Figure 2f). The vehicle control (PVP 12.6 ± 2.7 kDa) at both concentrations assessed showed induction of necrosis of 32.0 and 45.7% for the concentrations of 0.0188 and 188 μg/mL, respectively. The necrosis found for PVP was even higher than that produced by sodium arsenite (Figure 2f). Necrosis observed in the lymphocytes exposed to sodium arsenite was 15.6 ± 0.17%, almost double the upper bound of 9% reported by Fenech in healthy donors. 17 Under our conditions, no necrosis was identified for untreated lymphocytes (Figure 2f).
Cytotoxic results are consistent with the registered genotoxicity. Even though micronuclei frequency differences compared with the control were observed for none of the substances at any of the concentrations assessed, the nuclear damage was manifested as NBUDs and NPBs. All compounds, regardless of the concentration used, showed a micronuclei frequency within the established range for healthy donors, 17 marked as dotted lines in Figure 3a.
Sodium arsenite and PVP do not produce an increase in micronuclei frequency compared with the negative control but significantly increase the rate of NBUDs and NPBs ( Figure 3). Micronuclei absence could be related to the high cytotoxicity, probably because nuclear damage increases as the cytotoxic damage increases. Sodium arsenite that presents the most significant decrease in RI or NDI ( Figure 2) showed one of the highest frequencies registered of NBUDs and NPBs of 21.87 ± 0.79 and 83.42 ± 0.76 for every 1000 BN cells, respectively, only exceeded by the effect produced by the 50 nm PVP− AgNPs, whose behavior will be discussed later (Figure 3b,c).
PVP that also induces apoptosis and necrosis greater than that of the control at both concentrations assayed showed frequencies of NBUDs and NPBs of 11.06−14.74 and 27.50− 36.96, respectively. Similar results to those described for sodium arsenite and PVP were found for 50 nm PVP−AgNPs. These nanoparticles produce the highest cytotoxic and genotoxic damage among all of the compounds evaluated (Figures 2 and 3). The highest values of apoptosis and necrosis were produced by this AgNP formulation ( Figure 2). In the same way, NBUDs and NPBs showed the highest frequencies with 13−36 NBUDs and 33− 96 NPBs for every 1000 BN cells ( Figure 3).
In contrast, the 35 nm PVP−AgNPs produce neither cytotoxic nor genotoxic effects. None of the concentrations assessed provide statistically significant differences for any of ■ DISCUSSION CBMN Assay Impact. The evaluation of cytotoxic and genotoxic effects of nanomaterials represents a contemporary issue due to their widespread presence around the world, especially for silver nanoparticles with potential biomedical applications. Furthermore, the model used for evaluating the toxicity of AgNPs remains one of the greatest challenges. In this regard, the use of reliable and comparable data from reproducible and accurate methodologies is essential to facilitate decision-making on safe work with nanomaterials. Particularly, the CBMN assay is a robust technique that allows the evaluation of the nine biomarkers simultaneously, providing remarkable results with primary cultures, especially with human peripheral blood lymphocytes (HPBLs), which, for a long time, have been identified as extremely sensitive indicators of genotoxic damage. 45 However, to our knowledge, this technique has been underused for the cytotoxicity and genotoxicity evaluation of AgNPs. 26,46−49 The results presented in Figures 2 and 3 demonstrate that the CBMN assay is a useful and reproducible technique as a first approach to determine the cytotoxic and genotoxic effects of silver nanoparticles but also show the mandatory need to consider all nine biomarkers provided by the model to establish them.
It is very important to note that NDI values for all agents assessed are within the range reported by Fenech for a population of healthy donors, 1.3−2.2, 17 with the exception of sodium arsenite, which shows a decrease in value to 0.7. Therefore, just analyzing NDI values could lead to the erroneous conclusion that both AgNP formulations present the same response of no cytotoxic damage.
When searching the literature for the response of other AgNP formulations regarding the evaluation of CBPI, RI, or cytostasis parameters, we could not find other studies. Even though the data are the same, the way in which multinucleated cell contribution is considered allows us to observe differences between the values of CBPI and NDI (eqs 1 and 4), although the values of the biomarkers, index of replication and percentage of cytostasis, enable us to better differentiate the cytotoxic effects of the different agents evaluated.
Furthermore, none of the reference substances recommended by the OECD on Guideline 487 "In Vitro Mammalian Cell Micronucleus Test" 15 produce cytotoxic and genotoxic damage through ROS overproduction; instead, they produce cell damage by direct interaction with DNA, inhibiting DNA synthesis, or interacting with the microtubules and interfering with their functions.
Arsenic Cytotoxic Effect on HPBLs. The arsenic cytotoxic and genotoxic effects on HPBLs have been widely described in the literature. 50,51 Half-inhibitory concentration (IC 50 ) of sodium arsenite on peripheral mononuclear (MONO) cells has been reported as time-dependent, with values higher than 10 μM (1.29 μg/mL) after 24 h of exposure and 5 μM (0.64 μg/mL) after 48 and 72 h. 52 Cytotoxicity of sodium arsenite could be triggered by reactive nitrogen species (RNS) and reactive oxygen species (ROS) in a mitochondrialdependent pathway, which could overwhelm the antioxidant system of the cells eliciting the apoptotic and necrotic processes. 51,53 Due to the lack of data related to CBPI, RI, or percentage of cytostasis for sodium arsenite in human lymphocytes, we use the NDI values for comparative purposes. The NDI value found in the present work for HPBL exposure to 1 μM sodium arsenite agrees with that reported by Colognato 54 (Figure 2).
The production of ROS could explain the increase of apoptosis and necrosis events promoted by sodium arsenite on HPBLs found in this work (Figure 2e,f). Apoptosis is 2 times higher than that registered for the control, while necrosis is 4 times higher. It is important to recall that lymphocytes were exposed only for 20 h to sodium arsenite due to its high toxicity. This fact also explains the lack of differences found in the micronuclei frequency compared with the control.
However, this does not mean that nuclear damage promoted by sodium arsenite does not exist. We could not expose the arsenite at the same time as AgNPs because it leads to complete lymphocyte decomposition. Measurement of only the micronucleus for genotoxicity evaluation is not enough; frequencies of nuclear buds and nucleoplasmic bridges (NPBs) should also be measured.
We observed nuclear damage by the substantial increase of BUDs and especially of PBDs. MNi can originate from fragmented chromosome material when NPBs are formed, stretched, and broken during telophase. NPBs arising from misrepair of DNA breaks are also likely to be associated with MNi originating from the acentric fragment generated during misrepair. Therefore, the presence of NPBs but no MNi could be related to the repair capacity of the cell within a specific time frame. 18 A similar effect to that described for sodium arsenite was found with hydrogen peroxide. 16 Cytotoxic and Genotoxic Effects of PVP. Contrary to sodium arsenite, scarce information about the cytotoxic effects of PVP directly on HPBLs can be found. The lack of information could be due to the extremely low toxicity generally reported for this polymer. The half-lethal dose (LD 50 ) commonly reported for PVP is higher than 100 g/kg administered orally and 10 g/kg intravenously or intraperitoneally. 55 Unfortunately, in many of the reports, the molecular weight of the employed polymer is not indicated, 56 although PVP toxicity relies upon it. 57 Just analyzing the NDI values, it seems reasonable that PVP, at both concentrations employed here, does not affect cell division of HPBLs exposed to it (Figure 2a). However, both PVP concentrations assayed present similar CBPI, RI, cytostasis, and apoptosis and even a higher necrosis induction than that of sodium arsenite ( Figure  2b,c). Thus, PVP used in this work (with a molecular weight of 12.6 ± 2.7 kDa) is not entirely innocuous as claimed in the literature, at least for the isolated HPBL cultures. The obtained result is a clear example that NDI does not provide enough information about the cytotoxicity of the substances. Surprisingly for us, the same can be said for the genotoxicity elicited by PVP recorded as the MNi frequency. Although the micronuclei frequency is less than the value found in healthy donors, the rate of NBUDs and NPBs is always higher than the reference value and, for both concentrations of PVP assessed, those biomarkers are several times higher than the values found for the control (Figure 3).
AgNPs Cytotoxicity and Genotoxicity. Now, for different AgNP formulations, no changes in NDI and MNi compared with the negative control were observed ( Figure  2). However, analyzing the data for CBPI, RI, cytostasis, apoptosis, necrosis, NBUDs, and NPBs, a different trend was found. While 35 nm PVP−AgNPs produce no changes in CBPI, RI, cytostasis, apoptosis, or necrosis compared with the control, 50 nm PVP−AgNPs produce the highest amount of apoptosis, necrosis, NBUDs, and NPBs of all assayed compounds, producing almost the same frequency of NPBs and twice the necrosis and NBUDs than that induced by sodium arsenite.
As previously reported, 18 NBUDs can be interpreted as DNA-damage repair attempts by amplifying the genetic material that is removed during the S phase of the cell cycle. 58,59 On the other hand, the NPBs originate during anaphase and are formed when the centromeres of the dicentric chromosomes extend to the opposite poles of the cell during mitosis, resulting in a union between both new nuclei formed. 18,60,61 Reasons for Differences in Cyto-and Genotoxicity of Two Types of AgNPs. Despite the similarity in the size, shape, ζ potential, and coating of 35 and 50 nm PVP−AgNPs, we found enormous differences in the response of human peripheral blood lymphocytes to these nanoparticles. These results reflect nucleic material damage that could be caused by the formation of amplified telomeric regions of DNA that can eventually be separated from the nucleus, creating chromosomal aberrations such as NBUDs and NPBs, which ultimately could be manifested as MNi. 18,61 Furthermore, the above results are opposite to the findings described in other studies, where the smaller the AgNPs, the greater the cytotoxic and genotoxic damage; 24,26−28 thus, other factors must contribute to the differential response observed.
Several studies describe that the use of PVP as a coating agent decreases the cytotoxic and genotoxic effects of AgNPs for different cell types compared with bare nanoparticles or other coating agents such as citrate. 62 Vecchio and co-workers found that PVP−AgNPs decrease the genotoxic damage on human lymphocytes compared with citrate−AgNPs despite the size. Even for the smaller nanoparticles, the genotoxic effects were milder when PVP was used as a coating agent compared with AgNPs of similar size stabilized with citrate. 26 Even then, the changes found by Vecchio and co-workers are not as remarkable as those found in this work, but we must consider that they only analyzed the difference in the frequency of micronuclei without consideration of any other biomarker.
Contribution of Silver Ion Toxicity. The most important changes regarding cytotoxicity and genotoxicity could be expected for less stable AgNPs if we consider that IC 50 for silver ions on HPBLs is 1.1 μM (0.1 μg/mL Ag + ). Several reports have shown that these less stable AgNP formulations, such as uncoated 3−7 nm AgNPs studied by Joksićand coworkers, 24 produced a significant increase of cytotoxic and genotoxic damage with size and concentration dependencies on human lymphocytes. Despite the fact that the higher the nanoparticle concentration, the lower the NDI and the higher the MNi frequency, 24 no generalization in the biological response can be made because, in the same work, the authors found that 2 nm uncoated AgNPs promote cell proliferation, which is closely related to a definite increase of insulin-like growth factor-1. 24 A further interesting example of the coating agent effect on human lymphocytes is provided by Ivask and co-workers, who found that citrate−AgNPs (IC 50 = 12.5 μg/mL) are less cytotoxic than the more stable polyethyleneimine−AgNPs (IC 50 = 5.25 μg/mL), both nanoparticles with practically the same size (18 nm). 27 The cytotoxicity of the former is attributed to the Ag + ion release due to its lower stability, while for the latter, the cytotoxicity is attributed to the whole nanoparticle. The dissolution rate shows that the released ions by polyethyleneimine−AgNPs cannot generate the cytotoxic damage observed. Interestingly, both 18 nm AgNPs promote the appearance of apoptosis markers in 10% of the cells at all evaluated concentrations, while a concentration-dependent behavior was observed for necrosis, reaching values up to 50% of the cell population for the higher concentrations employed. 27 In the present work, a fairly comparable response on the necrosis induction was found on lymphocytes exposed to 50 nm PVP−AgNPs, reaching up to 45%, but just the opposite response for 35 nm PVP−AgNPs. This allows us to propose that silver ion release from 35 nm PVP−AgNPs could not be much faster than, or in comparable amounts to, silver ions released by 50 nm PVP−AgNPs.
Ag/PVP Ratio Impact on Toxicity. To our knowledge, despite the differences in the size and coating agents employed, none of the revised results have shown sharp differences in cytotoxicity and genotoxicity such as those found here. We consider that the differences of HPBL responses could rely upon the Ag/PVP ratio used to fabricate these PVP−AgNP formulations. According to the corresponding suppliers, Ag/ PVP w/w ratios for 35 and 50 nm PVP−AgNP formulations are 6/94 and 36/64, respectively. The small concentration of Ag and the high concentration of the PVP stabilizer in the 35 nm PVP−AgNP formulation prevent the release of an essential amount of silver ions that could produce cytotoxic and genotoxic damage. Our research group has determined that after 24 h of incubation in Roswell Park Memorial Institute (RPMI) medium, the silver ion released from 10 μg/mL solution of 35 nm PVP−AgNP formulation was 0.024 μg/mL, quantified by inductively coupled plasma optical emission spectrometry (ICP-OES), 63 which is 1000-fold lower than the dietary silver intake in Canadian women (27 μg/day), and at least 16-fold the human use level for the general population (0.4−30 μg/day). 64 Therefore, the biological response observed for this formulation cannot be attributable even to the silver ion released or to the coating agent.
Despite the fact that the coating agent elicits its own cytotoxic and genotoxic damage, the response is still different from that observed for the whole nanoparticle. The results suggest that the lack of cytotoxic response for 35 nm PVP− AgNPs can be attributable to the whole nanoparticle, which has a very stable formulation achieved by the high amount of PVP employed. In contrast, the use of a lower amount of coating agent could produce a less stable formulation, from which the released silver ions increase the cytotoxic and genotoxic damage like that observed for 50 nm PVP−AgNPs.
The higher stability due to the significant amount of PVP in the 35 nm PVP−AgNP formulation also could help explain the remarkable antitumor activity of this formulation observed in mice with melanoma, which decreases more than twice the tumor growth compared with cisplatin, but without apparent genotoxic damage observed for the mice. 41 Impact of the Cellular Type Exposed to AgNPs. The identification of the factors that provoke the biological response of each formulation of AgNPs is critical. The differential cytotoxicity elicited by AgNPs is related not only to their physicochemical characteristics but also to the cellular type to which they are exposed. Greulich and co-workers 30 show that concentrations of 30 μg/mL (regarding total silver) of specific PVP−AgNP formulations do not decrease the cellular viability of T-lymphocytes isolated from human peripheral blood, while concentrations starting from 20 μg/ mL of the same PVP−AgNPs show a concentration-dependent behavior in monocyte viability of the same blood sample. A commercially available 20−30 nm PVP−AgNP formulation also presents differential toxicity between tumorigenic and nontumorigenic cells (PVP, 0.2 wt % SkySpring Nanomaterials, Inc., Houston, TX). This formulation showed higher growth inhibition potency on triple-negative breast cancer cells MDA-MB-231, BT-549, and SUM-159 than those in the nontumorigenic cell lines MCF-10A, human mammary epithelial cells (HMECs) (184B5), and in post-stasis HMECs. 65 Concentrations of 35 nm PVP−AgNPs Effective as Antiproliferative, Antitumor, and Antiviral Agents.
Comparing the results obtained in this work with others found by our group about the viability of human tumor cell lines exposed to the same 35 nm PVP−AgNP formulation, we found an excellent selectivity regarding cytotoxic effects. We found that IC 50  In all of the tumor cells, apoptosis is the main activated pathway, probably triggered by ROS overproduction. No evidence of DNA damage or genotoxicity was observed on these cells. 31 It is remarkable that a concentration of 12 μg/mL that represents 3.5−6.6 times higher concentration than that used to eliminate the halfpopulation of tumor cell cultures does not show any sign of cytotoxicity and much less of genotoxicity on human peripheral blood lymphocytes (Figures 2 and 3). The abovedescribed selective cytotoxic effect provides an opportunity to continue the AgNP preclinical protocols for cancer treatment.
Other uses of this AgNP formulation, by which humans can be in touch with it, include its use as an antiviral agent against the white spot syndrome virus (WSSV), 42 treatment of canine distemper, 37 or application as sanitizing and hormetic agents on plant micropropagation cultures. 39,40 As far as we know, this is the first report on the effect of silver nanoparticle formulations using the nine biomarkers provided by the blocking cytokinesis (CBMN) assay in human lymphocyte cells from healthy donors. Furthermore, this is the first time that enormous differences regarding the cytotoxic and genotoxic effects of AgNP formulations that are quite similar in size, shape, ζ potential, and coating agent were reported.

■ CONCLUSIONS
This work allows us to conclude that the CBMN assay using human blood peripheral lymphocytes with the analysis of the nine biomarkers provided by the technique is a useful, reproducible, and low-cost option as a first approach to the cytotoxicity and genotoxicity of nanomaterials.
The 35 nm PVP−AgNP formulation (Argovit) presents no evidence of cytotoxic or genotoxic damage to the human peripheral blood lymphocytes for the studied concentrations, in contrast to the very similar AgNP formulation (50 nm PVP−AgNPs, NanoComposix). Due to this comparison, the fundamental role played by the [coating agent]/[metal] ratio in the biological response of the manufactured nanoparticles and their toxicity was suggested, providing valuable information for the design and production of safe nanomaterials.
The use of the nine biomarkers instead of the two or three commonly reported in the literature provides impressive differences with respect to the cytotoxic and genotoxic potential of the nanomaterials evaluated. As was shown in this work, this technique is a very sensitive and powerful tool that allows researchers to establish the cytotoxic and genotoxic differences of two AgNP formulations with very similar physicochemical properties and that did not seem harmful when evaluating the common biomarkers' nuclear division index and the frequency of micronuclei.
The obtained results also revealed the cytotoxic selectivity of the AgNP formulation Argovit. The concentrations used to produce remarkable growth inhibition in several systems such as tumor cells, virus, and pathogen bacteria are the same ones that produce scarce cytotoxic and genotoxic damage on primary human culture (lymphocytes). This selectivity can be applied to generate therapeutic alternatives not only for cancer, virus, or bacteria but also for immunologic, neurodegenerative, or chronic−degenerative diseases.
These results will contribute to providing a complete picture regarding the safe production and use of manufactured AgNP formulations in the different application areas already explored and the new ones to come.

■ MATERIALS AND METHODS
Silver Nanoparticles (AgNPs). We selected the commercially available AgNP formulation Argovit because it has been used in multiple application areas and has certificates for use in veterinary and human applications. 33,37 Silver nanoparticles were donated by Dr. Vasily Burmistrov of the Vector-Vita Scientific and Production Center (Novosibirsk, Russia). This AgNP formulation has been previously characterized by transmission electron microscopy (TEM) and has been described to have a spheroidal shape with a diameter distribution between 1 and 90 nm, with an average size of 35 ± 12 nm. The hydrodynamic diameter is 70 nm, with a ζ potential of −15 mV, and a plasmon resonance found at 420 nm. This formulation is a stable aqueous suspension that contains 1.2% weight of metallic silver stabilized with 18.8% weight of PVP (12.6 ± 2.7 kDa, Boai NKY Pharmaceuticals, China). The final concentration of Argovit is 200 mg/mL (20%) AgNPs. 41 From here on, this formulation is identified as 35 nm PVP−AgNPs.
Besides, we used the commercially available AgNP formulation Eonix silver nanospheres dried powder (nano-Composix, San Diego, CA) under the same experimental conditions for comparative purposes. The size of this AgNP formulation reported by the provider for this batch is 51 ± 9 nm. The metallic silver content is 34% of the mass and 66% of PVP 40 kDa as the coating agent. Plasmon resonance was reported at 430 nm. The resuspension of AgNPs was carried out following the recommendations of the provider with distilled water to obtain a stock solution of 1.2 mg/mL. From here on, this formulation is identified as 50 nm PVP−AgNPs.
Cytokinesis-Block Micronucleus (CBMN) Assay on Human Lymphocyte Cultures. The cytokinesis-block micronucleus (CBMN) assay on human lymphocyte cultures was conducted as described by Fenech. 66 The first step consists of the addition of 0.5 mL of heparinized peripheral blood from a normocytic nonsmoking 38-year-old male donor to 6. The lymphocytes were incubated at 37°C and exposed to different concentrations of AgNPs: 0.012, 0.12, 1.2, and 12 μg/ mL. Dilutions were calculated according to the metallic silver content in the Argovit and nanoComposix formulations. Physiological solution and NaAsO 2 (0.12 μg/mL) (Sigma S7400, St. Louis, MO) were used as negative and positive controls of cytotoxic and genotoxic effects, respectively. In our experiment, NaAsO 2 was added 24 h later than the rest of the assayed compounds to avoid masking the genotoxic damage manifested as NBUDs and NPBs by its powerful cytotoxic effect. An additional experiment with PVP (0.0188 and 188 μg/mL) was included to differentiate the impact of the coating agent from that elicited by AgNPs. After 44 h of incubation, the addition of 21 μL of cytochalasin B (Sigma C6762, St. Louis, MO) at 2 mg/mL blocks cell cytokinesis (Figure 4). The cultures were incubated at 37°C for another 28 h until harvest. After 72 h of culture, the lymphocytes were harvested through centrifugation, fixed with a methanol ( where T is the test chemical treatment culture, C is the control culture, and MULTIN = trinucleated + tetranucleated cells.
The cytostasis percentage is defined as follows The same number of cells was used to quantify apoptosis and necrosis. 16 Frequencies of micronuclei (MNi), nuclear outbreaks (NBUDs), and chromatin bridges (NPBs) were quantified on 1000 binucleated (BN) cells with a wellpreserved cytoplasm to evaluate the genotoxic effect of each treatment. 17 The scheme in Figure 4 represents the procedure described above.
Statistics. To guarantee the homoskedasticity between the experimental groups, a square root transformation was carried out, 67 which is suggested for the variables with the goodness of fit to the Poisson model, such as micronuclei and other nuclear anomalies, 68 according to the following formulâ = + X X 1 2 i k j j j y { z z z (5) X̑is the estimator of the transformed variable and X is the original variable, number of counted anomalies. The transformed data were analyzed using the StatSoft and Graph-Pad-Prism v8. A Kruskal−Wallis test was performed with Tukey's post hoc multiple comparison test. * Indicates significant differences (p ≤ 0.05), ** indicates very significant differences (p ≤ 0.01), and *** indicates highly significant differences (p ≤ 0.001) compared with the negative control (lymphocytes without treatment), while § indicates significant differences (p ≤ 0.05) compared with the positive control (sodium arsenite). The p-value of each analysis is indicated on the top of the corresponding figure.