Temperature-dependent sex-reversal by a transformer-2 gene-edited mutation in the spotted wing drosophila, Drosophila suzukii

Female to male sex reversal was achieved in an emerging agricultural insect pest, Drosophila suzukii, by creating a temperature-sensitive point mutation in the sex-determination gene, transformer-2 (tra-2), using CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated) homology-directed repair gene-editing. Ds-tra-2 ts2 mutants developed as normal fertile XX and XY adults at permissive temperatures below 20 °C, but at higher restrictive temperatures (26 to 29 °C) chromosomal XX females developed as sterile intersexuals with a predominant male phenotype, while XY males developed with normal morphology, but were sterile. The temperature-dependent function of the Ds-TRA-2ts2 protein was also evident by the up- and down-regulation of female-specific Ds-Yolk protein 1 (Ds-Yp1) gene expression by temperature shifts during adulthood. This study confirmed the temperature-dependent function of a gene-edited mutation and provides a new method for the more general creation of conditional mutations for functional genomic analysis in insects, and other organisms. Furthermore, it provides a temperature-dependent system for creating sterile male populations useful for enhancing the efficacy of biologically-based programs, such as the sterile insect technique (SIT), to control D. suzukii and other insect pest species of agricultural and medical importance.

. Strategy for generating the Ds-tra2 ts2 mutation and IE1hr5-DsRed marker insertion via CRISPR/Cas9 homology-directed repair. (a) Schematic (not to scale) showing the wild type Ds-tra-2 gene and downstream HA2 (homologous arm 2) sequence with expected recombination sites (X) for the Cas9/sgRNA complexes (top). Dotted lines from these sites extend to the donor construct recombination sites that include the HA1 Ds-tra-2 sequence mutagenized in vitro to create a Pro191Ser substitution (vertical red line) resulting in the mutated Ds-TRA2 ts2 temperature sensitive protein, in addition to the IE1hr5-DsRed marker gene and HA2 sequence. The recombined donor construct resulting in the genomic insertion of the Ds-tra2 ts2 mutation and marker gene is shown below. (b) Ventral view of a homozygous Ds-tra2 ts2 adult XY male (left) and XX female (right) reared at 16 °C under brightfield, and (c) DsRed epifluorescence illumination. See Methods for further details.
be the biogenesis of lysosome-related organelles complex 1 subunit 1 gene (GenBank: XM_017072992). To bias editing towards HDR, the ligase 4 gene, a component of the non-homologous end joining (NHEJ) repair pathway, was suppressed using dsRNA-mediated RNA interference (RNAi). A mixture of sgRNAs, Cas9 protein, Ds-ligase-4 dsRNA and donor construct was injected into pre-blastoderm embryos of a D. suzukii wild strain, yielding 81 G0 adult flies (36 males and 45 females). Of these, 55 flies were fertile after individual backcrosses to wild type, with four G0 lines yielding G1 progeny expressing whole body red fluorescence, resulting in a germ-line transmission rate of 7.3% (4/55) based on marker expression. From each G0 line, two to five red fluorescent G1 flies (Fig. 1c) were selected to establish independent G1 lines. After being inbred to homozygosity, six lines were analyzed by PCR and sequencing showing that all lines had an insertion of the IE1hr5-DsRed marker gene, however, only three lines (F26m1, F26f4 and M36f1, from two G0 lines) carried the correct Ds-tra-2 ts2 (CCT > TCT) mutation while the other three lines could not be confirmed, resulting in a minimum mutation rate of 3.6% (2/55).

Temperature sensitivity of Ds-TRA-2 protein function based on adult morphology and fertility.
To determine the effect of temperature on the mutated Ds-TRA-2 protein in terms of morphological sexual differentiation in chromosomal females and males, the homozygous mutant line, M36f1, was first reared continuously at the expected permissive temperature of 16 °C, in addition to 20 °C. This resulted in normal development and fertility in all XX females and XY males at both temperatures ( Fig. 1b; Table 1). However, when rearing was performed at the expected restrictive temperature of 29 °C, all mutants died during late pupation, as did wild type control flies. Rearing at 27 °C and 28 °C also resulted in inviability for the mutant line and a low survival rate for wild type (1-2%), with low levels of survival (5-10%) for both wild type and mutant flies first observed at 26 °C. At this temperature 80 surviving chromosomal female XX; Ds-tra-2 ts2 homozygotes exhibited an intersexual, though predominantly male (or pseudo-male), phenotype ( Fig. 2c and f), but male-specific wing spots were not apparent (Fig. 2c). However, in a separate experiment where XX; Ds-tra-2 ts2 individuals (n = 21), were reared at 22 °C and shifted to 29 °C as late pupae, wing spot pigmentation was observed at 3 days after eclosion (Fig. 2c inset; Supplementary Table S1). Compared to mature ovaries from WT females (Fig. 2i), XX; Ds-tra-2 ts2 pseudo-males had a single poorly differentiated gonadal lobe (Fig. 2j) and no internal male reproductive organs, similar to XX; Dm-tra-2 ts2 flies reared at 29 °C 1 . At 26 °C pseudo-males were also sterile with fully differentiated male-specific foreleg sex combs (Fig. 3c) and male-specific pigmentation on tergite 6 ( Fig. 3f), while tergite 5 lacked male-specific pigmentation and female-specific tergite 7 was observed. The differing sexual identity of the abdominal tergites, as well as other sex-specific tissues and structures, can most simply be attributed to a variable sensitivity to Ds-TRA-2 function that was diminished in pseudo-males reared at a sub-optimal restrictive temperature.
A detailed examination of the external genitalia revealed that pseudo-males exhibited terminalia similar to WT males (Fig. 4), including the anterior paramere (clasper), posterior paramere, aedeagus and anal plates (Fig. 4c). However, the basal apodeme of the aedeagus was not apparent (Fig. 4a) and the female-specific sternite 8 was observed, while the female-specific spermatheca was not observed. At 26 °C, XY; Ds-tra-2 ts2 chromosomal males had normal external morphology, but were sterile consistent with poorly differentiated abnormal testes that had a small number of immotile sperm (n = 15, Fig. 2h, Supplementary Video S1), compared to the long spiral testes containing motile sperm found in WT males (Fig. 2g, Supplementary Video S2).
To more precisely determine the temperature sensitivity of Ds-TRA-2 function in the mutant line, adult morphology and fertility were assessed in XY and XX; Ds-tra-2 ts2 adults that developed at rearing temperatures ranging from 16 °C to 26 °C (Table 1). As noted, at the permissive temperatures of 16-20 °C both XX females and XY males were morphologically normal (Fig. 1b) and fertile, but at 22 °C XX females first exhibited male sex combs and were sterile based on a failure to oviposit (n = 61), while WT females oviposited ≥78 fertile eggs/ female (n ≥ 39) when reared at 20-24 °C ( some exhibiting dysmorphic testes and sterility at 24-25 °C, that included all males above 25 °C. These results are consistent with Ds-TRA-2 function, or the quantity of functional protein, diminishing just above permissive temperature, with an increasing loss of Ds-TRA-2 activity with rising temperature. The sex-specificity of somatic tissue and germ-line function appeared to be more highly sensitive to loss of Ds-TRA-2 activity in XX females (i.e., onset of sex comb differentiation and sterility) than the influence on testis morphology and germ-line function (i.e., fertility) in XY males where the onset of defects were first detected at a higher temperature. Complete sterility in XY; Ds-tra-2 ts2 males could be achieved by rearing at 25.5 °C or above, but viability at these temperatures was severely limited. Thus, XY; Ds-tra-2 ts2 males were reared at 22 °C until pharate adulthood or adult eclosion resulting in viability of 80 to 90%, and shifted to 26 °C and/or 29 °C to determine if these late shifts to restrictive temperature would result in sterility (Supplementary Table S1). Sterility was achieved in 90 to 100% of the males, with greater sterility in those that were shifted earlier and to the higher temperature, although this resulted in lower viability.

Mating behavior of mutant intersexes and pseudo-males.
In D. melanogaster, temperature sensitive male courtship behavior and mating in XX individuals required a nearly null Dm-tra-2 genotype that used the stronger Dm-tra-2 ts1 allele over a Dm-tra-2 deficiency 11 . For XX; Ds-tra-2 ts2 pseudo-males, flies reared at 26 °C (n = 20), and shifted to 29 °C for 3d at eclosion from 26 °C (n = 15) or as late pupae (n = 23) did not attempt courtship or mating with wild type virgin females, while XY; Ds-tra-2 ts2 males reared at 26 °C (n = 22) did mate, but were sterile. At 22 °C, XX; Ds-tra-2 ts2 females developed female genital terminalia and did not attempt courtship with WT virgin females (n = 29), but were courted by WT and XY; Ds-tra-2 ts2 males who were rejected (n = 20).
Temperature sensitivity of Ds-TRA-2 function based on terminal sex-specific Yp1 expression during adulthood. Similar to studies on XX; Dm-tra-2 ts2 in D. melanogaster 5 , the influence of Ds-TRA-2 activity on molecular and biochemical aspects of terminal sexual differentiation could be explored by applying temperature shifts to mutant adults. For D. suzukii in particular, the ability of adults to survive at 29 °C, after rearing at 26 °C or below, also allowed a more definitive test of the optimal restrictive temperature on Ds-TRA-2 ts2 protein function. This was achieved by assessing the effect of temperature and temperature shifts on the sex specific expression of the D. suzukii yolk protein 1 (Ds-Yp1) gene in Ds-tra-2 ts2 mutants that, in D. melanogaster, is normally expressed only in female adult fat body and the ovarian follicular epithelium beginning at adult eclosion 16,17 .  To measure Ds-Yp1 transcript levels by qPCR, Ds-Yp1 was first identified by its similarity to D. melanogaster Yp1, having 94.3% amino acid sequence identity ( Supplementary Fig. S1), providing primer sites for analysis. Initial qPCR tests for the primers showed that transcript levels were similar in XX WT and F23f1 control females, which were >20-fold higher than transcript in XX; Ds-tra-2 ts2 pseudo-male adults reared at 26 °C ( Supplementary  Fig. S2). For experimental tests, Ds-Yp1 transcripts were measured in XX; Ds-tra-2 ts2 (M36f1) phenotypic females and WT females reared and then maintained at 16 °C until 3 d, 6 d, and 9 d after eclosion ( Fig. 5a; Supplementary  Table S2). Transcript levels were not significantly different between the mutant and WT females at these time points, though transcript increased between days 3 and 6, and then decreased in both WT and XX; Ds-tra-2 ts2 females by day 9, though more so in M36f1 females (P > 0.05, two-tailed, no significant difference at all time points; Supplementary Table S2). In contrast, when mutant and WT females reared at 16 °C were shifted to 29 °C at eclosion, after 3 days WT females expressed >27-fold higher levels of Ds-Yp1 transcript compared to XX; Ds-tra-2 ts2 females (Fig. 5b). Decreasing levels of transcript occurred in both groups at 6 d and 9 d, with negligible transcript detected in mutant females after 9 d at 29 °C. To determine whether the basal transcript level observed in mutant females at 29 °C for 3 days could be reversed to a more normal female phenotype by turning on Ds-TRA-2 function, sibling flies were shifted to 16 °C at 3 d and assayed at days 6 and 9 after eclosion (Fig. 5c). At 6 d the Ds-Yp1 transcript level in WT females decreased ~40% (presumably due to a slowed metabolism), but increased ~9-fold in XX; Ds-tra-2 ts2 females, with similar levels maintained in both groups at 9 d (P > 0.05, two-tailed). However, if females at 6 d at 16 °C were shifted to 29 °C until 9 d, transcript levels decreased by half in WT females, but were barely detectable in the XX; Ds-tra-2 ts2 females, decreasing by >40-fold from day 6 (P < 0.05, two-tailed; Fig. 5d).
These results showed that adult XX; Ds-tra-2 ts2 phenotypic females reared at 16 °C exhibit normal female expression of Ds-Yp1, but when sibling females were shifted from 16 °C to 29 °C, low diminished transcript levels resulted reaching non-detectable levels, normally expected in XY males, by day 9. Furthermore, Ds-Yp1 transcript levels increased and decreased according to shifts in temperature to permissive and restrictive conditions, respectively, indicating that the dynamic, and reversible, expression of Ds-Yp1 during adulthood is continuously Figure 5. Response of Ds-Yp1 transcript expression to temperature. Ds-Yp1 transcript expression (relative to internal Ds-His3 controls) measured by qPCR from wild type females (WT-f, blue bars), XX; Ds-tra2 ts2 females (M36f1, red bars), and wild type males (WT-m, green bars) in response to indicated temperature and temperature shifts during adulthood. All flies were reared at 16 °C until adult eclosion, and time points indicate days from eclosion and temperatures maintained for the previous three days. Graphs show transcript levels from adults continuously reared at 16 °C (graph a) and 29 °C (graph b); adults reared from 0-3 days at 29 °C (3d-29 °C; from graph b) and then shifted to 16 °C for an additional 3 days (6d-16 °C) and 6 days (9d-16 °C) (graph c); or shifted to 29 °C at 6 days (9d-29 °C) (graph d; sibling samples from 3d and 6d in graph c shown for comparison). Each bar represents the mean fold change from three biological replicates ± SD relative to sample M36f1 at 3d-29 °C. Asterisks (*) above M36f1 samples indicate t-test significance (two-tailed) compared to WT-f at the same time point: ***P < 0.001; *0.01 < P < 0.05. Unmarked M36f1 data are not significantly different from WT-f. Transcript levels and P values are also presented in Supplementary Table S2. regulated by Ds-TRA-2 function. Presumably this function is mediated by the more direct effect of Ds-TRA/ Ds-TRA-2 interactions on the female-specific expression of Ds-dsx (GenBank: XM_017077018.1) 4 , as demonstrated in D. melanogaster 20 .

Discussion
Here we report the use of CRISPR/Cas9 gene editing in D. suzukii to create a conditional temperature sensitive allele of the tra-2 sex determination gene, Ds-tra-2 ts2 , analogous to the chemically induced Dm-tra-2 ts2 missense point mutation in D. melanogaster 1 . This was achieved by comparing amino acid sequence identity for TRA-2 in both species (Supplementary Fig. S3), and identifying the common peptides that are substituted in Dm-tra-2 ts1 and Dm-tra-2 ts2 1,18 . Since Dm-tra-2 ts2 was shown to exhibit better fertility than Dm-tra-2 ts1 at permissive temperatures 1,18 , its mutation site was the initial choice for editing in D. suzukii for this study, though the tra-2 ts1 allele might also present advantages for application, or additional insights into function.
Similar to Dm-tra-2 ts2 , XX chromosomal females homozygous for Ds-tra-2 ts2 reared at permissive temperatures of 16-20 °C resulted in development of normal fertile females, while rearing at a restrictive temperature of 26 °C resulted in a sterile, though incomplete pseudo-male phenotype. The lack of full male phenotypic differentiation, unlike XX; Dm-tra-2 ts2 pseudo-males reared at 29 °C, was most likely the result of rearing D. suzukii at a lower sub-optimal restrictive temperature, since this species fails to complete pupal development when reared above 26 °C. Thus, complete loss of Ds-TRA-2 function at 26 °C was unlikely, though distinct male morphological differentiation was apparent based on complete foreleg sex comb development, posterior dorsal tergite 6 pigmentation, and wing spot pigmentation when shifted to 29 °C in early adulthood. The genital apparatus structures were intersexual, but predominantly male-like, and similar to XX; Dm-tra-2 ts2 pseudo-males, the gonads of XX; Ds-tra-2 ts2 pseudo-males were dysmorphic, possibly intersexual, and did not produce motile sperm 1,19 . Conversely, XY; Ds-tra-2 ts2 males reared at 26 °C exhibited normal male external morphology and mated, but were also sterile with dysmorphic testes consistent with the tra-2 null phenotype in D. melanogaster that is known to interfere with male germ-line development 12,13 . A notable difference between the tra-2 ts2 lines in the two species is that Dm-tra-2 ts2 XX females and XY males were highly sensitive to loss of Dm-TRA-2 activity, becoming sterile at 18 °C 18 , while Ds-tra-2 ts2 XX and XY individuals exhibited less sensitivity, becoming sterile at 22 °C and 26 °C, respectively.
While D. suzukii could not develop past late pupation at 29 °C, surviving adults at 26 °C or below could be reared at 29 °C, allowing the sex-specificity of downstream transcriptional products in adults to be assessed in response to Ds-TRA-2 ts2 function at the optimal restrictive temperature. This allowed a more accurate comparison of TRA-2 function in D. suzukii and D. melanogaster and its downstream regulation of sexual differentiation. In D. melanogaster, functional TRA-2 acts in concert with the transformer gene product to facilitate the female-mode of doublesex alternative intron-splicing, resulting in DSX-Female function throughout adulthood 20 . The continuous need for control by the sex-determination gene pathway throughout female development was, indeed, first inferred by experiments with Dm-tra-2 ts2 that showed that female-specific Yp transcription and synthesis in adults could be up-and down-regulated by manipulating temperature-dependent Dm-TRA-2 function 5 . For Ds-TRA-2 ts2 , we showed that Yp gene expression could be similarly regulated by temperature such that XX; Ds-tra-2 ts2 pseudo-males reared at 16 °C and shifted to 29 °C at adult eclosion exhibited basal Yp1 transcription levels, that could be transiently up-regulated to levels comparable to WT females after a shift to 16 °C, and subsequently down-regulated to basal levels by a shift to 29 °C. Together, these studies confirmed the expressivity and temperature-dependent conditional function of the gene-edited Ds-tra-2 ts2 mutation that also provided support for the continuous, and reversible, requirement for Ds-TRA-2 in both females and males for adult sexual differentiation and fertility throughout development.
Beyond providing a conditional system to better understand how sex-determination genes control sexual differentiation and behavior, the Drosophila tra-2 ts studies also present a highly efficient strategy for generating a sterile male population for SIT 6 . For this, a tra-2 ts mutant strain reared at permissive temperature would result in a normal breeding population of fertile males and females, whose chromosomal male and female progeny all develop as sterile phenotypic males at the restrictive temperature. Thus, ideally, a tra-2 ts mutant strain could provide a complete sterile male population in response to a shift in temperature, and provide the significant efficiency of all zygotes being used for release, requiring a considerably smaller breeding population.
Current control of D. suzukii does not include SIT 21 , and the temperature-sensitive sexing system described is less than optimal for this species due to post-larval inviability at 29 °C required for complete XX female sex reversal. However, XY; Ds-tra-2 ts2 males are sterile but sexually active at ambient temperatures above 25 °C, and could be reared at lower temperatures for improved viability, yet sterilized when shifted to higher restrictive temperatures as pharate adults. These sterile males could directly compete with wild males in the field, though the ability of their sibling XX; Ds-tra-2 ts2 pseudo-males to provide mating competition remains uncertain. Regardless of their ability to mate with females in the field, chromosomal females that develop as phenotypic males or intersexuals would still preclude the need to eliminate or sterilize these individuals before release, unlike normal females, since they would not require sterilization, nor compete with females in the field for mating with released sterile males, or cause oviposition damage to host plants. Thus, the programmatic and cost advantages of Ds-tra-2 ts2 -based sexing and sterility might still provide a level of efficiency that would support its incorporation into prospective SIT programs for D. suzukii. Nevertheless, the fertility and mating behavior of both XX and XY mutant adults would require further testing to ensure the efficacy of this system in response to temperature fluctuations under actual field conditions. This demonstration of temperature-dependent sex reversal established by gene-editing in D. suzukii also supports the notion that this could be accomplished in other insect species having conserved tra-2 cognate function and sequence identity. This is especially so for several tephritid fruit fly species, such as Ceratitis capitata (medfly), Anastrepha ludens (mexfly), and A. suspensa (caribfly), which have conserved sites for the TRA-2 Alanine and Proline peptides substituted in the tra-2 ts1 and tra-2 ts2 mutations, respectively 3,22 . Importantly, sex reversal of chromosomal females by RNAi-based knock-down of the tra and tra-2 cognates, resulting in XX pseudo-males that copulate with wild females, has already been demonstrated for medfly and caribfly 22,23 . Given that these tropical insects thrive at ambient temperatures of 29 °C or above, it is likely that full male differentiation could be achieved in XX; tra-2 ts individuals with the potential for successful mating with females in the field. However, the RNAi studies also showed that while tra-2 knock-down in XX caribflies resulted in sterile pseudo-males, XY males remained fertile, and in medfly both XX pseudo-males and XY males remained fertile. Thus, for tephritid species tra-2 ts males reared at restrictive temperatures might remain fertile, requiring an independent conditional system for male sterility, such as the tetracycline-dependent embryonic lethality system described for medfly and caribfly 24,25 .
In summary, proof of principle has been demonstrated in D. suzukii for the creation of a conditional temperature-sensitive allele by gene-editing that most likely can be extended to other temperature-sensitive missense mutations, and in a wide range of species known to have an orthologous gene 26 . That a very broad range of genes can be edited in this way in insects is supported by D. melanogaster mutational studies that showed that greater than 10% of X-linked lethal and semi-lethal mutations are temperature-sensitive 27 . For the tra-2 gene in particular, that is conserved among several orders of insects, synthetic creation of temperature-sensitive mutations has significant potential for detailed comparative functional analyses and, for pest species, enhancing the efficacy of programs to control their population size. While this and other possible applications remain prospective, the ability to create conditional alleles in a large number of genes known to be important to many aspects of reproduction, development and behavior should have a highly significant impact on our understanding of genome function and evolution.

Methods
All data generated or analysed during this study are included in this published article (and its Supplementary Information files). All sequence files are available from the GenBank database (accession numbers MF142759, MF142760, MF142761 and MF142762) after publishing of this work.
Fly rearing, fertility and mating tests. A wild type strain of D. suzukii originally collected in Wimauma, Florida, was reared under standard laboratory conditions as described 28 . Gene-edited transgenic homozygous flies were reared at 16 °C ± 0.2 °C (EchoTherm ™ IN40 incubator, Torrey Pines Scientific, Inc.), and tested at indicated temperatures ( ± 0.2 °C) for temperature sensitivity after 24 h and 48 h of egg collection at 16 °C. Fertility tests were performed by mating each mutant male to five wild type virgin females (n ≥ 15) and each mutant female to three WT males (n ≥ 20) followed by egg collections for 12-15 days. Progeny were reared to adulthood and screened for red fluorescence. Mating tests were performed by combining previously unmated 3 d adult flies for observation of courtship behavior including body orientation, wing vibration, licking and copulation for 6 h at mid-day (1000-1600), and for 4 h beginning at dawn (approx. 0700) of the following day.

Mutation generation via CRISPR/Cas9. The Ds-tra-2 gene was identified by a Blast search using
Dm-tra-2 mRNA (GenBank: NM_057416.3) as a query, and was identified in contig1010 from the D. suzukii genome (GenBank: XM_017071951) 4 . The putative Ds-tra-2 ts2 mutation site was localized to nt 571 in the CDS (GenBank: MF142759) based on alignment with the Dm-tra-2 ts2 amino acid sequence 1,18 , and confirmed with primers AH1001/AH1002 by RT-PCR and sequencing. Oligonucleotide primers were designed with Geneious ® 8.0.5 (Biomatters Ltd.) software and listed in Supplementary Table S3 with annealing conditions. sgRNA targets were identified by a manual search for NGG proto-spacer adjacent motif (PAM) sequences. Two targets were identified having high GC content within the six PAM-proximal nucleotides (PAMPNs) 29 and less similarity to other genomic sequences to improve efficiency and avoid off-target effects. sgRNAs were synthesized as previously described 30 using primers AH992, AH1053 and AH1054 (Supplementary Table S3). Their specificities were confirmed by embryonic injections and Surveyor tests ( Supplementary Fig. S2).
To create the donor construct, a 3.6 kb fragment from D. suzukii genomic DNA was cloned using Q5 ® High-Fidelity DNA Polymerase (New England Biolabs) with primers AH1020/AH1021, which included two homologous arms of more than 1 kb upstream and downstream from the two sgRNA target sites. The Ds-tra-2 ts2 point mutation was introduced by primers AH1027/AH1028. To avoid cleavage of the donor construct and mutagenesis after integration by CRISPR/Cas9, two single-nucleotide synonymous substitutions (G > C for sgRNA1 site; G > A for sgRNA2 site) were introduced into the two sgRNA target site PAM sequences, respectively, since an intact PAM sequence is necessary for successful double strand breakage 31 . Homologous arm 1 (HA1) was assembled using the GeneArt ® Seamless Cloning and Assembly Kit (Thermo Fisher Scientific) from three fragments, which were amplified with primer pairs AH1026/AH1027, AH1028/AH1029, and AH1030/ AH1031. HA2 was assembled from two fragments amplified with primer pairs AH1032/AH1033 and AH1034/ AH1035 with the Ds-tra-2 ts2 mutation and PAM substitutions confirmed by sequencing. Next, a fragment containing the IE1hr5-DsRed/SV40 marker gene was amplified from the piggyBac transformation vector plasmid, pB{IE1hr5-DsRed/SV40} with primers AH1044/AH1045, and the complete HA1 and HA2 fragments were amplified using primer pairs AH1026/AH1043 and AH1035/AH1046. The DsRed marker, HA1 and HA2 were then assembled with the GeneArt ® Kit and confirmed by sequencing.
The D. suzukii Ds-ligase4 gene (GenBank: XM_017079597) 4 was identified by Blastn alignment similarity to Dm-ligase4 from D. melanogaster (GenBank: NM_132679.3; protein alignment of the conceptual translation of these sequences shown in Supplementary Fig. S4). The online SnapDragon tool (http://www.flyrnai.org/ snapdragon) was used to design a 421 bp dsRNA fragment whose sequence was confirmed after PCR amplification with primers AH1003/AH1004 (GenBank: MF142760). The dsRNA was then synthesized as described previously 22,32 .