Remodeling without Destruction: Nonproteolytic Ubiquitin Chains in Neural Function and Brain Disorders

Ubiquitination is a fundamental posttranslational protein modification that regulates diverse biological processes, including those in the CNS. Several topologically and functionally distinct polyubiquitin chains can be assembled on protein substrates, modifying their fates. The classical and most prevalent polyubiquitin chains are those that tag a substrate to the proteasome for degradation, which has been established as a major mechanism driving neural circuit deconstruction and remodeling. In contrast, proteasome-independent nonproteolytic polyubiquitin chains regulate protein scaffolding, signaling complex formation, and kinase activation, and play essential roles in an array of signal transduction processes. Despite being a cornerstone in immune signaling and abundant in the mammalian brain, these nonproteolytic chains are under-appreciated in neurons and synapses in the brain. Emerging studies have begun to generate exciting insights about some fundamental roles played by these nondegradative chains in neuronal function and plasticity. In addition, their roles in a number of brain diseases are being recognized. In this article, we discuss recent advances on these non-conventional ubiquitin chains in neural development, function, plasticity and related pathologies.


Introduction
Ubiquitination is an essential post-translational protein modification in eukaryotes through which the 76 amino acid protein, ubiquitin is conjugated to a protein substrate [1]. Ubiquitination is completed in sequential enzymatic events through E1 ubiquitin-activating, E2 ubiquitin-conjugating, E3 ubiquitin-ligating enzymes, and sometimes an E4 ligase, and is reversed by a family of deubiquitinases (DUBs) (Fig. 1a). A major form of ubiquitination is polyubiquitination where a chain of ubiquitin molecules linked through the C-terminal glycine of one ubiquitin molecule and an internal lysine of another ubiquitin molecule is conjugated on a lysine residue of a substrate. Given that seven internal lysine residues (K6, K11, K27, K29, K33, K48, and K63) exist in the ubiquitin molecule, seven distinct Diverse polyUb chains are "decoded" by a large number of UBD-containing proteins, known as ubiquitin receptors, which translate the ubiquitin code to specific biochemical and cellular outputs [2,45]. There are more than 20 structurally different UBDs in five subfamilies, α-Helix, Zinc Finger (ZnF), Ubc-like, pleckstrin homology (PH) fold, and other structures [2]. Individual UBDs typically bind ubiquitin with low affinities, and additional interactions between ubiquitinated targets and ubiquitin receptors in a multiprotein complex cooperate to provide sufficient specificity, avidity, and dynamic regulation. The formation of these "signalosomes" through recruitment of binding partners that harbor specific UBDs is a hallmark role of nondegradative polyUb chains.

Major Nonproteolytic Paradigms in Non-neurons
Signalosome assembly and kinase activation.
Roles of nonproteolytic polyUb in scaffolding signaling complexes and activating kinases are best illuminated in the classical NF-κB signaling pathways in innate and adaptive immunity (Fig. 2a) [18,[46][47][48]. Activation of Toll-like and cytokine receptors recruits, in a ligand-and receptor-specific manner, adaptor proteins, protein kinases, and a panel of K63-or M1-specific E3 ligases including TRAF6 and LUBAC, promoting their activation. Activated E3s coordinate with specific E2s to assemble K63 and M1 chains on specific substrates or free, unanchored chains. These polyUb chains then serve as scaffolds to simultaneously load the TAK1 (TGF-β activated kinase 1) kinase complex and IKK (Inhibitor-of-κB kinase) complex through their UBDs, leading to the activation of the IKK complex. Activated IKK complex then recruits a signaling complex consisting of IkBα (inhibitor of NF-κB) and NF-κB subunits p65/p50, leading to phosphorylation and proteasomal degradation of IkBα by the E3 complex SCF βTrCP [49]. Liberated NF-κB then enters the nucleus to regulate expression of diverse target genes important for inflammation and cell survival. The ubiquitin editing enzyme A20, an OTU DUB, and CYLD are potent inhibitors of NF-κB signaling by cleaving K63 and M1 chains [19,50].

Endocytosis:
Ubiquitination is a classical signal for surface receptor endocytosis, endosomal trafficking, and sorting [17,51,52]. Upon ligand binding, activated receptors are ubiquitinated at the plasma membrane by several K63 E3 ligases recruited to the activated receptors, such as CBL (Cas-Br-M ecotropic retroviral transforming sequence; a RING E3), TRAF6, and the Nedd4 family. AMSH (associated molecule with the SH3 domain of STAM; a JAMM DUB) and USP8/UBPY (ubiquitin-specific protease 8/ubiquitin-specific protease Y) negatively regulate endocytosis by cleaving K63 chains from internalized receptors [53,54]. Ubiquitinated receptors are internalized through either clathrin-dependent or -independent endocytosis, driven by a set of endocytic adaptors at the plasma membrane, such as EPS15 (epidermal growth factor receptor substrate 15) and epsins, which bind ubiquitin through their UBDs and couple receptors to endocytic vesicles. Once at early endosomes, ubiquitinated cargo is sorted through endosome-associated complexes ESCRT (endosomal sorting complex required for transport)-0, -I, -II, and -III into multivesicular bodies (MVBs) within late endosomes for degradation (Fig. 2b). Importantly, each ESCRT complex contains UBDs in their subunits that facilitate formation of multiprotein complexes necessary for efficient endosomal sorting. Ubiquitin is removed by ESCRT-III-associated DUBs prior to receptor entry into MVBs. Monoubiquitin and K63-polyUb chains often dominate in endocytosis, however, the latter is believed to be more effective [55].
activator/repressor status [74][75][76][77] . Recent work indicates that Shh/Gli signaling gradient is also fine-tuned by nonproteolytic ubiquitination during ventral neural tube patterning [78]. Specifically, RNF220, an E3 specifically expressed in the developing ventral neural tube, assembles K63 chains on all Glis, either in their activator or repressor forms, and the K63 chains promote Gli nuclear export and refine their gradients by limiting their nuclear levels. Loss of RNF220 leads to expansion of the GliA and GliR gradients and disruptions of progenitor patterning along the dorsal-ventral axis [78].

Axonal and Dendritic Outgrowth
In developing sensory neurons, neurotrophin signaling promotes axon outgrowth through clathrin-mediated endocytosis and signaling endosomes that are retrogradely transported back to cell bodies to support neuronal survival [79,80]. Zhou et al. (2007) showed that in addition to this "global" mechanism, neurotrophins also regulate sensory axon elongation and branching via a K63-mediated clathrin-independent TrkA endocytosis locally at the growth cone [81]. In cultured mouse embryonic dorsal root ganglion neurons, NGF stimulates K63 ubiquitination of Ulk1/2 (Unc-51-like kinase 1/2) by TRAF6 and recruitment of Ulk1/2 to the TrkA receptor complex via interaction with p62, routing NGF-bound TrkA to non-clathrin-coated vesicles that attenuates NGF signaling and allows filopodia withdrawal. This study highlights an important role for presynaptic endosomal trafficking in axonal development (Fig. 3). Knocking down Ulk1/2 leads to impaired NGF endocytosis, excessive axon arborization, and severely stunted axon elongation. Mice lacking Ulk1/2 in the CNS showed defects in axonal pathfinding and defasciculation affecting the corpus callosum, anterior commissure, and corticothalamic and thalamocortical axons [82]. However, it should be noted that these loss-of-function experiments did not directly address the role of K63 ubiquitination of Ulk1/2 in these deficits.
Another K63-dependent mechanism regulating neurite outgrowth is mediated by Muscleblind-Like Protein 1 (MBNL1). MBNL1 is a multifunctional protein regulating the transition between differentiation and pluripotency and the pathogenesis of myotonic dystrophy type 1 (DM1), which is associated with dendritic and synaptic transmission deficits at early stages [83]. MBNL1 undergoes K63 ubiquitination, which is required for its localization in the cytoplasm. Cytoplasmic, but not nuclear MBNL1 promotes axon outgrowth and neurite differentiation in hippocampal neurons [84].

Presynaptic Differentiation and Function
Endosomal trafficking at presynaptic terminals plays an important role in synaptic vesicle (SV) cycles during presynaptic development and functions [85][86][87]. The ubiquitin-binding protein Phospholipase A2 Activating Protein (PLAA) has been shown to act as a ubiquitin adaptor required for post-endocytic sorting of ubiquitinated presynaptic proteins from early endosomes to lysosomes for degradation during SV recycling [88]. Mammalian PLAA specifically recognizes K63-polyUb-modified SV components and targets them for ESCRT-dependent degradation via MVBs. Mice deficient in PLAA show disrupted Purkinje cell migration and dendritic arborization, accumulated K63-ubiquitinated proteins and presynaptic membrane proteins. This accumulation of K63-ubiquitinated proteins is thought to be a result of impaired trafficking of these proteins to ESCRT-dependent sorting and lysosomal degradation. Mutant NMJs show striking presynaptic swelling/sprouting, drastic loss of reserve pool SVs, and aberrant vesicle recycling during sustained activity. In both human and mouse, hypomorphic mutations in PLAA cause a lethal infantile neurodysfunction syndrome with epilepsy [88]. This study supports the importance of K63related UBD-containing ubiquitin adaptors and endosomal trafficking in SV homeostasis and presynaptic differentiation (Fig. 3).
In addition to its role in nuclear DNA repair in proliferating cells, RNF8 and associated K63-specific E2 Ubc13 regulate presynaptic differentiation in postmitotic neurons [89]. Knockdown or conditional knockout of RNF8 or Ubc13 in mouse cerebellum granule neurons robustly increases the number of presynaptic boutons and parallel fiber-Purkinje cell synapses as well as functional synaptic transmission. Knockdown of the K48-synthesizing E2 UbcH8, fails to increase the number of parallel fiber presynaptic boutons. Expression of an RNF8 mutant that interacts with Ubc13, but not UbcH8, rescues the number of presynaptic boutons. Cytoplasmic, but not nuclear RNF8 exerts this presynaptic differentiation role. RNF8 interacts with the HECT domain protein HERC2/scaffold protein Neuralized 4 (NEURL4) complex and knocking down either protein mimics the inhibition of RNF8 on synapse formation. These results, though short of directly showing involvement of K63 chains, strongly suggest that RNF8/Ubc13-dependent K63-ubiquitin signaling inhibits synapse formation in vivo (Fig. 3). Detailed molecular details of this K63-dependent presynaptic remodeling remain to be determined.
The metabotropic glutamate receptor 7 (mGluR7), a presynaptic G Protein-coupled receptor that inhibits glutamate release especially during sustained and heightened activity [90], is K63 ubiquitinated following agonist stimulation in heterologous cells and cultured neurons [91]. mGluR7 ubiquitination is mediated by Nedd4, which is recruited to activated mGluR7 by the adaptor protein β-arrestin, and leads to endosome-lysosome sorting and degradation of the receptor. This process may play a role in the regulation of presynaptic metaplastic long-term depression (LTD)/long-term potentiation (LTP) at certain synapses, e.g. mossy fiber-CA3 stratum lucidum interneuron (MF-SLIN) synapses [92] (Fig. 4; further discussed below).

Postsynaptic Function and Dendritic Spine Remodeling
Increasing evidence indicates that K63 ubiquitination occurs at mammalian synapses. A mass-spec analysis of rat brain ubiquitome shows that K63-polyUb species are the second most abundant ubiquitin linkage, behind K48 chains [16]. Mouse brain K63-polyUb levels increase during postnatal development and K63-polyUb clusters are abundant in dendritic spines in cultured rat hippocampal neurons [93]. K63-related ubiquitin enzymes, including TRAF3, TRAF6, Ubc13, Uev1A, CYLD, and A20 are present in the mouse brain postsynaptic densities (PSDs) [93][94][95]. Several signaling components of NF-κB pathway are also found at synapses [96][97][98]. These studies suggest that K63-polyUb may play synapseand neuron-specific roles in addition to their classical functions in host immune surveillance and defense.
Among K63-polyUb machineries localized in the PSD, CYLD emerges as a central player. CYLD transcript is high in fetal brain, and its mutations are associated with familial cylindromatosis, an autosomal dominant genetic predisposition to multiple tumors of the skin appendages [99]. CYLD protein is highly enriched in the rat brain PSD [93,100]. CYLD in the PSD is endogenously active with K63 deubiquitinase activity at basal conditions maintained by the PSD-associated IKK [100,101], and is recruited and further activated in a Ca2+-dependent manner by CaMKII to the PSD following high K+ or NMDA stimulation in cultured hippocampal neurons [102]. Thus, CYLD may participate in activitydependent regulation of K63 ubiquitination in synapses and synaptic plasticity ( Fig. 4; further discussed below). CYLD binds the scaffolding protein and autophagy receptor p62 [103], which is also abundant in the PSD. CYLD also interacts with Shank3, which regulates the abundance of CYLD and total K63-polyUb level in mouse striatal synaptosomes [104]. Proteomic analysis of CYLD interactome has identified more than 103 proteins, many of which are associated with ASD, schizophrenia, and depression. Despite its abundance and function in the PSD, it should be noted that a small amount of CYLD is present in the presynaptic fraction of the rodent brain [93], and a recent study reports that it regulates axonal length in cultured mouse hippocampal neurons [105].
In addition to its abundance and functions at excitatory synapses, CYLD also regulates inhibitory neurons and synapses [106]. In CYLD knockout mice, in vivo recording shows that the rhythmic activity in the striatum, where ~95% of neurons are GABAergic, is altered, characterized by shortened spontaneous up-states and increased membrane fluctuations preceding action potential firing. Levels of striatal GABA A and GABA B receptors are increased in mutant mice and pharmacological blockade of GABA receptors rescues the electrophysiological phenotypes. This study suggests that CYLD regulates synthesis, turnover, and/or trafficking of GABA receptors.
Recently, the ubiquitin-editing enzyme A20 has been shown to potently inhibit dendritic arborization, spine formation, and synaptic strength in cultured hippocampal neurons [95]. The A20 regulation of dendritic spine remodeling depends on the K63-specific DUB activity of A20 and is mediated by suppression of the (presumably nuclear) NF-κB signaling pathway. Thus, K63-linked ubiquitination can regulate postsynaptic remodeling via both local (synapse) and global (nuclear) mechanisms (Fig. 3).
Several ubiquitin enzymes have been identified to regulate AMPAR ubiquitination and trafficking. Nedd4 interacts with GluA1, promotes GluA1 ubiquitination, and is rapidly redistributed to spines in response to AMPAR activation in cultured neurons [115,126,127]. Overexpressing Nedd4 increases GluA1 ubiquitination, reduces its surface level, and enhances its internalization and accumulation at lysosomes, and Nedd4 mediates bicuculline-induced homeostatic synaptic downscaling [115,127,128]. Nedd4-2/Nedd4L, a closely related but functionally different E3, also ubiquitinates and downregulates GluA1 during synaptic downscaling [129]. RNF167, a plasma membrane-associated but predominately lysosomal RING E3 involved in endosomal trafficking, has been shown to ubiquitinate GluA2 and regulate synaptic AMPARs and activity-dependent AMPAR ubiquitination [117]. Two USP family DUBs can reverse GluA1 ubiquitination and may facilitate AMPAR recycling back to the plasma membrane. USP8/UBPY reduces basal and AMPA-induced AMPAR ubiquitination and positively regulates surface AMPAR levels, and is recruited to synapses following NMDAR activation in a Ca2+-dependent manner to regulate homeostatic downscaling [127]. USP46, a DUB that regulates GLR-1 trafficking and levels in C. elegans [130], has been reported to cleave K63-polyUb from AMPARs and regulate AMPAR internalization and synaptic strength in cultured rat cortical and hippocampal neurons [118].
Thus, surface AMPARs are ubiquitinated upon activation, internalized, and sorted to late endosomes and lysosomes for degradation or recycled back to the plasma membrane [112,113,131,132] (Fig. 5). However, it remains controversial whether AMPAR ubiquitination occurs before [115,127,128,133] or after [114,116] endocytosis. AMPARs can also be degraded by the proteasome system [128,134,135], especially in response to chronic elevation of synaptic activity [134], or by the autophagy-lysosome pathway during NMDAinduced chemical long-term depression (cLTD) [136]. Finally, although K63 chains are believed to be the primary polyUb chains conjugated to AMPARs [114,118] and guide AMPAR trafficking, further experiments are needed to elucidate the molecular details underlying each step of intracellular trafficking and sorting processes.

Synaptic Plasticity
In the mossy fiber-CA3 stratum lucidum interneuron synapses, sustained synaptic activity leads to activation of presynaptic mGluR7, which inhibits neurotransmitter release through PLC and PKC-dependent suppression of P/Q Ca2+ channels. mGluR7 activation unmasks the ability of these synapses to undergo LTP in response to the same high-frequency stimuli that induce LTD in naive slices [92]. K63 ubiquitination of mGluR7 routes it for lysosomal degradation [91]. These processes may regulate this metaplastic LTD/LTP switch (Fig. 4). Converging evidence also indicates that K63-polyUb mechanisms are regulated by neuronal activity and in turn regulate activity-dependent Hebbian and non-Hebbian synaptic plasticity. Brief exposure to NMDA, a cLTD-inducing stimulus, triggers a rapid and global disassembly of K63-polyUb chains in neuronal cultures and concomitant K63 deubiquitination of PSD-95, which is accompanied by dispersal of PSD-95 from synapses, internalization of AMPARs, and cLTD [93]. Deubiquitination of K63-ubiquitinated PSD-95 by CYLD is required for this cLTD. The activity-triggered, NMDAR-dependent CYLD synaptic translocation and activation [102] may serve as a potential mechanism for deubiquitination of synaptic substrates including PSD-95, and LTD (Fig. 4). Supporting the role of K63-polyUb in synaptic plasticity, Nedd4 heterozygous mice displayed a reduced hippocampal postsynaptic LTP [137], and theta burst stimulation (TBS)-induced LTP is absent in hippocampal CA1 neurons on slices from p62 knockout mice [138]. Finally, K63 ubiquitination, as discussed above, is an important signal for AMPAR trafficking that mediates bicuculline-induced synaptic downscaling, a non-Hebbian form of plasticity (Fig.  5) [115,[127][128][129].
The involvement of non-proteolytic ubiquitination in behavioral plasticity has also begun to emerge. Conditional knockout of RNF8 or Ubc13 in mouse granule neurons show cerebellar-dependent learning deficits [89]. Conditional knockout of cerebellar Ubc13 also produces disturbances in gait and spontaneous locomotion and exploration [139]. Nedd4 mutant mice show an impaired long-term spatial memory in the Morris water maze [137]. More directly, in the mouse amygdala, memory acquisition has been reported to be associated with a nuclear increase of K48, K63 and M1 chains, whereas memory retrieval induces an increase in these polyUb chains in the synaptic, but not nuclear or cytoplasmic regions [140]. As UPS and synaptic protein degradation underlies destabilization of retrieved fear memory [141], how proteasome-independent polyUb chains regulate memory formation, consolidation, and retrieval remain an important future question.
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Brain Diseases
In this section, we discuss emerging roles of nonproteolytic ubiquitination in several common neurodevelopmental, neuropsychiatric, and neurodegenerative diseases. Nonproteolytic ubiquitination related genes and their implications in each brain disorder are summarized in Table 2.

Autism Spectrum Disorders.
ASDs are neurodevelopmental disorders characterized by impaired social interaction and communications, as well as repetitive and stereotypic interests and behaviors [142]. Increased dendritic spine density is observed in frontal, temporal, and parietal lobes of some ASD patients [143,144], although decreased spine density has also been observed in ASD brains and animal models [145][146][147]. Genome-wide and homozygosity mappings in patients with autism have identified a deletion in the regulatory region of the RNF8 gene [148] and a homozygous missense mutation in the gene HERC2 [149]. RNF8 and HERC2, as discussed above, act to negatively regulate synapse density during development through a K63-dependent mechanism, and disrupting this mechanism results in learning deficits in mice [89].
degradation. Thus, USP7 appears to act as a "molecular rheostat" to regulate WASHdependent endosomal actin assembly and protein recycling via both K48 and K63 deubiquitination important in ASD pathogenesis [152].

Schizophrenia.
Schizophrenia is a severe mental illness that affects ~1% of the population worldwide, and is characterized by episodic positive symptoms (delusions, hallucinations, paranoia, and psychosis) and/or persistent negative symptoms (avolition, flat affect, social withdrawal) and cognitive impairments [157]. There is an overall reduction of protein ubiquitination, free ubiquitin, and K48-polyUb, but increased K63-polyUb in the superior temporal gyrus of schizophrenia subjects [158]. Ubiquitin E1 activating enzyme UBA6 and Nedd4 are also decreased, whereas the E2 ubiquitin conjugating enzyme UBE2K is unaltered. Given the important roles of Nedd4 in AMPAR ubiquitination and trafficking, a decreased Nedd4 level may contribute to glutamate hypofunction in schizophrenia. Single nucleotide polymorphisms in the Nedd4 gene have been associated with schizophrenia [159].

Alzheimer's disease.
AD is the most common neurodegenerative disease, characterized by progressive decline in memory, thinking ability, and cognitive functions. Neuropathological hallmarks of AD include intracellular, filamentous aggregates mainly consisting of hyperphosphorylated Tau (neurofibrillary tangles) and extracellular plaques enriched with Amyloid-beta (Aβ; amyloid plaques) [160][161][162]. Aβ, known to have numerous deleterious effects on neurons and synapses [163,164], is a peptide product generated from the cleavage of the Amyloid Precursor Protein (APP) by the β-(BACE) and γ-(presenilin) secretases after it exits the secretory pathway and reaches the cell surface [165][166][167]. APP is K63-ubiquitinated and sequestered early in the secretory pathway, primarily within the Golgi apparatus, delaying its maturation and subsequent proteolytic processing by secretases [168]. Ubiquilin-1, a ubiquitin-like molecular chaperone, stimulates this process [168]. Single nucleotide polymorphisms in the Ubiquilin-1 gene have been linked to late-onset AD [169,170] and Ubiquilin-1 levels are significantly decreased in late-onset AD patient brains [171]. Thus, although mutations in APP and the secretase enzymes often result in familial earlyonset AD [164,166], K63-polyUb may have a role in the pathogenesis of more common sporadic, genetically complex late-onset AD by regulating APP maturation, trafficking, and processing. Finally, presenilins can be K63-ubiquitinated by TRAF6, which does not appear to affect the γ-secretase enzyme activity but increases the levels and Ca2+ signaling properties of presenilins in heterologous cells [172]. Presenilin-1 also possesses a ubiquitin-binding CUE (coupling of ubiquitin to ER degradation) domain and can mediate a non-covalent binding to K63 chains [173]. The role of presenilin conjugation or binding by K63-polyUb in AD, if any, is currently unknown.

Frontotemporal Dementia (FTD).
FTD, the second most common cause of dementia after AD and the leading dementia before the age of 65, is caused by degeneration of prefrontal and/or anterior temporal cortex that leads to changes in personality, emotional blunting, disinhibition, and language disability [174][175][176]. FTD overlaps clinically, pathologically, and genetically with the motor Zajicek  neuron disease amyotrophic lateral sclerosis (ALS) [177]. TDP-43 (transactive response-DNA binding protein-43) is the major pathologic component of tau-negative and ubiquitinpositive inclusions in ~ 50% of FTD patients [175]. TDP-43 binds noncoding RNAs, introns, and mRNAs and regulates RNA splicing, trafficking, and expression of thousands of genes including Parkin [178,179]. TDP-43 is predominately nuclear, but in FTD/ALS, is translocated to the cytoplasm where hyperphosphorylated, ubiquitinated, and truncated TDP-43 is accumulated. It is currently unclear how cytosolic TDP-43 accumulation leads to pathogenesis, though it is hypothesized that it is mediated either by a toxic gain of function by aggregated TDP-43, or a loss of function by normal TDP-43 [180][181][182]. TDP-43 is K63ubiquitinated by Parkin, which does not seem to alter TDP-43 levels via autophagy process. Rather, Parkin forms a multiprotein complex with cytoplasmic HDAC6 (histone deacetylase 6) and TDP-43 that promotes TDP-43 translocation to and accumulation at the cytoplasm [183]. Furthermore, a mutation in the CYLD gene leading to increased K63-deubiquitinase activity of the protein is identified in FTD patients [105]. This mutant, when expressed in primary mouse neurons, results in increased cytoplasmic localization of TDP-43. This, in combination with CYLD's interaction with proteins encoded by several FTD-ALS linked genes including TBK1 [184,185], OPTN [186,187] and SQSTM1 [188,189], strongly suggest that CYLD has a causative role in the pathogenesis of FTD/ALS [105].
PD, the second most common neurodegenerative disease, is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of intracellular inclusions termed Lewy bodies (LBs). The major constituent of LBs is misfolded α-synuclein [190], an abundant lipid-binding presynaptic protein often conjugated with K63-polyUb [191]. Nedd4 has been shown to serve as an E3 for α-synuclein ubiquitination and facilitate its targeting to the endosomal-lysosomal pathway for degradation, promoting clearance of inclusions [192,193]. Independently, unbiased screens in yeast identified Nedd4 and related protein network as druggable targets that ameliorate α-synuclein toxicity in human neurons [194,195]. The deubiquitinase USP8, also present in LBs, removes K63-polyUb chains from α-synuclein and regulates its clearance and modifies its toxicity in PD [191]. These studies suggest that K63-linked ubiquitination of α-synuclein by Nedd4/USP8 represents an important mechanism in PD pathogenesis.

Concluding Remarks
The functional significance of nonproteolytic ubiquitination in the CNS is steadily emerging, and many fundamental questions need to be answered. Do other atypical polyUb chains have a role in neurons and synapses? How do different polyUb linkages coordinate to regulate activity-dependent assembly and disassembly of the PSD during synapse remodeling and plasticity underlying behavior? What are the precise molecular mechanisms by which K63-polyUb regulates each step of AMPAR trafficking and recycling? What are other synaptic substrates that undergo nonproteolytic ubiquitination and what are their roles? Answering these and other important questions will require the development of new tools for monitoring, manipulating, and characterizing different polyUb chains in the synapse and other specific neuronal compartments.
Zajicek and Yao Page 13 Mol Psychiatry. Author manuscript; available in PMC 2022 June 24. The brain was initially thought to be immunologically privileged, but it has become abundantly clear that many immune molecules are expressed in neurons and neuralimmune interactions profoundly impact synaptic development, function, and plasticity [196][197][198]. Importantly, abnormal immune activation in the brain, causing abnormal neural circuit rewiring and/or damage to neuronal integrity, is a common pathology across neurodevelopmental and neurodegenerative disorders [199,200]. Given the essential roles of nonproteolytic ubiquitination in innate and adaptive immune signaling, elucidating how these ubiquitin linkages regulate neural-immune interplays to mediate synapse remodeling in normal and disease states undoubtedly represents a fundamental endeavor.   Roles of nonproteolytic polyUb in signalosome formation and kinase activation. a, NF-κB signaling. Activation of TNF receptor (TNFR), interleukin 1 receptor (IL-1R), or Toll-like receptor (TLR) leads to, in a receptor-dependent manner, recruitment of adaptor proteins (TRADD, MyD88), protein kinases (RIP1, IRAKs), and E3 ubiquitin ligases (TRAF2/5/6, cIAP1/2, and LUBAC) which, together with specific E2s (not shown), catalyze the synthesis of anchored or unanchored polyUb chains of different linkages (K63, M1, and K11  Nonproteolytic ubiquitination in synapse development and remodeling. Presynaptically, the ubiquitin adaptor protein PLAA is required for sorting of ubiquitin-modified membrane proteins into the lumen of MVB/late endosomes. This process plays an important role in synaptic vesicle (SV) recycling, reserve pool size, synaptic transmission, and terminal differentiation. K63 ubiquitination also regulates filopodia extension and branching of sensory axons via an endocytic process. NGF induces K63-polyubiquitination of Ulk1/2 likely by TRAF6, allowing the binding of Ulk1/2 to p62 and the recruitment of Ulk1/2 to active TrkA complex via p62, leading to TrkA internalization, attenuation of NGF signaling, and filopodia withdrawal. RNF8/HERC2 may also regulate presynaptic differentiation in a K63-dependent manner through ubiquitination of unidentified substrates (S). Postsynaptically, A20 inhibits dendritic arborization and spine remodeling through suppression of NF-κB activity, likely via regulation of gene expression in the nucleus, causing postsynaptic remodeling. TRAF6 and CYLD control local postsynaptic remodeling through K63 polyUb chain conjugation and disassembly on PSD-95. PSD-95 is constitutively K63-ubiquitinated by TRAF6 in conjunction with Ubc13/Uev1A, and deubiquitinated by CYLD. The K63-polyUb conjugation sites are localized primarily in the GK domain, where the assembled K63 chains are essential for PSD-95 interactions with its GK binding partners including SPAR, GKAP/SAPAP, and AKAP79/150. K63-ubiquitinated Zajicek and Yao Page 28 Mol Psychiatry. Author manuscript; available in PMC 2022 June 24.
PSD-95, but not its un-ubiquitinated form, is targeted to the PSD to promote synapse efficacy (AMPAR numbers) and maturation. CYLD removes K63-polyUb from PSD-95, leading to translocation of deubiquitinated PSD-95 away from the PSD, destabilizing the PSD, weakening the synapse, and inhibiting synapse formation and maturation. PDZ, SH3, and GK domains of PSD-95 are indicated. PSD-95 binds NMDARs directly and AMPARs through Stargazin and localizes these receptors at the synapse.
Zajicek and Yao Page 29 Mol Psychiatry. Author manuscript; available in PMC 2022 June 24. Nonproteolytic ubiquitination regulates Hebbian synaptic plasticity. Presynaptically, sustained synaptic activity leads to activation of presynaptic mGluR7, which inhibits neurotransmitter release through PLC and PKC-dependent suppression of P/Q Ca2+ channels. K63 ubiquitination of mGluR7 routes it for lysosomal degradation, whereas K48 ubiquitination targets it for proteasomal degradation. These processes may regulate a metaplastic LTD/LTP switch at MF-SLIN synapses. Postsynaptically, IKK regulates constitutive CYLD activity through phosphorylation under basal conditions. NMDARmediated Ca2+ influx activates CaMKII, which further activates CYLD through phosphorylation, and/or recruits CYLD to the PSD. CYLD deubiquitinates PSD-95 and causes dispersal of PSD-95, leading to loss of synaptic AMPARs and weakening of synapses.
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Zajicek and Yao Page 31 Mol Psychiatry. Author manuscript; available in PMC 2022 June 24.