Study on tumor cell RNA cross-anticancer mechanism in non-tumor mice experimental model

To report the experiments of fractal structure and self-similar function in biological coupled oscillation brings life system waving growth in life organism. At the same time by self-organization the system openness come into being to maintain stability features of growth survival. From this, the orderly oscillation of tumor cell RNA was recognized that this process could lead to system sequentiality openness. According to waving growth of basic research the procedure could be sure to turn into a model as out of control of growing in tumor. Then in complete contrast that derived from the same component which have cross resistance effect. It shows that its biological behavior is converted as only a single physical power to play action by inactivated processing. Subsequent further confirms the essential roles whether essential roles link up with recovery of physiological function. The result in that it could exhibit feature of anticancer owing to be endowed tissue embryonization response. So that it also exerts preventing on senile chronic diseases. In theory, the concept of this tissue response in life organism closely link up with the single physical power of RNA component so as to bring up specific procedure of growing and subsisting that deal with keeping life vigor generalized explanatory.


INTRODUCTION
The reason why cancer is called an "incurable disease" is due to lack of fundamental understanding of oncogenesis [1][2]. Over the past forty years on both the scale and the scope of scientific research on cancer had reached an unprecedented level, with the emphasis on cancer vaccine [3], new drug and research on new medical technology. It has constructed a new science in contemporary medicine. The studies on biological wave started since the second half of 20th century [4]. The experiments at earlier stage were based on random operations, by using lysogeny broth (LB) medium and adding 2, 3, 5-triphenyl tetrazolium chloride (TTC) as a growing reaction reagent, to observe swarming growth of proteus 3 mirabilis via biological coloration as shown in Fig. 1. The same kind of bacteria appearance as two different colonies was regarded as phase variation phenomenon in the process of bacterial growing. The result caused by adopting connecting culture technique has shown that is a process of limitless growing. Till the basis of single bacillus population biological wave mathematical model established by Jiuping Xu et al [5][6], the compound noun "biowave" of biological wave started to be used.
From then on, the research on human biowave testing technology [7] has been set up. The neutrophilic granulocyte in blood was selected to do the test targets, using its characteristic of being sensitive to represent its integral change in real time. This sensitivity can also be shown as an enzymatic reaction to the redox of individual cell. The test used (3-(4,5-dimethy thiazolyl-2)-2,4-diphenyl tetrazolium bromide)(MTT) as hydrogen acceptor and formed colored formazan in the cell, so as to count the cellular rate in the positive or negative reaction. It represents the strength of integral cell population redox reaction. The study of biowave emphasizes the role of cell membrane permeability to the biochemical reaction of oxygen intake. In living organism the cell population had been measured to determining the base proportion changes at different times. The quantitatively elucidated ratio of redox changing dynamic process is termed "human biowave" dynamics [8]. The random change of biowave dynamic state could reflect the uncertainty 4 characteristics in biology. Thus the chaotic concept of life organism was introduced into the research field at that time. This kind of uncertainty of life organism resulted in biowave effect time uncompressing technique arose. By the first time, it made human experimental forecast come true.
Human biowave experimental forecast research results [9] showed that the occurrence or change of tumor is accompanied by biowave dynamic corresponding change. Thus it would establish technical conditions for studying the link between cancer and life organisms. For this reason, the research was converted from biowave basic theory to build the "non-tumor mouse" experimental model. To further clarify the stability change of biowave in inoculated tumor cells before and after. This will create conditions for further understanding of the life organism's response to different tumor cells and tumor cell components of experimental operation processing. This is good for clinical exploration of cancer. In systematic study, the result astonish that there is not only the growth of the tumor between the life organism and the cancer cells correlation, but will also the life organism can produce the anti-cancer ability. The research on the combination of biowave basic experiments and non-tumor mouse model shows that certain ingredients of tumor cells can also emerge anti-cancer promotion ability. And even more amazing is cross-resistance can occur among different types of tumor cell lines. As the research further develops this nonspecific effect property that directly 5 point to a new field arises. Basic experiment makes clear that the efficacy of anticancer mainly rests with the potential effect of tumor cell RNA constituent. In view of the RNA constituent special preparation as an inactivated RNA constituent result in pathogenic ability of malignant proliferation is lost. So it tentatively is called as "inactivated RNA vaccine" or the RNA for short. According to the basic experimental analysis, the basic mechanism be identified that the key factor is from the aid of life organism vitality. Thus，the RNA could keep happening coupled oscillation sequence characteristic. Subsequently the system is in the negative entropy flow dynamic process. As thus it can quantitatively express the effect of the RNA in the same way. So it can have been verified by life organism biowave effect time uncompressing assay in cancer incidence investigation. It has been revealed the RNA possesses the forcefully the organism biowave regulating effect that maintains the biowave stable and strong dynamic tendency. According to the basic experiment analysis suggests that this effect rests with the coupled oscillation dynamic process of molecule structure. The importance of this result is to promote system openness in life organism. Analysis identification that the condition of openness system can not only eliminate the carcinogenesis ecological niche, but also prevent tumor recurrence in situ as well as distant metastasis after surgical removal. And even the more important is the RNA could strengthen physical vitality for 6 the advanced cancer patient. So much that could improve the endurance in the process of surgery. In further explored the action mechanism of the RNA, the result had involved the RNA efficacy originated from the biological behavior of the inactivated RNA structure as single physical properties. It is this has been converted characteristic that could be possible to have such a special biological nature. Compared to the traditional basic medicaments the tumor cell RNA gives evidence the omnibearing efficacy in whole body. The effective fast and persistent as well as more targeted to prevent and control cancer.

Waving Growth Culture
Adopt LB solid medium add 0.05% TTC. Point-inoculate the strain onto a center of the plates. The incubator conditions have been regulated as temperature of 32 -39 ℃ , relative humidity 40%, and keeping ventilation.

Waving growth
The red bacterial colony is called central colony, came into being in 8 hours. Later on, yellow concentric ring bacterial colony encircles the red central colony formed. Thereby, it began to start the alternative waving growth process of yellow and red concentric ring bacterial colony every 7 three hours, as shown in Fig. 1.

Fig. 1 the dynamic biowave growth
As picture above showed that in the bacterial phase variation， the red colony is the vegetative cell and the yellow is the cryptic growth cell. The red central colony is the basis of waving growth. The size and shape of its colonies decide the subsequent dynamic condition of waving growth ring.
It reflects the "starting control effect" of waving growth and shows the self-similarity characteristic in the growth system. In the process it Combining with the distinct appearances of the micro-environments of colony related medium, it has further defined the biological phase variation process from red ring to yellow ring. So it is accompanying with the change of biological basic feature called as the phase variation.
Before it the bacterium is in vegetative phase as basic form, while after phase variation the bacteria appearing slender shape also known as filaments peritrichous flagellum called as bacterial "cryptic growth cell".
It has active movement and the biochemical metabolism had changed accordingly at the same time, as shown in Fig. 2  One is the quick waving growing concentric ring colonies which are pointed in the center; the other is the fan-like colonies which are located on the fifth position. The interval between both of the waving rings has obvious differences but meanwhile remains unchanged.
In the subsequent experiment [10], quick and slow waving growing strains were selected and the two kinds of bacteria were mixed with the proportion of 73:100, followed by making the mix strains point inoculation and cultivating their waving growths. The specimen from first to sixth concentric ring colony was collected from each of them, and the dynamic change of the two bacterial quantity ratios in the process of

Establishment of non-tumor mouse experimental model
In biowave basic experiment shows that both biotic communities from reproduce of the same species were continuously alternating. These

Preclinical trial indicators
To study the RNA vaccine for preclinical trial the experiment shifted to the indicators of preparation. The preclinical trial research should be direct beginning from the basic standards of the RNA. Especially the experimental models of non-tumor mouse must be as clinical set of rules to follow.

Evaluation of preliminary effect
It includes the pharmaceutical indicators that to determine water evaporation index. It is as to adjudge the micro-turbulence index of the RNA preparation.