Ochratoxin A induces ER stress and apoptosis in mesangial cells via a NADPH oxidase-derived reactive oxygen species-mediated calpain activation pathway.

Ochratoxin A (OTA) contaminated food increases reactive oxygen species (ROS) production in glomerulus and causes glomerulopathy. The molecular mechanisms still remain uncertain. In this study, we used mouse and rat glomerular mesangial cells and delineate the signaling pathway behind the OTA-triggered cell apoptosis. OTA dose-dependently induced expression of ER stress markers including phospho-PERK, phospho-eIF2α, GRP78, GRP94, and CHOP. Apoptosis events including cleavage of caspase-12, caspase-7, and PARP are also observed. OTA activated oxidative stress and increased NADPH oxidase activity. NADPH oxidase inhibitor, apocynin, significantly attenuated OTA-induced cell apoptosis. Moreover, OTA markedly increased the calpain activity which significantly inhibited by apocynin. Transfection of calpain-siRNA effectively inhibited the OTA-increased ER stress-related protein expression. These findings suggest that OTA activated NADPH oxidase and calpain, induced ER stress and ROS production, and caused glomerular mesangial cells apoptosis which leads to glomerulopathy.


INTRODUCTION
Ochratoxin A (OTA), a widely-spread mycotoxin produced by fungi, is a contaminant in the food chain worldwide. OTA is a secondary fungal metabolite and is known to be nephrotoxic. It has been suspected to be a major etiological substance for Balkan endemic nephropathy (BEN) and urinary tract cancer [1]. The pathological characterizations of BEN include a progressive atrophy and sclerosis in the kidney [2]. The glomerular and vascular lesions can also be observed in BEN, which include ischemic, microcystic, and obsolescent glomeruli, occasional thrombotic microangiopathy-like lesions, and focal segmental sclerosis-like lesions [2]. The renal lesions in porcine nephropathy, which OTA is a major causal determinant, are also characterized by the degeneration of the proximal tubules, interstitial fibrosis and hyalinization of the glomeruli [3]. The regional thickening and degeneration of the glomerular basement membrane has also been found in broiler chicks fed OTA [4]. Recently, Ciarcia et al. has shown that OTA treatment presented hypertension and reduction of glomerular filtration rate in rats [5]. However, the molecular mechanisms involved in the OTA-induced glomerulopathy still remain uncertain.
OTA induced oxidative stress and apoptosis in renal proximal tubular cells [6], monkey and human kidney epithelial cells [7], and kidney in vivo [8]. Endoplasmic reticulum (ER) stress-induced renal cell apoptosis caused some kidney diseases [9]. Mycotoxin patulin induced human intestinal and kidney cell cytotoxicity through

Research Paper
an oxidative stress-related induction of ER stress and mitochondrial apoptosis pathway [10]. The roles of oxidative stress and ER stress in OTA-induced glomerular mesangial cell cytotoxicity still remain unclear. In this study, we investigated the cytotoxic effect and molecular mechanism of OTA on glomerular mesangial cells. The involvements of oxidative stress, ER stress, and apoptosis in OTA-triggered mouse and rat mesangial cell cytotoxicity were tested.

OTA decreased cell viability and induced apoptosis and markers for ER stress and apoptosis in mesangial cells
We first tested the effects of OTA on cell viability and apoptosis in mesangial cells (MMCs and RMCs). OTA (10-50 μM) dose-dependently decreased MMCs and RMCs cell viability after 24 h exposure ( Figure 1A). Annexin-V/PI staining also showed that OTA effectively induced apoptosis in MMCs and RMCs ( Figure 1B).
Excessive ER stress triggers cellular apoptosis. We further to examine whether induced ER stress was essential for OTA-induced apoptosis. The markers for ER stress and apoptosis, such as phospho-PKR-like ER kinase (p-PERK), phospho-eukaryotic initiation factor-2α (p-eIF2α), GRP78, GRP94, CHOP, and cleavages of caspase-12, caspase-7, and PARP (poly-ADP-ribose polymerase) were investigated. As shown in Figure 2A and 2B, MMCs were treated with OTA (10-40 μM) for 4-12 h. OTA markedly induced the expressions of ER stress and apoptosis makers in MMCs in a dose-and time dependent manner.

OTA stimulated ROS production and NADPH oxidase activity in mesangial cells
Next, we determined whether OTA stimulates ROS production in mesangial cells. As shown in Figure 3A, OTA (20 and 40 μM) induced ROS generation in MMCs as early as 15 min and gradually increased up to 6 h. Similarly, treatment of OTA (20 μM) in RMCs for 1 h also increased the ROS production ( Figure 3B). NADPH oxidase is the major sources of ROS in variety of cells. We further determined whether OTA stimulated ROS generation via the NADPH oxidasedependent pathway. As shown in Figure 4A, OTA (20 μM) significantly increased the NADPH oxidase activity in MMCs and RMCs in a time-dependent manner. NADPH oxidase inhibitor apocynin significantly and dose-dependently inhibited the increased NADPH oxidase activity in MMCs and RMCs treated with OTA ( Figure 4B).

Apocynin attenuated OTA-induced cell death and apoptosis
We next investigated whether NADPH oxidasedependent ROS production was involved in the OTAinduced mesangial cell cytotoxicity. To address this issue, we determined the effects of apocynin on cell growth and apoptosis in OTA-treated mesangial cells. Bright field image observation revealed that apocynin (5 and 10 mM) protected cell growth from OTA (20 and 40 μM)induced cytotoxicity in MMCs ( Figure 5A). Treatment of OTA (20 and 40 μM) resulted in a suppression of cell proliferation determined by [ 3 H]thymidine incorporation in MMCs ( Figure 5B). Pretreatment ofapocynin (5 and 10 mM) could significantly inhibit the OTA-decreased cell proliferation in MMCs ( Figure 5B). Apocynin (5 and 10 mM) had no effect on the basal proliferation of MMCs. Moreover, apocynin (5 mM) could also inhibit the OTA (40 μM)-induced cell apoptosis in MMCs ( Figure 5C).

OTA induced calpain activity in mesangial cells, which could be inhibited by apocynin
We next investigated the effect of OTA on calpain activity in mesangial cells. As shown in Figure 6, OTA (20 and 40 μM) significantly increased the calpain activity in MMCs ( Figure 6A and 6B) and RMCs ( Figure 6C). Apocynin (2.5-10 mM) significantly reduced the OTAincreased calpain activity in mesangial cells ( Figure 6B and 6C).
The activity of calpain is as a key event in a variety of disorders, which connect to ER stress. Therefore, we next examined the role of calpain in OTA-induced markers for ER stress and apoptosis. The siRNA-mediated gene-silencing to knockdown calpain in mesangial cells was used. As shown in Figure 7, transfection of calpain siRNA led to a significant abatement in OTA-activated ER stress markers (p-PERK, p-eIF2α and GRP78) ( Figure 7A) and proapoptotic molecules (cleavages of caspase-12, caspase-7, and PARP) ( Figure 7B). These results demonstrated that NADPH oxidase was involved in the OTA-activated calpain activity-induced ER stress and apoptosis in mesangial cells.

DISCUSSION
OTA is produced by fungi from improperly stored foods. It has been analyzed that the average weekly intake of OTA varies from 130 to 6489 ng in inhabitants from an area with high BEN incidence in Bulgaria [11][12]. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) has established a provisional tolerable weekly intake (PTWI) for OTA of 100 ng/kg bw/week [13]. A prevalence of blood OTA levels exceeding 2 ng/ml  was found in BEN-affected families in Bulgaria [14]. A high level of serum OTA (1.8 μg/ml) has been found in one of the Croatian samples [15]. The concentrations of OTA in serum and kidney tissue of pigs from endemic areas of Bulgaria were 27-249 ng/ml (66.8-616 nM) and 1.32±1.25 μg/kg, respectively [16][17]. It has been reported that blood levels of OTA are 0.7-7.8 ng/ml in the general population and 12-55 ng/ml in the patients with chronic renal failure in Tunisia [18]. Experimental feeding of OTA (0.5 mg/kg), which total plasma concentration of OTA was 12.2±0.44 μM, has been shown to cause a reduction of glomerular filtration rate (GFR) and of paraaminohippuric acid clearance in pigs [19]. Treatment of OTA (0.5 mg/kg, i.p.) in rats could also lead to a reduction of GFR [20]. The cellular and molecular mechanism of OTA-induced glomerulopathy needs to be clarified. In the   5 mM). B. NADPH oxidase activity was determined by chemiluminescence assay as described under Material and Methods. All data are presented as means ± SEM from three independent experiments performed in duplicate. * p < 0.05 as compared with control. # p < 0.05 as compared with OTA alone. www.impactjournals.com/oncotarget present study, we found that OTA was capable of inducing cytotoxicity in glomerular mesangial cells. An oxidative stress-stimulated ER stress-related cell apoptosis pathway was involved in the OTA-triggered mesangial cytotoxicity.
OTA has been suggested to act on different sites along the nephron according to the evidence of pathophysiological investigations [21]. In the kidney, ROS can be generated in various cells [22]. The IC50 for cell viability of OTA in the proximal tubule cells and LLC-PK1 cells under normal medium was approximately 50 μM; the increased ROS contributed to the OTA-induced proximal tubular cytotoxicity [6]. OTA (5-40 μM) treatment has been found to activate signal-regulating kinase 1 (ASK1), increase ROS generation, and suppress cell viability in  human embryonic kidney cells HEK293 [23]. It has been shown that OTA at a concentration of 1 μM significantly decreases the protein content in human mesangial cells; the cellular mechanism remains unknown [24]. OTA metabolism through cytochrome P450 requires high level of nicotinamide adenine dinucleotide phosphate (NADPH) which leads to activated pentose phosphate pathway (PPP) [25]. Excess of glucose entry is diverted through the PPP which possibly provided additional substrate for the enzyme NADPH oxidase [26]. Moreover, in the kidney, NADPH oxidases are known to be a distinct cellular localization [22]. It has been shown that the predominant source of superoxide production in the rabbit renal cortex is NADPH oxidase [27].
In the present study, we found that OTA at the concentrations of 10-50 μM was capable of inducing cytotoxicity and cell apoptosis in mouse and rat mesangial cells. OTA could also induce ROS generation and NADPH oxidase activity. NADPH oxidase inhibitor apocynin significantly inhibited the OTA-induced mesangial cytotoxicity and apoptosis. Several studies have demonstrated that apocynin has therapeutic effect on multiple animal disease model including chemicallyinduced colitis in mice [28], testicular ischemiareperfusion injury in rats [29], bleomycin-induced lung fibrosis in rats [30], Cyclosporine A-Induced hypertension and nephrotoxicity in rats [31], established alcoholic steatohepatitis rat model [32], and contrastinduced nephropathy in the diabetic rats [33]. Therefore, these findings indicate that the concentrations of OTA used in this cellular toxicological study are reasonable and effectively induce mesangial cell cytotoxicity via a NADPH oxidase-derived ROS-mediated pathway.
ER stress can be induced in various renal diseases. ER stress-induced renal cell apoptosis is known to be one of the major causes of certain renal diseases [9]. Mycotoxin patulin (5 to 25 μM) has been shown to induce cytotoxicity in human colon carcinoma HCT116 cells and embryonic kidney HEK293 cells through a ROS-induced ER stress and induction of mitochondrial apoptotic signaling pathway [10]. Mycotoxin zearalenone (10-100 μM) could also induce cytotoxicity in RAW 264.7 macrophages through an ER stress pathway [34]. Moreover, calpain, an intracellular Ca 2+ -dependent cysteine protease, has been indicated to be associated with renal cell death induced by renal toxins [35][36]. Calpain activation has also been suggested to contribute to the ER stress-associated renal cell death following renal toxicants exposure [37]. Previous studies showed that increased ROS induces calpain activation in retinal photoreceptor cells which trigger the cell apoptosis [38]. ROS can target ER-based calcium channels leading to increased release of calcium into cytosol [39]. Since NADPH oxidase is the major ROS production enzyme inside cells therefore we hypothesized that NADPH oxidase activates calpain through ROS-induced calcium accumulation in cell cytosol. In the present study, we found that OTA (20-40 μM) effectively activated calpain activity in mesangial cells, which could be inhibited by apocynin. Transfection of calpain siRNA significantly inhibited the OTA-induced ER stress and apoptosis-related signaling molecules. These results suggest that calpain activation is involved in the induction of ER stress and apoptosis in OTA-treated mesangial cells.
In conclusion, this study demonstrated for the first time that OTA is capable of inducing oxidative stress, ER stress, apoptosis, and cell death in glomerular mesangial cells. NADPH oxidase and calpain activation are elevated in OTA-induced ER stress and apoptosis/cell death in mesangial cells, which may be involved in the pathogenesis of OTA-induced glomerulopathy.

Cell culture
Cell line murine glomerular mesangial MES-13 cells (MMCs) and primary rat mesangial cells (RMCs) were used in this study. Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, and 14 mM HEPES was used to culture cells unless otherwise stated. Cells were routinely passaged by trypsinization after they reached 80% confluence using 10-cm culture dishes and incubating them at 37°C in a humidified chamber with a 5% CO 2 /95% air mixture. RMCs were obtained by culturing glomeruli isolated from kidneys of 100-to 150-g male Sprague-Dawley rats by conventional sieving methods as described previously [30]. Cells were cultured in DMEM containing 20% fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, 44 mM NaHCO 3 , and 14 mM HEPES.

[ 3 H]Thymidine incorporation
DNA synthesis was measured by incorporation of [ 3 H]thymidine into cellular DNA. Cells were seeded in 96-well microtiterplates in a density of 5 × 10 4 cells/ ml in medium containing 5% FBS. After attachment of the cells overnight, [ 3 H]thymidine was added to the medium (1 μCi/ml), and then cells were incubated for an additional 18 h. At the end of the labeling period, the cells were washed twice with PBS, and harvested onto glass filters with an automated 96-well glass fiber harvester (PerkinElmer, Waltham, MA, USA). The radioactivity retained on the filter was measured by a scintillation β-counter (PerkinElmer).

Annexin-V FITC and PI double staining
The annexin V/propidium iodide (PI) (Takara Bio, Mountain View, CA, USA) was used to quantify numbers of apoptotic cells. Cells were washed twice with PBS and stained with annexin V and PI for 20 min at room temperature. The level of apoptosis was determined by measuring the fluorescence of the cells by flow cytometry analysis. (Becton Dickinson, San Jose, CA). Data acquisition and analysis were performed by the CellQuest program (Becton Dickinson).

Measurement of NADPH oxidase activity
Superoxide production was measured in total cell homogenates by using lucigenin-derived chemiluminescence as described previously [31]. Briefly, 50 μg of protein was diluted in 500 μl of 50 mM phosphate buffer containing 1 mM EGTA and 150 mM sucrose. Dark-adapted lucigenin was added to the sample, and chemiluminescence measurement was immediately started. Chemiluminescence (in arbitrary units) was measured at 15 s intervals for 1 min in a Tropix TR717 luminometer. NADPH (100 μM), a necessary substrate for NADPH oxidase, was added to optimize the detection NADPH oxidase-related superoxide anion. The protein levels were measured by the BCA Kit.

Calpain activity assay
Cells were cultured in 24-well plates. Cells were loaded with 40 μM Suc-Leu-Leu-Val-Tyr-AMC (a calpain protease substrate) and treated with OTA for indicated timing at 37°C in a humidified 5% CO 2 incubator. Quantitation of 7-amino-4-methylcoumarin (AMC) fluorescence can be used to measure enzyme activity. Proteolysis of the fluorescent probe was monitored using a fluorescent plate reading system (BioAssay Systems, Hayward, CA, USA) with filter settings of 360 ± 20 nm for excitation and 460 ± 20 nm for emission.

Western blot analysis
Whole cell lysates were prepared and analyzed by Western blotting as described previously [32]. Proteins in cell lysates were separated by precast 8-20% SDS-polyacrylamide gel electrophoresis, and then electrophoretically transferred from the gel onto polyvinylidene difluoride membranes. After blocking, blots were incubated with anti-GRP78, anti-GRP94, antiphospho-PERK, anti-caspase-12, anti-caspase-7, anti-CHOP, anti-PARP, and anti-β-actin antibodies (Santa Cruz Biotechnology) and anti-eIF2α (Cell Signaling Technology, Danvers, MA, USA) in PBS within 0.1% Tween 20 for 1 h followed by two 15 min washes in PBS with 0.1% Tween 20. The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies for 60 min. Detection was performed with Western blotting reagent ECL (Amersham-GE Healthcare Life Sciences, Pittsburgh, PA, USA), and chemiluminescence was exposed by the Kodak X-Omat films.

Statistical analyses
Results are expressed as means ± SEM. For multiple comparisons, results were analyzed by ANOVA followed by Fisher's test. P<0.05 was considered statistically significant.

CONFLICTS OF INTEREST
The authors declare no competing financial interest.

GRANT SUPPORT
This work was supported by grants from the Ministry of Science and Technology of Taiwan (MOST104-2314-B-002-126-MY3).