human
***task***
the task is to summarize an input biomedical literature in six sentences

***input***
the input is a biomedical literature

***output***
the output is the summary of an input biomedical literature in six sentences

***documentation***

***examples***

input: fish consumption reduces the risk of developing cardiovascular disease ( cvd ) and cvd mortality . reduced total mortality and major coronary events , including fatal and non - fatal mi , are observed in intervention trials after intake of fish and fish oil containing the marine n - 3 fatty acids eicosapentaenoic acid ( epa , 20:5 n - 3 ) and docosahexaenoic acid ( dha , 22:6 n - 3 ) . the association between high intakes of marine n - 3 fatty acids and decreased morbidity and mortality from cvd can be explained by the decrease in plasma triglycerides , moderate reduction in blood pressure and reduced blood plate aggregation . protection against cardiac arrhythmias has also been shown but the effect is still under discussion . , the combined action of risk factors causes the gradual thickening of the arterial wall due to lipid accumulation to form an atherosclerotic plaque which can abruptly rupture , causing thrombosis . there is an association between various biomarkers of inflammation and prospective cvd risk in apparently healthy individuals as well as in patients with cvd or heart failure . systemic biomarkers of early and late atherosclerosis are of great clinical interest due to their potential for identifying high risk patients . even though there are some good candidates , only c - reactive protein ( crp ) has emerged as a leading biomarker of inflammation for clinical application ; other markers with appropriate robustness , sensitivity and specificity have not yet emerged . based on the fact that endothelial dysfunction is the earliest manifestation of atherosclerosis , along with the involvement of oxidative stress and inflammation at all stages of coronary atherosclerosis development , biomarkers such as soluble intracellular adhesion molecule ( sicam - 1 ) and soluble vascular adhesion molecule ( svcam - 1 ) , soluble e - selectin ( se - sel ) ( for endothelial activation ) , interleukin ( il ) - 6 ( transcriptional driver of crp ) and monocyte chemoattractant protein ( mcp ) - 1 ( produced by activated vasculature ) are of particular interest ( table 1 ) . so far crp , il - 6 , svcam - 1 and sicam - 1 provide prognostic information beyond that obtained by clinical variables after acute coronary syndromes ; these mediators seem to be powerful predictors of subsequent cardiovascular events . recently , intake of marine n - 3 fatty acids has been associated with reduced plasma levels of inflammatory markers . table 1relevant inflammatory markers and their biological functioninflammatory markersabbreviationfunctionacute - phase protein c - reactive proteincrpcrp is associated with the formation of cytokines , chemokines and the acute - phase responsecytokines interleukin - 6il - 6induces acute - phase response ( by inducing crp ) , anti - body secretion and differentiation interleukin - 1 , interleukin - 1il - 1 , il - 1proliferation and maturation of lymphocytes , involved in inflammation and acute - phase response interleukin - 18il - 18involved in the formation of th1 cellstumor necrosis factor - is a cytokinetnf - induces adhesion molecules - and cytokine expression , involved in cell deathadhesion protein soluble intercellular adhesion molecule - 1sicam - 1binds monocytes and lymphocytes to the endothelium soluble vascular cell adhesion molecule - 1svcam - 1binds monocytes and lymphocytes to the endothelium se - selectinse - selrecruits leukocytes to the inflammatory site sp - selectinsp - selrecruits leukocytes to the inflammatory site . induces monocytes and platelet interactionschemokines monocyte chemoattractant protein - 1mcp - 1facilitates migration of leukocytes to the intima granulocyte macrophage colony - stimulating factorgmcsfgrowth and differentiation of monocytes interleukin - 8il - 8facilitates migration of leukocytes to the intima relevant inflammatory markers and their biological function the aim of the present paper was to review the literature in order to summarize the effects of marine n - 3 fatty acids on circulating inflammatory markers among healthy subjects , subjects with high risk of developing cvd and in patients with cvd in human intervention studies . the systematic literature search was conducted in pubmed in 2009 using the following terms : eicosapentaenoic acid or docosapentaenoic acid or docosahexaenoic acid or omega - 3 or fish oil or cod liver oil and inflammation . the following limitations were included in the search : added to pubmed in the last 10 years , published in the last 10 years , humans , clinical trial , english . in total , 91 articles were identified . based on these papers , we included all studies that measured the effect of marine n - 3 fatty acids on circulating inflammatory markers in plasma or serum among apparently healthy individuals ( table 2 ) , individuals with high risk of developing cvd ( table 3 ) or individuals with cvd ( table 4 ) . interventions with marine n - 3 fatty acids given as supplements or in the diet as fish were included . the following exclusion criteria were used : ( 1 ) interventions assessing inflammatory markers with ex vivo methods ( 2 ) , interventions with children ( 3 ) and articles describing animal or cell culture studies . after reading abstracts of the 91 papers , the literature lists of the selected papers were checked and 13 additional relevant papers were included based on the same inclusion and exclusion criteria as the literature search . however , no further papers were included from this search.table 2a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in healthy individualsstudyindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements geelen et al .43 men and 41 women , healthy ( 5070 years ) 2 groups : sunflower oil or fish oilfish oil : 0.7 g epa0 .56 g dha0 .26 g other n - 3 pufa12 weeks vega - lopez et al .80 healthy men and women ( 2055 years ) 4 groups : placebo , n - 3 pufa , vit e or n - 3 pufa / vit en - 3 pufa : 0.6 g epa0 .9 g dha1260 healthy men and women ( 2157 years ) 3 groups : olive oil , low dose fish oil or high dose fish oillow dose fish oil : 0.9 g epa0 .8 g dhahigh dose fish oil : 3 g epa2 .9 g dha12 weeks yusof et al .20 healthy men ( 3560 years ) 2 groups : coconut oil or fish oilfish oil : 1.8 g epa0 .3 g dha8 weeks il - 6 sicam - 1 svcam - 1 , se - sel , sp - sel fujioka et al .59 men and 82 women , healthy ( mean age 38 years ) 2 groups : olive oil or fish oilfish oil : 0.6 g epa0 .26 g dha12 weeks tnf - r1 , tnf - r2 ciubotaru et al .30 healthy women , postmenopausal and hormone treated ( mean age 60 years ) 3 groups : sunflower oil , low dose fish oil or high dose fish oillow dose fish oil : 1.3 g n - 3 pufahigh dose fish oil : 2.56 g n - 3 pufa5 weeks * il - 6 * pot et al .77 healthy elderly men and women ( 5070 years ) 2 groups : sunflower oil or fish oilfish oil : 0.7 g epa0 .56 g dha0 .26 g other n - 3 pufa12 weeks ( il - 1 , il - 1b , il - 2 , il - 4 , il - 5 , il - 6 , il - 10 , il - 12 , il - 13 , tnf - , ifn - ) mif , mcp - 1 , mip - 1 , rantes , ccl11 , il - 8 svcam - 1 and sicam - 1 thies et al .46 healthy men and women ( 5575 years ) 6 groups : palm and sunflower oils , ala , gla , aa , dha or fish oilfish oil : 0.72 g epa0 .28 g dha or dha 0.7 g12 weeks s - vcam - 1 ( fish oil and ala ) se - sel ( fish oil and ala ) sicam - 1 miles et al .16 men 40 years and 12 elderly men and women 55 years2 groups : palm oil and soy bean oils or fish oilfish oil : 1.2 g epa / dha12 weeks sicam - 1 svcam - 1 ( older ) se - sel ( young ) michaeli et al .15 healthy men ( mean age 26 years ) 2 groups : fish oil or not ( not - blinded ) fish oil : 1.1 g epa0 .7 g dha34 weeks followed by lps stimulation tnf - , il - 6 cazolla et al .93 healthy young men ( 1842 years ) and 62 healthy elderly men ( 5370 years ) 4 groups : corn oil or 3 different doses epa oilepa oil ( epax 4510tg ) : 1.35 g , 2.7 g or 4.05 g epa12 weeks se - sel ( 4,05 g epa , young men ) sicam - 1 ( 4,05 g epa , both groups ) ( tendency ) interventions with n - 3 diet tsitouras et al .12 healthy elderly men and women ( 6075 years ) 2 groups : control diet or n - 3 diet ( cross - over design ) n - 3 diet : 720 g / week fatty fish and sardine oil ( 45 g / day epa and dha ) 8 weeks il - 6 ( tendency ) paulo mc 275 healthy men and women ( 2040 years ) 4 groups : sunflower oil , fish oil , lean fish or fatty fishfish oil : 0.63 g epa and 0.43 g dhalean fish : 0.05 g epa and 0.21 g dhafatty fish : 0.77 g epa and 1.37 g dha8 weeks sicam - 1 ( lean fish ) svcam - 1 ( fish oil and lean fish ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated * larger change in crp and il - 6 with fish oil with low content of epa and dha compared to fish oil with high contentala - linolenic acid ( c18 ; 3 , n - 3 ) , gla - linolenic acid ( c18 ; 3 , n - 6 ) , aa arachidonic acid ( c20 : 4 , n - 6 ) table 3a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with high cvd riskstudyindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements kelley et al . 34 hyperlipidemic men ( 3966 years ) 2 groups : olive oil or dha - oildha - oil : 3 g dha3 months il - 6 il - 1 , il - 2 , il - 10 , tnf - gm - csf circulating neutrophilsmmp - 2 il - 8 murphy et al. 74 overweight individuals , bmi 25 and tg 1.6 , ( 2065 years ) 2 groups : n - 3 enriched diet or no enriched dietn - 3 enriched food : 1 g epa and dha6 months plat et al .11 healthy overweight , bmi : 30 - 35 , ( mean age 59 years ) 2 groups : sunflower oil or fish oil ( cross - over design ) fish oil : 0.6 g epa0 .5 g dha6 weeks sicam - 1 , se - sel , mcp - 1 browning et al. 30 healthy overweight women , bmi 252 groups : placebo ( la and oa ) or fish oil ( cross - over design ) fish oil : 1.3 g epa2 .9 g dha12 weeks il - 6 kabir et al .26 women with type 2 diabetes , 4060 years2 groups : placebo ( paraffin oil ) or fish oilfish oil : 1.08 g epa0 .72 g dha2 months il - 6 , tnf - krebs et al .93 overweight women , bmi 27 , fasting insulin 7 , ( 2169 years ) 3 groups : placebo ( la and oa ) , fish oil + weight reduction or placebo + weight reductionfish oil : 1.3 g epa2 .9 g dha24 weeks il - 6 , tnf - jellema et al .11 overweight men , bmi : 30 - 352 groups : sunflower oil or fish oil ( cross - over design ) fish oil : 1.35 g epa and dha6 weeks il - 6 , tnf - ( fasting or postprandial ) stnf - r55 , stnf - r75 ( fasting or postprandial ) mori et al .59 hypertensive type 2 diabetic patients ( 4075 years ) 3 groups : olive oil , epa or dhan - 3 pufa : 4 g epa ( ethyl ester ) or4 g dha ( ethyl ester ) 6 weeks il - 6 , tnf - chan et al . 48 overweight men , bmi 29 , mean age 53 years4 groups : corn oil , atorvastatin + corn oil , fish oil , atorvastatin + fish oiln - 3 pufa : omacore 4 g / day ( 1.8 g epa and 1.56 g dha ethyl ester ) 6 weeks il - 6 , tnf - accinni et al .57 dyslipidemic individuals ( 2365 years ) 3 groups : placebo , n - 3 pufa / vite or n - 3 pufa / vite and - oryzanol / niacinn - 3 pufa : 0.66 g epa0 .4429 individuals with type 2diabetes and 21 healthy controls4 groups : controls and patients were given n - 3 pufa or not . ( not placebo controlled ) n - 3 pufa : 1.2 g epa0 .8 g dha3 weeks svcam - 1 , sicam - 1 , se - sel , seljeflot et al . 41 male smokers with hyperlipidemia ( 4557 years ) 4 groups : n - 3 pufa and antioxidatns , n - 3 pufa , antioxidants , placebon - 3 pufa : 4.8 g epa and dha ( ethyl ester ) 6 weeks se - sel , svcam - 1 ( n - 3 pufa ) interventions with n - 3 diet troseid et al .487 elderly men ( 6476 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables3 years il - 18 ( dietary intervention , n - 3 pufa ) il - 6 , tnf - , mcp - 1 hjerkinn et al .487 elderly men with high cvd risk ( 6476 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables3 years sicam - 1 ( n - 3 pufa , dietary intervention , dietary intervention + n - 3 pufa ) berstad 171 elderly men with high cvd risk ( 6575 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables18 months sicam - 1 , svcam - 1 # se - sel ( dietary intervention ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated positive correlation between change in serum n - 3 pufa and svcam - 1tg triglycerides , oa oleic acid ( c18 : 1 , n - 9 ) , la linoleic acid ( c18 : 2 , n - 6 ) table 4a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with cvd and cvd related diseasesindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements schiano et al .32 men and women with pad , mean age 66 years2 groups : no change in treatment or n - 3 pufa ( not blinded ) n - 3 pufa : 0.85 g epa and dha ( ethyl ester ) 12 weeks myeloperoxidase madsen et al .41 men and women with previous mi , mean age 63 years2 groups : clive oil or n - 3252 men and women with previous mi , ( 2887 years ) 2 groups : corn oil or n - 3 pufan - 3 pufa : 3.4 g epa and dha ( ethyl ester ) 12 months sicam - 1 , se - sel lee et al .77 men and women with previous mi , 40 healthy controls , mean age 57 years2 groups : no change in treatment or n - 3 pufa ( not blinded ) n - 3 pufa : omacore 1 g / day ( ethyl ester ) 12 weeks il - 6 sp - sel thies et al .162 men and women awaiting carotid endarterectomy , mean age 69 years3 groups : palm and soy bean oils , sunflower oil or fish oilfish oil : 1.4 g n - 3 pufa7189 days ( median 42 days ) sicam - 1 , svcam - 1 in plaques macrophage infiltration ( anti - cd68 ) in plaques ( fish oil ) johansen et al .54 men and women with chd , mean age 58 years2 groups : corn oil or n - 3 pufa 5,1 g for 6 months , followed by 4 weeks where both groups received n - 3 pufan - 3 pufa : 5.1 g epa and dha4 weeks svcam - 1 , se sel ( corn oil followed by 4 weeks with n - 3 pufa ) interventions with n - 3 diet seierstad et al .60 men and women with chd ( 4675 years ) 3 groups : all groups got 700 g salmon / week with low , moderate or high content of n - 3 pufan - 3 content in salmon : low n - 3 : 0.5 g epa + dhamoderate n - 3 : 1.5 g epa + dhahigh n - 3 : 2.9 g epa + dha6 weeks il - 6 , ( high n - 3 ) svcam - 1 ( high n - 3 ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise statedmi myocardial infarction , pad peripheral arterial disease , chd coronary heart disease a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in healthy individuals all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated * larger change in crp and il - 6 with fish oil with low content of epa and dha compared to fish oil with high content ala - linolenic acid ( c18 ; 3 , n - 3 ) , gla - linolenic acid ( c18 ; 3 , n - 6 ) , aa arachidonic acid ( c20 : 4 , n - 6 ) a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with high cvd risk all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated positive correlation between change in serum n - 3 pufa and svcam - 1 tg triglycerides , oa oleic acid ( c18 : 1 , n - 9 ) , la linoleic acid ( c18 : 2 , n - 6 ) a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with cvd and cvd related diseases all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated mi myocardial infarction , pad peripheral arterial disease , chd coronary heart diseasedetails of the 35 studies selected in this review are given in tables 2 , 3 and 4 . twenty - nine of these are based on marine n - 3 fatty acids given as supplements , whereas six studies are dietary interventions with fish . the systematic review of the literature reveals that seven out of thirteen trials reported an effect on circulating inflammatory markers in healthy individuals ( table 2 ) . six of the studies did not observe any changes in inflammatory markers ( table 2 ) . c - reactive protein ( crp ) was analyzed in seven of the trials , and in five of these no effect in crp levels was observed , while a reduction in plasma concentration of crp after intake of marine n - 3 fatty acids was reported in two of the trials . two of these reported a reduction in il - 6 levels , whereas three did not demonstrate any effect on il - 6 levels . table 2 also reveals that marine n - 3 fatty acids reduced or had no effects on the concentration of sicam - 1 in five of the trials . the concentration of svcam - 1 was reduced , increased or unchanged after intake of marine n - 3 fatty acids among healthy individuals in these trials , whereas the level of se - sel increased or remained unchanged . the effects of marine n - 3 fatty acids on circulating inflammatory markers among individuals with a high risk of developing cvd are shown in table 3 . four of fifteen trials reported a reduction in the concentration of inflammatory markers after intake of marine n - 3 fatty acids , while nine studies did not observe any effects on inflammation . one of nine studies where crp was analyzed demonstrated a reduction in the concentration of crp after intake of marine n - 3 fatty acids . three trials demonstrated a reduction in plasma cytokines or soluble adhesion molecules . table 4 reviews the effects of marine n - 3 fatty acids on circulating inflammatory markers among individuals with cvd or cvd related diseases . five of seven studies showed no change in the concentration of inflammatory markers after intake of marine n - 3 fatty acids , whereas one study reported a reduction in the levels of il - 6 and svcam - 1 and one trial showed an increase in the level of se - sel and svcam -1 . intervention trials with marine n - 3 fatty acids administered either from fish or fish oil supplements demonstrate different results on circulating inflammatory markers in healthy individuals , individuals with high risk of developing cvd or individuals with cvd related diseases . based on these findings , a firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can not be drawn . the majority of the trials do not show any effect , however a decreased level of circulating crp and il - 6 are observed in some studies , whereas the circulating level of adhesion molecules inconsistently are elevated , decreased or not changed . first of all , a local inflammatory improvement in the arterial wall will not necessary be reflected in the circulation and may explain the lack of effect observed after intake of marine n - 3 fatty acids . several of the reported trials have a relative small number of study subjects and probably low statistical power . variation in the results can also be explained by differences in the administration of the marine n - 3 fatty acids ( supplement vs. diet ) as well as the doses used . furthermore , there is uncertainty regarding the background diet and to what extend the intervention changed the balance between n - 3 and n - 6 fatty acids or altered the intake of saturated fat in the diet . comparing the results on the effects of marine n - 3 fatty acids given as supplements on serum crp in healthy individuals , the baseline level of crp seems crucial . reported a significant reduction in the level of crp in postmenopausal women who had mean baseline levels of crp above 6 mg / l . , vega - lopez et al . and madsen et al . who did not observe any changes in the crp serum levels . the baseline level of serum crp in the subjects in these studies was approximately 1vega - lopez et al . failed to see any effect on the serum crp level using a daily dose of 0.6 g epa and 0.9 dha for 12 weeks , which is in line with the results from madsen et al . who used two different daily doses ( 3.0 g epa + 2.9 g dha or 0.9 g epa + 0.8 g dha ) . in contrast , ciubotaru et al . observed in postmenopausal women that the effect on crp is dependent on the dose of marine n - 3 fatty acids . a larger decrease in crpwas observed in the women who received the highest dose of marine n - 3 fatty acids ( 2.56 g / d ) compared to those receiving a daily dose of 1.3 g marine n - 3 fatty acids . it is interesting to note that observed decrease in circulating crp level was associated with decrease in circulating il - 6 level after intake of marine n - 3 fatty acids , both via supplement or diet . in this reviewthere are relatively few studies where marine n - 3 fatty acids are administered as fish . when comparing these interventions with the interventions with fish oil , there are indications for a more prominent effect on circulating inflammatory markers with fish containing diet . reductions or no change in one of the following markers ( crp , il - 6 , sicam - 1 , svcam - 1 , il - 18 , se - sel ) are observed in all the six dietary studies included in this review . a possible explanation could be that subjects participating in dietary intervention trials may change their overall dietary pattern resulting in more awareness of healthy lifestyle , which again may influence the circulating inflammatory markers . fish may also contain other bioactive compounds that may influence outcome . to further understand if marine n - 3 fatty acids have an effect on inflammation in relation to atherosclerosis and the underlying molecular mechanisms responsible for their inflammatory response , new strategies should be considered . when inflammatory markers are measured in the circulation , the local effect in monocytes will not necessary be detected since the monocytes only contribute to about 5 % of the total cells in plasma . the peripheral blood mononuclear cells ( pbmcs ) include monocytes and lymphocytes , which are cells central in inflammation and hence in the atherosclerosis process . the pbmcs are exposed to many of the same environmental factors as the arterial wall . alterations in gene expression levels in these cells can thus be demonstrated early in the process before signs of inflammation can be seen in vivo , and thus constitute an important source of biomarkers for progression of atherosclerotic disease . pbmcs are easily available , which makes them suitable for studying gene expression of mediators involved in the early development of atherosclerosis . a previous study recently demonstrated that 166 genes among 182 candidate cardiovascular genes were expressed in pbmcs . the ability of marine n - 3 fatty acids to alter gene expression has been clearly demonstrated in vitro and may account for its potential beneficial health effects . fatty acids regulate expression of genes involved in lipid metabolism and inflammation by acting as ligands for the peroxisomal proliferator - activated receptors ( ppars ) . fatty acids are also known to reduce the activation of the transcription factor nuclear factor kappa b ( nf - b ) probably by interference with the ppars or the toll - like receptors . the g protein - coupled receptor 120 ( gpr120 ) has recently been characterized as an n - 3 fatty acid receptor / sensor involved in the anti - inflammatory effects of n - 3 fatty acids . dietary supplements containing fish oil have also been shown to affect expression of pi3k , akt , nf - b and inflammatory cytokines in mononuclear cells . to obtain a more comprehensive overview of the processes that are modulated by epa and dha , newomics technologies can be used to detect large scale changes in gene expression profiles ( transcriptomics ) . whole genome transcriptomic analysis in pbmcswill be valuable in further understanding the inflammatory role of n - 3 fatty acids in humans . recently , a human intervention study among elderly who daily consumed fish oil ( containing 1.8 g epa and dha ) for 26 weeks demonstrated changes in the gene expression profiles in pbmcs . gene transcripts involved in inflammation and other processes of atherosclerosis were down - regulated among those who had consumed fish oil . the combined use of other omics technologies , such as proteomics and metabolomics ( lipidomics ) , in intervention studieswill further extend the understanding of the effects of marine n - 3 fatty acids on inflammation . using proteomics technology , a recent study demonstrated that daily intake of 3.5 g fish oil down - regulated a number of proteins involved in the acute phase response and lipid / lipoprotein metabolism . furthermore , lankinen et al . previously used a lipidomics approach in an intervention study where 33 individuals randomized to eat fatty fish ( 150 g four times per week ) , lean fish ( 150 g four times per week ) or no fish , in 8 weeks . they observed a reduction in the level of lipid metabolites related to inflammation and insulin - signaling among those eating fatty fish . no firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can be drawn in this review . a systematic use of transcriptome , proteome , and metabolome technologies for the construction of network based models of biological processes has emerged as an exciting research approach in molecular biology and functional genomics . in futuremarine n - 3 supplementation and dietary intervention trials , systems biology combined with traditional circulating markers may help us to gain more insight into the underlying molecular mechanisms of marine n - 3 fatty acids responsible for the inflammatory response in the progression of atherosclerotic disease . intervention trials with marine n - 3 fatty acids administered either from fish or fish oil supplements demonstrate different results on circulating inflammatory markers in healthy individuals , individuals with high risk of developing cvd or individuals with cvd related diseases . based on these findings , a firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can not be drawn . the majority of the trials do not show any effect , however a decreased level of circulating crp and il - 6 are observed in some studies , whereas the circulating level of adhesion molecules inconsistently are elevated , decreased or not changed . first of all , a local inflammatory improvement in the arterial wall will not necessary be reflected in the circulation and may explain the lack of effect observed after intake of marine n - 3 fatty acids . several of the reported trials have a relative small number of study subjects and probably low statistical power . variation in the results can also be explained by differences in the administration of the marine n - 3 fatty acids ( supplement vs. diet ) as well as the doses used . furthermore , there is uncertainty regarding the background diet and to what extend the intervention changed the balance between n - 3 and n - 6 fatty acids or altered the intake of saturated fat in the diet . comparing the results on the effects of marine n - 3 fatty acids given as supplements on serum crp in healthy individuals , the baseline level of crp seems crucial . reported a significant reduction in the level of crp in postmenopausal women who had mean baseline levels of crp above 6 mg / l . , vega - lopez et al . and madsen et al . who did not observe any changes in the crp serum levels . the baseline level of serum crp in the subjects in these studies was approximately 1vega - lopez et al . failed to see any effect on the serum crp level using a daily dose of 0.6 g epa and 0.9 dha for 12 weeks , which is in line with the results from madsen et al . who used two different daily doses ( 3.0 g epa + 2.9 g dha or 0.9 g epa + 0.8 g dha ) . in contrast , ciubotaru et al . observed in postmenopausal women that the effect on crp is dependent on the dose of marine n - 3 fatty acids . a larger decrease in crpwas observed in the women who received the highest dose of marine n - 3 fatty acids ( 2.56 g / d ) compared to those receiving a daily dose of 1.3 g marine n - 3 fatty acids . it is interesting to note that observed decrease in circulating crp level was associated with decrease in circulating il - 6 level after intake of marine n - 3 fatty acids , both via supplement or diet . in this reviewthere are relatively few studies where marine n - 3 fatty acids are administered as fish . when comparing these interventions with the interventions with fish oil , there are indications for a more prominent effect on circulating inflammatory markers with fish containing diet . reductions or no change in one of the following markers ( crp , il - 6 , sicam - 1 , svcam - 1 , il - 18 , se - sel ) are observed in all the six dietary studies included in this review . a possible explanation could be that subjects participating in dietary intervention trials may change their overall dietary pattern resulting in more awareness of healthy lifestyle , which again may influence the circulating inflammatory markers . to further understand if marine n - 3 fatty acids have an effect on inflammation in relation to atherosclerosis and the underlying molecular mechanisms responsible for their inflammatory response , new strategies should be considered . when inflammatory markers are measured in the circulation , the local effect in monocytes will not necessary be detected since the monocytes only contribute to about 5 % of the total cells in plasma . the peripheral blood mononuclear cells ( pbmcs ) include monocytes and lymphocytes , which are cells central in inflammation and hence in the atherosclerosis process . the pbmcs are exposed to many of the same environmental factors as the arterial wall . alterations in gene expression levels in these cells can thus be demonstrated early in the process before signs of inflammation can be seen in vivo , and thus constitute an important source of biomarkers for progression of atherosclerotic disease . pbmcs are easily available , which makes them suitable for studying gene expression of mediators involved in the early development of atherosclerosis . a previous study recently demonstrated that 166 genes among 182 candidate cardiovascular genes were expressed in pbmcs . the ability of marine n - 3 fatty acids to alter gene expression has been clearly demonstrated in vitro and may account for its potential beneficial health effects . fatty acids regulate expression of genes involved in lipid metabolism and inflammation by acting as ligands for the peroxisomal proliferator - activated receptors ( ppars ) . fatty acids are also known to reduce the activation of the transcription factor nuclear factor kappa b ( nf - b ) probably by interference with the ppars or the toll - like receptors . the g protein - coupled receptor 120 ( gpr120 ) has recently been characterized as an n - 3 fatty acid receptor / sensor involved in the anti - inflammatory effects of n - 3 fatty acids . dietary supplements containing fish oil have also been shown to affect expression of pi3k , akt , nf - b and inflammatory cytokines in mononuclear cells . to obtain a more comprehensive overview of the processes that are modulated by epa and dha , newomics technologies can be used to detect large scale changes in gene expression profiles ( transcriptomics ) . whole genome transcriptomic analysis in pbmcswill be valuable in further understanding the inflammatory role of n - 3 fatty acids in humans . recently , a human intervention study among elderly who daily consumed fish oil ( containing 1.8 g epa and dha ) for 26 weeks demonstrated changes in the gene expression profiles in pbmcs . gene transcripts involved in inflammation and other processes of atherosclerosis were down - regulated among those who had consumed fish oil . the combined use of other omics technologies , such as proteomics and metabolomics ( lipidomics ) , in intervention studieswill further extend the understanding of the effects of marine n - 3 fatty acids on inflammation . using proteomics technology , a recent study demonstrated that daily intake of 3.5 g fish oil down - regulated a number of proteins involved in the acute phase response and lipid / lipoprotein metabolism . previously used a lipidomics approach in an intervention study where 33 individuals randomized to eat fatty fish ( 150 g four times per week ) , lean fish ( 150 g four times per week ) or no fish , in 8 weeks . they observed a reduction in the level of lipid metabolites related to inflammation and insulin - signaling among those eating fatty fish . no firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can be drawn in this review . a systematic use of transcriptome , proteome , and metabolome technologies for the construction of network based models of biological processes has emerged as an exciting research approach in molecular biology and functional genomics . in future marine n - 3 supplementation and dietary intervention trials , systems biology combined with traditional circulating markers may help us to gain more insight into the underlying molecular mechanisms of marine n - 3 fatty acids responsible for the inflammatory response in the progression of atherosclerotic disease .
output:

agent
objectivethe aim of the present paper was to review the literature in order to summarize the effects of marine n - 3 fatty acids on circulating inflammatory markers among healthy subjects , subjects with high risk of developing cardiovascular disease ( cvd ) and in patients with cvd in human intervention studies.methodsa systematic literature search in pubmed was performed . intervention studies describing the effects of marine n - 3 fatty acids on circulating inflammatory markers in healthy subjects , subjects with high risk of cvd and patients with cvd were included . the following exclusion criteria were used : ( 1 ) interventions assessing inflammatory markers with ex vivo methods ( 2 ) interventions with children ( 3 ) articles describing animal or cell culture studies . twenty - two articles were included . additionally , 13 papers from their literature lists were included based on the same inclusion and exclusion criteria as the literature search.results and conclusionintervention studies with marine n - 3 fatty acids administered from either fish or fish oil demonstrate different results on inflammatory markers . no firm conclusion can be drawn about the effect of marine n - 3 fatty acids on circulating inflammatory markers in healthy individuals , individuals with high risk of developing cvd or individuals with cvd related diseases .
***task***
the task is to summarize an input biomedical literature in six sentences

***input***
the input is a biomedical literature

***output***
the output is the summary of an input biomedical literature in six sentences

***documentation***

***examples***

input: fish consumption reduces the risk of developing cardiovascular disease ( cvd ) and cvd mortality . reduced total mortality and major coronary events , including fatal and non - fatal mi , are observed in intervention trials after intake of fish and fish oil containing the marine n - 3 fatty acids eicosapentaenoic acid ( epa , 20:5 n - 3 ) and docosahexaenoic acid ( dha , 22:6 n - 3 ) . the association between high intakes of marine n - 3 fatty acids and decreased morbidity and mortality from cvd can be explained by the decrease in plasma triglycerides , moderate reduction in blood pressure and reduced blood plate aggregation . protection against cardiac arrhythmias has also been shown but the effect is still under discussion . , the combined action of risk factors causes the gradual thickening of the arterial wall due to lipid accumulation to form an atherosclerotic plaque which can abruptly rupture , causing thrombosis . there is an association between various biomarkers of inflammation and prospective cvd risk in apparently healthy individuals as well as in patients with cvd or heart failure . systemic biomarkers of early and late atherosclerosis are of great clinical interest due to their potential for identifying high risk patients . even though there are some good candidates , only c - reactive protein ( crp ) has emerged as a leading biomarker of inflammation for clinical application ; other markers with appropriate robustness , sensitivity and specificity have not yet emerged . based on the fact that endothelial dysfunction is the earliest manifestation of atherosclerosis , along with the involvement of oxidative stress and inflammation at all stages of coronary atherosclerosis development , biomarkers such as soluble intracellular adhesion molecule ( sicam - 1 ) and soluble vascular adhesion molecule ( svcam - 1 ) , soluble e - selectin ( se - sel ) ( for endothelial activation ) , interleukin ( il ) - 6 ( transcriptional driver of crp ) and monocyte chemoattractant protein ( mcp ) - 1 ( produced by activated vasculature ) are of particular interest ( table 1 ) . so far crp , il - 6 , svcam - 1 and sicam - 1 provide prognostic information beyond that obtained by clinical variables after acute coronary syndromes ; these mediators seem to be powerful predictors of subsequent cardiovascular events . recently , intake of marine n - 3 fatty acids has been associated with reduced plasma levels of inflammatory markers . table 1relevant inflammatory markers and their biological functioninflammatory markersabbreviationfunctionacute - phase protein c - reactive proteincrpcrp is associated with the formation of cytokines , chemokines and the acute - phase responsecytokines interleukin - 6il - 6induces acute - phase response ( by inducing crp ) , anti - body secretion and differentiation interleukin - 1 , interleukin - 1il - 1 , il - 1proliferation and maturation of lymphocytes , involved in inflammation and acute - phase response interleukin - 18il - 18involved in the formation of th1 cellstumor necrosis factor - is a cytokinetnf - induces adhesion molecules - and cytokine expression , involved in cell deathadhesion protein soluble intercellular adhesion molecule - 1sicam - 1binds monocytes and lymphocytes to the endothelium soluble vascular cell adhesion molecule - 1svcam - 1binds monocytes and lymphocytes to the endothelium se - selectinse - selrecruits leukocytes to the inflammatory site sp - selectinsp - selrecruits leukocytes to the inflammatory site . induces monocytes and platelet interactionschemokines monocyte chemoattractant protein - 1mcp - 1facilitates migration of leukocytes to the intima granulocyte macrophage colony - stimulating factorgmcsfgrowth and differentiation of monocytes interleukin - 8il - 8facilitates migration of leukocytes to the intima relevant inflammatory markers and their biological function the aim of the present paper was to review the literature in order to summarize the effects of marine n - 3 fatty acids on circulating inflammatory markers among healthy subjects , subjects with high risk of developing cvd and in patients with cvd in human intervention studies . the systematic literature search was conducted in pubmed in 2009 using the following terms : eicosapentaenoic acid or docosapentaenoic acid or docosahexaenoic acid or omega - 3 or fish oil or cod liver oil and inflammation . the following limitations were included in the search : added to pubmed in the last 10 years , published in the last 10 years , humans , clinical trial , english . in total , 91 articles were identified . based on these papers , we included all studies that measured the effect of marine n - 3 fatty acids on circulating inflammatory markers in plasma or serum among apparently healthy individuals ( table 2 ) , individuals with high risk of developing cvd ( table 3 ) or individuals with cvd ( table 4 ) . interventions with marine n - 3 fatty acids given as supplements or in the diet as fish were included . the following exclusion criteria were used : ( 1 ) interventions assessing inflammatory markers with ex vivo methods ( 2 ) , interventions with children ( 3 ) and articles describing animal or cell culture studies . after reading abstracts of the 91 papers , the literature lists of the selected papers were checked and 13 additional relevant papers were included based on the same inclusion and exclusion criteria as the literature search . however , no further papers were included from this search.table 2a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in healthy individualsstudyindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements geelen et al .43 men and 41 women , healthy ( 5070 years ) 2 groups : sunflower oil or fish oilfish oil : 0.7 g epa0 .56 g dha0 .26 g other n - 3 pufa12 weeks vega - lopez et al .80 healthy men and women ( 2055 years ) 4 groups : placebo , n - 3 pufa , vit e or n - 3 pufa / vit en - 3 pufa : 0.6 g epa0 .9 g dha1260 healthy men and women ( 2157 years ) 3 groups : olive oil , low dose fish oil or high dose fish oillow dose fish oil : 0.9 g epa0 .8 g dhahigh dose fish oil : 3 g epa2 .9 g dha12 weeks yusof et al .20 healthy men ( 3560 years ) 2 groups : coconut oil or fish oilfish oil : 1.8 g epa0 .3 g dha8 weeks il - 6 sicam - 1 svcam - 1 , se - sel , sp - sel fujioka et al .59 men and 82 women , healthy ( mean age 38 years ) 2 groups : olive oil or fish oilfish oil : 0.6 g epa0 .26 g dha12 weeks tnf - r1 , tnf - r2 ciubotaru et al .30 healthy women , postmenopausal and hormone treated ( mean age 60 years ) 3 groups : sunflower oil , low dose fish oil or high dose fish oillow dose fish oil : 1.3 g n - 3 pufahigh dose fish oil : 2.56 g n - 3 pufa5 weeks * il - 6 * pot et al .77 healthy elderly men and women ( 5070 years ) 2 groups : sunflower oil or fish oilfish oil : 0.7 g epa0 .56 g dha0 .26 g other n - 3 pufa12 weeks ( il - 1 , il - 1b , il - 2 , il - 4 , il - 5 , il - 6 , il - 10 , il - 12 , il - 13 , tnf - , ifn - ) mif , mcp - 1 , mip - 1 , rantes , ccl11 , il - 8 svcam - 1 and sicam - 1 thies et al .46 healthy men and women ( 5575 years ) 6 groups : palm and sunflower oils , ala , gla , aa , dha or fish oilfish oil : 0.72 g epa0 .28 g dha or dha 0.7 g12 weeks s - vcam - 1 ( fish oil and ala ) se - sel ( fish oil and ala ) sicam - 1 miles et al .16 men 40 years and 12 elderly men and women 55 years2 groups : palm oil and soy bean oils or fish oilfish oil : 1.2 g epa / dha12 weeks sicam - 1 svcam - 1 ( older ) se - sel ( young ) michaeli et al .15 healthy men ( mean age 26 years ) 2 groups : fish oil or not ( not - blinded ) fish oil : 1.1 g epa0 .7 g dha34 weeks followed by lps stimulation tnf - , il - 6 cazolla et al .93 healthy young men ( 1842 years ) and 62 healthy elderly men ( 5370 years ) 4 groups : corn oil or 3 different doses epa oilepa oil ( epax 4510tg ) : 1.35 g , 2.7 g or 4.05 g epa12 weeks se - sel ( 4,05 g epa , young men ) sicam - 1 ( 4,05 g epa , both groups ) ( tendency ) interventions with n - 3 diet tsitouras et al .12 healthy elderly men and women ( 6075 years ) 2 groups : control diet or n - 3 diet ( cross - over design ) n - 3 diet : 720 g / week fatty fish and sardine oil ( 45 g / day epa and dha ) 8 weeks il - 6 ( tendency ) paulo mc 275 healthy men and women ( 2040 years ) 4 groups : sunflower oil , fish oil , lean fish or fatty fishfish oil : 0.63 g epa and 0.43 g dhalean fish : 0.05 g epa and 0.21 g dhafatty fish : 0.77 g epa and 1.37 g dha8 weeks sicam - 1 ( lean fish ) svcam - 1 ( fish oil and lean fish ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated * larger change in crp and il - 6 with fish oil with low content of epa and dha compared to fish oil with high contentala - linolenic acid ( c18 ; 3 , n - 3 ) , gla - linolenic acid ( c18 ; 3 , n - 6 ) , aa arachidonic acid ( c20 : 4 , n - 6 ) table 3a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with high cvd riskstudyindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements kelley et al . 34 hyperlipidemic men ( 3966 years ) 2 groups : olive oil or dha - oildha - oil : 3 g dha3 months il - 6 il - 1 , il - 2 , il - 10 , tnf - gm - csf circulating neutrophilsmmp - 2 il - 8 murphy et al. 74 overweight individuals , bmi 25 and tg 1.6 , ( 2065 years ) 2 groups : n - 3 enriched diet or no enriched dietn - 3 enriched food : 1 g epa and dha6 months plat et al .11 healthy overweight , bmi : 30 - 35 , ( mean age 59 years ) 2 groups : sunflower oil or fish oil ( cross - over design ) fish oil : 0.6 g epa0 .5 g dha6 weeks sicam - 1 , se - sel , mcp - 1 browning et al. 30 healthy overweight women , bmi 252 groups : placebo ( la and oa ) or fish oil ( cross - over design ) fish oil : 1.3 g epa2 .9 g dha12 weeks il - 6 kabir et al .26 women with type 2 diabetes , 4060 years2 groups : placebo ( paraffin oil ) or fish oilfish oil : 1.08 g epa0 .72 g dha2 months il - 6 , tnf - krebs et al .93 overweight women , bmi 27 , fasting insulin 7 , ( 2169 years ) 3 groups : placebo ( la and oa ) , fish oil + weight reduction or placebo + weight reductionfish oil : 1.3 g epa2 .9 g dha24 weeks il - 6 , tnf - jellema et al .11 overweight men , bmi : 30 - 352 groups : sunflower oil or fish oil ( cross - over design ) fish oil : 1.35 g epa and dha6 weeks il - 6 , tnf - ( fasting or postprandial ) stnf - r55 , stnf - r75 ( fasting or postprandial ) mori et al .59 hypertensive type 2 diabetic patients ( 4075 years ) 3 groups : olive oil , epa or dhan - 3 pufa : 4 g epa ( ethyl ester ) or4 g dha ( ethyl ester ) 6 weeks il - 6 , tnf - chan et al . 48 overweight men , bmi 29 , mean age 53 years4 groups : corn oil , atorvastatin + corn oil , fish oil , atorvastatin + fish oiln - 3 pufa : omacore 4 g / day ( 1.8 g epa and 1.56 g dha ethyl ester ) 6 weeks il - 6 , tnf - accinni et al .57 dyslipidemic individuals ( 2365 years ) 3 groups : placebo , n - 3 pufa / vite or n - 3 pufa / vite and - oryzanol / niacinn - 3 pufa : 0.66 g epa0 .4429 individuals with type 2diabetes and 21 healthy controls4 groups : controls and patients were given n - 3 pufa or not . ( not placebo controlled ) n - 3 pufa : 1.2 g epa0 .8 g dha3 weeks svcam - 1 , sicam - 1 , se - sel , seljeflot et al . 41 male smokers with hyperlipidemia ( 4557 years ) 4 groups : n - 3 pufa and antioxidatns , n - 3 pufa , antioxidants , placebon - 3 pufa : 4.8 g epa and dha ( ethyl ester ) 6 weeks se - sel , svcam - 1 ( n - 3 pufa ) interventions with n - 3 diet troseid et al .487 elderly men ( 6476 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables3 years il - 18 ( dietary intervention , n - 3 pufa ) il - 6 , tnf - , mcp - 1 hjerkinn et al .487 elderly men with high cvd risk ( 6476 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables3 years sicam - 1 ( n - 3 pufa , dietary intervention , dietary intervention + n - 3 pufa ) berstad 171 elderly men with high cvd risk ( 6575 years ) 4 groups : corn oil , n - 3 pufa , corn oil + dietary intervention or n - 3 pufa + dietary interventionn - 3 pufa : 0.84 g epa0 .48 g dhadietary intervention : increase intake of unsaturated fat , fish , fruit and vegetables18 months sicam - 1 , svcam - 1 # se - sel ( dietary intervention ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated positive correlation between change in serum n - 3 pufa and svcam - 1tg triglycerides , oa oleic acid ( c18 : 1 , n - 9 ) , la linoleic acid ( c18 : 2 , n - 6 ) table 4a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with cvd and cvd related diseasesindividuals ( n ) number of groupsdose n - 3 ( g / day ) durationcrpcytokinesother inflammatory markersinterventions with n - 3 supplements schiano et al .32 men and women with pad , mean age 66 years2 groups : no change in treatment or n - 3 pufa ( not blinded ) n - 3 pufa : 0.85 g epa and dha ( ethyl ester ) 12 weeks myeloperoxidase madsen et al .41 men and women with previous mi , mean age 63 years2 groups : clive oil or n - 3252 men and women with previous mi , ( 2887 years ) 2 groups : corn oil or n - 3 pufan - 3 pufa : 3.4 g epa and dha ( ethyl ester ) 12 months sicam - 1 , se - sel lee et al .77 men and women with previous mi , 40 healthy controls , mean age 57 years2 groups : no change in treatment or n - 3 pufa ( not blinded ) n - 3 pufa : omacore 1 g / day ( ethyl ester ) 12 weeks il - 6 sp - sel thies et al .162 men and women awaiting carotid endarterectomy , mean age 69 years3 groups : palm and soy bean oils , sunflower oil or fish oilfish oil : 1.4 g n - 3 pufa7189 days ( median 42 days ) sicam - 1 , svcam - 1 in plaques macrophage infiltration ( anti - cd68 ) in plaques ( fish oil ) johansen et al .54 men and women with chd , mean age 58 years2 groups : corn oil or n - 3 pufa 5,1 g for 6 months , followed by 4 weeks where both groups received n - 3 pufan - 3 pufa : 5.1 g epa and dha4 weeks svcam - 1 , se sel ( corn oil followed by 4 weeks with n - 3 pufa ) interventions with n - 3 diet seierstad et al .60 men and women with chd ( 4675 years ) 3 groups : all groups got 700 g salmon / week with low , moderate or high content of n - 3 pufan - 3 content in salmon : low n - 3 : 0.5 g epa + dhamoderate n - 3 : 1.5 g epa + dhahigh n - 3 : 2.9 g epa + dha6 weeks il - 6 , ( high n - 3 ) svcam - 1 ( high n - 3 ) all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise statedmi myocardial infarction , pad peripheral arterial disease , chd coronary heart disease a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in healthy individuals all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated * larger change in crp and il - 6 with fish oil with low content of epa and dha compared to fish oil with high content ala - linolenic acid ( c18 ; 3 , n - 3 ) , gla - linolenic acid ( c18 ; 3 , n - 6 ) , aa arachidonic acid ( c20 : 4 , n - 6 ) a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with high cvd risk all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated positive correlation between change in serum n - 3 pufa and svcam - 1 tg triglycerides , oa oleic acid ( c18 : 1 , n - 9 ) , la linoleic acid ( c18 : 2 , n - 6 ) a review of marine n - 3 fatty acid intervention studies and circulating inflammatory markers in individuals with cvd and cvd related diseases all the intervention studies with n - 3 supplements were placebo - controlled ; double - blinded with parallel - design if not otherwise stated mi myocardial infarction , pad peripheral arterial disease , chd coronary heart diseasedetails of the 35 studies selected in this review are given in tables 2 , 3 and 4 . twenty - nine of these are based on marine n - 3 fatty acids given as supplements , whereas six studies are dietary interventions with fish . the systematic review of the literature reveals that seven out of thirteen trials reported an effect on circulating inflammatory markers in healthy individuals ( table 2 ) . six of the studies did not observe any changes in inflammatory markers ( table 2 ) . c - reactive protein ( crp ) was analyzed in seven of the trials , and in five of these no effect in crp levels was observed , while a reduction in plasma concentration of crp after intake of marine n - 3 fatty acids was reported in two of the trials . two of these reported a reduction in il - 6 levels , whereas three did not demonstrate any effect on il - 6 levels . table 2 also reveals that marine n - 3 fatty acids reduced or had no effects on the concentration of sicam - 1 in five of the trials . the concentration of svcam - 1 was reduced , increased or unchanged after intake of marine n - 3 fatty acids among healthy individuals in these trials , whereas the level of se - sel increased or remained unchanged . the effects of marine n - 3 fatty acids on circulating inflammatory markers among individuals with a high risk of developing cvd are shown in table 3 . four of fifteen trials reported a reduction in the concentration of inflammatory markers after intake of marine n - 3 fatty acids , while nine studies did not observe any effects on inflammation . one of nine studies where crp was analyzed demonstrated a reduction in the concentration of crp after intake of marine n - 3 fatty acids . three trials demonstrated a reduction in plasma cytokines or soluble adhesion molecules . table 4 reviews the effects of marine n - 3 fatty acids on circulating inflammatory markers among individuals with cvd or cvd related diseases . five of seven studies showed no change in the concentration of inflammatory markers after intake of marine n - 3 fatty acids , whereas one study reported a reduction in the levels of il - 6 and svcam - 1 and one trial showed an increase in the level of se - sel and svcam -1 . intervention trials with marine n - 3 fatty acids administered either from fish or fish oil supplements demonstrate different results on circulating inflammatory markers in healthy individuals , individuals with high risk of developing cvd or individuals with cvd related diseases . based on these findings , a firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can not be drawn . the majority of the trials do not show any effect , however a decreased level of circulating crp and il - 6 are observed in some studies , whereas the circulating level of adhesion molecules inconsistently are elevated , decreased or not changed . first of all , a local inflammatory improvement in the arterial wall will not necessary be reflected in the circulation and may explain the lack of effect observed after intake of marine n - 3 fatty acids . several of the reported trials have a relative small number of study subjects and probably low statistical power . variation in the results can also be explained by differences in the administration of the marine n - 3 fatty acids ( supplement vs. diet ) as well as the doses used . furthermore , there is uncertainty regarding the background diet and to what extend the intervention changed the balance between n - 3 and n - 6 fatty acids or altered the intake of saturated fat in the diet . comparing the results on the effects of marine n - 3 fatty acids given as supplements on serum crp in healthy individuals , the baseline level of crp seems crucial . reported a significant reduction in the level of crp in postmenopausal women who had mean baseline levels of crp above 6 mg / l . , vega - lopez et al . and madsen et al . who did not observe any changes in the crp serum levels . the baseline level of serum crp in the subjects in these studies was approximately 1vega - lopez et al . failed to see any effect on the serum crp level using a daily dose of 0.6 g epa and 0.9 dha for 12 weeks , which is in line with the results from madsen et al . who used two different daily doses ( 3.0 g epa + 2.9 g dha or 0.9 g epa + 0.8 g dha ) . in contrast , ciubotaru et al . observed in postmenopausal women that the effect on crp is dependent on the dose of marine n - 3 fatty acids . a larger decrease in crpwas observed in the women who received the highest dose of marine n - 3 fatty acids ( 2.56 g / d ) compared to those receiving a daily dose of 1.3 g marine n - 3 fatty acids . it is interesting to note that observed decrease in circulating crp level was associated with decrease in circulating il - 6 level after intake of marine n - 3 fatty acids , both via supplement or diet . in this reviewthere are relatively few studies where marine n - 3 fatty acids are administered as fish . when comparing these interventions with the interventions with fish oil , there are indications for a more prominent effect on circulating inflammatory markers with fish containing diet . reductions or no change in one of the following markers ( crp , il - 6 , sicam - 1 , svcam - 1 , il - 18 , se - sel ) are observed in all the six dietary studies included in this review . a possible explanation could be that subjects participating in dietary intervention trials may change their overall dietary pattern resulting in more awareness of healthy lifestyle , which again may influence the circulating inflammatory markers . fish may also contain other bioactive compounds that may influence outcome . to further understand if marine n - 3 fatty acids have an effect on inflammation in relation to atherosclerosis and the underlying molecular mechanisms responsible for their inflammatory response , new strategies should be considered . when inflammatory markers are measured in the circulation , the local effect in monocytes will not necessary be detected since the monocytes only contribute to about 5 % of the total cells in plasma . the peripheral blood mononuclear cells ( pbmcs ) include monocytes and lymphocytes , which are cells central in inflammation and hence in the atherosclerosis process . the pbmcs are exposed to many of the same environmental factors as the arterial wall . alterations in gene expression levels in these cells can thus be demonstrated early in the process before signs of inflammation can be seen in vivo , and thus constitute an important source of biomarkers for progression of atherosclerotic disease . pbmcs are easily available , which makes them suitable for studying gene expression of mediators involved in the early development of atherosclerosis . a previous study recently demonstrated that 166 genes among 182 candidate cardiovascular genes were expressed in pbmcs . the ability of marine n - 3 fatty acids to alter gene expression has been clearly demonstrated in vitro and may account for its potential beneficial health effects . fatty acids regulate expression of genes involved in lipid metabolism and inflammation by acting as ligands for the peroxisomal proliferator - activated receptors ( ppars ) . fatty acids are also known to reduce the activation of the transcription factor nuclear factor kappa b ( nf - b ) probably by interference with the ppars or the toll - like receptors . the g protein - coupled receptor 120 ( gpr120 ) has recently been characterized as an n - 3 fatty acid receptor / sensor involved in the anti - inflammatory effects of n - 3 fatty acids . dietary supplements containing fish oil have also been shown to affect expression of pi3k , akt , nf - b and inflammatory cytokines in mononuclear cells . to obtain a more comprehensive overview of the processes that are modulated by epa and dha , newomics technologies can be used to detect large scale changes in gene expression profiles ( transcriptomics ) . whole genome transcriptomic analysis in pbmcswill be valuable in further understanding the inflammatory role of n - 3 fatty acids in humans . recently , a human intervention study among elderly who daily consumed fish oil ( containing 1.8 g epa and dha ) for 26 weeks demonstrated changes in the gene expression profiles in pbmcs . gene transcripts involved in inflammation and other processes of atherosclerosis were down - regulated among those who had consumed fish oil . the combined use of other omics technologies , such as proteomics and metabolomics ( lipidomics ) , in intervention studieswill further extend the understanding of the effects of marine n - 3 fatty acids on inflammation . using proteomics technology , a recent study demonstrated that daily intake of 3.5 g fish oil down - regulated a number of proteins involved in the acute phase response and lipid / lipoprotein metabolism . furthermore , lankinen et al . previously used a lipidomics approach in an intervention study where 33 individuals randomized to eat fatty fish ( 150 g four times per week ) , lean fish ( 150 g four times per week ) or no fish , in 8 weeks . they observed a reduction in the level of lipid metabolites related to inflammation and insulin - signaling among those eating fatty fish . no firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can be drawn in this review . a systematic use of transcriptome , proteome , and metabolome technologies for the construction of network based models of biological processes has emerged as an exciting research approach in molecular biology and functional genomics . in futuremarine n - 3 supplementation and dietary intervention trials , systems biology combined with traditional circulating markers may help us to gain more insight into the underlying molecular mechanisms of marine n - 3 fatty acids responsible for the inflammatory response in the progression of atherosclerotic disease . intervention trials with marine n - 3 fatty acids administered either from fish or fish oil supplements demonstrate different results on circulating inflammatory markers in healthy individuals , individuals with high risk of developing cvd or individuals with cvd related diseases . based on these findings , a firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can not be drawn . the majority of the trials do not show any effect , however a decreased level of circulating crp and il - 6 are observed in some studies , whereas the circulating level of adhesion molecules inconsistently are elevated , decreased or not changed . first of all , a local inflammatory improvement in the arterial wall will not necessary be reflected in the circulation and may explain the lack of effect observed after intake of marine n - 3 fatty acids . several of the reported trials have a relative small number of study subjects and probably low statistical power . variation in the results can also be explained by differences in the administration of the marine n - 3 fatty acids ( supplement vs. diet ) as well as the doses used . furthermore , there is uncertainty regarding the background diet and to what extend the intervention changed the balance between n - 3 and n - 6 fatty acids or altered the intake of saturated fat in the diet . comparing the results on the effects of marine n - 3 fatty acids given as supplements on serum crp in healthy individuals , the baseline level of crp seems crucial . reported a significant reduction in the level of crp in postmenopausal women who had mean baseline levels of crp above 6 mg / l . , vega - lopez et al . and madsen et al . who did not observe any changes in the crp serum levels . the baseline level of serum crp in the subjects in these studies was approximately 1vega - lopez et al . failed to see any effect on the serum crp level using a daily dose of 0.6 g epa and 0.9 dha for 12 weeks , which is in line with the results from madsen et al . who used two different daily doses ( 3.0 g epa + 2.9 g dha or 0.9 g epa + 0.8 g dha ) . in contrast , ciubotaru et al . observed in postmenopausal women that the effect on crp is dependent on the dose of marine n - 3 fatty acids . a larger decrease in crpwas observed in the women who received the highest dose of marine n - 3 fatty acids ( 2.56 g / d ) compared to those receiving a daily dose of 1.3 g marine n - 3 fatty acids . it is interesting to note that observed decrease in circulating crp level was associated with decrease in circulating il - 6 level after intake of marine n - 3 fatty acids , both via supplement or diet . in this reviewthere are relatively few studies where marine n - 3 fatty acids are administered as fish . when comparing these interventions with the interventions with fish oil , there are indications for a more prominent effect on circulating inflammatory markers with fish containing diet . reductions or no change in one of the following markers ( crp , il - 6 , sicam - 1 , svcam - 1 , il - 18 , se - sel ) are observed in all the six dietary studies included in this review . a possible explanation could be that subjects participating in dietary intervention trials may change their overall dietary pattern resulting in more awareness of healthy lifestyle , which again may influence the circulating inflammatory markers . to further understand if marine n - 3 fatty acids have an effect on inflammation in relation to atherosclerosis and the underlying molecular mechanisms responsible for their inflammatory response , new strategies should be considered . when inflammatory markers are measured in the circulation , the local effect in monocytes will not necessary be detected since the monocytes only contribute to about 5 % of the total cells in plasma . the peripheral blood mononuclear cells ( pbmcs ) include monocytes and lymphocytes , which are cells central in inflammation and hence in the atherosclerosis process . the pbmcs are exposed to many of the same environmental factors as the arterial wall . alterations in gene expression levels in these cells can thus be demonstrated early in the process before signs of inflammation can be seen in vivo , and thus constitute an important source of biomarkers for progression of atherosclerotic disease . pbmcs are easily available , which makes them suitable for studying gene expression of mediators involved in the early development of atherosclerosis . a previous study recently demonstrated that 166 genes among 182 candidate cardiovascular genes were expressed in pbmcs . the ability of marine n - 3 fatty acids to alter gene expression has been clearly demonstrated in vitro and may account for its potential beneficial health effects . fatty acids regulate expression of genes involved in lipid metabolism and inflammation by acting as ligands for the peroxisomal proliferator - activated receptors ( ppars ) . fatty acids are also known to reduce the activation of the transcription factor nuclear factor kappa b ( nf - b ) probably by interference with the ppars or the toll - like receptors . the g protein - coupled receptor 120 ( gpr120 ) has recently been characterized as an n - 3 fatty acid receptor / sensor involved in the anti - inflammatory effects of n - 3 fatty acids . dietary supplements containing fish oil have also been shown to affect expression of pi3k , akt , nf - b and inflammatory cytokines in mononuclear cells . to obtain a more comprehensive overview of the processes that are modulated by epa and dha , newomics technologies can be used to detect large scale changes in gene expression profiles ( transcriptomics ) . whole genome transcriptomic analysis in pbmcswill be valuable in further understanding the inflammatory role of n - 3 fatty acids in humans . recently , a human intervention study among elderly who daily consumed fish oil ( containing 1.8 g epa and dha ) for 26 weeks demonstrated changes in the gene expression profiles in pbmcs . gene transcripts involved in inflammation and other processes of atherosclerosis were down - regulated among those who had consumed fish oil . the combined use of other omics technologies , such as proteomics and metabolomics ( lipidomics ) , in intervention studieswill further extend the understanding of the effects of marine n - 3 fatty acids on inflammation . using proteomics technology , a recent study demonstrated that daily intake of 3.5 g fish oil down - regulated a number of proteins involved in the acute phase response and lipid / lipoprotein metabolism . previously used a lipidomics approach in an intervention study where 33 individuals randomized to eat fatty fish ( 150 g four times per week ) , lean fish ( 150 g four times per week ) or no fish , in 8 weeks . they observed a reduction in the level of lipid metabolites related to inflammation and insulin - signaling among those eating fatty fish . no firm conclusion about the effect of marine n - 3 fatty acids on circulating inflammatory markers can be drawn in this review . a systematic use of transcriptome , proteome , and metabolome technologies for the construction of network based models of biological processes has emerged as an exciting research approach in molecular biology and functional genomics . in future marine n - 3 supplementation and dietary intervention trials , systems biology combined with traditional circulating markers may help us to gain more insight into the underlying molecular mechanisms of marine n - 3 fatty acids responsible for the inflammatory response in the progression of atherosclerotic disease .
output:
