SRL for [phosphorylated]: To first address the former possibility , we [phosphorylated] PTEN in vitro with CK2 and analyzed the phosphorylation by autoradiography , tryptic peptide mapping , phosphoamino acid analysis , and tandem mass spectrometry .
ARG-M-PRP: To first address the former possibility | ARG-0: we | ARG-1: PTEN | ARG-M-LOC: in vitro | ARG-2: with CK2