a culture equipment for bio - organisms such as animal cells and plant cells , having substratum and a circulation - inducing chamber , accommodating the material for substratum made of fibrous , or porous , or layered substance , such as of ceramics , plastics , resin or skin having a number of spaces in the substratum for adhesion or immobilization of microorganisms and medium permeation in the lid - containing vessel , and circulating the culture medium by a rotater device in the circulation - generating room is described in the present invention . the gas exchange of the culture medium is performed in the upper part of the vessel in the course of circulation . growth and maintenance of micro - organism cells in mass and at a high density can be attained .