the present invention discloses a new and improved kind of chromatographic cake and its manufacturing methods and applications . the objective of this invention is to provide an improved chromatographic apparatus and methods for manufacturing such an apparatus , whereby the separation and simultaneous renaturation and purification of biopolymers can be efficiently performed with excellent results . the chromatographic cakes of this invention generally comprise a chromatographic packing cake with a mobile phase inlet and a mobile phase outlet , together with a chromatographic packing packed into an inner cavity region of the chromatographic packing cake . the ratio of the thickness to the diameter of the inner cavity region of the chromatographic packing cake is less than or equal to 1 . a method of manufacturing such chromatographic cakes generally comprises the steps of : 1 ) manufacturing the chromatographic packing cake , wherein the chromatographic packing cake includes combined upper and lower clamp plates having , respectively , a mobile phase inlet or outlet , and a cake body with at least one lateral hole or aperture ; wherein the ratio of the thickness to the diametral dimension of the inner cavity of the chromatographic packing cake is smaller than or equal to 1 ; and , 2 ) adding chromatographic packing in whole or at least in part using the lateral hole of the chromatographic packing cake to fill the inner cavity of the chromatographic - packing cake with a suitable packing material .