the invention relates to the use of a pharmaceutical composition for the local treatment or prevention of a tissue infection at an infection site , the pharmaceutical composition comprising at least two different antibiotics of group a or pharmaceutically acceptable derivatives thereof , or an antibiotic of group a and at least one antibiotic of group b or pharmaceutically acceptable derivatives thereof . group a comprises primarily intracellular active antibiotics working as inhibitor of bacterial rna polymerase ; as inhibitor of gyrase ; or as inhibitor of bacterial protein synthesis . group b comprises primarily extracellular active antibiotics working as inhibitor of bacterial cell wall synthesis ; or inhibitor of bacterial protein synthesis ; or by direct destabilization or rupture of the bacterial cell wall . the invention further relates to a pharmaceutical composition for treatment of extracellular and / or intracellular microbial infected cells and / or for the prevention of microbial cell infections comprising at least one antibiotic acting as an inhibitor of bacterial rna polymerase and / or at least one antibiotic affecting the bacterial cell wall or its synthesis , and a substrate carrying a pharmaceutical composition . the invention also relates to the use of a combination of at least one antibiotic acting as an inhibitor of bacterial rna polymerase and at least one antibiotic affecting the bacterial cell wall or its synthesis as anti - adhesive against microorganisms on surfaces .