a method for implanting cells onto a prosthesis includes the steps of : providing a prosthesis including a porous tube , where at least 25 % of the pores on the inner surface of the tube have diameters of more than about 40 μm , at least 25 % of the pores on the outer surface of the tube have diameters of less than about 30 μm , and the tube includes a substantially continuous layer of a biocompatible material ; contacting the prosthesis with a suspension of cells ; and providing a pressure differential between the inner surface and the outer surface , whereby the cells are retained in the pores of the inner surface . a method for obtaining an endothelial cell culture from a blood sample involved : obtaining a sample for mononuclear cells from a blood sample ; and culturing the sample of mononuclear cells , without further cell separation , on a cell adhesive polymer - coated solid support in the presence of endothelial growth factors .