with respect to molecular biology , medicine , and biotechnology , provided is a method related to the performance of polymerase chain reaction . the method includes using the device for identification of nucleic acids containing a microchip with at least one reaction zone on its surface . the microchip contains a heat - conducting substrate made of aluminum while each reaction zone is separated from the heat - conducting substrate by a layer of the passivating material covalently bound to the surface of the heat - conducting substrate while over the layer of the passivating material one or several dried components of the polymerase chain reaction is placed . in this method the ratio of the aggregate thermal mass of the microchip to thermal conductance of the microchip substrate does not exceeding 0 . 04 s . the technical result is reduced duration of the analysis , higher reliability , accuracy , efficiency and cost - effectiveness of the analysis .