disclosed is a bio - electrospinning technique for preparing a cell - containing , oriented , continuous tubular scaffold , made of biodegradable polymer , designed for use as a nerve guide conduit in nerve regeneration . with a coaxial spinneret , the pc - 12 cell medium solution was co - electrospun into a core of tubular fibers , with pla on the outer shell . the resulted fibers &# 39 ; morphology was characterized via sem and optical microscopy , and following structural characteristics were found : 1 . the larger , hollow fibers had diameters in tenth of microns and wall thicknesses around few microns , 2 . an orientation in a preferred direction with the aid of a high - rotating collection device . the fluorescent pc12 cells embedded within the scaffold were cultured and nerve growth factor was added . we observed cells could not only survive the process , but also sustain their viability by undergoing differentiation process , extending neurite along the micro tubular scaffold in the desired direction . all these results demonstrate its potential application for advanced ngc .