a thermosensitive hepatitis b vaccine is provided . the thermosensitive hepatitis b vaccine includes an aqueous phase solution comprising a biodegradable thermosensitive hydrogel copolymer ; a surface antigen of hepatitis b virus ; and a bioactive substance . the thermosensitive hepatitis b vaccine of the disclosure is particularly suitable for being applied in the patients , which are low responsive or non - responsive to conventional hepatitis b vaccine , for enhancing the induction of cell - mediated immune responses and overcoming the hbsag non - responsiveness .