a type of liquid chromatographic stationary phase and preparation method thereof , the bonding terminal of the chromatographic stationary phase is zwitterionic functional group . the preparation method includes the following steps , alkenyl or alkynyl silane is bonded onto the surface of silica based on the horizontal polymerization approach to obtain alkenyl - or alkynyl - modified silica . then the thiol click reaction with zwitterionic compound containing thiol group is performed to obtain the zwitterionic hydrophilic interaction chromatographic stationary phase . the present stationary phase possesses both zwitterionic characteristics and excellent hydrophilicity . it can be widely applied in the separation of variety of samples .