the present invention relates to biofunctional polymeric substrates product comprising immobilized antibodies that selective bind to antigens namely autologous growth factors and an inflammatory molecule , uses and method of production . the bio - functional polymeric substrate can be used in a quantification method of antigens namely soluble growth factors , inflammatory molecules and hormones or the biofunctional polymeric substrate can be used to isolate extracellular vesicules . the biofunctional polymeric substrate can be also used in tissue engineering and regenerative medicine approaches , namely in bone and cartilage lesions , esophageal lesions , periodontal ligament reconstruction and skin lesions regeneration or treatment .