the present invention relates generally to an apparatus and kit in the microtiter plate format for improved multiplexed microarraying . the apparatus overcomes current limitations with conventional polymeric and glass bottom microtiter plates . the invention also relates to an apparatus for conducting multiple biomolecular assays comprising a low self - fluorescent coated substrate having an upper and lower surface . the substrate upper surface has a pattern comprising a plurality of wells . a flexible superstructure having a plurality of through holes in a pattern that is similar to and aligns with the wells on the substrate is removably adherable to the upper surface of the substrate . preferably , the superstructure is adhesively attachable to the substrate . a tray supports the substrate and the superstructure and acts as an alignment jig . in a preferred embodiment the substrate is low self - fluorescent glass and coated on the upper surface with a functional coating to which other biomolecules can bond .