this invention relates to compositions and methods for cleaning , disinfection , and sterilization of hard , soft , and porous surfaces , equipment , human skin , tissues , food and vegetable surfaces , and other media which are contaminated with microorganisms such as bacteria , viruses , yeast , and molds . particularly relevant is the microbial environment whereby bacterial communities can generate three - dimensional polymicrobial extracellular polymeric substances , or eps , biofilm communities supported by an aggregation of microorganisms growing on a substrate layer , substantially degrading the antimicrobial performance of typical cleaners , disinfectants , and sterilizers . there is great need for a composition that will reliably eradicate microorganisms across a broad spectrum , particularly polymicrobial eps communities shrouded by self - generated biofilm layer . such a composition is disclosed herein . the invention discloses new compositions , and methods for formulation of compositions , of weak acids , surfactants , and glycol monoesters , which achieve a level of eradication such that when tested following application , no surviving microorganisms remain . all compositions and methods disclosed herein have the further benefit of their ingredients being classified by the us food and drug administration as gras , or generally regarded as safe for use on food and food contact surfaces ; and the embodiment of the claimed invention herein contains 95 % usda certified biobased content , and is accepted to the usda biopreferred ® program .