the developed oligonucleotide microchip for simultaneous , rapid identification of multiple crucial forest phellinus pathogens was based on the dig or biotin - labeled specific probes derived form ribosomal dna genes , by using reverse - dot hybridization . the chip can precisely and accurately identify and diagnose seventeen phellinus species , including notorious hardwood and conifer tree killer , p . noxius and p . weirii , with a sensitivity of 1 pg dna / μl on nylon membrane , and 100 fg dna / μl on plastic chip , respectively . verification and identification of forest phellinus pathogens infested authentic samples or voucher specimens can be accomplished within 7 hr .