the present invention relates to a method of immobilizing proteins on a polymeric matrix by means of plasma activation and an apparatus and process for the use of such material . the protein mixture is applied to the surface of the polymeric matrix with or without the addition of a crosslinking agent . if is then placed into a plasma generator , wherein the functional groups on both the protein and the matrix molecules are activated to form free radicals . upon returning from their high energy state , the free radicals form covalent bonds between the proteins and between the protein and the polymeric matrix . using this method , the proteins are nonspecifically immobilized on the surface of the polymeric matrix . the method can be utilized to immobilize proteins on the surface of polymeric membranes , polymeric beads , polymeric tubes and polymeric plates . the immobilized protein has high biological activity and stability .