disclosed are improved methods for preparing high resolution replicas for electron microscopic analysis . replicas are prepared having a resolution capability less than ten angstroms , with structures of even less than five angstroms being readily discernible . particular aspects of the invention concern the use of a platinum / carbon evaporant to prepare extremely thin replica surfaces which allow the visualization of structures heretofore unresolvable . the replication is performed at high vacuum , on the order of 10 - 5 torr or better , with continuous rotation of the sample to allow for an even distribution of the evaporant . the resultant replica surfaces are on the order of no more than 5 to 10 angstroms thick . the replica technology disclosed herein is applicable to both biological as well as non - biological samples , including tissues , biochemicals , metals and polymers and even computer chips and superconductor surfaces .