the subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls . the subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin - degrading enzymes or tubulin de - polymerizing compounds including colchicine . the subject invention also relates to novel uses of compounds for such purposes . another aspect of the subject invention relates to transformation of the subject , isolated cells . such processes simplify and integrate single - cell - based transformation and selection processes into transgenic and transplastomic event - generation work processes . the subject invention also removes technical constraints and produces marker - free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health , biopharma , and trait and crop protection platforms .