the disclosure relates to a bio - artificial periosteum based on micropatterning of biomimetic mineralized calcium - phosphorus nanoparticles and a method for manufacturing the same . the method includes : first , a micropatterned biomimetic mineralized calcium - phosphorus nanoparticle layer is manufactured on a surface of an inert substrate ; then , an organic polymer is cross - linked and solidified on the micropatterned biomimetic mineralized calcium - phosphorus nanoparticle layer ; at last , the inert substrate is removed , so that the bio - artificial periosteum based on micropatterning of biomimetic mineralized calcium - phosphorus nanoparticles is obtained . the bio - artificial periosteum not only simulates the composition of natural bone in material components , but also realizes high degree of biomimesis in micro - nano size in structure . moreover , the distribution of bone marrow mesenchymal stem cells can be regulated by the bio - artificial periosteum , so that the cells can be effectively defined on a surface of calcium - phosphorus particle micropattern and a high degree of ordered alignment thereof can be realized .