a unitary counting device for cells and other microscopic particles is described in this invention . the invention also relates to the method of producing the counting device and to the method of using the device . the counting device is constructed with a top part , a connection layer , a base part , and a grid of microscopic lines that are built inside a counting chamber to define counting areas . furthermore , the counting chamber has a sample introduction port and an air escape port . a connection layer bonds the top part and the base along perimeter of the chamber and maintains gap uniformity . the connection layer consists of a polymer film sandwiched by two layers of pressure sensitive adhesive . grid lines are fabricated using polymerizable solutions , for narrow and thin lines , which enable counting small particles under high magnifications .