polyelectrolyte based bioactive super - absorbent material ionotropically crosslinked / neutralized involving polyvalent carboxylic acids , citrate , kojic acid , alpha arbutin along with fish scale collagen cross linked with other polyelectrolyte biopolymers preferably selected from chitosan , alginate or their combinations is used in this invention . the advancement is also directed to process of extraction of collagen of high purity from fresh water fish scale by salt and alkaline washing , crushing followed by continuous dialysis thereby minimizing the chance of collagen degradation . also disclosed are different forms of collagen - chitosan composite biomaterial using citrate as the neutralizing buffer in combination with antimicrobial agent , antioxidant , skin plumper and melanin reducer wherein the different forms of collagen chitosan particularly sheet , flakes , powder , gel , particles , fiber , film , spray etc . reveal efficient wound healing properties . the advancement is thus directed to find wide application in various dermal wound healing , tissue engineering , 3d cell culture , cell expansion and cell delivery vehicle , mimicking the in vivo situation in dynamic condition , cosmetics and different other health care applications .