provided is a fusion protein comprising a tumor necrosis factor related apoptosis inducing ligand , integrin ligands of αvβ3 and αvβ5 and a linking peptide . also provided are the expression method and simple separation and purification process for the production of the fusion protein which is soluble and has high content of the polymer , and use of the fusion protein for the manufacturing of a medicament for the treatment of tumor . the fusion protein has good tumor tissue targeting property , significantly enhanced anti - tumor effect , which can also reduce the dose of the needed protein for the target treatment effect , improve the bioavailability , reduce the treatment cost , decrease and overcome the potential toxic and side effects of the tumor necrosis factor - related apoptosis inducing ligand .