the present invention provides a chromatography medium comprising one or more electrospun polymer nanofibers which form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same in chromatography , such as the isolation of recombinant proteins , monoclonal antibodies , viral vaccines and plasmid dna . the invention further provides for the use of the chromatographic medium in a simulated moving bed system .