the present subject matter relates to light - activatable polymeric nanoparticles for the transportation and release of an active substance , methods for obtain said particles and their uses . a light - activatable nanoparticle for the transportation and release of an active substance , comprising a polycation preferably a polimer polycation , a polyanion and a light - sensitive photochrome attached to the polycation or the polyanion , wherein said photochrome is hydrophobic and suitable to photo - cleave when activated by an irradiation source , generating a negative charge and releasing the active substance . light - activatable . the disclosure subject matter shows that nps are a highly efficient drug delivery system to primary leukemic cells based on opto - nanomedicine system . therefore , the present disclosure is useful for remote control in the release of biomolecules with spatio - temporal resolution with applications in the areas of general therapeutic and regenerative medicine applications .