this invention is related to preparation of photosensitive ruthenium based aminoacid monomers and oligomers , aminoacid monomer - protein cross - linking using photo sensitat ion and conjugation on micro and nano - structures by ruthenium - chelate based monomers . its vast range biotechnolgy applications of multifunctional , biocompatible , stabile and specific micro and nanobio - conjugates , which will stand - alone or simultaneously enable both purification and determination , both targeting and imaging and theranostics and catalysis and determination . the construction and method of preparation is applicable to silica materials , superparamagnetic particles , qds , cnts , ag / au nanoparticles and au surfaces and polymeric materials . the photosensitive aminoacid monomer linkers can react via chemically and biocompatible to a lot of different micro and nano - surface and then to the protein when they act as a single - step cross - linking reaction using irradiation . the photosensitive conjugation based on click biochemistry can be carried out at mild conditions , independent of ph and temperature , without affecting conformation and function of protein .