the present invention is related to an immuno - pcr method for detecting nasopharyngeal carcinoma and kit thereof , especially related to an immuno - pcr method for detecting markers of early stage npc and kit thereof . the present invention includes providing a substrate whereon protein markers immobilized ; applying a patient &# 39 ; s specimen to the substrate ; adding a solution which has biotinylated anti - human iga secondary antibody and incubating the solution ; adding a solution with a linker and biotinylated target dna ; proceeding a polymerase chain reaction ; and finally , detecting the target dna fragments via electrophoresis .