the present invention provides liver parenchymal cells having a clonal growth ability , which possesses at least one of the cell biological properties such as : presence of peroxysome ; being positive to hepatocyte - markers ; being partially positive to neoplastic hepatocyte - markers or immature hepatocyte - markers ; being positive to antibodies against the surface antigens of ovall cells ; and being partially positive to bile duct cell - markers . the present invention also provides a method for obtaining such cells and a method for subculturing such cells . with the liver parenchymal cells above , it will be possible to research in detail the process of development and differentiation , the mechanisms of growth and functional expression of hepatic cells , and to open up a new way to clalification of mechanisms or hepatoma and various other diseases and to development of therapeutic method against these disseases .