the allosteric effectors , sulfate and phosphate , can be used with mannanohydrolase enzymes to better control the break of gelled fracturing fluids in wellbore operations . the fracturing fluid contains a hydratable polymer of guar , a guar derivative , a cellulose derivative , a water soluble biopolymer or a combination thereof and a crosslinking agent . the mannanohydrolase enzyme catalyzes the hydrolysis of β - mannosidic linkages to break the polymeric backbone .