the present invention relates to biochemistry , medicine , and molecular biology , in particular to analytical biochemistry and immunochemical assay , and is concerned with a biological microchip for multiple parallel detection and quantitative determination of different compounds . the biochip comprises an array of three - dimensional hydrogel elements which have a predetermined volume , formed on a support by the method of photo - or chemically induced polymerization , and containing biological molecules of the same or different nature . the invention provides a method for detecting compounds on the biological microchip , comprising identification thereof by mass spectrometry techniques directly on the hydrogel element of the microchip and a method for detecting immunoassay results , consisting in carrying an immuno - pcr and registering the results thereof on the same microchip . the invention also relates to a method of multiple parallel immunoassay of compounds on a biological microchip . biological microchips for immunoassay and a method of carrying out multiple parallel analysis of compounds can find application in analyzing a wide range of high - molecular and low - molecular compounds , in medicine , pharmacology , food industry , environmental protection , in research work , particularly in proteomics .