an enzyme electrode sensor and a fabricating method thereof , and more particularly , an enzyme electrode sensor which is a biosensor using electrochemical measurement and a manufacturing method thereof . the sensor includes an electrode , a first nonconducting polymer layer formed by electropolymerization outside the electrode wherein enzyme is immobilized in the nonconducting polymer layer , a second nonconducting polymer layer in which enzyme is not immobilized , the second nonconducting layer formed by electropolymerization outside the first nonconducting polymer layer , and an outer layer formed outside the second nonconducting layer . the sensor selectivity is improved as the interference of organic materials is inhibited , and the interference of acetaminophen causing the major problem with a glucose sensor is controlled effectively by the sensor .