a method for preparing a porous - three - dimensional scaffold good for tissue engineering is described . sericin forms a three - dimensional scaffold with pva after freeze - drying having glycerin as a plasticizer and genipin as natural crosslinking agent to help making a strong and stable matrix . adding glycerin into scaffold gives good uniformity and porosity . smaller pore sizes and better uniformity are obtained as the concentration of genipin in the scaffold increases . glycerin retains a high moisture content to allow the presence of water molecule in the matrix structure . adding genipin results in a higher degree of crosslinking within the scaffold . crosslinking using genipin is most beneficial in preparing scaffold possesses the best biological and physical properties for wound healing . the present invention describes method for preparing crosslinked matrix whose composition can be appropriately tuned to obtain matrix with desirable characteristics for biological applications .