it is intended to provide a three - dimensional scaffolds appropriate for culturing animal cells such as chondrocytes and fibroblasts . these three - dimensional scaffolds which comprise a chitosan / acidic biopolymers hybrid fibers composed of chitosan or its salts in the inside of the fiber and complexes of chitosan with biodegradable acidic biopolymers coating the fiber surface , are made of fibers capable of retaining their shapes after allowing to stand dulbecco &# 39 ; s modified eagle &# 39 ; s medium containing 10 % of fbs at room temperature for 2 weeks .