page_title: Buoyant density centrifugation
section_title: Implementation
hierarchical_section_title: Buoyant density centrifugation / Implementation
caption_attribution_description: English: A 6cm polystyrene tube containing caesium chloride (CsCl) solution and two morphological types of rotavirus. Following centrifugation at 100g a density gradient forms in the CsCl solution and the the virus particle separate according to their densities.
context_page_description: Buoyant density centrifugation uses the concept of buoyancy to separate molecules in solution by their differences in density.
context_section_description: Historically a caesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll. The sample is put on top of the solution, and then the tube is spun at a very high speed for an extended time, at times lasting days. The CsCl molecules become densely packed toward the bottom, so even layers of different densities form.  Since the original solution was approximately the same density, they go to a level where their density and the CsCl density are the same, to which they form a sharp, distinctive band.
caption: Caesium chloride (CsCl) solution and two morphological types of rotavirus. Following centrifugation at  100g a density gradient forms in the CsCl solution and the virus particle separate according to their densities.
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