page_title: Methylated DNA immunoprecipitation
section_title: Methods
hierarchical_section_title: Methylated DNA immunoprecipitation / Methods
context_page_description: Methylated DNA immunoprecipitation is a large-scale purification technique in molecular biology that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5-methylcytosine. This technique was first described by Weber M. et al. in 2005 and has helped pave the way for viable methylome-level assessment efforts, as the purified fraction of methylated DNA can be input to high-throughput DNA detection methods such as high-resolution DNA microarrays or next-generation sequencing. Nonetheless, understanding of the methylome remains rudimentary; its study is complicated by the fact that, like other epigenetic properties, patterns vary from cell-type to cell-type.
context_section_description: The following sections outline the method of MeDIP coupled with either high-resolution array hybridization or high-throughput sequencing.  Each DNA detection method will also briefly describe post-laboratory processing and analysis.  Different post-processing of the raw data is required depending on the technology used to identify the methylated sequences.  This is analogous to data generated using ChIP-chip and ChIP-seq.
caption: Workflow overview of the MeDIP procedure. MeDIP procedure is followed by array-hybridization (A) or high-throughput/next generation sequencing (B)