This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
Virus is cultured in: 
Select and explain. 
A. Sabouraud's agar, B. Rogosa medium, C. Nonembryonated egg, D. Embryonated egg
D: Embryonated egg
Answer: D: Embryonated egg 
Explanation: Embryonated eggs were used initially for the growth of viruses.
Embryonated chick egg was used first for cultivation of viruses by Goodpasture in 1931. 
Usually, 8–11 days old chick eggs are used for culture of viruses. The viruses are isolated in different sites of the egg, such as yolk sac, amniotic cavity, and allantoic cavity, and chorioallantoic membrane (CAM).