This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Biochemistry
Which of the following does not require 5'capping? 
Select and explain. 
A. tRNA of alanine, B. mRNA for histone, C. U6 snRNA, D. siRNA
A: tRNA of alanine
Answer: A: tRNA of alanine 
Explanation: Ans: A. tRNA of alanineSmall nuclear RNAs contain a unique 5'-caps. Sm-class snRNAs are found with 5'-trimethylguanosine caps, while Lsm-class snRNAs are found with S'-monomethyl phosphate caps.mRNAs do have a 7-methylguanylate cap, abbreted m7G.tRNAs and rRNAs don't require 5' capping. They have other modifications.