This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: None, Subject name: Microbiology
A patient presented to the hospital with severe hydrophobia. You suspect rabies, obtained corneal scrapings from the patient. What test should be done on this specimen for a diagnosis of rabies? 
Select and explain. 
A. Negri bodies, B. Antibodies to rabies virus, C. -PCR for rabies virus, D. Indirect immunofluorescence
C: -PCR for rabies virus
Answer: C: -PCR for rabies virus 
Explanation: Ans: C. -PCR for rabies virusRef: Ananthanarayan 10/e p536, 8/e p529: Harrison 19/e p1302).Detection of rabies virus:-PCR with genetic sequencing:Highly sensitive & specific.Accurately identify rabies genome (viral RNA) in CSF, fresh saliva samples, corneal scrapings or urine.Distinguish among rabies virus variants.Permits identification of probable infection source.Reverse transcription-polymerase chain reaction testing:Used to amplify rabies virus genome pas from fixed or unfixed brain tissue or saliva.Sequencing of amplified products allows identification of infecting virus strain.