This is a real-world medical entrance exam question, please give the true answer based on the question and selection. Topic name: AIIMS 2018, Subject name: Microbiology
In which of the following, viral load done by Real Time PCR is of no role in investigative procedures? 
Select and explain. 
A. Person with hepatitis B on Tenofovir therapy, B. HSV causing temporal encephalitis, C. BK virus in patient of allograft renal transplant, D. CMV PCR in blood of patient of liver transplant
B: HSV causing temporal encephalitis
Answer: B: HSV causing temporal encephalitis 
Explanation: In person with Hepatitis B on Tenofovir therapy along with patients infected with BK virus in patient of allograft renal transplant and in cytomegalovirus PCR in blood of patient of liver transplant viral load is necessary so viral load is done by real time PCR but it in hsv causing temporal aeritis there is a search no role of viral load so there is no such need of real time PCR This is replaced by HSV DNA in CSF by PCR