Method and instrument for the detection of neisseria gonorrheae without culture
United States Patent 91 Mennen [451 Apr. 8, 1975 METHOD AND INSTRUMENT FOR THE DETECTION OF NEISSERIA GONORRHEAE WITHOUT CULTURE [76] Inventor: Frederick C. Mennen, 506 Clay St..  
 LaPorte. 1nd. 46350 22 Filed: Oct. 29, 1971 211 App]. No.: 193,739  
 OTHER PU BLlCATlONS Hartman, Miniaturized Microbiological Methods, p. 5 (1968).  
 Primary Examiner-Alvin E. Tanenholtz Attorney, Agent, or Firm-Abraham A. Saffitz [57] ABSTRACT A non-culture method and instrument for a rapid presumptive test for gonorrhea in which a specimen of exudate from a suspected case is placed in direct contact with a pledget containing a compound which reacts with Neisseria gonorrheae to produce a color change. The pledget, before the test, is in a dry condition and is activated by a wetting agent such as saline, when placed in contact with the pledget. The chemical compound used and incorporated into the pledget is selected from a group consisting of phenylenediamines. The instrument used consists of a flexible tube containing a fluid-filled frangible ampul at one end and the chemically impregnated pledget disposed immediately above same. A separate sterile swab is supplied for taking the specimen. The swab containing the specimen is inserted into the flexible tube. The flexible tube is then squeezed at the ampul portion, releasing the wetting agent by breaking the frangible ampul. The wetting agent when thus released activates the reagents in the pledget. A downward pressure of the swab containing the specimen places it in direct contact with the activated pledget. 1f gonorrhea is present in the specimen on the swab, a distinctive coloration of the specimen takes place within 2 minutes.  
 3 Claims, 2 Drawing Figures METHOD AND INSTRUMENTFOR THE DETECTION OF NEISSERIA GONORRHEAE WITHOUT CULTURE It is common knowledge that this Country and most of the world is undergoing a venereal disease epidemic. In the United States alone the disease has reached pandemic proportions. It is estimated that only one-fifth of the cases are reported and only one-third reach the attention of physicians and Public Health authorities in order to receive treatment. The availability of a simple, rapid and inexpensive test would aid in recognition and control of this disease.  
  The usual clinical evidence of a gonorrheal infection in the male is a purulent discharge from the meatus and urethra of the penis. As routine procedure it is necessary to make adifferential diagnosis of the nature of the discharge before antibiotics can be prescribed. As a rule the first test is to determine if the urethritis is gonococcal or non-specific in nature. An object of this invention is therefore to provide&#39;a system which will operate directly from the patient and give the clinician or physician a differential diagnostic tool which is time saving, inexpensive and reliable.  
  As a screeningtest for gonorrhea in public V-D &#39;clinics, hospitals, physicians offices and the Armed Forces, the present device would serve as an inexpen# sive and accurate differential&#39;diagnostic aid to assist the physician or clinic in the choice of drugtreatment. The need for a simple and inexpensive diagnostic system that can function inthe field, independent of bacteriological and microscopic tests, is therefore well established. One of the difficulties in making a quick and reliable diagnosis of male infection is the initial confusing similarity with urethritis.  
  A urethral exudate in themale inay&#39;appear as a result of the following other causes: prostatitis, trauma, Escherichia coli, staphylococcus, tuberculosis, balanitis, ingested urethral irritants, cantharides, vegetables rich in oxalates, and others may precipitate urethral inflammation. Pellagra, diabetes and gout are responsible in: some cases. Trichomonas vaginalis can be found in fair frequency in abacterial urethritis. Entomoeba histolyt ica as a cause of urethral discharge is found exclusively in the presence of recto-urethral or vesical fistula. Bilfarizia is a metozoan that produces urethritis rather frequently in endemic areas. Such systemic diseases as typhoid, mumps, influenza and smallpox can, if septicemic, produce urethritis.  
  In a large percentage of individuals with acute or chronic urethral discharge, although suspected of having a gonorrheal infection, demonstration of the Neisseria is not possible. Many of these are treated as gono-&#39; coccal infections and it is only when they persist, subsequent to a variety of therapeutic measures, that their abacterial nature may be recognized. Subsequent thor-&#39; ough examination of the entire anterior and posterior urethra and upper urinary tract may reveal that the signs of inflammation are present but that structural deformity is not predispositional, and, further, that etiologic organisms are totally absent.  
  It has been estimated that 80 per cent of nonspecific urethritides are abacterial. Two; forms are prevalent: the acute type, resembling acute gonorrhea, and the subacute or Welsch type. Abacterial pyuric conditions of the prostate and upper urinary tract have been known to exist a long time, and occasionally the urethritis is associated with symptoms referable to the upper LII urinary tract. The acute abacterial urethritis usually has its inception one to five days after intercourse. A profuse purulent discharge occu&#39;rswith reddening of the meatus and accompanying dysuria. Posterior urethral involvement maybe indicated by frequency, urgency, and terminal hematuria&#39;; The clinical picture is so similar to that seen in gonorrhea that it is confusing when the Neisseria is not&#39;demonstrable.  
  The&#39; differential diagnosis of &#34;a urethral discharge rests upon exclusion of theurethritides appearing in gonorrhea as the first step, secondary&#39;to &#39;other&#39;bacte ria&#39;, foreign body, trauma, local neoplastic and specific inflammatory diseases, sensitivity reactions, urethral manifestations of systemic disease and infestation with protozoa, metazoa and fungi.  
  The principal objectof this invention is to provide a diagnostic system which will detect Neisseria gonorrhea in the male without culture or the classical gramstaining method, both of-which are time consuming and expensive to perform and require trained technicians and laboratory, equipment. I  
  The present invention involves the use of a reagent from the group of oxidase testing reagents known as phenylenediamines, including-the following: &#39;p-Amino Dimethylaniline Oxalate, N, N-Dimethyl-p- Phenylenediamine Dihydrochloride. N,&#39;N-Dimethyl-p- Phenylenediamine Oxalate, N., vN-Dimethyl-p- Phenylenediamine.Monohydrochloride and N, N, N  
 .N Tetra-Methyl-p-Phenylenediamine Dihydrochloride. A pledget or carrier of any suitable material such as dacron fiber, cotton fiber or other porous material is impregnated or saturated with one .of these reagents under certain conditions hereinafter disclosed and then dried. lt remains in the dry state until it is activated by a wetting agent.  
  The pledget is the principal component of the diagnostic system, as it contains the reactive chemical that is capable of identifying the gonococcus. In the dry state the pledget as prepared by this method will remain stable and is capable of long shelf life. The second part of the system is the wetting agent which. is separated from the pledget by virtue of being contained in a frangible ampul. Whenthe frangible ampul is crushed the wetting agent contained therein is released, activating the reagent in the pledget. The pledget after being highly sensitized is brought into contact with the specimen on the tip of the collecting swab, reacting with the gonococci present, causing the specimen located on the swab to take on a charactaristic color depending on the choice of reagentused. The pledget being nearly colorless in appearance does not causeia confusing reaction because &#39;it does not react colorimetrically. The color change takes place on the specimen collected on the swab. The reaction time to indicate a positive specimen usually falls within the rangeof from 30 to seconds.  
  The wetting agent contained in the ampul may consist of water, physiological saline, pH buffer solutions, or any other solutions or combinations of solutions which enhance or sensitize the oxidase reaction. The control of pH of the wetting agent is desirable to prevent autooxidation of the reagent-in the pledget. The preferred pH&#34; range of the wetting agent should be 6.5  
 to 7.2. I I  
  In order to illustrate more fully&#39;tlierija niier in which the test is made using the foregoing&#39;reagents&#39;, reference is made to the drawing, wherein:  
  FIG. 1 is an elevational view of one type of instrument suitable for use in practicing my method for detecting Neisseria gonorrheae, showing the instrument prior to use in a test in the form sold as a packaged system consisting of two basic parts; and  
  FIG. 2 is an elevational view of the instrument shown in FIG. 1 after it has been used in making a test.  
  Referring more specifically to the drawing, which represents one type of instrument capable of performing my test procedure, numeral indicates a tube of transparent, flexible plastic, into which is inserted a pledget 12 of cotton impregnated or saturated with the reagent and dried. The pledget is seated on a frangible glass ampul 14 disposed in the closed end of the tube and a cap 16 is placed over the open end of the tube, sterile swab 18 with a plastic handle 20 to be used in obtaining a specimen of exudate is sealed in a separate sterile envelope 22, and the tube and envelope are packaged together in an envelope or other suitable container (not shown). While the wall structure of the plastic tube is flexible, it has sufficient rigidity normally to maintain a generally cylindrical shape and to permit easy insertion of the swab after a specimen of exudate has been taken. The ampul contains a suitable wetting agent, as described herein, and is sufficiently frangible that it can readily be broken when the sides of the flexible tube are pressed inwardly between the thumb and forefinger. whereupon the ampul shatters and permits its fluid contents to wet the material impregnated in the pledget, which normally is pushed into the fluid and fragmented glass when the swab is inserted in the tube. When the swab containing the specimen contacts the pledget, reaction between the specimen on the swab and the reagent in the pledget commences immediately and, if gonococci are present, the specimen on the swab changes color, normally to purple, red-orange, or dark gray, depending upon the reagent used in the pledget, thus indicating a positive test. The reaction time to indicate a positive specimen usually falls within the range of from 30 to 120 seconds.  
  A preferred method selected from the above for the manufacture of the pledget and the preparation of a wetting agent is illustrated as follows:  
  A 1 percent solution of N, N, N N Tetra Methyl-p- Phenylenediamine Di-hydrochloride is prepared as follows: 1 gram of the reagent is added to 35 ml distilled water that has been brought to a boil and allowed to cool to room temperature. Solution is effected by rapid stirring. 64 ml of Ethyl Alcohol (Fisher A-407) is then added and stirred. Strips of cotton Webril, /1 inch wide 20 inches long, are then dipped one at a time into the solution, placed on nylon screen in a horizontal position and allowed to air-dry. The dry strips are then cut into pieces 9/16 inch long. These become the pledgets.  
  A physiological saline solution is prepared by adding 0.85 grams of Sodium Chloride to 99 ml distilled water and the pH adjusted to 7.0. This solution becomes the wetting agent. Frangible ampuls containing /2 ml of this solution are prepared, as this is the quantity necessary to adequately wet the /1 inch X 9/16 inch pledget.  
  The ampul, approximately 7 mm in diameter and 35 mm in length, is placed at the bottom of a flexible plastic tube approximately 8 mm inside diameter and 160 mm long. A pledget manufactured by the above technique is rolled up and pushed down the tube so that it is in contact with the ampul. A telescopic cap approximately 65 mm in length is positioned over the open end of the plastic tube, capping the system.  
  A sterile dacron swab, mounted on a plastic stick approximately mm long is packaged together with the reagent system and this combination becomes the diagnostic instrument. This swab is separately contained in its own sterile glassine envelope.  
  The above two units comprising a single package are adapted to be sold through normal pharmaceutical channels to physicians, clinics, and hospitals and the package is immediately available for a test and prompt diagnosis of the patients condition at the site of the specimen collection. When a diagnostic test for gonorrhea is to be made, the package is opened and the sterile swab is removed from its envelope, and a specimen of exudate collected to the tip of the swab. The telescopic cap is then removed from the plastic tube and the swab is inserted, tip first, into the plastic tube. The telescopic cap is then replaced. The flexible tube is then squeezed at the ampul portion, breaking same and releasing its contents. Simultaneously, the telescopic cap is pushed down, forcing the specimen containing swab into contact with the pledget. This action also forces the pledget down into the wetting agent, activating the system. The specimen on the swab turning purple within two minutes is a positive test for gonorrhea. The swab may be withdrawn from the pledget by about 1 inch to facilitate reading of the test results, i.e., the foregoing change in color of the specimen on the swab. The use of this test, therefore, gives the physician or clinician a rapid and accurate diagnostic tool in the first step of his differential diagnosis.  
  To illustrate further the sensitivity of this system and its ability to detect the gonococcus precisely at the point of specimen collection on the swab, the above procedure is repeated and a culture of Neisseria gonorrheae is used as being representative of the exudate in the foregoing example. In this example the swab is used to pick a few colonies from a petri dish culture. The identical procedure is used with the instrument. A distinct purple coloration of the bacteria on the swab will be observed within 1 minute. The nearly colorless pledget and the white background of the dacron swab make the identification of the organisms on the swab clear and distinct, just as it is with the exudate directly from a patient.  
 While some prior art, US. Pat. No. 3,450,129, Avery et al., makes use of frangible ampuls for carrying rea-,  
 gents, the use of that method is directed specifically to transporting a specimen to a laboratory and using their method for the purpose of preserving the viability of the bacteria until they can be cultured, whereas this is a diagnostic system and does not require culture. It is a direct test. Diagnosis is made at the time of collection of the specimen.  
  While only a few examples of my diagnostic reagent system and reagents have been described in detail herein, various changes and modifications may be made without departing from the scope of the inven-&#39; tion.  
 I claim:  
  1. A method for rapid testing for Neisseria gonorrhea in the male patient without culture and without gramstaining of this organism, consisting essentially of sampling the urethral exudate of a male patient directly onto a sterile swab to thereby provide a specimen of exudate on said swab; bringing said swab into contact with a porous absorbent pledget in dry condition which has been impregnated with a sole color-forming reagent selected from the group consisting of p-Amino Dimethylaniline Oxalate, N, N-Dimethyl-p- Phenylenediamine Dihydrochloride, N, N-Dimethyl-p- Phenylenediamine Oxalate, N, N-Dimethyl-p- Phenylene diamine Monohydrochloride and N, N, N N&#39; Tetra-Methyl-p-Phenylenediamine Dihydrochloride, which has been placed in a tube of transparent plastic having flexible side walls and a closed end; providing a frangible ampul in said tube containing physiological salt solution at pH 6.5 to 7.2 as a wetting activating agent for reagent in said pledget which is located adjacent said pledget at the end of said tube; said impregnated pledget in the dry state being positioned above said frangible ampul and being substantially colorless before said ampul has been broken; and manually squeezing said tube at the flexible side walls near the closed end adjacent said frangible ampul to break and to release said wetting activating agent thereby wetting said pledget and swab with specimen with said liquid wetting activating agent to produce a color change on the specimen of exudate on the swab when Neisseria gonorrheae is present, the color resulting from contact between said specimen on said swab wetted by said wetting activating agent which transports the reagent from the pledget to the swab reacting with the Neisseria gonorrheae present in the specimen and producing a color change normally to purple, redorange or dark grey. indicating a positive test of Neisseria gonorrheae in said specimen, the fragmented material from the ampul being retained within said tube and the color being observed within 30 to 120 seconds.  
  2. A method as claimed in claim 1 wherein said dry substantially colorless pledged disposed in said tube is impregnated with N, N, N N Tetra Methyl-p- Phenylenediamine Di-hydrochloride and said color change is from colorless to purple.  
  3. A method for rapid testing for Neisseria Gonorrheae in the male patient without culture and without gram-staining of this organism, consisting essentially of sampling the urethral exudate of a male patient directly onto a sterile swab to thereby provide a specimen of exudate on said swab; bringing said swab into contact with a porous absorbent pledget in dry condition which has been impregnated with a sole color-forming reagent selected from the group consisting of p-Amino Dimethylaniline Oxalate, N, N-Dimethyl-p- Phenylenediamine Dihydrochloride, N, N-Dimethyl-p- Phenylenediamine Oxalate, N. N-Dimethyl-p- Phenylenediamine Monohydrochloride and N, N, N N Tetra-Methyl-p-Phenylenediamine Dihydrochloride, which has been placed in a tube of transparent plastic having flexible side walls and a closed end; providing a frangible ampul in said tube containing a wetting activating agent consisting of water at pH 6.5 to 7.2 which is located adjacent said pledget at the end of said tube; said impregnated pledget in the dry state being positioned in contact with said frangile ampul and being substantially colorless before said ampul has been broken; and manually squeezing said tube at the flexible side walls near the closed end adjacent said frangible ampul to break and release said water at pH 6.5 to 7.2 thereby wetting said pledget and swab with specimen to produce a color change on the specimen of exudate on the swab when Neisseria gonorrheae is present, the color resulting from contact between said specimen on said swab wetted by water which transports the reagent from the pledget to the swab reacting with the Neisseria gonorrheae present in the specimen and producing a color change normally to purple, redorange or dark grey, indicating a positive test of Neisseria gonorrheae in said specimen, the fragmented material from the ampul being retained within said tube and the color being observed within 30 to seconds.