Certain 2-phosphorylimine-4-thiazolines used as insecticides and acaricides
United States Patent Mihailovski Apr. 8, 1975 [75] Inventor: Alexander Mihailovski, Berkeley,  
 Calif.  
 [73] Assignee: Stauffer Chemical Company, New  
 York, NY.  
 [22] Filed: June 10, 1974 [21] Appl. No.: 478,026  
  Related U.S. Application Data [60] Continuation of Ser. No. 184,205, Sept. 27, 1971. abandoned. which is a division of Ser. No. 23,051, March 26, 1970, Pat. No. 3,694,451.  
 [52] US. Cl. 424/200 [51] Int. Cl A0ln 9/12 [58] Field of Search 424/200 [56] References Cited UNITED STATES PATENTS 3.053.847 9/1962 Schickc 260/306,? 3.428.645 2/1969 Greco 260/306] Primary E.\&#39;aminerA-lbert T. Meyers Assistant Examiner-Leonard Schenkman Attorney, Agent, or FirmDaniel C. Block [57] ABSTRACT Compounds corresponding to the formula wherein X is oxygen and sulfur; R is lower alkyl, lower alkoxy, lower alkylthio and chloro; R is lower alkyl, lower alkoxy, lower alkylthio and chloro; R is lower alkyl, benzyl, aryl, power alkoxyalkyl, alkenyl, chloroalkenyl and alkynyl; Q is in which R and R are independently hydrogen. lower alkyl, halogen and nitro are used as insecticides and acaricides.  
 5 Claims, No Drawings CERTAIN 2-PHOSPHORYLIMINE- l-THIAZOLINES USED AS R S -Q wherein X is oxygen and sulfur; R is lower alkyl, lower alkoxy. lower alkylthio and chloro; R is lower alkyl. lower alkoxy. lower alkylthio and chloro; R is lower alkyl. aryl. lower alkoxyalkyl, alkenyl, chloroalkenyland alkynyl; Q is in which R and R are independently hydrogen. lower alkyl. halogen. and nitro.  
  The compounds of the instant invention are prepared according to the following general description of the synthetic procedure. Compounds of the form:  
  inf 2 are treated with an appropriate R -halide in a polar solvent (e.g.. methanol or ethanol) at room temperature or at elevated temperature as required. Potassium iodide may be used as a catalyst for the less reactive halides. The products from this reaction have the general structure Q N C=NH -ha11de (2) The free imino base is obtained after dissolving the imino salt (2) in water and adding one equivalent, or more, of strong base (e.g., sodium hydroxide solution).  
  Either the iminium salt (2) with two molar equivalents of base as acid acceptor or the free imine base in molar excess or with one molar equivalent of another base is phosphorylated using an alkoxy chlorothionophosphate (-phosphonate) in an inert solvent. This reaction is exothermic and the reaction temperature is held below 45C. by cooling. Bases which can be used as acid acceptors are sodium hydroxide, potassium carbonate, potassium hydrogen carbonate or tertiary amines such as triethylamine. Suitable solvents are acetone, methylene chloride, benzene or heterogeneous mixtures such as benzene/water. The final product isolates have the general structure wherein R is lower alkyl.  
  The thionophosphor (-phosphon) amides (4) are rearranged to thiolophosphor- (thiolophosphon-) amides (5) by dissolving them in an inert solvent (e.g.. benzene, benzene/hexane) and refluxing with alkyliodide (e.g., methyliodide, ethyliodide) or by refluxing them directly in the alkyl iodide. The products are purified by evaporating the solvent and alkyl iodide reagent. redissolving the crude product in a non-polar solvent such as benzene to precipitate the polar. solid byproduct usually accompanying the rearrangement. The benzene solution is filtered and the solvent removed to give the final product which can be still purified by recrystallization or chromatography. The products have the general thiolo structure (5).  
 wherein R is lower alkyl.  
  In the above formulas (l), (2), (3), (4) and (5). Q is l CH  A l-liter four-neck round bottom flask was charged with 40.0 grams (0.166 moles) 2-imino-3-methyl-4- thiazoline hydrogen iodide, 200 ml. benzene. and 53.0 g. (0.332 equivalents) 25% (w/w) sodium hydroxide solution. To this mixture were added dropwise with stirring and cooling 32.3 g. (0.166 moles) 0.0-diethyl chlorothiophosphate dissolved in 60 ml. benzene. The reaction mixture was stirred at ambient temperature for a further 3 hours. The benzene layer was separated and the aqueous phase extracted twice with 50 ml. portions of benzene. The combined benzene fractions were washed twice with 50 ml. portions of 5% NaH- CO dried and the solvent was evaporated to leave a tan-colored solid. This was recrystallized from petroleum ether/benzene. Some 34.0 g. of product were obtained, m.p. 4446C. Yield. 77% of theory.  
 Anal. Calcd. for C H N O PS C. 36.08; H, 5.68;  
 Found: C. 35.99; H, 5.75; N. 10.92; S, 24.07  
  The infrared spectrum of this compound (KBr pellet) showed a strong, broad band at 1.580 cm vibration. and strong bands at 1,040 cm&#34;, 1.020 cm and 780 cm (P-O-C stretch).  
  The proton magnetic resonance spectrum showed a triplet centered at 81.33 for six methyl protons of the ethoxy groups (1, 7H2), a singlet at 83.47 for three N- methyl protons. a doublet of quartets centered at 84.1 1 for four methylene protons of the ethoxy groups (1, 7H2; .1 9Hz), and a multiplet between 86.25 and 86.45 for one unsaturated proton adjacent to the ring sulfur atom and a multiplet between 86.70 and 86.90 for one unsaturated proton next to the ring nitrogen SCH wherein Q is -CII=( I- Compound Number In a solvent system consisting of 30 ml. benzene and 40 ml. n-hexane were dissolved 11.6 g. (0.0436 moles) 2-(0.0-diethylthiophosphorylimino)-3-methyl-4- thiazoline and 18.6 g. (0.131 moles) methyliodide. This solution was refluxed for 2 /2 hours. The volatiles were evaporated and the residual oil was dissolved in 100 ml. benzene. On standing at room temperature overnight, a small amount of solid precipitated. The solution was filtered and the solvent evaporated giving 10.3 g. of a pale-yellow. viscous liquid. Yield. 89% of theory.  
 Anal. Calcd. for C H N O PS C, 33.32; H, 5.19;  
 Found: C. 33.77; H. 5.36; N, 10.65; S. 25.47  
  The infrared spectrum (liquid film) showed a strong, broad band at 1,555 cm&#34; vibration), a strong band at 1,220 cm (P=O), and a strong band at 1.040 cm and a medium intensity band at 780 cm (P-O-C stretch).  
 The proton magnetic resonance spectrum of this compound displayed a triplet centered at 81.39 for the three methyl protons of the ethoxy group (.I 7H2), a doublet centered at 82.27 for three thiomethoxy protons (Jup l3Hz). a singlet at 83.48 for three N-methyl protons, a multiplet between 83.9 and 84.5 for two methylene protons of the ethoxy group, a doublet of doublets for one unsaturated proton, next to the ring sulfur atom, centered at 86.38 (J 5H2; 1, 1.4Hz) and a doublet of doublets centered at 86.82 for one unsaturated proton adjacent to the ring nitrogen (1,  
 Jup  
  The other compounds are prepared in an analogous manner starting with the appropriate reagents as outlined hereinabove.  
  The following is a table of compounds representative of those embodied in the present invention. Compound numbers have been assigned to them and are used for identification throughout the balance of the specification.  
 was;  
  5 TABLE 1 fCont&#39;d. Compound Number &amp; El 2 3 C H O CH S 4 O C H O C H S 5 S 6 1-1 0 C H O 6 S CH O CH O 7 0 CH O (111 8 8 S C11 0 CH 0 9 O C11 0 CH S 10 S CH 0 C11 0 l1 0 C11 0 (H 5 12 S C11 0 CH O 13 0 C H O C H O l4 0 CH 0 CH S 15 S CH O C11 0 16 O CH O CH S 17 S CH O CH O l8 &#34;0 ca o 011 s 19 S CH 0 CH O 20 O CH 0 CH S 21 S C H C 11 0 22 O C H CH S 23 S C H C 1-1 24 O C H 0 Cl 25 0 CH 0 CH S Systemic Evaluation Test This test evaluates the systemic toxicity by root absorption and upward translocation ofthe candidate systemic compound. The twospotted mite. TeIranyc/zus urticae (Koch) and the bean aphid. Apliix fabue (Scop.). are employed in tests for systemic activity. Young pinto bean plants in the primary leaf stage are used as host plants for the twospotted mite. The pinto bean plants are placed in bottles containing 200 ml. of the test solution and held in place with cotton plugs. Only the roots are immersed. The solutions are prepared by dissolving the compounds to be tested in a suitable solvent, usually acetone. and then diluting with distilled water. The final acetone concentration never exceeds about l7r. lmmeho H H 2o H H CH CH=CH 3 CH CH=CH CH H CH=CCl 2 H CH=CC1 diately after the plants are placed in the test solution. they are infested with the test species. Mortalities are determined after seven days. The percentage kill is determined by comparison with control plants which are 0 placed in distilled water only. The LD-5O value is calculated using well-known procedures. LD-SO values for the two-spotted mite are found in Table ll under column 2SM-SYS.; and for bean aphid under column BAS.  
  Young nasturtium plants, Tropaeolum sp., are used as the host plants for the bean aphid. The host plants are transplanted into one pound of soil that has been treated with the candidate compound. immediately after planting in the treated soil the plants are infested with the aphids. Concentrations of toxicants in the soil ranged from 10 ppm per pound of soil downward until an LD-50 value is obtained. Mortality is recorded after 72 hours. Comparison is made with control plants placed in untreated soil.  
  Insecticidal Evaluation Tests The following insect species are subjected to evaluation tests for insecticidal activity.  
 I. Housefly (HF) Musca domestica (Linn.)  
 2. Lygus Bug (LB) Lygus hesperus (Knight) Aliquots of the toxicants, dissolved in an appropriate solvent, are diluted in water containing 0.002% of a wetting agent. Sponto 221-(a polyoxyether of alkylated phenols blended with organic sulfonates). Test 1 concentrations range from 0.1% downward to that at which 50% mortality is obtained. In the tests, for these species. 10 one-month old nymphs of the lygus bug are placed in a circular cardboard cage sealed on one end with cellophane and covered by a cloth netting on the other. Test concentrations for the lygus bug ranged from 0.05% downward to that at which 50% mortality was obtained. Each of the aqueous suspensions of the candidate compounds are sprayed onto the insects through the cloth netting by means of a hand spray gun. Percent mortality in each case is recorded after 72 hours, and the LD-50 values expressed as percent of toxicant in the aqueous spray is recorded. The results are in Table II under column LB.  
  The following procedure is used to test houseflies. A stock solution containing 0.1% by weight of the toxicant in an appropriate solvent is prepared. Aliquots of this solution are combined with l milliliter of an acetone-peanut oil solution in an aluminum dish and allowed to dry. The aliquots are selected to achieve desired toxicant concentration ranging from 0.1% per Petri dish to that at which 50% mortality was attained. The Petri dishes are placed in a circular cardboard cage. closed on the bottom with cellophane and covhouseflies are introduced into the cage and the percent mortality is recorded after 48 hours. The LD-SO values are expressed in terms of percent concentration or pg per 25 female flies. LD-SO values obtained in the above-mentioned housefly test are found in Table 11 under column HF.  
  Systemic Test Salt-Marsh Caterpillar Esligmene acrea (Drurg) Aliquots of the toxicant dissolved in appropriate solvent are diluted in water and placed in glass bottles. Concentrations of active ingredient range from 10 ppm to that at which 50% mortality is obtained. Kidney beans, Pliaseolus vulgaris, supported by cotton plugs. are inserted into the solution so that the roots and major portion of the stem are completely immersed. Salt-marsh caterpillar egg masses (10 to 30 eggs) on small pieces of tissue paper are pinned to the kidney bean leaves and allowed tohatch. Mortality is recorded after five days and the LD-50 values are expressed as parts per million (ppm) of toxicant in the aqueous solution. The values are found in Table 11 under the column SMC-SYS.  
  The compounds are also active against two-spotted mite (Z-SM), Terranyclius um&#39;cae (Koch). Lima bean plants. Phaseolus sp.. are utilized as the host plant and infested with 50 to 75 mites of various ages. Twentyfour hours after infestation they are sprayed to the point of run off with aqueous suspension of the toxicant. Test concentrations range from 0.05% to that at which 50% mortality is obtained. The values obtained in this test are found in Table 11 under the columns 2SM-PE and ZSM-EGGS.  
  The compounds are also active against bean aphid, Aphis fabae (Scop.), as a contact toxicant. The same test procedure as given for the two-spotted mite above is used for the bean aphid except nasturtium, Tropaeolum sp., plants approximately 2 to 3 inches tall are used as the host plant. The LD-50 values obtained for the compounds of this invention are found in Table II cred on top with cloth netting. Twenty-five female 40 nde umn TABLE II Insecticidal Evaluation Results LD-50 Values SMC Z-SM Com- HF LB BA BAS SYS PE EGGS SYS pound Number (pig/25 Q) (71) (7:) (ppm) (ppm) (71) (pp 1 100.0 .05 .05 .8 .03 .05 l0.0 2 50.0 .003 .003 .8 100 .03 .03 3.0 3 45.0 .003 .03 .3 l0.0 .01 .01 8.0 4 90.0 .003 .05 .03 .03 8.0 5 100.0 .05 .05 .05 6 100.0 .03 .05 .05 10.0 .05 .05 3.0 7 70.0 .001 .01 .03 10.0 .03 .05 8 30.0 .05 .05 .03 .05 8.0 9 10.0 .003 .03 3.0 l0.0 .03 .03 8.0 10 l00.0 .05 .05 .05 11 100.0 .05 .05 .05 12 100.0 .05 .05 .05 I3 I00.0 .05 .05 .05 14 75.0 .01 .03 l0.0 .05 .05 .8 15 100.0 .05 .05 .05 .05 .8 16 100.0 .05 .05 .05 .05 l7 100.0 .05 .05 .05 18 100.0 .05 .03 .05 l9 l00.0 .05 .05 .05 20 100.0 .05 .05 .05 21 100.0 .05 .05 10.0 .05 .05 22 50.0 .003 .0003 .8 .8 .008 .03 3.0 23 100.0 .05 .05 .05 .05 24 100.0 .05 .05 .05 25 l00.0 .05 .05 l0.0 .03 .03 3.0  
  9 10 What is claimed is: $=C- 1. A method of killing insects and acarids comprising g applying to the habitat thereof an effective amount of 4 a compound corresponding to the formula:  
  V 5 wherein R and R are hydrogen. &#39;i 2. The method of claim I wherein R is methoxy and T/P-N:CITNTQ-S R3 iS 3. The method of claim 1 wherein R is ethoxy and R is methyl. 4. The method of claim 1 wherein R is methoxy and R is propynyl.  
  5. The method of claim 1 wherein R is ethyl and R is methyl.  
 wherein X is oxygen; R, is independently selected from the group consisting of methoxy. ethoxy and ethyl; R is methylthio; R is independently selected from the group consisting of methyl and propynyl; and Q is