7-Azaindole fused heterocyclic compounds
United States Patent H9! Ledig et al.  
 [ Apr. 29, 1974 7-AZAINDOLE FUSED HETEROCYCLIC COMPOUNDS [75] Inventors: Kurt W. Ledig, Philadelphia; David R. Herhst, Wayne. both of Pa.  
 [73] Assignec: American Home Products Corporation, New York, NY.  
 [22] Filed: Apr. 25. 1974 [2l] Appl, No: 464,077  
 Related US. Application Data [63I Continuationin-part of Scr. No 351.375 April 18. [973, which is a continuation-in-part of Scr. No. 248.999. Ma 1, I972, abandoned,  
 [52] US. Cl 260/296 P; 260/293531260/297 F: 424/2631424/267 [51] Int. Cl C(I7d 57/04 Field of Search 260/2935}, 296 P, 297 F [Sol References Cited UNITED STATES PATENTS l/l J&#39;ll Davis 260/243 l H1973 Herhst fit ill. loll/293.53  
 / \V l N l N i I (,H  
  3 R CIL. 8  
 and  
 l O I l N GILL O CH 5 cm I.)  
 where R is hydrogen, alkyl of from 1 to 7 carbon atoms, or allyl; R is methyl, ethyl, or n-propyl; R is alkyl of from I to 7 carbon atoms or ally]; R&#34; is hydrogen or methyl and n is l or 2. or the pharmacologically acceptable acid addition salts thereof have analgesic activity.  
 8 Claims, 1 Drawing Figure 7-AZAINDOLE FUSED HETEROCYCLIC COMPOUNDS CROSS-REFERENCE TO COPENDING APPLICATION This application is a continuation-in-part of pending application. Ser. No. 352.375. filed Apr. 18. I973. which is a eontinuation-in-part of application. Ser. No. 248.999. filed May I. I972. now abandoned.  
 BACKGROUND OF THE INVENTION This invention relates to compounds classified in the art of chemistry as substituted 2.3.5.6.1 l.l lb-hexahydro- I H-pyridol 3 2 &#39;:-l.5 lpyrrolol 3.2-glindolizines and l.Z.3.-l.n.7.lllZb-octahydropyrido[3&#39;. Z&#39;:4.5lpyrrololZJ-alquinolizines. to intermediates thereto. to methods for using such cotnpounds. and to pharmaceu&#39; tical compositions thereof.  
  In Herbst et al. J. Med. (lit-ML. 9. 864 (1966) the compound 2.3.5.61 l.l lb-hexahydro-l l-methyl-IH- indolizinol3.7-blindole is disclosed. In US. Pat. No. 3.478.05 l there are disclosed compounds described as 2.3.5.6.l l.l lb-hexahydro-l lb-alkyl (and phenyll-3B; indolilinol8.7-blindoles and the 3-oxo derivatives thereof. and the l3.3.4.611lllb-octahydro-llbalkyl (and phenyl l indolol 2.3-alquinolizines. and the 4 l lfll-oxo derivatives thereof.  
 SUMMARY OF THE INVENTION The invention sought to be patented in a principal composition aspect resides in the concept of a chemical compound of the formulaei and Ic It] wherein R is hydrogen. alkyl of from I to 7 carbon atoms. or allyl; R is methyl. ethyl. or n-propyl; R is alkyl of from I to 7 carbon atoms or allyl; R is hydrogen or methyl and n is I or 2. and the pharmacologically acceptable acid addition salts thereof.  
  The tangible embodiments of the principal compositions represented by Formulae la and lc possess the m herent general physical properties in the free base form of being colorless oils or solids. substantially insoluble in water and generally soluble in organic sobents such as benzene. methanol. chloroform. and acetone. In the form of their acid addition salts they are generally white solids. appreciably soluble in water and methanol. The compounds represented by Formula lb and Id as their acid addition salts are solids and are substantially soluble in water.  
  Examination of the compounds of Formulae la and lc produced according to the hereinafter described processes reveals upon infrared. ultraviolet. and nu clear magnetic resonance spectrographic analysis. spectral data confirming the molecular structure here inbefore set forth. The aforementioned physical characteristics taken together with the nature of the starting materials. the elemental analyses. and the mode of syn thesis. positively confirm the molecular structure hereinbefore set forth.  
 The tangible aspects of the principal compositions represented by Formulae la. lb. lc. and Id possess the inherent applied use characteristics of exerting analgesic effects in warm-blooded animals. as evidenced by pharmacological evaluation in standard test procedures.  
  Preferred embodiments of the compounds of Formula la and lb are those wherein R or R is alkyl of from I to 5 carbon atoms or allyl and R and n are as hereinbefore defined.  
  The invention sought to be patented in a second composition aspect rcsides in the concept of a chemical compound of the formulae:  
 wherein R is hydrogen. alkyl of from l to 7 carbon atoms. or allyl; R&#39; is methyl. ethyl. or n-propyl: R is hydrogen or methyl and n is l or 2; and the acid addition salts thereof.  
 The tangible embodiments of the compositions represented by Formulae Ie and If possess the inherent general physical properties in the free base form of being colorless oils or solids. substantially insoluble in water. and generally soluble in organic solvents such as chloroform and tetrahydrofuran. In the form of their acid addition salts they are generally white solids. appreciably soluble in water and methanol.  
  Examination of the compounds of Formulae le and If produced according to the hereinafter described processes reveals upon infrared. ultraviolet. and nuclear magnetic spectrographic analysis. spectral data confirming the molecular structure hereinbefore set forth.  
 The aforementioned physical characteristics taken to- 40 gether with the nature ofthe starting materials. the elemental analyses. and the mode of synthesis. positively confirm the structures of the compositions sought to be patented.  
  The compounds of Formula le possess the inherent applied use characteristic of being intermediates for the preparation of the analgesic compounds of Formula la or Formula lb. The compounds of Formula If possess the inherent applied use characteristic of being intermediates for the preparation of the analgesic compounds of Formulae lc or id.  
  The invention sought to be patented in a third composition aspect resides in the concept of a composition suitable for administration to a warm-blooded animal comprising:  
 a. a compound of the formulae:  
 l&#39; N N l 2 CH H CH and on ca 011 wherein R is hydrogen. alkyl of from l to 7 carbon atoms, or allyl; R is methyl. ethyl. or n-propyl; R is alkyl of from 1 to 7 carbon atoms or allyl; R is hydrogen or methyl and n is l or 3. or the pharmacologically acceptable acid addition salts thereof; and  
 b. a pharmaceutically acceptable carrier.  
  The invention sought to be patented in a process aspect resides in the concept of a method for producing analgesia in warm-blooded animals which comprises administering to a warmblooded animal in need thereof. an amount sufficient to produce analgesia in said warm-blooded animal of a compound of the formulae:  
 and I en 3 ca- Id Q wherein R is hydrogen. alkyl of from I to 7 carbon atoms. or allyl; R is methyl. ethyl, or n-propyl; R is alkyl of from 1 to 7 carbon atoms or allyl; R is hydrogen or methyl; and n is l or 2. or the pharmaeologically acceptable acid addition salts thereof.  
 DESCRIPTION OF THE DRAWING In describing the invention herein. reference will be made to the annexed Drawing. which illustrates schematically the reaction sequence for preparing the 2.3.- 5.6.l l.l lb-hexahydrolfl-pyridol 3&#39;, 2&#39;:4.5 lpyrrolo[3.2-g1indolizines. the compounds of Formula la. wherein n is l and R and R have the meanings hereinbefore defined.  
 DESCRIPTION OF THE PREFERRED EMBODIMENTS Referring now to the Drawing. wherein the compounds are assigned Roman numerals for identification. in Process A. 7-azatryptamine (II) is treated with a keto acid of the formula:  
 to yield the keto amide intermediate (ill) and then cyclizing said intermediate. The first step in Process B is carried out by a method similar to that described by F. Shiroyan. in Arm. K/rim. 2/1.. 20. 649 I967). The cyclization is effected by heating the keto amide intermediate (III) in the presence of mineral acid. for instance hydrogen chloride in isopropanol.  
  The aminoamide intermediate (IV) is converted to the amine (V). in Process C by reducing the amide carbonyl with a reducing agent such as lithium aluminum hydride. The reduction is performed in a non-reactive solvent. such tetrahydrofuran. at reflux temperature. Alternatively. the reduction can be carried out by converting the ketoamide to a thioamide by contacting with phosphorous pentasulfide in a non-reactive solvent and then reducing the thioamide with Raney nickel using methods similar to those described in US. Pat. Nov 3.454.583.  
  N,-,,,,-Alkylation of the amine (V) affords the product (Vll The alkylation is accomplished by contacting the amine (V) with a base in a non-reactive solvent. eg .sodium hydride in dimethyl formamide. and then adding an alkylating agent. e.g. a lower alkyl halide or tosylate. at a temperature ranging from about 0 to about 100C. When R is lower alkyl. the product (Vll) may also be prepared in Process D by N alkylation of the aminoamide (IV) followed by reduction of the amide carbonyl function using the reduction methods heretofore described.  
  It will be appreciated that the indolic nitrogen atom of 7-azatryptamine may be substituted with an appropriate alkyl group (R). Such substituted compounds may be employed as starting materials in Process A of the above-described method to give the aminoamide (Vll) directly without the need of the alkylation step (Process D).  
  The amine (V) may also be prepared by the Fischer indole synthesis by reacting a keto compound of the formula:  
 wherein R&#39; is methyl. ethyl. or n-propyl. with 2- hydrazinopyridine or an N-(alkyl)-N-(2- pyridylJhydrazine. The keto compound (Vlll) may be synthesized according to the following methods:  
  A Z-(alkyhproline (IX) is first con erted to its methyl ester (X) using standard procedures. e.g. thionyl chloride and methanol. If the ester is obtained as an acid addition salt (which will be the case when the thionyl chloride-methanol method is employed). it must be neutralized with a strong base. e.g. triethylamine. to afford the free amine. The free amino ester (X) is then Nailkylated with an appropriate methyl 4-halobutyrate to afford a diester (Xl) which is then cyclized in the presence of a base. e.g. sodium methoxide. to yield in situ a B-keto ester compound which is extracted into water. The water extract containing said intermediate is acidified to about pH l and heated. whereupon hydrolysis and decarboxylation takes place to give the kcto compound (VIII).  
  The l.2.3.4.6.7,l2.l2b octahydropyridol3&#39;,2:4.5]pyrrolo [2.3-alquinolizine compounds of Formula la (ie those where n is 2) may be prepared as in Process A or Process B as previously described. from 7-azatryptamine. or an N ,,,,-(alkyl)- substituted 7-azatryptaminee. and a keto acid:  
  l n n R -CCll CH ClI COll or an enol lactone:  
 where R is methyl. ethyl. or n-propyl.  
 The cyclic intermediate:  
 thus formed is converted to a compound of Formula (la) where n is 2. using the reduction and N alkylation procedures hereinbefore described. or obvious modifications thereof.  
  The compounds of Formula lc, where R is hydrogen or methyl. can be prepared from 7-azatryptamine and Z-methyllevulinic acid using Process A. as abovedescribed. and employing the reduction and N alkylation procedures hereinbefore described.  
  The compounds of Formula la. lc. le or has obtained in the above-described processes and appropriate intermediates thereto may be isolated and purified in a com ventional manner. It is furthermore appreciated that in the various processes described above. factors such as solvents and temperatures are not critical. In general.  
 the sol\ent selected must be rcactioninert. i.e. it must not interfere with the course of the reaction. and be such that the reactants are soluble therein. When a final product or intermediate is obtained as an acid addition salt. it may be converted to the free base by conventional methods. such as by dissolving the salt and treating an appropriate solution with sodium hydroxide. hen a final product or an intermediate is obtained as a free base. it may be converted to an appropriate acid addition salt by dissolving the base and treating the solution with the desired acid.  
  The starting materials for the above-described synthetic procedures are described in the literature or may be prepared by procedures well known in the art. 7- Azatryptamine is described by M. Robinson and B. Robinson. J. Am. (hear 5mg. 78. 1247 llSb); French Patent 1.26: LU): British Patent 897.988; and US. Pat. No. 3.354174. Z-Methylproline is described by K. ls&#39;ariyone. Client. and Pliurm. Hull. (Japan) 8. Hit) (Wot)! and in US Pat. No. 3.412110.  
  The N-oxides of Formula lb. where R. R and n are as hereinbefore defined. are prepared by oxidation of a compound of the formula:  
 where R is alkyl of from 1 to 7 carbon atoms or allyl; R is methyl. ethyl. or npropyl. or u is I or 2&#39;. with an organic peroxyacid. Suitable peroxyacids are peroxyformic. peroxy&#39;acetic. peroxytrifluoroacetic. chloroperoxybenzoic. peroxyphthalic. and the like. m- Chloroperoxybenzoic acid is. however. preferred. The peroxyacid may be either preformed or prepared in situ by the interaction of hydrogen peroxide with an appropriate carboxylic acid. as described in Fieser and Fieser. Reagents For Organic Synthesis. John Wiley. 1967. pp. 451465.  
  in general. the oxidation reaction is performed in a reaction-inert organic solvent at a temperature ranging from about 5 to about 50C for a period of time up to 1 hours. The preferred temperature range is from about -5 to about 5C. and the preferred solvent is tet rahydrofuran. The N,,-oxide derivative will form an acid addition salt with the carboxylic acid formed by the decomposition of the peroxyacid selected. The salt formed thereby may be recovered. or it may be converted to a salt containing an anion other than that contained in the salt formed initially by contacting the salt with an organic or mineral acid having an acid strength greater than that of the carboxylic acid to which the N,,- oxide derivative was initially bound. For instance, when the oxidizing agent is m-chloroperoxybenzoic acid. the N,,-oxide derivative combines with m-chlorobenzoic acid. The m-chlorobenzoic acid addition salt can be contacted with hydrogen chloride to afford the hydrochloride salt.  
  lf desired. the N,,-oxide derivative as the acid addition salt may be converted to the corresponding free N,,-oxide derivative by contacting the salt with an ap propriate base. If the neutralization is performed in a suitable. pharmaceutically acceptable solvent and if no toxic products are produced. the solution of the N oxide free base can be used directly for the biological purposes herein described. For example. the salt may be dissolved in water and treated with sodium hydro ide in an amount sufficient to generate the base.  
  The bi -oxide of Formula ld can be prepared in a manner as hereinbefore described for the preparation ol&#39;the compounds of Formula lb by the reaction of 2.3.- 5.b.l l.l lb-hexahydro-ll l.l lb-trimethyl-lflpyridol3&#39;.2:4.5Ipyrroloi3.3-glindolizine with an appropriate peroxidizing agent.  
  Since the compounds of the invention possess an asymmetric carbon atom. optical enantiomorphs are possible. and the compounds of the invention may be pure enantiomorphs or mixtures of such enantiomprphs. such as the racemates. The products of the invention may be obtained in the form of the pure enantiomorphs either by carrying out a resolution at any convenient stage of the synthesis of the compounds or by resolving the desired products. The resolutions may be carried out by methods known in the art. For exam plc. the pure dextro and levo enantiomorphs of 2.3.5.6- l.l lb-hexahydro-l l.l lb-dimethyl- [Llpyridol 3 &#39;.2:4.5l pyrrolol ll glindolizine are obtained by treating the racemate with 2.3-dibenzoyl- 2( Rjz3t R l-tartaric acid. The salt of the l-isomer is isolated by crystallization. and the l-isomer in its free base form is obtained by treating the salt with a suitable strong base. The d-isomer is isolated by treating the base obtained upon basifying the mother liquor front the above crystallization step. with 2.3-dibenzoyl- 2(S):3(S)-tartaric acid. crystallizing the salt formed thereby. and treating the salt with a suitable strong base. If desired. the resolved bases can be converted to the corresponding acid salts by conventional proce dures. The pure cnantiomorphs of the bases can be subjected to oxidation with a peroxyacid. For example dextro-2.3.5.6.l1.1lb-hexahydro-llb-dimethyl-lfipyrido[3,2&#39;:4.5]pyrrolo{3.2-glindolizine 4-oxide. dihydrochloride can be prepared by the oxidation of dextro-2.3.5.6.l l.l lb-hexahydro-l l.l lb-dimethyl-lflpyridol3&#39;.2&#39;:4.5]pyrrolo [3.2-g1indolizine.  
  As employed herein. the term dextro&#34; (d) denotes the isomer which has the property of rotating the plane of polarized light in a positive direction. as measured by conventional polarimetric procedures. so that the specific rotation [01],, of the compound being measured has a positive value. The term lev0 l denotes the isomer which has the property of rotating the plane of polarized light in a negative direction so that the specific rotation has a negative value.  
  The analgesic activity of the compounds of Formula la. lb. and le has been demonstrated in rats according to the method of DAmour and Smith. J. P/wrmucol.. 72. 74 194i the details of which are given herein in Example XXXVI]. The compound, 2.3.5.6.] 1.] lbhexahydro-l l.l lb-dimethyl-lfl-pyrido l3&#39;,2&#39;:4.5]pyrrolo[3.2-g1indolizine has been found to be an extremely active analgesic. the potency being about I It) times greater. orally. and about [7 times greater. intramuscularly. than that of morphine.  
  ln practicing the process aspects of inducing analgesia in warm blooded animals. the effective dosage will vary according to the particular compound being employed. the severity and nature of the pain. and the particular subject being treated.  
  In general. with large warmblooded animals (about 70 kg body weightieffective results can be achieved by the oral route at a dosage le\el of from (it to about 30 mg given every 4 hours or as needed. By the intramuscular route. effective results can be achieved at a this age level of from 0.5 to about 7.5 mg as needed. Therapy should he initiated at lower dosages. the dosage thereafter being increased until the desired analgesia is obtained. For 2.3.5.6.] 1.1lb-hesahydro-l 1.1 lbdimethyll fl-pyrido [3 2 &#39;:-1.5 lpyrrolol 3.2- glindolizine. a preferred embodiment. the preferred range is 0.1 to 1.0 by the oral route and 0.5 to about 2.0 mg by the intramuscular route.  
  When employed as analgesic agents. the active substances may be administered alone or in combination with pharmaceutically acceptable carriers. the proportion and nature of which are determined by the solubility and chemical properties of the compound selected. the chosen route of administration, and standard pharmaceutical practice. For example. the compounds may be administered orally in solid dosage forms. eg capsules. tablets. or powders. or in liquid forms. eg. solutions or suspensions. The compounds may also be injected parenterally in the form of sterile solutions or suspensions. Solid oral forms may contain conventional excipients. l&#39;or lltSllmCCi lactose. sucerose. magnesium stearate. resins. and like materials. Liquid oral forms may contain various flavorings. coloring. preserving. stabilizing. buffering. solubilizing. or suspending agents. lf desired. additives. such as saline or gluclose may be added to make the solutions isotonic.  
  For parenteral administration. it is convenient to employ the compounds o1 the invention in the form of their pharmacologically acceptable acid addition salts. which are water soluble and thus may be readily incorporated into preparations suitable for injection. The salts are prepared by methods well known in the art. Appropriate salts are those formed from both inorganic and organic acids. for example: hydrochloric. hydrobromic. sulfuric. sulfonic. phosphoric. nitric. maleic. fumaric. benzoic. ascorbic. pamoic. succinic. methanesulfonic. acetic. propionic. tartaric. citric. lactic. malic. mandelic. cinnamic. palmitic. itaconic. and benzenesulfonic.  
  The following examples are illustrative of the processes of the invention All temperatures are in centri grade. The rotation values in Examples XXV and XXVI were determined using a Zeiss LEP A2 polarimeter (0.5 dm. cell) by extrapolation of readings taken at 578nm and 546 nm.  
 EXAMPLE 1 1.2.5.6.1l.llb-Hexahydro&#39;1lb-Methyl-flfl-Pyrido [3,2&#39;:4.51Pyrrolol 3.2-g]lndolizin-3-One Method l Butyl cellosolve (70 ml). 2.9 g levulinic acid and 2.0 g 7-azatryptamine [M. M. Robinson and B. C. Robinson. J. Am. Chem. Soc. 78. 1247 (1956); French Patent 1.261.179 -C.A.. 57. 2274b 1962 British Patent 897.988 CA. 57. 12567d (1962)] are refluxed (under N for hours and the solvent is removed in vacuo. The residual gum solidifies upon trituration with water-excess aqueous sodium hydroxide and the brown solid is collected. thoroughly washed with water and triturated with ether. Recrystallization (twice) of the crude product from acetone yields 0.7 g title compound. dec. 253-254: A,.,,, 3.31 5.97 a; NMR (CDCI I: 51.77 (singlet. llb-methyl). 4.58 (multiplet. C-S proton) ppm.  
 Method (2) 7-Azatryptamine (4.6 g). 3.1 g angelicalactonc and 60 ml dry tetrahydrofuran (THF) are refluxed for one hour and the solution is chilled at +5 for ca 16 hours. The solids are collected. washed with cold (ca THF and thoroughly dried to afford 4.6 of N-[2-(7- azaindol-3-yl )ethyl]-4-oxovaleramide. mp. l36137; A,,,,, 3.05. 5.86. 6.11 u: NMR(CDC1;.)52.17 (singlet. COCHM. 6.49 (multiplet. amide NH) ppm.  
  lsopropanol ml). 3.9 g. of N-[2( 7-azaindol-3-yl) ethylI-4-oxovalcrantide and 5 ml 3.2 N HCl/isopropanol solution are refluxed for 3 hours and the reac tion mixture then is evaporated to dryness. Successive rccrystallizations of the residue from isopropanol. chloroform-ether and isopropanol-methanol provide 17 g of the title compound as the hydrochloride salt solvated with 0.2 molecule isopropanol per molecule of salt. dcc. 283286; A,,,,, 2.95. 4.05. 5.94 u; NMR (dDMSO): 81.05 (doublet. .l=6 Hz. isopropyl methyl). 1.65 (singlet. 11b methyl). 4.27 (multiplet. C5 proton) ppm.  
  The preceding hydrochloride (0.5 g. solvated with isopropanol) is converted to the free base which is recrystallized from acetone and from nitromethane. A 208 mg quantity of material is isolated. and a 101 mg portion is recrystallized from acetone to yield 64 mg title compound. dec. 255256. Taken simultaneously. the product of Method l decomposes at 254 while a mixture of the two specimens decomposes at 254.  
  The IR. NMR and UV spectra ofthe two samples are similar exhibiting only very minor differences arising from the presence of differing. trace amounts of solvent.  
 EXAMPLE ll 2.3.5.6.]1.1lb-Hexahydro-llh-Methyl-lfl-Pyrido l3&#39;.2&#39;:4.5]Pyrrolo[3.2-g]lndolizine Method 1 To 15 ml methanol at l0 are added. in turn. 3.2 ml thionyl chloride and 5.2 g d1-2-methylproline lK. Kariyone. Chem. and Pharm. Bull. (Japan). 8. 1110 (1960); US. Pat. No. 3.422.110] and the solution is kept at ca. 25 for 3 hours and then is refluxed for 2 hours. The solvent is removed and the residue is recrystallized from acetone to provide 3.2 g 2-methy1-2- pyrrolidine-carboxylic acid. methyl ester, hydrochloride. m.p. -96; )t,,,,,,&#34; 3.68. 5.73 41.; NMR (dDMSO): 51.63 (singlet. 2-methyl). 3.79 (singlet 0 methyl) ppm.  
  A mixture of the 63.0 g salt. prepared as above and 500 ml triethlamine is vigorously stirred for 4 hours and filtered. The filtrate is retained and the solids are triturated three times further with triethyl amine as before. Removal of triethyl amine from the combined filtrates yields 41.0 g crude 2-methyl-2-pyrrolidinecarboxylic acid. methyl ester A,,,,,,&#39; 3.00. 5.77 p.  
  Acetone (300 ml). 17.] g 2-methyl-2- pyrrolidinecarboxylic acid. methyl ester. 16.5 g anhydrous potassium carbonate. 18.0 g methyl 4- chlorobutyrate and 19.5 g anhydrous sodium iodide are stirred and refluxed for 48 hours. The reaction mixture is filtered and the filtrates are freed of solvent. Suspension of the residue in ether. filtration and evaporation of ether from the filtrate gives a residue which is dis tilled to provide 14.0 g of 2-carboxy2-methyl1 pyrrolidinebutyric acid. dimethyl ester. b.p. l05-108/0.1 mm; )t,,,,, 5.75 a; NMR (CDCI 61.25 (singlet. Lmethyl). 3.65 (singlet. O-methyls) ppm.  
 Analysis for -H M) Calculated, L&#39;. 59.24. H. 8.70: N. 5.70 Found: (1 59,01. H. 8112. N. 5.7!  
  Toluene (400 ml 8.9 g sodium methoxide and 33.3 g 2-carboxyQ-methyl-l-pyrrolidinehutyric acid. dimethyl ester are refluxed for 4 hours. The reaction mixture is extracted with water and the combined aqueous extracts (ca 300 ml) are made strongly acidic with concentrated HCl and then are heated on the steam bath. After 12 hours of heating a negative ferric chloride test is obser ed.  
  A 20 ml portion of the preceding acidic solution (neg. ferric chloride test) and 2.0 g 2- hydrazinopyridine. hydrochloride, are refluxed for hours and then are evaporated to dryness. The residue and ca. 6 g polyphosphoric acid are heated during successive one hour periods at 170, 200. and 250. After cooling. the reaction mixture is mixed with ice. basified with aqueous sodium hydroxide and is extracted with ether. The ethereal extracts are washed with brine. dried (magnesium sulfate) and freed of solvent to give an oil which is dissolved in dil. aqueous hydrochloric acid and washed with ether. Basiflcation of the acidic solution. extraction with ether and removal of solvent from the ethereal extracts provide 1.5 g oily base that is chromatographically purified on a column of neutral. activity lll alumina. From the chloroform eluates is isolated a crystalline solid which is recrystallizied from ether to yield 350 mg title compound. m.p. l99200&#34;; 3.16 [1; NMR (CDCl 51.65 (singlet. llb mruthlUH 95 t (59.460) my; )t,,,.,, 249 (e690) mp.  
 C. 73,97; H. 7.54; N. 18 49 73,99; H. 7.451N. 18.41  
 Method (2) A refluxing and stirred (under N suspension of 2.0 g lithium aluminum hydride in 100 ml dry THF is treated slowly with a solution of 4.0 g 1.2.5.6.1 l.l lbhexahydro-l lb-methyl-3flpyrido[ 3 &#39;.2 :4.5 ]pyrrolol 3 .2-glindolizine-3-one in 200 ml dry THF. Refluxing and stirring are continued for 2 hours longer and the reaction mixture is cooled as 10 ml N NaOH solution are added cautiously with vigorous stirring. The mixture is filtered and the solids are washed thoroughly with boiling THF. Removal of solvent from the combined THF fractions gives a residue which is dissolved in methanol and acidified with excess isopropanolic hydrogen chloride. Dilution of the acidified methanolic solution with acetone and chilling yield a crystalline solid. Recrystallization of this material from methanolacetone (thrice) provides 1.7 g title compound as a dihydrochloride salt. m.p. 280282; )t,,,,,,&#39; 2.99. 4.02 a; NMR (dDMSO): 52,02 (singlet. llb-methyl) ppm; h 223 (627.200). 290 (510.080) mu; x,,,,-,,-&#39;-&#34;&#39;&#34; 244 (51.280) mu.  
  An aqueous solution of ca. 100 mg of the dihydro chloride salt in water is basified with excess aqueous sodium hydroxide and the solution is extracted with ether After washing with brine. the ethereal extracts are concentrated to a volume of ca. 5 ml and chilled to provide. after drying. 60 mg title compound. m.p. 200. Basetlon the melting point. mixture melting point. thin layer chromatrographie comparisons and IR. NMR and UV spectral data. this product is identical with the diamine prepared by Method (1):  
 EXAMPLE 111 2.3.5.6.1 l.1lbHexahydro 1l.l lh-Dimethyl-IH- Pyridol 3&#39;.2&#39;:4,5 lPyrrolo[3.2-gllndolizine Method l To a solution of8.l g 1.2.5.6.1 l.l lb-hexahydro-l lbmethyl-Sfl-pyridol3&#39;,2&#39;:4.5]pyrrolo[3.2-glindolizin- 3-one in ml dry dimethylformamide (DMF) are added 1.8 g ca. 50 percent sodium hydride-mineral oil dispersion and the mixture is stirred for one hour, Methyl iodide (4,76 g) iii 10 ml dry DMF is added. stirring is continued for 2 hours and thc mixture then is freed of solvent (in vacuo). A solution ofthe residue in dilute aqueous hydrochloric acid is washed with ether and the acidic solution then is made strongly basic and is extracted with chloroform. The chloroform extracts are washed with brine. dried over magnesium sulfate and then the solvent is evaporated. Dissolution of the residual gum in a small amount of methanol. addition of excess isopropanolic hydrogen chloride and ether cause precipitation ofa salt which is recrystallized from methanol to give 3.8 g of 1.2.5.6.] l.l lbhexahydro- 11.11bdimethyl-3H-Pyrido[3&#39;,2&#39;:4.5)pyrido{3.2- gIindolizin-3-one as a hydrochloride salt bearing 0.25 molecule water per molecule of amino-amide hydrochloride. m.p. 272275&#34;; A,,,,,,&#34;&#39; 2.96. 4.42. 5.9] u; NMR (dDMSO): 61.62 (singlet. llb-methyl). 3.90 (singlet. ll-methyl). 4.30 (multiplet. C-5 proton) ppm.  
  Sodium methoxide (0.93 g) is added to a solution of 5.0 g 1.2.5.6.1l.llbhexahydro-llJlb-dimethyl-3 l-1- pyrido[3&#39;,2&#39;: 4.5]pyrrolo[3.2-g]indolizin-3-one. hydrochloride. 0.25 hydrate in ml hot methanol and the mixture is freed of solvent and the residue is thoroughly dried. The solids, suspended in 100 ml dry TH F. are added slowly to a refluxing and stirred suspension of 2.5 g lithium aluminum hydride in 100 ml dry THF. After a 2-hour reflux period. the reaction mixture is cooled. carefully treated with 12.5 ml N NaOH solution and filtered. The solids are thoroughly washed with boiling THF and the solvent is removed from the combined THF fractions. Dissolution of the residue in ether and addition of excess isopropanolic hydrogen chloride afford a precipitate which is collected and recrystallized from methanol to yield 2.50 g of title compound as the dihydrochloride salt. dec. 305&#34;; x,,,,,,&#34;&#39;&#34; 4.17. 4.90 NMR (dDMSO): 61.98 (singlet. llb-methyl). 3.87 (singlet. ll-methyl) ppm, A,,,,,,&#34;&#34; 225.5 (e25.600) 290 (68.970) mu; A,,,,-,, 248 (6660) Method (2) To a solution of 1.4 g 2.3.5.6.1Ll lb-hexahydro-l lbmethyllfi-pyridol3&#39;.2&#39;:4.5]pyrrolo[3.2-glindolizine (See Example ll) in 20 ml dry DMF is added 0.33 g of at ca. 50% sodium hydridemineral oil dispersion and the reaction mixture is stirred for 1 hour (under N Methyl iodide (0.96 g) is added and stirring is continued for an additional hour. Several drops of glacial acetic acid are added and the DMF is removed in vacuo. The residue. after dissolution in dilute aqueous HCl. is washed with ether and the acidic solution is made strongly basic and is extracted with ether. Washing of the ethereal extracts with brine. drying (MgSOn and removal of solvent produce 1,05 g title compound as a gum. NMR (CDCl;,): 51.60 (singlet. llb-methyl].  
 A 0.9 g portion of the above base in 50 ml ether is treated with excess isopropanolic HCl. The salt thus formed is washed with ether and thoroughly dried to yield 0.65 g title compound as the dihydrochloride salt. dec. 305. By 1R. NMR and UV spectral comparisons. this product is the same as the salt prepared by Method 1 1 above.  
 EXAMPLE lv&#39; 11-Ethyl-2.3.5.6.11.11b-Hexahydro-llb-Methyl-lfl- Pyridol 3.2:4.5 lPyrrolol3.2-gllndoli2ine Ethylation of 3.2 g of 2.3.5.6.] 1.11b-hexahydro-l1bmethyl-lfl-pyrido[3&#39;.2&#39;:4.51pyrrolo[3.2-g1indolizine with 0.76 g ca. 50 percent sodium hydride mineral oil dispersion and 2.4-1 g ethyl iodide is conducted in a manner similar to that of Example 111. Method 2. Benzene extracts of the basic aqueous fraction are washed with brine. dried (magensium sulfate) and placed on a 100 g column of neutral. activity 111 alumina. Elution of the column with 3/7 ether-benzene yields 22 g title compound as a gum; NMR (CD 81.48 (triplet. J=7 Hz) and 1.59 (singlet). overlapping (NCH CH and 1lb-CH ).4.33 (quartet. .1 7. Hz. l\&#39;-CH- ppm.  
 A 2.0 g portion of the above base is treated with isopropanolic hydrogen chloride. Recrystallization of the salt thus formed from acetonitrile gives 0.65 g title compound as the dihydrochloride salt. dec. 244246.  
 EXAMPLE ca. 170 is attained and the solution then is refluxed for 19.5 hours. The gum remaining after removal of the solvent (in vacuo) is dissolved in dilute aqueous HCl. washed with ether and reprecipitated with excess aqueous sodium hydroxide. Extraction of the precipitate with methylene chloride. washing of the organic solution with water. drying (sodium sulfate) and removal of solvent afford 24.75 g brown foam gum.  
  The preceding gum (24.55 g). 200 ml isopropanol and 100 ml ea 2.57 N isopropanolic hydrogen chloride are refluxed for 16 hours. freed of solvent and the residue is dissolved in water. After washing with ether. the acidic. aqueous solution is basified with aqueous sodium hydroxide and extracted with methylene chloride. The organic extracts are washed with water. dried (sodium sulfate) and the solvent removed. A buff solid. 21.05 g. remains which is dissolved in chloroform and flushed through a 600 g column of neutral. activity 111 alumina. Recrystallization (ethyl acetate) of the yellow solid isolated from the chloroform eluates affords 14.02 g crude product. mp. 197-199&#34;. A 400 mg por tion is recrystallized from ethyl acetate to yield 326 mg title compound. m.p. 1980-200&#34;. lv,,,,, 3.30. 5.99 pi. NMR (CDCl;,): 51.08 (triplet. J=7 Hz. CH CH;r). 4.62 (multiplet. C proton) ppm.  
  Treating 2.55 g of the above base (m.p. 197-l99) in 30 ml dry THF with 1.16 g maleic acid in ml. dry THF and diluting the resulting solution with 200 ml ether gives a salt that is twice crystallized (ethyl acetate) to afford 2.30 g of title compound. as the 1:1 mal eatc salt. dec. l43l45.  
  Although the title product may be isolated from the initial brown. foam gum. the yield is increased by treating this material with isopropanolic hydrogen chlorideisopropanol.  
 EXAMPLE vi llb-Ethyl-2.3.5.6.l1.11b-Hcxahydro-lfl- Pyridol 3..2 4.5 lPyrrolol 3.2-gllndolizine 5 To a refluxing suspension of 2.89 g lithium aluminum hydride in b 75 ml dry THF is added a solution of 9.72 g llb-ethyl-1.2.5.6.l1.11b-hexahydro-3flpyrido[3&#39;.2&#39;:4.5lpyrrolol3.2-glindol-3-one in 125 m1 dry THF. After stirring and refluxing for 2 hours. the reaction mixture is cooled and carefully decomposed with 15 ml N NaOH and filtered. The solids are thoroughly washed with warm THF and the organic fractions are freed of solvent. A residue remains which is dissolved in dilute aqueous HCl. washed with ether and S reprecipitated with excess aqueous sodium hydroxide. The precipitate is extracted with methylene chloride. washed with water and dried (sodium sulfate). Removal of solvent affords 8.73 g off-white solid. A 6.89 g portion of this material is ehromatographically purified on an 150 g column of neutral. activity 111 alumina. From the 1,19. 1/4. and H1 chloroform-benzene eluates and the early chloroform eluates are isolated 5.97 g of crude product. m.p. 20821 1. Two recrystallizations of a 3.0 g portion of the product from ethyl acetate provide 2.53 g title compound. m.p. 209-21l, 3.30 a.  
 Analysis for C, -,H .N;  
  Calculated: C. 74.65: H. 7.94; N. 17.41 Found: C. 74 *2; H. soc; N. 17.74  
  One gram of the above base (m.P. 208-21 1) in acetone is treated with excess isopropanolic hydrogen chloride and then with ether. The white solid that separates is recrystallized from methanol-acetone to provide a salt. dec. 2l8-248, which. based on elemental analyses. contains 0.5 molecule of water and 1.9 mole cule of hydrogen chloride per molecule of title base. dec. 218248)\,,,,,,&#34;&#34;&#34; 3.00. 4.08 pt.  
 EXAMPLE Vll l1b-Ethy1-2.3.5.6.1 1.1 lb-Hexahldro-l l-Methyl-lfl- Pyrido[3&#39;,2&#39;:4.5]Pyrrolo[3.2-gllndolizine Methylation of 5.31 g l1b-ethyl2.3.5.6.11.11b-hexahydro-lfl-pyridol 3.2&#39;:4.5 pyrrolol 3.2-glindolizine in 100 ml dry DMF with 1.06 g ca. 50 percent sodium hydride-mineral oil dispersion and 3.44 g methyl iodide in a manner similar to that of Example [11. Method 2. yields. from the methylene chloride extracts of the basic aqueous solution. 5.26 g crude base as a yellow oil. Chromatographic purification of the amine on an 150 g column of neutral. activity 111 alumina provides. from the benzene eluates. 4.81 g title compound as a pale pink oil. NMR (CDCl;.): 60.99 (triplet J=7 Hz. (.H CH;,). 3.85 (singlet. ll-methyl) ppm.  
  A 4.38 quantity of the above base. dissolved in 100 ml acetone. is treated with excess isopropanolic hydrogen chloride. Recrystallization (thrice) of the salt thus formed from methanol-acetone yields 2.72 g title compound as the dihydrochloride salt. dec. 263-265 (softens 250).  
 987 (1068)! and 68 ml &#34;lHF is refluxed 2 hours. concentrated to a volume of ca. 35 ml. cooled and diluted with 100 ml ether. The solid that separates is recrystallizcd front benzene to give 2.4 g -oxo-N-l2-( lflpyrrolol 2 .3-hlpy ridin-3&#39;yl )ethyl |hexanamide. m.p. 114. A,,,,, 3.04. 3.27. 5.83. (1.12 1. NMR (CDCl;,]: 62.02 (singlet. C&#39;OCH l 6.30 (multiplet. amide Nfl. exchangeable) ppm.  
  lsopropanol (450 ml). 350 g of 5-oxo-N&#39;l2-(lflpyrrolo[2.3-blpyridin-3-yl)ethyllhexanamide and 70 ml ca. 2.57 N isopropanolic hydrogen chloride are refluxed together for 4 hours. After concentration to a volume of ca. 270 ml and chilling at overnight. the light tan solid is collected and dried to provide 30.3 g crude salt. m.p. 300&#34;. A 3.5 g portion of this material is recrystallized from ethanol. thus yielding 2.8 g title compound as the hydrochloride salt. m.p. 300. )t 3.33 sh. 3.72 br. 6.12 t; NMR (d DMSO and D 0): 8180 (singlet. l2b-methyl 5.02 (multiplet. (-6 proton) ppm.  
  A 300 mg portion of the above salt in water is basi tied with excess sodium hydroxide. The base is extracted into methylene chloride. washed with water. dried (sodium sulphate) and the solvent is evaporated. A cream-colored residue remains, m.p. 2224. which is recrystallized from ethyl acetate to afford l92 mg title compound. m.p. 234237.  
 EXAMPLE 1X 1 .2,3.4.6.7.12.l2b-Octahydro-12b- Methylpyridol 3 &#39;.2&#39;:4.51Pyrrolo[ 2.3-a lQuinolizine Reduction of 5.0 g 1.2.3.4.6.7.12.l2b-octahydro- 12b-methylpyridol 3&#39;.2:4.5 ]pyrrolo[ 2.3-a lquinolizin- 4( lfl)-one with 2.0 g lithium aluminum hydride and 350 ml dry THF in a manner similar to that of Example 1!. Method 2 yields. from the tetrahydrofuran fraction, 5.1 g 108% of crude product. m.p. 156-l58. A 4.5 g portion of the product in methanol-ether is treated with excess isopropanolic hydrogen chloride. Successive recrystallizations of the salt from isopropanol. methanol-ether. nitromethane and methanol-acetone (twice) produce 1.2 g title compound as the dihydrochloride salt. bearing 0.5 molecule of water per molecule of dihydrochloride. melting range 215245.  
  A 400 mg portion of crude product (m.p. 156158) is recrystallized from ether to give 025 g title com pound. m.p. 16ll(:\2. A,,,,,,&#34; 3.25 br ;1..  
 l23.4.6.7.12.1Zb-Octahydro-12.12b-Dimethylpyrido l3&#39;.2&#39;:4.5]Pyrrolo[2.3-aIQuinolizine Methylation of 2.4 g 2.3.6.7.]2.l2b-hexahydro-12b methylpyrido[3&#39;.2&#39;:4.5]pyrrolo[2.3-a]quinolizine (2.4 g) in 40 ml dry DMF with 0.53 g ca. 50 percent sodium hydridemineral oil dispersion and 1.44 g methyl iodide is conducted in a manner similar to that of Example lll. Method 2. Benzene extracts of the basic aqueous frac tion are washed with brine and placed on a 75 g column of neutral. activity lll alumina. Elution of the chromatography column with 3/25 ether-benzene products 2.0 g of the title compound as an oil. NMR (CDCl;.): 51.49 (singlet. l2b-methy1). 3.96 (12-methy1) ppm.  
  A l9 g portion of the above base is treated with excess isopropanolic hydrogen chloride. Recrystallization of the salt thus formed from ethanol yields 1.7 g title compound as the dihydrochloride salt. dec. 288.  
 EXAMPLE Xl 2.3.6.7.] 2.12b-Hexahydro-l2.12b- Dimethylpyridol 3 .2 &#39;:4.5 lPyrrolol 2.3a lQuinolizin- 4( lib-One A solution of 2.5 g 2.3.6.7.12.12b-hexahydrod2bmethylpyridol 3 &#39;.2 14.5 ]pyrrolo[ 2.3-a lquinolizin- 4( lfll-one in 36 ml dry DMF is methylated with 0.53 g ca. 50% sodium hydridemincral oil dispersion and 1.42 g methyl iodide in a manner similar to that of Example lll. Method 1. Benzene extracts of the basic aqueous fraction. after washing with brine and drying (magnesium sulfate) are placed on a g column of neutral. activity 111 alumina. Development of the column with 3/20 ether-benzene yields 1.2 g title com pound. m.p. 159l60.  
  Treating 1.1 g of the above base in ether with excess isopropanolic hydrogen chloride produces a salt which is recrystallized from nitromethane to afford 0.7 g of the title compound as the dihydrochloride salt. m.p. 253. i\,,,,,,&#39; 4.54. 6.11, sh. 6.15 a; NMR (dDMSO):  
  1.78 (singlet. 12b-methy1), 4.11 (singlet. l2-methyl). 4.95 (multiplet. C-o proton) ppm.  
 EXAMPLE Xll 1.2.3.o.7.Sa-Hexahydro-8a-Methylindolizin- 8(5fl)-One Toluene (200 ml). 4.1 g sodium methoxide and 16.0 g 2-carboxy-2-methyl-1-pyrrolidinebutyric acid, dimethyl ester are refluxed 3 hours. The mixture is extracted with water and the combined aqueous extracts are adjusted to pH with concentrated HCl. After heating three hours at ca. the acidic solution gives a positive ferric chloride test. Therefore heating is continued 10 hours longer whereupon a negative test is attained. The solution is evaporated to a small volume and treated with excess sodium carbonate and ether. The ether phase is separated and the residue is triturated thoroughly with ether. After drying (MgSO the combined ethereal fractions are freed of solvent and the residual oil is distilled to afford 4.4 g l.2.3.6.7.8ahexahydro-8a-methy1indo]izin-8(51-1 )-one. b.p. -l l9l22 mm; A,,,,,,&#34; 5.83 NMR (C 3) 50.95 (singlet. 8a-methyl) ppm.  
 EXAMPLE Xlll 2.3.5.6.l1.11b-Hexahydrollb-Methyl-ll-Propyl-lfl- Pyrido[ 3&#39;,2&#39;:4.5]Pyrrolo[3.2-g]lndolizine Propylation of 6.82 g 2,356.1 1.1 lb-hexahydro-l lbmethyll fl-pyridol 3 &#39;.2&#39;:4.5 lpyrrolol 3.2-g1indo1izine in ca. 100 ml dry DMF with 1.59 g 50 percent sodium hydride-mineral oil dispersion and 7.07 g n-propyl ptoluenesulfonatc in a manner similar to that of Example Ill. Method 2. yields. from the benzene extracts of the basic. aqueous fraction. 72 g crude product. Purifi cation of the product on a 240 g column of alumina (neutral. activity 111) affords. from the benzene eluates. 4.99 g title compound as a pale-pink gum. NMR (CDCl 51.03 (triplet, i=7 hz. Hz. 2CH3)- 1.58 (singlet. llb-methyl) and 4.17 (multiplet. 1 lmethylene) ppm.  
  The above base. in acetone. is treated with excess isopropanolic hydrogen chloride. Two recrystallizations of the salt thus formed from methanol-acetone give 2.99 g title compound as the dihydrochloride salt. solvated \i ith one-third molecule of water per molecule of dihydroeliloride. dcc. 232.5-239.5 (softens 227). M? 2. 15. 4.2:. 4.40. 6.15 ,1.  
 EXAMPLE Xl\&#39; ll-Allyl-2.3.5.6.l l.l llvHcxahydro-l lb-.&#39;\lethyl lfi- 5 Pyridol 3 &#39;.2&#39;:-1.5 1 Pyrrolol 3.2g llndolizinc Allylation ol- 2.3.5.6.] l.l lbhexahydro-l lb-methyllfl-pyridol3&#39;.2&#39;:4.5 lpyrrolol3.2-glindolizine (5.69 g) in ca. 100 ml dry DMF with 1.32 g ca. 511 percent sodium hydride-mineral oil dispersion and 3.33 g allyl bromide in a manner similar to that of Example 111. Method 2. affords. from the methylene chloride e\- tracts of the basic. aqueous fraction. 5.61 g gummy amine which are purified on a 200 g column ofalumina (neutral. activity 111 alumina). From the 1:1 benzenehexane and benzene eluates are isolated 3.03 g title compound as a gum. )v,,,,, 6.13. 10.94 1; NMR (CDCl;,): 81.55 (singlet. 11b-methyl).4.95 (multiplet. ll-NCH-; and CH=CH. protons) and 6.05 (multiplet. lll\lCH CH=CH proton) ppm.  
  Treating 2.83 g ofthe above base in acetone with ex; cess isopropanolic hydrogen chloride yields a salt which is recrystallized (twice) from ethanol to provide 212 g title compound as the dihydrochloride salt. solvated with one-third molecule of u ater per molecule of dihydrochloride. dec. 239-2-12 (softens 230). A,,,,,,&#34; 2.93. 6.11. 10.60 p.  
 EXAMPLE X\&#39; ll.l1b-Diethyl2.3.5.6.l1.1lbHexahydro-lfl-Pyrido [3&#39;.2&#39;:4.5 IPyrroloI 3.2- Ilndolizine Ethylation of ll-ethyl-2.3.5.6.1 1.1 lb-hexahydrolfl-pyridol3&#39;.2&#39;:4.5]pyrrolo|3.2-g1indolizine (5.31 g) in Cat. 110 ml dry DMF with 1.16 g Ctt. sodium hydride mincral oil dispersion and 3.77 g ethyl iodide according to the general procedure of Example 111. Method 2. yields. from the benzene extracts of the basic. aqueous fraction. a yellow oil. which is purifed on a 200 g column of neutral. activity 111 alumina. From the benzene eluates are isolated 4.81 g of crude prod act as a pale-pink solid. mp 80.0-83.5.  
  A 500 mg portion of the crude product is distilled at 92&#34;99/l0 mm. to yield 438 mg white title compound. m.p. 80.583.0. NMR (CDCl;;): 80.98 (triplet. J=7 Hz. 11bCH Ch,- 1.45 (triplet. 1 Hz. -NCH:Ch protons) and 4.28 (multiplet. llmethylene) ppm.  
  Treating 4.17 g of the crude product (mp. 50 8().()-83.5) in acetone with excess isopropanolic hydrogen chloride and recrystallization (twice) of the resulting salt from methanolacetone afford 3.43 g of the title compound as the dihydrochloride. dcc. 237242 (softens 215).  
 EXAMPLE XVI l1b Ethyl-l.2.5.6.11.1lb-Hexahydro-ll-Methyl-3fl- Pyridol 3&#39;,2:4.5 |Pyrrolo[ 3.2-gllndolizin-3-One In a manner similar to that described in Example 11!. Method 1. llb-ethyll.2.5.6.l 1.1 lb-hexahydro-3flpyridol3&#39;,2&#39;:4.5]pyrrolo[3.2-g]indolizin-3-one (2.04 g) in ca. 60 ml dry DMF is methylated with 0.42 g 50 percent sodium hydride-mineral oil dispersion and 1.25 h g methyl iodide. Benzene extracts of the basic. aqueous fraction are washed with water and with brine and dried [sodium sulfate). Removal of the solvent affords 2.00 g yellow gum which is purified on a g. alumina (neutral. activity 111) column. From the l/4 and 1f] chloroformbenzene eluates are isolated 1.77 g title compound as a colorless glass. A,,,,, 5.96;; NMR (CDCl 80.98 (triplet. J=7 Hz. 1lbCH. CH;;). 3.83 (singlet. ll-methyl) and 4.50 (multiplet. (-5 proton) Treating the above base (1.63 g) with e\eess isopropanolic hydrogen chloride gives a salt which. unpon recrystallization (twice) from methylene chloride acetone provides 1.18 g title compound as the hydro chloride salt. dec. 264267.  
 EXAMPLE XVll l 2-Ethyl-1 .2.3.6.7.12.l 2b-Octahydro-l 2b- Methylpyridol 3&#39;.2&#39;:4.5 ]Pyrrolo[ 2.3-a lQuinolizine Ethylation of 2.41 g l.2.3.4.6,7.12.l2b-octahydrol2b methylpyrido[ 3.2:4.5 lpyrrolol 2.3-a lquinolizine in 36 ml dry DMF with 0.53 g sodium hydridemineral oil dispersion and 1.7 g ethyl iodide in 5 ml dry DMF is conducted in a manner similar to that of Example 11]. Method 2. Benzene extracts of the basic. aqueous fraction are washed with brine and placed on an g column of neutral. activity 111 alumina. From the benzene eluates are isolated 1.6 g of the title compound. mp. 80. NMR (CDCl;.): 81.45 (singlet. l2b-mcthyl) and 430 (multiplet. 12 NCh. ppm.  
  An 1.5 g quantity of the above base is converted to the hydrochloride salt which. after recrystallization from acetonitrile. affords 0.8 g title compound as the dihydrochloride salt. m.p. 232234.  
 EXAMPLE XVlll 12-A1l vl-l.2.3.4.6.7.l2.12b-Octahydro-12b- Methylpyridol 3 &#39;.2&#39;:4.5 ]Pyrrolo[ 2.3-a lQuinolizine Employing a procedure similar to that of Example 111. Method 2. 4.5 g l.2.3.4.6.7.12.12b-octahydro-l2- methylpyrido [3&#39;.2&#39;:4.5]pyrrolol2.3-a1quinolizine in 70 ml dry DMF are reacted with 0.98 g 50 percent sodium hydride-mineral oil dispersion and 2.37 g allyl bromide in 5 ml dry DM F. Chloroform extracts of the basic. aqueous solution are washed with brine. dried (magnesium sulfate) and freed of solvent. Chromatographic purification ofthe residue on an g column of neutral. activity 111 alumina yields. from the benzene eluates. 2.9 g title compound as a gum. NMR (CDClalI 81.48 (singlet. lZb-methyl). 5.02 (multiplet. N-CH and CH=CH 6.02 (multiplet. N-CH ,CH=CH2) PPm.  
  conversion of 2.8 g of the above base to the hydrochloride salt and recrystallization of the crude salt from methanolacetone give 1.6 g of the title compound as the dihydrochloride salt. solvated with one-fourth molecule of water per molecule of salt. mp. 233-5 )t,,,, 2.95. 6.13. 10.65 1.  
 EXAMPLE XIX 1.2.5.6.11.1lb-Hexahydr0-1lb-Propyl-Bfl- Pyriclo[3.2:4.5]Pyrrolo[3.2-gllndolizin-3-One Butyl cellosolve (500 ml). 40.32 g 7-azatryptamine and 38.92 g 4 oxoheptanoic acid {See US. Pat. No.  
 3 2.577.133. (CA. 46. til-17h). also T. M. Patrick. J.  
 Org. Chem 17. 1009 (1952)]are reacted and purified in the manner described in Example V. The product (47.9 g) isolated after chromatography is recrystallized from ethyl acetate to yield 37.55 g title compound.  
  l9 m.p. 180.5-l84.0. A,,.,, 3.12. 5.9811. NMR (CDCh): 50.90 (triplet. J=6 H2. 11b(&#39;H- .CH;,). 4.55 (multip1et.C-5 proton) ppm.  
  A solution of 4.04 g of the above base in dry THF is added to 1.74 g fumaric acid in the same solvent. Subsequent addition of ether and chilling afford a salt which is recrystallized from ethyl acetate (twice) and from ethyl acetateether to provide 1.11 g title compound as the mono fumarate salt. dec. 164.5-l67.0.  
  EXAMPLE XX 1.2.5.6.11.1lb-Hexahydro-ll-Methyl-llb-Propyl-3fl- Pyrido[ 3&#39;,2&#39;:-1.5IPyrrolol3.2-gIlndolizin-3&#39;One In a manner similar to that given in Example 111. Method 1. 1.2.5.6.11.1lb-hexahydrollb-propyl3flpyrido[3&#39;.2&#39;:4.5lpyrrolol3.2-glindolizin-[S-one (8.08 g) in 75 ml dry DMF is methylated with 1.58 g 50 percent sodium hydride-mineral oil dispersion and 4.68 g methyl iodide. Benzene extracts of the basic aqueous solution are washed with water and with brine and dried (sodium sulfate). The gum (8.5 g) remaining after removal of the solvent is chromatographically purified on 250 g neutral. activity 111 alumina. From the [/4 chloroform-benzene and 1/1 chloroformbenzenc eluates are isolated 6.70 g title compound as a colorless gum. )t,,.,.,&#34;&#34;&#34;&#39;3 6.02;.t&#39;. NMR (CDCl;.): 50.94 (triplet. i=6 Hz. llb-CH C&#39;H; 3.85 (singlet. ll-methyl). 4.53 (multiplet. (-5 proton) ppm.  
  Treating the above base (6.6 g) in ether with excess isopropanolic hydrogen chloride gives a salt which. upon recrystallization (twice) from methylene chloride-acetone affords 5.1 l g title compound as the hydrochloridc salt. dec. 2536 (softens 220).  
 EXAMPLE XXI 2.3.5.6.11.1lb-Hexahydro-llb-Propyl-lfl- Pyrido[3&#39;.2&#39;:4.5 ]Pyrrolol 3.2g1lndolizine Reduction of2 l .55 g 1.2.5.6.1 1.1 lb-hexahydro-l 1bpropyl-3H;pyrido[ 3 &#39;,2 24.5 lpyrrolo[ 3 .2-g lindolizin- 3-one with 6.07 g lithium aluminum hydride and 250 ml dry THF is conducted in a manner similar to that of Example V1. The solid isolated from the methylene chloride extracts is recrystallized from ethyl acetate. thereby affording 16.8 g title compound. m.p. 146.0-l48.5.NMR(CDC11t):80.87 (triplet. J=6 Hz. llb-CH CH;.) ppm.  
  A 5.11 g portion of the above base in dry THF is treated with excess isopropanolic hydrogen chloride and then with acetone. Two recrystallizations of the salt thus formed from methanol-acetone yield the title compound as the dihydrochloride. 1.35 g dec. 25126l (softens 235).  
 EXAMPLE XXll 2.3.5.6.1 1.1 lb-Hcxahydro-ll -Methyl-l lb-Propyl l fl-Pyridol 3&#39;,2&#39;:4.5 ]Pyrrolo[ 3.2- g]lndolizine Methylation of 2.3.5.6.1 1.1 lb-hexahydrol lbpropyll l -l -pyrido{ 3 &#39;.2:4.5 ]pyrrolo( 3.2-glindolizine (7.66 g) in 125 ml dry DMF with 1.59 g 50 percent sodium hydridc mineral oil dispersion and 4.68 g methyl iodide is carried out in a manner similar to that of Example 111. Method 2. From the benzene extracts of the basic. aqueous fraction are isolated 6.65 g brown gum which is placed on a 350 g column of neutral. activity Ill alumina. Removal of solvent from the 1:1 benzenechloroform eluates gives 6.22 g crude title compound as a colorless gum. NMR ((DCLJ: 50.87 (triplet.  
 .l=6Hz. 1 1bCH CH:,). 3.80 (singlet. ll-methyl ppm.  
  A 6.12 g portion of the above base in ether is treated with excess isopropanolic hydrogen chloride. Recrystallization (thrice) of the salt thus formed from methanol-acetone and from methanol-ether (small amount of HCl gas added to insure complete salt formation) provides 2.28 g title compound as the dihydrochloride. dec. 247252 (softens 238).  
 EXAMPLE XXlll l 1-Buty|-2.3.5.6.11.1 lb-Hexahydro-l lb-Methyl-lfl- Pyrido[3&#39;,2:4.5lPyrrolol3.2-gl1ndolizine Butylation of 5.69 g 2.3.5.6.1 1.1 lb-hexahydro-l 1brnethyllfl-pyridol 3 &#39;,2 24.5 ]pyrro1o[ 3 .2-glindolizine in ml dry DMF with 1.32 ea. 50 percent sodium hydride-mineral oil dispersion and 3.75 g l-bromobutane is conducted in a manner similar to that of Example 111, Method 2. From the benzene extracts of the basic. aqueous fraction are isolated a crude base (6.37 g) which is purified on a 200 g column of neutral. activity 111 alumina. The gum (3.41 g) eluted with benzene is distilled to provide 2.71 g title compound as a viscous. pale yellow oil. b.p. 137-8/10 mm; NMR (CDCIg): 61.00 (triplet. J=7 Hz. CH CH 1.58 (singlet 11bmethyl). 4.24 (multiplet. ll-methylene) ppm.  
  A 21.4 g portion of the above base in acetone is treated with isopropanolic hydrogen chloride followed by addition of ether. Recrystallization (twice) of the salt thus formed from methylene chloride-acetone yields 0.63 g title compound as the dihydrochloride salt. dec. 235239 (softens 210).  
 EXAMPLE XXIV 2.3.5.6.1 1.1 lb-Hexahydro-l l-lsoamyl-l lb-Methyllfl-Pyrido[3&#39;.2&#39;:4.5 ]Pyrrolo[3.2-gllndolizine 1.59 (singlet. 4.23 (multiplet. l1- methylenc) ppm.  
  Dissolution of 3.77 g of the above base in acetone. addition of excess isopropanolic hydrogen chloride and subsequent treatment with ether provide a salt that is twice recrystallized from 2 propanol. A final recrystallization from acetonitrile gives 284 title compound as the dihydrochloride salt solvated with two-thirds of a molecule of water per molecule of dihydrochloride. dec. l97-206 (softens 182) )t,,.,, 2.93. 401p.  
 EXAMPLE XXV l --2.3.5.6.l 1.1 lb-Hexahydro-l 1.1 lb-Dimethyllfl- Pyridol 3&#39;.2&#39;:4.5 lPyrrolol 3.2-gllndolizine Dihydrochloride A solution of 56.45 g 2.3-dibenzoy1-2(R):3(R)- tartaric acid monohydrate ([01],, l 14.7 (c. 1.028. methanol)] in 200 ml warm (50) methanol is treated 1 lb-methyl with a solution of 36.20 g 2.3.5.6.1l.llb-hexahydrol 1.1 lb-dimethyl-lfl-pyridol 3&#39;.2&#39;:-l.5 ]p \rrolo[ 3.2- glindolizine in 100 ml warm (50) methanol. After seeding. the mixture is kept at ca. 25 for 4 hours and at +8 for 16 hours. The product is collected and thrice recrystallized from methanol to afford 29.59 g (66%) l-2.3.5.6.1l.1lb-hexahydro-ll.llb-dimethyl-IH- pyridol3&#39;.2&#39;:4.5]pyrrolo[3.2-glindolizine. 2.3-dibenzoyl-2(R):3(R)-tartrate (1:1). dec. l75.5l77.0. [01],, -97.5(c. 1.006. DMF).  
  The preceding salt. 27.77 g. suspended in 0.25 l. water. is treated with ml 50 percent w/w aqueous sodium hydroxide and the amine is extracted into ether. After successive washings with water and with brine. the ethereal solution is dried over sodium sulfate. Re moval of solvent gives 10.67 g (95 l- -2.3.5.6.1 l.llb-hexahydro-ll.1lb-dimethcyl-lflpyridol3&#39;,2&#39;:4.5]pyrrolo[ 3.2-glindolizine as a barely yellow. viscous oil. [a ].F-l l5.8(c. 0.9605. methanol).  
  Dissolution of 10.42 g of the preceding oily base in 150 ml absolute ethanol and addition of 40 ml 2.48 N isopropanolic hydrogen chloride provide a salt. Two recrystallizations of the salt from methanol-acetone yield 11.26 g (8371) title compound is a white solid. dec. 3065-80. [al -826 (c. 097]. methanol).  
 EXAMPLE XXVl d-2.3.5.6.1l.llb-Hexahydro-ll.llb-Dimethyl-lfl- Pyrido[3&#39;.2:4.5lPyrrolol3.2-gllndolizine. Dihydro chloride The residue isolated upon evaporation of the methanolic mother liquor from which the crude levo isomer separated (see Example XXV) is shaken with 400 ml water 20 ml 50 percent w/w aqueous sodium hydroxide and the liberated base is extracted into ether. After washing with water. with brine and drying (sodium sulfate). the solvent is evaporated to provide a brown oil. To remove any non-basic contaminants. the oil is dissolved in 200 ml N hydrochloric acid and the acidic so lution is washed thoroughly with ether. Basification of the acidic solution is followed by extraction with ether and the ethereal extract is washed with water and with brine. After drying ofthe ethereal solution (sodium sulfate) and removal of solvent 17.84 g crude dextro base are isolated as a brown gum. (01],, +89.00 (c. 1.002. methanol).  
  This gum (17.64 g) in ml methanol is added to a warm (-50) solution of 27.51 g 2.3-dibenzoyl- 2(S]:3(S)-tartaric acid monohydrate ([01],, 115.7  
 AJI  
 (c. 1.025. methanol)]in 90 ml methanol. After seeding.  
 the mixture is kept at +5 for 22 hours. The salt is collected and thrice recrystallized from methanol. with washing with small amounts of cold methanol and with ether after each recrystallization. to produce 27.06 g lfl-pyridol 3 &#39;.2&#39;4.5 lpyrrolol 3.2-glindolizine. 2.3- dibenzoyl-2(S):3(S)-tartrate (1:1). dec. l767. [01],, +990 (c. 1.026. DMF).  
  Conversion of 25.34 g of preceding salt to the free base is carried out in the same manner as for the levo isomer (see Example XXV) thereby yielding 9.74 g (96%) d-2.3.5.6.l l.l lb-hexahydro-l l.l lb-dimethyllfl-pyrido[3&#39;.2:4.5]pyrrolo[3.2-glindolizine as a barely yellow. viscous oil. [al +1155 (c. 0.992. methanol).  
  A 9.49 g portion of the preceding base in 150 ml absolute ethanol is treated with 35 ml 2.48 N isopropanolic hydrogen chloride. The solid that forms is collected and twice recrystallized from methanold-2.3.5.6.l l.l lb-hexahydro-l l .l lb-dimethylacetone to afford 9.97 g (81%) title compound. dec. 306.58.0. lul +82.4 (c. 1.061. methanol).  
 EXAMPLE XXVll 2.3.5.6.] l.l lb-Hexahydro-l l.l lb-Dimethyl-lfl- Pyrido[3&#39;,2:4.5]Pyrrolo[3.2-gllndolizine a. Methylhydrazine (36.8 g) and 12.8 g 2- bromopyridine are refluxed 6 hours and then the reaction solution is concentrated at 50 (in vacuo] to an oil. is mixed with ice and is basified with 50 percent w/w aqueous sodium hydroxide. The mixture is extracted with ether and the extract is washed with brine and dried (MgSO Removal of the ether and distillation of the residue gives 49 g N-methyl-N-(2-pyridyl) hydrazine. b.p. 74-6/3mm. NMR (CDCL 83.25 (singlet. N&#39;CHH) G. Palazzo and L. Baiocchi lAim. (him. (Rome). 55. 935 (1965): CA. 63. l6335h (1965] prepared N-methyl-N-(2-pyridyl) hydrazine by methylation of Z-hydrazinopyridine with methyl iodide and sodium hydride. b.p. 68/0.5 mm.  
  b. Employing a reflux period of 5 hours. 146.1 g 2- carboxy-Z-methyLl-pyrrolidinebutyric acid. dimethyl ester and 35.7 g sodium methoxide in 1.5 liter toluene are reacted as in Example Xll. After heating at ca 100&#34; for 20 hours. the acidic aqueous solution is evaporated to dryness and the residue is extracted into methanol and is filtered to remove insolubles. The extract is freed of solvent and the residue is redissolved in methanol (200 ml). filtered. and the filtrate is concentrated to a volume ofca. ml. Addition of 450 ml acetone and chilling (+5&#34;) afford. after thorough drying. 58.2 g 1.2.- 3.6.7.8a-hexahydro-8a-methylindolizin-8(5H )-one. hydrochloride. m.p. 19920l. 4.02. 5.77 41.; NMR (D..DMSO): 81.64 (singlet. Zia-methyl) ppm.  
  c. Absolute ethanol (25 ml). 2.5 g N-methyl-N-(2- pyridyl)hydra7.ine (see part a above) and 3.8 g 1.2.3.6.- 7.tla hexahydro-tia-methylindolizin 8( 5fl)-one. hydrochloride are heated on the steam bath and the solution is adjusted to pH ca. 1 with isopropanolic hydrogen chloride. The solution then is heated on the steam bath with evaporation of the solvent and the gummy residue is dissolved in water. Basification of the aqueous solution with aqueous sodium hydroxide is followed by extraction of the mixture with chloroform. The organic extracts are washed with brine. dried (MgSOfl and freed of solvent to give a liquid residue. Distillation of this material at l58/3.l0mm affords 3.8 g 1.23.6.7.- 8a-methylindo1izin-8(Sfl)-one. N-methyl-N-( 2- pyridyHhydrazone. NMR (CDCl;,): 51.33 (singlet. C-Me). 3.17 (singlet. N-methyl) ppm.  
  (1. A solution of 0.5 g 1.2.3.6.7.8a hexahydro-8amethylindolizin-8( 5H) one. N-methyl-N-pyridyl hydrazone in 7 ml diphenyl ether is refluxed for 30 minutes. cooled. diluted with ether and extracted weith dilute aqueous hydrochloric acid. The acidic extract. after washing with ether. is basified with concentrated so dium hydroxide and the mixture is extracted with chlo roform. After washing with brine and drying (MgSO the chloroform solution is freed of solvent and the resi due is chromatographed on 12 g of neutral. activity 111 alumina. From the benzene eluates are isolated 108 mg gum which is dissolved in ether and treated with excess isopropanolic hydrogen chloride. The solid thus formed is triturated with ether and thoroughly dried to provide 85 mg of salt. dec. 30l-4 (softens 298) which is shown to be the title compound. dihydrochloride. by melting point. mixture melting point. thin-layer chromatographic [R and NMR spectral comparisons with the product of Example lll. Method l.  
 EXAMPLE XX\&#39;lll 1.2.5.61l.lllvHesahydro-llbMethyl-3fl- Pyridol 3 &#39;.2:4.5 lPyrrolol 3.2-g]lndolizin&#39;3-Thione Dimethoxyethanc 100 ml). 2.4 g phosphorus penta sulfide and 2.4 g l.3.5.(1.ll.llb-hexahydro-llb methylJfl-pyrido l3&#39;.Z&#39;:4.5lpyrrolo[3.2-glindolizin- 3-one are refluxed 4 hours. After cooling. dilute aque ous ammonium hydroxide is added. the reaction mixture is extracted with chloroform and the organic solution is washed with dilute aqueous ammonium hydroxide. with brine and is dried (MgSOQ. Passage of the dried chloroform solution through a 100 g Column of neutral. activity lll alumina and further elution with chloroform provide 23 g solid. Recrystallization of this material from methylene chlorideacetone yields 0.8 g title compound. dec. 299300.  
 Analysis for C H NS Calculated. C. 65.34. H. 5.83. N. H 33. S. I140 Found: C. (14.93. H. 6.09. N. 10.05. S. llfil Exposure of 0.7 g of the above product in methylene chloride to excess isopropanolic hydrogen chloride. followed by the addition of ether yields a salt which is recrystallized from methanol-acetone to give 06 g title compound as the hydrochloride. dec. 300( softens 285).  
 EXAMPLE XXlX Refluxing of l.] g 1.2.5.6.] l.l lb-hexahydro-l lbmethyl-3l -l -pyrido[3&#39;.2&#39;:4.5)pyrrolo[3.2-g1indolizin-3- thione in 150 ml absolute ethanol with [5 g (wet weight) of Raney nickel (Grace Co. No. 28. previously slurried with distilled water and then with absolute ethanol) for 2 hours is followed by filtration and washing of the insolubles with [00 ml hot ethanol. The ethanolic filtrates and washings are combined. freed of solvent and the residue is dissolved in dilute aqueous hydrochloric acid and is washed with ether. Basification of the acidic solution is followed by ether extraction and the extracts are washed with brine and dried (magnesium sulfate). Evaporation of the solvent yields 06 g solid which is flushed through a g column of neutral. activity lll alumina with chloroform. The solid iso lated from the chloroform eluates is recrystallized from methylene chloride-ether to provide 0.2 g solid. m.p. 290. Removal of solvent from methylene chlorideether crystallization mother liquor gives a solid that is recrystallized (twice) from ether. The compound thus isolated. m.p. 196.5 is shown to be the title amine by melting point. mixture melting point. and IR and NMR spectral comparisons with the product of Example ll. Method 1.  
  The conditions employed in Examples XXVIll and XXIX are similar to those described in Us. Pat. No. 3.454.583.  
  EXAMPLE XXX 2.3.5.6.] l.l lb-Hexahydro-l l.l lb-Dimethyl-lfl- Pyrido [3&#39;.Z:-l.5lPyrrolol3.2-gllndolizine 4-Oxide A solution of 2.4l g 2.3.5.6.l l.l lb-hexahydroll.] tb-dimethyl-lfi-pyridol3&#39;.2:4.5]pyrrolo[3,2- glindolizine in 25 ml dry THF is stirred and kept at 0 while a solution of 1.73 g m-chloroperoxybenzoic acid in 25 ml dry THF is added dropwise (5 minutes). After stirring 0.5 hour at 0, 9.0 ml of 2.3 N isopropanolic hydrogen chloride are added and stirring is continued for 0.5 hour. Acetone (20 ml) is added to aid filerability and the product is collected. washed with acetone and dried. Two recrystallizations ofthe crude product from methanol provide l.7. g title compound. as the dihyd rochloride. dec. 270-278.  
 EXAMPLE XXXl In a manner similar to that of Example lIl. Method 2. 6.82 g 2.3.5.6.1l.llb-hexahydro-llh-methyflL-lpyridol 3&#39;.2&#39;: 4.5]pyrrolol3.2-glindolizine in I00 ml dry DMF is reacted with 1.59 g 50 percent sodium hydride mineral oil dispersion and 4.99 g bromopentane. From the benzene extracts of the basic. aqueous solution are isolated 8.2 g viscous yellow gum which is chromatographically purified on a 250 g column of neutral. activity lll alumina. Removal ofsolvent from the benzene eluates provides 4.75 g title compound. as a pale pink gum. NMR (CDClnll 50.93 (triplet. J=6Hz. CH CH;;). L63 (singlet, l lb methyl). 4.25 (multiplet. ll-methylene) ppm.  
  Treating a solution of 4.60 g ofthe above base in acetone with excess isopropanolic hydrogen chloride followed by the addition of ether gives a salt which is recrystallized (twice) from methylene chloride-acetone to yield 3.04 g title compound as the dihydrochloride salt solvated with one&#39;fourth ofa molecule of water per molecule of dihydrochloride. dec. l94-l99, AWUM 2.92. 4.l5p..  
 EXAMPLE XXXll Alkylation of 6.82 g. 2.3.5.6.22,22b-hexahydro-l lbmethyl-lH-pyridoB&#39;,2&#39;:4.5]pyrrolo[3,2g]indolizine in lOO ml dry DMF with L59 g. ca. 5071 sodium hydride-mineral oil dispersion and 5.45 g. n-hexyl bromide in a manner similar to that of Example lll. Method 2. affords. from benzene extracts of the basic. aqueous fraction. 8.32 g. yellow gun. This material is dissolved in 100 ml. chloroform. adsorbed onto g. neutral. activity lll alumina. freed ofsolvent and placed on a 500 g. dry column of the same type of alumina. The column is developed with chloroform. The most mobile. major band (visualized with u.v. light) is removed and is thoroughly extracted with 1:3 methanolchloroform. Evaporation of the solvent from the extracts provides 4.11 g. title compound as a gum. NMR (CDCl;;): 8 0.90 (triplet. J=7Hz. CH CH 1.63 (singlet. llb-methyl). 4.23 (multiplet. l l-methylene) ppm.  
  Dissolution of 3.86 g. of the above base in ether and addition of excess isopropanolic hydrogen chloride gives a salt which is recrystallized (twice) from acetone to provide l.3l g. title compound as the dihydrochloride salt. dec. l88l94.  
 EXAMPLE XXXlll l1-Hepty1-2.3.5.6.1 1.1 lb Hexahydro-l lb-Methyl-1fl Pyrido[ 3 &#39;.2&#39;:4.5 lPyrrolol 3.2-g llndolizine In a manner similar to that of Example 111. Method 2. 3.41 g. 2.3.5.6.l1.1lb-hexahydro-l1b-methyl-lflpyrido[3&#39;,2:4.5l-pyrrolol 3.2-g]indolizine in 55 ml. dry DMF is reacted with 0.79 g. ca. 50% sodium hydridemineral oil dispersion and 2.96 g. lbromoheptane. From the benzene extracts ofthe basic. aqueous fraction are isolated 4.35 g. crude base which is chromatographically purified on an 130 g. column of neutral. activity 111 alumina. Removal of solvent from the benzene eluates provides 2.04 g. title compound as an oil. NMR (dDMSO). 5 (1.90 (triplet. J=7H2. CH- ,CH; 1.60 (singlet. llb-methyl). 4.23 (multiplet. ll-methylene) ppm.  
  Addition of an ethereal solution of the above base (1.90 g.) to an ethereal solution of 0.68 g. maleic acid yields a salt which is recrystallized (twice) from methylene chloridetliisopropyl ether to give 1.45 g. titte compound as the (mono) maleate salt. dec. 8488.  
 EXAMPLE XXXlV dextro-2.3.5.6.l1.11b-Hcxahydro-l l.llb-Dimethyllli-Pyrido[3&#39;.2:4.5]Pyrrolo[3.2gllndolizine 4-Oxide Dihydrochloride To 6.03 g. dextro-2.3.5.6.l1.11b-hexahydro-l1.1lbdimethyl-lfl-pyridol3.2&#39;:4.5lpyrrolol3.2-g1indolizine (see Example XXVI) in 100 ml. dry THF is added. during 5 minutes. a solution of 4.53 g. mchloroperoxybenzoic acid in 50 m1. dry THF keeping the reaction mixture at to C. After stirring minutes. 22.2 ml. ca. 2.48N isopropanolic hydrogen chloride are added and a thick mixture is formed. Acetone 150 ml.) is added and. after stirring 20 min. at 0, the solids are collected. washed with cold acetone and dried. The product is recrystallized from methanolacetone (twice). with washing of the salt with cold acctone after each recrystallization. to provide 4.54 g. (60% title compound. dec. 27l-288 (varies greatly with the rate of heating); 1011 66.8(c. 1.0024. MeOH) (determined in a l dm. cell using a Perkin- Elmer Model 141B automatic polarimeter).  
  Although this N-oxide is optically stable in water or methanol. either as the protonated or non-protonated species. racemization occurs in the solution formed by adding excess triethylamine to a suspension of the N- oxide dihydrochloride in chloroform. Similarly. the N- oxide dihydrochloride is optically stable in dimethylsulfoxide solution but is rapidly racemized upon the addition of excess triethylamine.  
 EXAMPLE XXXV 2.3.5.6.1 1.1 1b-Hexahydro-2.l lb-Dimethyhlfl- Pyridol3&#39;.2&#39;:4.5 ]Pyrrolol 3.2g|lndolizine Butyl cellosolve (1 liter). 64.48 g. 7-azatryptaminc and 52.04 g. Z-methyllevulinic acid [R. J. Reynolds Tobacco Co.. C.A.. 67. No. 32333k. 3041 (1962); V. B. Piskov. C.A.. 62. l3037b (1965)] are heated to reflux with removal of the volatiles (250 ml. distillate collected) and the solution is refluxed for 17 hr. Solvent is removed in vacuo and the residue is triturated with ethyl acetate and with ether and dried to give 553 g. solid. mp. 2l2-9 (softens 195). The ethyl acetate and ether triturates are freed of solvent and the residue (43 g.) is chromatographically purified on an 1.3 kg. column of neutral. activity 111 alumina. From the ch11 roform eluates are isolated 14.1 g. solid. mp. 2l36 (softens 210) which. with the main product. is recrystallized from ethyl acetate thereby providing 1.2.5.6.]- l.1 lb-hexahydro-2.l lb&#39;dimethyl-3flpyrido[3.2&#39;:4.5]pyrrolo[3.2 -g]indolizin-3-one. mp. 22 l.5-4 (softens 217). MWJ 3.14. 6.00 NMR (CD0 6 1.23 (doublet. J=6Hz. Z-methyl). 1.38 (doublet. J=6HZ. Z-methyl). 1.75 (singlet. 11bmethyl). l.83 (singlet. llb-methyl). 4.50 (multiplet. 5-H) ppm (approximately an :15 mixture of the 2- methyl epimers based on peak heights).  
  A solution of 38.3 g. 1.2.5.6.11.1lb-hexahydro- 2.1lb-dimethyl-3fl-pyrido[3.2&#39;:4.5]pyrrolol3.2- g]indolizin-3-one (ca. 85:15 mixture of the Z-methyl epimers) in 900 ml. THF is reduced with 11.38 g. lithium aluminum hydride in a manner similar to that of Example V1. The residue isolated from the methylene chloride extract is recrystallized from ethyl acetate to provide 29.9 g. solid. mp. 185.57 (softens 183). A 14.76 g. portion of this material is chromatographically purified on 450 neutral. activity 111 alumina. Elution of the column with chloroform yields a solid which is thoroughly dried to afford 14.08 g. title compound. mp. l869 (softens 178). NMR (CD0 5 0.88 (doublet. .]=6Hz. 2-methy1). 1.05 (doublet. J=6Hz. 2- methyl). 1.68 (singlet. l lb-methyl). 1.73 (singlet. 11bmethyl). 13.07 (broad singlet. exchangeable. Nfi) ppm (approximately an 86:14 mixture of the 2-methyl epimers based on peak heights).  
  Dissolution of 4.83 g. of the above base in acetone and addition of excess isopropanolic hydrogen chloride yield a salt which is triturated with acetone and thor&#39; oughly dried to provide the title compound (5.26 g.) as the dihydrochloride salt solvated with 0.75 molecule of water per molecule of salt. dec. 269277 (softens 258). A 3.07. 4.17 a. NMR (dDMSO): 5 0.90 (doublet. J=6Hz. 2-methyl). 1.05 (doublet. J=6Hz. 2- methyl). 2.00 (singlet. l lb-methyl). 2.13 (singlet. 11bmethyl) ppm (approximately an 82:18 mixture of the Z-methyl epimers based on peak heights).  
 EXAMPLE XXXVI 2.3.5.6.1l.l1b&#39;Hexahydro 2.ll.llb-Trimethyl-1H- Pyridol3,2&#39;:4.5]Pyrrolol 3.2-gllndolizine Method l) Methylation of 9.65 g 2.3.5.6.]1.l1bhexahydro-2. 1l-dimethyl-lfl-pyrido[3&#39;,2&#39;:4.5]pyrrolo[3.2- g]indolizine (mp. l85.57, softens 183) in 200 ml. dry DMF with 1.92 g. ca. 50 percent sodium hydridemineral oil dispersion and 6.24 g. methyl iodide is conducted in a manner similar to that of Example 111. Method 2. From the methylene chloride extracts of the basic aqueous fraction are isolated 7.98 g. residue which are chromatographically purified on 300 g. neutral. activity 111 alumina. Elution of the column with benzene and with 1:9 chloroform-benzene affords 6.98 g. title compound as a gum. NMR (CDCg): 3 0.95 (doublet. .1=6Hz. 2-methy1) 1.15 (doublet. J=6Hz. 2- methyl). 1.63 (singlet. l lb-methyl). 1.68 (singlet. 11bmethyl). 3.90 (singlet. l l-methyl) ppm (approximately an 84:16 mixture of 2-methyl epimers based on peak heights).  
  Adding excess isopropanolic hydrogen chloride to 6.88 g. of the above base in ether yields a salt which is recrystallized (twice) from methanol-acetone to give 4.80 g. title compound as the dihydrochloride salt. dec. 292-6 (softens 258). NMR (dDMSO and D 01: 1.00 (doublet. J=6Hz. Z-methyl). 1.13 (doublet. J=6Hz. 2-methyl). 2.00 (singlet. llb-methyl). 2.10 (singlet. llb-methyl). 3.95 (singlet. ll-methyl) ppm (approximately an 82:18 mixture of the Z-methyl epimers based on peak heights).  
 Method (2) A 10.21 g. quantity of 1.2.5.6.1 1.1 lb-hexahydro-2. 1lb-dimethyl-3fl-pyridol3.2&#39;:4.5lpyrrolo[3,2 gjindolizin-3-one (mp. 221.5 4&#34;, softens 2l71in 150 ml. dry DMF is methylated with 2.21 g ca. 50 percent sodium hydride-mineral oil dispersion and 6.25 g. methyl iodide in a manner similar to that of Example 111. Method 1. The dichloromethane extract of the basic. aqueous fraction is washed with water. with brine and dried (sodium sulfatel. Removal of solvent yields a gum (9.00 g.) which is chromatographically purified on 300 g. neutral. activity 111 alumina. Development of the column with benzene. with 1:9. 1:4. 1:1 chloro formbenzene and finally with 100 percent chloroform provides 6.96 g. 1.2.5.6. ll.l lb-hexahydro-2.l 1.1 lbtrimethyl-3fl-pyrido[3&#39;.2&#39;:4.5lpyrrolo[3.2-glindolizin- 3one. mp. l25132. lt 5.95 a. NMR (CDCl;,): 8 1.25 (doublet. J=oHz. Z-methyl). 1.40 (doublet. J bHZ. 2-methylJ. 1.65 (singlet. llb-methyl). 1.75 (singlet. llb-methyl). 3.93 (singlet. ll-methyl). 4.53 (multiplet. S-hydrogen) ppm (approximately a 76:24 mixture of isomers based on peak heights).  
  Reduction of 2.0 g. 1.2.5.6.] 1.1 lbhexahydro- 2.11.1lbtrimethyl-Bfl-pyridol3&#39;,2&#39;:4.5]pyrrolo[3.2- glindolizin-3-one (mp. l2532) in ca. 25 ml. THF with 0.57 g. lithium aluminum hydride is conducted in a manner similar to that of Example V1. The yellow gun isolated from the etheral extracts of the basic. aqueous fraction is ehromatographically purified on 80 g. neutral. activity 111 alumina. From the 1:1 hexane-benzene and benzene eluates is isolated the title compound (1.0] gl By 1R. NMR and UV spectral comparisions. this compound (approximately an 89:11 mixture of the 2- methyl epimers based on peak heightsl is the same as that prepared by Method (1) above.  
 EXAMPLE XXXVII The analgesic activity of the compounds of Formula la. lb. 1e. or 1d can be elicited and demonstrated in rats by employing the following procedure:  
  Groups of at least five rats are used. Each rat is positioned so that a high intensity light beam shines on the tip of the tail. The intensity of the beam is adjusted so that a rat will react to the heat so produced by moving his tail out of the beam in 3 to 8 seconds. The average of 3 measurements taken 10 minutes apart serves as a control. The test compound is then administered. and the reaction times are measured every 30 minutes for a 2 hour period. Post drug reaction time is compared to that ofthe control average by statistical analysis. Analgesic activity is indicated by the ability of the compound to prolong the reaction time. The ED is defined as the dose of test material sufficient to cause 50 percent of the test animals to exhibit a positive response. When utilized in the above-described procedure. 2,3,5.6,ll.llb-hexahydrod1,1lb-dimethyl-lflpyrido[3&#39;. 2:4.5lpyrrolol3.2glindolizine. as the dihydrochloride. exhibited an ED of 0.09 mg/kg (P.O.) and 0.12 mg/kg(l.M.):2.3.5.6.11.11b-hexahydro-llb- 28 methyl-1H-pyrido[3&#39;.2:4.5 lpyrrolo[3.Z-glindolizine dihydrochloride. exhibited an ED of 6 mg/ltg(P.O.) and 4.75 mg/kg (l.M.). In this test. morphine exhibits an EDS&#34; of 10 mg/kg (P.O.) and. 2.0 mg/kg (I.M.).  
  The following compounds showed analgesic activity by intraperitoneal administration (with no deaths observed) at the following dosages:  
 (onipountl ol&#39; Dosage (mg/kg) (&#39;onipoimil ts ere tested .i the \ilt de cribed in lhc indlcntcd maniples Number ol rats shouing po lthe tcspttllstil l fll-ll number of rats in le t group lowlsonicr a iliticlnc .It 20 mglltg tlpl lt-sletl as nmture off I cpimcrs ll the 1.1. route. llie HI of morphine l l 5 liiglltg EXAMPLE XXXVIII An injectable solution is prepared from the following formulation:  
 2.35.6.1 1.1lb-l&#39;iexahydro-lLllb- 50 mg. dimethyldfi-pyridol 3&#39;.2&#39;:4.5lp rrolo 13.2-glindolizine. dihy drochloride Sodum acetate. anhydrous (10.15 mg. Phenol 50 mg. Glacial acetic acid qs to pH 5 Water for Injection as. to 50 ml.  
  The phenol and sodium acetate are dissolved in 40 ml. of water. The active ingredient is then dissolved in the solution. The pH of the solution is adjusted with glacial acetic acid. Water for injection is finally added to make a 50 ml. solution. The solution. containing 1 mg/ml of medicament. is subjected to sterile filtration and filled asceptically into sterile vials or ampules.  
 EXAMPLE XXXIX Tablets for oral use are prepared with the following formulation:  
 2.3.5.6.1 l.l lb-hexahydro-l 1.1 lh- 0.5 mg.  
 l3.2-glindoli7ine. dihydrochloridc microcrystalline cellulose. NT. 30.0 mg.  
 magnesium stearate. L&#39;.S.P. 0.3 mg.  
 Amberlite. [RP 88 1.0 mg.  
 Lactose oil] mg. 100.0 mg.