Cephalosporin derivatives
BACKGROUND OF THE INVENTION 
     (1) Field of the Invention 
     The present invention relates to cephalosporin derivatives and their non-toxic salts having excellent antibacterial activity and good oral absorptivity. 
     (2) Prior Art 
     There are disclosed oral cephalosporin derivatives having a 2-carboxymethoxyimino-2-(2-aminothiazol-4-yl)acetamido group at the 7-position of the cephem nucleus (EP patent 210,078A) and having a non-substituted alkylthio group at the 3-position of the cephem nucleus (Japanese Patent Kokai 56-65,095). In addition, among cephalosporin drugs used clinically for oral administration are cephalexin, cefachlor, cefixime and the like. However, these prior art oral cephalosporin derivatives are insufficient in terms of antibacterial activity and antibacterial spectrum against various pathogenic bacteria compared with the cephalosporins developed recently for parenteral administration. 
     Under such circumstances, it is desired to discover new oral cephalosporin drugs having stonger antibacterial activity and wider antibacterial spectrum. 
     SUMMARY OF THE INVENTION 
     As a result of intensive research, the present inventors have discovered novel oral cephalosporin derivatives showing stronger antibacterial activity and wider antibacterial spectrum than the prior art oral cephalosporin drugs. 
     An object of the present invention is to provide a cephalosporin derivative represented by the formula ##STR2## wherein R 1  is a hydrogen atom or a protecting group of the amino group, R 2  is a hydrogen atom or a protecting group of the hydroxyl group, R 3  is a hydrogen atom or a protecting group of the carboxyl group, X is a halogen atom, a cyano group, a vinyl group, a lower alkoxy group having 1 to 4 carbon atoms or a lower alkylthio group having 1 to 4 carbon atoms and n is an integer of 1 to 3, and a non-toxic salt thereof. 
     DETAILED DESCRIPTION OF THE INVENTION 
     In the present invention, the protecting groups of the amino group, hydroxyl group and carboxyl group are those frequently used in the field of the β-lactam chemistry. For example, the protecting groups of the amino group for R 1  may be a trityl group, a monochloroacetyl group, a formyl group, a benzyloxycarbonyl group, a 4-nitrobenzyloxycarbonyl group, a 4-methoxybenzyloxycarbonyl group, a t-butoxycarbonyl group, a trimethylsilyl group and the like, the protecting groups of the hydroxyl group for R 2  may be a formyl group, an acetyl group, a propionyl group, a methoxycarbonyl group, an ethoxycarbonyl group, a butoxycarbonyl group, a t-butoxycarbonyl group, a benzoyl group, a toluoyl group, a benzenesulfonyl group, a tosyl group, a phenylacetyl group, a 4-methoxybenzyl group, 2,4-dimethoxybenzyl group, a trityl group, a tetrahydropyranyl group and the like. The protecting groups of the carboxyl group for R3 may be a methyl group, an ethyl group, a propyl group, an isopropyl group, a butyl group, an isobutyl group, a t-butyl group, a vinyl group, an allyl group, an ethynyl group, a methoxymethyl group, an ethoxymethyl group, a 1-methoxyethyl group, a methylthiomethyl group, a methylthioethyl group, a carboxymethyl group, a carboxyethyl group, a t-butoxycarbonylmethyl group, a 2-(t-butoxycarbonyl)ethyl group, a benzyl group, a 4-nitrobenzyl group, a 4-methoxybenzyl group, a benzhydryl group, a bis-(4-methoxyphenyl)methyl group, a 3,4-dimethoxybenzyl group, a trityl group, a phenethyl group, a phenacyl group, a 2,2,2-trichloroethyl group, a trimethylsilyl group, a 4-hydroxy-3,5 -di(t-butyl)benzyl group, a phenyl group, a tolyl group, a xylyl group and groups known in the pro-drug techniques of the β-lactam drugs such as a 5-methyl-1,3-dioxacyclopent-4-en-2-on-4-ylmethyl group, an acetoxymethyl group, a pivaloyloxymethyl group, a 1-pivaloyloxyethyl group, a 1-acetoxyethyl group, a 3-phthalidyl group, a 1-(ethoxycarbonyloxy)ethyl group, a 1-(cyclohexyloxycarbonyloxy)ethyl group, a 1-(isopropoxycarbonyloxy)ethyl group and the like. 
     The halogen atom for X refers to a fluorine atom, a chlorine atom, a bromine atome and the like, and the lower alkoxy group having 1 to 4 carbon atoms for X refers to a straight or branched chain alkoxy group such as, for example, a methoxy group, an ethoxy group, a n-propoxy group, an isopropoxy group, a n-butoxy group, a t-butoxy group and the like, and the lower alkylthio group having 1 to 4 carbon atoms for X refers to a straight or branched chain alkylthio group such as, for example, a methylthio group, an ethylthio group, a n-propylthio group, an isopropylthio group, a n-butyl group, a t-butylthio group and the like. 
     The non-toxic salt of the compound of Formula I of the present invention refers to pharmacologically acceptable salts, for example, salts with inorganic bases including sodium, potassium, magnesium and ammonium; salts with organic bases such as triethylamine, cyclohexylamine and the like; basic amino acids such as arginine, lysine and the like; and salts with mineral acids such as sulfuric acid, hydrochloric acid, phosphoric acid and the like; and salts with organic acids such as formic acid, acetic acid, lactic acid, tartaric acid, fumaric acid, maleic acid, trifluoroacetic acid, methanesulfonic acid and the like. 
     Among the preferred compounds are compounds of Formula I wherein R 1  and R 2  are each a hydrogen atom, and R 3  is a hydrogen atom or a group known in the pro-drug techniques of the β-lactam drugs such as a pivaloyloxymethyl group, a 1-acetoxyethyl group and the like, and X is a cyano group or a vinyl group. 
     The compounds of Formula I of the present invention are those in the forms of geometric isomers [E-form and Z-form] derived from the oxyimino group at the 7-position side chain, and both isomers are included within the scope of the present invention, but the Z-form is preferred. 
     The compounds of Formura I of the present invention can be, for example, obtained according to a preparation method shown in the following reaction scheme [in the reaction scheme, R 1  and R 2  are as defined above other than a hydrogen atom, R 3 , X and n are as defined above, R 4  is a protecting group of the amino group such as a phenylacetyl group, a phenoxyacetyl group, a trityl group, a phthaloyl group, a formyl group or a benzoyl group, and R 5  is a hydrogen atom or an easily hydrolyzable group in the body which is known in the pro-drug techniques of the β-lactam drugs as described above for R 3 , W and Y are each a halogen atom (e.g., a chlorine atom, a bromine atom and an iodine atom), a methanesulfonyloxy group, a trifluoromethanesulfonyloxy group, a diphenylphosphoryloxy group or a p-toluenesulfonyloxy group]. ##STR3## 
     Process (a): A known compound of Formula II is dissolved in a reaction-inert organic solvent, and the solution together with 1.0 to 1.2 molar equivalents of sodium hydrosulfide in the presence of a base is stirred to give a 3-mercapto derivative. The reaction temperature is from -50° C. to 100° C., preferably from -40° C. to 5° C. The reaction time is from 10 minutes to 4 hours, preferably from 10 minutes to one hour. 
     To the 3-mercapto derivative, obtained by the above procedure, in the same reaction system without isolation, is added to 1.0 to 2.0 molar equivalents of the compound of Formula III, and the mixture is stirred at a reaction temperature of from -50° C. to 100° C., preferably from -25° C. to 50° C., to give a 3-thio substituted derivative of Formula IV. The reaction time depends on the kind of the base used, the kind of the compound of Formula III used and the reaction temperature, but usually it is in the range of 10 minutes to 5 hours, preferably from 10 minutes to 2 hours. 
     The preferred solvents in the above procedure are N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, hexamethylphosphoramide, acetonitrile, tetrahydrofuran, dichloromethane and the like, and they are used alone or in admixture. The preferred bases are organic bases such as diisopropylethylamine, N,N-dimethylaminopyridine, N,N-dimethylaniline, triethylamine and the like. The most preferred amount of the base is from 1.0 to 2.0 molar equivalents relative to the compound of Formula II. 
     The 3-mercapto derivative obtained above, after isolation, can be also provided for the next reaction. Namely, the 3-mercapto derivative is reacted with a compound of Formula III in the presence of a base and a solvent which are the same as those employed above to give a 3-thio substituted derivative of Formula IV. The reaction conditions are also the same as above. 
     Process (b): The protecting group at the 7-position of the compound of Formula IV obtained in Process (a) can be eliminated by a method frequently used in the field of the β-lactam chemistry to give a compound of Formula V. For example, a compound of Formula IV wherein the protecting group R 4  is a phenoxyacetyl group, a phenylacetyl group or a benzoyl group is dissolved in dichloromethane or benzene, and 1.5 to 2.0 molar equivalents of phosphorus pentachloride and 2.0 to 3.0 molar equivalents of pyridine are added, and then the mixture is stirred at -40° C. to 30° C. for 30 minutes to 3 hours. Subsequently, a large excess amount of methanol is added at -50° C. to 20° C., and the mixture is stirred for 30 minutes to 2 hours. After addition of a large excess amount of water, the mixture is stirred at -50° C. to 20° C. for 30 minutes to 1 hour to give the compound of Formula V. 
     A compound of Formula IV wherein the protecting group R 4  is a trityl group is dissolved in a reaction-inert solvent (e.g., ethyl acetate), 1.0 to 1.5  molar equivalents of p-toluenesulfonic acid monohydrate is added under ice-cooling, and then the mixture is stirred for 1 to 5 hours to give the compound of Formula V in the form of p-toluenesulfonic acid salt. If necessary, the p-toluenesulfonic acid salt is treated with a base to give the compound of Formula V in the form of the free base. 
     Process (c): In order to obtain the compound of Formula VII from the compound of Formula V, the compound of Formula V is reacted with a 2-aminothiazoleacetic acid derivative of Formula VI in the presence of a condensing agent or reacted with a reactive derivative of the compound of Formula VI. Examples of the condensing agent are N,N&#39;-dicyclohexylcarbodiimide, 1-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline, carbonyldiimidazole, diphenylphosphoryl azide, Vilsmeier reagent and the like. Examples of the reactive derivative of the compound of Formula VI are the acid halides (e.g., acid chloride and acid bromide), acid anhydrides (e.g., symmetrical acid anhydrides of the compound of Formula VI, and mixed acid anhydrides with ethyl chlorocarbonate, diphenylphosphoric acid, methanesulfonic acid and the like), and activated esters (e.g., esters with p-nitrophenol, thiophenol, N-hydroxysuccinimide and the like). 
     Referring to the use of a mixed acid anhydride of the compound of Formula VI with methanesulfonic acid as a reactive derivative of the compound of Formula VI, first the compound of Formula VI is dissolved in a reaction-inert solvent, and then 1.0 to 1.1 molar equivalents of methanesulfonyl chloride is added to the solution in the presence of a base at -70° C. to -30° C., and the mixture is stirred for 20 to 40 minutes to prepare a mixed acid anhydride of the compound of Formula VI. To the reaction mixture is added a solution of 0.5 to 0.7 molar equivalent of the compound of Formula V in the presence of a base at a temperature of from -70° C. to -30° C., and the mixture is stirred for 20 to 40 minutes to give the compound of Formula VII. Preferred solvents in this process are N,N-dimethylformamide, tetrahydrofuran, acetonitrile, dichloromethane, chloroform and the like. Preferred bases are N,N-diisopropylethylamine, triethylamine, N,N-dimethylaniline, N,N-dimethylaminopyridine, pyridine and the like. The amount of the base used is 1.0 to 2.2 molar equivalents relative to the compound of Formula V. 
     When an acid chloride as a reactive derivative of the compound of Formula VI is used, first the compound of Formula VI is dissolved in a reaction-inert solvent, and then 1.0 to 1.1 molar equivalents of phosphorus pentachloride is added to the solution in the presence of a base at a temperature of from -30° C. to -10° C., and the mixture is stirred for 10 to 30 minutes to prepare an acid chloride of the compound of Formula VI. To the reaction mixture is added a solution of 0.7 to 1.0 molar equivalent of the compound of Formula V in the same reaction-inert solvent as those mentioned above at a temperature ranging from -30° C. to 0° C., and the mixture is stirred for 10 to 30 minutes to give the compound of Formula VII. Preferred solvents in this process are dichloromethane, chloroform, acetonitrile, N,N-dimethylformamide and the like. Preferred bases are pyridine, triethylamine, N,N-dimethylaminopyridine, N,N-dimethylaniline, diisopropylethylamine and the like. The amount of the base used is 4.0 to 5.5 molar equivalents relative to the compound of Formula V. 
     Process (d): In order to obtain the compound of Formula VIII from the compound of Formula VII, the protecting groups of the compound of Formula VII are eliminated by hydrolysis or reduction frequently used in the field of the β-lactam chemistry. 
     Hydrolysis is preferably carried out in the presence of an acid. Examples of the acid are inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid and the like, organic acids such as formic acid, acetic acid, trifluoroacetic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like. Lewis acids such as boron trifluoride, boron trifluoride etherate, aluminium trichloride, antimony pentachloride, ferric chloride, stannic chloride, titanium tetrachloride, zinc chloride and the like and acidic ion exchange resins can be used instead of the acid described above. Furthermore, when the above organic acid or Lewis acid is used, hydrolysis is preferably carried out in the presence of a cation trapping agent such as anisole. Examples of the preferred solvent in hydrolysis are water, methanol, ethanol, tetrahydrofuran, N,N-dimethylformamide, dioxane, dichloromethane, nitromethane and the like, and they are used alone or in admixture. When the acid described above is liquid, it can itself be used as a solvent. 
     Reduction may be carried out by a method of chemical reduction and catalytic reduction. Reducing agents used in the chemical reduction may be a combination of a metal such as zinc, iron and the like, with an organic or inorganic acid such as formic acid, acetic acid, trifluoroacetic acid, hydrochloric acid, hydrobromic acid and the like. 
     Examples of the catalyst used in the catalytic reduction are platinum black, platinum oxide, palladium black, palladium on carbon, Raney nickel and the like. 
     Usually, reduction may be carried out in a conventional solvent which is unaffected disadvantageously to the reaction such as water, methanol, ethanol, acetic acid, tetrahydrofuran, N,N-dimethylformamide, dioxane and a mixture thereof. 
     The reaction temperature of the above hydrolysis or reduction is not restricted, but usually the reaction is carried out under cooling or warming. 
     The compounds of Formula I and their non-toxic salts of the present invention show not only strong antibacterial activity against various pathogenic bacteria containing gram positive bacteria and gram negative bacteria, but also good oral absorption, and therefore, they are useful as antibacterial agents for oral administration. 
     For the purposes, the compound of the present invention may be administered orally in a conventional form such as tablets, pills, capsules, granules and the like, all of which can be prepared according to conventional pharmaceutical practices. In the above preparations are used conventional additives such as fillers, binding agents, disintegrators, pH adjusting agents, solubilizers and the like. 
     The dosage of the compound of the present invention depends on the age of the patient and the kind and conditions of the disease, but usually it is from 10 to 3000 mg in single or several divided doses per day. 
     Subsequently, the results of the antibacterial activity test and oral absorption test of the compounds of the present invention are shown below. 
     Experiment 1 
     The antibacterial activity against various bacteria was measured by the agar plate dilution method (inoculum size: 10 6  cells/ml), and the results are shown in the following Table 1. 
     
                       TABLE 1                                                     
______________________________________                                    
Minimum Inhibitory Concentration (MIC: μg/ml)                          
         Test drug                                                        
Bacteria   A       B       C     D      E                                 
______________________________________                                    
Staphylococcus                                                            
           0.20    0.39    0.20  0.78   12.5                              
aureus 209P-JC                                                            
Staphylococcus                                                            
           0.78    0.78    6.25  &gt;100   &gt;100                              
aureus K-1                                                                
Staphylococcus                                                            
           0.78    0.78    0.39  1.56   50                                
epidermidis sp-al-1                                                       
Entercococcus                                                             
           1.56    25      12.5  100    &gt;100                              
faecalis ATCC 8043                                                        
Eschelichia coli                                                          
           0.20    0.39    0.20  6.25   0.39                              
NIHJ JC-2                                                                 
Eschelichia                                                               
           1.56    6.25    6.25  &gt;100   &gt;100                              
coli A13                                                                  
______________________________________                                    
 Note                                                                     
 A: The compound obtained in Example 1(d) (sodium salt)                   
 B: The compound obtained in Example 2(d) (sodium salt)                   
 C: The compound obtained in Example 5(d) (sodium salt)                   
 D: Cephalexin (known compound)                                           
 E: Cefixime sodium salt (known compound)                                 
 
    
     Experiment 2 
     Three male Japanese native strain white rabbits weighing 2.50 to 3.15 kg for each group were administered orally with the test compounds, and the change of the concentration of the compound in blood was measured. 
     Dosage of the test compound: 20 mg/kg (suspended in 5% gum arabic). 
     Quantitative method: Bioassy method (test bacterium: Bacillus luteus S1101). 
     The results are shown in Table 2. 
     
                       TABLE 2                                                     
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Concentration in blood (μg/ml)                                         
Time after          Test compound                                         
administration      A     D                                               
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One Hour            2.5   5.5                                             
Two Hours           4.0   2.9                                             
Four Hours          2.5   0.6                                             
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 Note                                                                     
 A and D are as defined in Table 1.                                       
 
    
    
    
     The present invention is illustrated by the following Examples in more detail. 
     EXAMPLE 1 
     (a) To a solution of 1000 mg (1.9 mM) of 4-methoxybenzyl 7β-phenylacetamido-3-methanesulfonyloxy-3-cephem-4-carboxylate in 12 ml of N,N-dimethylformamide were added at -10° C. a solution of 165 mg (2.0 mM) of 70% sodium hydrosulfide in 7 ml of N,N-dimethylformamide and 364 mg (2.8 mM) of diisopropylethylamine, and the mixture was stirred at the same temperature for 30 minutes. To the mixture was added 170 mg (3.8 mM) of chloroacetonitrile, and the mixture was stirred at 0° C. for 15 minutes. After the reaction, 100 ml of ethyl acetate was added, and the mixture was washed with, in turn, 50 ml of 0.5% hydrochloric acid and 50 ml of a saturated aqueous sodium chloride solution, and dried over anhydrous magnesium sulfate. The solvent was evaporated and the residue was chromatographed on silica gel column (eluent; benzene:ethyl acetate=5:1) to give 848 mg of 4-methoxybenzyl 7β-phenylacetamide-3-cyanomethylthio-3-cephem-4-carboxylate. 
     NMR (CDCl 3 ) δ(ppm); 3.30 (1H, d, J=17 Hz), 3.52˜3.74 (5H, m), 3.80 (3H, s), 4.96 (1H, d, J=5 Hz), 5.24 (2H, s), 5.85 (1H, dd, J=5 Hz, 9 Hz), 6.30 (1H, d, J=9 Hz), 6.89 (2H, d, J=9 Hz), 7.22˜7.44 (7H, m). 
     IR ν max   KBr  cm -1  ; 3240, 1775, 1650, 1510, 1350, 1230, 1165. 
     (b) To a cooled (0° C.) solution of 720 mg (1.41 mM) of 4-methoxybenzyl 7β-phenylacetamide-3-cyanomethylthio-3-cephem-4-carboxylate, obtained in the above (a), in 15 ml of anhydrous dichloromethane were added 338 mg (4.23 mM) of pyridine and 588 mg (2.83 mM) of phosphorus pentachloride, and the reaction temperature was raised to room temperature for a period of 30 minutes, and the mixture was stirred at the same temperature for 1 hour. To the cooled (-50° C.) reaction solution was added 7.5 ml of anhydrous methanol, and the mixture was stirred at -20° C. for 1 hour. Upon continued cooling of the reaction mixture to -50° C., 7.5 ml of water was added, and the mixture was stirred under ice-cooling for 40 minutes. The mixture was made weakly basic by addition of a saturated aqueous sodium bicarbonate solution and extracted with 100 ml of dichloromethane, and the extract was washed with 50 ml of a saturated aqueous sodium chloride solution and dried over anhydrous magnesium sulfate. The solvent was evaporated, and the residue was chromatographed on silica gel column (eluent; dichloromethane:ethyl acetate=2:1) to give 332 mg of 4-methoxybenzyl 7β-amino-3-cyanomethylthio-3-cephem-4-carboxylate. 
     NMR (CDCl 3 ) δ(ppm); 1.78 (2H, bs), 3.30 (1H, d, J=17 Hz), 3.60 (1H, d, J=17 Hz), 3.68 (1H, d, J=18 Hz), 3.82 (3H, s), 3.83 (1H, d, J=18 Hz), 4.79 (1H, d, J=5 Hz), 4.97 (1H, d, J=5 Hz), 5.28 (2H, s), 6.91 (2H, d, J=9 Hz), 7.38 (2H, d, J=9 Hz). 
     IR ν max   KBr  cm -1  ; 3400, 3320, 1690, 1360, 1250, 1230, 1170. 
     (c) To a cooled (-15° C.) solution of 500 mg (0.86 mM) of 2-(2-tritylaminothiazol-4-yl)-2-[(Z)-2,4-dimethoxybenzyloxyimino]acetic acid in 20 ml of anhydrous dichloromethane were added 210 mg (2.6 mM) of pyridine and 180 mg (0.86 mM) of phosphorus pentachloride, and the mixture was stirred for 15 minutes. Then, a solution of 260 mg (0.66 mM) of the 7-amino derivative, obtained in the above (b), in 1 ml of anhydrous dichloromethane was added to the mixture at the same temperature, and the mixture was stirred at -10° to -5° C. for 20 minutes. After the reaction, 50 ml of ethyl acetate was added, and the mixture was washed with, in turn, 20 ml of 5% hydrochloric acid and 30 ml of a saturated aqueous sodium chloride solution and dried over anhydrous magnesium sulfate. The solvent was evaporated and the residue was chromatographed on silica gel column (eluent; benzene:ethyl acetate=5:1) to give 320 mg of 4-methoxybenzyl 7β-{2-(2-tritylaminothizol-4-yl)-2-[(Z)-2,4-dimethoxybenzyloxyimino]acetamido}-3-cyanomethylthio-3-cephem-4-carboxylate. 
     NMR (CDCl 3 ) δ(ppm); 3.32 (1H, d, J=17 Hz), 3.35 (1H, d, J=17 Hz), 3.58 (1H, d, J=17 Hz), 3.61 (1H, d, J=17 Hz), 3.73 (3H, s), 3.81 (6H, s), 4.94 (1H, d, J=5 Hz), 5.25 (2H, s), 5.25 (1H, d, J=10 Hz), 5.35 (1H, d, J=10 Hz), 5.84 (1H, dd, J=5 Hz, 9 Hz), 6.40˜6.49 (2H, m), 6.83 (1H, s), 6.90 (2H, d, J=9 Hz), 7.00 (1H, bs), 7.20˜7.40 (19H, m). 
     IR ν max   KBr  cm -1  ; 3380, 2970, 1790, 1730, 1680, 1615, 1515, 1362, 1290, 1210, 1160, 1090. 
     (d) To a mixture of 4 ml of trifluoroacetic acid and 0.8 ml of anisole was added under ice-cooling 300 mg (0.31 mM) of 4-methoxybenzyl 7β-{2-(2-tritylaminothiazol-4-yl)-2-[(Z)-2,4-dimethoxybenzyloxyimino]acetamido}-3-cyanomethylthio-3-cephem-4-carboxylate, obtained in the above (c), and the mixture was stirred for 45 minutes and then at room temperature for 10 minutes. After the reaction, the reaction solution was slowly added dropwise to a mixture of diethyl ether and n-hexane (1:2, 40 ml), and the formed crystals were collected by filtration to give 150 mg of 7β-{2-(2-aminothiazol-4-yl)-2-[(Z)-hydroxyimino]acetamido}-3-cyanomethylthio-3-cephem-4-carboxylic acid trifluoroacetate. Then, the crystals and 79 mg (0.94 mM) of sodium bicarbonate were dissolved in 5 ml of water, and the solution was chromatographed on Sephadex LH-20 column (eluent; water) to give 108 mg of 7β-{2-(2-aminothiazol-4-yl)-2-[(Z)-hydroxyimino]acetamido}-3-cyanomethylthio-3-cephem-4-carboxylic acid sodium salt. 
     NMR (D 2  O) δ(ppm); 3.65 (1H, d, J=17 Hz), 3.71 (1H, d, J=17 Hz), 3.82 (1H, ds, J=17 Hz), 3.97 (1H, d, J=17 Hz), 5.33 (1H, d, J=5 Hz), 5.88 (1H, d, J=5 Hz), 7.02 (1H, s). 
     IR ν max   KBr  cm -1  ; 3415, 3200, 2250, 1765, 1675, 1615, 1530, 1350, 1265, 1185. 
     Then, a solution of 100 mg of the sodium salt obtained in the above in 3 ml of water was adjusted to pH 2.5 by addition of 10% hydrochloric acid under ice-cooling, and the formed crystals were collected by filtration to give 75 mg of 7β-{2-(2-aminothiazol-4-yl)-2-[(Z)-hydroxyimino]actamido}-3-cyanomethylthio-3-cephem-4-carboxylic acid. 
     NMR (DMSO-d 6 ) δ(ppm); 3.82 (2H, bs), 4.07 (1H, d, J=17 Hz), 4.26 (1H, d, J=17 Hz), 5.23 (1H, d, J=5 Hz), 5.79 (1H, dd, J=5 Hz, 9 Hz), 6.68 (1H, s), 7.15 (2H, bs), 9.51 (1H, d, J=9 Hz), 11.33 (1H, s). 
     IR ν max   KBr  cm -1  ; 3340, 2250, 1770, 1635, 1535, 1390, 1350, 1265, 1185, 1010. 
     Following the procedures and reaction conditions of Example 1(a), there were obtained the following compounds shown in Table 3. 
     In the following Tables, G is a phenylacetamido group, DMB is a 2,4-dimethoxybenzyl group, PMB is a 4-methoxybenzyl group, POM is a pivaloyloxymethyl group and Tr is a trityl group. 
     
                                           TABLE 3                                 
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 ##STR4##                                                                 
Example            NMR (CDCl.sub.3)                                       
No.  R.sup.a  R.sup.b                                                     
                   δ(ppm)  IR ν.sup.KBr .sub.max                 
__________________________________________________________________________
                                 cm.sup.-1                                
2(a) CH.sub.2 CH.sub.2 F                                                  
              PMB  2.80˜3.14 (2H, m)                                
                                 3260                                     
                   3.45 (1H, d, J=17Hz)                                   
                                 1770                                     
                   3.60 (1H, d, J=17Hz)                                   
                                 1700                                     
                   3.64 (2H, s)  1650                                     
                   3.80 (3H, s)  1510                                     
                   4.33˜4.68 (2H, m)                                
                                 1355                                     
                   4.94 (1H, d, J=5Hz)                                    
                                 1240                                     
                   5.22 (2H, s)  1170                                     
                   5.76 (1H, dd, J=5Hz, 9Hz)                              
                   6.33 (1H, d, J=9Hz)                                    
                   6.88 (2H, d, J=9Hz)                                    
                   7.24˜7.42 (7H, m)                                
3(a) CH.sub.2 SCH.sub.3                                                   
              PMB  2.15 (3H, s)  3265                                     
                   3.60 (1H, d, J=13Hz)                                   
                                 2960                                     
                   3.63 (2H, s)  1775                                     
                   3.72 (1H, d, J=17Hz)                                   
                                 1655                                     
                   3.78 (1H, d, J=13Hz)                                   
                                 1615                                     
                   3.79 (3H, s)  1585                                     
                   3.86 (1H, d, J=17Hz)                                   
                                 1535                                     
                   4.95 (1H, d, J=5Hz)                                    
                                 1515                                     
                   5.20 (2H, s)  1495                                     
                   5.77 (1H, dd, J=5Hz, 9Hz)                              
                                 1390                                     
                   6.19 (1H, d, J=9Hz)                                    
                                 1360                                     
                   6.87 (2H, d, J=9Hz)                                    
                                 1285                                     
                   7.22˜7.40 (7H, m)                                
                                 1250                                     
                                 1220                                     
                                 1170                                     
4(a) CH.sub.2 CH.sub.2 OCH.sub.3                                          
              PMB  2.89 (2H, t, J=6Hz)                                    
                                 3275                                     
                   3.30 (3H, s)  1775                                     
                   3.46 (1H, d, J=18Hz)                                   
                                 1710                                     
                   3.51 (2H, t, J=6Hz)                                    
                                 1695                                     
                   3.59 (1H, d, J=15Hz)                                   
                                 1655                                     
                   3.63 (1H, d, J=18Hz)                                   
                                 1645                                     
                   3.68 (1H, d, J=15Hz)                                   
                                 1530                                     
                   3.79 (3H, s)  1520                                     
                   4.92 (1H, d, J=5Hz)                                    
                                 1360                                     
                   5.20 (2H, s)  1225                                     
                   5.73 (1H, dd, J=5Hz, 9Hz)                              
                                 1170                                     
                   6.18 (1H, d, J=9Hz)                                    
                                 1110                                     
                   6.86 (2H, d, J=9Hz)                                    
                   7.22˜7.42 (7H, m)                                
5(a) CH.sub.2 CHCH.sub.2                                                  
              PMB  3.37 (2H, t, J=7Hz)                                    
                                 3325                                     
                   3.49 (2H, bs) 2955                                     
                   3.60 (2H, s)  1755                                     
                   3.77 (3H, s)  1690                                     
                   4.87 (1H, d, J=5Hz)                                    
                                 1615                                     
                   5.10˜5.24 (2H, m)                                
                                 1520                                     
                   5.19 (2H, s)  1390                                     
                   5.67˜5.88 (1H, m)                                
                                 1365                                     
                   5.72 (1H, dd, J=5Hz, 9Hz)                              
                                 1245                                     
                   6.68 (1H, d, J=9Hz)                                    
                                 1170                                     
                   6.85 (1H, d, J=9Hz)                                    
                                 1125                                     
                   7.21˜7.39 (7H, m)                                
                                 1030                                     
6(a) CH.sub.2 CN                                                          
              POM  1.22 (9H, s)  3350                                     
                   3.41 (1H, d, J=17Hz)                                   
                                 2975                                     
                   3.61 (1H, d, J=16Hz)                                   
                                 2245                                     
                   3.66 (1H, d, J=17Hz)                                   
                                 1790                                     
                   3.68 (1H, d, J=18Hz)                                   
                                 1755                                     
                   3.69 (1H, d, J=16Hz)                                   
                                 1675                                     
                   3.79 (1H, d, J=18Hz)                                   
                                 1530                                     
                   5.00 (1H, d, J=5Hz)                                    
                                 1370                                     
                   5.84 (1H, d, J=6Hz)                                    
                                 1280                                     
                   5.85 (1H, dd, J=5Hz, 9Hz)                              
                                 1220                                     
                   5.86 (1H, d, J=6Hz)                                    
                                 1110                                     
                   6.10 (1H, d, J=9Hz)                                    
                                 1025                                     
                   7.24˜7.44 (5H, m)                                
__________________________________________________________________________
 
    
     Following the procedures and reaction conditions of Example 1(b) using the compounds obtained in Examples 2(a) to 6(a) of Table 3, there were obtained the corresponding compounds shown in Table 4. 
     
                                           TABLE 4                                 
__________________________________________________________________________
 ##STR5##                                                                 
Example            NMR (CDCl.sub.3)                                       
No.  R.sup.a  R.sup.b                                                     
                   δ(ppm)  IR ν.sup.KBr .sub.max                 
__________________________________________________________________________
                                 cm.sup.-1                                
2(b) CH.sub.2 CH.sub.2 F                                                  
              PMB  1.67 (2H, bs) 3360                                     
                   2.90˜3.12 (2H, m)                                
                                 1760                                     
                   3.51 (1H, d, J=17Hz)                                   
                                 1610                                     
                   3.70 (1H, d, J=17Hz)                                   
                                 1510                                     
                   3.82 (3H, s)  1350                                     
                   4.34˜4.70 (2H, m)                                
                                 1240                                     
                   4.73 (1H, d, J=5Hz)                                    
                                 1210                                     
                   4.96 (1H, d, J=5Hz)                                    
                                 1170                                     
                   5.26 (2H, s)                                           
                   6.91 (2H, d, J=9Hz)                                    
                   7.39 (2H, d, J=9Hz)                                    
3(b) CH.sub.2 SCH.sub.3                                                   
              PMB  1.72 (2H, bs) 3410                                     
                   2.15 (3H, s)  2930                                     
                   3.65 (1H, d, J=17Hz)                                   
                                 1770                                     
                   3.73 (1H, d, J=13Hz)                                   
                                 1705                                     
                   3.78 (1H, d, J=17Hz)                                   
                                 1610                                     
                   3.80 (3H, s)  1515                                     
                   3.88 (1H, d, J=13Hz)                                   
                                 1390                                     
                   4.72 (1H, d, J=5Hz)                                    
                                 1355                                     
                   4.96 (1H, d, J=5Hz)                                    
                                 1250                                     
                   5.21 (1H, d, J=12Hz)                                   
                                 1215                                     
                   5.27 (1H, d, J=12Hz)                                   
                                 1165                                     
                   6.89 (2H, d, J=9Hz)                                    
                                 1115                                     
                   7.37 (2H, d, J=9Hz)                                    
                                 1025                                     
4(b) CH.sub.2 CH.sub.2 OCH.sub.3                                          
              PMB  1.75 (2H, bs) 3390                                     
                   2.90 (2H, t, J=6Hz)                                    
                                 1775                                     
                   3.31 (3H, s)  1730                                     
                   3.50 (2H, t, J=6Hz)                                    
                                 1515                                     
                   3.53 (1H, d, J=18Hz)                                   
                                 1350                                     
                   3.68 (1H, d, J=18Hz)                                   
                                 1250                                     
                   3.80 (3H, s)  1220                                     
                   4.70 (1H, d, J=5Hz)                                    
                                 1175                                     
                   4.93 (1H, d, J=5Hz)                                    
                                 1110                                     
                   5.23 (2H, s)                                           
                   6.88 (2H, d, J=9Hz)                                    
                   7.36 (2H, d, J=9Hz)                                    
5(b) CH.sub.2 CHCH.sub.2                                                  
              PMB  1.75 (2H, bs) 3390                                     
                   3.33˜3.42 (2H, m)                                
                                 2935                                     
                   3.48 (1H, d, J=17Hz)                                   
                                 1775                                     
                   3.64 (1H, d, J=17Hz)                                   
                                 1730                                     
                   3.80 (3H, s)  1615                                     
                   4.69 (1H, d, J=5Hz)                                    
                                 1515                                     
                   4.91 (1H, d, J=5Hz)                                    
                                 1390                                     
                   5.08˜5.30 (2H, m)                                
                                 1350                                     
                   5.70˜5.90 (1H, m)                                
                                 1250                                     
                   6.89 (2H, d, J=9Hz)                                    
                                 1220                                     
                   7.36 (2H, d, J=9Hz)                                    
                                 1175                                     
                                 1115                                     
                                 1030                                     
6(b) CH.sub.2 CN                                                          
              POM  1.23 (9H, s)  3400                                     
                   1.86 (2H, bs) 2975                                     
                   3.41 (1H, d, J=17Hz)                                   
                                 2245                                     
                   3.70 (1H, d, J=17Hz)                                   
                                 1780                                     
                   3.74 (1H, d, J=17Hz)                                   
                                 1755                                     
                   3.85 (1H, d, J=17Hz)                                   
                                 1615                                     
                   4.82 (1H, d, J=5Hz)                                    
                                 1480                                     
                   4.91 (1H, d, J=5Hz)                                    
                                 1365                                     
                   5.87 (1H, d, J=6Hz)                                    
                                 1280                                     
                   5.93 (1H, d, J=6Hz)                                    
                                 1220                                     
                                 1160                                     
                                 1110                                     
                                 1025                                     
__________________________________________________________________________
 
    
     Following the procedures and reaction conditions of Example 1(c) using the compounds obtained in Examples 2(b) to 6(b) of Table 4, there were obtained the corresponding compounds shown in Table 5. 
     
                                           TABLE 5                                 
__________________________________________________________________________
 ##STR6##                                                                 
Example            NMR (CDCl.sub.3)                                       
No.  R.sup.a  R.sup.b                                                     
                   δ(ppm)  IR ν.sup.KBr .sub.max                 
__________________________________________________________________________
                                 cm.sup.-1                                
2(c) CH.sub.2 CH.sub.2 F                                                  
              PMB  2.92˜3.11 (2H, m)                                
                                 3380                                     
                   3.13 (1H, d, J=17Hz)                                   
                                 2935                                     
                   3.45 (1H, d, J=17Hz)                                   
                                 1785                                     
                   3.74 (3H, s)  1730                                     
                   3.81 (3H, s)  1680                                     
                   3.82 (3H, s)  1615                                     
                   4.37˜4.47 (1H, m)                                
                                 1590                                     
                   4.60˜4.70 (1H, m)                                
                                 1515                                     
                   4.93 (1H, d, J=5Hz)                                    
                                 1450                                     
                   5.21 (1H, d, J=11Hz)                                   
                                 1360                                     
                   5.26 (1H, d, J=10Hz)                                   
                                 1290                                     
                   5.28 (1H, d, J=11Hz)                                   
                                 1210                                     
                   5.36 (1H, d, J=10Hz)                                   
                                 1160                                     
                   5.76 (1H, dd, J=5Hz, 9Hz)                              
                                 1035                                     
                   6.38˜6.50 (2H, m)                                
                   6.87 (1H, s)                                           
                   6.91 (2H, d, J=9Hz)                                    
                   7.01 (1H, bs)                                          
                   7.23˜7.40 (19H, m)                               
3(c) CH.sub.2 SCH.sub.3                                                   
              PMB  2.17 (3H, s)  3395                                     
                   3.25 (1H, d, J=17Hz)                                   
                                 2935                                     
                   3.55 (1H, d, J=17Hz)                                   
                                 1785                                     
                   3.73 (1H, d, J=13Hz)                                   
                                 1685                                     
                   3.73 (3H, s)  1615                                     
                   3.80 (6H, s)  1590                                     
                   3.86 (1H, d, J=13Hz)                                   
                                 1515                                     
                   4.93 (1H, d, J=5Hz)                                    
                                 1360                                     
                   5.19 (1H, d, J=12Hz)                                   
                                 1290                                     
                   5.24 (1H, d, J=11Hz)                                   
                                 1210                                     
                   5.26 (1H, d, J=12Hz)                                   
                                 1160                                     
                   5.34 (2H, d, J=11Hz)                                   
                                 1035                                     
                   5.75 (1H, dd, J=5Hz, 9Hz)                              
                   6.39˜6.47 (2H, m)                                
                   6.84 (1H, s)                                           
                   6.88 (2H, d, J=9Hz)                                    
                   6.98 (1H, bs)                                          
                   7.22˜7.38 (19H, m)                               
4(c) CH.sub.2 CH.sub.2 OCH.sub.3                                          
              PMB  2.86˜2.95 (2H, m)                                
                                 3380                                     
                   3.13 (1H, d, J=18Hz)                                   
                                 1785                                     
                   3.32 (3H, s)  1685                                     
                   3.45 (1H, d, J=18Hz)                                   
                                 1615                                     
                   3.47˜3.55 (2H, m)                                
                                 1515                                     
                   3.74 (3H, s)  1290                                     
                   3.79 (3H, s)  1250                                     
                   3.80 (3H, s)  1210                                     
                   4.92 (1H, d, J=5Hz)                                    
                                 1160                                     
                   5.17 (1H, d, J=12Hz)                                   
                                 1115                                     
                   5.24 (1H, d, J=12Hz)                                   
                                 1035                                     
                   5.25 (1H, d, J=10Hz)                                   
                   5.34 (1H, d, J=10Hz)                                   
                   5.70 (1H, dd, J=5Hz, 9Hz)                              
                   6.37˜6.46 (2H, m)                                
                   6.85 (1H, s)                                           
                   6.88 (2H, d, J=9Hz)                                    
                   6.98 (1H, bs)                                          
                   7.20˜7.38 (19H, m)                               
5(c) CH.sub.2 CHCH.sub.2                                                  
              PMB  3.10 (1H, d, J=17Hz)                                   
                                 3410                                     
                   3.39 (1H, d, J=17Hz)                                   
                                 2935                                     
                   3.31˜3.42 (2H, m)                                
                                 1785                                     
                   3.73 (3H, s)  1685                                     
                   3.80 (6H, s)  1615                                     
                   4.89 (1H, d, J=5Hz)                                    
                                 1590                                     
                   5.10˜5.30 (4H, m)                                
                                 1515                                     
                   5.24 (1H, d, J=11Hz)                                   
                                 1450                                     
                   5.34 (1H, d, J=11Hz)                                   
                                 1360                                     
                   5.70 (1H, dd, J=5Hz, 9Hz)                              
                                 1210                                     
                   5.70˜5.90 (1H, m)                                
                                 1160                                     
                   6.38˜6.47 (2H, m)                                
                                 1035                                     
                   6.85 (1H, s)                                           
                   6.88 (2H, d, J=9Hz)                                    
                   6.98 (1H, bs)                                          
                   7.23˜7.40 (19H, m)                               
6(c) CH.sub.2 CN                                                          
              POM  1.23 (9H, s)  3370                                     
                   3.33 (1H, d, J=17Hz)                                   
                                 2970                                     
                   3.43 (1H, d, J=16Hz)                                   
                                 2245                                     
                   3.62 (1H, d, J=17Hz)                                   
                                 1790                                     
                   3.65 (1H, d, J=16Hz)                                   
                                 1755                                     
                   3.75 (3H, s)  1685                                     
                   3.82 (3H, s)  1615                                     
                   4.97 (1H, d, J=5Hz)                                    
                                 1590                                     
                   5.24 (1H, d, J=10Hz)                                   
                                 1510                                     
                   5.35 (1H, d, J=10Hz)                                   
                                 1370                                     
                   5.86 (1H, dd, J=5Hz, 9Hz)                              
                                 1290                                     
                   5.88 (1H, d, J=6Hz)                                    
                                 1210                                     
                   5.94 (1H, d, J=6Hz)                                    
                                 1160                                     
                   6.40˜6.49 (2H, m)                                
                                 1110                                     
                   6.83 (1H, s)  1030                                     
                   6.99 (1H, bs)                                          
                   7.23˜7.36 (17H, m)                               
__________________________________________________________________________
 
    
     Following the procedures and reaction conditions of Example 1(d) using the compounds obtained in Examples 2(c) to 6(c) of Table 5, there were obtained the corresponding compounds shown in Table 6. 
     
                                           TABLE 6                                 
__________________________________________________________________________
 ##STR7##                                                                 
Example            NMR                                                    
No.  R.sup.a  R.sup.b                                                     
                   δ(ppm)  IR ν.sup.KBr .sub.max                 
__________________________________________________________________________
                                 cm.sup.-1                                
2(d) CH.sub.2 CH.sub.2 F                                                  
              Na   3.03 (1H, dt, J=2Hz, 7Hz)                              
                                 3420                                     
                   3.15 (1H, dt, J=2Hz, 7Hz)                              
                                 1765                                     
                   3.57 (1H, d, J=17Hz)                                   
                                 1680                                     
                   3.87 (1H, d, J=17Hz)                                   
                                 1615                                     
                   4.51 (1H, t, J=7Hz)                                    
                                 1535                                     
                   4.74 (1H, t, J=7Hz)                                    
                                 1400                                     
                   5.28 (1H, d, J=5Hz)                                    
                                 1360                                     
                   5.84 (1H, d, J=5Hz)                                    
                                 1210                                     
                   7.02 (1H, s)  1140                                     
                   (measured solvent, D.sub.2 O)                          
                                 1050                                     
3(d) CH.sub.2 SCH.sub.3                                                   
              Na   2.15 (3H, s)  3420                                     
                   3.48 (1H, d, J=17Hz)                                   
                                 1760                                     
                   3.68 (1H, d, J=17Hz)                                   
                                 1680                                     
                   3.85 (1H, d, J=13Hz)                                   
                                 1610                                     
                   3.97 (1H, d, J=13Hz)                                   
                                 1530                                     
                   5.00 (1H, d, J=5Hz)                                    
                                 1395                                     
                   5.60 (1H, dd, J=5Hz, 8Hz)                              
                                 1355                                     
                   6.65 (1H, s)  1205                                     
                   7.16 (2H, bs) 1185                                     
                   9.42 (1H, d, J=8Hz)                                    
                                 1135                                     
                   11.42 (1H, s) 1045                                     
                   (measured solvent;                                     
                                 1000                                     
                   DMSO-d.sub.6)                                          
4(d) CH.sub.2 CH.sub.2 OCH.sub.3                                          
              Na   2.82 (2H, t, J=7Hz)                                    
                                 3430                                     
                   3.23 (3H, s)  1765                                     
                   3.30 (1H, d, J=17Hz)                                   
                                 1680                                     
                   3.44 (2H, t, J=7Hz)                                    
                                 1640                                     
                   3.64 (1H, d, J=17Hz)                                   
                                 1535                                     
                   5.02 (1H, d, J=7Hz)                                    
                                 1395                                     
                   5.58 (1H, dd, J=5Hz, 8Hz)                              
                                 1355                                     
                   6.65 (1H, s)  1205                                     
                   7.14 (2H, bs) 1135                                     
                   9.42 (1H, d, J= 8Hz)                                   
                   11.45 (1H, s)                                          
                   (measured solvent;                                     
                   DMSO-d.sub.6)                                          
5(d) CH.sub.2 CHCH.sub.2                                                  
              Na   3.38 (1H, d, J=17Hz)                                   
                                 3425                                     
                   3.59 (1H, d, J=17Hz)                                   
                                 1760                                     
                   4.98 (1H, d, J=5Hz)                                    
                                 1685                                     
                   5.00˜5.23 (2H, m)                                
                                 1615                                     
                   5.58 (1H, dd, J=5Hz, 8Hz)                              
                                 1535                                     
                   5.73˜5.93 (1H, m)                                
                                 1400                                     
                   6.64 (1H, s)  1210                                     
                   7.14 (2H, bs) 1135                                     
                   9.41 (1H, d, J=8Hz)                                    
                                 1050                                     
                   11.46 (1H, s)                                          
                   (measured solvent;                                     
                   DMSO-d.sub.6)                                          
6(d) CH.sub.2 CN                                                          
              POM  1.17 (9H, s)  3345                                     
                   3.89 (2H, bs) 2935                                     
                   4.20 (2H, bs) 2250                                     
                   5.27 (1H, d, J=5Hz)                                    
                                 1785                                     
                   5.70 (1H, d, J=6Hz)                                    
                                 1775                                     
                   5.72 (1H, dd, J=5Hz, 9Hz)                              
                                 1675                                     
                   5.92 (1H, d, J=6Hz)                                    
                                 1615                                     
                   5.69 (1H, s)  1530                                     
                   7.15 (2H, bs) 1370                                     
                   9.53 (1H, d, J=9Hz)                                    
                                 1280                                     
                   11.34 (1H, s) 1220                                     
                   (measured solvent;                                     
                                 1160                                     
                   DMSO-d.sub.6) 1110                                     
                                 1030                                     
__________________________________________________________________________