Preparation and use of iodopropargyl esters of α-amino acid derivatives as antimicrobial agents
This application is a continuation of Ser. No. 07/398,867 filed Aug. 25, 1989, and now abandoned. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to the field of antimicrobial agents. 
     2. Description of the Prior Art 
     Ger. Offen. DE 2515091 (1975), Ciba Geigy AG, discloses microbiocidal anilides which are acylanilides which have an amino acid moiety and an iodopropargyl fragment as shown, but the point of attachment of the iodopropargyl fragment is not directly on the amino acid portion of the molecule. These compounds are N-iodopropargyloxy acid amides. 
     Kokai Tokkyo Koho JP 54/125614 (1979), Shigaken Pharmaceutical Co., Ltd. discloses antimicrobial iodopropargyloxy acid amide derivatives useful for bactericides and agricultural fungicides. 
     U.S. Pat. No. 4,535,088-A (1985) assigned to Shionogi and Co., shows 2-(3-iodo)propargyl-amino-thiazole derivatives as useful as antimicrobials, i.e. antibacterial and antifungal agents. 
     Peptides: Synthesis, Structure and Function; Proc. Am. Pept. Symposium, 7th, (1981), pp 101-4 describes the synthesis of C-iodopropargyl glycine. There is no mention of antibacterial or antifungal activity. 
     SUMMARY OF THE INVENTION 
     Many of the antimicrobials of the prior art have toxicity and/or environmental problems. 
     It is an object of the present invention to provide novel antimicrobial compounds which have improved toxicity profiles and are not harmful to the environment. 
     These objects, and others which will become apparent from the following disclosure, are achieved by the present invention which comprises in one aspect compounds of the formula ##STR3## wherein R 1  can be H, an amine protective group, or ##STR4## 
     R 2 , R 5 , and R 6  are independently selected from H and an amine protective group; 
     R 3  and R 4  are independently selected from H, lower alkyl, aryl, arylalkyl, CH 2  OR, CH 2  SR or CH(CH 3 )OR; 
     R=H, propargyl lower alkyl, arylalkyl or aryl; 
     wherein R 1  and R 2  can be joined to form a ring: and 
     wherein when R 1  and R 2  or R 5  and R 6  are both H, the compound is either in free base form or in salt form. 
     Another aspect of the invention is when said amine protective group is selected from amides, carbamates, imines, enamines, aminoacetals, N-alkyl, N-benzyl, N-sulfenyl or N-sulfonyl. 
     A further aspect of the invention are the compounds in salt form. 
     DETAILED DESCRIPTION OF THE INVENTION AND THE PREFERRED EMBODIMENTS 
     The compounds of the invention have been discovered to be unexpectedly effective antimicrobials. The compounds are designed to biodegrade down to non-toxic amino acid components. 
     The compounds of the invention can have R 1  as an amine protective group. Suitable amine protective groups are amides, carbamates, imines, enamines, aminoacetals, N-alkyl, N-benzyl, N-sulfenyl or N-sulfonyl, and the like. For detailed examples of the suitable amine protective groups, see Greene, T.; &#34;Protective Groups in Organic Synthesis,&#34; John Wiley &amp; Sons, Inc., New York, (1981), pages 218-87. 
     When R 1  and R 2  are H, or when R 1  is structure II and both R 5  and R 6  are H, the compound can be in either free base or salt form. Suitable conjugate acids to form the salts are either organic or inorganic acids, for example hydrochloric acid, sulfuric acid, acetic acid, propionic acid, sorbic acid, trifluoroacetic acid, hydrobromic acid, formic acid, and the like. 
     When R 1  and R 2  or R 5  and R 6  are joined to form a ring, suitable rings are cyclic imide derivatives such as phthaloyl, diphenylmaleoyl, dithiasuccinoyl, and the like. 
     When R 3 , R 4  and R are lower alkyl imides, i.e., phthalimido, diphenylmaleimido, dithiasuccinimido, said alkyl can have 1 to 8, preferably 1 to 4 carbon atoms and can be substituted. When R 3 , R 4  or R are aryl, suitable aryls are phenyl, sustituted phenyl, and the like. 
     When R 3 , R 4  or R are arylalkyl, suitable moities are benzyl, diphenylmethyl, sustituted benzyl, and the like. 
     Some representative compounds include the following: 
     1. N-t-butoxycarbonylglycine iodopropargyl ester 
     2. N-t-butoxycarbonyl-L-alanine iodopropargyl ester 
     3. N-t-butoxycarbonyl-O-benzyl-L-serine iodopropargyl ester 
     4. N-t-butoxycarbonyl-S-benzyl-L-cysteine iodopropargyl ester 
     5. N-t-butoxycarbonyl-O-benzyl-L-threonine iodopropargyl ester 
     6. N-t-butoxycarbonyl-S-propargyl-L-cysteine iodopropargyl ester 
     7. glycine iodopropargyl ester trifluoroacetate 
     8. L-alanine iodopropargyl ester trifluoroacetate 
     9. O-benzyl-L-serine iodopropargyl ester trifluoroacetate 
     10. S-benzyl-L-cysteine iodopropargyl ester trifluoroacetate 
     11. O-benzyl-L-threonine iodopropargyl ester trifluoroacetate 
     12. N-t-butoxycarbonyl-L-alanylglycine iodopropargyl ester 
     13. N-t-butoxycarbonyl-L-alanyl-L-alanine iodopropargyl ester 
     14. N-t-butoxycarbonyl-L-alanyl-O-benzyl-L-serine iodopropargyl ester 
     15. N-t-butoxycarbonyl-L-alanyl-S-benzyl-L-cysteine iodopropargyl ester 
     16. N-t-butoxycarbonyl-L-alanyl-O-benzyl-L-threonine iodopropargyl ester 
     17. L-alanylglycine iodopropargyl ester trifluoroacetate 
     18. L-alanyl-L-alanine iodopropargyl ester trifluoroacetate 
     19. L-alanyl-O-benzyl-L-serine iodopropargyl ester trifluoroacetate 
     20. L-alanyl-S-benzyl-L-cysteine iodopropargyl ester trifluoroacetate 
     21. L-alanyl-O-benzyl-L-threonine iodopropargyl ester trifluoroacetate 
     22. L-alanine iodopropargyl ester 
     23. N-t-butoxycarbonyl-D-alanine iodopropargyl ester 
     24. D-alanine iodopropargyl ester trifuoroacetate 
     25. N-t-butoxycarbonyl-D-alanyl-D-alanine iodopropargyl ester 
     26. N-t-butoxycarbonyl-D-alanyl-L-alanine iodopropargyl ester 
     27. N-t-butoxycarbonylglycyl-L-alanine iodopropargyl ester 
     28. N-acetylglycine iodopropargyl ester 
     29. N-benzoylglycine iodopropargyl ester 
     30. N-phthaloylglycine iodopropargyl ester 
     31. N-benzyloxycarbonylglycine iodopropargyl ester 
     32. N-formylglycine iodopropargyl ester 
     33. N-propionylglycine iodopropargyl ester 
     34. N-octanoylglycine iodopropargyl ester 
     35. N-2,4-hexadienoylglycine iodopropargyl ester 
     
                                           TABLE I                                 
__________________________________________________________________________
STRUCTURES and melting points of the representative compounds             
Compound                                                                  
No    R.sup.1      R.sup.2                                                
                         R.sup.6     R.sup.4                              
                                        R.sup.5                           
                                          R.sup.3   Melting Point         
                                                    (°C.)          
__________________________________________________________________________
 1    (CH.sub.3).sub.3 COCO                                               
                   H                      H         65-68                 
 2    (CH.sub.3).sub.3 COCO                                               
                   H                      CH.sub.3  71-73                 
 3    (CH.sub.3).sub.3 COCO                                               
                   H                      CH.sub.2 OCH.sub.2 Ph           
                                                    oil                   
 4    (CH.sub.3).sub.3 COCO                                               
                   H                      CH.sub.2 SCH.sub.2 Ph           
                                                    84-86.5               
 5    (CH.sub.3).sub.3 COCO                                               
                   H                      CH(CH.sub.3)OCH.sub.2 Ph        
                                                    oil                   
 6    (CH.sub.3).sub.3 COCO                                               
                   H                      CH.sub.2 SCH.sub.2 CCI          
                                                    oil                   
 7    H (trifluoroacetate salt)                                           
                   H                      H         130-131               
 8    H (trifluoroacetate salt)                                           
                   H                      CH.sub.3  oil                   
 9    H (trifluoroacetate salt)                                           
                   H                      CH.sub.2 OCH.sub.2 Ph           
                                                    --                    
10    H (trifluoroacetate salt)                                           
                   H                      CH.sub.2 SCH.sub.2 Ph           
                                                    --                    
11    H (trifluoroacetate salt)                                           
                   H                      CH(CH.sub.3)OCH.sub.2 Ph        
                                                    oil                   
12    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sup.3                             
                                        H H         amorph. solid         
13    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH.sub.3  amorph. solid         
14    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH.sub.2 OCH.sub.2 Ph           
                                                    amorph. solid         
15    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH.sub.2 SCH.sub.2 Ph           
                                                    amorp. solid          
16    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH(CH.sub.3)OCH.sub.2 Ph        
                                                    amorph. solid         
17    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     H (trifluoroacetate salt)                        
                                     CH.sub.3                             
                                        H H         oil                   
18    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     H (trifluoroacetate salt)                        
                                     CH.sub.3                             
                                        H CH.sub.3  oil                   
19    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     H (trifluoroacetate salt)                        
                                     CH.sub.3                             
                                        H CH.sub.2 OCH.sub.2 Ph           
                                                    amorp. solid          
20    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     H (trifluoroacetate salt)                        
                                     CH.sub.3                             
                                        H CH.sub.2 SCH.sub.2 Ph           
                                                    amorp. solid          
21    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     H (trifluoroacetate salt)                        
                                     CH.sub.3                             
                                        H CH(CH.sub.3)OCH.sub.2 Ph        
                                                    oil                   
22    H            H                      CH.sub.3  --                    
23    (CH.sub.3).sub.3 COCO                                               
                   H                      CH.sub.3  --                    
24    H (trifluoroacetate salt)                                           
                   H                      CH.sub.3  oil                   
25    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH.sub.3  amorp. solid          
26    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     CH.sub.3                             
                                        H CH.sub.3  amorp. solid          
27    COCHR.sup.4 NR.sup.5 R.sup.6                                        
                   H     (CH.sub.3).sub.3 COCO                            
                                     H  H CH.sub.3  amorp. solid          
28    CH.sub.3 CO  H                      H         119-121.5             
29    PhCO         H                      H         116-120               
30                                                                        
phthaloyl-                           H  155                               
31    PhCH.sub.2 OCO                                                      
                   H                      H         73-75                 
32    HCO          H                      H         81-85                 
33    CH.sub.3 CH.sub.2 CO                                                
                   H                      H         --                    
34    H(CH.sub.2).sub.7 CO                                                
                   H                      H         87-89                 
35    2,4-sorboyl  H                      H         137-138.5             
__________________________________________________________________________
 
    
     The compounds of the invention wherein R 1  is H or an amine protective group can be prepared by esterification of the appropriately n-protected D- or L-amino acid with iodopropargyl alcohol. 
     A variety of esterification methods may be utilized to prepare the compounds of the invention. For example, a solution or suspension of the n-protected D- or L-amino acid in anhydrous solvents such as dichloromethane, 1,4-dioxane, tetrahydrofuran, dimethoxyethane, toluene or N,N-dimethylformamide is allowed to react with iodopropargyl alcohol in the presence of 4-dimethylaminopyridine and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride. The reaction time usually takes place within a range of 1 to 18 hours. The reaction is usually conducted between 0° and 25° C. 
     Numerous other peptide synthesis coupling reagents may be used as esterification agents. Alternatively, the D- or L-amino acid may be converted to an acid chloride or to an activated ester and then reacted with iodopropargyl alcohol to form the desired ester. Such coupling and activation procedures are discussed in the next &#34;Principles of Peptide Synthesis&#34;, Bodanszky, M.; Springer-Verlag: New York, N.Y., (1984). 
     Iodopropargyl alcohol can be prepared by the procedure described in Bulletin of the Chemical Society of Japan, Ando, T., Shioi, S., Nakagawa, M., (1972), 45, 2611. 
     The n-protected D- or L- amino acid where R 1  =(CH 3 ) 3  COCO, PhCH 2  OCO,CH 3  CO,PhCO,HCO,R 2  =H and R 1 , R 2  =phthaloyl are commercially available. 
     The n-protected D- or L- amino acid where R 1  =CH 3  CH 2  CO, H(CH 2 ) 7  CO, and R 2 , R 3  =H can be prepared via N-acylation of commercially available glycine methyl ester hydrochloride with the corresponding acid chloride in the presence of a base such as 4-dimethylaminopyridine or triethylamine in an appropriate anhydrous solvent such as dichloromethane. 
     The n-protected D- or L- amino acid where R 1  =sorbyl and R 2 , R 3  =H can be prepared via N-acylation of glycine methyl ester hydrochloride with sorbic acid in the presence of a base (triethylamine or 4-dimethylaminopyridine) and a peptide coupling reagent (dicyclohexylcarbodiimide or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) in an appropriate anhydrous solvent (dichloromethane or 1,4-dioxane). The resultant N-acylated glycine methyl ester can be hydrolyzed to the N-protected D- or L- amino acid with lithium hydroxide or sodium hydroxide in aqueous tetrahydrofuran followed by acidification with an aqueous inorganic acid such as potassium hydrogen sulfate or hydrochloric acid. 
     Compound numbers 7-11, and 24, which are in the form of trifluoroacetate salts where R 1 , R 2  =H, R 3  =H, CH 3 , CH 2  OCH 2  Ph, CH 2  SCH 2  Ph, CH(CH 3 )OCH 2  Ph can be prepared from the corresponding N-tert-butoxycarbonyl iodopropargyl ester (compound numbers 1-5, 23) by treatment with trifluoroacetic acid in dichloromethane. 
     Dipeptide compounds wherein R 1  is II can be synthesized as follows: A previously prepared iodopropargyl ester amine trifluoroacetate salt (compound numbers 7-11, 24) can be coupled with readily available N-tert-butoxycarbonyl protected D- or L- amino acids utilizing the various known peptide coupling methods described above. More specifically, to a solution or suspension of iodopropargyl ester amine trifluoroacetate salt (I) in anhydrous solvents such as dichloromethane, 1,4-dioxane, tetrahydrofuran, N,N-dimethylformamide, dimethoxyethane or toluene at a temperature between 0° and 25° C. under inert atmosphere is added a base such as triethylamine, N-methylmorpholine or 4-dimethylaminopyridine, followed by a readily available N-tert-butoxycarbonyl protected D- or L-amino acid, followed by N-hydroxybenzotriazole as a racemization suppression additive and catalyst, followed by the coupling agent such as dicyclohexycarbodiimide or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride. The reaction mixture is then stirred at a temperature between 0° and 25° C. for a time between 1 to 24 hours. The product N-tert-butoxycarbonyl dipeptide iodopropargyl ester is then isolated in the usual manner. 
     The trifluoroacetate salts of the dipeptides, (compound numbers 17-21) can be prepared from the corresponding N-t-butoxycarbonyl dipeptide iodopropargyl esters (II), (compound numbers 12-16) by treatment with trifluoroacetic acid in dichloromethane. 
     As stated above, compositions comprising a compound according to formula I and either an agronomically acceptable carrier, a cosmetic agent, a cutting oil, a soap or synthetic detergent, a stabilizer, a film forming material, or the like have a wide range of utility for protecting against or controlling microorganisms from a wide variety of classes including fungi, bacteria, algae, viruses and yeasts. The preferred utilities of the compositions are to protect wood, paint adhesive, glue, paper, textile, leather, plastics, cardboard, lubricants, cosmetics, food, caulking, feed and industrial cooling water from microorganisms. 
     The following lists specific industries and applications of the compounds or compositions: 
     
         ______________________________________                                    
Industry       Application                                                
______________________________________                                    
Adhesives, sealants                                                       
               adhesives                                                  
               caulks                                                     
               sealants                                                   
agriculture/food chain                                                    
               adjuvant preservation                                      
               agricultural active ingredient                             
               agricultural chemical preservative                         
               agricultural formulations preservation                     
               animal feed preservation                                   
               dairy chemicals                                            
               fertilizer preservation                                    
               food preservation                                          
               food processing chemicals                                  
               grain preservation                                         
               post-harvest produce protection                            
               sugar processing                                           
               tobacco                                                    
Construction products                                                     
               asphalt/concrete                                           
               cement modifiers                                           
               construction products                                      
               roof mastics                                               
               synthetic stucco                                           
               wall mastics                                               
               joint cement                                               
Cosmetics and toiletries                                                  
               cosmetics                                                  
               raw materials for cosmetics, toiletries                    
               toiletries                                                 
Disinfectants, antiseptics                                                
               antiseptic                                                 
               disinfectant                                               
Emulsions, dispersions                                                    
               aqueous dispersions                                        
               dispersed pigments                                         
               latex                                                      
               photographic emulsions                                     
               pigment slurries                                           
               polymer latices                                            
formulated household                                                      
               fabric softeners                                           
products       polishes                                                   
               waxes                                                      
               hand dish detergents                                       
               raw materials                                              
               liquid detergents                                          
               hand soaps                                                 
Industrial processing, misc                                               
               electrodeposition paint, baths, rinses.                    
               electrodeposition pre-treatment, post                      
               rinses                                                     
               Industrial fluids preservation                             
               pasteurization baths                                       
               process aid preservation                                   
Industrial water treatment                                                
               air washers                                                
               cooling towers                                             
               cooling water                                              
               water cooling                                              
               preservation/treatment of wooden                           
               cooling tower slats and structural                         
               members                                                    
               can warmers                                                
               brewery pasteurization                                     
               closed loop water cooling systems                          
Laundry        household laundry products                                 
               laundered goods                                            
               laundry rinse water                                        
               sanitizers-laundry                                         
Leather, leather products                                                 
               leather and hide                                           
               leather and hide products                                  
Lubricants, hydraulic aids                                                
               automotive lubricants and fluids                           
               conveyor lubricants                                        
               greases                                                    
               hydraulic fluids                                           
               lubricants                                                 
Medical devices                                                           
               diagnostic enzymes                                         
               diagnostic kits                                            
               medical devices                                            
Metalworking &amp; related                                                    
               cutting fluids                                             
app&#39;s          metal cleaning                                             
               metalworking fluids                                        
Odor control (active                                                      
               air conditioning                                           
ingredient)    animal bedding                                             
               cat litter                                                 
               chemical toilet prep&#39;ns                                    
               deodorizers                                                
               humidifiers                                                
               industrial deodorants                                      
               sanitary formulations                                      
               toilet bowls                                               
Paints and coatings coating                                               
               emulsions                                                  
               paints                                                     
Paper and wood pulp,                                                      
               absorbant materials of paper and                           
their products wood pulp                                                  
               packaging materials of paper and                           
               wood pulp                                                  
               paper                                                      
               paper products                                             
               paper treatment                                            
               soap wrap                                                  
               wood pulp                                                  
               wood pulp products                                         
paper mill     paper mill slimicides                                      
               pulp and paper slurries                                    
Petroleum refining, fuels                                                 
               aviation fuels (jet fuel, aviation gas)                    
               crude oils                                                 
               burner, diesel and turbine fuel oils                       
               coal slurries                                              
               diesel fuel additives                                      
               diesel fuels                                               
               fuels                                                      
               gasoline                                                   
               heating oils                                               
               hydrocarbons                                               
               kerosene                                                   
               liquefied petroleum gas                                    
               petrochemical feedstocks                                   
               petroleum products, storage,                               
               transportation and production                              
               recycled petroleum products                                
               residual fuel oils                                         
               turbine oils                                               
Photographic chemicals                                                    
               photographic processing - wash                             
and process    water, rinses                                              
               photoprocessing                                            
               photoplate processing chemicals                            
               (developers, stabilizers etc)                              
Printing       fountain solutions (printing)                              
               ink components (pigments, resins,                          
               solvents, etc)                                             
               inks                                                       
Sanitizers (active)                                                       
               sanitizers                                                 
               sanitizers-dairy                                           
               sanitizers-dental                                          
               sanitizers-fermentation                                    
               sanitizers-food preparation                                
               sanitizers-food processing                                 
               sanitizers-medical                                         
               sanitizers-rendering                                       
               sanitizers-veterinary                                      
Soaps, detergents, cleaners                                               
               cleaners                                                   
               detergents                                                 
               household cleaners                                         
               industrial cleaners                                        
               liquid soaps                                               
               oil and grease remover                                     
               powdered soaps                                             
               raw materials for cleaning products                        
               soaps                                                      
               surfactants                                                
Textiles, textile products                                                
               bonded fabrics                                             
               burlap                                                     
               canvas                                                     
               canvas goods                                               
               carpet backing                                             
               carpets                                                    
               clothing                                                   
               coated fabrics                                             
               curtains                                                   
               draperies                                                  
               engineering textiles                                       
               fibers                                                     
               geotextiles                                                
               goods made of textiles                                     
               knitted fabrics                                            
               nets                                                       
               nonwoven fabrics                                           
               rope                                                       
               rugs                                                       
               textile accessories                                        
               textile products                                           
               textiles                                                   
               upholstery                                                 
               woven fabrics                                              
               yarn                                                       
Textile processing                                                        
               dye fixatives                                              
               dyes                                                       
               fiber lubricants                                           
               hand modifiers                                             
               sizes                                                      
               textile processing fluids                                  
Therapeutic (active or                                                    
               animal health/veterinary                                   
preservative)  aquaculture                                                
               dental                                                     
               human health                                               
               pharmaceutical/therapeutic                                 
Water purification                                                        
               charcoal beds                                              
               deionization resins                                        
               filters                                                    
               membranes                                                  
               reverse osmosis membranes                                  
               ultrafilters                                               
               water purification                                         
               water purification pipes, tubing                           
Wood applications                                                         
               lazures (wood stains)                                      
               wood                                                       
               wood products                                              
Miscellaneous  alcohols                                                   
               bedding incorporating water or gels                        
               ceramic                                                    
               contact lens cases-leaching                                
               electronic circuitry                                       
               electronics chemicals                                      
               enzymes-food production                                    
               enzymes                                                    
               enzymes-industrial                                         
               gel cushions                                               
               marine antifoulants                                        
               mildewcides                                                
               wood                                                       
               plastics                                                   
               laundry                                                    
               mining                                                     
               natural rubber latex                                       
               oil field injection waters including                       
               enhanced recover injection fluids,                         
               drilling, fracturing and completion                        
               fluids                                                     
               pipes                                                      
               plastics                                                   
               polymer systems                                            
               polymers and resins (synthetic and                         
               natural                                                    
               reagent preservation                                       
               rubber                                                     
               rubber products                                            
               skin remover                                               
               solid protective/decorative films                          
               stains                                                     
               swimming pools                                             
               waste treatment                                            
               water beds                                                 
______________________________________                                    
 
    
     The amounts of the compound to be used depend on the application. The useful amounts of a particular application are similar to amounts used for other microbiocide compounds. 
     The compound can be used in combination with other microbicides. The term &#34;microbicide&#34; is considered equivalent to &#34;antimicrobial&#34; as used herein. 
     Suitable methods of application of compounds of formula I to control fungi, bacteria, algae, viruses, yeasts, and the like are in amounts and with carriers, etc., as well known in the art. 
    
    
     The following examples are presented to illustrate a few embodiments of the invention, but are not to be considered as limiting. 
     EXAMPLE 1 
     N-t-Butoxycarbonylglycine iodopropargyl ester (Compound #1) 
     To a magnetically stirred solution of N-t-butoxycarbonylglycine (3.00 g., 17.12 mmole) and iodopropargyl alcohol (3.12 g., 17.15 mmole) in anhydrous dichloromethane (40 ml.) cooled to 0° C. with an ice-bath was added 4-dimethylaminopyridine (0.21 g., 1.72 mmole) followed by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (3.61 g., 18.83 mmole). After stirring at 0° C. for 1/2 hour, the reaction mixture was stored in a refrigerator at 6° C. overnight. The reaction mixture was transferred to a separatory funnel and diluted to 100 ml. with dichloromethane, then washed successively with water, 10% aqueous potassium hydrogen sulfate, water, saturated aqueous sodium bicarbonate, water and a final wash with saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to a clear colorless oil which crystallized on standing as N-t-butoxycarbonylglycine iodopropargyl ester (5.60 g., 96.4% yield). m.p.=65°-68° C.  1  H-NMR (200 MHz, DMSO d6 )δ=1.40 ppm, s, 9H; 3.73, d, 2H; 4.86, s, 2H; 7.28, t, 1H. IR (CDCl 3 ) 1165, 1505, 1715, 1760, 2205, 3460 cm -1 . 
     EXAMPLE 2 
     N-t-Butoxycarbonyl-L-alanine iodopropargyl ester (Compound #2) 
     To a magnetically stirred solution of N-t-butoxycarbonyl-L-alanine (5.00 g., 0.026 mole) and iodopropargyl alcohol (4.81 g., 0.026 mole) in anhydrous dichloromethane (100 ml.) cooled to 0° C. with an ice-bath was added 4-dimethylaminopyridine (0.32 g., 0.0026 mole) followed by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (5.57 g., 0.029 mole). The reaction mixture continued to stir at 0° C. for one hour, and was then stored in a refrigerator at 5° C. overnight. The reaction mixture was transferred to a separatory funnel and diluted to double volume (200 ml.) with dichloromethane, then washed successively with 100 ml. of water, 3×100 ml. of 10% aqueous potassium hydrogen sulfate, 100 ml. of water, 3×100 ml. of saturated aqueous sodium bicarbonate, 3×100 ml. of water and a final wash with 100 ml. of saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to a clear colorless oil which crystallized on standing. A quantitative yield (9.34 g.) of N-t-butoxycarbonyl-L-alanine iodopropargyl ester was obtained. m.p.=71°-73° C.  1  H-NMR (200 MHz, DMSO d6 ) δ=1.25 ppm, d, 3H; 1.40, s, 9H; 4.01, m, 1H; 4.85, s, 2H; 7.33, d, 1H. IR (CHCl 3 ) 1170, 1375, 1400, 1715, 1750, 2205 cm -1 . 
     EXAMPLE 3 
     Glycine iodopropargyl ester trifluoroacetate (Compound #7) 
     N-t-Butoxycarbonyl glycine iodopropargyl ester (Compound #1) (4.20 g., 12.39 mmole) was dissolved in anhydrous dichloromethane (20 ml.), cooled to 0° C. with an ice-bath, and treated with 10 ml. of trifluoroacetic acid. The reaction mixture was stirred magnetically at 0° C. for 1 hour and then allowed to warm to ambient temperature. The volatile solvents were removed under reduced pressure leaving a clear syrup which crystallized on standing to a white solid (4.20 g., 96% yield) as the desired trifluoroacetate salt product. m.p. 130°-131° C.  1  H-NMR (200 MHz, DMSO d6 ) δ=3.92, s, 2H; 5.00, s; 2H; s; 9.1, broad m. IR (KBr) 1605, 1670, 1765, 2205, 2640 cm -1 . 
     EXAMPLE 4 
     L-Alanine iodopropargyl ester trifluoroacetate (Compound #8) 
     N-t-Butoxycarbonyl-L-alanine iodopropargyl ester (Compound #2) (3.50 g., 9.91 mmole) was dissolved in anhydrous dichloromethane (20 ml.), cooled to 0° C. with an ice-bath, and treated with 10 ml. of trifluoroacetic acid. The reaction mixture was stirred magnetically at 0°-7° C. for 17 hours and then allowed to warm to ambient temperature. The volatile solvents were removed under reduced pressure leaving a clear colorless syrup as the desired trifluoroacetate salt product.  1  H-NMR (200 MHz, D 2  O) δ=1.58 ppm, d, 3H;4.23, q, 1H; 4.99, s, 2H. IR (CHCl 3 ) 1175, 1675, 1765, 2230, 2250-3500 cm -1 . 
     EXAMPLE 5 
     N-t-Butoxycarbonyl-L-alanylglycine iodopropargyl ester (Compound #12) 
     To a magnetically stirred, anhydrous dichloromethane (20 ml.) solution of glycine iodopropargyl ester trifluoroacetate (Compound #7) (2.00 g., 5.66 mmole) held at 0° C. with an ice-bath was added triethylamine (0.79 ml., 5.67 mmole), followed by N-t-butoxycarbonyl-L-alanine (1.07 g., 5.66 mmole), 1-hydroxybenzotriazole hydrate (0.77 g., 5.70 mmole) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (1.30 g., 6.78 mmole). The reaction mixture was held at 5° C. for 17 hours. The resultant solution was transferred to a separatory funnel and diluted to an approximate volume of 100 ml. with dichloromethane, then washed successively with water, 10% aqueous potassium hydrogen sulfate, water, saturated aqueous sodium bicarbonate, water and a final wash with saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to give 2.09 g. (90% yield) of the desired N-t-butoxycarbonyl-L-alanylglycine iodopropargyl ester as a dry white foam.  1  H-NMR (200 MHz, CDCl 3 ) δ=1.40, d, 3H; 1.47, 1.77, two s, 9H; 4.08, d, 2H; 4.24, m, 1H; 4.90, s, 2H; 5.06, d, 1H; 6.81, s, 1H. IR (CHCl 3 ) 1160, 1375, 1495, 1690, 1755, 2200, 2985, 3440 cm -1 . 
     EXAMPLE 6 
     N-t-Butoxycarbonylglycl-L-alanine iodopropargyl ester (Compound #27) 
     To a magnetically stirred, anhydrous dichloromethane (30 ml.) solution of L-alanine iodopropargyl ester trifluoroacetate (Compound #8) (2.30 g., 6.27 mmole) held at 0° C. with an ice-bath was added triethylamine (0.96 ml., 6.89 mmole), followed by N-t-butoxycarbonylglycine (1.10 g., 6.28 mmole), 1-hydroxybenzotriazole hydrate (0.85 g., 6.29 mmole) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (1.56 g., 8.14 mmole). The reaction mixture was held at 0°-7° C. for 17 hours. The resultant solution was transferred to a separatory funnel and diluted to an approximate volume of 100 ml. with dichloromethane, then washed successively with 3×50 ml. of water, 3×50 ml. of 10% aqueous potassium hydrogen sulfate, 3×50 ml. of saturated aqueous sodium bicarbonate, 3×50 ml. of water and a final wash with 50 ml. of saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to give 1.80 g. (70% yield) of the desired N-t-butoxycarbonylglycyl-L-alanine iodopropargyl ester as a dry white foam.  1  H-NMR (200 MHz, CDCl 3 ) δ=1.45 ppm, d, 3H; 1.45, 1.72, two s, 9H; 3.82, d, 2H; 4.63, m, 1H; 4.87, s, 2H; 5.17, broad m, 1H; 6.67, d, 1H. 
     EXAMPLE 7 
     N-2,4-hexadienoylglycine iodopropargyl ester (Compound #35) 
     To a magnetically stirred mixture of glycine methyl ester hydrochloride (4.14 g., 32.97 mmole) and 4-dimethylaminopyridine (4.03 g., 32.99 mmole) in anhydrous dichloromethane (75 ml.) cooled to 0° C. with an ice-bath was added sorbic acid (3.36 g., 29.97 mmole), followed by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (6.90 g., 35.99 mmole). The reaction mixture continued to stir at 0° C. for 20 minutes. After storing in a refrigerator at 5° C. overnight, the reaction mixture was transferred to a separatory funnel and diluted to double volume (150 ml.) with dichloromethane, then washed successively with 3×40 ml. of water, 3×40 ml. of 10% aqueous potassium hydrogen sulfate, 3×40 ml. of saturated aqueous sodium bicarbonate, 3×40 ml. of water and a final wash with 40 ml. of saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to a fluffy white solid. An 88% yield (4.84 g.) of N-sorboyl methyl ester was obtained. m.p.=121°-122° C.  1  H-NMR (200 MHz, CDCl 3 )δ=1.84 ppm, t, 3H; 3.77, s, 3H; 3.77, s, 3H; 4.15, d, 2H; 5.83, d, 1H; 6.15, m, 3H; 7.24, m, 1H. IR (CHCl 3 ) 1010, 1380, 1445, 1525, 1625, 1645, 1675, 1750, 3000, 3440 cm -1 . 
     Sorboyl methyl ester (4.08 g., 22.27 mmole) was dissolved in 75 ml. of a tetrahydrofuran-water mixture (3:1) and treated with 0.602 g. (24.57 mmole) of lithium hydroxide. After magnetically stirring for three hours, the reaction mixture was concentrated under reduced pressure to a dry residue which was dissolved in a minimum amount of water. With magnetic stirring, this solution was acidified to a pH of 2 with 4.1 ml. (24.6 mmole) of 6N aqueous hydrochloric acid. The desired product sorboyl was isolated by filtration and dried in vacuo over P 2  O 5  to afford 3.13 g. (83% yield). m.p.=164°-166° C.  1  H-NMR (200 MHz, DMSO d6 )δ=1.8 ppm, d, 3H; 3.85, d, 2H; 5.9-6.4, m, 3H; 7.03, dd, 1H; 8.32, t, 1H; 12.6, broad m, 1H. 
     To a magnetically stirred slurry of N-sorbylglycine (2.59 g., 15.31 mmole) and 4-dimethylaminopyridine (0.187 g., 1.53 mmole) in anhydrous dichloromethane (80 ml.) cooled to 0° C. with an ice-bath was added iodopropargyl alcohol (3.06 g., 16.82 mmole) followed by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (4.11 g., 21.44 mmole). The reaction mixture continued to stir at 0° C. and slowly warm to ambient temperature as the ice melted. After stirring for 2.5 hours, the reaction mixture was stored in a refrigerator at 5° C. overnight. The reaction mixture was transferred to a separatory funnel and diluted to approximately 200 ml. with dichloromethane, then washed successively with 2×35 ml. of water, 2×35 ml. of 10% aqueous potassium hydrogen sulfate, 2×35 ml. of saturated aqueous sodium bicarbonate and a final wash with 50 ml. of saturated aqueous sodium chloride. The organic layer was dried with magnesium sulfate, filtered and concentrated in vacuo with a rotary evaporator to an orange solid (4.47 g.). This material was purified by flash column chromatography by dissolving it in dichloromethane and adsorbing it on to 14 g. of silica gel, then removing the solvent under reduced pressure. The resultant dry powder was applied on to the head of a 134 g. silica gel column and eluted with hexanes/ethyl acetate (2:1). Desired product N-sorbogl iodopropargyl ester was obtained as a crystalline solid in 40.4% yield after chromatography. m.p.=137°-138.5° C.  1  H-NMR (200 MHz, CDCl 3 )δ=1.84 ppm, d, 3H; 4.18, d, 2H; 5.80, d, 1H; 5.95-6.30, m, 3H; 7.15-7.30, m, 1H. 
     EXAMPLE 8--BIOLOGICAL ACTIVITY 
     A. Biocidal Activity 
     Biocidal evaluations (bactericidal, algicidal, and fungicidal) were carried out. 
     A minimum inhibitory concentration (MIC) value is obtained using a broth, two-fold serial dilution test performed as follows: A stock solution or dispersion of the test compound, typically at a concentration of 1%, is made in a 5:3:2 solvent solution of acetone, methanol, and water. A volume of the stock solution is dispensed into culture media to give an initial starting test concentration of 500 ppm compound. 
     When the test is ready to be done, each vessel in the dilution series, except the first vessel, contains an equal volume of compound free broth. The first vessel contains twice the volume of broth with the starting concentration of test compound. One half of the broth from the first vessel is transferred to the second vessel. After being mixed, one half the resulting volume is removed from the second vessel and transferred to the third vessel. The entire cycle is repeated sufficiently to give a series of concentrations amounting to 500, 250, 125, 63, 31, 16, 8, and 4 ppm (or 100, 50, 25, 12.5, 6.2, 3.1, 1.6, and 0.8), respectively. 
     Each vessel is then inoculated with a cell suspension of the appropriate test organism. Bacteria are grown in broth, fungi on agar slants for a time and at a temperature appropriate to the species being tested, and algae are a mixture of green algae and blue-green bacteria grown in a nutrient media. At the end of the growth period, in the case of bacteria, the broth is vortexed to disperse the cells. 
     In the case of fungi, the spores are harvested by pipetting water onto the slant and dislodging the spores with a sterile loop. The cell/spore suspension is standardized by controlling incubation time, temperature, and the volume of the diluent. The suspension is then used to inoculate the vessels containing the broth compound. 
     The algae culture contains green algae and blue-green bacteria, and is obtained from a cooling tower in Spring House, Penn. The algae culture is grown in Allen&#39;s medium on a rotary shaker under flourescent room lighting. This culture is further diluted with Allen&#39;s medium and then added to the test vessel. 
     The vessels are then incubated at the appropriate temperature. After the incubation, the vessels are examined for growth/no growth. The minimum inhibitory concentration (MIC) is defined as the lowest concentration of compound that results in complete inhibition of growth of the test organism. 
     The organisms tested to demonstrate biocidal activity include: 
     BACTERIA: 
     Pseudomonas fluorescens (PSFL), gram negative 
     Pseudomonas aerugenosa (PSAE), gram negative 
     Escherichia coli (ECOL), gram negative 
     Staphylococcus aureus (SAUR), gram positive 
     FUNGI: 
     Aspergillus niger (ANIG) 
     Aureobasidium pullulans (APUL) 
     The results of the minimum inhibitory concentration (MIC) tests of compounds of this invention are shown in Tables IIIa, IIIb, IIIc, V, VI, VII, and VIII against the microorganisms shown in Table IX. 
     B. In-Vitro Plant Fungicidal Tests 
     In-vitro tests of plant diseases were carried out. 
     The organisms employed in the test are: 
     PYU Pythium ultimum (Oomycete) 
     PHY Phytophthora capsici (Oomycete) 
     PIR Piricularia oryzae (Ascomycete) 
     HEL Cochliobolus sativus (Ascomycete) 
     BOC Botrytis cinerea (Ascomycete) 
     FUS Fusarium roseum (Ascomycete) 
     SEP Septoria nodorum (Ascomycete) 
     RHI Rhizoctonia solani (Basidiomycete) 
     XAN Xanthomonas campestris (bacterium) 
     Methods: 
     1. Culture maintenance: 
     Transfers in steps 1 and 2 are done in a laminar flow hood. All 8 fungi and the bacterium used in this test are transferred and maintaned on potato dextrose agar plates each week (2 plates/organism). Organisms are used when they are the following ages: a. 1 week old: PYU, PHY, RHI; b. 2 weeks old: XAN, PIR, BOC, HEL, FUS, SEP, COL, MON, CER, UST, ALT; c. 3 weeks old: PSH, VEN. Pythium ultimum and Phytophthora capsici are transferred to asparagine-sucrose broth shake cultures (ASB). Rhizoctonia solani, Fusarium roseum, and Xanthomonas campestris are mainted in yeast extract-dextrose broth (YDB) on a shaker. Culture flasks are inoculated with 6 mycelial plugs each (except for Pythium which is inoculated with only 3 plugs) taken from PDA plates. All liquid shaker cultrues are used after 2 days growth. 
     2. Inoculum preparation. 
     Conidia and mycelium from PIR, BOC, HEL, SEP, COL, MON, CER, PSH, UST and ALT are lightly scraped off into YDB so that mostly conidia are used as inoculum. The conidial suspension is strained through a double layer of cheesecloth to remove mycelial clumps. One plate produces enough conidia or mycelium to inoculate 100 ml of YDB. XAN broth culture is poured (1 ml culture/100 ml broth) into YDB. PYU, PHY, RHI and FUS cultures are ground up (2-3 5 second bursts in a blender) and all but Pythium and Phytophthora are filtered through a dobule layer of sterile cheesecloth to remove large mycelial clumps. Ten ml of the culture solutions of R. solani and F. roseum are added to 90 ml of YSB and 10 ml of the P. capsici is added to 90 ml ASB. Two ml of the culture solution of P. ultimum is added to 98 ml of ASB. Care must be made not to overinoculate (e.g. solutions should appear fairly clear to the eye, yet when held up to light a faint cloudiness should be visible) or standards will not behave properly. The inoculum mixtures are placed in microtiter plates using a 12-tipped pipet. 175 μl (single dose) or 100 μl (dose-response test) of inoculum broth is placed in each well of the microtiter plates. The plates with inoculated media are placed in the refrigerator overnight. There are two replications per treatment. 
     3. Addition of compounds. 
     This operation is carried out in a hood. Six microtiter plates have 245 microliters of sterile water added to their wells ahead of time. 10 Mg a.i. (active ingredient) of the compounds are placed in 1 ml 1:1 acetone:methanol. 5 microliters of this solution is pipetted into the microtiter plates containing the sterile water according to the grid. There are 45 compounds and 3 control treatments per plate. There are 2 replicates per treatment. 25 Microliters of solution is transferred to the inoculated plates with a 96 well replicator. The replicator is flame sterilized with alcohol, rinsed with sterile water, and blotted on sterile paper towells between each transfer. 
     The results of % control of plant fungi at a certain concentration of compounds of this invention are shown in Tables IVa and IVb. 
     
                       TABLE IIIa                                                  
______________________________________                                    
BIOCIDES SECONDARY MIC TEST DATA IN PPM                                   
These compounds were tested in M9G (minimal, salts media                  
with glucose)                                                             
       (G-)     (G-)    (G-)  (G+)  (F)   (F)                             
Cpd #  PSFL     PSAE    ECOL  SAUR  ANIG  APUL                            
______________________________________                                    
1      50       100     &gt;100  100   2     &lt;0.8                            
1      125      16      125   16    1     1                               
1      32       63      32    8     &lt;4    &lt;4                              
2      50       &gt;100    &gt;100  50    3     3                               
3      &gt;100     &gt;100    &gt;100  25    3     &lt;0.8                            
4      &gt;10      &gt;100    &gt;100  25    3     3                               
5      &gt;100     &gt;100    &gt;100  63    25    &lt;0.8                            
6      &gt;100     100     &gt;100  &gt;100  25    32                              
6      500      500     500   64    &lt;4    &lt;4                              
7      13       100     100   &gt;100  3     2                               
7      125      125     64    500   &lt;0.8  &lt;0.8                            
8      125      125     64    500   &lt;4    &lt;4                              
8      25       &gt;100    &gt;100  &gt;100  3     2                               
9      25       125     64    500   &lt;4    &lt;4                              
10     &gt;100     100     &gt;100  &gt;100  3     6.3                             
11     &gt;100     &gt;100    &gt;100  &gt;100  6.3   3                               
22     100      100     100   100   &lt;0.8  &lt;0.8                            
23     63       32      16    63    &lt;4    &lt;4                              
23     16       250     125   32    &lt;4    &lt;4                              
24     63       32      32    125   &lt;4    &lt;4                              
24     32       125     63    63    &lt;4    &lt;4                              
______________________________________                                    
 
    
     
                       TABLE IIIb                                                  
______________________________________                                    
BIOCIDES SECONDARY MIC TEST DATA IN PPM                                   
These compounds were tested in trypticase soy broth (complex              
media)                                                                    
       (G-)     (G-)    (G-)  (G+)  (F)   (F)                             
Cpd #  PSFL     PSAE    ECOL  SAUR  ANIG  APUL                            
______________________________________                                    
28     63       63      63    63    2     4                               
29     32       125     125   32    0.50  .                               
30     &gt;250     &gt;250    &gt;250  &gt;250  0.12  .                               
31     32       63      250   32    2     4                               
32     32       63      63    63    2     8                               
34     63       125     &gt;250  250   &lt;0.12 8                               
35     63       125     125   63    2     4                               
______________________________________                                    
 
    
     
                       TABLE IIIc                                                  
______________________________________                                    
BIOCIDES SECONDARY MIC TEST DATA IN PPM                                   
These compounds were tested in M9G (minimal salts media                   
with glucose)                                                             
       (G-)     (G-)    (G-)  (G+)  (F)   (F)                             
Cpd #  PSFL     PSAE    ECOL  SAUR  ANIG  APUL                            
______________________________________                                    
12     50       50      50    50    6     &lt;0.8                            
13     50       50      100   50    &lt;0.8  &lt;0.8                            
14     100      50      &gt;100  100   6     &lt;0.8                            
15     &gt;100     &gt;100    &gt;100  &gt;100  6     1.6                             
16     &gt;100     &gt;100    &gt;100  6     13    &lt;0.8                            
17     100      100     100   100   &lt;0.8  &lt;0.8                            
18     100      100     100   &gt;100  &lt;0.8  &lt;0.8                            
19     50       50      100   100   &lt;0.8  &lt;0.8                            
20     100      100     100   100   &lt;0.8  &lt;0.8                            
21     100      100     100   &gt;100  &lt;0.8  1.6                             
25     125      63      63    125   &lt;4    &lt;4                              
25     32       250     63    32    &lt;4    &lt;4                              
26     125      63      63    125   &lt;4    &lt;4                              
26     32       250     63    32    16    8                               
26     32       125     32    8     &lt;4    8                               
26     32       125     32    8     &lt; 4   8                               
27     125      250     250   125   &lt;4    8                               
______________________________________                                    
 
    
     
                                           TABLE IVa                               
__________________________________________________________________________
IN-VITRO PLANT FUNGICIDE TEST RESULTS                                     
% Control at 25 ppm                                                       
Compd #                                                                   
     BOC FUS HEL PHY PIR PYU RHI SEP XAN                                  
__________________________________________________________________________
 1   100 100 100 100 100 100 100 100 0                                    
 2   100 100 100 100 100 100 100 100 0                                    
 3   0*  100 100 100 100 100 100 100 0                                    
 4   0*  95  0*  100 100 100 100 100 0                                    
 4   75  100 75  100 100 100 100 100 0                                    
 5   0*  95  0   50  100 0   100 75  0                                    
 5   50  95  75  50  100 0   100 90  0                                    
 6   0   100 100 100 100 100 100 100 0                                    
 7   100 100 100 100 100 100 100 100 0                                    
 8   100 100 100 100 100 100 100 100 0                                    
 9   100 100 100 100 100 100 100 100 0                                    
10   0*  100 50  100 100 100 100 100 0                                    
11   0   100 95  100 100 100 100 100 0                                    
12   0   0*  0*  100 100 100 100 95  0                                    
13   50  100 100 100 100 100 100 100 0                                    
14   0   100 50  75  100 0   90  0   0                                    
15   0   100 0*  100 100 100 100 100 0                                    
16   0   0*  0*  0   100 100 100 100 0                                    
17   75  100 50  100 100 100 100 75  0                                    
18   50  100 100 100 100 100 100 100 0                                    
19   00  100 100 100 100 100 100 100 0                                    
20   0   100 100 100 100 100 100 100 0                                    
21   0   0*  0*  100 100 100 100 100 0                                    
22   100 100 100 100 100 100 100 100 0                                    
23   100 100 100 .   100 100 100 100 0                                    
24   0   75  100 .   100 100 100 100 0                                    
27   75  100 100 .   0   100 100 100 0                                    
28   100 100 100 100 100 100 100 100 50                                   
29   100 100 100 100 100 100 100 100 0                                    
30   100 100 100 100 100 100 90  75  0                                    
__________________________________________________________________________
 *Phytotoxic                                                              
 
    
     
                                           TABLE IVb                               
__________________________________________________________________________
IN-VITRO PLANT FUNGICIDE DATA                                             
% Control at (ppm) indicated                                              
Cpd #                                                                     
    ALT                                                                   
       BOC                                                                
          CER                                                             
             COL                                                          
                FUS                                                       
                   HEL                                                    
                      MON PHY                                             
                             PIR                                          
                                PSH                                       
                                   PYU                                    
                                      RHI                                 
                                         SEP                              
                                            UST                           
                                               XAN                        
__________________________________________________________________________
(25)                                                                      
    100                                                                   
       100                                                                
          100                                                             
             95 100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            100                           
                                               0                          
(12)                                                                      
    100                                                                   
       100                                                                
          100                                                             
             95 100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            100                           
                                               0                          
(6) 50 0  100                                                             
             0* 100                                                       
                   75 100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            100                           
                                               0                          
(3) 50 0  95 0  75 50 95  100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         0* 100                           
                                               0                          
(1.5)                                                                     
    0  0  0  0  0* 0* 75  50 50 100                                       
                                   100                                    
                                      100                                 
                                         0  100                           
                                               0                          
(.8)                                                                      
    0  0  0  0  0* 0* 0   0  0  75 0  100                                 
                                         0  50 0                          
(.4)                                                                      
    0  0  0  0  0* 0  0   0  0  0  0  75 0  0  0                          
(.2)                                                                      
    0  0  0  0  0* 0  0   0  0  0  0  0  0  0  0                          
19                                                                        
(25)                                                                      
    100                                                                   
       90 100                                                             
             0  0* 100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            100                           
                                               0                          
(12)                                                                      
    100                                                                   
       50 50 0  0  0  100 100                                             
                             90 100                                       
                                   100                                    
                                      50 100                              
                                            100                           
                                               0                          
(6) 75 0  50 0  0  0  100 0  0  100                                       
                                   0  0  100                              
                                            100                           
                                               0                          
(3) 50 0  0  0  0  0  75  0  0  75 0  0  95 0  0                          
(1.5)                                                                     
    0  0  0  0  0  0  50  0  0  50 0  0  50 0  0                          
(.8)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  0                          
(.4)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  0                          
(.2)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  0                          
28                                                                        
(25)                                                                      
    100                                                                   
       100                                                                
          100                                                             
             50 100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            100                           
                                               --                         
(12)                                                                      
    100                                                                   
       50 100                                                             
             0  100                                                       
                   100                                                    
                      100 100*                                            
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         50 100                           
                                               --                         
(6) 75 0  100                                                             
             0  0  100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         0  100                           
                                               --                         
(3) 0* 0  0  0  0  75 95  100                                             
                             50 100                                       
                                   95 100                                 
                                         0  100                           
                                               --                         
(1.5)                                                                     
    0* 0  0  0  0  0* 0   0  0  95 0  100                                 
                                         0  50 --                         
(.8)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  --                         
(.4)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  --                         
(.2)                                                                      
    0  0  0  0  0  0  0   0  0  0  0  0  0  0  --                         
30                                                                        
(25)                                                                      
    100                                                                   
       90 100                                                             
             100                                                          
                100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            0                             
(12)                                                                      
    100                                                                   
       0  100                                                             
             100                                                          
                100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            0                             
(6) 90 0  50 50 100                                                       
                   100                                                    
                      100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      100                                 
                                         100                              
                                            0                             
(3) 50 0  0  0  100                                                       
                   50 100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      50 100                              
                                            0                             
(1.5)                                                                     
    0  0  0  0  0  0* 100 100                                             
                             100                                          
                                100                                       
                                   100                                    
                                      0  100                              
                                            0                             
(.8)                                                                      
    0  0  0  0  0  0* 100 0  100                                          
                                100                                       
                                   100                                    
                                      0  100                              
                                            0                             
(.4)                                                                      
    0  0  0  0  0  0  100 0  0  100                                       
                                   100                                    
                                      0  0  0                             
(.2)                                                                      
    0  0  0  0  0  0  100 0  0  0  100                                    
                                      0  0  0                             
__________________________________________________________________________
 
    
     
                                           TABLE V                                 
__________________________________________________________________________
BIOCIDES BROAD SPECTRUM SECONDARY TEST RESULTS                            
AGAINST 7 FUNGI                                                           
Cpd. #                                                                    
    PPM ANIG                                                              
            PFUN CRES                                                     
                     APUL GTRA                                            
                              SSER RRUB                                   
__________________________________________________________________________
 1  25  1   1    1   1    1   1    1                                      
    5   1   0    0   0    1   0    0                                      
    1   0   0    0   0    1   0    0                                      
    0.2 0   0    0   0    0   0    0                                      
29  25  1   1    1   1    1   1    1                                      
    5   1   1    1   1    1   1    1                                      
    1   0   0    0   0    1   0    0                                      
    0.2 0   0    0   0    1   0    0                                      
__________________________________________________________________________
 .sup.1 Test was run on Mycophile agar, pH 5.0                            
 1 = no growth                                                            
 0 = growth of the indicated fungi at dose indicated in ppm.              
 
    
     
                                           TABLE VI                                
__________________________________________________________________________
BIOCIDES SECONDARY TEST RESULTS AGAINST 8 FUNGI                           
(Tested on Mycophile Agar at pH 5.0 at indicated dose level)              
Cpd. #                                                                    
    PPM                                                                   
       ANIG                                                               
           PFUN                                                           
               CRES                                                       
                   APUL                                                   
                       CGLO                                               
                           GTRA                                           
                               SSER                                       
                                   RRUB                                   
__________________________________________________________________________
28  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       0   0   0   0   0   0   0   0                                      
    1.0                                                                   
       0   0   0   0   0   0   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
29  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       1   1   0   0   0   1   0   0                                      
    1.0                                                                   
       1   0   0   0   0   1   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
30  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       0   0   0   0   1   1   1   0                                      
    1.0                                                                   
       0   0   0   0   0   0   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
31  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       1   1   0   0   1   1   0   0                                      
    1.0                                                                   
       0   0   0   0   0   0   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
32  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       0   0   0   0   0   0   0   0                                      
    1.0                                                                   
       0   0   0   0   0   0   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
34  25 1   1   1   1   1   1   1   1                                      
    2.5                                                                   
       1   1   0   0   0   1   0   0                                      
    1.0                                                                   
       1   0   0   0   1   1   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
35  25 1   1   1   1   1   1   1   1                                      
    2.1                                                                   
       1   1   0   0   1   1   0   1                                      
    1.0                                                                   
       1   0   0   0   1   1   0   0                                      
    0.2                                                                   
       0   0   0   0   0   0   0   0                                      
__________________________________________________________________________
 
    
     
                       TABLE VII                                                   
______________________________________                                    
MIC TEST AGAINST THREE FUNGI                                              
(Test was in Mycophile Broth, pH 5.0, serial dilutions with a             
starting concentration of 50 ppm, test was read after 8 days of           
incubation.)                                                              
Cpd. #      ANIG       APUL       GTRA                                    
______________________________________                                    
 1          3.2        12.5       1.6                                     
 2          3.2        25         1.6                                     
 3          25         50         3.2                                     
 4          50         50         6.2                                     
 5          &gt;50        &gt;50        6.2                                     
 6          12.5       25         3.2                                     
 7          25         50         12.5                                    
 8          12.5       25         12.5                                    
 9          25         50         12.5                                    
10          6.2        12.5       6.2                                     
11          25         50         12.5                                    
12          25         &gt;50        6.2                                     
13          25         50         6.2                                     
14          50         &gt;50        12.5                                    
15          &gt;50        &gt;50        50                                      
16          &gt;50        &gt;50        25                                      
17          12.5       50         25                                      
18          12.5       50         12.5                                    
19          50         50         50                                      
20          50         50         12.5                                    
21          50         &gt;50        50                                      
22          12.5       50         12.5                                    
23          6.2        25         1.6                                     
24          12.5       25         12.5                                    
25          50         &gt;50        6.2                                     
26          25         50         6.2                                     
27          25         50         6.2                                     
28          12.5       50         6.2                                     
29          1.6        12.5       3.2                                     
30          12.5       50         6.2                                     
31          1.6        6.2        0.8                                     
32          12.5       25         3.2                                     
34          1.6        12.5       3.2                                     
35          1.6        6.2        0.4                                     
______________________________________                                    
 
    
     
                       TABLE VIII                                                  
______________________________________                                    
BIOCIDES MIC TEST AGAINST MIXED COOLING                                   
TOWER ALGAE                                                               
Cpd. #                PPM                                                 
______________________________________                                    
28                    6.2                                                 
29                    6.2                                                 
30                    6.2                                                 
31                    6.2                                                 
32                    6.2                                                 
34                    12.5                                                
35                    6.2                                                 
______________________________________                                    
 
    
     
                       TABLE IX                                                    
______________________________________                                    
MICROORGANISMS USED IN THE BIOCIDES TESTS                                 
                              Abbreviation                                
Name            GRAM ATCC No. used                                        
______________________________________                                    
BACTERIA                                                                  
 1. Proteus mirabilis                                                     
                    (-)      9921   PMIR                                  
 2. Pseudomonas aeruginosa                                                
                    (-)     15442   PSAE                                  
 3. Streptomyces spp.                                                     
                    (+)     19930   STRP                                  
 4. Staphylococcus aureus                                                 
                    (+)      6538   SAUR                                  
 5. Escherichia coli                                                      
                    (-)     11229   ECOL                                  
 6. Pseudomonas fluorescens                                               
                    (-)      948    PSFL                                  
 7. Serratia marescens                                                    
                    (-)       60    SMAR                                  
 8. Enterobacter aerogens                                                 
                    (-)     13048   EEAE                                  
 9. Bacillus subtilis                                                     
                    (+)      6633   BSUB                                  
10. Pseudomonas cepacia                                                   
                    (-)     17765   PSCP                                  
11. Salmonella choleraesuis                                               
                    (-)     10708   SCHO                                  
12. Flavobacterium flavescens                                             
                    (-)      8315   FFLA                                  
FUNGI                                                                     
 1. Aspergillus niger    6275     ANIG                                    
 2. Penicillium funiculosum                                               
                        11797     PFUN                                    
 3. Cladosporium resinae                                                  
                        52833     CRES                                    
 4. Aureobasidium pullulans                                               
                         9348     APUL                                    
 5. Chaetomium globosum  6205     CGLO                                    
 6. Poria placenta      11538     PPLA                                    
 7. Gloeophyllum trabeum                                                  
                        11539     GTRA                                    
YEAST                                                                     
 8. Sacharomyces cerevisiae                                               
                         560      SSER                                    
 9. Rhodotorula rubra    2503     RRUB                                    
______________________________________