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spec: Major constraints on indigenous NGOs have roots in underlying power structures that value technocratic and operational skills common among Northern NGOs , over the kinds of skills that local NGOs may have to offer . In the case of previously undescribed toxins from these isolates , active compounds will be isolated by bioassay-guided fractionation ( using the zebrafish embryo assay ) , and chemically characterized . Why should you care ? We therefore asked whether we could detect H-Ras mutations in mammary tumors induced by an MMTV-Wnt1 transgene in a p53-null background ; a failure to find such mutations would imply either that p53 is somehow required for such mutations to occur or , more likely , that an inherited deficiency of p53 conferred a growth advantage to Wnt1-expressing mammary cells that would outweigh any selective advantage provided by a secondary somatic mutation in H-Ras . " The skillful hearer will ask many questions , and the well-trained child will question , too . This risk is unjustifiable when significant improvements can be made in the current environment . He was apparently animated by a calmly philosophic resignation , as if the removal were the merest transient incident which would cause the least trouble if facilitated and disposed of once and for all . This includes informing students , prospective students , and others about educational opportunities and admission procedures , providing academic and career advising , facilitating transfer , serving students with disabilities , and supporting the work of the student affairs staffs on such student life issues as alcohol and other drug abuse . Who is right ? You will also see detailed airport and airspace information .