the study , which was conducted after the required permit is obtained from the local council of ethics for animal experiments in sleyman demirel university , was conducted in the experiment animals and medical investigation implementation and research center in sleyman demirel university . 32 adult male sprague - dawley rats were divided into the following four groups , each containing eight rats : control ( c ) group , streptozotocin ( stz ) group , viscum album ( va ) group and viscum album + streptozotocin ( va+stz ) group . after the stz had been prepared in citrated buffer ( 0.1 m , ph 4.5 ) with a rate of 55 mg / kg , it was applied to rats in a single dose and in an intraperitoneal way . depending on their groups , the prepared extract was given to the rats daily by means of gavage for a period of 10 days ( 100 mg / kg ) . on the 11 day , diabetes was induced by means of streptozotocin ( 55 mg / kg ) in specified groups . the values were measured by means of the blood taken from their tails to glucometer strips . first , the leaves of the viscum album collected from the pnargz region of the elmalyurt village in the district of glhisar in the province of burdur on january and february were separated and were dried in 45c temperature for a period of 5 - 6 hours by the researcers . the dry and ground leaves were mixed with the 95 % ethanol at a rate of 1:5 . then the mixture was kept at 4c for a period of 24 hours before sieving it using a 0.45 m membrane filter . after ethanol is removed from the solution , it was kept at -20c until it is used . the viscum album extract was given to the rats in the relevant groups ( to the groups  va and  va+stz ) by means of oral gavage at a dose of 100 mg / kg for a period of 10 days . after the stz had been prepared in citrated buffer ( 0.1 m , ph 4.5 ) with a rate of 55 mg / kg , it was applied to rats in a single dose and in an intraperitoneal way . the plasma glucose levels were determined by measuring them by glucometer using a drop of blood taken from the tip of the tails of the rats . accutrend plus gct glucometer was used for measuring of the plasma glucose levels of rats . the monitor of the glucometer device uses a reflectance photometer for measuring the intensity of the color formed at the end of the reaction . bis- next mediator in oxidized form enzyme returning to step ( 2-hydroxyethyl ) - ( 4-hidroksiiminohekzo from 2,5-dienyl ) ammonium chloride selectively reduces . it was assumed that the disease had developed if the blood glycose level exceeds 250 mg / dl . after the stz had been applied , the blood glycose levels were monitored on a daily basis . at the end of the two weeks , the rats were sacrificed , their liver and kidney tissues were removed and their antioxidant parameters were examined . 0.05 m tris - hcl buffer with a ph value of 7.4 was used in the homogenization of the tissues . the tissue samples were weighed out by using a sensitive balance and their wet weights were recorded . then they were placed in eppendorf tubes and kept at -80c until the biochemical examinations are conducted on them . mda - cat and serum glucose levels in groups ( meansem ) c : control group ; stz : streptozotocin group ; va : viscum album group and viscum album+streptozotocin ( va+stz ) group ; sem : standard error mean . at the beginning of the homogenization process , the wet weights of the tissues were determined . they were put into glass tubes by maintaining their low temperature and then 2 ml of cold tris - hcl buffer was added over them . then plastic bowls were filled with ice and the tissues in glass tubes were placed in these plastic bowls and they were homogenized at 16.000 rev / min for a period of 3 minutes . buffer was added in a way that the last volume was 10 times the weight of the weight of the tissue . by maintaining them in low temperature , the amount of mda protein was determined by using the homogenates obtained . by means of the centrifugation of the homogenates at + 4c at 5.000 rev ( min for a period of 30 minutes , then , from the selected supernatants , the amount of proteins was determined by means of cat enzyme activities . determination of the proteins in the homogenates , supernatants and extracts of the homogenized samples was carried out by using the lowry method . according to this method , the cupper - protein complex is formed in the alkali solution , which , in turn , reduces the folin - ciocalteu - phenol reagent , resulting in a dark blue color . the degree of the darkness of the blue color is directly proportional with the concentration of the protein . the amount of malondialdehyde was determined by the spectrophotometric method based on the draper and hadley s method . in this method , the color produced by the reaction of mda and thiobarbituric acid ( tba ) is considered by means of a spectrophotometric measurement . the h2o2 added into the experimental environment is broken down by the catalase enzyme into water and oxygen , which manifests itself on the ultraviolet spectrum as a decrease in the absorbance . this decrease in the absorbance is directly proportional to the activity of the cat enzyme . anova which is a parametric test , was performed for comparing of groups mda and cat levels . 32 adult male sprague - dawley rats weighing 200 - 250 g were used for experimental procedures . the ambient temperature and relative humidity of the animal room were 211c and 607% , respectively . the study , which was conducted after the required permit is obtained from the local council of ethics for animal experiments in sleyman demirel university , was conducted in the experiment animals and medical investigation implementation and research center in sleyman demirel university . 32 adult male sprague - dawley rats were divided into the following four groups , each containing eight rats : control ( c ) group , streptozotocin ( stz ) group , viscum album ( va ) group and viscum album + streptozotocin ( va+stz ) group . after the stz had been prepared in citrated buffer ( 0.1 m , ph 4.5 ) with a rate of 55 mg / kg , it was applied to rats in a single dose and in an intraperitoneal way . depending on their groups , the prepared extract was given to the rats daily by means of gavage for a period of 10 days ( 100 mg / kg ) . on the 11 day , diabetes was induced by means of streptozotocin ( 55 mg / kg ) in specified groups . the values were measured by means of the blood taken from their tails to glucometer strips . first , the leaves of the viscum album collected from the pnargz region of the elmalyurt village in the district of glhisar in the province of burdur on january and february were separated and were dried in 45c temperature for a period of 5 - 6 hours by the researcers . the dry and ground leaves were mixed with the 95 % ethanol at a rate of 1:5 . then the mixture was kept at 4c for a period of 24 hours before sieving it using a 0.45 m membrane filter . after ethanol is removed from the solution , it was kept at -20c until it is used . the viscum album extract was given to the rats in the relevant groups ( to the groups va and  va+stz ) by means of oral gavage at a dose of 100 mg / kg for a period of 10 days . after the stz had been prepared in citrated buffer ( 0.1 m , ph 4.5 ) with a rate of 55 mg / kg , it was applied to rats in a single dose and in an intraperitoneal way . the plasma glucose levels were determined by measuring them by glucometer using a drop of blood taken from the tip of the tails of the rats . accutrend plus gct glucometer was used for measuring of the plasma glucose levels of rats . the monitor of the glucometer device uses a reflectance photometer for measuring the intensity of the color formed at the end of the reaction . bis- next mediator in oxidized form enzyme returning to step ( 2-hydroxyethyl ) - ( 4-hidroksiiminohekzo from 2,5-dienyl ) ammonium chloride selectively reduces . it was assumed that the disease had developed if the blood glycose level exceeds 250 mg / dl . after the stz had been applied , the blood glycose levels were monitored on a daily basis . at the end of the two weeks , the rats were sacrificed , their liver and kidney tissues were removed and their antioxidant parameters were examined . 0.05 m tris - hcl buffer with a ph value of 7.4 was used in the homogenization of the tissues . the tissue samples were weighed out by using a sensitive balance and their wet weights were recorded . then they were placed in eppendorf tubes and kept at -80c until the biochemical examinations are conducted on them . mda - cat and serum glucose levels in groups ( meansem ) c : control group ; stz : streptozotocin group ; va : viscum album group and viscum album+streptozotocin ( va+stz ) group ; sem : standard error mean . at the beginning of the homogenization process , the wet weights of the tissues were determined . they were put into glass tubes by maintaining their low temperature and then 2 ml of cold tris - hcl buffer was added over them . then plastic bowls were filled with ice and the tissues in glass tubes were placed in these plastic bowls and they were homogenized at 16.000 rev / min for a period of 3 minutes . buffer was added in a way that the last volume was 10 times the weight of the weight of the tissue . by maintaining them in low temperature the amount of mda protein was determined by using the homogenates obtained . by means of the centrifugation of the homogenates at + 4c at 5.000 then , from the selected supernatants , the amount of proteins was determined by means of cat enzyme activities . determination of the proteins in the homogenates , supernatants and extracts of the homogenized samples was carried out by using the lowry method . according to this method , the cupper - protein complex is formed in the alkali solution , which , in turn , reduces the folin - ciocalteu - phenol reagent , resulting in a dark blue color . the degree of the darkness of the blue color is directly proportional with the concentration of the protein . the amount of malondialdehyde was determined by the spectrophotometric method based on the draper and hadley s method . in this method , the color produced by the reaction of mda and thiobarbituric acid ( tba ) is considered by means of a spectrophotometric measurement . the h2o2 added into the experimental environment is broken down by the catalase enzyme into water and oxygen , which manifests itself on the ultraviolet spectrum as a decrease in the absorbance . this decrease in the absorbance is directly proportional to the activity of the cat enzyme . spss 20.00 package program was used for statistical analysis . anova which is a parametric test , when the liver mda values are compared between stz and va groups and between stz and c groups , it was seen that the mda values are lower in the stz group ( p=0.006 and 0.015 , respectively ) . the differences between the liver mda values of the other groups were found to be insignificant . the comparison of the kidney mda values between the stz and c groups , stz and va groups , and stz and va+stz groups showed that the mda level was significantly higher in the stz group ( p=0.029 , p=0.014 and p=0.011 , respectively ) . when the kidney cat activity is compared between the stz and c groups and the stz and stz+va groups , it was found to be lower in the stz group ( p=0.028 and p=0.006 ) . when the liver cat values are compared between the stz and c groups and the stz and va groups , a significant decrease was observed in the stz group ( p=0.012 and p=0.006 ) . when the glucose values following the application of stz were examined , it was seen that the glucose value for the va group was higher than that for the c group ( p=0.001 ) ; the glucose value for the stz group was significantly higher than that for the c group ( p=0.000 ) ; and the glucose value for the stz+va group was higher than that for the c group ( p=0.000 ) . when the glucose values for the va and stz groups were compared , it was seen that the glucose value for the stz group was higher ( p=0.000 ) ; and , when the values for the va and stz+va groups were compared , the glucose value for stz+va was found to be higher ( p=0.000 ) . no significant difference was found out between the stz+va and stz groups in terms of their glucose levels . when the balance between the free radicals produced during the physiological processes occurring in the body or during pathological processes and the antioxidant system is disturbed in favor of the free radicals , the oxidative damage occurs . the organism tries to protect itself against this oxidative damage by enzymatic and non - enzymatic ways . catalase and mda are among the important enzymes used in this enzymatic process of protection . diabetes is a disease that affects the millions of people every sex and race effective and globally every year [ 17 , 18 ] . diabetes is a disease which disturbs the glycemic control and the antioxidant metabolism disorder plays a role in the development of the clinic state . in a study investigating the possible effects of viscum album , in decreasing oxidative stress , which have been applied on the stomach and colon tissues of cancer patients and healthy individuals , it has been shown that viscum album has an inhibiting effect on xanthine oxidase and adenosine deaminase . there is also a study which have shown that viscum album might increase the insulin secretion and improve the glycemic control . in this study , rats in which diabetes had been induced by means of stz were given viscum album for short and long periods ( for 4 hours and 3 weeks respectively ) and the antidiabetic and antilipidemic effects of it were examined . the results of this study showed that viscum album had caused hypoglycemia in these rats . in another study carried out on wistar rats , the rats were divided into groups and , following a 12-hour period of hunger , they were loaded , depending on their groups , with glucose and viscum album extract . then blood samples were taken from the rats in order to examine their glycose , insulin and glucagon levels . while the extract resulted in no changes in the glucose levels in healthy rats , it lowered the level of blood sugar in diabetic rats . it was found out that the insulin secretion had increased both in diabetic and healthy rats and it was shown that viscum album could have antiglycemic and insulinotropic effects . in a study carried out on rats in which diabetes had been induced by means of stz and which had been divided into groups based on the amount of viscum album given to them , it was shown that viscum album could have antihyperlipidemic and antioxidant properties ( by releasing mda and gsh ) . in another study carried out at the cellular level , it was shown that the viscum album extract applied to pancreatic b cells could induce the secretion of insulin and thus could be used as an antidiabetic substance . in a study in which the effectiveness of the herbal treatments used for diabetes was investigated , the rats in which diabetes had been induced by means of stz were given viscum album and it was found out that the extract resulted in a decrease in polyphagia and polydipsia . this suggests that viscum album can be used in decreasing the symptoms without having any effect on glycemic control . in the present study , which has been conducted at the biochemical level , the antioxidant activity in the liver and kidney tissues of rats has been investigated . this activity has been tried to be calculated based on the levels of mda , which plays an important role in lipid peroxidation and has a function of decreasing the oxidative stress , and catalase , which is an antioxidant enzyme . when the mda values are compared among the groups formed within the scope of the experiment , significant differences were found out between the stz and va groups ( p=0.014 ) and stz and va+stz groups ( p=0.011 ) , the values being higher for the stz group . in the rats in which diabetes had been induced , the viscum album given beforehand resulted in significant changes in the kidney mda values , which has an effect of decreasing the oxidative stress . when the blood sugar values were analyzed after the application of the stz , no significant difference was found out between the stz+va and stz groups . this indicates that the viscum album given beforehand has no effect on the glycemic control . consequently , an increase in the enzymatic activity of catalase , which is an antioxidant enzyme functioning in the kidney tissue , and a decrease in the level of mda , which is an indication of lipid peroxidation , were observed among the rats given viscum album . considering the fact that a decrease in the oxidative stress provides positive contributions for the improvement of the clinical state and the overall situation in diabetes , it can be said that viscum album can produce benefits in the improvement of the clinical state in diabetics . however , there is a need for further studies , especially the studies investigating the active ingredient , in order to find out the degree of the antioxidant and antidiabetic effects of viscum album .