dermatophytes are responsible for a variety of clinical manifestations in man , trichophyton rubrum being the commonest isolate worldwide . they are assuming greater significance both in developed and developing countries due to illnesses like diabetes , hiv infection and the use of immunosuppressive drugs . overall ringworm infections account for 10% of the total cases who attend the skin outpatient department . the indian subcontinent has a varied topography and the hot and humid climate which is more conducive to the acquisition of fungal infections . the nature of dermatophytosis may change with the passage of time , living conditions and evolution of preventive measures and hygienic conditions in society . the study was conducted in the department of microbiology and dermatovenerology at igmc shimla over a period of one year from may 2008 to april 2009 . the study group comprised of hundred clinically suspected cases of dermatophytosis . a detailed clinical history including age , sex , duration and the clinical presentation were recorded . as per the involvement of the anatomical site they were grouped into various clinical types . direct microscopy using 10% potassium hydroxide ( koh ) for skin scrapings and epilated hairs and 40% koh for nail specimens was done . the koh wet mounts were screened under low power ( 10 ) and then at high power ( 40 ) for visualization of the fungal hyphae . septate hyphae with or without branching and a diameter of 2 - 4 m , or hyphae with arthroconidia were taken as a criterion to label the slide as koh positive . the second part of the sample was inoculated on two sets of sabourauds dextrose agar containing chloramphenicol ( 0.05 mg / ml ) and cyclohexamide ( 0.1 - 0.4 mg / ml ) and incubated at 25 and 37c . the cultures were examined twice a week and if no growth was obtained till 4 weeks they were declared negative . the culture isolates were further identified by studying the colony morphology and microscopic examination of the lactophenol cotton blue mounts . further special tests like hair perforation , urease production and slide culture were performed wherever necessary according to standard techniques . peak incidence of dermatophytosis was seen in the third decade of life ( 28% ) . out of 100 cases 77 ( 77% ) presented with a single site involvement while 23 ( 23% ) had affliction of two or more anatomical sites . tinea corporis was the commonest clinical presentation ( 27.7% ) followed by tinea cruris ( 22% ) . association of tinea corporis with other clinical types was present in 12 out of 23 ( 52.17% ) cases . combination of tinea unguium with tinea pedis and tinea mannum was seen in 11 ( 47.8% ) and 4 ( 17.3% ) out of 23 cases respectively . on koh wet mount examination 80 out of 100 ( 80% ) out of these only 57 samples was culture positive . there were 11 koh negative samples that yielded growth on culture . overall , 68% samples were culture positive , taking into consideration both koh positive and negative specimens [ table 1 ] . correlation between clinical type , koh and culture culture positivity was highest in tinea corporis ( 80.95% ) and lowest in cases of tinea barbae ( 33.33% ) as depicted in table 2 . trichophyton was the most common genus in 66 out of 68 ( 97.05% ) culture isolates . trichophyton rubrum was the commonest species ( 66.17% ) followed by trichophyton mentagrophytes ( 19.11% ) , trichophyton violaceum ( 7.35% ) , trichophyton verrucosum ( 2.94% ) . there was one isolate each of trichopyton tonsurans ( 1.47% ) and microporum gypseum respectively [ table 3 ] . maximum number of cases were seen in the age group of 21 - 30 years ( 28% ) , followed by 31 - 40 years ( 24% ) and males were affected more than females . it can be attributed to the fact that the males may be more prone because of prolonged vigorous outdoor activity and increased perspiration which creates an environment for the development of tinea infection . in the present study tinea corporis this pattern of clinical preponderance has been observed in other studies . on the contrary reports from manipal and aurangabad perhaps these areas are hot and humid as compared to our region which has a temperate climate which accounts for the difference . some reports quote similar rates however others have reported a larger proportion of cases with this clinical type . this could be attributed to the difference in the personal hygiene and habits of the people . wet mount examination positivity rates ranging from 23.8% to as high as 91.2% have been reported . in our study selection criteria of cases and skill involved in sampling techniques perhaps accounts for the difference . thus all koh negative samples should be cultured . in a study conducted in ludhiana 13.2% of koh negative samples exhibited growth on culture which coincides with our observations . non visualization of hyphae on direct microscopy could possibly be due to a severe inflammatory reaction which obscures them . a variability in culture isolation ranging from 44.6% to70.7% has been found in the indian sub - continent . in this anthropophillic species has become well adapted to the human skin as climatic and crowded living conditions enhance its prevalence . trichophyton violaceum was isolated from cases of tinea capitis in children in the prepubertal age group . however in a study conducted in hyderabad higher isolation rates upto 14.68% have been observed . this could be attributed to the predominant muslim population who wear caps and thus are more prone to the acquisition of superficial fungal infections . one isolate each of trichophyton verrucosum and microsporum gypseum were obtained from cases of tinea facei . both these species were isolated from children with a rural background so a probable transmission of infection could have occurred from above sources . isolation of microsporum gypseum from one case is of special interest in the present study as this genus is generally less commonly encountered in india . one isolate each of trichophyton violaceum and trichopyton tonsurans were recovered from nail specimens on culture . the present study has given us a clear insight regarding the mycological pattern of dermatophytosis in this region . the overall findings suggest that the study is comparable to those from different parts of india .