an independent clinical research organization monitored the study progress and results , and the study was registered through clinicaltrials.gov , identifier nct02330523 . forty subjects , 1870  years of age , intended for extraction and subsequent implant placement , were enrolled with 10 study sites . only posterior ( first premolar to first molar ) extraction sockets , with intact adjacent teeth or dental implants ( for measurement stent indexing ) and with substantial buccal wall dehiscences of at least 1/3 the overall socket height and width were included ( class ii / iii , elian et  al . subjects were excluded if they had a history of tobacco use within the last 6  months , healing disorders , i.e. diabetes mellitus , cancer , hiv , bone metabolic diseases , or had received systemic corticosteroids , immunosuppressive agents , radiation therapy , and/or chemotherapy within the past 2  months . subjects taking intramuscular or intravenous bisphosphonates or who had allergies or sensitivity to alginate , latex , collagen or acrylic were also excluded , as were pregnant , lactating or intending to become pregnant women , or those participating in other clinical intervention studies . the ridge preservation control therapy was demineralized allograft ( oragraft dgc , lifenet health , inc . , virginia beach , va , usa ) plus reconstituted and crosslinked bovine collagen membrane ( biomend extend ; zimmer dental , inc . , the ridge preservation test therapy was deproteinized bovine bone mineral with collagen binder plus extracted , native porcine , bilayer collagen membrane ( biooss collagen plus biogide ; geistlich pharma ag , wolhusen , switzerland ) subjects were randomly assigned to either the control or test therapy in a block 1:1 ratio , first by the 10 investigators and then overall for all 40 subjects , so that each centre might have equal numbers of test and control subjects , with a minimum of one and a maximum of three test and control patients per investigator . investigators received randomization instructions only after enrolling a  subject and immediately prior to surgery . primary and secondary endpoints and exploratory measures were as follows : 
 primary outcome variable 
 bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . secondary outcome variables 
 bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . exploratory variables 
 histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
 wound closure ( incision line gap ) at all time points.soft tissue inflammationbaseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . 


  primary outcome variable 
 bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . secondary outcome variables 
 bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . 
  bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . exploratory variables 
 histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
 wound closure ( incision line gap ) at all time points.soft tissue inflammationbaseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
  % new bone in contact with graft % graft in contact with new bone wound closure ( incision line gap ) at all time points . soft tissue inflammation baseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket . bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . prior to surgery , alginate impressions were obtained for fabrication of indexed measuring stents ( see fig medical and dental histories and demographic data were recorded ; oral exams and dental cleanings were performed . ( a ) baseline extraction socket measures insured that buccal wall mesialdistal and vertical dehiscences were at least 1/3 of the overall extraction socket dimensions . ( b & c ) measuring stents were fabricated from 0.020  thermoplastic and registered on adjacent teeth . the stents included three indexing holes for measuring ridge buccallingual width , vertical height to the lingual wall and vertical height to the buccal wall . following administration of antibiotics , extractions were performed with flap reflection , periotomes , elevators and forceps . to ensure that appropriate biopsy specimens were obtained , i.e. new bone and graft and not old host bone in the cores , extraction socket morphology dimensions were recorded through direct measures , and baseline extraction socket buccallingual and vertical dimensions were recorded using indexed stents , as described above and shown in fig . co. , chicago , il , usa ) , depending on the dimension of the defect . the measurement methods were similar to those employed by wood and mealey ( wood & mealey 2012 ) . bone defects were filled with particulate bone grafts confined to the existing alveolar ridge dimensions , making no attempt to go outside the confines of ridge , and then covered with absorbable collagen membranes placed to cover the grafts and extend slightly 23  mm beyond bone defect margins . periosteal releasing incisions were not employed , and incision lines were not required to be closed primarily so that mucogingival junction dimensions might be preserved . remaining soft tissue incision gaps , if any , were recorded both mesiodistally and buccallingually . subjects were instructed to take ibuprofen 800  mg or hydrocodone / paracetamol up to three times a day for pain , as needed . subjects were instructed to use chlorhexidine ( 0.12% ) mouth rinse for 30  s twice daily and to avoid excessive muscle tractioning or trauma and not to brush the study areas for the first 2  weeks . amoxicillin was provided 875  mg bid for 1014  days ( with clindamycin 300  mg qid for 710  days for subjects with penicillin allergies ) . after 2  weeks of healing , subjects were instructed in a brushing technique creating minimal apically directed trauma to the treatment area . at 4  weeks , the subjects were instructed to stop chlorhexidine rinses and resume normal oral hygiene practices . at 1week postsurgery , photos of the test sites were taken , and clinical measurements of inflammation and remaining incision line gaps ( if any ) were assessed . categorical inflammation scores were : 

 0  normal , ( absence of inflammation ) 
 1   mild inflammation of any portion of the marginal unit , e.g. , slight change of colour  
 2  mild inflammation of entire gingival unit ( but no oedema ) 
 3  moderate inflammation ( moderate glazing , redness , oedema and/or hypertrophy ) 
 4  severe inflammation ( marked redness and oedema / hypertrophy , spontaneous bleeding or ulceration ) 
 
 0  normal , ( absence of inflammation ) 
 1   mild inflammation of any portion of the marginal unit , e.g. , slight change of colour  
 2  mild inflammation of entire gingival unit ( but no oedema ) 
 3  moderate inflammation ( moderate glazing , redness , oedema and/or hypertrophy ) 
 4  severe inflammation ( marked redness and oedema / hypertrophy , spontaneous bleeding or ulceration ) further followup evaluations occurred at 4weeks , 3months and 6months postsurgery . photos of the test sites and clinical measurements of remaining incision line gaps ( if any ) were obtained , and inflammation was assessed . oral hygiene instructions were reviewed throughout the study . at 1 , 3 and 6  months , to implant placement , vertical and horizontal ridge dimensions were recorded using the indexed measuring stents . to retrieve biopsies approximately 810  mm in length , 3  mm trephines ( ace surgical supply , bur trephine 35 tl 2id 2.8od ti 18.5cl ca marks 810131518 ) were used . biopsies were retrieved from the implant sites , unless an implant was placed in original , nongrafted bone , in which case the biopsy was retrieved from the grafted area immediately adjacent to the implant , as indicated by the indexed stent . biopsies were retained within their respective trephines , wrapped with lintfree gauze ( the open ends of the trephines serving as the apical orientation reference ) and preserved in 10% buffered formalin solution . trephines together with biopsies were fixed in 4% formalin for 57  days , dehydrated in serial steps of ethanol ( 70% , 80% , 90% , 100% ) , remaining for 1  day in each concentration , and degreased for 1  day in xylene . specimens were then infiltrated , embedded and polymerized ( technovit 9100heraeus kulzer , wehrheim , germany ) . after polymerization , samples were hemisected and cut in 500  m sections using a precision cutting machine ( secotom 50 , stuers , ballerup , denmark ) , so that two sections were obtained per biopsy . the sections were mounted onto opacified acrylicslides and ground to a final thickness of approximately 60  m on a rotating grinding plate ( stuers , ballerup , denmark ) . specimens were subsequently stained with azure ii and pararosaniline ( axio imager m1 and axiocam hrc , carl zeiss , gttingen , germany ) . histologic evaluation was performed with a microscope equipped with a digital analyzer ( axio imager m1 and axiocam hrc , carl zeiss , gttingen , germany ) . histomorphometric analyses were performed with imaging software ( analysis five  soft imaging system , mnster , germany ) on composite overview scans . the area of new healing ( versus old / original bony tissues ) was demarcated in each section . within this area , prior to initiation of the study , using an indexed stent , stone cast model of an extraction socket exhibiting a substantial buccal dehiscence and a unc 15 periodontal probe ( hufriedy mfg . co. , chicago , il , usa ) , all investigators were calibrated ( 1  mm ) to the principal investigator ( ets ) . the first step in the analysis was to determine if the randomization resulted in balanced groups . age , gender , race , ethnicity , bmi and all baseline clinical variables were tested to determine if the two groups were balanced . the analyses were based on the variable types , with dichotomous variables tested using fisher exact test and categorical variables using chisquare test and ttests for continuous variables . the primary hypothesis was that dbbmc  +  nbcm ( test ) was not inferior to dfdba  +  recxc ( control ) in the preservation of ridge volume as measured by the difference in the horizontal distance from stents , buccolingually to the interior ( lingual ) bony wall . the noninferiority margin was 1.5  mm , as obtained from the approximate deviation in ridge dimension observed by wood and mealey . twosample ttests were used to evaluate noninferiority , such that : 
 h0 :  
 ck     
 ob     
 o ha :  
 ck     
 ob  >   
 o ck  =  the mean change in ridge volume for dbbmc  +  nbcm from baseline to 6  months 
  
 ob  =  the mean change in ridge volume for dfdba  +  recxc from baseline to 6  months 
  
 o  =  hypothesized mean difference  =  0.0  mm 
   =  noninferiority margin  =  1.5  mm 
   =  0.05 , onesided1  =  0.90 
 
  
 ck  =  the mean change in ridge volume for dbbmc  +  nbcm from baseline to 6  months 
  
 ob  =  the mean change in ridge volume for dfdba  +  recxc from baseline to 6  months 
  
 o  =  hypothesized mean difference  =  0.0  mm 
   =  noninferiority margin  =  1.5  mm if the test for noninferiority was statistically significant , then a twosample ttest for superiority was subsequently conducted , as detailed in the methodology of morikawa & yoshida ( 1995 ) . treatment differences in the vertical ridge preservation changes ( baseline to 6  months ) , measured apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls  were also evaluated using the noninferiority method listed above . differences in histomorphometric variables were tested with twosample ttest for superiority due to the exploit nature of the variables at 6  months . due to multiple testing for the five histomorphometric variables , the significance level had to be less  than 0.05/6  =  0.008 ( bonferroni ) . for histomorphometric differences to be clinically significant , a margin of at least 15.0% was needed based on the work of wood and mealey . the behaviour of incision line closure over time was an additional goal of the analysis , with evaluations at 1  week , 1  month and at 3  months . inferiority testing could not be done due to a lack of historical data and the potential time dependence of the data . in addition to mean differences tested with ttests , the frequency of closed incisions was tested at each time point with fisher 's exact test . sample size was predicated on assuring that the primary study objective had adequate power to assess the noninferiority hypothesis . under these assumptions , proc power ( sas version 9.2 , sas institute , inc , cary , north carolina , usa ) required a sample size of 18 evaluable subjects in each treatment group . therefore , a sample size of 40 subjects was considered sufficient to meet the primary objective of this study . although the study was powered for the primary outcome variable , the secondary outcome variables each retained 90% power to demonstrate noninferiority , assuming that the treatments were , in truth , equal subjects were enrolled by assuring verbal understanding and obtaining written informed consents , which , along with the study protocol , were reviewed by an ethical review board in accordance with the declaration of helsinki . an independent clinical research organization monitored the study progress and results , and the study was registered through clinicaltrials.gov , identifier nct02330523 . forty subjects , 1870  years of age , intended for extraction and subsequent implant placement , were enrolled with 10 study sites . only posterior ( first premolar to first molar ) extraction sockets , with intact adjacent teeth or dental implants ( for measurement stent indexing ) and with substantial buccal wall dehiscences of at least 1/3 the overall socket height and width were included ( class ii / iii , elian et  al . subjects were excluded if they had a history of tobacco use within the last 6  months , healing disorders , i.e. diabetes mellitus , cancer , hiv , bone metabolic diseases , or had received systemic corticosteroids , immunosuppressive agents , radiation therapy , and/or chemotherapy within the past 2  months . subjects taking intramuscular or intravenous bisphosphonates or who had allergies or sensitivity to alginate , latex , collagen or acrylic were also excluded , as were pregnant , lactating or intending to become pregnant women , or those participating in other clinical intervention studies . the ridge preservation control therapy was demineralized allograft ( oragraft dgc , lifenet health , inc . , virginia beach , va , usa ) plus reconstituted and crosslinked bovine collagen membrane ( biomend extend ; zimmer dental , inc . , the ridge preservation test therapy was deproteinized bovine bone mineral with collagen binder plus extracted , native porcine , bilayer collagen membrane ( biooss collagen plus biogide ; geistlich pharma ag , wolhusen , switzerland ) subjects were randomly assigned to either the control or test therapy in a block 1:1 ratio , first by the 10 investigators and then overall for all 40 subjects , so that each centre might have equal numbers of test and control subjects , with a minimum of one and a maximum of three test and control patients per investigator . investigators received randomization instructions only after enrolling a  subject and immediately prior to surgery . primary and secondary endpoints and exploratory measures were as follows : 
 primary outcome variable 
 bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . secondary outcome variables 
 bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . exploratory variables 
 histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
 wound closure ( incision line gap ) at all time points.soft tissue inflammationbaseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . 


  primary outcome variable 
 bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . bone ridge dimension preservation horizontal change from baseline to 6  months , as measured using indexed stents , from the outside of the stent , at the apical extent of the buccal dehiscence , buccolingually to the interior bony wall . secondary outcome variables 
 bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . 
  bone ridge dimension preservation vertical changes from baseline to 6  months , apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls . exploratory variables 
 histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
 wound closure ( incision line gap ) at all time points.soft tissue inflammationbaseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . histomorphometric % new bone , % graft and % connective tissue / bone marrow components from midsection bone core biopsies at 6  months , including : 
 % new bone in contact with graft% graft in contact with new bone 
  % new bone in contact with graft % graft in contact with new bone wound closure ( incision line gap ) at all time points . soft tissue inflammation baseline bone measurements  extraction socket morphology measured directly at baseline surgery 
 baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket.bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . 
  baseline extraction socket dimensions , measured as the  mesialdistal distance of the dehiscence at the crest and the coronalapical distance from the crest to most apical extent of the buccal dehiscence , and also as the overall socket dimensions , mesialdistal at crest and coronalapical from crest to the apical extent of the extraction socket . bony wall thickness , measured horizontally at the coronal tip of the most apical extent of the dehiscences of the buccal and , if present , lingual bony walls . prior to surgery , alginate impressions were obtained for fabrication of indexed measuring stents ( see fig . medical and dental histories and demographic data were recorded ; oral exams and dental cleanings were performed . ( a ) baseline extraction socket measures insured that buccal wall mesialdistal and vertical dehiscences were at least 1/3 of the overall extraction socket dimensions . ( b & c ) measuring stents were fabricated from 0.020  thermoplastic and registered on adjacent teeth . the stents included three indexing holes for measuring ridge buccallingual width , vertical height to the lingual wall and vertical height to the buccal wall . following administration of antibiotics , extractions were performed with flap reflection , periotomes , elevators and forceps . to ensure that appropriate biopsy specimens were obtained , i.e. new bone and graft and not old host bone in the cores , extraction socket morphology dimensions were recorded through direct measures , and baseline extraction socket buccallingual and vertical dimensions were recorded using indexed stents , as described above and shown in fig . co. , chicago , il , usa ) , depending on the dimension of the defect . the measurement methods were similar to those employed by wood and mealey ( wood & mealey 2012 ) . bone defects were filled with particulate bone grafts confined to the existing alveolar ridge dimensions , making no attempt to go outside the confines of ridge , and then covered with absorbable collagen membranes placed to cover the grafts and extend slightly 23  mm beyond bone defect margins . periosteal releasing incisions were not employed , and incision lines were not required to be closed primarily so that mucogingival junction dimensions might be preserved . remaining soft tissue incision gaps , if any , were recorded both mesiodistally and buccallingually . subjects were instructed to take ibuprofen 800  mg or hydrocodone / paracetamol up to three times a day for pain , as needed . subjects were instructed to use chlorhexidine ( 0.12% ) mouth rinse for 30  s twice daily and to avoid excessive muscle tractioning or trauma and not to brush the study areas for the first 2  weeks . amoxicillin was provided 875  mg bid for 1014  days ( with clindamycin 300  mg qid for 710  days for subjects with penicillin allergies ) . after 2  weeks of healing , subjects were instructed in a brushing technique creating minimal apically directed trauma to the treatment area . at 4  weeks , the subjects were instructed to stop chlorhexidine rinses and resume normal oral hygiene practices . at 1week postsurgery , photos of the test sites were taken , and clinical measurements of inflammation and remaining incision line gaps ( if any ) were assessed . categorical inflammation scores were : 

 0  normal , ( absence of inflammation ) 
 1   mild inflammation of any portion of the marginal unit , e.g. , slight change of colour  
 2  mild inflammation of entire gingival unit ( but no oedema ) 
 3  moderate inflammation ( moderate glazing , redness , oedema and/or hypertrophy ) 
 4  severe inflammation ( marked redness and oedema / hypertrophy , spontaneous bleeding or ulceration ) 
 
 0  normal , ( absence of inflammation ) 
 1   mild inflammation of any portion of the marginal unit , e.g. , slight change of colour  
 2  mild inflammation of entire gingival unit ( but no oedema ) 
 3  moderate inflammation ( moderate glazing , redness , oedema and/or hypertrophy ) 
 4  severe inflammation ( marked redness and oedema / hypertrophy , spontaneous bleeding or ulceration ) further followup evaluations occurred at 4weeks , 3months and 6months postsurgery . photos of the test sites and clinical measurements of remaining incision line gaps ( if any ) were obtained , and inflammation was assessed . oral hygiene instructions were reviewed throughout the study . at 1 , 3 and 6 months , dental cleanings were performed . throughout the study , any changes in medications or adverse events were noted . at 6months postsurgery , the test sites were reentered for implant placement . prior to implant placement , vertical and horizontal ridge dimensions were recorded using the indexed measuring stents . to retrieve biopsies approximately 810  mm in length , 3  mm trephines ( ace surgical supply , bur trephine 35 tl 2id 2.8od ti 18.5cl biopsies were retrieved from the implant sites , unless an implant was placed in original , nongrafted bone , in which case the biopsy was retrieved from the grafted area immediately adjacent to the implant , as indicated by the indexed stent . biopsies were retained within their respective trephines , wrapped with lintfree gauze ( the open ends of the trephines serving as the apical orientation reference ) and preserved in 10% buffered formalin solution . trephines together with biopsies were fixed in 4% formalin for 57  days , dehydrated in serial steps of ethanol ( 70% , 80% , 90% , 100% ) , remaining for 1  day in each concentration , and degreased for 1  day in xylene . specimens were then infiltrated , embedded and polymerized ( technovit 9100heraeus kulzer , wehrheim , germany ) . after polymerization , samples were hemisected and cut in 500  m sections using a precision cutting machine ( secotom 50 , stuers , ballerup , denmark ) , so that two sections were obtained per biopsy . the sections were mounted onto opacified acrylicslides and ground to a final thickness of approximately 60  m on a rotating grinding plate ( stuers , ballerup , denmark ) . specimens were subsequently stained with azure ii and pararosaniline ( axio imager m1 and axiocam hrc , carl zeiss , gttingen , germany ) . histologic evaluation was performed with a microscope equipped with a digital analyzer ( axio imager m1 and axiocam hrc , carl zeiss , gttingen , germany ) . histomorphometric analyses were performed with imaging software ( analysis five  soft imaging system , mnster , germany ) on composite overview scans . the area of new healing ( versus old / original bony tissues ) was demarcated in each section . within this area , prior to initiation of the study , using an indexed stent , stone cast model of an extraction socket exhibiting a substantial buccal dehiscence and a unc 15 periodontal probe ( hufriedy mfg . co. , chicago , il , usa ) , all investigators were calibrated ( 1  mm ) to the principal investigator ( ets ) . the first step in the analysis was to determine if the randomization resulted in balanced groups . age , gender , race , ethnicity , bmi and all baseline clinical variables were tested to determine if the two groups were balanced . the analyses were based on the variable types , with dichotomous variables tested using fisher exact test and categorical variables using chisquare test and ttests for continuous variables . the primary hypothesis was that dbbmc  +  nbcm ( test ) was not inferior to dfdba  +  recxc ( control ) in the preservation of ridge volume as measured by the difference in the horizontal distance from stents , buccolingually to the interior ( lingual ) bony wall . the noninferiority margin was 1.5  mm , as obtained from the approximate deviation in ridge dimension observed by wood and mealey . twosample ttests were used to evaluate noninferiority , such that : 
 h0 :  
 ck     
 ob     
 o ha :  
 ck     
 ob  >   
 o ck  =  the mean change in ridge volume for dbbmc  +  nbcm from baseline to 6  months 
  
 ob  =  the mean change in ridge volume for dfdba  +  recxc from baseline to 6  months 
  
 o  =  hypothesized mean difference  =  0.0  mm 
   =  noninferiority margin  =  1.5  mm 
   =  0.05 , onesided1  =  0.90 
 
  
 ck  =  the mean change in ridge volume for dbbmc  +  nbcm from baseline to 6  months 
  
 ob  =  the mean change in ridge volume for dfdba  +  recxc from baseline to 6  months 
  
 o  =  hypothesized mean difference  =  0.0  mm 
   =  noninferiority margin  =  1.5  mm if the test for noninferiority was statistically significant , then a twosample ttest for superiority was subsequently conducted , as detailed in the methodology of morikawa & yoshida ( 1995 ) . treatment differences in the vertical ridge preservation changes ( baseline to 6  months ) , measured apicocoronally from the outside of the stent to the apical extent of the lingual and buccal walls  were also evaluated using the noninferiority method listed above . differences in histomorphometric variables were tested with twosample ttest for superiority due to the exploit nature of the variables at 6  months . due to multiple testing for the five histomorphometric variables , the significance level had to be less  than 0.05/6  =  0.008 ( bonferroni ) . for histomorphometric differences to be clinically significant , a margin of at least 15.0% was needed based on the work of wood and mealey . the behaviour of incision line closure over time was an additional goal of the analysis , with evaluations at 1  week , 1  month and at 3  months . inferiority testing could not be done due to a lack of historical data and the potential time dependence of the data . in addition to mean differences tested with ttests , the frequency of closed incisions was tested at each time point with fisher 's exact test . sample size was predicated on assuring that the primary study objective had adequate power to assess the noninferiority hypothesis . under these assumptions , proc power ( sas version 9.2 , sas institute , inc , cary , north carolina , usa ) required a sample size of 18 evaluable subjects in each treatment group . therefore , a sample size of 40 subjects was considered sufficient to meet the primary objective of this study . although the study was powered for the primary outcome variable , the secondary outcome variables each retained 90% power to demonstrate noninferiority , assuming that the treatments were , in truth , equal forty subjects were enrolled and treated as per protocol , though one randomization code was misinterpreted so that there were 21 dfdba  +  recxc and 19 dbbmc  +  nbcm cases . the misinterpreted randomization code patient results were within the range of results reported . in all cases , grafting and soft tissue management were accomplished as directed by protocol ( fig .  2 ) . left column dfdba  +  recxc and right column dbbmc  +  nbcm . top to bottom ; original extraction socket illustrating extent of vertical and mesialdistal dehiscences , graft placement , membrane coverage , closure , soft tissue appearance at 6  months , ridge preservation at 6  months . at 6  months , of the 40 sites treated , all yielded areas that could be biopsied . thirtyseven sites were deemed , according to the treating investigators , to have sufficient ridge preservation for implant placement . the three sites with insufficient ridge volume occurred at three different investigation centres and were all from the dfdba  +  recxc treatment group . baseline extraction socket defect measures were similar with no significant difference between dfdba  + recxc and dbbmc  +  nbcm treatment sites . soft tissue was approximated only , which left 36 of 40 sites open for secondary healing . note : investigator centre effects were also examined and did not influence results or change significance levels . a list of teeth treated according to investigation centre is provided in table  1 . as required by protocol , all extraction sites included buccal dehiscences greater than  the vertical and mesialdistal extraction socket dimensions . in fact , average dehiscences were approximately  of the extraction socket vertical and mesialdistal dimensions . investigator centres and tooth sites evaluated baseline defect measures were comparable ( not significantly different ) between the two therapies . vertical ridge preservation changes from baseline to 6  months were also not significantly different ; however , horizontal changes were significantly different . boxplots reveal the trend in bony ridge preservation differences between therapies , with dbbmc  +  nbcm providing median , first and third quartile values 13  mm greater in all dimensions . however , given the wide range of results obtained ( see whiskers ) , only buccallingual ridge preservation results were statistically different age , gender , race , ethnicity and bmi were also comparable , though the dbbmc  +  nbcm treatment group had more lifetime tobacco use ( 10 of 19 subjects versus 4 of 21 dfdba  +  recxc subjects ) . there was no difference in dental histories , though one of the dfdba  +  recxc subjects had a history of diabetes , which was deemed  controlled . there was no significant difference between treatment group subject oral hygiene compliance at any time point , though two dfdba  +  recxc subjects were recorded as not compliant at 1  week and one at 1  month , with all dbbmc  +  nbcm subjects compliant throughout the study . horizontal changes from baseline to 6  months , as measured using stents , were significantly different between the two modalities , with dbbmc +  nbcm sites , on average , providing more bony width ( 1.76 mm ) . from baseline to 6  months , vertical bone changes , measured at both buccal and lingual walls , were not significantly different between the two treatment modalities , although dbbmc  +  nbcm sites , on average , achieved more vertical ridge preservation ( fig . complete trephine biopsy sections ( original magnification 50x , azure ii and pararosaniline ) for dfdba  +  recxc ( left pair ) and dbbmc  +  nbcm ( right pair ) , showing both original staining and digital labelling for histomorphometry . ( note that the split in the dbbmc  +  nbcm section was artifactual . ) yellow lines in the lateral regions delineate old bone ( ob ) from new bone ( nb ) and define the healing area of the defects , which were further labelled for the following tissue types : ( 1 ) red for nb 
 not in contact with graft , ( 2 ) pink for nb in contact with graft . dark blue for dfdba 
 not in contact with nb , and light blue for dfdba in contact with nb . light green for dbbmc 
 not in contact with nb , and dark green for dbbmc in contact with nb ( composite overview scans , individual microphotographs original magnification x50 ) . there was no significant difference in inflammation between treatment modalities at any postoperative time point . however , inflammation was higher at 1  week compared with later time points , and , on average , was higher for dfdba  +  recxc , with more  2  and  3  inflammation levels recorded ( 13 dfdba  + recxc sties versus 8 dbbmc  + nbcm sites ) . at 1  month , though overall inflammation scores decreased for both therapies , dfdba  +  recxc still involved more mild , localized inflammation than dbbmc  +  nbcm ( 13 versus 7 ) . by 3  months , inflammation was ranked as inflammation at 1  week was nearly significantly correlated ( spearman correlation 0.0573 pvalue ) with horizontal ridge change over 6  months , in an inverse relationship , i.e. more inflammation tended to produce poorer ridge preservation results . at baseline ( surgical closure ) , incision line gaps were not significantly different between the two treatments , but at 1  week , the buccallingual gaps were , on average , 1.82  mm greater for dfdba  + recxc sites . by 1  month , gap differences were statistically and significantly different ( approximately 1.5  mm difference table  3 ) . additionally , at 1  month , the frequency of closed incision gaps was significantly higher for the dbbmc + nbcm group ( 14 out of 19 ) versus the dfdba  +  recxc ( 8 out 21 ) p  =  0.0309 . the frequency of closed incision lines was not different at either 1  week ( most still open in both groups ) or 3  months ( most closed in both groups ) . though starting out with no significant difference ( baseline , following surgical closure ) , incision line gaps were significantly greater by 1  month for dfdba  +  recxc . median values were approximately 1.52  mm greater for dfdba  +  recxc in both mesialdistal and buccallingual dimensions , though there was a wide range ( boxplot whiskers ) of gap measures for both therapies all biopsies ( n  =  40 ) were successfully retrieved , processed , sectioned and digitally labelled for histomorphometry . graft remnants were embedded either in bone marrow / connective tissue or juxtaposed with new bone trabeculae . in the lower ( apical ) portions of the sections , graft remnants were most often directly encompassed with or in contact with new bone . in the coronal portion of the sections , connective tissue / bone marrow tended to be more prevalent . dfdba grafting biomaterial observed prior to implantation appeared to be a combination of both mineralized and demineralized bone , including possible nuclear material . after 6  months , dfdba biopsies tended to show signs of remineralization emanating from the mineralized portions of the grafts , and around this remineralization zone , osteocyte lacunae sometimes appeared to be the nidi for remineralization  islands . occasionally , osteoclasts could be seen resorbing the graft mineral  a phenomenon not observed with dbbmc grafts . dbbmc granules , which were more prevalent than dfdba remnants , tended to form a dense trabecular network with new bone ( fig .  4 ) . circular and elliptical outlines indicated endothelial structures of vascularization in both the apical and coronal portions of the sections , with the coronal portions also containing osteoid and osteoblasts . osteonlike structures were sometimes , but rarely , seen within the dbbmc granules . ( a ) dfdba graft prior to implantation showing  different degrees of mineralization within the  virgin  grafting material : fully  mineralized bone ( mdb ) , partially demineralized bone ( pdb ) , and almost completely  demineralized bone ( ddb ) , including osteocyte lacunae ( ol ) empty or filled with organic material . ( b ) six month biopsy showing remineralization of dfdba : demineralized dfdba ( ddb ) , mineralized dfdba ( mdb ) , remineralized dfdba ( rdb ) , and  islandlike calcified structures ( i ) in the remineralization zone . ( c ) resorption ( r ) of dfdba by osteoclast ( oc )  a phenomenon not observed with dbbmc . ( d ) original , native bone ( ob ) with dbbmc ( bb ) embedded in connective tissue ( ct ) or in newly formed bone ( nb ) . ( e ) a possible vascular channel in dbbmc within the coronal portion of the biopsy . ( f ) woven new bone ( wnb ) with tightly integrated dbbmc ( bb ) granules forms a dense trabecular network ; loose connective tissue is free of inflammation and densely vascularized . mean and standard deviation values for the percentages of each tissue type and graft remnants are presented in table  4 . only tissues ( and graft ) within the extraction socket defect were taken into account ; older original bone was excluded . accordingly , the sum of new bone , connective tissue / bone marrow and graft equalled the entire area measured , or 100% . relative area of biopsy section tissue components ( % ) , n  =  40 the percentage of new bone formed was not significantly different between treatment modalities , though there were significantly more graft remnants within the dbbmc sites , and new bone in contact with graft remnants was , accordingly , greater for dfdba . overall , there were no statistical differences between the two therapies either in the percentage of graft remnants in contact with new bone or in the overall percentage of trabecular network formed by the combination of new bone and grafts . there was no statistical difference in baseline bony wall thicknesses between test and control therapies , but both the buccal and lingual baseline wall thicknesses were related to the amount of ridge preserved vertically and horizontally at 6  months ; inversely , smaller bony wall thicknesses were related to better ridge preservation results . both were significant ( buccal p    0.02 ; lingual p    0.01 ) . there was no significant relationship found between the size of the bony wall dehiscences at baseline and the ridge preservation achieved at 6  months . however , the baseline extraction socket measurement  crest to apical extent of socket  ( which was also not statistically different between test and control therapies ) was related to the 6  month ridge area changes , pvalue  0.03 . the relationship was positive , i.e. deeper baseline defects were associated with better ridge preservation at 6  months . horizontal changes from baseline to 6  months , as measured using stents , were significantly different between the two modalities , with dbbmc +  nbcm sites , on average , providing more bony width ( 1.76 mm ) . from baseline to 6  months , vertical bone changes , measured at both buccal and lingual walls , were not significantly different between the two treatment modalities , although dbbmc  +  nbcm sites , on average , achieved more vertical ridge preservation ( fig .  3 ) . complete trephine biopsy sections ( original magnification 50x , azure ii and pararosaniline ) for dfdba  +  recxc ( left pair ) and dbbmc  +  nbcm ( right pair ) , showing both original staining and digital labelling for histomorphometry . ( note that the split in the dbbmc  +  nbcm section was artifactual . ) yellow lines in the lateral regions delineate old bone ( ob ) from new bone ( nb ) and define the healing area of the defects , which were further labelled for the following tissue types : ( 1 ) red for nb 
 not in contact with graft , ( 2 ) pink for nb in contact with graft . dark blue for dfdba 
 not in contact with nb , and light blue for dfdba in contact with nb . light green for dbbmc 
 not in contact with nb , and dark green for dbbmc in contact with nb ( composite overview scans , individual microphotographs original magnification x50 ) . there was no significant difference in inflammation between treatment modalities at any postoperative time point . however , inflammation was higher at 1  week compared with later time points , and , on average , was higher for dfdba  +  recxc , with more 2  and  3  inflammation levels recorded ( 13 dfdba  + recxc sties versus 8 dbbmc  + nbcm sites ) . at 1  month , though overall inflammation scores decreased for both therapies , dfdba  +  recxc still involved more mild , localized inflammation than dbbmc  +  nbcm ( 13 versus 7 ) . by 3  months inflammation at 1  week was nearly significantly correlated ( spearman correlation 0.0573 pvalue ) with horizontal ridge change over 6  months , in an inverse relationship , i.e. more inflammation tended to produce poorer ridge preservation results . at baseline ( surgical closure ) , incision line gaps were not significantly different between the two treatments , but at 1  week , the buccallingual gaps were , on average , 1.82  mm greater for dfdba  + recxc sites . by 1  month , gap differences were statistically and significantly different ( approximately 1.5  mm difference additionally , at 1  month , the frequency of closed incision gaps was significantly higher for the dbbmc  + nbcm group ( 14 out of 19 ) versus the dfdba  +  recxc ( 8 out 21 ) p  =  0.0309 . the frequency of closed incision lines was not different at either 1  week ( most still open in both groups ) or 3  months ( most closed in both groups ) . though starting out with no significant difference ( baseline , following surgical closure ) , incision line gaps were significantly greater by 1  month for dfdba  +  recxc . median values were approximately 1.52  mm greater for dfdba  +  recxc in both mesialdistal and buccallingual dimensions , though there was a wide range ( boxplot whiskers ) of gap measures for both therapies all biopsies ( n  =  40 ) were successfully retrieved , processed , sectioned and digitally labelled for histomorphometry . graft remnants were embedded either in bone marrow / connective tissue or juxtaposed with new bone trabeculae . in the lower ( apical ) portions of the sections , graft remnants were most often directly encompassed with or in contact with new bone . in the coronal portion of the sections , connective tissue / bone marrow tended to be more prevalent . dfdba grafting biomaterial observed prior to implantation appeared to be a combination of both mineralized and demineralized bone , including possible nuclear material . after 6  months , dfdba biopsies tended to show signs of remineralization emanating from the mineralized portions of the grafts , and around this remineralization zone , osteocyte lacunae sometimes appeared to be the nidi for remineralization  islands . occasionally , osteoclasts could be seen resorbing the graft mineral  a phenomenon not observed with dbbmc grafts . dbbmc granules , which were more prevalent than dfdba remnants , tended to form a dense trabecular network with new bone ( fig . circular and elliptical outlines indicated endothelial structures of vascularization in both the apical and coronal portions of the sections , with the coronal portions also containing osteoid and osteoblasts . osteonlike structures were sometimes , but rarely , seen within the dbbmc granules . ( a ) dfdba graft prior to implantation showing  different degrees of mineralization within the  virgin  grafting material : fully  mineralized bone ( mdb ) , partially demineralized bone ( pdb ) , and almost completely  demineralized bone ( ddb ) , including osteocyte lacunae ( ol ) empty or filled with organic material . ( b ) six month biopsy showing remineralization of dfdba : demineralized dfdba ( ddb ) , mineralized dfdba ( mdb ) , remineralized dfdba ( rdb ) , and  islandlike calcified structures ( i ) in the remineralization zone . ( c ) resorption ( r ) of dfdba by osteoclast ( oc )  a phenomenon not observed with dbbmc . ( d ) original , native bone ( ob ) with dbbmc ( bb ) embedded in connective tissue ( ct ) or in newly formed bone ( nb ) . ( e ) a possible vascular channel in dbbmc within the coronal portion of the biopsy . ( f ) woven new bone ( wnb ) with tightly integrated dbbmc ( bb ) granules forms a dense trabecular network ; loose connective tissue is free of inflammation and densely vascularized . mean and standard deviation values for the percentages of each tissue type and graft remnants are presented in table  4 . only tissues ( and graft ) within the extraction socket defect were taken into account ; older original bone was excluded . accordingly , the sum of new bone , connective tissue / bone marrow and graft equalled the entire area measured , or 100% . relative area of biopsy section tissue components ( % ) , n  =  40 the percentage of new bone formed was not significantly different between treatment modalities , though there were significantly more graft remnants within the dbbmc sites , and new bone in contact with graft remnants was , accordingly , greater for dfdba . overall , there were no statistical differences between the two therapies either in the percentage of graft remnants in contact with new bone or in the overall percentage of trabecular network formed by the combination of new bone and grafts . there was no statistical difference in baseline bony wall thicknesses between test and control therapies , but both the buccal and lingual baseline wall thicknesses were related to the amount of ridge preserved vertically and horizontally at 6  months ; inversely , smaller bony wall thicknesses were related to better ridge preservation results . both were significant ( buccal p    0.02 ; lingual p    0.01 ) . there was no significant relationship found between the size of the bony wall dehiscences at baseline and the ridge preservation achieved at 6  months . however , the baseline extraction socket measurement  crest to apical extent of socket  ( which was also not statistically different between test and control therapies ) was related to the 6  month ridge area changes , pvalue  0.03 . the relationship was positive , i.e. deeper baseline defects were associated with better ridge preservation at 6  months . this investigation compared two ridge preservation methodologies in substantial buccal wall dehiscence defects . since bone remodelling and soft tissue collapse and their interference with bony healing are common in these defects , they provide a critical methodological test . one technique ( dfdba  +  rexc ) was derived from the history of demineralized allograft use and the belief that such allografts might be osteoinductive and produce bone formation . dfdba was combined with a reconstituted and crosslinked collagen membrane thought to better preserve ( through stiffness ) the volume intended for regeneration , and for a longer time than noncrosslinked collagen membranes . the other technique ( dbbmc  +  nbcm ) was derived from a history of mineralized xenogeneic graft use and the belief that such grafts might be osteoconductive , giving rise to bone integration , and yet resorb slowly to preserve the volume intended for regeneration . dbbmc was combined with an extracted and noncrosslinked collagen membrane thought to encourage transmembrane vascularity and rapid tissue integration , with the membrane degrading over a time period thought sufficient for bone regeneration . for the primary outcome of ridge preservation , horizontal ridge preservation was significantly greater for dbbmc  +  nbcm . there was only a trend , and not statistically significant , for more vertical bone preservation with dbbmc  +  nbcm . as a clinical consequence , implants could be placed in all but three treatment sites , which were dfdba+  recxc sites . ( 2015 ) , he postulated that , following tooth extraction ,   ( i ) up to 50% reduction in the original ridge width will occur ; ( ii ) the amount of bone resorption will be greater at the buccal aspect than at its lingual / palatal counterpart ; and ( iii ) a larger amount of alveolar bone reduction will take place in the molar regions .  in this review of clinical studies with various biomaterials , it was concluded that ridge contraction following tooth extraction can be minimized with socket grafts and/or the use of mechanical barriers . while grafts alone might be sufficient for spacemaintaining sockets , others have found grafts plus membranes to be advantageous , particularly for limited spacemaintaining defects like the dehiscence defects studied herein ( kim et  al . the secondary outcomes , based on histomorphometry , incision line gap closure and degree of inflammation , were included in the hope they might help explain the primary outcome of ridge preservation . indeed , the percentage of new bone seen in the biopsy sections was not statistically different between the two therapies studied . this , in turn , may support the premise that minimal dbbmc resorption provided spacemaintenance for improved ridge volume ( buser et  al . 2014 ) . moreover , there was no difference in the percentage of trabecular structure ( new bone plus graft ) observed between the two therapies . dbbmc biopsies showed little to no signs of osteoclastic resorption and graft remodelling ; rather , dbbmc was intimately incorporated into new bone trabeculae . however , over time , particularly in the coronal regions where bone formation appeared less advanced than in apical regions , studies of dbbmc in the sinus have indicated woven bone turns into lamellar bone , along with further bone formation , integration and maturation ( sartori et  al . dfdba biopsies depicted robust bone formation with islands of new bone that might be interpreted as evidence of bone induction ; but in contrast to dbbmc , dfdba appeared to be in a more active state of turnover and replacement . this boneremodelling phenomenon has also been observed in the maxillary sinus ( soardi et  al . 2013 )  a phenomenon that might help explain the difference in ridge volume preservation observed between the two therapies in this study . examining soft tissue healing , the spearman correlation , pvalue of 0.0573 , just on the edge of significance , was a strong pvalue , considering inflammation was a categorical and subjective variable . more inflammation was observed , by count , at dfdba  +  recxc sites . of the 18 sites with incision line gaps still open at 1  month , 13 were dfdba  +  recxc . early vascularization and soft tissue integration of nbcm membranes , as compared with crosslinked collagen membranes , may have improved soft tissue healing and underlying bony tissue regeneration results , with nbcm membranes degrading over a time period reported as sufficient for guided regeneration ( bornstein et  al . 2007 , schwarz et  al . 2008 ) . following the hypothesis that gbr was simply a consequence of tissue separation and that the volume created by the membranes determined the volume of bone that could be regenerated , stiffer , titanium reinforced and shapeable expanded polytetrafluoroethylene ( eptfe ) membranes were developed ( schenk et  al . these membranes were designed for defects in which there was an absence of residual bony walls , i.e. the defects themselves were not able to prevent membrane collapse into the area intended for gbr . however , eptfe membrane shortcomings included soft tissue dehiscences , along with potential inflammation and infection , recognized as clinical complications that diminished regenerative therapy outcomes ( machtei et  al . , biodegradable collagen membranes were not only designed to integrate with the healing tissues but also to degrade over time , particularly when exposed to the oral environment ( zitzmann et  al . 1997 ) . still , the quest for spacemaintaining membranes and the uncertainty over what might be a suitable time period for degradable membranes to remain intact led to investigations of stiffer and more slowly degrading collagen barriers ( bunyaratavej & wang 2001 ) . despite this history , and even though the historical suggested removal time for eptfe in gbr procedures was after several months of healing , today some researchers speculate that the regenerative disposition of tissues may occur over the course of a few weeks ( susin et  al . membrane integration and duration of resorption , which could be associated with the degree of soft tissue inflammation and incision line gapping reported in the study herein , might help explain the differences observed between the two therapies compared . regardless , given the four biomaterials tested in this study ( two grafts and two membranes ) , it was not possible to isolate cause and effect for any single biomaterial . only the combined effects of the grafts and their respective membranes could be evaluated . other potential design limitations included evaluation of implant integration and survival , the number of investigators and the variety of defect morphologies tested . success of implant integration and longterm survival were not reported herein but will be provided in a subsequent report . despite the potential variability inherent with a large group of investigators , the number of investigators may have provided a more poignant finding , with results that might better represent the clinical community at large . baseline extraction bony wall thickness and extraction socket depth ( crest to apex ) were related to ridge preservation outcomes , and case photos appeared to indicate both of these phenomena involved the regenerative potential of the baseline defects , i.e. remaining bony walls ( measured as  thin  because of their coronal extension and  deep  in extent ) were more spacemaintaining . in future studies , extraction defect morphologies should be further apportioned for more definitive analyses . in addition , studies should be performed in the anterior region , where aesthetics , labial bone loss and soft tissue management are more critical . finally , we recommend , as reported herein , that future ridge preservation studies include substantial , buccal wall dehiscence defects as critical tests of biomaterials , surgical techniques and , ultimately , patient outcomes .