periodontal diseases , the most prevalent diseases throughout the world , are predominantly caused by gram - negative , anerobic bacteria present on the tooth root surfaces as a biofilm . inflamed periodontal tissue produces significant amounts of pro - inflammatory cytokines , mainly il-1 , il-6 , pge2 and tumor necrosis factor alpha ( tnf- ) , reactive oxygen species enzymes , proteins , host cells , ions , hormones , and markers of oxidative stress and antioxidant . reactive oxygen species ( ros ) play crucial roles in normal physiological processes including response to growth factors , the immune response , and apoptotic elimination of damaged cells but are also highly toxic and destructive when generated during the respiratory burst as it represents an important pathogenic mechanism for tissue damage and diseases associated with phagocytic infiltration . the discovery of the role of free radicals in cancer , diabetes , cardiovascular diseases , autoimmune diseases , neurodegenerative disorders , aging , and other chronic diseases has led to a medical revolution comprising of various antioxidants that is promising a new paradigm of healthcare . these are the agents , which scavenge free radicals otherwise reactive oxygen species and prevent the damage caused by them . lycopene , a pigment that gives tomatoes red color , is one of the most potent antioxidants among dietary carotenoids with a singlet - oxygen - quenching ability three times as high as that of  - carotene and ten times higher than that of -tocopherol . dietary intake of tomatoes and tomato products containing lycopene has been shown to be associated with a decreased risk of chronic diseases , such as cancer and cardiovascular disease - tocopherol . dietary intake of tomatoes and tomato products containing lycopene has been shown to be associated with a decreased risk of chronic diseases , such as cancer and cardiovascular disease . various biomarkers apart from clinical appearance have been used to evaluate periodontal destruction which includes enzymes , proteins , host cells , markers of cellular and humoral activity , ions , hormones , and markers of oxidative stress and antioxidants . uric acid is one of the major antioxidants that is present in saliva and can be used to determine the effect of antioxidant therapy in the treatment of periodontal diseases . the aim of the present study is to evaluate efficacy of antioxidants in the treatment of chronic gingival and periodontal disease and to compare the efficacy of antioxidant as a monotherapy and as an adjunct in the treatment of gingivitis and chronic periodontitis . 70 subjects were selected from those attending the department of periodontics , pacific dental college and hospital , udaipur , rajasthan . subjects were selected on the basis of community periodontal index of treatment needs ( cpitn ) . subjects with systemic diseases like diabetes , cardiovascular diseases , and renal diseases , subjects with a habit of smoking , pan chewing , subjects on medications like antibiotics or antioxidants like vitamin c , vitamin e or -carotenes within 3 months and pregnant and lactating mothers were excluded from this study . the proposed study was reviewed by the ethical committee of the institution and clearance was obtained . an informed consent was obtained from each subject before conducting the trial , the form of which is enclosed . the selected subjects were divided into three groups on the basis of cpitn index [ table 1 ] . subgroup iib : 10 subjects were treated by non - surgical periodontal therapy and were given antioxidants also . subgroup iiib : 10 subjects were treated with non - surgical periodontal therapy and were given antioxidants also . antioxidant was given in a dosage of 6 mg per day in three divided doses for 2 weeks . each softgel was composed of lycopene 2000 mcg , zinc 7.5 mg , and selenium 35 mcg . 1 ml of unstimulated saliva samples were collected by allowing saliva to passively flow into a sterile test tube after rinsing the mouth with water to wash out the exfoliated cells for all the subjects . supragingival and subgingival scaling were done after sample collection in groups ii and iii subgroup a and b. the samples of saliva from group ii and iii patients were collected again after 15 , 30 , and 45 days of nonsurgical periodontal treatment . the uric acid level was evaluated by using a reflotron reflectance photometer at the wavelength of 642 nm . 1 ml of unstimulated saliva samples were collected by allowing saliva to passively flow into a sterile test tube after rinsing the mouth with water to wash out the exfoliated cells for all the subjects . supragingival and subgingival scaling were done after sample collection in groups ii and iii subgroup a and b. the samples of saliva from group ii and iii patients were collected again after 15 , 30 , and 45 days of nonsurgical periodontal treatment . the uric acid level was evaluated by using a reflotron reflectance photometer at the wavelength of 642 nm . the mean score for patients of group 1 ( clinically healthy ) was 0.780.26 , 1.870.27 for group 2 ( gingivitis group ) , and 6.110.58 for group 3 ( periodontitis group ) . on comparison between 1 and 2 , 2 and 3 , and 1 and 3 using post hoc test , highly significant difference ( p=0.000 ) was observed on 0 day . one - way anova test was applied to compare the periodontal status between and within the groups on 0 , 15 , 30 , and 45 day . comparison between 0 , 15 , 30 , and 45 days showed a highly significant difference [ table 1 ] . for patients of group 1 , the mean score on 0 day was 5.190.8 and 5.560.88 on the 45 day . on comparison between 0 and 45 day using the t test , no significant difference ( p=0.15 ) was observed [ table 2 ] . comparison of the russell periodontal index between all the groups and subgroups on baseline , 15 , 30 , and 45 day for patients of subgroup a , the mean score was 3.160.32 , subgroup b the mean score was 2.250.35 , subgroup c the mean score was 2.20.26 . on comparison between a and b using the post hoc test , no significant difference ( p=0.7 ) was observed while a highly significant difference ( p=0.00 ) was observed between subgroups b & c and a & c using the post hoc test , on 0 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 2 on 0 , 15 , 30 , and 45 day [ figure 1 ] . on comparison of uric acid on 0 , 15 , and 30 day , significant difference ( p=0.00 , 0.001 and 0.009 , respectively ) was observed . while the uric acid level on 45 day showed non - significant difference ( p=0.53 ) . comparison of uric acid levels between subgroup a , b and c of group 2 on 0 , 15 , 30 and 45 day for patients of subgroup a , the mean score was 2.430.42 , subgroup b the mean score was 2.290.4 , and subgroup c the mean score was 2.290.39 . on comparison between a and b , b and c , and a and c using post hoc test , no significant difference ( p=0.65 , 0.99 , and 0.45 respectively ) was observed on 0 day . on comparison between a and b using the post hoc test , no significant difference ( p=0.26 ) was observed . on comparison between b and c and a and c using the post hoc test , significant difference was observed on 15 , 30 , and 45 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 3 on 0 , 15 , 30 , and 45 day [ figure 2 ] . on comparison of uric acid on 15 , 30 , and 45 day , significant difference ( p=0.042 , 0.024 , and 0.00 , respectively ) was observed . comparison of uric acid levels between subgroup a , b and c of group 3 on 0 , 15 , 30 and 45 day the mean score for patients of group 1 ( clinically healthy ) was 0.780.26 , 1.870.27 for group 2 ( gingivitis group ) , and 6.110.58 for group 3 ( periodontitis group ) . on comparison between 1 and 2 , 2 and 3 , and 1 and 3 using post hoc test , highly significant difference ( p=0.000 ) was observed on 0 day . one - way anova test was applied to compare the periodontal status between and within the groups on 0 , 15 , 30 , and 45 day . comparison between 0 , 15 , 30 , and 45 days showed a highly significant difference [ table 1 ] . for patients of group 1 , the mean score on 0 day was 5.190.8 and 5.560.88 on the 45 day . on comparison between 0 and 45 day using the t test , no significant difference ( p=0.15 ) was observed [ table 2 ] . comparison of the russell periodontal index between all the groups and subgroups on baseline , 15 , 30 , and 45 day for patients of subgroup a , the mean score was 3.160.32 , subgroup b the mean score was 2.250.35 , subgroup c the mean score was 2.20.26 . on comparison between a and b using the post hoc test , no significant difference ( p=0.7 ) was observed while a highly significant difference ( p=0.00 ) was observed between subgroups b & c and a & c using the post hoc test , on 0 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 2 on 0 , 15 , 30 , and 45 day [ figure 1 ] . on comparison of uric acid on 0 , 15 , and 30 day , significant difference ( p=0.00 , 0.001 and 0.009 , respectively ) was observed . while the uric acid level on 45 day showed non - significant difference ( p=0.53 ) . comparison of uric acid levels between subgroup a , b and c of group 2 on 0 , 15 , 30 and 45 day for patients of subgroup a , the mean score was 2.430.42 , subgroup b the mean score was 2.290.4 , and subgroup c the mean score was 2.290.39 . on comparison between a and b , b and c , and a and c using post hoc test , no significant difference ( p=0.65 , 0.99 , and 0.45 respectively ) was observed on 0 day . on comparison between a and b using the post hoc test , no significant difference ( p=0.26 ) was observed . on comparison between b and c and a and c using the post hoc test , significant difference was observed on 15 , 30 , and 45 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 3 on 0 , 15 , 30 , and 45 day [ figure 2 ] . on comparison of uric acid on 15 , 30 , and 45 day , significant difference ( p=0.042 , 0.024 , and 0.00 , respectively ) was observed . comparison of uric acid levels between subgroup a , b and c of group 3 on 0 , 15 , 30 and 45 day for patients of group 1 , the mean score on 0 day was 5.190.8 and 5.560.88 on the 45 day . on comparison between 0 and 45 day using the t test , no significant difference ( p=0.15 ) was observed [ table 2 ] . comparison of the russell periodontal index between all the groups and subgroups on baseline , 15 , 30 , and 45 day for patients of subgroup a , the mean score was 3.160.32 , subgroup b the mean score was 2.250.35 , subgroup c the mean score was 2.20.26 . on comparison between a and b using the post hoc test , no significant difference ( p=0.7 ) was observed while a highly significant difference ( p=0.00 ) was observed between subgroups b & c and a & c using the post hoc test , on 0 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 2 on 0 , 15 , 30 , and 45 day [ figure 1 ] . on comparison of uric acid on 0 , 15 , and 30 day , significant difference ( p=0.00 , 0.001 and 0.009 , respectively ) was observed . while the uric acid level on 45 day showed non - significant difference ( p=0.53 ) . comparison of uric acid levels between subgroup a , b and c of group 2 on 0 , 15 , 30 and 45 day for patients of subgroup a , the mean score was 2.430.42 , subgroup b the mean score was 2.290.4 , and subgroup c the mean score was 2.290.39 . on comparison between a and b , b and c , and a and c using post hoc test , no significant difference ( p=0.65 , 0.99 , and 0.45 respectively ) was observed on 0 day . on comparison between a and b using the post hoc test , no significant difference ( p=0.26 ) was observed . on comparison between b and c and a and c using the post hoc test , significant difference was observed on 15 , 30 , and 45 day . one - way anova test was applied to compare uric acid levels between and within the subgroups of group 3 on 0 , 15 , 30 , and 45 day [ figure 2 ] . on comparison of uric acid on 15 , 30 , and 45 day , significant difference ( p=0.042 , 0.024 , and 0.00 , respectively ) was observed . comparison of uric acid levels between subgroup a , b and c of group 3 on 0 , 15 , 30 and 45 day the inflammatory and immune responses to the bacteria and also viruses that colonize the periodontal and associated tissues involve the systemic circulation and ultimately the peripheral systems of the body . this creates a complex bi - directional series of host - microbial interactions involving cellular and humoral factors and networks of cytokines , chemokines , and growth factors . according to haffajee , et al . , the primary etiological agent is specific which consists predominantly gram - negative anerobic or facultative bacteria within the subgingival biofilm , but the majority of periodontal tissue destruction is caused by an inappropriate host response to those microorganisms and their products . detection of markers of tissue destruction can thus be incorporated in diagnosis of periodontal diseases . loss of balance between reactive oxygen species and antioxidant defense has also been implicated as an etiologic factor for periodontal diseases . it may manifest as an increase in oxidative stress , reduction of total antioxidant capacity , or decrease in individual antioxidant level . chapple and matthews implicated oxidative stress in the pathogenesis of periodontitis . in this study , significant difference was observed in the periodontal index between healthy , gingivitis , and periodontitis group on 0 day and 15 day . similar results were also observed in a study by cugini , et al . according to moore , et al . , uric acid is a major antioxidant in saliva and contributes approximately 70% of the total salivary antioxidant capacity . it has been seen that levels of antioxidants vary according to the severity of disease , and similarly uric acid levels also vary according to the severity of periodontal disease . significant difference was observed in salivary uric acid levels on 0 day between healthy , gingivitis and periodontitis groups . a decrease in the salivary uric acid levels were observed in the gingivitis and periodontitis groups . diab - ladki , et al . in a similar study also found that there was a slight decrease in the concentration of three main antioxidants ( uric acid , ascorbic acid , and albumin ) in saliva with the increase in severity of periodontal disease . results of this study showed that there was a significant increase in uric acid levels in all the subgroups a , b , and c [ tables 1 and 3 ] on 15 , 30 , and 45 day of both gingivitis and periodontitis groups . comparison of uric acid levels of group 1 on 0 and 45 day although no significant difference could be observed in uric acid levels between scaling and root planing ( subgroup a ) and scaling and root planing with antioxidant ( subgroup b ) , an increase in the mean uric acid levels was observed as the time elapsed . chandra , et al . in their study observed that though there was no significant difference in uric acid levels in between groups treated with a combination of oral prophylaxis and antioxidant and group treated with scaling and root planning alone , there was a better response to antioxidant therapy in combination to oral prophylaxis in the gingivitis group . in this study , no significant difference was seen in salivary uric acid levels from 30 to 45 day . this shows that within 45 days , a plateau plasma level of antioxidants was achieved which causes decrease in values of the periodontal index . there was a reduction in periodontal inflammation with an increase in the salivary uric acid levels seen in subgroups treated by antioxidants in both gingivitis and periodontitis groups . hence , within the limitations of this study , it can be concluded that antioxidants can be used as an effective adjunct to scaling and root planing for the treatment of gingivitis and periodontitis . to further evaluate long - term effects of antioxidant therapy on periodontal inflammation , more expanded