our aim in dpp was to prevent diabetes by some lifestyle measure or even medication interventions among at risk individuals ( ifg / igt patients ) . in the dpp , the first group was the lifestyle intervention that received intensive training in diet and physical activity . our aim was , losing 7 percent of their body weight and maintaining that loss . males and females 30 to 55 year of age who were first relative history of type 2 diabetes were invited to study via community announcement . this population - based cross - sectional study was performed among 1450 people with first relative history of diabetes ( f.h.d ) . after excluding peoples using medications that would influence serum lipids , current analysis was done among 1323 individuals with f.h.d ( 332 males and 991 females ) . the criterion for participants was having1 first - degree relative with a diagnosis of diabetes after 30 years old . this study was approved by the research council of endocrine and metabolism research center ( emrc ) of isfahan university of medical science and each participant filled in consent . anthropometric assessment : weight was measured by seca scale while subjects were lightly clothed and without shoes and recorded to the nearest .1 kg . height was measured by seca stadiometer while subjects were in a standing position without shoes and their shoulders were in a normal position . body mass index was calculated as weight ( in kg ) divided by height square ( in m2 ) . wc was measured at minimal waist after a normal exhale and hip circumference was measured at the widest point over the buttocks with an un - scratched tape measure and without any pressure to body surface ; measurements were recorded to the nearest 1 cm . data on physical activity were gathered by using participants responses to one query : how much time do you exercise in a week ? we categorized the replies of this query as never , < 3 h / wk , and  3 h / wk . definition of terms : participants were categorized in 4 phenotype groups on the basis of the mentioned cutoff points : nwnt ( normal waist normal triglyceride ) : normal wc < 102 for man and for women < 88 and normal serum triglyceride concentrations ( < 150 mg / dl ) . ewnt ( enlarged waist normal triglyceride ) : enlarged wc for men  102 and for women  88 and normal serum triglyceride concentration ( < 150 mg / dl ) . nwet ( normal waist elevated triglyceride ) : normal wc for men < 102 and for women < 88 and elevated triglyceride  150 mg / dl . ewet ( enlarged waist elevated triglyceride ) : enlarged wc for men  102 and for women  88 and hyper serum triglyceride concentration (  150mg / dl ) . biochemical assessment : blood samples were taken after>10h overnight fasting while the participants were in a sitting and relax position and were centrifuged within 5 min . the samples was analyzed by using autoanalyzer bt 3000 ( rome , italy ) by enzymatic glucose method using commercial kits ( chem enzyme , tehran , iran ) . serum cholesterol and triglyceride levels were measured by enzymatic reagents ( chem enzyme , tehran , iran ) adapted to the selecta auto analyzer . high - density lipoprotein cholesterol levels were measured using kits ( pars az - moon , tehran , iran ) . inter - assay coefficients of variations ( cvs ) were 1.25 for tg , 1.2 for cholesterol and 1.2% for glucose . glycated hemoglobin levels ( hba1c ) were assessed with ds5 and ds5 analyzer uses low pressure cation exchange chromatography in conjunction with gradient elution to separate human hemoglobin subtypes and variants from haemolysed whole blood.1920 statistical package for social sciences ( spss ) was used for statistical analyses . general characteristics of individuals across different phenotypes of ewet were compared by oneway analysis of variance ( anova ) with tukey post - hoc correction . multivariate - adjusted means for metabolic variables across various phenotypes of ewet was obtained by the use of analysis of covariance ( ancova ) in four models . in first model , we adjusted age ( y ) and physical activity ( never , < 3 hour / week , and  3 hour / week ) . in the second one chi - square test was used for comparing prevalence of different phenotypes of ewet across different categories of ogtt test . general characteristics of individuals across different phenotypes of ewet were compared by oneway analysis of variance ( anova ) with tukey post - hoc correction . multivariate - adjusted means for metabolic variables across various phenotypes of ewet was obtained by the use of analysis of covariance ( ancova ) in four models . in first model , we adjusted age ( y ) and physical activity ( never , < 3 hour / week , and  3 hour / week ) . in the second one chi - square test was used for comparing prevalence of different phenotypes of ewet across different categories of ogtt test . general characteristics of individuals across different phenotypes of ewet were compared by oneway analysis of variance ( anova ) with tukey post - hoc correction . multivariate - adjusted means for metabolic variables across various phenotypes of ewet was obtained by the use of analysis of covariance ( ancova ) in four models . in first model , we adjusted age ( y ) and physical activity ( never , < 3 hour / week , and  3 hour / week ) . in the second one chi - square test was used for comparing prevalence of different phenotypes of ewet across different categories of ogtt test . mean age of men and women was 43.5  5.5 and 43.0  5.6 y , respectively . mean bmi , wc , whr and tg among men were 27.6  3.3 kg / m2 , 94.4  8.6 cm , 0.9  0.06 and 192.7  110 respectively and among women 29.6  4.5 kg / m2 , 87.9  9.1 cm , 0.8  0.05 and 158  83 respectively . of the 1450 adult 332 ( 9.6% ) of men and 234 ( 23.6% ) of women had ewet phenotype . general characteristics of male subjects separately in different phenotypes of wc and serum triglyceride levels have been presented in table 1 . those with ewet phenotype had higher bmi , wc and whr as compared to other phenotypes ( p < 0.01 for all ) . general characteristics of male participants by different phenotype of serum triacylglycerol concentration and waist circumference women 's general characteristics have been shown in table 2 . those with the ewet phenotype were older and higher bmi , wc and whr as compared to other phenotypes . characteristics female with phenotypes of serum triacylglyceral concentration and waist circumference men with the ewet phenotype had higher levels of serum triglyceride , cholesterol , and lower levels of hdl - cholesterol as compared to other groups ( table 3 ) . such adjustments revealed significant differences in 2h - pg and hba1c between the ewet phenotype and other groups . even after further adjustment for bmi , all the mentioned differences were significant except for serum hdl - cholesterol that was marginally non significant ( p = 0.057 ) . crude means of all metabolic variables were of higher levels among women with the ewet phenotype as compared to other phenotypes ( table 4 ) . controlling for age and physical activity and additional control for bmi had little impacts on these differences and they were still significant . adjusted means for metabolic risk factors across four phenotypes of serum trigcylglycerol concentration and waist circumference in men multivariate  adjusted means for metabolic risk factors across four phenotypes of serum trigcylglycerol concentration and waist circumference in women the prevalence of impaired glucose tolerance ( igt ) and diabetes across different phenotypes of wc and serum triglyceride levels have been provided in table 5 . both men and women with the ewet phenotype had higher prevalence of type 2 diabetes compared to other phenotypes . the prevalence of igt was not significantly different in four phenotypes of wc and serum tg concentration . prevalence of diabetes and impaired glucose toleronce in male and female paticipants across different phenotypes of triacylglycerol conrentration ad waist circumference findings of the current study suggest a positive association between hypertriglyceridemic waist phenotype and diabetes prevalence . we observed that males and females with the ewet phenotype had higher metabolic abnormalities as compared to those without this phenotype . this study is among the first study relating hypertriglyceridemic waist phenotype to igt and diabetesin a sample of individuals with first relative history of diabetes . hypertriglyceridemic waist phenotype has been suggested as a screening tool for metabolic risk factors.21 considering the increasing trend in the prevalence of heart diseases and diabetes all over the world , developing simple and cheap screening tools for early identification of these chronic diseases is of great importance.17 ewet phenotype has the ability to recognize more subjects characterized by the metabolic traid than the presence of the metabolic syn - drome.21 some investigators have used this simple screening tool for the identification of metabolic triad and cardiovascular risk.15 among tehrani people , this phenotype have been reported as a valid predictor of cardiovascular disease , not only among adult,1617 but also among adolescents,22 such that those adolescents with the ewet phenotype had higher prevalence of other metabolic risks as well . this phenotype has also been demonstrated as a simple method for early identification of those at higher risk of diabetes.9 lemieux et al have reported that two simple measures , waist an tg , might be an cheap clinical method identifying males with elevated insulin , apob and sldl concentration , and hence , increased chd and diabetes risk.15 however , this is the first among individuals with first relative history of diabetes relating this phenotype to igt and diabetes , among individual with fhd . therefore , such a simple index can be used by health professionals in health care system to early identify those at higher risk of diabetes . although waist circumference has been used as a screening measure for this purpose , it has been shown that the addition of a biomarker like serum triglyceride to this measure would increase its accuracy , sensitivity and specificity in this regard.22 in the current study , participants with the ewet phenotype had higher prevalence of metabolic risks . although such findings have previously been reported by other investigators , the population studied in previous publications was not individuals with fhd . the prevalence of ewet phenotype was respectively 9.6% and 23.6% among men and women in this study . lemieux et al23 have reported the phenotype among 19% of 907 participants in the quebec health survey . among 3430 french men , the phenotype has been reported to be 12.1%.24 sumner et al25 found 21% of postmenopausal black women , 28.3% of white women and 42.3% of mexican women with this phenotype . in tehran lipid and glucose study , the phenotype has been reported among 19% of adult men17 and 31.9% of women.16 this figure in isfahani women was 24%.26 as it comes from these figures , the prevalence is considerably different in different populations it seems that the pattern of obesity among middle eastern population is something different from those in other places of the world . in other words the so - called middle eastern pattern of obesity is highly prevalent in these countries . this pattern of obesity is characterized by larger fat accumulation in the abdomen , particularly among women , such that women in these countries have higher prevalence of both general and central obesity . different prevalence of this phenotype could also be explained by the use of different cut - off points for wc and serum triglyceride in the studies . the age range of the studied population must also be taken into account . in this study , prevalence of diabetes among those with the ewet phenotype was 30.3% for men and 44.9% for women , respectively . just 18.2% and 15.4% of those with nwnt phenotype were diabetic men and women , respectively . st - piere at al,9 assessing 1190 individuals , introduced ewet phenotype as a predictor of cardiovascular risks among type 2 diabetic and igt patients . they found that nearly 53% of males and 80% of females with the phenotype were diabetics or igt . in a study in us population , 25.4% of those with the phenotype were diabetics and just 8% of diabetics were free of this phenotype.27 however all these studies have been performed among individuals without family history diabetes.all mentioned studies are in line with our findings in the current study suggesting the higher prevalence of diabetes among those with the phenotype . it seems that enlarged waist circumference is associated with increased intra - abdominal fats which in turn could result in higher production of lipid products in the liver and therefore increasing levels of ldl and vldl . on the other hand , high intra - abdominal fat would cause free fatty acid levels to increase in circulation which in turn could result in developing insulin resistance and hyperinsulinemia.22 the gene expression of fat / cd36 as a carrier of long chain fatty acids is regulated by insulin . previous researches have shown that destruction of this protein is closely related to insulin resistance.28 others have shown that the activity of lipoprotein lipase ( lpl ) as a key enzyme for con - trolling serum tg levels is decreased among individuals with insulin resistance.29 increase levels of free fatty acids in beta cells could re - sult in decreased insulin secretions and there - fore increased risks of diabetes.30 some points needed to be considered in the interpretation of our findings . the important limitation of this study is its cross - sectional na - ture which does not allow inferring causal rela - tions . other point that must be kept in mind is the definition of ewet phenotype which might be different from other studies . while the who expert committee31 on physical sta - tus recommends measurement midway be - tween the lower rib and the iliac crest , the nhanes iii guidelines32 prescribe use of a point just above the right ileum and the rec - ommendation of the north american associa - tion for the study of obesity ( naaso ) and the national heart , lung and blood institute ( nhlbi)33 is to use the right iliac crest . the shortage of standard measurement for wc is problem , and makes comparison with other reasearches difficult . it is believed that the use of narrowest waist measurement offers greater ease of acceptance and interpretation by the public and may facilitate self - measurement in addition to clinical use . we measured wc at the point of noticeable waist because no stan - dard location has been reported for waist cir - cumference measuring . we did not measure serum insulin levels and insulin sensitivity of tissues in the current study . therefore further studies in this field are warranted . in conclusion , there was a significant posi - tive association between hypertriglyceridemic waist phenotype and prevalence of diabetes . therefore , using this phenotype for early iden - tification of individuals at high - risk of diabetes has important public health implications for prevention . ma , ms , nm and ma participated in the collection of data , conception and design . ae and mz contributed to conception and design , statistical analysis and data interpretation and manuscript drafting .