gt(rosa)26sor , b6;129-gt(rosa)26sor / j and b6;129s2-scarb1/j and fvb / nj wild - type mice were obtained from the jackson laboratory ( bar harbor , me ) . mice were bred and maintained at the comparative bioscience center of the rockefeller university according to guidelines established by the institutional animal committee . adenoviral constructs encoding human and murine homologues of the four hcv entry factors ( cd81 , scarb1 , cldn1 and ocln ) and mutants thereof were created using the adeasy adenoviral vector system ( agilent technologies , santa clara , ca ) according to the manufacturer s instructions . plasmids encoding chimeric hcv genomes , including jc1flag2(p7fluc2a ) and bicistronic hcv genomes expressing cre , were linearized with xbai and transcribed using megascript t7 ( ambion , austin , texas ) . rna was electroporated into huh-7.5 cells using a ecm 830 electroporator ( btx genetronics ) and infectious virus was collected from supernatants 4872 h after transfection . gt(rosa)26sor , b6;129-gt(rosa)26sor / j and b6;129s2-scarb1/j and fvb / nj wild - type mice were obtained from the jackson laboratory ( bar harbor , me ) . mice were bred and maintained at the comparative bioscience center of the rockefeller university according to guidelines established by the institutional animal committee . adenoviral constructs encoding human and murine homologues of the four hcv entry factors ( cd81 , scarb1 , cldn1 and ocln ) and mutants thereof were created using the adeasy adenoviral vector system ( agilent technologies , santa clara , ca ) according to the manufacturer s instructions . plasmids encoding chimeric hcv genomes , including jc1flag2(p7fluc2a ) and bicistronic hcv genomes expressing cre , were linearized with xbai and transcribed using megascript t7 ( ambion , austin , texas ) . rna was electroporated into huh-7.5 cells using a ecm 830 electroporator ( btx genetronics ) and infectious virus was collected from supernatants 4872 h after transfection .