the transcriptome of medullary stromal cells of patients suffering from primary myelofibrosis was studied by agilent oligo microarray technology . the primary myelofibrosis is a chronic myeloproliferative syndrome . to invest the role of bone marrow stroma in the pathophysiology of this disease , we isolated primary cultured of bone marrow stromal cells from these patients . the osteo - medullary biopsies for the diagnosis of the disease were implanted in dmem medium with 10% fetal calf serum . stromal cells during their proliferation adhere to plastic and they were trypsinized between each passage ( 35 passages ) when cultures came to confluence . a cytometric control was carried out on the cells prior to performing molecular biology experiments . cd105 , cd73 and cd90 marker positivity was verified to validate the mesenchymal cell phenotype . the negativity of the cd45 marker was also carried out to prove the absence of residual hematopoietic cells in culture . each culture of mesenchymal stromal cell is isolated from the bone marrow of an individual . in total , the bone marrow samples were studied individually from 6 healthy donors ( 6 controls : gsm1084994 , gsm1084995 , gsm1084996 , gsm1084997 , gsm1084998 , gsm1084999 ) and from 6 patients with primary myelofibrosis ( 6 pmf : gsm1085000 , gsm1085001 , gsm1085002 , gsm1085003 , gsm1085004 , gsm1085005 ) . concerning the enrollment of control samples : subjects are negative for alcohol abuse and hcv , hbv and hiv virus infections . the choice of control subjects was conditioned by access to bone marrow from subjects having a hip prosthesis surgery : indeed the subjects have an average age similar to that of patients with primary myelofibrosis ( between 60 and 80  years ) . each sample was treated individually for the extraction of nucleic acids and the achievement of microarrays . rna was isolated using rna extraction protocols ( nucleospin rna ii , macherey - nagel ) on the miltenyi plateform . rna samples were quality - checked via the agilent 2100 bioanalyzer ( agilent technologies ) . total rna sample ( 1  g ) rna samples were amplified and labeled using the agilent quick amp labeling kit / low rna input linear amp kit ( agilent technologies ) . the hybridization procedure was performed using agilent gene expression hybridization kit ( agilent technologies ) . briefly , 1.65  g cy3-labeled fragmented crna in hybridization buffer was hybridized overnight ( 17  h , 65  c ) to agilent whole human genome oligo microarrays 4    44k using agilent 's in hybridization chamber . the fluorescence signals were detected using agilent 's microarray scanner system ( agilent technologies ) . the agilent feature extraction software ( fes ) v9.1 was used to read out and process the microarray image files . the signal intensities from single experiment raw data lists are normalized by dividing the intensities values by their median . normalized data were accessible on public database : ( geo submission number gse44426 , http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse44426 ) , online on jan . 10 , 2015 ) , .