malaria occurs often in poor , tropical and subtropical areas and still took an estimated 627 000 lives in 2012 , mostly those of children under five years of age in africa . the culicidae mosquitoes are responsible for transmission of the worm parasites of heart dogs such as dirofileria immitis , dirofileria repense , wuchereria bancrofti , some arboviral diseases such as japanese encephalitis , rift valley fever , western equine encephalitis and eastern equine encephalitis , tahyna , sagiyama , trivitatus , lymphocytic choriomanangitis , west nile virus , st . about 70 anopheles species in the world are able to transmit malaria and of these 40 species have been identified as the major vectors ( azari - hamidian and harbach 2009 ) . by now , 64 species of culicidae mosquitoes due to 3 sub genera and 7 genera are reported from iran ( azari - hamidian and harbach 2009 ) . now , seven species of anopheles reported as the malaria vectors in the country including : an . 2004c , vatandoost and borhani 2004a , vatandoost and moinvaziri 2004b , vatandoost et al . 2005b , sedaghat and harback 2005 , hanafi - bojad et al . 2006a , 2006b , 2007 , 2008 , 2009a , 2009b , moosa - kazemi et al . pulcherrimus as secondary vectors of malaria in the south east of iran ( zaim et al . 1993 ) . twelve insecticides recommended by who for irs currently , which belong to four chemical groups including one organochlorine , six pyrethroids , three organophosphates and two carbamates ( pluess et al . 2010 ) . stephensi was reported in iran in 1957 , subsequently to dieldrin in 1960 , and then to malathion in 1976 ( eshghy 1978 , manouchehri et al . release of larvivorous fish , using the bacillus thuringiensis , and larviciding by chlorpyrifos - methyl are the main larval control measures and pyrethroid as new insecticides are being used as irs and llins in iran ( vatandoost and hanafi bojd 2005a , moosa - kazemi et al . 2007 ) . in spite of more than 50 years malaria - control programming , 42%60%of the total malaria cases reported from sistan and baluchistan province . out of 112 malaria cases representing 79 males and 33 females have been reported in chabahar county , southeastern iran in 2010 . out of 38 cases were from bangladesh , pakistan and afghanistan and the rest were the local aborigines ( moh and me 2010 ) . chabahar is commercial and industrial free zone as well as important due to agricultural and husbandry in sistan and baluchistan province in southeastern iran and also the border line of pakistan . there are scatter data about susceptibility level of malaria vectors in iran , the susceptibility level of culicinae mosquitoes in southeastern country had not been reported . therefore , this study was carried out to determine susceptibility level of mosquitoes to monitoring and evaluation of insecticides resistance . this information could provide an essential clue for judicious use of insecticides and will be very useful to health authorities for future planning of vector control in this endemic malarious area . the study was carried out in chabahar city , siatan and baluchistan province , southeastern iran ( 2517gn , 6037ge ) ( fig . 1 ) . the people engaged to agriculture , horticulture , livestock , fishing sailing , and hand crafts including needlework , making carpet and musical instruments . the absolute maximum and average of temperature was reported 36 c and 16.5 c , respectively ( meteorological organization of iran , 2010 ) . chabahar county with 24 , 729 square kilometers ( 19.3% ) comprised 3 cities , 5 distracts and 11 rural districts . the population of chabahar reported 211,081 including 143,535 rural ( 68% ) and 67,546 urban ( 32% ) . naval base near the 22 bahman squar ( 25 18gn,6037ge ) in chabahar city selected as fix station including , 30 households , and 114 population . baluchi - adam village ( 25 47gn , 6116ge ) with 71 households , and 379 populations , and bahoo - kalat village ( 2543gn , 6125ge ) , 198 households and 1064 population selected randomly as fixed stations . sampling methods such as larval collection , hand catch was carried out during january to december 2010 ( who 1975 , who 1975 ) . these studies were conducted once every 15 days and collected mosquitoes were identified by specific systematic keys ( shahgudian 1960 , zaim and cranston 1986 ) . location of study area in southeastern iran , 2010 in each fixed and variable station larvae was collected from january to december 2010 . larval mosquitoes picked up from the water using a dropper , pipette or fine net and inserted into the bulb . the related data such as water temperature , larval type , number and date sampling was recorded . larvae and pupae in holding container filled with water were transferred to the laboratory for rearing . adult mosquitoes live quite well on bowl of sucrose 10% in bottom of the cage . the adults were kept in the temperature ( 2224 c ) , relative humidity ( 6070% ) and 12l-12d photoperiod ( who 1975a , 1975b ) . culicidae mosquitoes were collected from the villages that spraying was not performed before , between 06.30 and 09.30 am . sampling was carried out in each human dwelling , cattle and goat sheds for 15 min using suction tube and torch ( who 1975b ) . the mosquitoes were inserted in the cage as dimensions of 40 40 cm and sent to the laboratory . total of 200 to 250 mosquitoes were entered in each cage and covered with wet towel . the mosquitoes were kept in standard condition ( 2 5c , 75% h ) . impregnated papers with ddt 4 % , malathion 5 % , propoxur 0.1 % , deltamethrin 0.05 % , lambda - cyhalothrin 0.05 % , cyfluthrin 0.15 % , and control papers were supplied by world health organization . the adult susceptibility test kit including holding and exposure tubes , slides , copper and silver rings and related covers were washed with detergent , and then washed three times with tap water to be free of detergents and insecticides . after drying of the pipe holders , standard paper impregnated with insecticides fixed inside the tubes marked with red dot and copper ring . for holding tube used paper control ( no insecticide ) tube with a green sign and silver pipe fixed to the wall ( who 2006 ) . each time 45 mosquito collected and insert to holding tube overall 2025 mosquito were kept into holding tube . the susceptibility tests performed on their standard condition ( 2226 c , 60% h ) . the susceptibility of the wild strain of culicidae mosquitoes was assessed to the insecticides impregnated papers . the mosquitoes were exposed to different insecticides by different interval times and 24 hours recovery period . the following criteria have been used for interpretation and classification of results , based on who recommendations ( african network on vector resistance 2005 ) . susceptibility test carried out with less than 20 mosquitoes per test have not been consideredat least 80 mosquitoes used per test , three resistance classes as defined . resistant when the mortality was lower than 80 % , susceptible when mortality was 98 % or higher and possible resistant or tolerant when mortality was between 97 % and 80 % .three resistance classes as defined when twenty to 79 mosquitoes used per test , susceptible when mortality was 98 % or higher , resistance suspected , when mortality was between 95 % and 97 % , and resistant when the mortality was lower than 95 % . susceptibility test carried out with less than 20 mosquitoes per test have not been considered at least 80 mosquitoes used per test , three resistance classes as defined . resistant when the mortality was lower than 80 % , susceptible when mortality was 98 % or higher and possible resistant or tolerant when mortality was between 97 % and 80 % . three resistance classes as defined when twenty to 79 mosquitoes used per test , susceptible when mortality was 98 % or higher , resistance suspected , when mortality was between 95 % and 97 % , and resistant when the mortality was lower than 95 % . the samples were recorded in the special forms by and the appropriate time of deaths associated with history of collection , relative humidity and temperature ( shahgudian 1960 , zaim and cranston 1986 , azari - hamidian and harbach 2009 ) . results were considered reliable if the control mortality was less than 5 % and rejected if more than 20 % . results were corrected by abbott 's formula when mortality rates of control group were between 5 to 20 % ( abbott 1925 , who 2006 ) . the lt50 and lt90 were calculated for plotting the regression line using microsoft excel software ver . the study was carried out in chabahar city , siatan and baluchistan province , southeastern iran ( 2517gn , 6037ge ) ( fig . 1 ) . the people engaged to agriculture , horticulture , livestock , fishing sailing , and hand crafts including needlework , making carpet and musical instruments . the absolute maximum and average of temperature was reported 36 c and 16.5 c , respectively ( meteorological organization of iran , 2010 ) . chabahar county with 24 , 729 square kilometers ( 19.3% ) comprised 3 cities , 5 distracts and 11 rural districts . the population of chabahar reported 211,081 including 143,535 rural ( 68% ) and 67,546 urban ( 32% ) . naval base near the 22 bahman squar ( 25 18gn,6037ge ) in chabahar city selected as fix station including , 30 households , and 114 population . baluchi - adam village ( 25 47gn , 6116ge ) with 71 households , and 379 populations , and bahoo - kalat village ( 2543gn , 6125ge ) , 198 households and 1064 population selected randomly as fixed stations . sampling methods such as larval collection , hand catch was carried out during january to december 2010 ( who 1975 , who 1975 ) . these studies were conducted once every 15 days and collected mosquitoes were identified by specific systematic keys ( shahgudian 1960 , zaim and cranston 1986 ) . larval mosquitoes picked up from the water using a dropper , pipette or fine net and inserted into the bulb . the related data such as water temperature , larval type , number and date sampling was recorded . larvae and pupae in holding container filled with water were transferred to the laboratory for rearing . adult mosquitoes live quite well on bowl of sucrose 10% in bottom of the cage . the adults were kept in the temperature ( 2224 c ) , relative humidity ( 6070% ) and 12l-12d photoperiod ( who 1975a , 1975b ) . culicidae mosquitoes were collected from the villages that spraying was not performed before , between 06.30 and 09.30 am . sampling was carried out in each human dwelling , cattle and goat sheds for 15 min using suction tube and torch ( who 1975b ) . the mosquitoes were inserted in the cage as dimensions of 40 40 cm and sent to the laboratory . total of 200 to 250 mosquitoes were entered in each cage and covered with wet towel . impregnated papers with ddt 4 % , malathion 5 % , propoxur 0.1 % , deltamethrin 0.05 % , lambda - cyhalothrin 0.05 % , cyfluthrin 0.15 % , and control papers were supplied by world health organization . the adult susceptibility test kit including holding and exposure tubes , slides , copper and silver rings and related covers were washed with detergent , and then washed three times with tap water to be free of detergents and insecticides . after drying of the pipe holders , standard paper impregnated with insecticides fixed inside the tubes marked with red dot and copper ring . for holding tube used paper control ( no insecticide ) tube with a green sign and silver pipe fixed to the wall ( who 2006 ) . each time 45 mosquito collected and insert to holding tube overall 2025 mosquito were kept into holding tube . the susceptibility tests performed on their standard condition ( 2226 c , 60% h ) . the susceptibility of the wild strain of culicidae mosquitoes was assessed to the insecticides impregnated papers . the mosquitoes were exposed to different insecticides by different interval times and 24 hours recovery period . the following criteria have been used for interpretation and classification of results , based on who recommendations ( african network on vector resistance 2005 ) . susceptibility test carried out with less than 20 mosquitoes per test have not been consideredat least 80 mosquitoes used per test , three resistance classes as defined . resistant when the mortality was lower than 80 % , susceptible when mortality was 98 % or higher and possible resistant or tolerant when mortality was between 97 % and 80 % .three resistance classes as defined when twenty to 79 mosquitoes used per test , susceptible when mortality was 98 % or higher , resistance suspected , when mortality was between 95 % and 97 % , and resistant when the mortality was lower than 95 % . susceptibility test carried out with less than 20 mosquitoes per test have not been considered at least 80 mosquitoes used per test , three resistance classes as defined . resistant when the mortality was lower than 80 % , susceptible when mortality was 98 % or higher and possible resistant or tolerant when mortality was between 97 % and 80 % . three resistance classes as defined when twenty to 79 mosquitoes used per test , susceptible when mortality was 98 % or higher , resistance suspected , when mortality was between 95 % and 97 % , and resistant when the mortality was lower than 95 % . the samples were recorded in the special forms by and the appropriate time of deaths associated with history of collection , relative humidity and temperature ( shahgudian 1960 , zaim and cranston 1986 , azari - hamidian and harbach 2009 ) . results were considered reliable if the control mortality was less than 5 % and rejected if more than 20 % . results were corrected by abbott 's formula when mortality rates of control group were between 5 to 20 % ( abbott 1925 , who 2006 ) . data were analyzed by probit analysis ( finney 1971 , 1978 ) . the lt50 and lt90 were calculated for plotting the regression line using microsoft excel software ver . culicifacies , the species composition of adult culicidae mosquitoes collected by different sampling methods in chabahar county , sistan and baluchistan province , southeastern iran , 2010 an . mortality of culicidae mosquitoes exposed to ddt and other insecticides has shown in tables 1 and 2 . this species was quite resistant to ddt while susceptible to deltamethrin and tolerant to other insecticides ( fig . culicifacies is tolerant to ddt , malathion , and propoxure whereas susceptible to delta - methrin , cyfluthrin and lambda - cyhalothrin ( table 2 ) . the lt50 and lt90 values of this species to ddt 4 % were 18.12 and 46.42 minutes ( table 1 , fig . anopheles dthali was tolerant to ddt and propoxur whereas susceptible to deltamethrin , lamda - cyhalotrin , cyfluthrin and malathion ( table 2 , fig . the lt50 and lt90 values to ddt 4 % were 17.86 and 48.42 minutes ( table 1 ) . regression line parameters of various species exposed to ddt 4% in chabahar county , sistan and baluchistan province , southeastern iran , 2010 b se= slope and its standard error . lt50 , 95 % ci= lethal time causing 50 % mortality and its 95 % confidence interval . lt90 , 95 % ci= lethal time causing 90 % mortality and its 95 % confidence interval . susceptibility level of various culicidae mosquitoes exposed to some insecticides in chabahar county , sistan and baluchistan province , southeastern iran , 2010 mortality rate error bar r resistance , s susceptible , t tolerance regression line of adult culicidae mosquitoes exposed to ddt 4 % in chabahar county , sistan and baluchistan province 2010 it is concluded that cx . pipiens is quite resistance to ddt , propoxur , lambda - cyhalothrin , and cyfluthrin whereas susceptible to malathion ( table 2 , fig . caspius found to be resistant to ddt whereas susceptible to other insecticides ( table 2 , fig . the lt50 and lt90 values to ddt 4 % were 44.68 , and 164.01 minutes ( table 1 ) . culiseta longiaerolata observed tolerant to ddt , propoxur , cyfuthrin , lambda - cyhalothrin whereas susceptible to deltamethrin ( table 2 , fig . the lt50 and lt90 values to ddt 4 % were 17.82 and 51.26 minutes ( table 1 ) . in our study , 4 genera and 9 species of mosquito larvae and adults were identified based on morphological characters . culicidae species were belongs to the genus of anopheles , culex , culisita and ochleratatus . the species of cx . ( 2004c ) reported three biological forms of this species including type , intermediate and mysorensis in southern iran . type and intermediate forms cited as vector in urban areas whereas , mysorensis form as vector in rural area ( vatandoost et al . 1976a , 1976b ) and subsequently to dieldrin in 19609 ( mofidi and saminmi 1960 ) and matlathion in 1976 ( eshghy 1978 ) . following the emergence of resistance of anopheles stephensi to ddt , other organophosphours , carbamate and pyrethroid insecticides were used . stephensi to ddt and dieldrin was studied at various parts of iran bordered in persian gulf and oman sea during 198588 . the results revealed that this species was resistant to ddt and it is quite susceptible to dieldrin ( manouchehri et al . , the results showed that this species was resistant to ddt ( eshghy and janbakhsh 1976 ) . stephensi was susceptible to bendiocarb , propoxur , malathion , fenitrothion , deltamethrin , permethrin , cyfluthrin and lambda - cyhalothrin , resistance to ddt and tolerant to dieldrin in hormozgan province , south of iran . stephensi of bandar abbas strain , showed a relatively high adult resistance of 4.66-fold ( lt50= 108min ) to 4 % ddt ( davari et al . 2007 ) . in our study , the lt50 values of ddt 4.0 % against this species were 29.7 minutes , a manifestation of ddt resistance . stephensi , the main malaria vector , resistant to pyrethroid insecticides in southeastern iran ( vatandoost and hanafi - bojd 2012 ) . anopheles culicifacies comprised of five sibling species of a , b , c , d and e in north and south of india reported as a and b , in north east found d associated sympatric with a and b whereas in central area reported all of sibling species ( goswami et al . polytene chromosome examination has been reported only available method that able to differentiate four members of this complex in areas where species e is not prevalent ( goswami et al . species a has been reported in oman and iran ( zaim and javaherian 1991 ) . this species is known as vector of malaria in east afghanistan ( zahar 1990 ) . in our study , in contrast , ddt resistance reported for the first time after residual spraying in iran by vatandoost et al . culicifacies to pyrethroid and irritability to fenitrothion , cyfluthrin , and permethrin while high extremely resistance to ddt . culicifacies in afghanistan , and also resistance to dieldrin , malathion and ddt in india ( goswami et al . 2006 ) . in our study , dthali found tolerant to ddt and propoxur while susceptible to deltamethrin , lambda - cyhalothrin , cyfluthrin and malathion . in contrast , there are also some reports emphasizing to susceptibility levels of this species in different countries ( who 1992 , hanafibojd et al . 2007 ) . in spite , the existence of many reports about to susceptibility of an . dthali to insecticides , resistance reported to malathion , chloropyriphos , bromophos , carbamate in egypt and temephos in jordan ( who 1992 ) . in our research , cx . pipiens larvae with 1073 ( 51.14% ) collection , was found one of the most predominant species . in adult collection , quinquefasciatus found as 4.82 % of total collection gjullin and peters ( 1952 ) cited decrease of susceptibility level of cx . pipiens was quite resistance to ddt , propoxur , lambda - cyhalothrin , and cyfluthrin , whereas tolerant to deltamethrin and susceptible to malathion . in parallel , there are many reports in relation to some organophosphorus and pyrethroid insecticides resistance in tunisia ( ben cheikh et al . 1991 ) , burkina faso ( chandre et al . 1998 ) , saudi arabia ( amin and hemingway 1998 ) and china ( jinfu 1999 ) . lt50 value for propoxur and malathion calculated as 51 , and 31 minutes respectively ( nazari and janbakhsh 2000 ) . the species reported quit susceptible to dieldrin , propoxur and malathion ( nazari and janbakhsh 2000 ) . in our study , cx . pipiens was highly resistance to ddt , propoxur , cyfluthrin and lambda - cyhalothrin as evidenced by the discriminating concentrations of times . the results may be related to the present of the species complex , application of irs and distribution of llins in southern iran . in the present study , oc . longiaerolata quite resistance to ddt and susceptible to other insecticides the lt50 and lt90 values to ddt 4 % were 17.82 and 50.26 minutes . based on the literature , no reports were available on the susceptibility levels of oc . iran is in the malaria elimination stage ( who 2011 ) . by now , irs in human dwellings and animal shelters , space - spraying , personal protection through distribution of llins and curtains ( icns ) , repellents measures used to control of malaria vectors in iran . in addition , some biological and chemical agents against larval and adult stages of culicidae mosquitoes had been evaluated in the laboratory . results obtained from susceptibility tests of the malaria vectors on ddt revealed that only an . stephensi was highly resistance to ddt in chabahar area , precautionary measures should be taken in future vector control operations . moreover , the status of resistance in other locations in malaria endemic area should be investigated . since the country relies on deltamethrin for irs operation , tolerant populations of cx . pipiens implies careful consideration and regular monitoring of susceptibility level of mosquitoes in the future .