white cottony mycelia formed on the stems of the diseased plants , which became tough and formed abundant amounts of brown spherical sclerotia on the surface of the mycelial mat . initially , stem rot disease affected only a small number of garlic plants ; however , the disease gradually spread and caused large - scale damage . the symptoms of the disease began in may , when the canopy of the garlic plant became densely covered , blocking air flow and light . in late may , relatively high air temperatures and frequent rain fall favored disease development . according to farmers who had experience with this disease , the disease becomes troublesome only when environmental conditions favor disease development . stem rot disease of garlic is a soil - borne disease , the inoculum potential of which increases in continuously mono - cropped areas . it is presumed that the debris of infected plants is main inoculum source of the disease . abundant sclerotia are formed on the stems and bulbs of infected plants , and the sclerotia are overwintered and invade the root , crown , and stem when garlic is planted ( fig . the causal organism of garlic stem rot was isolated from the sclerotia formed on the stems of the infected plants . the surfaces of the collected sclerotia were disinfected with a 1% naocl solution for 1 min , rinsed with sterilized water 3 times , and blotted with 5 layers of flame - sterilized filter papers ( 90 mm ) . the surface - sterilized sclerotia were placed on potato dextrose agar ( pda ) medium and incubated at 25 for 4 days . the fresh mycelia grew out from sclerotia on agar surface were cut with spatula and transferred to new pda and incubated at 25. the causal organism was cultivated in pda for 3 wk , and the morphology of the hyphae and clamp connection structure and sclerotia formation were examined under light microscopy ( table 1 ) . the mycelia were white and grew fast and prosperously on the surface of the pda and had a cottony appearance . 1d ) , were tan to brown in color , and were generally spherical , although some were irregularly shaped . after a 4-day incubation period on pda at 25 , a typical clamp connection structure was observed in the hyphae ( fig . the diameter of the hyphae ranged from approximately 4 to 8 m , and the optimal temperature for growth of the fungus was 30. the causal organism of stem rot disease in garlic and the associated symptoms agreed with the findings of kishi , and the mycological characteristics of the fungus agreed with those reported by mordue . the pathogenicity of the fungus was examined in a garlic plant grown in 1/5000a wagner 's pots . three bulbs of health garlic were planted in wagner 's pots ( 10 replication ) in october 2008 . the inoculum of the fungus was prepared by mixing fungal mycelia and soil 5 kg of sandy loam soil was sieved and autoclaved at 121 for 30 min . and repeatedly autoclaved 3 times at 5-day intervals . the mycelial mat of the test fungus grown on pda for 7 days was harvested . the harvested mycelial mat from 30 petri dishes ( 9 cm in diameter ) was mixed thoroughly with 5 kg of sterilized soil in a plastic container ( 56  35  13 cm ) . the mixture containing the mycelial mat and the soil was dried in the shade for 15 days and then powdered . the powdered mixture was preserved in a green house and used as inoculums ; 200 g of soil inoculum was placed on top of the wagner 's pots . after infestation of inoculum , 500 ml of tap water was added to each pot and covered with one layer of newspaper to maintain sufficient soil moisture . the inoculated pots were kept separately in a green house and were observed for disease symptoms . seven days after inoculation , white mycelia on the stems of the garlic and premodia of sclerotia were observed . as time progressed , the premodia of sclerotia developed into typical brownish sclerotia , and the white mycelia on the stem and soil surface had typical stem rot symptoms ( fig . , many diseases caused by sclerotium rolfsii in various crops have been reported [ 3 - 6 ] . the disease described in this article and the mycological characteristics of the causal organism were in line with these reports . on the basis of the results obtained in this study , the author suggested that the disease observed was stem rot of garlic caused by s. rolfsii saccardo . the isolate obtained from garlic in this study was deposited in the korean agricultural culture collection and was assigned the registration number kacc no .