diabetes mellitus ( dm ) is a systemic disease with several major complications affecting both the quality and length of life . the chronic gram - negative infection of periodontal origin may be considered a potential focus of infection that aggravates metabolic control in patients who have diabetes . the multifactorial model for the aetiology of periodontitis proposed by clarke and hirsch incorporates the role of personal , environmental and systemic factors along with the bacteria to explain individuals varying susceptibility to periodontitis . risk factors associated with periodontitis are smoking , diabetes , stress , neutrophil dysfunction , hyperlipidemia , hormonal imbalance , health care , low socio - economic status , poor oral hygiene , imbalanced diet , and alcoholism . strong evidence indicates that pathogenic bacteria or their products stimulate cells such as fibroblasts , keratinocytes , and macrophages , present in the periodontal tissue , to release a number of inflammatory cytokines , such as tumor necrosis factor alpha ( tnf- ) ; prostaglandin e2 ; interleukin il-1 , 6 and 12 ; granulocyte colony - stimulating factor ( g - csf ) ; and chemokines . the elevated serum levels of these important mediators have deleterious effects on glucose and lipid metabolism . saito et al . found that alveolar bone loss was associated with impaired glucose tolerance in japanese men without diabetes . similarly , nibali et al . found slightly , but statistically significant , higher non - fasting glucose levels in periodontitis cases compared to healthy controls . these studies suggest that periodontitis may effect glucose metabolism in general population , albeit to a lesser extent than in adults with diabetes . therefore our objective of this study was to determine the association between chronic periodontitis and hba1c in adults without diabetes and to evaluate differences in hba1c values that were obtained with the lab and the kit . a total of 140 subjects including both chronic periodontitis patients and healthy controls without diabetes were selected for the study from the outpatient department of periodontics , st . inclusion criteria for the study were the participants of 35 years of age and previously had not been diagnosed with diabetes , presence of > 10 natural teeth and who had not received any surgical / nonsurgical periodontal therapy for past 6 months . we excluded subjects with gross oral pathology ( tumors and cyst ) , pregnant women , subjects who had taken antibiotics and analgesics 6 months prior to surgery and subjects with any other systemic disease . one hundred forty non - diabetic subjects were selected after subjecting their random blood ( 90 mg / dl ) and urine sample for biochemical tests ( benedict 's test ) . the study was approved by the ethical committee of the institution and following the selection of subjects , written consents were obtained from the same . group i : chronic periodontitis patients ( cases ) subjects who were diagnosed with periodontitis having at least 10 teeth with probing depth 5 mm and 15% sites with bleeding on probing ( bop ) and clinical attachment loss > 1 mm were selected . group ii : healthy controls included clinically healthy subjects with no history of any known systemic disease and had no history of previous hospitalization and medication with probing depth < 4 mm , bop at < 15% of teeth sites , and no periodontal treatment within previous 6 months . patients smoking habits were recorded , in this study only 12 patients were smokers ( 7 in cases and 5 in controls ) out of 140 ( 8.5% ) hence , no correlation on the effects of smoking could therefore be drawn from this study . all subjects received a clinical periodontal examination by single examiner who recorded all the variables by manual procedure . gingival bleeding index , probing pocket depth ( ppd ) and clinical attachment level ( cal ) . hba1c was measured using a commercially available test kit ( a1cnow+ ) according to the manufacturer 's recommendations and also with a laboratory method ( ion exchange resin method ) using a semi - auto analyzer . hba1c was compared between cases and controls using the t - test ( unadjusted ) and multiple linear regressions ( adjusted ) . one hundred forty non - diabetic subjects were selected after subjecting their random blood ( 90 mg / dl ) and urine sample for biochemical tests ( benedict 's test ) . the study was approved by the ethical committee of the institution and following the selection of subjects , written consents were obtained from the same . group i : chronic periodontitis patients ( cases ) subjects who were diagnosed with periodontitis having at least 10 teeth with probing depth 5 mm and 15% sites with bleeding on probing ( bop ) and clinical attachment loss > 1 mm were selected . group ii : healthy controls included clinically healthy subjects with no history of any known systemic disease and had no history of previous hospitalization and medication with probing depth < 4 mm , bop at < 15% of teeth sites , and no periodontal treatment within previous 6 months . patients smoking habits were recorded , in this study only 12 patients were smokers ( 7 in cases and 5 in controls ) out of 140 ( 8.5% ) hence , no correlation on the effects of smoking could therefore be drawn from this study . all subjects received a clinical periodontal examination by single examiner who recorded all the variables by manual procedure . gingival bleeding index , probing pocket depth ( ppd ) and clinical attachment level ( cal ) . hba1c was measured using a commercially available test kit ( a1cnow+ ) according to the manufacturer 's recommendations and also with a laboratory method ( ion exchange resin method ) using a semi - auto analyzer . hba1c was compared between cases and controls using the t - test ( unadjusted ) and multiple linear regressions ( adjusted ) . a total sample size of 140 was taken , out of which 70 were cases and 70 were controls and data obtained was subjected to statistical analysis . comparison of select characteristics between chronic periodontitis cases and controls table 2 shows the comparison of hba1c values between chronic periodontitis cases and controls ( kit and lab ) . the unadjusted mean hba1c levels with kit in cases were 5.51  0.53 , while in controls they were 5.44  0.27 , but the difference was statistically not significant ( p = 0.38 ) . the unadjusted mean hba1c levels with lab in cases were 5.50  0.74 , while in controls they were 5.48  0.29 . comparison of hba1c values between cases and controls table 3 shows the comparison of hba1c levels obtained with kit and lab . the mean hba1c levels in cases with kit and lab were 5.51  0.53 and 5.50  0.74 , respectively . the mean hba1c values among kit and lab in controls were 5.44  0.27 and 5.48  0.29 , respectively . comparison of hba1c values of kit and lab table 4 shows correlation between age , bmi , hba1c kit and lab , ppd  5 mm , cal and bop among cases . age showed a positive correlation with bmi ( 0.15 ) , hba1c kit ( 0.03 ) and lab ( 0.11 ) , ppd  5 mm ( 0.20 ) , cal ( 0.26 ) and a negative correlation with bop ( -0.15 ) ; with cal being statistically significant ( p < 0.05 ) . when bmi was compared a positive correlation was found with kit ( 0.17 ) , lab ( 0.13 ) , ppd 5 mm ( 0.30 ) , cal ( 0.22 ) and a negative correlation was found with bop ( -0.14 ) , however ppd  5 mm found to be statistically significant ( p < 0.05 ) . correlation between different variables in cases hba1c kit was positively correlated with lab ( 0.93 ) and was statistically significant ( p < 0.05 ) . a negative correlation was seen when kit was compared with ppd  5 mm ( -0.04 ) and cal ( -0.05 ) while it was positively correlated with bop ( 0.16 ) . with hba1c lab being compared , a positive correlation was observed with bop ( 0.09 ) , ppd  5 mm ( 0.01 ) and a negative correlation with cal ( -0.07 ) . although , both cal ( 0.69 ) and bop ( 0.04 ) showed a positive correlation with ppd  5 mm , only cal was found to be statistically significant ( p < 0.05 ) . table 5 shows correlation between age , bmi , hba1c kit and lab in control group . when age was compared to other parameters , it was found to be positively correlated with same [ bmi ( 0.07 ) , hba1c kit ( 0.17 ) and lab ( 0.05 ) ] . when hba1c kit was compared to lab ( 0.70 ) they were positively correlated and statistically significant ( p < 0.05 ) . periodontitis is defined as  an inflammatory disease of the supporting tissues of the teeth caused by specific microorganisms or groups of specific microorganisms , resulting in progressive destruction of periodontal ligament and alveolar bone with pocket formation , recession or bot . although the biologic mechanisms linking periodontitis to impaired glucose metabolism have not been fully elucidated , a popular theory is that inflammatory mediators ( particularly il-6 and tnf- ) generated within the inflamed periodontal tissues or in response to oral bacteria that translocate into the systemic circulation interfere with the actions of insulin receptors , thereby decreasing insulin sensititvity . glucose can bind irreversibly to hemoglobin through a non - enzymatic reaction to form glycosylated hemoglobin ( hba ) . hba1c is considered as a beneficial indicator of long - term homeostasis , reflecting an average blood glucose concentration for the past 2 - 3 months . diabetes treatment guidelines are based primarily on the establishment and maintenance of hba1c levels < 7% . every percentage point drop in hba1c this study was intended to determine the association between chronic periodontitis and hba1c in adults without diabetes and also to evaluate the difference in hba1c values that were obtained with lab and kit . in this study , cases and controls were of similar age . when relationship between age and hba1c was explored separately in cases and controls , each additional year of age cases had higher bmi scores than controls and after adjustment of bmi to hba1c ( kit and lab ) a positive correlation was found . in this study , hba1c levels ( kit and lab ) were positively correlated with cal and bop also showed positive correlation with hba1c . in this case - control study , hba1c levels were slightly higher and statistically significant in chronic periodontitis cases than in healthy controls ( kit - p = 0.048 ; lab - p = 0.043 ) . in this study , hba1c was measured using a chair side test kit and laboratory method . the hba1c levels that were obtained with chair side kit correlated well with laboratory values . one advantage of chair side hba1c tests is that they can be performed quickly and easily in a dental office setting . further studies are needed to determine whether the screening in the dental office can improve early diagnosis and the management of diabetes . the results of this study are consistent with the study conducted by nibali et al . , which suggested that chronic periodontitis is associated with elevated blood glucose levels in adults who have not been diagnosed with diabetes . in this study non - fasting blood glucose levels was compared between periodontally healthy patients and those with advanced disease . glucose was significantly higher in chronic periodontitis cases than in controls . in this study , in contrast we found a small , but negative correlation ( with lab ) and positive correlation ( with kit ) , between hba1c and number of sites with pd  5 mm . nonetheless , an evidence of dose - dependent relationship between disease extent and hba1c elevations was not found , which suggests that there may be a threshold above which periodontitis affects hba1c values in the general population . a number of participants in this study had elevated hba1c readings and may have had undiagnosed type-2 diabetes . although the usefulness of hba1c as a diagnostic tool for diabetes continues to be debated , several groups have proposed cutoffs ranging from 5.8% to 6.2% . the data from this study suggests that prospective large studies with full measurements of pocket depth and attachment loss , and intervention by periodontal treatment , are needed to clarify the causal relationship between periodontitis and hba1c in non - diabetics . hba1c levels ( lab and kit ) were slightly elevated in chronic periodontitis cases than in controls . this preliminary finding is consistent with earlier reports that chronic periodontitis is associated with elevated blood glucose in adults without diabetes and may increase one 's risk for type-2 diabetes . further studies with larger sample sizes are needed to clarify the causal relationship between chronic periodontitis and glycosylated hemoglobin levels in non - diabetics .