synoptic reporting of all tumor excisions is required by the college of american pathologists ( cap ) . the cap specifically requires that each element in a synoptic report be reported in a required data element ( rde ) pair consisting of the element and the corresponding response ( cap laboratory accreditation process checklist question anp . however , recent studies suggest that specific formatting features are associated with not only the accuracy of the information entered into the report , but also user preference , accuracy of information retrieval , and speed of information retrieval . currently , cap requires that each element of the gleason grading system ( primary pattern , secondary pattern , tertiary pattern , and total score ) be reported on separate lines as separate and distinct rdes , despite the fact that in the biopsy setting these elements are reported on one line as primary pattern + secondary pattern = total score with tertiary pattern on a separate line if and only if it is present . whether these or other formats might affect the reader 's experience is not known . we sought to determine if such differences in reporting formats would affect the speed or accuracy of information retrieval by nonpathologists reading synoptic reports of prostatectomy specimens . to test the accuracy and speed of identification of specific data elements in a synoptic report , a python script was written that provided instructions and a test platform for these quizzes . specifically , the participant is shown a specific phrase that may or may not be in a synoptic report ( with exceptions listed below ) . when the user presses enter the synoptic report appears on the screen and the timer starts . the user then examines the report to determine if the phrase is or is not present . if it is present , they enter the number two , if it is not they enter 1 and then press return . the program automatically records the time and whether the answer was correct , and this data is then transferred to a comma separated values file for further analysis . we constructed our synoptic report for a radical prostatectomy using the checklist from cap , [ figure 1 ] for the standard format . all elements were identical except for changes in the reporting of gleason grade ( primary grade , secondary grade , tertiary grade , and total score ) . the formats were tested in three different quizzes , each given in sequence and at the same sitting . the first quiz contained 36 total questions and compared the standard format [ figure 1 ] with a format in which if there was no tertiary grade the line concerning tertiary grade was omitted [ figure 2 ] . in the standard format , the response for tertiary grade could be 3 , 4 , 5 , or  not applicable . , where ? could be 3 , 4 , 5 , or  not applicable . standard format synoptic report tertiary grade omitted because it was not applicable / present quiz two contained 32 questions and compared the standard format [ figure 1 ] with a biopsy format , [ figure 3 ] , where gleason grades are reported as primary pattern + secondary pattern = total score on one line and tertiary pattern is on a separate line . although this format is taken from and labeled as biopsy format ,  for this quiz , the total score was calculated as it is for prostatectomies where primary plus secondary regardless of the tertiary pattern ( also regardless of whether it was < 5% or > 5% of tissue ) which was reported separately . the question for this part could pertain to any of the 4 elements ( primary pattern , secondary pattern , total score , and tertiary pattern ) , and the answers could be any of 3,4,5,6,7,8,9 , or 10 (  not applicable  was not included ) . in order for the participant to know which format to expect , 16 questions with the standard format were presented and then 16 questions with the biopsy format . within each group , the order of questions were randomized for each participant , but each participant got the exact same questions for each group . biopsy format synoptic report quiz three contained 32 questions and compared the standard format [ figure 1 ] with a biopsy format , [ figure 4 ] , where gleason grades are reported as primary pattern + secondary pattern + ( tertiary pattern ) - > total score on one line . the question for this part could pertain to any of the four elements ( primary pattern , secondary pattern , total score , and tertiary pattern ) , and the answers could be any of 3,4,5,6,7,8,9 , or 10 (  not applicable  was not included ) . in order for the participant to know which format to expect , 16 questions with the standard format were presented and then 16 questions with the biopsy format . within each group , the order of questions were randomized for each participant , but each participant got the exact same questions for each group . they were all nonpathologists and included , five cancer registrars , four mds ( all internists ) , four non - md medical personnel ( all laboratory technologists ) , and three nonmedical personnel ( administrative assistants , other professionals ) . we specifically excluded pathologists from this testing , since we wanted to measure the performance of a user other than a pathologist . similarly , although we have no evidence that different types of readers perform differently on these tests than any other type of reader , like pathologists we did not include urologists to ensure that their particular set of knowledge and experience did not influence the results . although , previous studies have suggested that in this type of test format there were no significant differences between these different users in terms of accuracy or time to retrieval of information , this was again tested in the current study . previously we had noted that participants got faster from quiz 1 to quizzes 2 and 3 as they got practice with the test . as a result , no comparison was made between the three quizzes , and all three quizzes were always taken in the same order . in addition , there was a wide range of speed for the different users . in order to allow comparison between these uses , as a result , the normalized time for the standard format was the control with a normalized time of one , and the time for all other formats was in comparison with that time . statistical analysis was performed using a t - test or fishers exact test as appropriate with a significance threshold of 0.05 . as shown in table 1 , the nonmedical group had a significantly higher accuracy rate than the cancer registrars ( p = 0.02 ) . as a result , any significant findings related to accuracy using grouped data also underwent subset analysis . there was no significant difference in normalized times between any of the four user groups , [ table 2 ] . accuracy for different users compared to cancer registrars normalized time for all questions for different users compared to cancer registrars when tertiary grade was omitted whenever the result was  not applicable ,  the accuracy of classification by the readers was significantly lower than when tertiary grade was listed [ 72 vs. 97% , p < 0.001 table 3 ] . 0.001 ) were evaluated as well as when all other users were evaluated ( 70% vs. 97% , p < 0.001 ) . in addition , when tertiary grade was omitted , the time to answer the question increased significantly ( 63% , p < 0.001 ) [ table 4 ] . accuracy for different formats compared to standard format normalized time compared to standard format when the standard format ( all four elements each on a separate line ) was compared with the biopsy format ( primary + secondary = total , with tertiary on a separate line ) , there was no difference in accuracy of data extraction ( 98 vs. 97% , p = 0.56 ) , however , the time needed to extract the data decreased 18% with the biopsy format ( p < 0.001 ) . nevertheless , no user preferred the biopsy format . when the standard format was compared with a single line format ( primary + secondary + ( tertiary ) - > total ) , the accuracy increased from 97% to 100% ( p = 0.02 ) using the single line format . subset analysis showed that the group with the biggest difference were the registrars ( 6/7 errors with the standard format ) . the time to extract the data also decreased 24% ( p < 0.001 ) with the single line format . nine users preferred the standard format and seven the single line format , even though they were more accurate and faster with the single line format . the data in this report suggest three major conclusions : removing  not applicable  elements from a synoptic report can reduce both the accuracy and speed of information retrieval , combining all elements of gleason grading on one line significantly improves the accuracy and speed of information retrieval from synoptic reports , and users preferences may not always correlate with either accuracy or speed . these results were similar across a wide variety of different users , just as they were in our previous studies . . one of the original intents of synoptic reporting was to have one data element per line , making automated data extraction easier . however , as the tools available for data extraction have evolved , and as the field has moved to data extraction before generating the pathology report , this restriction may not be as important as it has been in the past . in particular with gleason grading , the use of  +  and  =  signs makes regular expression extraction of multiple pieces of information from a single line both easy and routine . in this context , other factors , such as end users ability to quickly and correctly extract information from the report may be of increased value . it is not a surprise that removing elements form a synoptic report reduces the speed of identification of such elements . it has previously been documented that having a reproducible format where all elements are always included in exactly the same order has been associated with faster information identification . when not applicable elements are missing , the reader has to first ensure that indeed they are missing before they can then conclude that they are not applicable . however , we were surprised that removing items from the synoptic format also reduced the accuracy of the response . nevertheless , in constructing synoptic reports , there are many opportunities where omitting  not applicable  elements would reduce the length of the report , a feature that has previously been shown to be associated with decreased error in constructing the synoptic report . whether the benefit in terms of error by making the report shorter outweigh the reduced accuracy of interpretation by leaving out this information is not clear at this time . it is also not a surprise that combining all parts of gleason grading into one line is associated with faster information retrieval . however , in the standard format , the information ( response ) in those four separate lines consists only of a number and the label that explains the number ( rde ) is separated from it on the other side of the page . in discussing this with the participants , they felt that the reason that the single line was faster was not just because it was a single line , but also because they could simply look down the right - hand side of the report ( the responses ) to find the grading section without referring to any rde . this was because the format of the grading information had a structure that was easily identifiable , unique from all other responses in the report , and conveyed information without having to refer to the listed rde . thus , having a unique structure to the response and not just the rde is a key part of improving the speed of information retrieval from a synoptic report . this hypothesis is also consistent with our previous studies that examined the effect of different formats . nevertheless , the format of reporting gleason patterns / grades and scores has been the subject of considerable discussion previously . most importantly , the reasons one may prefer one format over another is multifaceted , and the information in the current study is just one aspect of this discussion . the information we present may be of value in future discussions by pathologists and organizations such as the international society of urologic pathologists who make recommendations using all of the different aspects that affect reporting of gleason grading / scoring . at present , though , current recommendations make a distinction between reporting formats for biopsies and prostatectomies . specifically , in the biopsy specimen if the tertiary pattern is high grade then that is reported as the secondary pattern rather than the  true  secondary pattern . this is done to ensure that the presence of this tertiary pattern is not overlooked by either clinicians or other readers , who may simply omit or skip the presence of a high - grade tertiary pattern if it is only reported in a note . while the rationale for this practice is clear , it does bring up two issues . secondary pattern or a high - grade tertiary pattern , this form of reporting is subject to information loss . specifically , if one reads a report with a gleason score of 3 + 5 , one can not tell from the report whether there are only two patterns ( 3 and 5 ) present in this biopsy , or whether this is a biopsy with patterns 3 , 4 and a tertiary pattern 5 . this may in part explain some of the controversy associated with assigning gleason score 3 + 5 cases in the newly proposed gleason groups . more importantly , there is an assumption in this analysis that the only other way to report tertiary grades is as a separate note . as our study clearly shows , this is not true this study uses just one of many possible options for reporting all four elements on one line . while the order is intuitive ( primary secondary tertiary and total in that order ) the signs that are used to distinguish between the different patterns are not and could be changed if different signs were identified that could convey more information than the signs we have used here . obviously using a plus signs and an equal sign implies that the numbers will add up , and in this format that is not true , and is the reason why we put tertiary patterns in parentheses . using a grade of  0  may also be easier to interpret than using the phrase  not applicable  or finally , recent studies have also suggested that the percentage of high - grade tumor should also be reported as this may also be prognostically significant as well as gleason groups . whether and how this information may be incorporated into a single line format is not clear at this time . nevertheless , the need to begin reporting gleason groups is an opportunity to re - address the most effective format for reporting all elements in gleason grading . first data recognition and user preference are just a few elements of the user interaction with a synoptic report . indeed , several of the nonmedical participants noted that while they could perform the study , they really did not understand the meaning of the phrases they were looking for . this in part explains why we were unable to find a consistent difference in the performance between our different user groups  this test measures the users ability to read and identify information , regardless of their understanding of that information . other measures that focused on comprehension would most likely find significant differences between these different types of users . different report formats for gleason grading significantly affect users speed , accuracy , and preference ; users do not always prefer either speed or accuracy . the data and study design we describe may be useful in deciding between different format options .