these surfactant characteristics result from the combination of the polar hydroxyl groups on the concave -face and the methyl groups on the convex -face . bile salts in aqueous solution form aggregates mainly due to hydrophobic attractive interactions of the apolar -faces . it is known that bile salts form micelles at concentrations above the critical micelle concentration ( cmc ) . the more hydrophobic bile salt is , the lower critical micelle concentration it has , due to more stable hydrophobic aggregations . bile salts are known as permeability enhancers to increase drug penetration through various biological membranes by interacting with phospholipids in cell membranes . micelles of bile salts have been investigated in different pharmaceutical formulations where they solubilise poorly soluble drugs [ 10 - 13 ] . bile salts with more hydrophobic micellar core have greater ability to accept hydrophobic pharmaceutical ingredients . solubilisation capacity of the micellar systems can be increased by chemical modification of the bile salt steroid skeleton . another method to improve solubilisation properties of micellar systems is to produce mixed micelles by combining individual surfactants with different aggregation properties ( cmc values ) . furthermore , intermolecular hydrophilic interactions between polar groups in certain bile salt mixed micelles are known to greatly increase stability of these binary systems . in the pharmaceutical area , various nonionic co - surfactants have been used in bile salt - nonionic binary systems due to ability of these mixtures to improve solubilisation of poorly soluble drugs [ 17 - 23 ] . however , in studies of bile salt - anionic binary mixtures , sodium dodecyl sulfate has been predominantly used as an anionic co - surfactant [ 24 - 27 ] . triton x-100 ( trx ) is a nonionic surfactant with a hydrophilic polyethylene oxide group ( on average it has 9.5 oxyethylene groups ( oe ) ) and a hydrophobic group . trx ( p-(1,1,3,3-tetramethylbutyl)phenoxypolyoxyethylene glycol ) differs from conventional nonionic surfactants , having hydrophilic part longer than the hydrophobic region of the molecule . sodium lauryl ether sulfate ( sles ) is an anionic surfactant with one to four hydrophilic oe groups adjacent to the anionic sulfate group . its basic structure is c12h25(och2ch2)xoso3na where x represents the number of oe groups in the chain . for this work , sles having two oe groups was used . to the best of our knowledge , work on physico - chemical characterisation of binary mixtures consisting of bile salt and polyoxyethylene type of co - surfactant has been done only with individual bile salts . however , there is no comparative data on the physico - chemical properties of mixed micelles of trx or sles with different bile salts . according to the authors ' knowledge investigations of sles - bile salt binary systems have not been reported previously . in the present work , we have tested a set of three bile salts ( sodium cholate ( c ) , sodium deoxycholate ( d ) or sodium 7-oxodeoxycholate ( od ) ) to interact with trx or sles . the results of the study have been analysed using clint 's , rubingh 's , and motomura 's theories for mixed binary systems [ 30 - 32 ] . the first aim of this study was to investigate how different combinations of hydroxyl and oxo groups on the concave - face of the bile salt skeleton can influence the interactions with anionic and nonionic co - surfactant having polar oe groups in the hydrophilic region . the second goal was to evaluate how structural differences in selected anionic and nonionic co - surfactant impact micelization behaviour of developed mixed micelles . it is envisioned that insight on the formation of the micellar structures obtained in this study could be used to develop drug delivery formulations with optimized physico - chemical properties . the cmc values of individual bile salts ( d , c , and od ) and sles were acquired through conductivity and surface tension studies . the experimentally obtained cmc values , with standard deviations of three determinations , for individual surfactants ( cmc ) are presented in table 1 and compared to the literature values ( cmc ) [ 33 - 35 ] . experimentally obtained critical micelle concentrations of the individual surfactants compared to the literature values a the values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations cmcthe cmc values obtained from the literature the critical micelle concentrations of the binary mixtures were studied using conductivity and surface tension measurements , at different mole fractions of trx or sles (  ) . prepared mixtures consisted of 0.1 , 0.2 , 0.3 , 0.4 or 0.5 mole fractions of trx or sles (  ) . for the conductivity studies , a break in the conductivity ( specific conductivity ,  ) versus concentration plot indicated the onset of the micellization process , since this break is characteristic of micelle formation and represents the cmc ( figure 1 ) . the cmc values obtained in the conductivity measurements were acquired from the intersection of the straight lines created above and below the cmc . the straight line above the cmc value was determined by linear regression starting at the highest concentration , while the straight line below the cmc value was obtained by linear regression beginning at the lowest concentration of investigated binary mixtures . the regression diagnostic was constantly performed after addition of each subsequent experimental point . if an experimental point was identified as an outlier , by measuring cook 's distance , the regression would be stopped and the straight line would be formed using previously added experimental points ( figure 1 ) . dependence of the specific conductivity on concentration of trx - od (  = 0.5 ) binary system . the discontinuous line represents the gaussian curve obtained by phillips ' method and the arrow denotes the cmc . however , this method for the determination of cmc values is reliable only if there is an abrupt change in conductivity . as seen in figure 1 , the change in conductivity does not show an abrupt alteration and the intersection point is not clearly detectable . since the uncertainty in determination of cmc values is increased , phillips ' method   was applied in order to obtain the cmc values more reliably . in this approach , the cmc is considered as the concentration at which the maximum change in a physical property of the solution ,  , versus concentration curve occurs . ( d3dc3)c  =  0 ;  c  =  cmc phillips ' method was employed as proposed by mosquera et al . the runge - kutta integration was applied and a least squares fitting method , according to levenberg - maquardt , was used . the usage of phillips ' method for determination of cmc values has been reported in previous studies , as well . figure 1 shows the application of phillips ' method . in order to validate the results determined by means of conductivity measurements plots of surface tension (  ) versus concentration of binary system were obtained , where a break point corresponds to the cmc . dependence of surface tension on concentration of trx - od (  = 0.5 ) binary system . the cmc determination error did not exceed 3% . extended t - test ( p < 0.05 ) shows that the mean cmc values determined using conductivity and surface tension measurements belong to the same population . the experimentally obtained cmc values , with standard deviations of three determinations , for the trx - bile salt and sles - bile salt binary mixtures are presented in tables 2 and 3 respectively . comparision of the experimentally obtained critical micelle concentrations of investigated trx - bile salt binary systems a the values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations comparision of the experimentally obtained critical micelle concentrations of investigated sles - bile salt binary systems a values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations as seen in tables 2 and 3 the cmc values obtained in conductivity and surface tension studies are in good agreement . since there is no statistically significant difference between the cmc values determined by means of the two methods , the cmc values acquired by conductivity measurements were used in further calculations . in order to study the influence of the structure of bile salts on the formation of mixed micelles with trx or sles , the physico - chemical parameters of the micellar systems the determined physico - chemical parameters are : the critical micelle concentration of ideal mixtures ( cmc ) , the mole fraction of the more hydrophobic surfactant in the ideal mixed micelle ( x ) , the mole fraction of the more hydrophobic surfactant in the real mixed micelle ( x1 ) and the  parameter . the physico - chemical parameters for the trx - bile salt and sles - bile salt mixed micelles were calculated according to the equations presented in table 4 . physico - chemical parameters of mixed micelles i the mole fraction in the solution of component i , cmci the experimentally obtained cmc of component i. cmc1 the experimentally obtained cmc values of the more hydrophobic surfactant ( trx or sles ) cmc2 the cmc of d , c or od cmcthe experimentally obtained cmc values of investigated binary systems  the mole fraction of the more hydrophobic surfactant in the solution ( trx or sles ) comparison of experimental with ideal parameters and interaction parameter (  ) of the trx - bile salt mixtures cmcthe experimentally obtained cmc values of investigated binary systems , each result is the average of three separate determinations . cmcthe values for the critical micelle concentrations of ideal mixtures , calculated according to clint 's theory of ideal mixtures . x1 mole fractions of the more hydrophobic surfactant in the real mixed micelles , according to rubingh . xmole fractions of the more hydrophobic surfactant in the ideal mixed micelles , according to motomura 's model . comparison of experimental with ideal parameters and interaction parameter (  ) of the sles - bile salt mixtures cmcthe experimentally obtained values , each result is the average of three separate determinations . cmcthe values for the critical micelle concentrations of ideal mixtures , calculated according to clint 's theory of ideal mixtures . x1 mole fractions of the more hydrophobic surfactant in the real mixed micelles , according to rubingh . xmole fractions of the more hydrophobic surfactant in the ideal mixed micelles , according to motomura 's model . the results show deviation of the cmcvalues from those calculated according to clint 's theory indicating nonideal behaviour of examined mixtures and existence of mutual interactions of the components in the micelles . the difference between xand x1 also indicates nonideal behaviour of the mixtures . from tables 5 and 6 , it can be observed that values of the  parameter come out to be negative at all molar ratios of the mixtures . the more negative value of the  interaction parameter indicates stronger synergistic interactions between the components . these values correspond to the deviation between the experimentaly obtained and calculated cmc values and indicate synergism in all investigated trx - bile salt and sles - bile salt mixtures . in table 7 , the average values of the interaction parameter ( av ) for analysed binary systems are presented in order to make the results easily comparable . average  interaction parameters ( av ) of analysed mixed binary systems the average interaction parameter values show that trx generates stronger sinergistic interactions with investigated bile salts than sles . many recent studies have confirmed that synergistic effect generally occurs in anionic - nonionic surfactant mixtures , improving stability of the system [ 41 - 48 ] . in previously investigated sodium deoxycholate - this value is in good agreement with the average value of the interaction parameter reported in our study ( -0.88 ) . it is known that in mixed miceles composed of two anionic amphihpiles , repulsive electrostatic interactions between anionic heads are important source of destabilisation . hence , weak synergistic or antagonistic interactions between individual surfactants are frequently reported in anionic - anionic surfactant mixtures . therefore , weak synergism that has been noticed for anionic - anionic surfactant mixtures in this article is in agreement with previous studies . the results presented in table 7 show that trx and sles generate the weakest synergistic effect with d , while od creates the strongest interaction with investigated co - surfactants . we assume that the hydrophobic regions of trx and sles are incorporated in the micellar core of investigated binary systems . the polar heads of sles and trx are probably located at the surface of the aggregates , interacting with the polar  side of the bile salts , as presented in figure 3 . for trx - od binary system . consequently , the variation in synergism between investigated bile salts and selected co - surfactants can be attributed to the structural differences in the polar part of the steroid skeleton . spatial relation of triton x-100 and steroid skeleton of sodium 7-oxodeoxycholate in the mixed micelles . obviously , the larger number of hydrophilic groups on  side of the steroid ring generates stronger synergism , since c and od have one polar group more than dc ( 7-hydroxyl and 7-oxo group , respectively ) . namely , it is possible that -hydroxyl groups on the steroid skeleton create hydrogen bonds with proton acceptor oe groups of the polar head of trx and sles . it is known that dc lacks 7-hydroxyl group , thus most likely forms fewer hydrogen bonds . stronger synergistic effect of od in all investigated binary systems compared to the same surfactant systems with c is probably the consequence of the alteration in the spatial position of the oxygen atom at c7 of the steroid skeleton . it is known that -axial hydroxyl group at c7 position of the steroid skeleton forms a 90 angle with the median plane of the steroid skeleton ( mpsc ) . oxidation of this group creates -equatorial oxo group which forms a 30 angle with the median plane of the steroid skeleton ( mpsc ) as seen in figure 4 . therefore , the oxygen atom of the c7 oxo group is moved for 60 towards the mpsc i.e. towards the  side of the steroid ring . spatial position modification of the oxygen atom at c7 ( newman projection of sodium 7-oxodeoxycholate ) . we assume that this spatial relation of the oxygen atom enables polar head ( oe groups ) of the co - surfactants to be oriented towards the -axial hydroxyl groups of the od steroid skeleton , since hydrophilic region of the co - surfactants is probably located at the micellar surface . this spatial orientation of trx is probably achieved through the water molecules , as seen in figure 3 . we assume that similar positioning is attained with sles molecules , through the dipole interactions between the oe group distal to sulfate ion and the 7-oxo group of od . according to the results , trx shows stronger synergism with investigated bile salts than sles . apart from difference in hydrophobic region , this variation can be explained due to the smaller number of oe groups in hydrophilic region of sles , forming fewer attractive interactions with the polar part of selected bile salts . additionally , negatively charged sulfate ion of sles creates repulsive interactions with carboxylate anion of selected bile salts , decreasing stability of mixed micelles . the cmc values of individual bile salts ( d , c , and od ) and sles were acquired through conductivity and surface tension studies . the experimentally obtained cmc values , with standard deviations of three determinations , for individual surfactants ( cmc ) are presented in table 1 and compared to the literature values ( cmc ) [ 33 - 35 ] . experimentally obtained critical micelle concentrations of the individual surfactants compared to the literature values a the values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations cmcthe cmc values obtained from the literature the critical micelle concentrations of the binary mixtures were studied using conductivity and surface tension measurements , at different mole fractions of trx or sles (  ) . prepared mixtures consisted of 0.1 , 0.2 , 0.3 , 0.4 or 0.5 mole fractions of trx or sles (  ) . for the conductivity studies , a break in the conductivity ( specific conductivity ,  ) versus concentration plot indicated the onset of the micellization process , since this break is characteristic of micelle formation and represents the cmc ( figure 1 ) . the cmc values obtained in the conductivity measurements were acquired from the intersection of the straight lines created above and below the cmc . the straight line above the cmc value was determined by linear regression starting at the highest concentration , while the straight line below the cmc value was obtained by linear regression beginning at the lowest concentration of investigated binary mixtures . the regression diagnostic was constantly performed after addition of each subsequent experimental point . if an experimental point was identified as an outlier , by measuring cook 's distance , the regression would be stopped and the straight line would be formed using previously added experimental points ( figure 1 ) . dependence of the specific conductivity on concentration of trx - od (  = 0.5 ) binary system . the discontinuous line represents the gaussian curve obtained by phillips ' method and the arrow denotes the cmc . however , this method for the determination of cmc values is reliable only if there is an abrupt change in conductivity . as seen in figure 1 , the change in conductivity does not show an abrupt alteration and the intersection point is not clearly detectable . since the uncertainty in determination of cmc values is increased , phillips ' method   was applied in order to obtain the cmc values more reliably . in this approach , the cmc is considered as the concentration at which the maximum change in a physical property of the solution ,  , versus concentration curve occurs . ( d3dc3)c  =  0 ;  c  =  cmc phillips ' method was employed as proposed by mosquera et al . the runge - kutta integration was applied and a least squares fitting method , according to levenberg - maquardt , was used . the usage of phillips ' method for determination of cmc values has been reported in previous studies , as well . figure 1 shows the application of phillips ' method . in order to validate the results determined by means of conductivity measurements plots of surface tension (  ) versus concentration of binary system were obtained , where a break point corresponds to the cmc . dependence of surface tension on concentration of trx - od (  = 0.5 ) binary system . . extended t - test ( p < 0.05 ) shows that the mean cmc values determined using conductivity and surface tension measurements belong to the same population . the experimentally obtained cmc values , with standard deviations of three determinations , for the trx - bile salt and sles - bile salt binary mixtures are presented in tables 2 and 3 respectively . comparision of the experimentally obtained critical micelle concentrations of investigated trx - bile salt binary systems a the values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations comparision of the experimentally obtained critical micelle concentrations of investigated sles - bile salt binary systems a values obtained from conductivity measurements . cmcthe mean values  standard deviation of three determinations as seen in tables 2 and 3 the cmc values obtained in conductivity and surface tension studies are in good agreement . since there is no statistically significant difference between the cmc values determined by means of the two methods , the cmc values acquired by conductivity measurements were used in further calculations . in order to study the influence of the structure of bile salts on the formation of mixed micelles with trx or sles , the physico - chemical parameters of the micellar systems were calculated using experimentally obtained cmc values . the determined physico - chemical parameters are : the critical micelle concentration of ideal mixtures ( cmc ) , the mole fraction of the more hydrophobic surfactant in the ideal mixed micelle ( x ) , the mole fraction of the more hydrophobic surfactant in the real mixed micelle ( x1 ) and the  parameter . the physico - chemical parameters for the trx - bile salt and sles - bile salt mixed micelles were calculated according to the equations presented in table 4 . physico - chemical parameters of mixed micelles i the mole fraction in the solution of component i , cmci the experimentally obtained cmc of component i. cmc1 the experimentally obtained cmc values of the more hydrophobic surfactant ( trx or sles ) cmc2 the cmc of d , c or od cmcthe experimentally obtained cmc values of investigated binary systems  the mole fraction of the more hydrophobic surfactant in the solution ( trx or sles ) comparison of experimental with ideal parameters and interaction parameter (  ) of the trx - bile salt mixtures cmcthe experimentally obtained cmc values of investigated binary systems , each result is the average of three separate determinations . cmcthe values for the critical micelle concentrations of ideal mixtures , calculated according to clint 's theory of ideal mixtures . x1 mole fractions of the more hydrophobic surfactant in the real mixed micelles , according to rubingh . xmole fractions of the more hydrophobic surfactant in the ideal mixed micelles , according to motomura 's model . comparison of experimental with ideal parameters and interaction parameter (  ) of the sles - bile salt mixtures cmcthe experimentally obtained values , each result is the average of three separate determinations . cmcthe values for the critical micelle concentrations of ideal mixtures , calculated according to clint 's theory of ideal mixtures . x1 mole fractions of the more hydrophobic surfactant in the real mixed micelles , according to rubingh . xmole fractions of the more hydrophobic surfactant in the ideal mixed micelles , according to motomura 's model . the results show deviation of the cmcvalues from those calculated according to clint 's theory indicating nonideal behaviour of examined mixtures and existence of mutual interactions of the components in the micelles . the difference between xand x1 also indicates nonideal behaviour of the mixtures . from tables 5 and 6 , it can be observed that values of the  parameter come out to be negative at all molar ratios of the mixtures . the more negative value of the  interaction parameter indicates stronger synergistic interactions between the components . these values correspond to the deviation between the experimentaly obtained and calculated cmc values and indicate synergism in all investigated trx - bile salt and sles - bile salt mixtures . in table 7 , the average values of the interaction parameter ( av ) for analysed binary systems are presented in order to make the results easily comparable . average  interaction parameters ( av ) of analysed mixed binary systems the average interaction parameter values show that trx generates stronger sinergistic interactions with investigated bile salts than sles . many recent studies have confirmed that synergistic effect generally occurs in anionic - nonionic surfactant mixtures , improving stability of the system [ 41 - 48 ] . in previously investigated sodium deoxycholate - this value is in good agreement with the average value of the interaction parameter reported in our study ( -0.88 ) . it is known that in mixed miceles composed of two anionic amphihpiles , repulsive electrostatic interactions between anionic heads are important source of destabilisation . hence , weak synergistic or antagonistic interactions between individual surfactants are frequently reported in anionic - anionic surfactant mixtures . therefore , weak synergism that has been noticed for anionic - anionic surfactant mixtures in this article is in agreement with previous studies . the results presented in table 7 show that trx and sles generate the weakest synergistic effect with d , while od creates the strongest interaction with investigated co - surfactants . we assume that the hydrophobic regions of trx and sles are incorporated in the micellar core of investigated binary systems . the polar heads of sles and trx are probably located at the surface of the aggregates , interacting with the polar  side of the bile salts , as presented in figure 3 . for trx - od binary system . consequently , the variation in synergism between investigated bile salts and selected co - surfactants can be attributed to the structural differences in the polar part of the steroid skeleton . spatial relation of triton x-100 and steroid skeleton of sodium 7-oxodeoxycholate in the mixed micelles . obviously , the larger number of hydrophilic groups on  side of the steroid ring generates stronger synergism , since c and od have one polar group more than dc ( 7-hydroxyl and 7-oxo group , respectively ) . namely , it is possible that -hydroxyl groups on the steroid skeleton create hydrogen bonds with proton acceptor oe groups of the polar head of trx and sles . it is known that dc lacks 7-hydroxyl group , thus most likely forms fewer hydrogen bonds . stronger synergistic effect of od in all investigated binary systems compared to the same surfactant systems with c is probably the consequence of the alteration in the spatial position of the oxygen atom at c7 of the steroid skeleton . it is known that -axial hydroxyl group at c7 position of the steroid skeleton forms a 90 angle with the median plane of the steroid skeleton ( mpsc ) . oxidation of this group creates -equatorial oxo group which forms a 30 angle with the median plane of the steroid skeleton ( mpsc ) as seen in figure 4 . therefore , the oxygen atom of the c7 oxo group is moved for 60 towards the mpsc i.e. towards the  side of the steroid ring . spatial position modification of the oxygen atom at c7 ( newman projection of sodium 7-oxodeoxycholate ) . we assume that this spatial relation of the oxygen atom enables polar head ( oe groups ) of the co - surfactants to be oriented towards the -axial hydroxyl groups of the od steroid skeleton , since hydrophilic region of the co - surfactants is probably located at the micellar surface . this spatial orientation of trx is probably achieved through the water molecules , as seen in figure 3 . we assume that similar positioning is attained with sles molecules , through the dipole interactions between the oe group distal to sulfate ion and the 7-oxo group of od . according to the results , trx shows stronger synergism with investigated bile salts than sles . apart from difference in hydrophobic region , this variation can be explained due to the smaller number of oe groups in hydrophilic region of sles , forming fewer attractive interactions with the polar part of selected bile salts . additionally , negatively charged sulfate ion of sles creates repulsive interactions with carboxylate anion of selected bile salts , decreasing stability of mixed micelles . we have studied a set of three bile salts ( sodium cholate , sodium deoxycholate or sodium 7-oxodeoxycholate ) to interact with triton x-100 or sodium lauryl ether sulfate , by conductometric and tensiometric method . the results of the study have been analysed using clint 's , rubingh 's , and motomura 's theories for mixed binary systems . the critical micelle concentration of the ideal mixed micelle , the mole fraction of the more hydrophobic surfactant in the ideal mixed micelle , the mole fraction of the more hydrophobic surfactant in the real mixed micelle , and the  interaction parameter of the mixed micelles were calculated using experimental data . based on the calculated values of the  parameter we have concluded that trx and sles generate the weakest interactions with d , while od creates the strongest interaction with investigated co - surfactants . most probably , hydrogen bonds between -hydroxyl groups of steroid skeleton and proton acceptor oe groups of the polar head of selected co - surfactants are formed and contribute to the synergism . since d lacks one polar group compared to c and od , it showed the weakest interactions . we have also noticed that od generates stronger synergism than c in all investigated binary systems . this is probably due to attractive interactions between 7-oxo group of od and the oxygen atom of the phenoxy ring of trx through the water molecules . it is assumed that attractive interaction between 7-keto group of od and of the second oe group distal to sulfate group in sles promotes formation of hydrogen bonds and stabilisation of the binary system . sodium 7-oxodeoxycholate was synthetized by selective oxidation of the 7-hydroxy group of cholic acid , following the tullar procedure . all bile acids were transformed to the sodium salt by known procedure .cholic and deoxycholic acids were purchased from sigma - aldrich ( germany ) . sodium lauryl ether sulfate having two oxyethylen groups was provided by stepan company , usa . surfactant solutions were prepared by dissolving accurately weighed quantities of surfactants in requisite volumes of deionised water . deionised water ( conductivity < 1 s cm , at 25c ) was used for all purposes . the cmc values of pure c , d , od and sles were acquired through conductivity and surface tension measurements . the cmc value of trx was obtained through surface tension studies only , since this surfactant is nonionic amphiphile and does not have influence on electric conductance . the critical micelle concentrations of all investigated binary mixtures ( d - trx , c - trx , od - trx , d - sles , c - sles , and od - sles ) were determined by means of conductometry and tensiometry . conductivity measurements were carried out on aqueous solutions of pure c , d , od and sles . aqueous solutions of mixtures of c , d or od and one of two co - surfactants ( trx or sles ) were also analysed measuring conductivities . the cmc values of the binary systems were obtained for the different molar ratios of the components . the cell containing solutions was immersed in a water bath , controlling the temperature variation at  0.1c . surface tension measurements were carried out on aqueous solution of trx in order to determine its individual cmc . surface tension measurements were carried out on a sigma 703d tensiometer ( finland ) using a du nouy ring method . sodium 7-oxodeoxycholate was synthetized by selective oxidation of the 7-hydroxy group of cholic acid , following the tullar procedure . all bile acids were transformed to the sodium salt by known procedure .cholic and deoxycholic acids were purchased from sigma - aldrich ( germany ) . sodium lauryl ether sulfate having two oxyethylen groups was provided by stepan company , usa . surfactant solutions were prepared by dissolving accurately weighed quantities of surfactants in requisite volumes of deionised water . deionised water ( conductivity < 1 s cm , at 25c ) was used for all purposes . the cmc values of pure c , d , od and sles were acquired through conductivity and surface tension measurements . the cmc value of trx was obtained through surface tension studies only , since this surfactant is nonionic amphiphile and does not have influence on electric conductance . the critical micelle concentrations of all investigated binary mixtures ( d - trx , c - trx , od - trx , d - sles , c - sles , and od - sles ) were determined by means of conductometry and tensiometry . conductivity measurements were carried out on aqueous solutions of pure c , d , od and sles . aqueous solutions of mixtures of c , d or od and one of two co - surfactants ( trx or sles ) were also analysed measuring conductivities . the cmc values of the binary systems were obtained for the different molar ratios of the components . the cell containing solutions was immersed in a water bath , controlling the temperature variation at  0.1c . surface tension measurements were carried out on aqueous solution of trx in order to determine its individual cmc . surface tension measurements were carried out on a sigma 703d tensiometer ( finland ) using a du nouy ring method . the cmc values of pure c , d , od and sles were acquired through conductivity and surface tension measurements . the cmc value of trx was obtained through surface tension studies only , since this surfactant is nonionic amphiphile and does not have influence on electric conductance . the critical micelle concentrations of all investigated binary mixtures ( d - trx , c - trx , od - trx , d - sles , c - sles , and od - sles ) were determined by means of conductometry and tensiometry . conductivity measurements were carried out on aqueous solutions of pure c , d , od and sles . aqueous solutions of mixtures of c , d or od and one of two co - surfactants ( trx or sles ) were also analysed measuring conductivities . the cmc values of the binary systems were obtained for the different molar ratios of the components . the cell containing solutions was immersed in a water bath , controlling the temperature variation at  0.1c . surface tension measurements were carried out on aqueous solution of trx in order to determine its individual cmc . surface tension measurements were carried out on a sigma 703d tensiometer ( finland ) using a du nouy ring method . this work was financially supported by the provincial secretariat for science and technological development , ap vojvodina , republic of serbia , grant no.114 - 451 - 2113/2011 - 01