chip was performed using tagged tap - abp1 and tap - cbh1 strains with an anti - calmodulin binding protein antibody ( millipore ) and a polyclonal serum against the native sap1 protein9 . high throughput sequencing was performed on the illumina g2 genome analyzer , and analyzed for polymorphism detection or statistical analysis of enrichment . 2d gel electrophoresis was performed as described11 , see supplementary information for construction of the episomal system .