impaired glucose tolerance ( igt ) is a less severe form of hyperglycemia that represents an intermediate stage in the development of type 2 diabetes.13 twenty - five percent to 75% of individuals with igt progress to diabetes within a decade of diagnosis.4 in recent years , the numbers of new patients with type 2 diabetes and igt have steadily increased around the world , and it is estimated that over 300 million people will have type 2 diabetes by the year 2025.5 a number of large - scale clinical studies have made it clear that igt is a major risk factor for cardiovascular disease.68 it is also clear that prevention of diabetes before its onset and aggressive treatment of this disease at an early stage is important from the perspective of public health , medical care , and economics.9 in this context , several intervention studies have demonstrated the positive effects of lifestyle modification.914 it was recently reported that a low - carbohydrate diet ( lcd ) is useful for promoting weight loss and improving glycemic control,1524 but it remains unclear whether the lcd can normalize blood glucose in igt patients and prevent progression to type 2 diabetes . against this background , we designed a 7-day in - hospital educational program focused on the lcd for igt . the aim of the present study was to assess the efficacy of this program combined with diabetes education and support at 3-month intervals for normalizing blood glucose in igt patients and preventing progression from igt to type 2 diabetes . the participants in the present study were 72 japanese patients with igt who were enrolled from april 2007march 2013 at japan labour health and welfare organization niigata rosai hospital ( joetsu , japan ) . we screened patients aged 2075 years with a body mass index ( bmi ) < 35 kg / m , fasting glucose < 126 mg / dl , and hemoglobin a1c ( hba1c ) < 7.0% . patients were eligible for this study if they had igt , which was defined as a glucose level of 140199 mg / dl at 2 hours after a 75 g oral glucose load according to the 2003 american diabetes association criteria . in addition , all of the subjects were confirmed to have a serum triglyceride ( tg ) level < 300 mg / dl and a serum low - density lipoprotein ( ldl ) cholesterol level < 200 mg / dl . patients were excluded if they were pregnant or breastfeeding , had a serum creatinine level 2 mg / dl , had liver dysfunction , had gastrointestinal problems that would prevent them from following the prescribed diet , had active cancer , or were participating in another trial . written informed consent was obtained from all patients and the ethics committee of the japan labor health and welfare organization niigata rosai hospital approved this study . we obtained consent for publication of all the materials in this article , including the individual information contained in the tables . eligible participants were assigned to one of two groups , either a group that was hospitalized for 7 days of lcd education ( lcd group ) or a group that was not hospitalized and had no education ( control group ) . the low - carbohydrate , unrestricted calorie diet aimed at a maximum daily intake of 120 g of carbohydrates . the objectives of the 7-day in - hospital educational program were to create motivation , provide information about diabetes and igt , and change the patient s eating habits . lessons were taught by nutritionists , pharmacologists , nurses , and physicians on a one - to - one or group basis . after the hospital stay , we provided individual support sessions every 3 months with nutritionists , nurses , and physicians . in the control group , participants were given general information about diabetes and igt . their physicians also provided diabetes information brochures with general instructions on diet and lifestyle improvement , but there was no individual instruction or formal group counseling . the lcd group was admitted to hospital for 7 days to participate in an educational program . on the first day of the program , a physician gave a lecture about the lcd using slides that lasted for 60 minutes . on the second day , the subjects received individual lessons from nutritionists , pharmacologists , and nurses . after that , they ate the lcd every day and studied the diet using a textbook that was given to them . in compliance with the lcd , rice ( the main dish ) was offered at lunchtime only . the nutritional composition of the food was 30% carbohydrates , 25% protein , and 45% fat . on the fifth day , the subjects completed some tests on the lcd ( 30 questions , true / false test ) , and discussed their diet with nutritionists , nurses , and physicians at a lunch meeting on the lcd . subjects in both groups were asked to attend outpatient guidance sessions every 3 months and were also reviewed by their physicians at 3-month intervals . body weight was measured and blood and urine samples were collected . the general health of each subject was assessed , and the results of the laboratory tests were explained by the physicians , who gave advice about diabetes or igt for 510 minutes at every interview . we emphasized the total carbohydrate intake of less than 120 g per day without restricting total calories or fat . a 75 g oral glucose tolerance test ( ogtt ) , fasting plasma glucose , fasting plasma insulin , serum ldl cholesterol level , serum high - density lipoprotein ( hdl ) cholesterol , and serum tgs were measured at every 3-month visit . all blood samples were sent to the central laboratory at niigata rosai hospital , and aliquots were stored at 70c until analysis . plasma glucose levels were determined by the glucose oxidation method ( chemical reagents and glucose adams ga-1171 analyzer ; arkray , kyoto , japan ) . hba1c was measured by high - performance liquid chromatography ( hplc ; adams a1c ha-8181 ; arkray ) . the value of hba1c ( % ) was estimated as a national glycohemoglobin standardization program ( ngsp ) equivalent value ( % ) derived from the japanese diabetes society ( jds ) value and calculated by the formula hba1c ( % ) = hba1c ( jds ) ( % ) + 0.4%.25 serum ldl cholesterol and serum hdl cholesterol levels were measured by the direct method without deposition ( sekisui medical h-7700 ; hitachi ltd , tokyo , japan ) . serum tgs were measured by the cholesterol oxidation enzyme and enzymatic colorimetry methods ( sekisui medical h-7700 ) . plasma insulin levels were measured with an enzyme immunometric assay ( immulite autoanalyzer ; diagnostic products corporation , los angeles , ca , usa ) , for which the coefficient of variation was 2.5% . intention - to - treat analysis was done on all patients in their assigned groups , regardless of whether they actually performed the specified intervention . results are expressed as the mean  standard deviation . the differences between baseline values and those after 12 months of treatment were evaluated using the paired t - test for continuous variables . differences between the two groups were assessed using the unpaired t - test for continuous variables and by the chi - square test or fisher s exact probability test for categorical variables because the distribution of each variable did not differ significantly between the two groups . in all analyses , p<0.05 was considered significant and analyses were performed with statview software ( v5 ; brainpower inc . , calabasas , ca , usa ) . eligible participants were assigned to one of two groups , either a group that was hospitalized for 7 days of lcd education ( lcd group ) or a group that was not hospitalized and had no education ( control group ) . the low - carbohydrate , unrestricted calorie diet aimed at a maximum daily intake of 120 g of carbohydrates . the objectives of the 7-day in - hospital educational program were to create motivation , provide information about diabetes and igt , and change the patient s eating habits . lessons were taught by nutritionists , pharmacologists , nurses , and physicians on a one - to - one or group basis . after the hospital stay , we provided individual support sessions every 3 months with nutritionists , nurses , and physicians . in the control group , participants were given general information about diabetes and igt . their physicians also provided diabetes information brochures with general instructions on diet and lifestyle improvement , but there was no individual instruction or formal group counseling . the lcd group was admitted to hospital for 7 days to participate in an educational program . on the first day of the program , a physician gave a lecture about the lcd using slides that lasted for 60 minutes . on the second day , the subjects received individual lessons from nutritionists , pharmacologists , and nurses . after that , they ate the lcd every day and studied the diet using a textbook that was given to them . in compliance with the lcd , rice ( the main dish ) was offered at lunchtime only . the nutritional composition of the food was 30% carbohydrates , 25% protein , and 45% fat . on the fifth day , the subjects completed some tests on the lcd ( 30 questions , true / false test ) , and discussed their diet with nutritionists , nurses , and physicians at a lunch meeting on the lcd . subjects in both groups were asked to attend outpatient guidance sessions every 3 months and were also reviewed by their physicians at 3-month intervals . body weight was measured and blood and urine samples were collected . the general health of each subject was assessed , and the results of the laboratory tests were explained by the physicians , who gave advice about diabetes or igt for 510 minutes at every interview . we emphasized the total carbohydrate intake of less than 120 g per day without restricting total calories or fat . a 75 g oral glucose tolerance test ( ogtt ) , fasting plasma glucose , fasting plasma insulin , serum ldl cholesterol level , serum high - density lipoprotein ( hdl ) cholesterol , and serum tgs were measured at every 3-month visit . all blood samples were sent to the central laboratory at niigata rosai hospital , and aliquots were stored at 70c until analysis . plasma glucose levels were determined by the glucose oxidation method ( chemical reagents and glucose adams ga-1171 analyzer ; arkray , kyoto , japan ) . hba1c was measured by high - performance liquid chromatography ( hplc ; adams a1c ha-8181 ; arkray ) . the value of hba1c ( % ) was estimated as a national glycohemoglobin standardization program ( ngsp ) equivalent value ( % ) derived from the japanese diabetes society ( jds ) value and calculated by the formula hba1c ( % ) = hba1c ( jds ) ( % ) + 0.4%.25 serum ldl cholesterol and serum hdl cholesterol levels were measured by the direct method without deposition ( sekisui medical h-7700 ; hitachi ltd , tokyo , japan ) . serum tgs were measured by the cholesterol oxidation enzyme and enzymatic colorimetry methods ( sekisui medical h-7700 ) . plasma insulin levels were measured with an enzyme immunometric assay ( immulite autoanalyzer ; diagnostic products corporation , los angeles , ca , usa ) , for which the coefficient of variation was 2.5% . intention - to - treat analysis was done on all patients in their assigned groups , regardless of whether they actually performed the specified intervention . results are expressed as the mean  standard deviation . the differences between baseline values and those after 12 months of treatment were evaluated using the paired t - test for continuous variables . differences between the two groups were assessed using the unpaired t - test for continuous variables and by the chi - square test or fisher s exact probability test for categorical variables because the distribution of each variable did not differ significantly between the two groups . in all analyses , p<0.05 was considered significant and analyses were performed with statview software ( v5 ; brainpower inc . , from april 2007march 2013 , we assigned 72 participants to the two groups ( 36 to the lcd group and 36 to the control group ) . there were no significant differences in the baseline characteristics between the two groups ( table 1 ) . after 12 months , the 2-hour plasma glucose level of the lcd group showed a significant decrease in comparison with baseline in the ogtt ( p<0.001 ) . on the other hand , the control group showed a significant increase in the 2-hour plasma glucose compared with baseline ( p<0.01 ) . after 12 months , the 2-hour plasma glucose level in the ogtt was significantly lower in the lcd group than in the control group ( p<0.001 ; table 2 and figure 1 ) . in the lcd group , 25 patients achieved resolution of igt with normalization of glucose levels , eleven patients still had igt , and no patient developed type 2 diabetes . in the control group , three patients achieved resolution of igt , 28 patients still had igt , and five patients progressed to type 2 diabetes . the igt normalization rate was significantly higher in the lcd group ( 25/36 , 69.4% ) than in the control group ( 3/36 , 8% ) ( p<0.001 ) , while the incidence of diabetes was significantly lower in the lcd group ( 0/36 , 0% ) than in the control group ( 5/36 , 13.9% ) ( p=0.02 ) . fasting plasma glucose and hba1c both showed a significant decrease compared with baseline in the lcd group ( p<0.01 and p<0.001 , respectively ) . on the other hand , both parameters increased significantly compared with baseline in the control group ( p<0.01 and p<0.001 , respectively ) . changes in fasting plasma glucose and hba1c over the 1-year period were significantly different between the two groups ( p<0.001 ; figure 2a and b ) . after 12 months , the lcd group showed a significant decrease in weight compared with baseline ( p<0.001 ) . in contrast , the body weight of the control group increased significantly compared with baseline ( p<0.01 ) . the weight of the lcd group was significantly lower than that of the control group at 12 months ( p<0.001 ; table 2 and figure 2c ) . at 12 months , both hyperinsulinemia and homeostasis model of assessment of insulin resistance ( homa - ir ) were improved in the lcd group compared with the control group ( p<0.001 ; table 2 ) . in the lcd group , homa - ir showed a significant decrease compared with baseline ( p<0.01 ) , while homa - ir increased significantly relative to baseline in the control group ( p<0.01 ) . the 12-month change in homa - ir was significantly greater in the lcd group than in the control group ( p<0.001 ; figure 2d ) . the serum ldl cholesterol level of the lcd group was slightly decreased ( p=0.55 versus baseline ) and that of the control group was slightly increased at 12 months ( p=0.53 versus baseline ) , but there was no significant difference between the two groups ( p=0.26 ; table 2 and figure 2e ) . after 12 months , the serum hdl cholesterol level of the lcd group showed a significant increase ( p<0.001 ) , while that of the control group decreased significantly ( p<0.001 ) . thus , the 12-month changes of serum hdl cholesterol were significantly different between the two groups ( p<0.001 ; table 2 and figure 2f ) . after 12 months , serum tgs significantly decreased in the lcd group ( p<0.01 ) , and significantly increased in the control group ( p<0.01 ) . thus , the 12-month changes of tgs were significantly different between the two groups ( p<0.001 ; table 2 and figure 2 g ) . from april 2007march 2013 , we assigned 72 participants to the two groups ( 36 to the lcd group and 36 to the control group ) . there were no significant differences in the baseline characteristics between the two groups ( table 1 ) . after 12 months , the 2-hour plasma glucose level of the lcd group showed a significant decrease in comparison with baseline in the ogtt ( p<0.001 ) . on the other hand , the control group showed a significant increase in the 2-hour plasma glucose compared with baseline ( p<0.01 ) . after 12 months , the 2-hour plasma glucose level in the ogtt was significantly lower in the lcd group than in the control group ( p<0.001 ; table 2 and figure 1 ) . in the lcd group , 25 patients achieved resolution of igt with normalization of glucose levels , eleven patients still had igt , and no patient developed type 2 diabetes . in the control group , three patients achieved resolution of igt , 28 patients still had igt , and five patients progressed to type 2 diabetes . the igt normalization rate was significantly higher in the lcd group ( 25/36 , 69.4% ) than in the control group ( 3/36 , 8% ) ( p<0.001 ) , while the incidence of diabetes was significantly lower in the lcd group ( 0/36 , 0% ) than in the control group ( 5/36 , 13.9% ) ( p=0.02 ) . fasting plasma glucose and hba1c both showed a significant decrease compared with baseline in the lcd group ( p<0.01 and p<0.001 , respectively ) . on the other hand , both parameters increased significantly compared with baseline in the control group ( p<0.01 and p<0.001 , respectively ) . changes in fasting plasma glucose and hba1c over the 1-year period were significantly different between the two groups ( p<0.001 ; figure 2a and b ) . after 12 months , the lcd group showed a significant decrease in weight compared with baseline ( p<0.001 ) . in contrast , the body weight of the control group increased significantly compared with baseline ( p<0.01 ) . the weight of the lcd group was significantly lower than that of the control group at 12 months ( p<0.001 ; table 2 and figure 2c ) . at 12 months , both hyperinsulinemia and homeostasis model of assessment of insulin resistance ( homa - ir ) were improved in the lcd group compared with the control group ( p<0.001 ; table 2 ) . in the lcd group , homa - ir showed a significant decrease compared with baseline ( p<0.01 ) , while homa - ir increased significantly relative to baseline in the control group ( p<0.01 ) . the 12-month change in homa - ir was significantly greater in the lcd group than in the control group ( p<0.001 ; figure 2d ) . the serum ldl cholesterol level of the lcd group was slightly decreased ( p=0.55 versus baseline ) and that of the control group was slightly increased at 12 months ( p=0.53 versus baseline ) , but there was no significant difference between the two groups ( p=0.26 ; table 2 and figure 2e ) . after 12 months , the serum hdl cholesterol level of the lcd group showed a significant increase ( p<0.001 ) , while that of the control group decreased significantly ( p<0.001 ) . thus , the 12-month changes of serum hdl cholesterol were significantly different between the two groups ( p<0.001 ; table 2 and figure 2f ) . after 12 months , serum tgs significantly decreased in the lcd group ( p<0.01 ) , and significantly increased in the control group ( p<0.01 ) . thus , the 12-month changes of tgs were significantly different between the two groups ( p<0.001 ; table 2 and figure 2 g ) . the present study demonstrated that a 7-day in - hospital educational program focused on a lcd is effective for blood glucose normalization in igt patients and for preventing progression from igt to type 2 diabetes . the blood glucose normalization rate was 69.4% in the lcd group , which is higher than previously reported with a low - fat diet ( lfd ) ( 26%47%).12,26 in the ogtt , the 2-hour plasma glucose level was reduced by 33 mg / dl in the lcd group , which was greater than the decrease in previous lfd studies ( 2229 mg / dl).12,27 the 1-year incidence of diabetes among igt patients in the control group was 13.9% , which was within the range suggested by epidemiological data ( 1.5%15.7%).11,2630 on the other hand , the 1-year incidence of diabetes was 0% in the lcd group , which was significantly lower than in the control group ( p=0.02 ) or in previous lfd studies ( 2.0%12.5%).10,26,28 although we did not assess the long - term effects of the lcd , we consider that this diet reduces progression to diabetes in the igt population more effectively than a lfd . first , it is likely that participants with igt have glucose intolerance due to excessive carbohydrate intake ; hence , restriction of their carbohydrate intake leads to blood glucose normalization . in fact , the blood glucose level of most subjects in the lcd group decreased after a few days of hospitalization in comparison with baseline . second , because the lcd does not regulate the total calorie intake and allows patients to eat enough of various foods , it seems that the lcd is easier to understand and conform to than the lfd . third , several studies have indicated that the lcd is more effective for achieving weight loss than the lfd.1524 the lcd group achieved a mean weight loss of 10.6% at 12 months , which was within the range suggested by previous reports on low - carbohydrate therapies ( 3.4%10.9%).1820 this marked weight reduction with the lcd may have led to blood glucose normalization in the lcd group and prevented progression to type 2 diabetes . moreover , homa - ir was significantly lower at 12 months compared with baseline in the lcd group . weight reduction probably contributed to the improvement of insulin resistance . in this study , we observed a significant increase of hdl cholesterol and a significant decrease of tgs ; however , there was no significant alteration of the ldl cholesterol level . recent studies have shown that the lcd is better for achieving weight loss , decreasing tgs , and increasing hdl cholesterol , while the lfd is better for decreasing total cholesterol and ldl cholesterol levels.24 we obtained similar results , which suggests that the lcd might have a weak effect on reducing ldl cholesterol . we designed the 7-day in - hospital educational program so that it focused on the lcd . subjects in the lcd group could study the lcd , actually eat the diet , and change their eating habits during a 7-day hospital stay . on the last hospital day , most subjects already displayed weight loss and a decrease in glucose , and they were praised for achieving this goal , which might have increased their confidence and motivation . the lcd group also received 10- to 15-minute individual counseling sessions every 3 months , and this level of support was sufficient for maintaining their knowledge and motivation . first , it was a case  control study and we could not determine whether both groups had the same unknown bias that affected glucose tolerance . therefore , a large - scale trial would be needed to confirm the generality of our findings . third , we did not have a lfd group as one of the study groups ; hence , we could not assess the superiority of the lcd over the lfd . our study demonstrated that a 7-day lcd education program and periodic interval follow - up are effective for achieving blood glucose normalization and preventing progression to type 2 diabetes in patients with igt . the lcd significantly decreased the 2-hour plasma glucose in the ogtt , as well as fasting plasma glucose , hba1c , homa - ir , body weight , and serum tgs at 12 months , while significantly increasing the serum hdl cholesterol level . however , a large - scale study is necessary to confirm the efficacy of the lcd for igt .