the online version of this article ( doi:10.1007/s11032 - 015 - 0192-x ) contains supplementary material , which is available to authorized users . supplemental table  1 primer pairs used in this study ( xls 32 kb ) 
 supplemental fig .  1 graphical genotype of a bc2f5 line ( hk114 ) used for substitution mapping of rbg2 . positions of marker loci used for genotyping are shown as horizontal lines and were obtained from the linkage map of bils derived from a cross between kele and hitomebore ( mizobuchi et al . the arrowhead shows rm11727 , the nearest marker detected by qtl analysis of an f2 population derived from hitomebore  hk19 ( described in fig .  2 ) . white boxes indicate regions homozygous for hitomebore marker alleles , black boxes indicate regions homozygous for kele marker alleles , and the gray box indicates a heterozygous region .