for the self - assembling process , a solution containing 10 nm scaffold strand , 50 nm of each staple strand ( reverse - phase cartridge purified , bioneer inc . ) , 5 mm tris + 1 mm edta ( ph 7.9 at 20 c ) , 16 mm mgcl2 was heated to 80 c for 4 min , cooled down to 60c over the course of 80 min , and cooled further down to 24 c over the course of 72 h. the folded objects were electrophoresed on a 1.5% agarose gel containing 45 mm tris borate + 1 mm edta and 11 mm mgcl2 at 70 v for 3 h. to prevent the origami structures from denaturation during electrophoresis , the gel - box was cooled in an ice - water bath . the gel - band containing the structures was physically extracted from the gel and run through spin columns ( freezensqueeze spin columns , biorad ) at 5000 rcf . the dna tensegrity objects were then imaged with tem after negative staining with uranyl formate on a fei tecnai t12 biotwin at 80 kv . a 12-helix  bundle kite with four springs of equal length ( 300 nt ) was designed such that the restriction site for ecori is located in one of the 300-nt  spring regions . the assembled kites with a double - stranded region at the ecorri restriction site ( gaattc + 10 bases or more overhang on both sides of the sequence ) were incubated for one hour with the enzymes ( 20 l of 10 nm scaffold , 80 nm each staple , 1xneb - buffer 2 , 20 units of enzyme , new england biolabs , inc . ) . uncut and cut tensegrity kites were electrophoresed on a 1.5% agarose gel . the gel - bands were physically extracted and imaged with tem after negative staining with uranyl formate . for the self - assembling process , a solution containing 10 nm scaffold strand , 50 nm of each staple strand ( reverse - phase cartridge purified , bioneer inc . ) , 5 mm tris + 1 mm edta ( ph 7.9 at 20 c ) , 16 mm mgcl2 was heated to 80 c for 4 min , cooled down to 60c over the course of 80 min , and cooled further down to 24 c over the course of 72 h. the folded objects were electrophoresed on a 1.5% agarose gel containing 45 mm tris borate + 1 mm edta and 11 mm mgcl2 at 70 v for 3 h. to prevent the origami structures from denaturation during electrophoresis , the gel - box was cooled in an ice - water bath . the gel - band containing the structures was physically extracted from the gel and run through spin columns ( freezensqueeze spin columns , biorad ) at 5000 rcf . the dna tensegrity objects were then imaged with tem after negative staining with uranyl formate on a fei tecnai t12 biotwin at 80 kv . a 12-helix  bundle kite with four springs of equal length ( 300 nt ) was designed such that the restriction site for ecori is located in one of the 300-nt  spring regions . the assembled kites with a double - stranded region at the ecorri restriction site ( gaattc + 10 bases or more overhang on both sides of the sequence ) were incubated for one hour with the enzymes ( 20 l of 10 nm scaffold , 80 nm each staple , 1xneb - buffer 2 , 20 units of enzyme , new england biolabs , inc . ) . the gel - bands were physically extracted and imaged with tem after negative staining with uranyl formate .