obesity , defined as a body mass index ( bmi )  30  kg / m , is a chronic disease with major health and economic implications and is recognized as one of the greatest contributors of excessive morbidity and mortality in the 21st century . approximately 30% of the american population is obese , making it the leading nutritional disorder in our society . this trend has escalated to epidemic proportions with a disproportionate increase in persons with superobesity , defined as those with a bmi  50  kg / m .    in 1991 , the national institutes of health issued a consensus statement concluding that in the morbidly obese , bariatric surgery is the most successful intervention for long - term weight loss . significant weight loss following bariatric surgery reduces the inherent obesity - specific comorbidities , lowers cardiovascular risk , and provides a survival benefit in this escalating patient population [ 3 , 4 ] . weight loss surgery has become increasingly utilized , with greater than 225,000 procedures performed in the united states in 2008 according to the american society of metabolic and bariatric surgery . due to the malabsorption induced by the procedure , in conjunction with a reduced gastric volume and alterations in eating behaviors , there is an increased risk of developing certain mineral and vitamin deficiencies . retinol deficiency is more commonly associated with malabsorptive weight loss surgical interventions , and fewer studies have reported this outcome with roux - en - y gastric bypass surgery [ 6 , 7 ] . routine postoperative laboratory surveillance at our institution identified a direct recurring coupling of deficiencies of serum retinol and prealbumin concentration ; this association has not been previously described in the gastric bypass literature . we performed a retrospective chart review of 122 obese patients ( 96 women , 26 men ) who underwent roux - en - y gastric bypass surgery at william beaumont hospital in royal oak , michigan , usa . fifty - four charts had incomplete data and were excluded from analysis ; the remaining 69 subjects served as our study population . the patient population was preapproved for surgery from a multidisciplinary perspective at the william beaumont hospital weight control center . the surgeries were performed between october 2005 and july 2007 by two affiliated bariatric surgeons . data were obtained from william beaumont hospital 's electronic chart system ( one chart , epic systems corporation ) and operative reports . serum markers of nutrition were assessed including : prealbumin , albumin , total protein , and retinol . roux limb measurements were analyzed as a potential mediator of malabsorption , and liver function studies were obtained . patients were interviewed at baseline and follow - up intervals by our team of bariatric dietitians for nutritional compliance with dietary protein intake . confidentiality was protected by assigning patients ' anonymous numbers , and the study was approved by the hospital 's human investigation committee . our protocol at the william beaumont hospital weight loss center involves regular follow - up outpatient visits at 6 weeks , 3 , 6 , 9 , and 12 months with a multidisciplinary team that includes a dietitian , exercise physiologist , psychologist , and bariatrician .   the dietitian works to optimize dietary intake and assess for food intolerances . routines recommendations include consuming a higher daily intake of lean protein ( approximately 1.2  g / kg of ideal body weight ) , which generally corresponds to 5580 and 70110 grams for women and men , respectively .   other important dietary principles include avoidance of excessive sugars ( > 5 grams per serving ) , which can promote a dumping syndrome , and inclusion of < 30% of daily intake from fat per day to avoid steatorrhea . patients are also counseled to minimize the consumption of partially saturated and hydrogenated fats . we routinely recommend a chewable multivitamin twice daily , calcium citrate 500  mg three times daily with 400  iu of vitamin d , ferrous sulfate 30  mg daily separated by 2 hours from the calcium compound , and 1000  mcg of cyanocobalamin daily to prevent vitamin and mineral deficiencies . laboratory surveillance of commonly reported mineral and vitamin deficiencies as well as protein levels prompted a tiered response of additional treatments as needed . demographics and baseline characteristics are reported as means    standard deviation ( sd ) or counts with percent frequencies as appropriate . spearman correlations were completed between weight change and vitamin a levels with all the continuous variables at both 6 weeks and 1 year . univariate comparisons between patients meeting their protein goal and those that did not were made using either a test for normally distributed data or wilcoxon rank tests for the outliers . categorical variables were examined using pearson 's chi - square as appropriate ( expected frequency > 5 ; otherwise fisher 's exact tests were used ) . weight loss at 6 weeks and 1 year following surgery was 20.1  9.1  kg and 44.1  17.1  kg , respectively ( figure 1(a ) ) .   at 6 weeks and 1 year , 35% and 18% of patients were vitamin a deficient ( < 325  mcg / l ) . similarly , 34% and 19% had low prealbumin levels ( < 18  mg / dl ) at these time intervals ( figure 1(b ) ) . vitamin a directly correlated with prealbumin levels at 6 weeks ( r = 0.67 , p < .001 ) , and 1-year ( r = 0.67 , p < .0001 ) . there was no significant correlation between roux length measurement and serum vitamin a concentrations , at 6 weeks and 1 year ( r = 0.008 and 0.008 , resp . ; p = .96 for both ) .   similarly , the correlations between the roux length and prealbumin concentrations at 6 weeks or 1 year were insignificant ( r = 0.08 ; p = .55 and r = 0.001 ; p = .99 ) .   achieving dietary protein intake goal , defined as average daily dietary protein intake within 5 grams of intake goal or greater on average , was also not associated with serum levels of vitamin a at 6 weeks or 1 year ( p = .41 ; p = .24 , resp . ) . no significant correlations between vitamin a levels and markers of liver function ( aspartate aminotransferase and alanine aminotransferase ) were observed at 6 weeks ( p = .29 ,  .98 , resp . ) or at 1 year ( p = .34 ,  .99 , resp . ) . vitamin k assessments were not included in the study methodology and can not be commented on . we examined the associations between zinc , protein , and vitamin a in various forms and did not identify any significant relationships . vitamin a is an essential fat - soluble vitamin absorbed through the small intestine as either retinol ( animal derived ) or carotene ( plant and vegetable derived ) . subsequently , it is converted to retinyl palmitate and hydrolyzed to bound retinyl binding protein that transports vitamin a to tissues . several potential mechanisms may exacerbate vitamin a deficiency in a postoperative gastric bypass patient . first , the deficiency may arise from surgically bypassing the duodenum and first portion of the jejunum , promoting an iatrogenically induced malabsorption . second , drastic decreases in the dietary intake of many micronutrients like carotenoids and retinol , especially in early recovery , are likely to occur .   in addition , traditional dietary recommendations after gastric bypass include a low - fat diet which potentially limit the absorption of fat - soluble vitamins . this patient subset may also be at risk due to confounding nonalcoholic steatohepatitis , higher rates of cirrhosis , or both , which may interfere with maintaining vitamin a storage and production . finally , higher levels of oxidative stress may also occur after gastric bypass surgery , which can interfere with vitamin a absorption and processing . vitamin a deficiency is rarely described in western society ; however , worldwide , it remains the most common etiology of visual disturbances , including blindness . vitamin a deficiency has been ascribed to a wide variety of ophthalmologic complications including conjunctival and corneal xerosis , keratomalacia , retinopathy , visual loss , and nyctalopia . moreover , retinol supports photosensitive pigmented cells of the retinal rods and cones that are necessary for optimal visual acuity . serum retinol levels and protein - calorie malnutrition have been correlated among children and infants in developing nations . vitamin a deficient children treated with an augmented dietary protein intake demonstrated an increase in serum protein markers as well as serum retinol levels . in this extreme clinical scenario , retinol deficiency was successfully managed through this dietary intervention alone , without the addition of vitamin a rich foods or vitamin a supplementation . the present findings support the intimate interaction that serum retinol levels have to carrier proteins which determine the bioavailability of serum retinol concentration and reinforce the interdependent relationship of these nutritional markers .   as such , total body stores of vitamin a may not be truly deficient ; in reality , limited access to nutritional protein binding and transport capacity may underlie these serum retinol findings . these data suggest that addressing the nutritional protein levels is necessary in conjunction with deficient serum retinol concentrations . there are several case series describing vitamin a deficiency with visual disturbances involving patients who have had gastric or intestinal surgery [ 6 , 7 , 10 ] . none of our patients complained of visual disturbance ; however , ocular complaints may have been underreported in the scope of this paper because many clinical features especially early in the course of retinol deficiency can be vague or nonspecific and may not have been recognized as clinically relevant . it is important to acknowledge this potential complication after bariatric surgery in patients who undergo longstanding iatrogenic malabsorption with limited nutritional protein stores   and consider appropriate diagnostic testing and referral for ophthalmologic assessment , when appropriate . accordingly , our population was limited to those patients who were compliant with their baseline evaluation , 1-year follow - up exam and serial laboratory testing . subjects with missing preoperative or postoperative lab values were excluded from the analysis , which may have biased our study in representing a more compliant subset of patients .    because we captured these data in the scope of clinical management , multivitamin brands and additional retinol supplementation may have varied according to patient preference and our treatment methodology . we also did not account for other potential confounding variables , including physical activity , nutritional compliance , or the duration of supplementation . nevertheless , vitamin a deficiency is of escalating interest in clinical centers and will likely be the focus of future research . we noted a striking , direct relationship between postoperative nutritional protein levels and vitamin a concentrations in our gastric bypass populations . vitamin a deficiency is common after gastric bypass and is directly associated with a low serum prealbumin concentration , a measure of protein - calorie malnutrition . this fat - soluble vitamin deficiency should be considered in postoperative patients and deficiency should be strongly suspected in those with evidence of protein calorie malnutrition . improving awareness and understanding of total body vitamin a utilization is of paramount importance in the ongoing medical management of this at - risk population .