secondary hyperparathyroidism ( shpt ) is a major complication of chronic kidney disease ( ckd ) , resulting from disturbances in the regulation of parathyroid hormone ( pth ) , calcium , phosphorus , and vitamin d. although hyperphosphatemia appears to be particularly important in the development of shpt , the complication often occurs early in stage 3 of kidney failure , before the development of hyperphosphatemia . several factors , including pth and vitamin d , play a criticalrole in this system . fibroblast growth factor23 ( fgf23 ) , a recently identified phosphatonin , is also implicated . although , a significant role of fgf23 inphosphate homeostasis in physiological conditions is described in many studies with the kidney functioning normally , few studies are available on end - stage renal disease . one of the most commonly cited morbidity associated with end - stage kidney disease is renal osteodystrophy , secondary to the alterations in mineral metabolism . to understand the interrelatedseries of events leading to that , it is first necessary tostudy the mineral metabolism and its physiological process . the present study aims to evaluate the relation between serum fgf23 , phosphorus , and pth in end - stage renal disease patients with shpt on regular hemodialysis . all patients who had shpt and were on regular hemodialysis in nephrology unit in suez canal university hospital , from january to july 2010 , were included in the study ( case group , comprising 46 patients ) . twenty healthy adults ( with normal kidney function ) matched for age and gender were considered as the control group . serum phosphorus , calcium , ferritin , albumin , hemoglobin level , blood urea , and creatinine were assessed by standard protocols at the department of clinical pathology in suez canal university hospital . intact fgf23 concentrations were measured using human fgf-23 ( c - term ) elisa kit according to manufacturer 's ( immutopics , inc . serum samples were collected from each individual at a single time point and kept at 70c until analysis . before the commencement of the study , informed consent was obtained from all individuals selected for the study . the aim and the value of the work there was no chance of any harm being inflicted on them ; on the contrary , all would have the benefits of follow - up and the results of the study . the study was approved by the ethics committee of the faculty of medicine , suez canal university . the student t test , correlation coefficient , and chi - square tests were used to evaluate the results . chi - square test was used for qualitative variables , while independent t test was used for quantitative variables . before the commencement of the study , informed consent was obtained from all individuals selected for the study . the aim and the value of the work there was no chance of any harm being inflicted on them ; on the contrary , all would have the benefits of follow - up and the results of the study . the study was approved by the ethics committee of the faculty of medicine , suez canal university . the student t test , correlation coefficient , and chi - square tests were used to evaluate the results . chi - square test was used for qualitative variables , while independent t test was used for quantitative variables . all the patients ( 46 patients ) of the case group were on hemodialysis for an average duration of 4.9 years . the age of the patients ranged from 20 to 52 years ( 34.7 + 11.8 ) years . forty - one ( 89.8% ) patients were on regular vitamin d and calcium supplements . the control group consisted of 20 healthy adults with normal kidney function ; their mean age was 30.37.9 years . table 2 shows that fgf23 has a significant positive correlation with serum phosphorus , urea , creatinine , ferritin , hemoglobin , and pth levels ( p < 0.01 ) . table 3 shows that pth has a significant positive correlation with serum phosphorus level ( p < 0.01 ) . the scatter plots show positive correlation between fgf23 and serum phosphorus levels ( r= + 0.9 ; p < 0.001 ) and between fgf23 and serum pth levels ( r= + 0.6 ; p < 0.01 ) [ figure 1 and figure 2 ] . laboratory characteristics of the studied patients in comparison to control correlation between fgf-23 and various parameters correlation between parathyroid hormone level and other parameters correlation between fgf-23 and serum phosphorus level correlation between fgf-23 and parathyroid hormone shpt is an insidious disease that develops early in the course of ckd and increases in severity as the glomerular filtration rate deteriorates . it is not yet proven if there is any direct relationbetweenpth and fgf23 . in our study , levels of both pth and serum phosphorus in the case group , were significantly higher in comparison with those in the control group . as the number of functioning nephrons decreases , the failing kidneys are unable to excrete phosphorus and there is a progressive increase in serum phosphorus levels . the ongoing inability to excrete phosphorus leads to continual over - stimulation of the parathyroid glands , tissue hyperplasia , and over - secretion of pth . the development of shpt in our patients was most likely a sequela of this series of classic events . hemoglobin levels were significantly low in the case group and negatively correlated with both pth and fgf23 levels , which indicates that shpt may have a partial role in the development of anemia in patients with ckd . high pth levels may contribute to anemia by directly inhibiting the production of red blood cells and increasing their fragility . shpt can also cause marrow fibrosis , further decreasing the production of red blood cells . it is worth noting , that a recent prospective study has shown that fgf23 mediates the development of hypophosphatemia after i.v iron poly - maltose administration . in our study , most of the patients received parenteral iron , and further correlation was observed between ferritin and fgf23 blood levels , which warrants further studies to evaluate the mechanism whereby parenteral iron therapy influences fgf23 metabolism and to identify additional systemic factors regulating fgf23 production , in order to enhance our understanding of phosphate homeostasis . in addition , the possible role of fgf23 in the development of anemia is explicable . the degree of fgf23 elevation , seen in the current data , correlates positively with thedegree of hyperphosphatemia . in experimental studies , phosphate loading in mice increasesfgf23 levels . previous studies on dietary phosphate as a regulator of fgf23 showed conflicting results.[1417 ] small sample size , and differences in magnitude or duration of altered phosphate intake may explain the contradictory results . although fgf23 inhibits phosphate re - absorption in the proximaltubule , leading to hypophosphatemia , phosphate levels were significantly high in our case group in spite of the concomitant 4-fold increases in fgf23 levels . the possible explanation is that the kidney , which is the principal target of fgf23,is no longer responsive to fgf23 in ckd . another explanation is that , in early stage of ckd , serum fgf23 is elevated to maintain normal serum phosphate levels by promoting urinary phosphate excretion ; but in patients at the advanced stage , overt phosphate loading may overcome such compensation for decreased glomerular filtration rate ( gfr ) despite markedly elevated fgf23 levels . on the other hand , the precise mechanism and site of definitely increased fgf23 levels havenot been evaluated in the present study . it is possible thatboth increased production and decreased metabolism or clearanceof fgf23 contributes to elevated levels of fgf23 in ckd . althoughno studies have yet documented increased production of fgf23,the correlation of fgf23 levels with increasing blood urea and creatinine in our study and therapid decrease in fgf23 levels in some patients after renaltransplantation in other studies , suggest that fgf23 is cleared by the kidney . previous in vivo studies have shown a stimulatory effect of 1,25(oh)2vit d3 on circulating fgf23 levels in rodents and in humans.[2022 ] it is an interesting point as most of our studied patients received high doses of active vitamin d , which may be implicated in the pathophysiology of the observed rises in fgf23 . however , the function ofhigh fgf23 levels in the control of phosphate levels in ckd is still unexplained , and more studies are necessary . however , there is growing evidence that pth may stimulate fgf23 expression and secretion by bone tissue . in the setting of primary hyperparathyroidism , elevated fgf23 concentrations have been observed by several groups.[2325 ] on the other hand , in the study by hiroyuki et al . , no difference in serum fgf23 levels was found between healthy controls and primary hyperparathyroidism patients with normal renal function ; also , there were no significant relations detected between serum fgf23 levels and the levels of pth . as several agents influence the release of pth and fgf23 , the presence of altered calcium or magnesium levels , subsequent to administration of variable supplements to the patients , confounds the interpretation and conflicts of any association between fgf23 and circulating levels of pth . a strongpositive correlation between elevated fgf23 levels and the severity ofhyperparathyroidism in ckd group ( case group ) was observed in our study . although the mechanism of this finding is unclear , it is possible that chronic phosphate retention as reflected by elevated fgf23 levels may have contributed to further stimulation of pth secretion , progression of parathyroid hyperplasia , and parathyroid cell proliferation . another possibility is that high levels of fgf23 at baseline may be a consequence of prolonged active vitamin d administration for severe hyperparathyroidismin our patients , as mentioned above . therefore , fgf23 might indirectly contribute to the development of shpt associated with renal insufficiency . furtherstudies are required to document the effects of fgf23 on pth production and secretion and on parathyroid cellproliferation and to assess the role of fgf23 estimation in predicting the future development of refractory shptin ckd . the precise role of extremely elevated fgf23 in the control of hyperphosphatemia ; and their direct effect on parathyroid function and development of anemia in ckd patients on hemodialysis still remain unclear . their positive correlation with pth may suggest that fgf23 is a central factor in the early pathogenesis of shpt.using fgf23 as a biomarker for assessing phosphate retention or as a predictor of morbidity and future development of refractory shpt in ckd needs more studies .