philosophical , religious , and cultural differences in the concept of death and ways it is defined make discussions of the subject very complex . advances in medicine and technology that have made it possible to support , repair , or replace failing organs challenge commonly held notions of life and death . the lack of understanding or even awareness among the public   and health professionals   and the emotionally charged nature of the subject further complicate discussions . the growing disparity between the demand for and the availability of transplantable organs is a public health concern globally . in comparison to bridge technologies for end - stage organ failure , such as kidney dialysis   and ventricular assist devices , organ transplantation is life - preserving , life - enhancing , and cost - effective . yet , when the supply of transplantable organs falls short of demand , unethical and illegal behaviours sometimes occur , including commercial organ trade that victimizes the vulnerable , human killing as a source of transplantable organs , and violations of the allocation system . while the public supports organ donation after death , its credibility requires clarity in practice and policy . depending on the country and related professional societies , guidelines may or may not exist for the determination of death by neurological and/or circulatory criteria , and when guidelines are in place , they have not always been implemented in a manner that alleviates concerns about the legitimacy of deceased organ donation . the situation is complicated by variations related to the stage of development of a country s deceased donation program and more generally by the significant diversity in health services and standards of care worldwide . the world health organization ( who ) and the transplantation society ( tts ) have received requests from a number of countries to address these gaps and to provide guidance to inform clinical practices and health policy for death determination as part of an international effort in support of the istanbul declaration , the who madrid resolution , and the who guiding principles on human cell , tissue and organ transplantation , approved by the world health assembly in 2010 . an international consensus on the determination of death could provide a number of benefits including promoting evidence - based practices , protecting the rights of both patients and health care professionals , improving public and professional confidence in the process of deceased donation , and increasing the number of organs obtained in an ethically legitimate fashion . on 3031 may 2012 , an invitational forum sponsored by health canada and canadian blood services in collaboration with the who was held in montreal , canada , as part of the planning , scoping , and needs assessment phase in the process of guideline development ( see  appendix 1  for forum committees and participants ) . the 32 invited participants included delegates from a broad range of national and international professional societies involved with death determination in adults and children in acute care settings . prior to the forum , participants were provided with comprehensive background materials , including a selected bibliography of peer - reviewed articles related to death determination , a literature review on definitions of death , and a draft lexicon of medical terminology relevant to death determination policy and practice . each section of the meeting began with an expert speaker who provided historical information , reviewed baseline knowledge , and supplied his or her perspective on current issues and controversies . after each presentation , participants asked questions of the expert and discussed the presentation with the entire group . reference sheets that offered condensed summaries of existing evidence were distributed , and the participants were then divided into groups where they held extensive discussions about a  challenge question  relevant to the topic of the session . when the participants reconvened in the plenary , each group s results were discussed , and the collective outputs were reworked until consensus was reached . consensus was defined as achieving substantial agreement , manifested by all participants indicating that they agreed with or could accept a conclusion , which they would support both within and outside the meeting . for the purposes of this forum , death was fundamentally considered a biological event . while it was respectfully recognized that legal , ethical , cultural , and religious perspectives on death can impact the utility of recommendations in the field , these perspectives were not topics for debate or discussion at this initial meeting . table  1 highlights the common terminology that was agreed upon to support clarity and precision in the language used throughout this forum and for subsequent phases of work . the planning committee worked with participants to identify critical events that comprise the dying sequence , with the understanding that dying is a process whereby biological / physiological functions cease . death is an event in that dying process , a point when the person can be determined to have died . the forum identified the tests required for the minimum acceptable clinical standard for determining death in adults and children and recommended additional testing beyond the minimum standard . a consensus on an operational definition of human death was then derived.table  1participants agreed to the following terminology , in order to improve the clarity of discussions and debate , for use during and subsequent to this forumtermdefinitionactivityphysiologic properties of cells and groups of cells that can be measured by laboratory meansasystole electricala condition characterized by the absence of electrical , and hence mechanical , activity of the heart , resulting in the absence of contractions of the myocardium and cardiac output / anterograde blood flowasystole mechanicalthe absence of effective contractions of the myocardium and no cardiac output / anterograde blood flow . may occur in the presence of an organized or disorganized electrocardiac rhythm , e.g. pulseless electrical activityautoresuscitationthe spontaneous resumption of heart contractions causing anterograde circulation that is not induced by cardiopulmonary resuscitation or other external assistance . examples of heart function such as effective contractions of the myocardium leading to anterograde flow of blood through the aorta and arterial system should be distinguished from examples of heart activity such as atrial natriuretic hormone release or residual pulseless electrical activitybrain deathdiagnosis and confirmation of death based on the irreversible cessation of functioning of the entire brain , including the brainstem ( this forum supports the movement away from this traditional and imprecise terminology in favour of the cessation of neurological function)brainstem deathdiagnosis and confirmation of death based on the irreversible cessation of functioning of the brainstem , predominantly but not exclusively secondary to a supratentorial brain injury ( this forum supports the movement away from this traditional and imprecise terminology in favour of the cessation of neurological function)cardiac arrestthe abrupt cessation of circulation of the blood due to failure of the heart to contract effectively . also known as cardiorespiratory arrest , cardiopulmonary arrest , or circulatory arrestcatastrophic brain injury leading to deathetiologies of high severity that are common causes of brain death , such as , but not limited to , traumatic brain injury , cerebrovascular accidents , and hypoxic - ischaemic encephalopathy after resuscitated cardiac arrestother forms of catastrophic brain injury that have any degree of residual clinical brain or brainstem function are not under consideration and may include persistent vegetative states , permanent vegetative states , anencephaly , or those conditions related to the historical concept of higher brain ( cortical ) deathcerebral electrical activityelectrical activity of the brain , as measured using an electroencephalogram ( eeg)cessationstoppage , terminationcirculationanterograde flow of blood through the aorta and arterial systemcirculatory death determinationdiagnosis and confirmation of death based on circulatory criteria . also known as death after cardiac arrest , or death after cardiocirculatory arrest , or death after circulatory - respiratory determination ( this forum supports the movement away from this traditional and imprecise terminology in favour of the cessation of circulatory function)clinicalbased on direct , measurable observation or examination of the patientcomaprolonged absence of wakefulness , awareness , and the capacity for sensory perception or responsiveness to the external environmentconfounding conditionscircumstances during which a diagnostic test may become unreliable and require repetition over time or application of an alternative testconsciousness  loss of capacity forlack of current or any future potential for awareness , wakefulness , interaction and capacity for sensory perception of , or responsiveness to the external environmentcriteria the standard recommended by this forum sets the minimum clinical criteriadead donor rulea principle governing deceased donation practices stating that vital organs should only be taken from dead patients and , correlatively , living patients must not be killed by organ retrieval . this rule does not apply to , nor preclude , living donation of non - vital organsdeaththe moment in time during the dying process when the individual passes from the state of being alive to that of being deaddeath concept ofan abstract , unprovable explanation of death , generally based on religious , spiritual , or philosophical beliefsdeath declaration ofthe point in time at which a health professional , having determined that an individual is dead , formally states this findingdeath  operational definition ofbiomedical criteria that describe the state of human deathdeath determination ofprocesses and tests required to diagnose death in accordance with established criteriadisintegrationloss of intactness , solidness , or cohesion . such loss can apply to function or to matter ( tissues , etc.)dyingthe process whereby biological / physiological functions cease , thus moving from the state of being alive to that of being deadecmoextracorporeal membrane oxygenation / extracorporeal oxygenation and circulation of blood deployed for life - threatening lung or heart  lung failureelectromechanical dissociationa form of mechanical asystole . a rhythm frequently encountered during cardiac arrest , characterized by organized electrical activity without circulation , traditionally measured by the absence of a palpable pulse or pulsatile arterial blood pressurefixed dilated pupilspupils in mid - position or greater and unreactive to lightfunctionin the context of organs , the primary and fundamental purpose of that organ that can be assessed by observation and examination and is necessary for sustained life . function should be distinguished from activities , as defined by physiologic properties of cells and groups of cells that can be measured by laboratory means . examples of brain function such as the capacity for consciousness or ability for unassisted breathing should be distinguished from examples of brain activity such as posterior pituitary antidiuretic hormone release or residual nests of neuronal electrical activityintegrationcombined or coordinated separate elements that provide a harmonious , interrelated whole ; organized or structured so that constituent units function cooperativelyirreversiblepertaining to a situation or condition that will not or can not return or resume . in the context of death determination , there are variable definitions including :  1.loss of function or a condition that can not be restored by anyone under any circumstances at a time now or in the future  2.loss of function or a condition that can not be restored by those present at the time  3.loss of function or a condition that will not resume and will not be restored . also referred to as permanentneuroimagingdiagnostic brain imaging techniques to identify structural brain injury , e.g. ct scan , mrineurological death determinationdiagnosis and confirmation of death based on neurological criteriano effective interventiona therapeutic intervention that is not deployed because it is not effective , not medically indicated under those circumstances , not available or accessiblemechanical ventilationassisted ventilation including bag / mask ventilation , non - invasive support e.g. bipap ( bilevel positive airway pressure ) , conventional mechanical ventilation via artificial airwayrefractory to treatmentdoes not respond to intervention in a clinically meaningful mannerpermanentpertaining to a situation or condition that will not return to its previous state . in the context of death determination , refers to loss of function that will not resume spontaneously and will not be restored through interventionpreconditionspatient - related prerequisites that should be fulfilled prior to application of diagnostic testsrespiratory arrestcessation of breathing . in the context of death discussions , this may be primary and lead to a subsequent cardiac arrest , or it may be secondary to the loss of brainstem functiontesta procedure performed in diagnosis or detectiontest  ancillarya complementary test or an alternative test to one that otherwise , for any reason , can not be conducted or is unreliabletest clinicala bedside test typically based on physical examination of the patient , but may include the use of a stethoscope and vital signs monitorstest  confirmatorya test performed to confirm a previously conducted testtest laboratorya technical test requiring use of elaborate equipment and medical technologies , e.g. blood testing , diagnostic imagingtest supplementala test performed in addition to an already conducted testunitythe combination or arrangement of parts into a wholeventricular fibrillationa condition in which there is uncoordinated contraction of the cardiac muscle of the ventricles that causes the cessation of circulation . also referred to as v - fib or vfvital functionnecessary for sustained life participants agreed to the following terminology , in order to improve the clarity of discussions and debate , for use during and subsequent to this forum forum participants identified several major sequential events in the dying process of patients who have suffered a catastrophic brain injury that will lead to death determined on a neurological basis ( such as , but not limited to , traumatic brain injury , cerebrovascular accidents , and hypoxic - ischaemic encephalopathy after resuscitated cardiac arrest ) ( fig .  1 ) . this sequence does not apply to patients with anencephaly or with forms of catastrophic brain injury ( such as persistent vegetative states ) where residual clinical brain or brainstem functions are retained ; such patients were not under consideration . patients entering this sequence are receiving mechanical ventilation ; various other neuroprotective interventions ( such as hyperosmolar therapy , ventricular drainage , decompressive craniectomy ) may have been initiated as well . at n-1 , the patient continues to deteriorate in spite of intervention and the treatment team recognizes that the patient may evolve to brain death . at n-2 however , at this point it is still possible that brain function could return spontaneously or be restored through intervention . if preconditions are met , confounding factors are absent , and no effective treatment is available or implemented , then by n-3 , the brain has ceased functioning and there is no possibility to resume . 1neurological sequence in the dying process neurological sequence in the dying process preconditions prior to testing brain function at any point in the sequence include an established etiology ; absence of reversible etiologies that would explain the coma ; and the absence of hemodynamic shock associated with inadequate oxygenated circulation to the brain . confounding conditions that may invalidate testing for cessation of brain function include naturally occurring or therapeutic hypothermia ; the presence of central nervous system ( cns ) depressing drugs that may explain or contribute to coma ; high cervical spine injury ; acquired ( such as severe polyneuropathy ) or therapeutic neuromuscular paralysis ; locked - in syndromes ; and severe acid - base , electrolyte or endocrine abnormalities that may explain or contribute to coma . after agreeing that death is principally established using clinical criteria ( defined as diagnostic testing based on direct , measurable observation or examination of the patient ; see table  1 ) , participants came to consensus on the  minimum acceptable clinical standards  to test for the cessation of brain function ( table  2 ) . it was also agreed that the validity of a determination of death depends upon the health care professionals performing the clinical determination and those performing and interpreting ancillary laboratory testing possessing the necessary competencies.table  2minimum acceptable clinical standard and additional tests for death after cessation of brain function in adults and childrendescriptionminimum acceptable clinical standardadditional testing ( beyond the minimum clinical standard)n-1catastrophic brain injury : continuing deterioration and progressive loss of brain function despite intervention1 . evidence for progressing loss of brainstem function1 . neuroimaging that explains the severity of brain injury2 . absence of brainstem reflexes :  pupils mid - position or greater and absent pupillary light reflex ( fixed dilated pupils )  corneal  gag / pharyngeal  cough / tracheal  vestibulo - ocular (  cold caloric )  loss of central drive to breathe  nb : performance of apnoea testing should be reserved as the last test of brainstem functionnone : cessation of brain function is a clinical determinationn-3cessation of brain function with no possibility to resume1 . ancillary laboratory tests e.g.  demonstration of brain blood flow or perfusion to be absent  refractory intracranial hypertension as measured by icp monitoring  transcranial doppler consistent with absent net flow velocity  electrodiagnostic testing ( e.g. eeg , absent evoked potentials ) minimum acceptable clinical standard and additional tests for death after cessation of brain function in adults and children forum participants identified several major sequential events in the dying process of patients who suffer a circulatory arrest ( fig .  2 ) . in situation a , the patient has had a cardiac arrest but no cpr intervention , either because cpr was not medically indicated or the patient ( or surrogate decision maker ) declined it ; this would include terminally ill patients whose end - of - life care involves limiting or withdrawing life - sustaining therapies . at c-1 , the patient s circulation and breathing stop . after a certain time period ( between 2 and 5  min , based on expert consensus ) , autoresuscitation - the spontaneous , unassisted resumption of circulation - is no longer a possibility under these conditions ( c-2 ) . since no interventions will be made to attempt to restore circulation , cessation of breathing and circulation is permanent and the patient may be determined to be dead ( c-2 and c-3 occur at the same time ) . in situation b , cpr has been used in an attempt to restore circulation and respiration but has been terminated because the patient can not be revived . once the time interval when autoresuscitation is possible has passed , cessation of breathing and circulation is permanent and the patient may be determined to be dead . the most common waiting period is 5  min with a range from 2 to 10  min . the participants came to consensus on the  minimum acceptable clinical standards to test for the cessation of circulatory function ( table  3).fig . 2circulatory sequence in the dying processtable  3minimum acceptable clinical standard and additional tests for death after cessation of circulatory function in adults and childrendescriptionminimum acceptable clinical standardadditional testing ( beyond the minimum clinical standard)c-1cessation of circulation and breathing1 . loss of pulsatile arterial blood pressure by non - invasive measurement6 . coma and fixed dilated pupils7 . absence of pulse by dopplernb : oxygen saturation pulse oximetry is an unreliable indicator of absence of pulsatile circulationc-2cessation of circulation and breathing with no possibility to resume spontaneously1 . the persistence of c-1 criteria over a period of time as confirmed by continuous observation and intermittent confirmation including repetition of this evaluation at the end of the period . the time period required is 25  min2 . when breathing and circulation cease following terminated cpr , the time period to reach the point of  no possibility to resume spontaneously use of the same tests for a higher clinical / laboratory standard for c-1 applied after the time interval required to progress from c-1 to c-2 ( 210 min following termination of cpr)c-3cessation of circulation and breathing with no possibility to resume1 . when cpr will not be provided ( patient fulfils criteria for not providing cpr ) c-3 occurs at the moment of c-22 . following termination of cpr , including a decision not to reinstitute cpr , c-3 and c-2 occur at the same time1 . nothing in addition to those tests required for c-2 circulatory sequence in the dying process minimum acceptable clinical standard and additional tests for death after cessation of circulatory function in adults and children the inextricable link between circulation and brain function means that the neurological and circulatory sequences integrate at several points in the dying process of patients who have suffered a circulatory arrest ( fig .  3 ) . once circulation and breathing cease ( c-1 ) , there is a short time period between c1 and n2 ( < 20  s ) during which brain function ceases ( n-2 ) as evidenced by isoelectric eeg [ 1921 ] . the longer the time period without oxygenated circulation to the brain ( n-2 to n-3 ) the progressively higher likelihood that the cessation of brain function is irreversible , even if oxygenated circulation can be re - established ( either spontaneously or through intervention ) . the precise time period for the complete cessation of brain function to be non - resuscitable ( through intervention ) is unresolved.fig . 3physiological sequences in the dying process : integrated neurological and circulatory sequence ( applies to patients suffering a circulatory arrest ) physiological sequences in the dying process : integrated neurological and circulatory sequence ( applies to patients suffering a circulatory arrest ) after reviewing the historical taxonomy and definitions of death , and the neurologic and circulatory sequences in the dying process that had been previously discussed and refined , participants came to consensus on an operational definition of human death , that is , a practical and quantifiable description of the state of human death based on measurable and observable biomedical standards . forum participants agreed on the following operational definition of death : death is the permanent loss of capacity for consciousness and all brainstem functions . this may result from permanent cessation of circulation or catastrophic brain injury . in the context of death determination , permanent refers to loss of function that can not resume spontaneously and will not be restored through intervention . participants supported avoiding use of anatomically based terms such as  brain death  or  cardiac death  that erroneously imply the death of that organ and confuse the general public , health professionals , and policymakers ( organisms die , while organs cease functioning ) . our operational definition is based on the cessation of function ( the primary and fundamental purpose of an organ that can be assessed by observation and examination and is necessary for sustained life ) rather than activities ( physiologic properties of cells and groups of cells that can be measured by laboratory means ) . while the forum participants understood that the overwhelming majority of deaths in the world occur after circulation has ceased , and many occur outside health care settings , death determination must focus on the centrality of brain function . death is a single phenomenon based on permanent cessation of brain function ( loss of capacity for consciousness and brainstem reflexes ) , which occurs along two pathways : ( 1 ) permanent absence of circulation or ( 2 ) subsequent to a catastrophic brain injury , each discerned through a specific set of medical criteria and clinical and laboratory tests  two entrances , one end point . forum participants identified several major sequential events in the dying process of patients who have suffered a catastrophic brain injury that will lead to death determined on a neurological basis ( such as , but not limited to , traumatic brain injury , cerebrovascular accidents , and hypoxic - ischaemic encephalopathy after resuscitated cardiac arrest ) ( fig .  1 ) . this sequence does not apply to patients with anencephaly or with forms of catastrophic brain injury ( such as persistent vegetative states ) where residual clinical brain or brainstem functions are retained ; such patients were not under consideration . patients entering this sequence are receiving mechanical ventilation ; various other neuroprotective interventions ( such as hyperosmolar therapy , ventricular drainage , decompressive craniectomy ) may have been initiated as well . at n-1 , the patient continues to deteriorate in spite of intervention and the treatment team recognizes that the patient may evolve to brain death . at n-2 however , at this point it is still possible that brain function could return spontaneously or be restored through intervention . if preconditions are met , confounding factors are absent , and no effective treatment is available or implemented , then by n-3 , the brain has ceased functioning and there is no possibility to resume . 1neurological sequence in the dying process neurological sequence in the dying process preconditions prior to testing brain function at any point in the sequence include an established etiology ; absence of reversible etiologies that would explain the coma ; and the absence of hemodynamic shock associated with inadequate oxygenated circulation to the brain . confounding conditions that may invalidate testing for cessation of brain function include naturally occurring or therapeutic hypothermia ; the presence of central nervous system ( cns ) depressing drugs that may explain or contribute to coma ; high cervical spine injury ; acquired ( such as severe polyneuropathy ) or therapeutic neuromuscular paralysis ; locked - in syndromes ; and severe acid - base , electrolyte or endocrine abnormalities that may explain or contribute to coma . after agreeing that death is principally established using clinical criteria ( defined as diagnostic testing based on direct , measurable observation or examination of the patient ; see table  1 ) , participants came to consensus on the  minimum acceptable clinical standards  to test for the cessation of brain function ( table  2 ) . it was also agreed that the validity of a determination of death depends upon the health care professionals performing the clinical determination and those performing and interpreting ancillary laboratory testing possessing the necessary competencies.table  2minimum acceptable clinical standard and additional tests for death after cessation of brain function in adults and childrendescriptionminimum acceptable clinical standardadditional testing ( beyond the minimum clinical standard)n-1catastrophic brain injury : continuing deterioration and progressive loss of brain function despite intervention1 . evidence for progressing loss of brainstem function1 . neuroimaging that explains the severity of brain injury2 . absence of brainstem reflexes :  pupils mid - position or greater and absent pupillary light reflex ( fixed dilated pupils )  corneal  gag / pharyngeal  cough / tracheal  vestibulo - ocular (  cold caloric )  loss of central drive to breathe  nb : performance of apnoea testing should be reserved as the last test of brainstem functionnone : cessation of brain function is a clinical determinationn-3cessation of brain function with no possibility to resume1 . ancillary laboratory tests e.g.  demonstration of brain blood flow or perfusion to be absent  refractory intracranial hypertension as measured by icp monitoring  transcranial doppler consistent with absent net flow velocity  electrodiagnostic testing ( e.g. eeg , absent evoked potentials ) minimum acceptable clinical standard and additional tests for death after cessation of brain function in adults and children forum participants identified several major sequential events in the dying process of patients who suffer a circulatory arrest ( fig .  2 ) . in situation a , the patient has had a cardiac arrest but no cpr intervention , either because cpr was not medically indicated or the patient ( or surrogate decision maker ) declined it ; this would include terminally ill patients whose end - of - life care involves limiting or withdrawing life - sustaining therapies . at c-1 , the patient s circulation and breathing stop . after a certain time period ( between 2 and 5  min , based on expert consensus ) , autoresuscitation - the spontaneous , unassisted resumption of circulation - is no longer a possibility under these conditions ( c-2 ) . since no interventions will be made to attempt to restore circulation , cessation of breathing and circulation is permanent and the patient may be determined to be dead ( c-2 and c-3 occur at the same time ) . in situation b , cpr has been used in an attempt to restore circulation and respiration but has been terminated because the patient can not be revived . once the time interval when autoresuscitation is possible has passed , cessation of breathing and circulation is permanent and the patient may be determined to be dead . the most common waiting period is 5  min with a range from 2 to 10  min . the participants came to consensus on the  minimum acceptable clinical standards to test for the cessation of circulatory function ( table  3).fig . 2circulatory sequence in the dying processtable  3minimum acceptable clinical standard and additional tests for death after cessation of circulatory function in adults and childrendescriptionminimum acceptable clinical standardadditional testing ( beyond the minimum clinical standard)c-1cessation of circulation and breathing1 . absence of pulse by dopplernb : oxygen saturation pulse oximetry is an unreliable indicator of absence of pulsatile circulationc-2cessation of circulation and breathing with no possibility to resume spontaneously1 . the persistence of c-1 criteria over a period of time as confirmed by continuous observation and intermittent confirmation including repetition of this evaluation at the end of the period . the time period required is 25  min2 . when breathing and circulation cease following terminated cpr , the time period to reach the point of  no possibility to resume spontaneously use of the same tests for a higher clinical / laboratory standard for c-1 applied after the time interval required to progress from c-1 to c-2 ( 210 min following termination of cpr)c-3cessation of circulation and breathing with no possibility to resume1 . when cpr will not be provided ( patient fulfils criteria for not providing cpr ) c-3 occurs at the moment of c-22 . following termination of cpr , including a decision not to reinstitute cpr , c-3 and c-2 occur at the same time1 . nothing in addition to those tests required for c-2 circulatory sequence in the dying process minimum acceptable clinical standard and additional tests for death after cessation of circulatory function in adults and children the inextricable link between circulation and brain function means that the neurological and circulatory sequences integrate at several points in the dying process of patients who have suffered a circulatory arrest ( fig . once circulation and breathing cease ( c-1 ) , there is a short time period between c1 and n2 ( < 20  s ) during which brain function ceases ( n-2 ) as evidenced by isoelectric eeg [ 1921 ] . the longer the time period without oxygenated circulation to the brain ( n-2 to n-3 ) the progressively higher likelihood that the cessation of brain function is irreversible , even if oxygenated circulation can be re - established ( either spontaneously or through intervention ) . the precise time period for the complete cessation of brain function to be non - resuscitable ( through intervention ) is unresolved.fig . 3physiological sequences in the dying process : integrated neurological and circulatory sequence ( applies to patients suffering a circulatory arrest ) physiological sequences in the dying process : integrated neurological and circulatory sequence ( applies to patients suffering a circulatory arrest ) after reviewing the historical taxonomy and definitions of death , and the neurologic and circulatory sequences in the dying process that had been previously discussed and refined , participants came to consensus on an operational definition of human death , that is , a practical and quantifiable description of the state of human death based on measurable and observable biomedical standards . forum participants agreed on the following operational definition of death : death is the permanent loss of capacity for consciousness and all brainstem functions . this may result from permanent cessation of circulation or catastrophic brain injury . in the context of death determination , permanent refers to loss of function that can not resume spontaneously and will not be restored through intervention . participants supported avoiding use of anatomically based terms such as  brain death  or  cardiac death  that erroneously imply the death of that organ and confuse the general public , health professionals , and policymakers ( organisms die , while organs cease functioning ) . our operational definition is based on the cessation of function ( the primary and fundamental purpose of an organ that can be assessed by observation and examination and is necessary for sustained life ) rather than activities ( physiologic properties of cells and groups of cells that can be measured by laboratory means ) . while the forum participants understood that the overwhelming majority of deaths in the world occur after circulation has ceased , and many occur outside health care settings , death determination must focus on the centrality of brain function . death is a single phenomenon based on permanent cessation of brain function ( loss of capacity for consciousness and brainstem reflexes ) , which occurs along two pathways : ( 1 ) permanent absence of circulation or ( 2 ) subsequent to a catastrophic brain injury , each discerned through a specific set of medical criteria and clinical and laboratory tests  two entrances , one end point . this report describes the initial phase in an international process to develop guidelines and agree upon an operational definition for determining death , based on the cessation of neurological and circulatory functions . plenary discussions resulted in consensus on seven key areas : death is determined primarily using clinical criteria based on direct observation or examination of the patient , once preconditions have been fulfilled and confounding conditions excluded.the physiological sequences by which circulatory and neurological functions cease , leading up to the moment of death , were set forth to clarify the critical events that occur following a catastrophic injury or illness.clinical tests that constitute the minimum clinical standard for the determination of death were defined for both the neurological and the circulatory sequences . preconditions and confounding conditions that may impede or invalidate death diagnosis were also specified.certain ancillary and/or complementary laboratory tests may be useful in situations in which clinical testing can not be performed or when confounding or special conditions are present ; limitations to using certain of these tests in death determination mean that further research is required to ensure their reliability.a set of precise terminology , which aims to improve clarity in death determination discussions and debate , was agreed upon.an operational definition of human death , based on measurable biomedical standards , was proposed . participants supported avoiding use of anatomically based terms such as  brain death  or  cardiac death  that erroneously imply the death of an organ . emphasis was placed on the cessation of neurological or circulatory function , and the centrality of brain function for determination of death . death occurs when there is permanent loss of capacity for consciousness and loss of all brainstem functions . in the context of death determination ,  permanent refers to loss of function that can not resume spontaneously and will not be restored through intervention . this definition is based on the cessation of function ( the primary and fundamental purpose of an organ that can be assessed by observation and examination and is necessary for sustained life ) rather than activities ( physiologic properties of cells and/or groups of cells that can be measured by laboratory means).in order to complete the elaboration of an operational definition of human death that could be used in countries around the world , a broader group of international stakeholders will be needed to develop clinical practice guidelines , based on comprehensive , systematic reviews and grading of existing evidence . death is determined primarily using clinical criteria based on direct observation or examination of the patient , once preconditions have been fulfilled and confounding conditions excluded . the physiological sequences by which circulatory and neurological functions cease , leading up to the moment of death , were set forth to clarify the critical events that occur following a catastrophic injury or illness . clinical tests that constitute the minimum clinical standard for the determination of death were defined for both the neurological and the circulatory sequences . certain ancillary and/or complementary laboratory tests may be useful in situations in which clinical testing can not be performed or when confounding or special conditions are present ; limitations to using certain of these tests in death determination mean that further research is required to ensure their reliability . a set of precise terminology , which aims to improve clarity in death determination discussions and debate , was agreed upon . an operational definition of human death , based on measurable biomedical standards , was proposed . participants supported avoiding use of anatomically based terms such as  brain death  or  cardiac death  that erroneously imply the death of an organ . emphasis was placed on the cessation of neurological or circulatory function , and the centrality of brain function for determination of death . death occurs when there is permanent loss of capacity for consciousness and loss of all brainstem functions . this may result from permanent cessation of circulation or catastrophic brain injury . in the context of death determination , permanent refers to loss of function that can not resume spontaneously and will not be restored through intervention . this definition is based on the cessation of function ( the primary and fundamental purpose of an organ that can be assessed by observation and examination and is necessary for sustained life ) rather than activities ( physiologic properties of cells and/or groups of cells that can be measured by laboratory means ) . in order to complete the elaboration of an operational definition of human death that could be used in countries around the world , a broader group of international stakeholders will be needed to develop clinical practice guidelines , based on comprehensive , systematic reviews and grading of existing evidence . participating organizations : canadian blood services ; australia and new zealand intensive care society ; canadian critical care society ; european society of intensive care medicine ; international federation of emergency medicine ; international pan arab critical care medicine society ; international society for organ donation and procurement ; neurocritical care society ; society of critical care medicine ; united kingdom faculty of intensive care medicine ; university of ottawa loeb chair and research consortium in organ and tissue donation ; world federation of neurology ; world federation of neurosurgical societies ; world federation of pediatric intensive and critical care societies ; world federation of societies of intensive and critical care medicine ; world health organization . sadek beloucif , france ; dr . mohammed salah ben ammar , tunisia ; dr . james l. bernat , department of neurology , geisel school of medicine at dartmouth ; dr . alexander m. capron , professor of law and medicine , university of southern california ; dr . geoff dobb , australia and new zealand intensive care society ; laura hornby , loeb chair and research consortium in organ and tissue donation ; dr . edgar jimenez , world federation of societies of intensive and critical care medicine ; dr . gnter kirste , international society for organ donation and procurement ; dr . niranjan kissoon , world federation of pediatric intensive and critical care societies ; dr . tong kiat kwek , singapore ; dr . alexander manara , uk faculty of intensive care medicine ; dr . luck noel , world health orgnization ; dr .