an apparatus for healing wounds as described herein includes a multi - layer dressing including a hydrophilic hydrogel substance containing glycerin and at least one conduit for removing fluid from the wound ( e . g ., a drain ). an apparatus as described herein can be applied to a wound for at least three ( e . g ., 3 , 4 , 5 , 6 , 7 , 8 ) contiguous days . in brief overview , referring to fig1 - 3 , a first embodiment of an apparatus for healing wounds 10 as described herein is shown applied to a wound bed 15 and includes several layers . a first layer 20 of hydrophilic hydrogel substance is positioned on top of and covering the wound bed 15 . first layer 20 is a thin layer of hydrophilic hydrogel substance of any suitable thickness ( e . g ., 1 / 20 , 1 / 19 , 1 / 18 , 1 / 17 , 1 / 16 , 1 / 10 , ⅛ , ⅙ , ¼ of an inch ), and is typically about 1 / 16 of an inch . first layer 20 protects the wound bed 15 from direct contact with the first conduit 30 ( described below ) and provides a moist environment for healing . the hydrophilic hydrogel substance is a blend of glycerin with synthetic hydrophobic polymers in a two - component system in which water enhances the compatibility and function of the dressing and is described in u . s . pat . no . 5 , 961 , 479 . in a typical apparatus , each layer of hydrophilic hydrogel substance has a glycerin content in the range of about 65 - 70 % ( e . g ., 50 %, 55 %, 60 %, 65 %, 70 %, 75 %, etc .) that contributes to the dressing &# 39 ; s positive anti - microbial properties , water soluble humectant in a mixture of water ( in the range of about 15 - 20 %) and a polyacrylamide ( in the range of about 15 - 20 %). in an example of one embodiment , the hydrophilic hydrogel substance has a glycerin content of approximately 65 %, a water content of about 17 . 5 %, and a polyacrylamide content of about 17 . 5 %. the ph of a hydrophilic hydrogel substance is generally between 5 and 6 . use of the hydrogel provides for the absorbtion of high quantities of fluid ( exudate ) from the wound . due to the high percentage of glycerin , the use of hydrophilic hydrogel substances in the apparatuses as described herein confers additional desirable properties , such as bacteriostatic / fungistatic action , retention of growth factors at the wound site , and self - debriding activity , which are absent in conventional hydrogel dressings of high water content . on top of the first layer 20 is placed a first conduit 30 for removing fluid from the wound . the first conduit 30 is operably connected to a vacuum pump which is operably connected to vacuum pump controller 120 , and is typically a drain . the first conduit 30 can be made of any suitable material , e . g ., medical - grade plastic tubing , and the dimensions of the first conduit 30 may vary depending on the dimensions of the wound . the vacuum pump and the vacuum pump controller 120 supply suction to the wound bed 15 and at the peripheries of the wound ( i . e ., wound bed edge 16 ) to remove the dissolved hydrogel substance as the hydrogel substance removes wound exudates from the wound bed 15 and migrates to the dressing periphery . as the first conduit 30 removes fluids exuding from the wound bed 15 ( exudate ), it transfers the fluids to a sump collecting chamber 110 or other suitable collection device via tube connector 80 . in some embodiments , depending on the type of wound , an apparatus 10 as described herein can further include a second conduit 50 for removing fluids from the wound . when treating wounds such as large bums , traumatic wounds , and diabetic pressure ulcers , for example , an apparatus having a second conduit 50 may be preferable . the second conduit 50 ( also referred to as “ peripheral drainage tube ” or “ peripheral drain ”) is positioned at the edges of the wound bed 16 or exterior to the wound , extending along the length or circumference of the wound . the second conduit 50 can be made of any suitable material , e . g ., medical - grade plastic tubing , and the dimensions of the second conduit 50 may vary depending on the dimensions of the wound . the second conduit 50 is typically perforated plastic tubing having openings along its length for taking up fluids and is also attached to a vacuum pump and sump collecting chamber 110 as is the first conduit 30 . as the first layer 20 of hydrogel dressing material absorbs greater amounts of wound exudates , the captured fluids are released in a unique combination of wound exudate and dissolved hydrogel - dressing material , which is collected by the first conduit 30 and at the edges of the wound bed 16 by the second conduit 50 . in a typical apparatus , a second layer 40 of gauze impregnated with hydrophilic hydrogel substance is positioned above the first conduit 30 . this second layer 40 packs the wound and provides a moist environment for healing . the gauze can be any suitable gauze for wound dressings , and is combined with any suitable amount of high - glycerin content hydrophilic hydrogel substance ( as described in u . s . patent application no . 5 , 961 , 479 ). gauze provides deposition of a fine film on the wound bed for water molecule transmission to the larger layer of hydrogel above it ( i . e ., third layer 60 described below ). although gauze is typically used in the apparatuses and dressings described herein , any material that provides a scram or gauze - like texture made of a rectangular weave , coarse cotton or synthetic fiber that will provide a rectangular weave to which the hydrogel material can cling , providing a web - like matrix that can be spread across the wound bed to cover the wound completely , can be used . the next layer of the apparatus 10 is a third layer 60 of hydrophilic hydrogel substance that extends beyond the wound bed 15 and adheres to the skin 70 surrounding the wound , creating a seal between the wound bed 15 and ambient air . the hydrophilic hydrogel substance of third layer 60 , of second layer of 40 , and of first layer 20 are typically the same material ( as described in u . s . pat . no . 5 , 961 , 479 ), varying generally only in thickness . different hydrophilic hydrogel substances , however , can be used in the first , second , and third layers of an apparatus if appropriate . like the other layers containing hydrophilic hydrogel substance , third layer 60 is typically a clear , semi - permeable hydrogel dressing , which can be sized to extend a suitable distance ( e . g ., 0 . 5 , 1 . 0 , 1 . 5 , 2 . 0 , 2 . 5 inches ) beyond the dimensions of the wound . third layer 60 protects the adjacent skin , provides an occlusive bond with the skin 70 surrounding the wound , and provides a moist environment for healing . the first conduit 30 is mostly below the third layer 60 , but a portion of the first conduit 30 penetrates the third layer 60 and operably connects to tube connector 80 and sump collection chamber 110 . to maintain a seal between the wound bed 15 and ambient air , the portion of third layer 60 that surrounds the point of penetration can be pinched together and sealed , or ostomy paste can be placed around the point of penetration to maintain the seal . in some embodiments ( e . g ., fig1 and 2 ), an apparatus for healing wounds as described herein further includes a photon - emitting device 90 that is operably connected to a control device . in such embodiments , the photon - emitting device 90 typically provides near infrared light therapy ( nilt ) to the wound . as wound healing moves through the stages of inflammation , proliferation , remodeling and maturation , nilt can have a positive impact on each of these phases . nilt irradiation can provide a beneficial impact on both closed connective and soft tissue injuries as well as in open wounds in a number of ways . nilt enhances leukocyte ( white cell ) infiltration to protect the injured tissues . nilt accelerates macrophage activity in phagocytosis ( destroying bacteria and foreign material ), growth factor secretion ( to stimulate tissue repair ) and stimulation of collagen synthesis ( for tissue integrity ), all of these factors contributing to acceleration of healing . significant angiogenesis ( new vessel growth ) occurs with nilt promoting new capillary growth resulting in subsequent improvement in blood perfusion and oxygenation . endothelial cell regeneration is also accelerated . nilt irradiation stimulates an increase in fibroblast numbers and fibroblast - mediated collagen production . the beneficial synthesis activities and growth factor ability of keratinocytes are enhanced by proliferation secondary to nilt irradiation . nilt - stimulated acceleration of epithelial cell regeneration speeds up wound healing , minimizes scarring and reduces the opportunity for infection . a two - to five - fold increase in growth - phase - specific dna synthesis in normal fibroblasts , muscle cells , osteoblasts and mucosal epithelial cells irradiated with near infrared light has been reported . this has increased levels of vascular endothelial growth factor ( vegf ) and fibroblast growth factor ( fgf - 2 ) secondary to nilt . nilt - induced increases in no , atp and other compounds that stimulate higher activity in cell proliferation and differentiation cause an increase in mature cells . the increased numbers of myofibroblasts , myofibrils , and myotubes as well as bone cell proliferation have been clinically documented after ntlt irradiation . satellite cells , the precursor cells to muscle regeneration , show significant increase in proliferation when irradiated with nilt . studies have shown that nilt results in greater healed wound tensile strength in both soft tissue and connective tissue injuries . nilt can increase the final tensile strength of the healed tissue by increasing the amount of collagen production / synthesis and by increasing the intra - and inter - molecular hydrogen bonding in the collagen molecules . studies have also shown that nilt has a significant effect on damaged cells and tissues while normal biological constituents of healthy tissues are appreciably less affected . methods for using nilt to heal wounds are described , for example , in rochkind et al ., lasers in surgery and medicine 9 : 174 - 182 ( 1989 ); braverman et al ., lasers in surgery and medicine 9 : 50 - 58 ( 1989 ); and surinchak et al ., lasers in surgery and medicine 2 : 267 - 274 ( 1983 ). when in use , the photon - emitting device 90 is placed directly over the third layer 60 and is connected to a control device via wires 100 or leads . any suitable photon - emitting device can be used . in a typical embodiment , the photon - emitting device is a led that provides near infrared stimulation to the wound . use of a photon - emitting device and nilt in apparatuses and methods described herein is described in greater detail below . referring now to fig4 , two embodiments of an apparatus for healing wounds 10 are shown . like the embodiments shown in fig1 - 3 , the apparatuses of fig4 include a first conduit 30 and a second conduit 50 ( e . g ., peripheral drain ). in fig4 a , the apparatus 10 includes a first conduit 30 that is referred to as a “ flat drain .” this type of drain is placed on top of the first layer 20 . in fig4 b , the apparatus 10 includes what is referred to as a “ channel drain ” which is particularly useful for healing wounds in which an object has penetrated or stabbed the tissue , leaving a deep puncture wound in the wound bed . this type of drain is also placed on top of the first layer 20 , but this drain penetrates the first layer 20 and descends into the puncture . there are several advantages to using an apparatus for healing wounds including a hydrophilic hydrogel substance containing glycerin and at least one conduit for removing fluid from the wound . due to the high permeability , water content , and ph of the hydrophilic hydrogel dressings used in apparatuses of the invention , pain can be reduced by protecting neurons from dehydration . the cooling effect on wound surfaces may result from the dressing &# 39 ; s modulating effect in reducing inflammatory reactions . other major characteristics of the dressings described herein are elasticity , strength , and durability , which allow the dressing to remain in place for several ( e . g ., seven ) days . as a chemical structure , hydrogels are a three - dimensional network of hydrophilic polymers that interact with aqueous solutions by swelling to certain equilibriums dictated by their different compositions , thus , retaining a significant proportion of water within their structure . hydrogels are insoluble in water and are non - degradable . there are additional advantages to using apparatuses and methods described herein that further include a photon - emitting device such as an led that provides nilt . the photon - emitting device ( e . g ., led ) provides a visible red or near infrared energy to a wound to promote wound healing ( e . g ., lymphatic drainage , cellular growth , angiogenesis and tissue migration facilitating closure of the wound ). light therapy has been shown to deliver powerful therapeutic benefits to living tissues . both visible red and infrared light have shown that they can cause at least 24 different positive changes at the cellular level of tissue . visible red light , at a wavelength of 630 nanometers ( nm ), penetrates tissue to a depth of about 8 - 10 millimeters ( mm ) and is very beneficial in treating problems close to the surface of the skin . infrared light ( 904 nm ) can penetrate to a depth of about 30 - 40 mm , which makes it more effective for treating bones , joints , deep muscle . fig5 shows the results of treatment of a wound with nilt . a 660 nm ( red ) source of 100 joules per cm 2 was applied to a wound over a ten minute treatment period . an optical probe was used that consisted of an array of 48 monochromatic sources operating at a wavelength of 660 nm and covering an area 6 × 10 cm 2 . the photographs of fig5 were taken before treatment , 3 weeks after near infrared irradiation , and 6 weeks after near infrared irradiation treatments . in this experiment , 13 week - old pressure sores were treated twice a week for 10 minutes with an led cluster probe . two optical probes were used , one consisted of an array of 22 monochromatic sources , operating at a wavelength of 660 nm and covering an area 6 × 10 cm 2 . the second probe had seven infrared sources , operating at a wavelength of 880 nm and covering an area of 4 cm 2 . histological inspection showed that infiltration of fibroblasts in the subcutis was significantly greater in the far - infrared ( fir ) irradiation group than in the group without fir irradiation on days 1 , 5 , and 7 . furthermore , there was significantly greater collagen regeneration in the fir group than in the group without fir on day 7 in sections with mallory &# 39 ; s staining . the number of migrated fibroblasts expressing tgf - β1 was significantly greater in the fir irradiation group , and the production of collagen was increased in the fir group . the production of collagen fibers due to the activation of fibroblasts by fir irradiation has been considered as a possible mechanism for the promotive effect of fir irradiation on wound healing . the combination of a hydrogel dressing , negative pressure vacuum removal of exudate , and photonic emission ( e . g ., nilt ) provides an ideal environment within which non - healing wounds ( e . g ., chronic wounds ) can begin to re - epithelialize . use of such an apparatus on a wound promotes angiogenesis , cellular growth , and lymphatic drainage due to the near infrared radiation produced by an array of leds and / or laser diodes in several wavelengths coupled with wound healing effects of a high glycerin content containing dressing . although fig1 and 2 illustrate a photon - emitting device ( e . g ., led display ) placed on top of the dressing , radiating through the dressing material to the wound below , in other embodiments , diodes can be incorporated in the hydrogel dressing material . the diodes can be activated and controlled by either a direct electrical connection or by induction from the timer controller , which is either an independent stand - alone control or a module in a negative pressure vacuum pump device . the led timer typically has multiple settings to control near infrared radiation treatment periods . the combined effects of the near infrared stimulation and the hydrogel dressing can improve wound - healing dynamics of chronic wounds such as pressure sores , diabetic and stasis ulcers , among others , as well as new wounds ( e . g ., surgical wounds , accidental wounds ) and skin flaps . several studies support the fact that the dressings described herein containing hydrophilic hydrogel substances ( gels as described in u . s . pat . no . 5 , 961 , 479 ) do not support growth of any microbe tested but instead kill bacteria that are able to survive on inert surfaces ( in such studies , only bacillus subtilus , a gram - positive rod that can form spores , was not killed ). glycerin in high concentrations has a slight but definite anti - microbial action , which accounts for the way bacterial growth is hampered . it is expected that bacterial size precludes penetration of the gel by bacteria in hydrophilic hydrogel dressings as described herein . glycerin also appears to have an immunomodulating effect , which influences the inflammatory response to injury . cell cultures of human lymphocytes are hampered in their reaction to foreign epidermal cells in the presence of glycerin in even the smallest amounts . glycerin &# 39 ; s strong negative charge binds to extra cellular matrix molecules , modifying their break down and subsequently modulating the inflammatory response . the bilaminate construction of the hydrophilic hydrogel dressings used in apparatuses and methods as described herein provides a homogeneous hydrogel composite dressing deposited on a mechanically stable substrate , such as a knit like material , which fits comfortably over the wound and creates intimate contact with all surfaces , while protecting and promoting early healing of wound trauma created by laser , chemical deep peelings , derma - abrasion , or delicate plastic / re - constructive or surgical incisions or ulcers . bilaminate construction helps reduce the normally high - water - vapor transmission rates often associated with hydrogel dressings to much lower , clinically acceptable levels . by maintaining a moist environment the wound dressing decreases the chances of contamination and bacterial infection and initiates immediate pain relief . mechanically , the layered construction of the apparatuses described herein protects the underlying hydrogel from tearing and puncturing while ensuring conformability to the wound site . apparatuses and methods for healing wounds as described herein including use of a photon - emitting device that provides nilt can be used to reduce acute inflammation at a wound site . nilt can be effective in mediating the underlying inflammatory process by several actions including : restoring polarity and stability of cellular membrane element concentrations of calcium , sodium and potassium ions as well as the proton gradient over the mitochondria membrane , increasing atp production and synthesis which contributes to cellular repair , reproduction and function , inducing vasodilation which reduces ischemia ( lack of oxygen ) and improved perfusion ( blood circulation ), acceleration of leukocytic ( white cell ) activity resulting in enhanced removal of non - viable cellular and tissue components and allowing a more rapid repair and revitalization process , reducing the effect of pro - inflammatory cytokines that have been implicated in the development of inflammatory conditions , enhancing the lymphocyte response , and increasing cytokine superoxide dismutase ( sod ), a powerful antioxidant , levels . since nilt does not exacerbate the inflammatory process but rather condenses the time frame from onset to resolution , it can be used immediately after an injury . this rapid initiation of therapy after acute inflammation occurs can assist in limiting the scope and duration of the inflammatory event and minimize the pain and severity associated with it . as nilt is initiated in more chronic inflammatory conditions , the treatment regimen and course of therapy may be modified by addition of the time required for the desired response , but , the physiological responses and interactions remain consistent . apparatuses and methods for healing wounds as described herein can also be used to reduce pain caused by a wound . the unique pain - reducing abilities of nilt irradiation have been researched and documented in numerous clinical studies and medical papers . nilt provides pain relief by several mechanisms , including : increasing b - endorphin production , blocking depolarization of c - fiber afferent nerves , increasing nitric oxide production , increasing nerve cell action potential , nerve cell regeneration and axonal sprouting , decreasing bradykinin levels , and increasing release of acetylcholine . the invention also includes kits for healing wounds . a typical kit includes at least two sheets of hydrophilic hydrogel substance , a suitable amount of gauze impregnated with hydrophilic hydrogel substance , and instructions for use . in some embodiments , a kit can also include one or more conduits for removing fluids . a suitable amount of gauze impregnated with hydrophilic hydrogel substance is an amount sufficient to cover the wound bed . a sheet of hydrophilic hydrogel substance is a layer of hydrophilic hydrogel substance adhered to a non - adherent backing ( e . g ., a plastic film ). in a typical method of using a kit as described herein , the wound is first irrigated thoroughly with 30 ml of saline solution and is patted dry . a first layer of hydrophilic hydrogel substance is removed from its backing and is placed on the wound bed , covering all of the wound . next , a conduit for removing fluids ( e . g ., a drain ) sized to fit the wound is placed on top of the first layer . care is taken so that the conduit for removing fluids is not placed such that it is directly contacting the wound or placed into any unexplored fistula tract . after the conduit for removing fluids is positioned , gauze impregnated with hydrophilic hydrogel substance is packed on top of the conduit and the first layer , completely covering the conduit . a third layer of hydrophilic hydrogel substance is then placed over the filled wound , leaving a suitable border ( e . g ., two inch border ) that extends beyond the edges of the wound . because the conduit penetrates the third layer so that it can be connected to a sump collection chamber and vacuum pump controller , the third layer around the site of penetration is pinched or crimped to create a seal , or a suitable amount of ostomy paste can be placed around the site of penetration to create a seal . an airtight closure is required when negative pressure is applied by the vacuum pump . the conduit can be secured to the sump collection chamber by using adhesive tape . after an airtight seal is created , the vacuum pump controller settings are typically set between 60 - 80 mm hg and the vacuum pump is turned on . if the dressing fails to contract when negative pressure is applied , it is not sufficiently sealed . to sufficiently seal the dressing , the area of penetration around the conduit can be reinforced , and / or the connection between the conduit and the sump collection chamber can be reinforced . the present invention is further illustrated by the following specific examples . the examples are provided for illustration only and should not be construed as limiting the scope of the invention in any way . in a typical method of healing a wound , a modified chariker - jeter dressing technique as described in chariker et al ., ( contemp surg 34 : 59 - 63 ( 1989 )) is employed . the technique involves a thin film of hybrid hydrogel dressing in the base of the wound , a peripheral drainage tube and a flat jackson - pratt drain in the center of the wound , with a thin transparent hydrogel film on top of the drain and a connecting tube . an apparatus as described herein is applied to the wound for a period of time sufficient to achieve healing . in this example , a wound to be healed is covered with a clear , semi - permeable hybrid hydrogel dressing , which is cut to fit two inches beyond the dimensions of the wound , thus protecting the adjacent skin and providing an occlusive bond with the dry skin surrounding the wound . a hydrogel impregnated gauze sandwich is made around the flat , silicone jackson - pratt drain . the entire drain sandwich is placed on the wound and covered with another piece of the hybrid hydrogel dressing to create a complete seal . the drain is connected to tubing , which is then connected to the vacuum pump . the movable near infrared led is placed directly over the wound . the led head has multiple branches either 77 leds in 11 branches of 7 each or for 35 leds in 7 branches of 5 each , the latter producing 918 mw or 35 × 26 mw , if vishay irdc are used , driving each with 75 ma . the leads are then attached to a controller which provides a multitude of choices of treatment times and near infrared frequencies . in this method , the vacuum pump is set at 80 mm hg of negative pressure in constant mode ( usupov and yepifanov , vestnik khirugii 4 : 42 - 45 ( 1987 ); wackenfors et al ., wound repair regen 12 : 600 - 606 ( 2004 )). when being used at home , the patient can use negative pressure wound therapy ( npwt ) for six to eight hours in every 24 hour period . therapy is administered during night hours when the patient is sleeping . this facilitates normal activities of daily living and is a unique feature of the treatment modality . a memory in the controller will record patient compliance with dates and recorded treatment time . the controller has an automatic timer as a safe guard against treatment time exceeding an acceptable or desirable length . using this treatment plan , the patient undergoes npwt until the healing of the wound plateaus and therapy can be discontinued . when a healthy bed of granulation tissue is identified , the vacuum therapy can be ended . the patient can then apply a composite hybrid hydrogel dressing every four to seven days to promote reepithelialization . complete healing generally occurs within three weeks and the dressings can be removed . in another method of healing a wound , the components of the apparatus are applied individually to the wound , rather than a “ dressing sandwich ” that is applied to the wound . in a first step of this variation of a method , the wound bed is protected by placing a first layer of thin film of hybrid hydrogel substance on the wound bed . next , a drain is cut to the appropriate length to fit the size of the wound , and the drain is placed over the first layer . next , the wound is filled or packed with a second layer of gauze that is impregnated with hybrid hydrogel substance . the wound is then covered with a third layer of hybrid hydrogel substance ( also referred to as hybrid hydrogel occlusive dressing ) leaving a two inch border that extends beyond the wound to ensure a proper occlusive seal . the drain penetrates this third layer and connects to sump collection chamber which is connected to a vacuum pump controller . to create a seal around the area of penetration , the third layer surrounding the penetration site can be pinched to create a seal , or ostomy paste can be applied around the site of penetration to create a seal . if a photon - emitting device is being used , the device ( e . g ., led ) is next placed on top of the third layer . then , the near infrared timer and negative pressure controller are adjusted to the appropriate settings and turned on . the absorptive qualities of a hydrophilic hydrogel substance used in methods and apparatuses as described herein were tested in an independent test comparing it to the performance of six leading wound dressing materials ( hydrocolloid , membrane , hydrogel and an alginate ) over a test period of ninety - six hours . the absorptive properties were measured by evaluating the absorption of a 0 . 09 % saline solution , simulating wound exudate at room temperature . each dressing was examined at various standard time intervals starting after the first half hour and continuing for a period of ninety - six hours . all but one dressing ( an alginate ) continued to absorb saline for the entire test period . within six hours the hydrogel dressing had absorbed eighty - five grams of fluid , out - performing all of the dressings tested . the vigilon ™ wound dressing absorbed less than twenty grams of water for the entire ninety - six hours . clearsite ™, polymem ™, and duoderm ™ absorbed less than thirty grams of fluid in the ninety - six hour period . polyderm ™ appeared to reach peak absorption at 0 . 5 hours . duoderm ™ began to dissolve after twenty - four hours , so no further measurements were possible . restore ™ absorbed less than seventy grams of fluid in ninety - six hours . at one hour , the invention had absorbed significantly more fluid than any other dressing except kaltostat ™ ( alginate ), which was expected . at three hours the hydrogel dressing had absorbed a similar amount of fluid to that of kaltostat ™, but significantly more than all other dressings . at six hours the hydrogel dressing was the only dressing continuing to absorb significant amounts of fluids . none of the test dressings absorbed more than seventy grams of fluid during the ninety - six hour period except the hydrogel dressing which had absorbed 85 . 0 grams at six hours , 111 . 2 grams at twelve hours , 26 grams at twenty - four hours , 56 . 4 grams at thirty - six hours and 173 . 0 grams at ninety - six hours . heat shock protein and cytokeratin expression , and phase s count in epidermal layer at different times after laser treatment and dermal vascular pattern characterization using a porcine model , back skin was submitted to laser wounds and treated with different occlusive dressings ( flexan , aquaphor ®, vigilon ™ or a hydrophilic hydrogel dressing described herein , either cold or regular ( i . e ., room temperature )) or left exposed to air . the results showed that all topical treatments epithelized faster than untreated air - exposed skin . wounds treated with the hydrophilic hydrogel dressing ( regular or cold ) had a thicker epithelium by day 3 post - treatment than air - exposed skin , suggesting an earlier epithelial maturation time . tissue samples : paraffin blocks from skin used in porcine experiments were used . a total of 4 samples from different animals obtained at day 1 and day 3 post - laser treatment were used for each occlusive dressing . as a control , 4 samples of different animals obtained at the same time , but air - exposed , were used . 4 μm thick sections were obtained from each block . sections were deparaffinized , and rehydrated . on them , the following determinations were performed : low and high molecular weight cytokeratin determination : deparaffinized sections were incubated during 10 minutes with an endogenous peroxidase blocking agent , washed in saline and then incubated for 30 minutes at room temperature with monoclonal sera of commercial origin directed against low or high molecular weight cytokeratins ( biogenex ). reaction was revealed with an immunoenzymatic method using a peroxidase - antiperoxidase ( p . a . p .) commercial kit ( vectastain elite universal kit , vector ). heat shock protein ( hsp ) determination : deparaffinized sections were incubated during 10 minutes with a peroxidase blocking agent , and then incubated for 30 minutes at room temperature with poly or monoclonal sera of commercial origin directed against hsp27 ( biogenex ), hsp70 , hsp84 and hsp104 ( affinity bioreagents , inc ). reaction was revealed with an immunoenzymatic method using a p . a . p . commercial kit ( vectastain elite universal kit , vector ). vascular pattern characterization : in order to better characterize the vascular pattern of injured dermis , blood vessels were subdivided in two groups : one of them corresponding to normal dermal plexus and the other , composed of new blood vessels . differences between these two groups were established by morphological examination and by means of immunoenzymatic determinations of clotting factor viii distribution pattern and actin in deep endothelial cells . normal dermal vessel diameters were measured by means of a morphometric program included in an image processor system coupled to the light microscope ( quantimet 500 +, leica co .). measurements were performed only on round - shaped vessels , discarding those that were cut in a tangential way . at least 10 determinations were performed per sample , and results were expressed in micrometers ( mm ) as the mean of these measurements . neo - formation vessels were counted , referring to an area unit obtained by means of a grid added to the monitor screen , using the same image processor device named before . newly formed vessels are immature ones , so they do not express clotting factor viii in their endothelium nor possess deep endothelial cells ( pericytes ). by determining the presence of pericytes by actin pattern and the maturation grade of endothelial cells by clotting factor viii production , one can distinguish between newly formed and already established vessels . ploidy measurements : alternative sections were stained with a modified acid fuchsin method ( feulgen stain ). sections were analyzed in an image processor system ( zeiss ) with a densitometry program . at least 40 basal cells from each sample were analyzed . the epidermis immediately neighboring the wound was selected as the area to be analyzed . the program selects and numbers cells with a euploid dna content and express the value as a deviation index ( 2cdi ) of dna content of a diploid cell ( diploid cell reference used was lymphocytes or dermal cells ). results were expressed as an histogram , where the value 4 ° c . corresponds to tetraploid cells ( those having a double dna content ), indicating that cells finished the s period of the cell cycle . low molecular weight cytokeratin expression : in day 1 samples , no low molecular weight cytokeratin expression could be demonstrated in either group . by day 3 , a scanty expression could be observed in the regenerating epithelia from samples treated with aquaphor vigilon ™, the hydrophilic hydrogel dressing ( regular or cold ) and from air - exposed skin . flexan - treated skin showed no expression . table 1 summarizes the results obtained . high molecular weight cytokeratin expression : using this serum , no differences could be registered in high molecular weight cytokeratin distribution pattern among different samples in these 2 days checked . hasp expression : in day 1 samples , a weak hsp27 expression was detected on epithelia from all samples . but , by day 3 , regular and cold wounds treated with the hydrophilic hydrogel dressing showed a moderate expression on re - epithelization areas , the expression being less in the wounds treated with regular hydrophilic hydrogel dressing than in the wounds treated with cold hydrophilic hydrogel dressing . the remaining samples showed no changes . other hsps expression showed no differences among samples . table 2 summarizes the results obtained . vascular pattern : by day 1 , all samples showed an increase in vascular diameters of blood vessels placed just below the wound , but , by day 3 , the mean diameters of vigilon ™, air - exposed and hydrophilic hydrogel dressing ( regular and cold )- treated skins increased significantly . among these , cold hydrophilic hydrogel dressing - treated wounds showed the greatest increase . no changes could be registered in the aquaphor and flexan - treated skin . as expected , by day 3 , an angiogenic effect was observed in the wound bed of all samples . neo - formation vessels could be observed in most samples . 1 out of 4 aquaphor treated animals showed an important neo - vascularization process . 3 out of 4 vigilon ™- treated animals showed scanty new vessels . two out of 4 flexan - treated animals showed scanty new vessels , 3 out of 4 regular hydrophilic hydrogel dressing - treated animals showed a moderate blood vessel neo - formation process . the same occurred in 3 out of 4 cold hydrophilic hydrogel dressing - treated animals . on the contrary , only 2 out of 4 air - exposed animals showed scanty new vessels . table 3 summarizes the results obtained when vascular diameters were measured . table 4 summarizes the results obtained when neo - formation vessels were counted . a typical hydrophilic hydrogel substance as described herein is a mixture of water , glycerine , and monomeric acrylamide entrapped in a polymer network of cross - linked polyacrylamide and is prepared by mixing the water , glycerine , monomeric acrylamide , initiator ( e . g ., ammonium persulfate ), and methylene bisacrylamide in appropriate amounts under predetermined reaction conditions which initiate reactions and yield the desired properties . an example of a hydrophilic hydrogel substance is below . ** acrylamide can be purchased as a 50 % solution and stabilized with a maximum of 25 ppm cu + 2 . the maximum concentration in the product is thus about 8 . 3 ppm . the acrylamide solutions contains a maximum of 30 ppm of acrylonitrile , most of which reacts to become part of the polymer during the polymerization . an additional reaction occurs on irradiation sterilization . *** citric acid is added as a stabilizer for the resulting polymer mixture . any improvement may be made in part or all of the components . all references , including publications , patent applications , and patents , cited herein are hereby incorporated by reference . in any listing of possible components , mixtures of possible components are contemplated unless expressly indicated otherwise . the use of any and all examples , or exemplary language ( e . g ., “ such as ”) provided herein , is intended to illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise claimed . any statement herein as to the nature or benefits of the invention or of the preferred embodiments is not intended to be limiting , and the appended claims should not be deemed to be limited by such statements . more generally , no language in the specification should be construed as indicating any non - claimed element as being essential to the practice of the invention . this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law . moreover , any combination of the above - described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contraindicated by context .
the invention relates to the development of apparatuses and methods for healing wounds that combine use of a high glycerin - content hydrogel dressing with negative pressure for removing exudate from a wound . in some embodiments , apparatuses and methods further include a photon - emitting device that delivers near infrared stimulation to the wound for further accelerating wound healing . the apparatuses and methods described herein can be used to heal a variety of wounds , including acute wounds , severe burns , orthopedic and traumatic wounds , skin resurfacing procedure wounds , and neuropathic wounds . the hydrogel dressing is capable of absorbing substantial amounts of fluids from stimulated wet wounds as well as donating substantial amounts of fluids to dry or necrotic wounds , depending upon the moisture content and nature of the substrate to which it is applied . results indicate wounds treated with the apparatuses described herein experience fewer complaints of pain , a decrease in healing time , and a significant cost savings .