referring now in more detail to the figures , fig1 is a perspective view of an embodiment of a detachable transfer conduit 11 . the detachable transfer conduit 11 comprises flexible tubing 13 and a deformable sleeve 15 . the deformable sleeve 15 surrounds a portion of the flexible tubing 13 . the deformable sleeve 15 is attached to the flexible tubing 13 . the deformable sleeve 15 may be attached at any suitable location along the flexible tubing 13 . in a preferred embodiment , the deformable sleeve 15 is attached to the flexible tubing 13 at a location that facilitates substantially sealing , cutting , and detaching the deformable sleeve 15 once cut . in yet another preferred embodiment , the deformable sleeve 15 is adhesively attached to the flexible tubing 13 . attaching the sleeve 15 to the flexible tubing 13 prevents the sleeve 15 from slipping on the tubing away from the point where the flexible tubing 13 is substantially sealed and cut and thus maintains the seal in the flexible tubing 13 . if the sleeve 15 were not attached to the flexible tubing 13 , the sleeve would slip or pull away from the location on the flexible tubing 13 that was sealed , thus breaking the seal and allowing the flexible tubing 13 to open . fig2 depicts an embodiment of a deformable sleeve 15 . the deformable sleeve comprises a wall 17 having an inner surface 19 . in a preferred embodiment , the deformable sleeve is made of a metal . the metal used to make the deformable sleeve may be selected from the group consisting of aluminum , anodized aluminum , brass , bronze , nickel - plated bronze , and stainless steel . however , any suitable material may be used , including metals not listed above . regardless of the material from which the deformable sleeve 15 is constructed , the material has a plasticity such that pressure applied to the flexible tubing 13 at the location of the deformable sleeve 15 causes the sleeve to deform about and substantially seal the flexible tubing 13 before the flexible tubing 13 and deformable sleeve 15 are cut by continued application of pressure . the material also has plasticity such that once cut the deformable sleeve 15 retains its deformed shape and thus substantially seals the flexible tubing 13 . in a preferred embodiment , the deformable sleeve 15 has a length of between about 1 and about 2 inches . however , the deformable sleeve 15 may be of any suitable length . fig3 is an end view of an embodiment of a deformable sleeve 15 . as shown in fig3 , the deformable sleeve 15 has an outer diameter 21 . fig3 also depicts the wall 17 of the deformable sleeve 15 . in a preferred embodiment , the deformable sleeve 15 is substantially cylindrical . however , the sleeve may be shaped in any suitable manner that corresponds with the profile of the flexible tubing 13 , and may , for example , have an oval shaped profile or any other profile . it is understood that the deformable sleeve 15 may be made from a variety of materials or combination of materials , preferably metals , and that depending on the materials used , the wall thickness varies . in one embodiment , the wall 17 has a thickness of between about 0 . 008 and about 0 . 062 inches . in a preferred embodiment , the deformable sleeve 15 is made from aluminum and has a wall thickness of from about 0 . 008 to about 0 . 062 inches . in another preferred embodiment , the deformable sleeve 15 is made from anodized aluminum and has a wall thickness of from about 0 . 008 to about 0 . 062 inches . in yet another preferred embodiment , the deformable sleeve 15 is made from bronze and has a wall thickness of from about 0 . 010 to about 0 . 032 inches . depending on the metal selected , the wall thickness will vary in order to maintain plasticity in the deformable sleeve 15 such that it may be substantially sealed and cut by application of a reasonable amount of pressure . a reasonable amount of pressure would be , for example , that which may be applied by an operator using a manually operated cutting tool . fig4 depicts an embodiment of flexible tubing 13 . as shown in fig4 , the flexible tubing 13 is angled . however , the flexible tubing 13 may be straight or have any other suitable configuration . in one embodiment , the flexible tubing 13 is thermoplastic elastomer tubing such as a styrene isobutylene copolymer ( c - flex ®) or ethylene propylene copolymers blended with polypropylene ( santoprene ®). in another embodiment , the flexible tubing 13 is made from a thermoplastic polymer such as polyvinyl chloride ( pvc ) or a flexible polyolefin such as engage ®. in another embodiment , the flexible tubing 13 is made from a thermoset elastomer such as silicone , fluoro silicone , phenyl silicone , fluoroelastomer ( fkm ), perfluoroelastomer ( ffkm ), or perfluoropolyether ( sifel ®). in yet another embodiment , the flexible tubing 13 is made from a composite material such as a ptfe lined elastomer tube ( smartshield ™ tubing ), perfluoroalkoxy lined thermoplastic tubing ( bev - a - line ®). other material suitable to perform as flexible tubing 13 may be selected and the listing above is not limiting . the deformable sleeve 15 may be attached to the flexible tubing 13 in a variety of ways . in a preferred embodiment , the deformable sleeve 15 is attached to the flexible tubing 13 with a silicone adhesive . in another preferred embodiment , the silicone adhesive is curable platinum catalyzed silicone adhesive . in yet another preferred embodiment , the silicone adhesive is moisture curable silicone adhesive . in one preferred embodiment , the flexible tubing 13 is primed before application of the adhesive and attaching to the deformable sleeve 15 , thereby leaving a layer of primer between the flexible tubing 13 and the deformable sleeve 15 . the primer may also operate to substantially seal the tubing upon the application of pressure and , ultimately , cutting of the flexible tubing 13 and deformable sleeve 15 . in another embodiment of the detachable transfer conduit , the deformable sleeve 15 is attached to the flexible tubing 13 with a hot melt adhesive . this is particularly useful for thermoplastic elastomer tubing . in yet another embodiment of the detachable transfer conduit , the deformable sleeve 15 is attached to the flexible tubing 13 using an adhesive selected from the group consisting of cyanoacrylate , epoxy , and urethane . these adhesives are particularly useful for thermoset elastomer tubing . in another embodiment of the detachable transfer conduit 11 , there is provided a flexible tubing 13 adhesively attached to a deformable sleeve 15 and , where upon application of pressure to the deformable sleeve 13 , the deformable sleeve 15 deforms and substantially seals the flexible tubing 13 and upon application of additional pressure the flexible tubing 13 and deformable sleeve 15 are severed or cut , leaving the flexible tubing 13 sealed where cut . fig5 illustrates a detachable transfer conduit 11 in use with a sampling device 25 and a sample container 22 . the detachable transfer conduit 11 is connected both to the sampling device 25 and the sample container 22 by way of collars 23 . as shown in fig5 , the detachable transfer conduit comprises the flexible tubing 13 and deformable sleeve 15 . in use , an operator may draw a sample from a media receptacle ( not shown ), such as a tank using sample device 25 , thereby filling sample container 22 with a sample . at that point , the operator must disconnect the sample container 22 from the flexible tubing attached to the sampling device 25 . the operator may apply pressure to deformable sleeve 15 , which is adhesively attached to flexible tubing 13 , and deform the sleeve 15 thereby sealing the flexible tubing 13 . the operator may then apply additional pressure to cut the deformable sleeve 15 and the flexible tubing 13 thereby cutting the deformable sleeve 15 and the flexible tubing 13 and leaving the flexible tubing 13 substantially sealed where cut . in a preferred embodiment , the operator quickly applies pressure that substantially seals and then immediately cuts the deformable sleeve 15 . the pressure may be applied with a tool such as a pair of snips or wire cutters or any other tool sufficient to crimp and cut the deformable sleeve 15 and flexible tubing 13 . fig6 provides an embodiment of a detachable transfer conduit 11 that has been cut or severed . in this cut state , there is provided two pieces of the deformable sleeve 15 a and 15 b . likewise , there is now provided two pieces of flexible tubing 13 a and 13 b . one piece of tubing may remain attached to a sampling device 25 such as the one shown in fig5 while the other piece of flexible tubing is removed from the sampling area with sample container 22 attached . once cut , the crimped flexible tubing 13 and deformable sleeve 15 form a seal 29 . due to the physical properties of the flexible tubing 13 and deformable sleeve 15 described in detail above , the flexible tubing 13 and deformable sleeve 15 remain substantially sealed thereby preventing fluid or other media from escaping the flexible tubing 13 . fig7 is an end view of a detachable transfer conduit 11 that has been cut or severed . in the cut state , the deformed or crimped sleeve 15 compresses the flexible tubing 13 to form a seal 29 . the detachable transfer conduit 11 may be assembled and then the entire device or components thereof may be rendered aseptic by , for example , gamma radiation . in another embodiment , the detachable transfer or components thereof may be rendered aseptic by exposure to steam above 121 ° c . for a period of time long enough to eliminate microorganisms . in yet another embodiment , the detachable transfer conduit or components thereof may be rendered aseptic by chemical treatment , such as with ethylene oxide ( eto ). in a preferred embodiment , the detachable transfer conduit is rendered aseptic by gamma radiation . once rendered aseptic , the detachable transfer conduit may be appropriately packaged and stored to maintain the aseptic state until ready for use . all dimensional information presented herein and included in the drawings is intended to be illustrative and not intended to limit the scope of the invention . the foregoing descriptions of detachable transfer conduit illustrate and describe various embodiments considered to represent best modes of carrying out the invention . as various changes can be made in the above embodiments without departing from the scope of the detachable transfer conduit disclosed and claimed herein , it is intended that all matter contained in the above description or shown in the accompanying drawings shall be interpreted as illustrative and not limiting . furthermore , the scope of the invention covers various modifications , combinations , alterations , etc ., of the above - described embodiments that all are within the scope of the claims . additionally , the disclosure shows and describes only selected embodiments of the invention , but the invention is capable of use in various other combinations , modifications , and environments and is capable of changes or modifications within the scope of the inventive concept as expressed herein , commensurate with the above teachings , and / or within the skill or knowledge of artisans in the relevant art . furthermore , certain features and characteristics of each embodiment may be selectively interchanged and applied to other illustrated and non - illustrated embodiments of the invention without departing from the scope of the invention .
a disconnect transfer conduit comprising flexible tubing ; a deformable sleeve surrounding a portion of the flexible tubing ; wherein the sleeve is attached to the flexible tubing and is formed of a material having plasticity such that pressure applied to the sleeve causes the sleeve to deform about and seal the flexible tubing and upon continued application of pressure to the sleeve , the sleeve and flexible tubing are cut and the sleeve retains a deformed shape substantially sealing the tubing . also provided is a method of sealing flexible tubing comprising applying pressure to a sleeve attached to flexible tubing to deform the sleeve and seal the tubing ; wherein , applying further pressure to the sleeve to cut the sleeve and tubing , thereby leaving the tubing substantially sealed where cut .