[ 0088 ] fig1 indicates that the cosmetic treatment induces a statistically significant reduction ( p & lt ; 0 . 05 ; bonferroni test ), equal to 33 % of the hairs removed by pull test with respect to the basal value , indicating an increase in resistance to traction . the data summarized in the charts of fig2 and 3 indicate that after a three - month period there occurred a statistically significant average increase ( p & lt ; 0 . 001 ; student &# 39 ; s test t ), equal to 13 % with respect to the basal value , in growing hairs evaluated by electronic counting . electronic counting of the number of hairs was performed by shaving an area of the scalp located on the vertex and measuring 1 cm 2 . in order to subsequently identify the shaving of the same region without error at the different times ( 1 , 2 , 3 months ), an area was determined with the aid of a cardboard cutout , starting from three coordinates constituted by the tip of the nose and the tip of the auricles . an electronic landmark was fixed on the images obtained for electronic counting . this area was photographed immediately after shaving and 5 days later . in this manner it is possible to electronically count the hairs in the active growth phase by computerized image analysis . the digitized images for electronic counting were acquired at the times t 0 a and t 3 a for each individual subjected to the cosmetic treatment . [ 0091 ] fig4 illustrates the morphological variations at the level of the bulb , observed by optical microscope . bulb analysis showed an increase in the bulbs in the anagen phase , i . e ., in the phase of new hair growth ( from 3 . 7 to 25 . 9 % of the total removed bulbs ) and a reduction in the bulbs in the telogen phase ( from 81 . 5 % to 57 . 7 % of the total removed bulbs ), which were statistically significant ( p & lt ; 0 . 05 ; student &# 39 ; s test t ). [ 0092 ] fig5 shows the variations observed also at the level of the shaft of the hair following the cosmetic treatment according to the invention . the variations at the level of the bulb and in the diameter of the shaft of the hair were determined by evaluation with an optical microscope . first of all , a region of the scalp adjacent to the thinning area was selected . then traction was applied until a sample composed of approximately ten hairs was obtained . the removed hair samples were collected in a transparent plastic bag . for microscopic analysis , the hairs were placed on a slide . if the hairs were longer than the slide , the part of the hairs that protruded from said slide was cut and then mounted on another slide . a second slide was placed on the first one and joined to it with adhesive tape at its ends . the shaft was examined along its entire length at low magnification (× 40 ) and at a higher magnification (× 100 and × 400 ), whereas bulb and end examination was performed at a higher magnification (× 100 and × 400 ). the following characteristics of the shaft were considered : state of the external sheath , uniformity and ruptures , if any , of the shaft . the characteristics of the bulb allowed to identify the phase of the hair ( anagen , telogen ). in the anagen phase , there is a dark end portion ( the bulb ) followed by a central dark long part ( the shaft ), surrounded by the internal sheath of the root . in the telogen phase , the end part of the shaft resembles a pad , the portion of the bulb is not pigmented and does not have the internal sheath of the root . the dystrophic bulb has a hook - like or deformed appearance and the sheaths may be absent , but it maintains the characteristic pigmentation of the anagen phase and is the result of traction during removal of the hair . [ 0095 ] fig6 shows that the cosmetic treatment determines , in subjects treated for three months , an increase in hair diameter which is significant from the cosmetic point of view . in particular , morphometric analysis of the hairs has shown an average increase of the diameter of 11 . 2 % with respect to basal conditions . the thickness of the shaft was measured by acquiring a computerized magnified image of the hair . [ 0096 ] fig7 shows that the brightness of the hairs of the individuals subjected to the treatment does not vary to a particularly appreciable extent . in order to perform sheen measurement , the l parameter according to the cie standard ( commission internationale de l &# 39 ; eclairage l a b ; 1976 ) was used as reference . [ 0097 ] fig8 shows that after three months of treatment there is a modest reduction in the amount of sebum produced by the sebaceous glands of the scalp . this reduction leads to an aesthetic improvement of the hair of the treated subject . the skin tester device ( ims , haifa ), constituted by an opaque glass surface mounted on a spring - loaded slider , was used to determine sebum values . the measurement was performed by resting the probe against the skin , by means of a pressure kept constant by the spring - loaded mechanism , for a few seconds and then returning the sensor to its seat . the apparatus is thus able to read the photometric variation in opaque surface transmittance after contact with the skin . the variation in the transparency of the surface of the probe is proportional to the amount of lipids present on the surface of the skin . the value obtained on the display of the device is inversely proportional to the amount of lipids collected by the probe . the cosmetic treatment according to the invention determines a statistically significant increase ( p & gt ; 0 . 05 ; bonferroni test ) in the level of cutaneous hydration , which is particularly evident when plotted as a bar chart ( fig9 ). the cutaneous hydration level was tested by using a cm820 comeometer ( courage - khazaka , köln , germany ) unit provided with a cylindrical sensor mounted on a spring - loaded slider and capable of performing capacitive electrical measurements . the measurements were performed by resting the sensor on the skin with a constant pressure which can be achieved thanks to the spring - loaded slider . the measurement principle is based on the fact that the dielectric constants of water and other materials are very different . a capacitor reacts with capacitance changes according to the water content when specimen tissues are placed within its measurement volume . the measurement of the capacitive properties of the surface of the skin is therefore an indirect expression of its degree of hydration . the value obtained on the display of the device is directly proportional to the water content of the surface of the skin . three types of formulation of the composition according to the invention , particularly suitable for topical application to male subjects , are given hereafter . the ingredients are provided by percentages by weight . ingredients i ii iii silanediol salicylate 0 . 042 0 . 042 0 . 042 glicoproteins 1 ppm 2 ppm 3 ppm zinc acetylmethionate 0 . 007 0 . 007 0 . 007 benzyl nicotinate 0 . 070 0 . 070 0 . 070 glutamine lysine hydrochloride 0 . 074 0 . 084 0 . 098 biotin sodium lauroyl cysteine 1 . 260 1 . 440 1 . 680 butylene glicol 0 . 151 0 . 151 0 . 151 glycerin 0 . 120 0 . 240 0 . 360 phosphatidylcholine 0 . 005 0 . 005 0 . 005 benzophenone - 4 0 . 080 0 . 080 0 . 080 alcohol denaturate 51 . 600 51 . 600 51 . 600 menthol 0 . 050 0 . 050 0 . 050 disodium edta 0 . 080 0 . 080 0 . 080 quaternium - 52 2 - bromo - 2 - nitropropane - 1 . 2 - diol 0 . 012 0 . 012 0 . 012 triethanolamine 0 . 032 0 . 032 0 . 032 cl 19140 , cl 14720 , cl 16255 , 0 . 001 0 . 001 0 . 001 cl 73015 , cl 28440 water the balance 100 . 00 100 . 00 100 . 00 three types of formulation of the composition according to the invention , particularly suitable for topical application to female subjects , are given hereafter . the ingredients are provided by percentages by weight . ingredients i ii iii silanediol salicylate 0 . 042 0 . 042 0 . 042 glicoproteins 1 ppm 2 ppm 3 ppm zinc acetylmethionate 0 . 007 0 . 007 0 . 007 benzyl nicotinate 0 . 070 0 . 070 0 . 070 glutamine 0 . 040 0 . 040 0 . 040 lysine hydrochloride 0 . 051 0 . 059 0 . 069 biotin 0 . 020 0 . 020 0 . 020 sodium lauroyl cysteine 0 . 882 1 . 008 1 . 176 butylene glicol 0 . 151 0 . 151 0 . 151 glycerin 0 . 120 0 . 240 0 . 360 phosphatidylcholine 0 . 005 0 . 005 0 . 005 benzophenone - 4 0 . 080 0 . 080 0 . 080 alcohol denaturate 51 . 600 51 . 600 51 . 600 menthol 0 . 050 0 . 050 0 . 050 disodium edta 0 . 080 0 . 080 0 . 080 quaternium - 52 0 . 100 0 . 100 0 . 100 2 - bromo - 2 - nitropropane - 1 . 2 - diol 0 . 012 0 . 012 0 . 012 triethanolamine 0 . 032 0 . 032 0 . 032 cl 19140 , cl 14720 , cl 16255 , 0 . 001 0 . 001 0 . 001 cl 73015 , cl 28440 water the balance 100 . 00 100 . 00 100 . 00 the activity of a preparation for the topical application according to example 1 was subjected to a clinical evaluation . this research has been carried out for evaluating the effectiveness of the preparation in promoting the physiological hair growth . a total of 10 men , aged 34 to 44 , affected by alopecia were selected and treated in double - blind conditions for a 12 weeks . a preparation containing the composition according to the invention was topically applied on a part ( side b ) of the scalp , whereas on the other side ( side a ) a lotion was applied which did not contain said active principle . the effectiveness was clinically evaluated by means of controls carried out during and at the end of the research . all the subjects showed a greater growth increase at the side of the scalp treated with the product under examination . it should be noted that the action of the product under examination takes place at the level of the hair follicles still active . the final evaluation made by the researcher for each subject of the test at the end of the treatment is as follows : subject age final evaluation i 36 very good result of the treatment . evident signs of growth , particularly at side b . ii 43 patient satisfied . thicker hair is objectively observed , particularly at side b . iii 41 good results of the treatment , particularly of that part of the scalp treated with lotion b . iv 38 treatment well tolerated , visible results of growth particularly at side b . v 36 treatment with good results : fairly good hair thickening , particularly with regard to side b . vi 44 very satisfied patient : good results both with regard to the hair loss and the signs of new growth . vii 35 treatment with good results : better growth . viii 34 extremely good results : loss within the physiological extent , growth signs ( more on side b ) found , patient extremely satisfied . ix 45 treatment with good results . growth signs present . x 35 very good results of treatment , both objective and subjective : little growth , better on side b . the disclosures in swiss patent application no . 1998 1330 / 98 from which this application claims priority are incorporated herein by reference .
the present invention concern a cosmetic composition for topical application for reactivating the physiological growth of hair , said composition comprising a synergistic association of cysteine , lysine or derivatives thereof , with a glycoprotein , in a physiologically acceptable vehicle .