neroli oil used in the present invention is obtained by steam distillation from flowers of citrus aurantium l . subsp . amara engel that is mainly produced in france , italy , spain , morocco and algeria . yield of the neroli oil is about 0 . 1 %. as the components contained therein , 1 - linalol and linalyl acetate ( the sum of these two ingredients are about 35 to 40 %), x - terpineol , geraniol , geranyl acetate , neloridol ( several %), and terpenes such as α - pinene , dipentene , camphene and ocimene , and sulfur - containing compounds such as methyl anthranilate and indole are mentioned . in the present invention , the oil obtained from citrus aurantium l . subsp . amara engel produced in france or spain is preferably used , because they contain geraniol and nerolidol . jasmine oil used in the present invention is obtained from jasminum officinale l . or j . officinale l . var . grandiflorum belonged to oleaceae family that is mainly grown in south france , italy , egypt or morocco . when the flower is extracted with solvent , concrete is obtained ; then the concrete is treated with alcohol to obtain jasmine absolute . as the components contained therein , benzyl acetate ( 65 %), d - linalol ( 15 . 5 %), linalyl acetate ( 7 . 5 %), jasmone ( inherent component ), methyl jasmonate ( inherent component ), jasmine lactone ( inherent component ), benzyl alcohol , methyl anthranilate , indol , nerol , geraniol and so forth are mentioned . in the present invention , jasmin produced in morocco is preferably used , because it contains jasmine lactone . lavender oil is obtained by steam distillation from flowers of lavandula officinalis chaix . which is mainly produced in france , italy , hungary , southern part of ex ussr , england , north america , australia , and hokkaido of japan . as the components contained therein , linalol ( 10 to 20 %), linalyl acetate ( 30 to 60 %), lavandulol , lavandulyl acetate , 3 - octanol , α - pinene , β - pinene , limonene , cineol , and citroneral and other components are mentioned . in the present invention , lavender produced in italy , hungary and france are preferably used , because it contains lavandulyl in relatively large amount . lavandin is obtained by steam distillation of the flower of lavandula hybrida reverch produced in south france . l . hybrida reverch . is generated by crossing lavender and spike lavender ( l . latifolia villars ). as the components contained therein , linalol , linalyl acetate , linalol oxide , cineol , d - campher , d - lavandulol and so forth are mentioned . in the present invention , lavandin produced in south france is preferably used , because it contains linalyl acetate and cineol in relatively large amount . rose oil is obtained from flower of damask rose , rosa damascena mill . form a trigintipetala dieck produced in bulgaria , turk , russia and , rosa centifolia l . produced in france , rosa damascena mill . produced in syria and morocco . at the last minutes of blooming , the rose is picked and immediately subject to steam distillation , thereby rose oil ( otto of rose , attar of rose ) is obtained in a yield from 0 . 01 to 0 . 04 . instead of steam distillation , when extraction with petroleum ether is performed , rose concrete is obtained in a yield from 0 . 22 to 0 . 25 %. from the concrete , absolute is obtained in the yield from 50 to 60 %. as the components contained therein , 1 - citronellol ( hereinbelow , “ l ” is sometimes described as “ l ”) and phenyletyl alcohol , main components , and geraniol , nerol , linalool , farnesol , rose oxide and damascenone , these two components are important components , and damascone , ionone , stearoptene , methyl eugenol , and the like . in the present invention , the rose absolute as described above is preferably used , because the quality of the essential oil and supply are stable . furthermore , that produced in france is more preferably , because it has high phenyletyl alcohol content . cineol type eucalyptus oil is obtained from leaves of eucalyptus globulus labill . by steam distillation , originally come from tasmania , is mainly gown in north america , mexico , africa , and south spain , in a yield from 0 . 75 to 1 . 25 %. as the components contained therein , cineol ( about 70 to 80 %), x - pinene , camphene , pinocarveol , pinocarvone , myrcenol , berebenone , carvone , eudesmol , and c 4 to c 6 aliphatic aldehyde , and the like are mentioned . in the present invention , the cineol type eucalyptus oil as describe above is preferably used , because it has high 1 , 8 - cineol content ; particularly , the cineol type eucalyptus oil produced in australia is stable in quality , as well as its quality is high and has good cost performance . as the essential oil describe above , commercially available ones supplied from ogawa & amp ; co ., ltd and so forth may be used . as a positive control in order to confirm the effect of the pharmaceutical agent for the diet of the present invention , grapefruit oil , lemon oil and sweet orange oil , which are known that they have diet effect and used , are solely used for preparing the pharmaceutical agent . grapefruit oil is obtained from pericarp of grapefruits , citrus paradisi macfayden that is mainly produced in california , florida , texas , israel and brazil . when pericarp of grapefruit is expressed , grapefruit oil is obtained , and when leaves or branches are subjected to steam distillation , grapefruit petitgrain oil is obtained . as the components contained therein , d - limonene at the ratio more than 90 %, nootkatone as the inherent component , octylaldehyde , citral , geraniol and acetate ester thereof are mentioned . for a reference example ; grapefruits oil that contains octyl aldehyde produced in california is preferably used . lemon oil is obtained by expression from pericarp of lemon , citrus limone ( l . ), which is mainly produced in california , sicily , calabria , spain and brazil as well as is cultivated in japan . it comprises , for example , d - limonene , citral , octyl aldehyde , nonyl aldehyde , linalol , geraniol , and other various terpenoid compounds . for a reference example , the essential oil from lemon that contains nonyl aldehyde produced in california is preferably used . sweet orange oil is obtained by expressing from fruits of citrus sinensis osbeck var . brasiliensis tanaka as a whole , which is grown in worldwide , for example , california , florida , spain , brazil , italy and japan , ant then separating the sweet orange oil and fruit juice . as the components contained therein , for example , limonene , citral , n - decylaldehyde , d - linalol , terpineol , and n - nonylalcohol are mentioned , and the content of d - limonene is more than 90 % for a reference example , the essential oil from sweet orange produced in california is preferably used , because of the n - decyl aldehyde contents . as the grapefruit oil , lemon oil , and sweet orange oil describe above , commercially available ones supplied from ogawa & amp ; co ., ltd and so forth may be used . l - menthol ( 5 - methyl 2 -( 1 - methylethyl ) cyclohexanol ) is usually called menthol ( hakkanou ). it has 12 isomeric forms in chemical , but only natural or synthetic l - menthol have cool aroma that is characteristic for menthol . l - menthol becomes a colorless column crystal or needle crystal soluble in ethanol , but insoluble in water . it gradually is sublimed at room temperature . in order to obtain natural menthol , mentha oil is cooled , and precipitated crystalline is separated by centrifugation . synthetic one is made of d - citronellal obtained from fractional distillation of citronella oil . d - citronellal is converted into l - isopulegol , and hydrogenated to obtain the synthesized menthol . alternatively , it is obtained by using either one method as described above : in one method , myrcene , which is obtained from pinene , is used as a raw material , to firstly obtain optically - active citronellal by using a specific catalyst , and menthol is asymmetrically synthesized , not performing an optical fractionation . on the other hand , menthol is also obtained by the optical fractionation of menthol mixture obtained from hydrogenated thymol . limonene ( p - mentha - 1 , 18 - diene ) is a monocyclic monoterpene hydrocarbon derived from menthane , and it has two enantiomers d - form and l - form , which is sometimes described as “ l - form ”. limonene is a liquid having lemon - like fragrance , and insoluble in water . in limonene , d - form is contained in orange peel oil , lemon oil , bergamot oil , fennel oil and the like ; l - form is contained in pine needle oil , mint oil and the like . a racemic form of limonene is called as dipentene , and it has high content in turpentine oil or camphor oil . d - limonene is obtained by steam distillation of pericarp of orange , lemon and the like , and then the obtained oil is subject to fractional distillation . l - limonene is obtained by fractional distillation of japanese mint oil , which is obtained from whole plants of japanese mint grown in hokkaido , okayama , brazil , paraguay , china and the like . dipentene is obtained by fractional distillation of camphor oil . the essential oil adsorbent used in the percutaneous type of the agent for diet of the present invention is defined as a resin to be a carrier for the essential oils as described above , and a polyvinyl alcohol ( pva ) type water - absorptive resin having the saponification value in the range of 98 . 0 to 98 . 5 is preferably used . if the saponification value is less than 98 . 0 , the surface of the carrier is gelatinized and loses functions as the adsorbent carrier . however , its saponification value is in the range of 98 . 0 to 98 . 5 , the surface is not gelatinized and maintains the functions as the adsorbent carrier . specifically , there are mentioned , for example , shin - etsu poval c - 17gp , poval a ( produced by shin - etsu chemical co ., ltd . ), and so forth , and shin - etsu poval c - 17gp or poval a is preferably used . the released water absorption agent used in the percutaneous type agent for diet of the present invention is defined as an absorption agent to remove the water existed on the skin on which the percutaneous type agent is applied . an acrylic type water - absorptive resin is preferably used , because such resin has high performance of water absorption in general , and also has good adhesive properties when it is subjected to a heating process for producing the composition described in below . the acrylic type water - absorptive resin may absorb water generated on the particular space of the skin surface during the percutaneous agent is applied on , and not limited to a particular resin . it is preferably used the resin being capable of absorbing 400 to 800 times of its volume of water based on the volume of the water - absorptive resin . if the capacity of the water - absorption is lower that 400 times , the resin is not capable of absorbing entire of the generated water , but the capacity over 800 times is not necessary . for example , there are mentioned sanfresh ( sanyo chemical industries . ltd . ), aquakeep ( registered trademark , sumitomo seika chemicals co ., ltd . ), and the like . among them , sanfresh having fractured form is preferably used because it has good adhesive property compared with aquakeep having particle form . an essential oil adsorbing and desorbing regulator used in the percutaneous pharmaceutical agent for diet of the present invention is defined as a porous carbon material that coats the surface of the layer formed by the above - mentioned essential oil on the essential oil adsorbent for regulating adsorption and desorption of the essential oil . specifically , there are mentioned , for example , activated charcoal that adsorbs a variety of molecules . the activated charcoal having 200 to 1 , 000 m 2 / g of surface area is preferably used ; because the amount of the essential oil adsorbed to the charcoal per weight is a little and thereby desorbing the essential oil becomes easy . the activated charcoal having 400 to 800 m 2 / g of surface area may be more preferably used . in the present invention , as the above - mentioned activated charcoal , which is finely divided particle , commercially available one may be used . there are mentioned , for example , shirasagi p ( takeda pharmaceutical company ltd .) and so forth . among them , shirasagi p is preferably used , because the adsorption area is not too large and the cost is reasonable . the base material for sheet forming used in the percutaneous type agent for diet the present invention is defined as the base material to form a sheet type diet composition . it contains an exothermic agent , a heat transfer inhibitor , an absorption promoter and a thermoplastic resin . the exothermic agent used in the percutaneous agent for diet of the present invention is defined as a material that adsorbs moisture in the air and then generates adsorption heat . by using the thermal energy generated when the moisture is adsorbed , the essential oil adsorbed onto the carrier adsorbent is desorbed . specifically , there is mentioned zeolite as an example . among the zeolite , synthetic one without metal and has pore size from 0 . 1 to 0 . 8 nm is preferably used , because the energy for adsorption and desorption is properly supplied . the zeolite has pore size from 0 . 3 to 0 . 4 nm is more preferably used . commercially available zeolite may be used when it has such pore sizes , and specifically , zeolum ( tosoh corporation ) and so forth are mentioned as examples . the heat transfer inhibitor is defined as a compound for inhibiting to transfer the heat , which is caused by the free - water adsorption onto the free - water adsorbent . specifically , there are mentioned , for example , polysaccharide compound such as chitosan and cellulose and so forth . when a dye is contained in the pharmaceutical preparation , use of chitosan gives an advantage that chitosan may be used as the carrier of such a dye . in order to inhibit the heat transfer , cellulose and the like are used instead of chitosan . the absorption promoter is defined as monoterpene that functions to improve to absorb the essential oil in the agent . as the absorption promoter , l - menthol and other terpene compound are specifically mentioned as the example , and commercially available one may be used . among them , l - menthol has the advantageous effect that it removes the free - water remained on the skin , and then arranges circumstances for the essential oil to be absorbed through the skin . the thermoplastic resin having the saponification value , about 88 . 0 , is preferably used . in order to form the percutaneous agent for diet , the composition for the agent should be subjected to a heating process . in this time , the resin should have preferable properties that neither release the essential oils from the carbon coated particle nor stuck spaces among the resin particle , even though the adhesion is performed under lower temperature , such as about 180 ° c . specifically , for example , gohselan l - 0301 ( registered trademark , nippon synthetic chemical industry co ., ltd .) is preferably used , because of the good adhesive properties at low temperature about 180 ° c . the percutaneous agent for diet of the present invention may be prepared as plaster form agent after mixing the essential oil - coated particles , and then the particles are mixed with hydrophobic oleaginous base used for preparing ointment or suppository , or hydrophilic base easily soluble in water among the base defined in the japanese pharmacopoeia as the oleaginous base , there are mentioned , for example , lard , beef tallow , fatty oil , hydrocarbons , higher alcohols , higher fatty acid , higher fatty acid esters , glycols , plant oils , animal oils and so forth . among them , as the hydrocarbon , there are mentioned , for example , liquid paraffin that is a mixture of a variety of the hydrocarbons , paraffin having branched chain ( commercial name , isopar , registered trademark , exxon mobil corporation ), solid paraffin , white soft paraffin , and so forth . as the higher fatty acid , there are mentioned , for example , caproic acid , enanthic acid , caprylic acid , pelargonic acid , undecylic acid , lauric acid , tridecylic acid , myristic acid , pentadecylic acid , palmitic acid , heptadecylic acid , stearic acid , oleic acid , and so forth . as the higher fatty acid ester , there are mentioned , for example , fatty acid ester such as myristyl myristate , myristyl palmitate , stearyl stearate , ceryl lignocerate : lanolin ; lately describing waxes ; glyceryl laurate , glyceryl monomyristate , glyceryl monooleate , glyceryl monostearate , glyceryl dilaurate , glyceryl dimyristate , glyceryl distearate , glyceryl trilaurate , glyceryl trimyristate , glyceryl tristearate , and so forth . as the fatty oil , there are mentioned , for example , the plant oil such as soybean oil , camellia oil , rape seed oil , peanut oil , sesame oil , and safflower oil ; animal oil such as mink oil , egg yolk oil , squarane , fish oil , whale oil , and liver oil ; and hardened oil produced by hydrogenation thereof is also mentioned . as the wax , there are mentioned , for example , carnauba wax , yellow bee wax , white beeswax , and so forth . as the petrolatum , there are mentioned , for example , yellow petrolatum , white soft paraffin and so forth . white soft paraffin is preferably used , because it is compatible to almost pharmaceutical drug with no change . according to the description on the japan pharmacopoeia , white soft paraffin is combined with white bee wax and sorbitan sesquioleate to prepare white ointment and then absorption promoter is properly added to prepare the plaster . alternatively , as the water soluble base , there are mentioned , for example , carboxyvinylpolymer , hydroxypropylcellulose ( herein below , it is also referred to as simply “ hpc ”), macrogol , methylcellulose , and so forth . a commercially available water - soluble base , hiviswako ( registered trademark , wako pure chemical industries , ltd .) is preferably used . when polyethylene glycol 400 and polyethylene glycol 4000 are mixed at equal ratio , the macrogol ointment is prepared and may be used as the water - soluble base . alternatively , hpc having good compatibility with propylene glycol may be used as the base . note that skin irritation is reduced by adding , at least , diisopropyl - ethanolamine and diisopropyladipate as neutralization agents , when an acidic water - soluble polymer is used as the base . since the main ingredient of the present invention is aliphatic essential oils obtained from plants , for example , l - menthol or limonene may be preferably used , are combined with oleaginous base , if necessary . when the main ingredient is combined with the water - soluble base , the absorption promoter is not necessary . however , menthol may be used in the amount as mentioned above . the percutaneous agent for diet of the present invention is prepared by using the essential oil , the essential oil adsorbent , the free - water remover , the essential oil adsorbing and desorbing regulator , the exothermic agent , the heat transfer inhibitor , the absorption promoter , and the base material for sheet forming , as described above . the method for producing thereof is explained in below . at first , a desired amount of the essential oil is weighed , and then it is mixed with pva to coat the surface of pva particle in a proper size vessel . subsequently , the desired amount of the activated charcoal is weighed and added to cover the surface of the essential oil - coated pva to prepare the charcoal - coated particle . next , zeolum which is the exothermic agent , chitosan which is the heat transfer inhibitor , l - menthol which is the absorption promoter , sanfresh which is the base material for sheet forming , and activated charcoal are homogenously mixed in the desirable ratio to prepare a base for the agent . wherein , in order to improve formability of the sheet type composition lately described , the additional activated charcoal is added separately from those used for forming the carbon - coated particles . after that , the mixture is spread out on a sheet for contact bonding and then another sheet for contact bonding covers them to be heated to prepare the sheet type of the composition for the percutaneous agent for the diet of the present invention . after the mixture is sandwiched by the sheets for contact bonding , the sandwiched mixture is preferably heated from about 160 ° c . to about 200 ° c ., more preferably about 180 ° c . by heating the sandwiched mixture at the temperature of the above - mentioned range , the base material for sheet forming , the resin , is adhered each other , keeping spaces among them . since the spaces works as isles for the molecule of the essential oil from the charcoal coated particle to the skin surface , on which the percutaneous anxiolytic of the present invention is applied , the molecule of the essential oil is delivered to the skin and absorbed percutaneously . on the other hand , when the mixture is heated about 230 ° c . or higher temperature than it , the spaces among the resin are filled , because the resins are melted . therefore , the molecule of the essential oil described above is incapable of delivering to the skin . as the sheet for contact bonding , kasenshi paper ( basis weight 18 to 20 g ) is preferably used , and the use of kasenshi paper has the advantage that the ratio of contact bonding of carbon coated particles is high . the sheets contact bonded to the composition is sandwiched with two sheet type materials made of nonwoven fabrics with proper sizes , for example , 2 × 4 cm ; and then four sides of the sheets are heat - sealed to prepare a piece of the percutaneous agent for diet of the present invention . alternatively , the sheet type material is preferably selected from the group consisting of paper , woven fabric and nonwoven fabric . among them , the nonwoven fabric is more preferable because of its good permeability . the essential oil contained in the percutaneous agent is released as gaseous state , and it flows in the spaces among the particles of the base material for sheet forming to go out by permeating the fabric . plaster agents are prepared in below . at first , the essential oils are weighed at the desirable amounts , and mixed with pva to coat the surface of pva . after that , pva with the essential oil coated is mixed with the desirable amount of the activated charcoal as described above to form the carbon - coated particle a . subsequently , the desirable amount of menthol is weighed , and it is refereed to as the particle b . these particles a and b are mixed , and after the addition of menthol , the mixture is further mixed with the oleaginous base or the water - soluble base , and spread on a sheet made of the paper , the plastic film , and so forth . as explained above , the composition for the diet and the agent for diet of the present invention are prepared . alternatively , as the reference examples , three agents including sole essential oil instead of the above - mentioned combination of the three carbon - coated particles but others , are prepared as the same as previously described . in this way , the reference agents may be prepared . note that the sheet type percutaneous type agent and the plaster are mentioned in the above - described embodiment . however , the agent is prepared as an ointment by using generally used base for preparing the ointment with the essential oil coated particles and absorption promoter , or cream and so forth . the present invention will now be described by the following examples in detail . however , the present invention is not limited to the examples . in order to produce the composition for the percutaneous type agent for diet and the percutaneous agent ( the examples ), positive control agent ( the reference examples ), and placebo ( the comparative examples ), the following reagents were used . neroli oil , jasmine oil , lavender oil , rose oil , cineol type eucalyptus oil , and l - menthol is purchased from ogawa & amp ; co ., ltd . grapefruits oil , lemon oil and sweet orange oil are also purchased from ogawa & amp ; co ., ltd . as pva , shin - etsu poval c - 17gp was purchased from shin - etsu chemical co ., ltd ., and goselan l - 3031 was purchased from nippon synthetic chemical industry co ., ltd . as acrylic type of water - absorptive . resin , sanfresh ( registered trademark ) was purchased from sanyo chemical industries , ltd . as activated charcoal , shirasagi p was purchased from takeda pharmaceutical company limited . as zeolite , zeolum ( registered trademark ) is purchased from tosoh corporation . as chitosan , koyo chitosan was purchased from koyo chemical company limited . kasenshi paper is purchased from nippon daishowa paperboard co ., ltd ., and the non - woven fabric is purchased from nisshinbo industries , inc . the carbon coated particle was prepared according to the recipe shown in the following table 1 . note that each piece of the agent was regulated so as to contain 1 . 1 mg of each carbon - coated particle prepared by using the amount shown in the table 1 , when the pharmaceutical agents ( name of the agent is obt - a , obt - b , and obt - c ) were prepared . for example , in the combination a , neroli oil ( n ), jasmine oil ( j ) and lavender oil ( l ) were separately weighed at the amount shown in the table 3 , and put them separately in three different 500 ml of a transparent closed vessel made of glass . pva ( shin - etsu poval c - 17gp ) was added into the vessel and mixed with each essential oil at the room temperature to prepare the essential oil - coated particles . the carbon coated particles prepared as mentioned above were then stored in different tight sealed vessels separately , for example , a glass vessel with a ground - glass stopper , at room temperature . then , each of the carbon - coated particles was separately weighed in the amount mentioned in the table 3 to prepare the combination of the particles a , b and c . these a , b , and c were corresponding to the name of the agent obt - a , obt - b , and obt - c . alternatively , the base described in the following table 4 was prepared in parallel with the preparation of the combination of the carbon - coated particles a , b , and c as described above . three kinds of carbon - coated particles prepared in the above ( 2 - 1 ) were mixed with the base and other components of the amounts shown in table 4 to prepare the percutaneous type agent for diet (× 1 ). namely , the combination of the essential oil - coated particles a , b , and c shown in table 3 , the base shown in table 5 , and activated charcoal for forming , chitosan for forming , and pva were mixed in the amounts shown in table 5 , and prepared each of the mixture , obt - a , obt - b , or obt - c of the agent of the present invention . subsequently , the composition was spread on the sheet for contact bonding so as to have even thickness , and then another sheet for contact bonding was placed on them to sandwich the mixture . the sandwiched mixture is then subjected to contact bonding under heating , about 180 ° c ., and formed as the sheet type composition for diet agent . the sheet type composition was cut in a size of about 2 × 4 cm , or about 1 × 2 cm to form pieces and sandwiched with the nonwoven fabric that is also cut in the proper size to cover the piece . after that , four sides of the nonwoven fabric was heat sealed to prepare the percutaneous agent for the diet . the recipe including the combination of the carbon coated particle a shown in table 3 was named as obt - a , including that of b was named as obt - b , and including that of c was named as obt - c , respectively . note that the loss rate of the essential oil during the contact bonding described later was calculated as 15 %. except including the combination of the carbon coated particle in the amounts shown in tables 6 and 7 , the percutaneous type agents for the diet including the double amount of the active ingredient were prepared similarly to the above ( 1 ). by using grapefruits oil , lemon oil , or sweet orange oil , according to the protocol as described above , the carbon - coated particles corresponding to the essential oils used were prepared . the agent , which solely includes one of the carbon - coated particle was used instead of the combination of the carbon - coated particles , was prepared as reference examples . the agent including the carbon coated particles of grapefruits was names as ob - a , that of lemon oil was named as ob - b , and that of sweet orange oil was named as ob - c . the carbon coated particles included in three reference example agents and their composition were shown in the following table 8 and 9 . except including the combination of the carbon coated particle in the amounts shown in tables 10 and 11 , the percutaneous type agents for the diet including the double amount of the active ingredient (× 2 ) were prepared similarly to the above ( 1 ). placebo was prepared as the same as mentioned above , using black paper not including essential oil coating particles instead of the sheet type composition of the present invention . evaluation of the diet effect by each percutaneous agent including essential oils evaluation of the diet by the percutaneous agents including each essential oils was performed by using two indexes : weight change of the panniculus adiposus of the abodomen and level change of leptin in plasma . as the test animals , 5 weeks age of icr mice were purchased from tokyo laboratory animals co . ltd . these mice were kept under the conditions 10 mice per cage , at 24 ± 1 ° c ., 12 hours light - dark cycle ( turn on the lights at 8 o &# 39 ; clock and turn off the lights at 20 : 00 o &# 39 ; clock ) for 1 week , taking fed and water freely . after that , the agents for the diet of the present invention ( examples ), the reference agents ( reference example ), and placebo agents were assigned to different cages , and these agents were adhered for 14 days repeatedly . pentobarbital was purchased from dainippon pharmaceutical co ., ltd . in order to determine the leptin level in the plasma , leptin / mouse elisa kit was purchased from morinaga institute of biological science inc . on the beginning day of adhesion of the agents , the mice were anesthetized by using pentobarbital ( 70 mg / kg , i . p . ), and then shaved hair around waistline . on the same day , the agent for diet , the reference agent , or the placebo agent was adhered on the every mice of each group as assigned under right ether anesthesia . these agents adhered were replaced to new agents every day , for 14 days . after 24 hour from the final adhesion , the mice were decapitated to collect blood and the panniculus adiposus of the abodomen . the blood was collected from the cervical part decapitated into eppendorf tube ( 1 . 5 ml of volume size ) in which its inner surface was wetted by heparin solution . the tube was immediately turned upside down to prepare blood samples . then , the blood samples were centrifuged for 15 minutes at 10 , 000 × g at 4 ° c ., and the separated plasma were stored in a frozen state in a freezer at − 80 ° c . by using the mouse / leptin elisa kit , the leptin levels in the plasma samples were determined . the panniculus adiposus of the abodomen was collected by removing the adiposus around inguinal area of both legs . two adipose tissues obtained form each mouse were weighed together . obtained data were subjected to comparison between groups : the placebo agent adhesion group and the reference example agent adhesion group , the placebo agent adhesion group and the diet agent adhesion group . in order to confirm dispersion , the data was analyzed by f - test . if the significant difference was not less than 5 %, the data was analyzed by using student t - test for further evaluation ; if it is less than 5 %, the data was analyzed by using aspin - welch t - test for further evaluation . when p & lt ; 0 . 05 in these two t - test , it was decided that there was significant difference between two groups compared , and showed by asterisk in fig1 and 2 . the effect to the leptin level in the plasma was shown in fig1 . in fig1 , “ 1 ” shows the standard of each agent and “ 2 ” shows these including double amounts of the standard . as shown in fig1 , the significant decrease of the leptin level in plasma was observed only when the double amounts of ob - b (× 2 ) was adhered . on the other hand , the standard of obt - a (× 1 ), the double amount of it (× 2 ), and the double amounts of obt - b (× 2 ) showed the significant decrease . it was demonstrated that these agents have effect to decrease plasma leptin level . effect for the panniculus adiposus tissues of the abdomen was shown in fig2 . in fig2 , “ 1 ” shows the standard of each agent and “ 2 ” shows these including double amounts of the standard . as shown in fig2 , the significant decrease was observed only when the double amounts of ob - b (× 2 ) was adhered similarly to the plasma leptin level . on the other hand , no significant decrease of the weight of the panniculus adiposus by the standard of obt - a was observed , although it showed the significant decrease of the plasma leptin level . however , the double amounts of obt - a significantly decreased the panniculus adiposus . in obt - b , the significant decrease of the weight of the panniculus adiposus of the abdomen by either of the standard or the double amounts . furthermore , even in obt - c , which did not show the significant decrease of the plasma leptin level , the weight of the panniculus adiposus of the abdomen was significantly decreased when the double amounts of obt - c was adhered . accordingly , it was demonstrated that obt - a to obt - c had the advantageous effect to decrease the plasma leptin level or the weight of panniculus adiposus of the abdomen , comparing to obt - a to obt - c , which include one essential oil . diet effect for obesity mice caused by eating high - fat feeding staff as the test animals , 4 weeks age of icr mice were purchased from tokyo laboratory animals co . ltd . these mice were kept under the conditions 10 mice per cage , at 24 ± 1 ° c ., 12 hours light - dark cycle ( turn on the lights at 8 o &# 39 ; clock and turn off the lights at 20 : 00 o &# 39 ; clock ) for 1 week , taking fed and water freely . as a high - fat feeding staff , high fat diet 32 was purchased from crea japan inc , and as a normal feeding staff , mf was purchased from oriental yeast co ., ltd . pentobarbital was purchased from dainippon pharmaceutical co ., ltd . in order to determine the leptin level in the plasma , leptin / mouse elisa kit was purchased from morinaga institute of biological science inc . as the agent for diet of the present invention , as mentioned above , the standard of obt - a ( obt - a × 1 ) and the double amounts of it ( obt - a × 2 ), the standard of obt - b ( obt - b × 1 ) and the double amounts of it ( obt - b × 2 ) were used . as the placebo agent , the control agent shown in the ( example 2 - 4 ) was used . as a test group , icr mice described above was used after being fed the high - fat feeding staff for 4 weeks and induced the adipositas . the mice were fed either of the above - mentioned feeding staff for 4 weeks , and then they received each of the agents for the diet by adhesion . the groups were named the test group a × 1 , a × 2 , b × 1 , and b × 2 . the negative control group was gave the normal feeding staff and the control agent by adhesion , and the positive control group gave the high - fat feeding staff and the control agent by adhesion . all of the mice of the groups were weighed everyday . on day 28 from beginning of feeding either of the feeding staff to the mice , the mice were anesthetized by using pentobarbital ( 70 mg / kg , i . p . ), and then shaved their hair around waistline . the agents were adhered on shaved site of the mice under right ether anesthesia . these agents adhered were replaced to new agents every day , for 14 days . after 24 hour from the final adhesion of the agent , the mice were decapitated to collect blood and the panniculus adiposus of the abodomen . the blood was immediately turned upside down in the tube , and centrifuged for 15 minutes at 10 , 000 × g at 4 ° c . to separate , and the separated plasma were stored in the frozen state in a freezer at − 80 ° c . as the sample . the plasma leptin level was determined by using the mouse / leptin elisa kit similarly to the example 3 . the panniculus adiposus tissues were weighed . as shown in fig3 , after 14 day from the beginning of feeding , the significant difference was observed between the normal feeding staff group and the high - fat feeding staff group . as shown in fig4 , the adipose weight of the abdomen was significantly different between the negative control group and the positive control group . all of the mice in the agents for the diet adhered group showed lower weight compared to that of the mice in the positive control group . in fig4 , the group shows significant difference was gave asterisk ( p & lt ; 0 . 05 ). as shown in fig5 , the same tendency was observed in the plasma leptin level . in b × 1 group and b × 2 group , there were no significant difference compared to the positive control group , and between b × 1 and b × 2 groups . between the positive control group and a × 1 group , there was no significant difference . however , between the positive control group and a × 2 group , there was significant difference , and a × 2 suppressed the increase of the plasma leptin level significantly ( p & lt ; 0 . 05 ). in fig5 , the group shows significant difference was gave asterisk or #. as a result , it was demonstrated that the double amounts of obt - a showed the decrease of plasma leptin level or weight of panniculus adiposus of the abdomen , even in the adipositas was induced by feeding the high - fat feeding staff . according to the agent for diet of the present invention , it is available in the medical field related to obesity prevention or treatment by decreasing the plasma leptin level or loosing weight of panniculus adiposus .
the present invention provides a composition for diet comprising an essential oil adsorbent is coated with neroli oil to prepare an essential oil - coated particle , and then the particle is covered with a essential oil adsorbing and desorbing agent to prepare the carbon - coated particle , the carbon - coated particle similarly prepared by using jasmin oil instead of neroli oil , the carbon - coated particle similarly prepared by using rose oil instead of neroli oil , and / or the carbon - coated particle similarly prepared by using cineol type eucalyptus oil instead of neroli oil , and the agent for diet comprising thereof as active ingredients . accordingly , the agent for treating adipositas having high safety , of which composition ratio can be changed depending on a symptom of adipositas , is provided .