the compound cpt - 11 is also known as irinotecan and is sold under the trade name camptosar ®. cpt - 11 is a chemotherapy drug used to treat patients with advanced cancer of the large intestine and colon . it is administered periodically by bolus or infusion injection at a dose of 120 - 180 mg / m 2 during six week treatment cycles . cpt - 11 is typically administered in combination with 5 - fluorouracil ( 5 - fu ) and leucovorin ( lv ). the compound pemetrexed is sold under the trade name alimta ®. pemetrexed is a chemotherapy drug used to treat patients with locally advanced or metastatic non - small cell lung cancer after prior chemotherapy . pemetrexed in combination with cisplatin is indicated for the treatment of patients with malignant pleural mesothelioma whose disease is unresectable or who are otherwise not candidates for curative surgery . typically 500 mg / m 2 of pemetrexed is administered to a patient by infusion over 10 minutes every 21 days after pre - treatment with folic acid , vitamin b 12 and dexamethasone . the term “ platinum chemotherapeutic agent ” is taken to mean a cancer chemotherapeutic agent that contains platinum . specific platinum chemotherapeutic agents contemplated by the method of this invention include cisplatin , carboplatin , and oxaliplatin . the use of cisplatin or carboplatin is preferred . the compound cisplatin is sold under the trade name platinol ®- aq . cisplatin is administered to treat patients with metastatic ovarian tumors who have already received appropriate surgical and / or radiotherapeutic procedures . as a single agent , cisplatin is typically administered at a dose of 100 mg / m 2 iv per cycle , once every four weeks . cisplatin may also be administered in combination with cytoxan ®. the compound carboplatin is sold under the trade name paraplatin ®. carboplatin is administered to treat patients with ovarian carcinoma . as a single agent , carboplatin is typically administered at a dose of 360 mg / m 2 iv per cycle , once every four weeks . carboplatin may also be administered with cyclophosphamide . the compound oxaliplatin is sold under the trade name eloxatin ®. oxaliplatin is administered to treat patients with colorectal cancer . it is typically administered at a dose of 85 mg / m 2 iv per cycle , once every two weeks in combination with 5 - fu and lv . the compound doxorubicin is sold under the trade names adriamycin ® and rubex ®. doxorubicin has been used successfully to produce regression in disseminated neoplastic conditions such as acute lymphoblastic leukemia , acute myeloblastic leukemia , wilms &# 39 ; tumor , neuroblastoma , soft tissue and bone sarcomas , breast carcinoma , ovarian carcinoma , transitional cell bladder carcinoma , thyroid carcinoma , gastric carcinoma , hodgkin &# 39 ; s disease , malignant lymphoma and bronchogenic carcinoma . it is typically administered at a dose of 60 - 75 mg / m 2 iv at 21 - day intervals . the compound etoposide is sold under the trade names etopophos ®, toposar ® and vepesid ®. etoposide is administered to treat patients with testicular or lung cancer . it is typically administered by injection at a dose of from 5 - 100 mg / m 2 . the compound 5 - fluorouracil ( 5 - fu ) is sold under the trade name adrucil ®. it is administered to treat patients with carcinoma of the colon , rectum , breast , stomach and pancreas . 5 - fu is typically administered iv at a dose of 12 mg / kg once daily for four successive days . gemcitabine is sold under the trade name gemzar ®. it is most commonly used to treat non - small cell lung cancer , pancreatic cancer , bladder cancer , and breast cancer . it is typically administered by iv infusion at a dose of 1000 mg / m 2 over 30 minutes weekly for 3 consecutive weeks out of 4 weeks . the skilled artisan will appreciate that the exact dosage and number of treatment cycles of any of the agents described above required to treat a patient are determined by a physician in view of the stage and severity of the disease as well as the specific needs and response of an individual patient . the compound 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine is taught to be an inhibitor of gsk - 3β in wo 03 / 076442 , where it is referred to as 3 -( 9 - fluoro - 6 -( piperidin - 1 - yl ) carbonyl )- 6 , 7 - dihydro - 6h -[ 1 , 4 ] diazepino -[ 6 , 7 , 1 - hi ] indol - 1 - yl )- 4 -( imidazo [ 1 , 2 - a ] pyridin - 3 - yl )- 2 , 5 - dioxopyrrole ( example 365 , page 113 ). the two naming conventions described above are taken to be synonymous and each is taken to identify the following structure : compound i is a base , and accordingly may react with any of a number of inorganic and organic acids to form pharmaceutically acceptable acid addition salts . preferred pharmaceutically acceptable salts are those formed with hcl , hbr , sulfuric acid , or methanesulfonic acid . compound i forms solvates with , for example , water , methanol , and ethanol . a preferred solvate is that formed with ethanol . although compound i lacks useful antitumor activity in its own right , when administered in combination with cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , or platinum chemotherapeutic agents , a significant therapeutic benefit in the treatment of ovarian , non - small cell lung , or colorectal cancer is realized relative to treatment with cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , or platinum chemotherapeutic agents alone . one benefit of this combination is that the therapeutic effect of cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , and platinum chemotherapeutic agents is potentiated by co - administration with compound i . that is , a lower dose of cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , or platinum chemotherapeutic agents than is typically administered will provide a similar therapeutic effect to the patient . furthermore , a greater therapeutic effect of cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , or platinum chemotherapeutic agents will be realized by the patient at the typical dose when these agents are co - administered with compound i . this method of treatment provides the further advantage of convenience for the patient and physician , allowing administration of compound i in the same treatment schedule as cpt - 11 , pemetrexed , gemcitabine , etoposide , doxorubicin , or platinum chemotherapeutic agents . the combination therapy of this invention is an improved method of treating a patient suffering from ovarian cancer , non - small cell lung cancer , or colorectal cancer . the patient is a mammal and the preferred mammal is a human . although all of the described combinations of compound i or a pharmaceutically acceptable salt or solvate thereof and chemotherapeutic agents are useful , certain combinations are preferred . one preferred combination is the co - administration of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine or a pharmaceutically acceptable salt or solvate thereof with a platinum chemotherapeutic agent . another embodiment is the co - administration of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine or a pharmaceutically acceptable salt or solvate thereof with either cisplatin or carboplatin . a further embodiment of the invention is the administration of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine or a pharmaceutically acceptable salt or solvate thereof with pemetrexed and a platinum chemotherapeutic agent . it is preferred that 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine or a pharmaceutically acceptable salt or solvate thereof be administered with pemetrexed and carboplatin . the phrase “ an effective amount of a chemotherapeutic agent ” is taken to mean the dosage of the particular chemotherapeutic agent necessary to either destroy the target cancer cells or slow or arrest the progression of the cancer in a patient when the chemotherapeutic agent is administered in combination with compound i or a pharmaceutically acceptable salt or a solvate thereof . the phrase “ an effective amount of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinylcarbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine or a pharmaceutically acceptable salt or a solvate thereof ” is taken to mean the dosage of compound i or a pharmaceutically acceptable salt or a solvate thereof necessary to potentiate the effect of a specific dose of a particular chemotherapeutic agent in order to either destroy the target cancer cells or slow or arrest the progression of the cancer in a patient . anticipated dosages of compound i or a pharmaceutically acceptable salt or a solvate thereof are in the range of 5 to 600 mg / patient / day . preferred dosages are in the range of 50 to 400 mg / patient / day . most preferred dosages are in the range of 100 to 400 mg / patient / day . the exact dosage required to treat a patient will be determined by a physician in view of the stage and severity of the disease as well as the specific needs and response of the individual patient . the phrase “ to potentiate the effect of a specific dose of a particular chemotherapeutic agent ” is taken to mean that a lower dose of a chemotherapeutic agent than is typically administered will be an effective dose , or that a greater therapeutic effect of the chemotherapeutic agent is realized by the patient at the typical dose when it is co - administered with compound i or a pharmaceutically acceptable salt or solvate thereof . the terms “ co - administered ” and “ co - administration ” as well as the phrases “ in combination with ” and “ administered in combination with ” as used herein are taken to mean that compound i or a pharmaceutically acceptable salt or solvate thereof is given to the patient during the same treatment cycle as cpt - 11 , pemetrexed , or a platinum chemotherapeutic agent . that is , compound i or a pharmaceutically acceptable salt or solvate thereof may be administered prior to , during , or after the administration of cpt - 11 , pemetrexed , or a platinum chemotherapeutic agent at the discretion of the physician taking into account the tumor type , the stage of the disease , the specific chemotherapeutic agent employed , and the condition and sensitivity of the patient . the following in vitro and in vivo studies demonstrate the advantages of these combinations . apoptosis or programmed cell death is characterized by a set of biochemical reactions , one of which is the induction of caspases . activated caspases are proteases that participate in a cascade of cleavage events that disable key enzymes responsible for cell homeostasis and repair . caspases 3 and 7 play key effector roles in apoptosis and can be detected and measured by a fluorescent biochemical assay . the increase of caspase - 3 / 7 activity in cells is directly correlated to apoptotic activity . ( d . w . nicholson , et al ., nature , 376 , 37 - 43 ( 1995 )) the promega apo - one homogeneous caspase - 3 / 7 assay kit was used ( catalog # g7791 ). the assay buffer consists of 30 mm hepes ( n -( 2 - hydroxyethyl ) piperazine - n ′-( 2 - ethanesulfonic acid ) ph 7 . 4 , 150 mm nacl , 50 mm kcl , 10 mm mgcl 2 , 0 . 4 mm egta ( ethylene glycol tetraacetic acid ), 0 . 5 % nonidet p40 ( octylphenolpoly ( ethyleneglycol ether )), 0 . 1 % chaps ( 3 -[( 3 - cholamidopropyl ) dimeth - ylammonio ]- 1 - propanesulfonate hydrate and 10 % sucrose , which lyses / permeabilizes cultured cells and a caspase 3 / 7 substrate , z - devd ( z - asp ( ome )- glu ( ome )- val - asp ( ome )), coupled to a profluorescent rhodamine 110 . when the buffer - substrate mixture is added to a test sample , the cleavage and subsequent removal of the devd peptides by caspase 3 / 7 activity results in intense fluorescence of the rhodamine 110 leaving group , which is detected by excitation at 499 nm . the amount of fluorescent product is proportional to the amount of caspase 3 / 7 cleavage activity in the sample . to measure the apoptotic effect of test compounds , tumor cells are plated at 1 × 10 4 cells per well in 96 well plates and incubated overnight at 37 ° c ., with 5 % co 2 . tumor cells are treated with test compound at desired concentrations in triplicate , including untreated / negative control wells . the assay plates are re - incubated for 48 hrs . at the end of the incubation period , a mixture of the assay buffer and substrate is added to each sample well . the fluorescence in each well is measured at an excitation wavelength of 485 +/− 20 nm and an emission wavelength of 530 +/− 25 nm . the % increase of caspase activity in treated cells is calculated relative to untreated controls . hct - 116 and colo - 205 are colorectal carcinomas , a2780 and skov3 are ovarian carcinomas , a549 , calu - 6 , and nci h - 460 are non - small cell lung carcinomas , and ags , kato iii , and mkn 45 are gastric carcinomas . in the following tables the term “ compound i ” or “ cmpd i ” are taken to mean 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine . the data in tables 1 - 12 are expressed as % increase of caspase activity relative to untreated controls unless otherwise noted . the fold increase data in tables 1 - 12 reflects the potentiation of chemotherapeutic drug - mediated apoptosis in cells by the co - administration of compound i relative to the apoptotic effect of the chemotherapeutic drug alone . cultured cells are implanted subcutaneously in the rear flank of female cd - 1 nu / nu strain mice which have been acclimated for one week in the animal facility after receipt from the vendor . mice are randomized into groups of 7 or 8 mice per group and treatment begun when the mean tumor volume reaches ˜ 100 mm 3 compound i is dosed iv and the chemotherapeutic agent is given ip . when the agents are given in combination , the chemotherapeutic agent is dosed 60 minutes prior to compound i . the tumors are measured 2 times per week by electronic calipers to plot growth curves . tumor growth delay is the increase in median time it takes for a tumor to reach 1000 mm 3 in volume when compared to a control group . animals are also monitored for fluctuations in body weight and survival . 5 mg / kg cisplatin is dosed ip alone and in combination with 5 mg / kg compound i ( injected iv ). compound i at 5 mg / kg ( injected iv ), is also dosed alone as a control group . animals received 3 consecutive cycles of cisplatin and compound i , each cycle separated by 7 days . 10 mg / kg cisplatin is dosed ip alone and in combination with 5 mg / kg compound i ( injected iv ). compound i at 5 mg / kg ( injected iv ), is also dosed alone as a control group . animals received 3 consecutive cycles of cisplatin and ly2090314 , each cycle separated by 7 days . 5 mg / kg cisplatin is dosed ip alone and each in combination with 5 mg / kg compound i ( injected iv ). compound i at 5 mg / kg ( injected iv ), is also dosed alone as a control group . animals received 3 consecutive cycles of cisplatin and compound i , each cycle separated by 7 days . compound i is dosed iv at 5 mg / kg alone and with 50 mg / kg carboplatin ip . dosing is every 14 days × 3 cycles . for treatment groups receiving both compound i and carboplatin , carboplatin is administered 60 minutes prior to compound i . compound i is dosed iv at 5 mg / kg alone , with 10 mg / kg carboplatin ip , with 300 mg / kg pemetrexed ip , and with 10 mg / kg carboplatin ip and 300 mg / kg pemetrexed ip . dosing is every 14 days for 3 cycles . for treatment groups receiving both compound i and carboplatin , carboplatin is administered 60 minutes prior to compound i . for treatment groups receiving both compound i and pemetrexed , pemetrexed is administered 24 hours prior to compound i administration . for treatment groups receiving compound i , carboplatin and pemetrexed , pemetrexed is administered 24 hours before compound i and carboplatin is administered 60 minutes before compound i . * effect of pemetrexed , carboplatin , and compound i relative to carboplatin + pemetrexed the data in tables 13 - 17 demonstrate that the potentiation of chemotherapeutic drug - induced tumor growth delay by compound i is statistically significant relative to the tumor growth delay caused by the chemotherapeutic drugs alone . add potassium carbonate ( 16 . 5 g , 120 mmol ) to a solution of dimethoxy acetaldehyde ( 60 % wt . in water ) ( 15 ml , 100 mmol ) and triethyl phosphonoacetate ( 20 ml , 100 mmol ) in 210 ml tetrahydrofuran and 30 ml water . stir the mixture at room temperature for 4 hours . pour the reaction mixture into diethyl ether ( 200 ml ) and wash with saturated aqueous sodium chloride . dry the organic phase over sodium sulfate and concentrate under reduced pressure to provide the desired compound as a yellow oil ( 15 . 8 g , 90 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 6 . 77 ( dd , j = 15 . 9 , 4 . 0 hz , 1h ), 6 . 13 ( dd , j = 15 . 9 , 1 . 4 hz , 1h ), 4 . 95 ( dd , j = 4 . 0 , 1 . 4 hz , 1h ), 4 . 22 ( q , j = 7 . 1 hz , 2h ), 3 . 34 ( s , 6h ), 1 . 30 ( t , j = 7 . 1 hz , 3h ). heat a mixture of 4 , 4 - dimethoxy - but - 2 - enoic acid ethyl ester ( 43 . 5 g , 250 mmol ) and p - toluenesulfonic acid ( 4 . 75 g , 25 mmol ) in acetonitrile ( 240 ml ) and water ( 15 ml ) at reflux for 2 hours . cool the reaction mixture to room temperature and add 2 - aminopyridine ( 18 . 8 g , 200 mmol ). heat the mixture at reflux for 16 hours then cool to room temperature . dilute the reaction mixture with ethyl acetate ( 1200 ml ) and wash sequentially with saturated aqueous sodium bicarbonate ( 600 ml × 3 ) and saturated aqueous sodium chloride ( 600 ml × 2 ). dry the organic phase over sodium sulfate and concentrate under reduced pressure to provide the desired compound as a brown oil ( 30 g , 73 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 8 . 06 ( d , j = 6 . 6 hz , 1h ), 7 . 63 ( d , j = 9 . 1 hz , 1h ), 7 . 56 ( s , 1h ), 7 . 20 ( dd , j = 8 . 9 , 6 . 8 hz , 1h ), 6 . 84 ( t , j = 6 . 7 hz , 1h ), 4 . 17 ( q , j = 7 . 3 hz , 2h ), 3 . 93 ( s , 2h ), 1 . 25 ( t , j = 7 . 3 hz , 3h ). heat a solution of imidazo [ 1 , 2 - α ] pyridin - 3 - yl - acetic acid ethyl ester ( 30 g , 147 mmol ) in nh 3 / meoh ( 7 n solution , 250 ml ) at 85 ° c . in a sealed tube for 15 hours . cool the reaction mixture to room temperature and concentrate under reduced pressure . treat the residue with dichloromethane , sonicate , and filter the resulting precipitate to provide the desired compound as a yellow solid ( 8 . 9 g , 35 %). 1 h - nmr ( 300 mhz , dmso ): δ 8 . 30 ( d , j = 6 . 9 hz , 1h ), 7 . 62 ( br s , 1h ), 7 . 54 ( d , j = 9 . 0 hz , 1h ), 7 . 42 ( s , 1h ), 7 . 21 ( dd , j = 7 . 7 , 6 . 7 hz , 1h ), 7 . 18 ( br s , 1h ), 6 . 91 ( t , j = 6 . 8 hz , 1h ), 3 . 81 ( s , 2h ). heat a solution of 5 - fluoro - 2 - nitro - benzaldehyde ( 10 g , 59 . 17 mmol ), butanol ( 20 ml , 219 mmol ) and p - toluenesulfonic acid ( 600 mg , 3 . 15 mmol ) in toluene ( 200 ml ) at reflux for 2 hours in a flask equipped with a dean - stark trap . cool the reaction mixture to room temperature , dilute with ethyl acetate ( 400 ml ), and wash sequentially with saturated aqueous sodium bicarbonate ( 300 ml × 3 ) and saturated aqueous sodium chloride ( 300 ml × 2 ). dry the organic phase over sodium sulfate and concentrate under reduced pressure to provide the desired compound as a pale yellow oil ( 17 g , 96 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 7 . 91 ( dd , j = 8 . 9 , 4 . 9 hz , 1h ), 7 . 53 ( dd , j = 9 . 3 , 2 . 9 hz , 1h ), 7 . 15 - 7 . 09 ( m , 1h ), 6 . 04 ( s , 1h ), 3 . 67 - 3 . 50 ( m , 4h ), 1 . 63 - 1 . 54 ( m , 4h ), 1 . 44 - 1 . 32 ( m , 4h ), 0 . 92 ( t , j = 7 . 3 hz , 6h ). add vinylmagnesium bromide ( 1 m in tetrahydrofuran , 85 . 2 ml , 85 . 2 mmol ) dropwise to a solution of 2 - dibutoxymethyl - 4 - fluoro - 1 - nitro - benzene ( 8 . 5 g , 28 . 4 mmol ) in tetrahydrofuran ( 250 ml ) at − 78 ° c . warm the reaction mixture − 45 ° c . to − 50 ° c . for 30 minutes , cool to − 78 ° c ., and add vinylmagnesium bromide ( 1 m in tetrahydrofuran , 85 . 2 ml , 85 . 2 mmol ) dropwise . warm the reaction mixture to − 45 ° c . to − 50 ° c . for 20 minutes , then add saturated aqueous ammonium chloride ( 300 ml ). warm the mixture to room temperature and extract with diethyl ether ( 200 ml × 2 ). wash the combined organic phases with saturated aqueous sodium chloride ( 400 ml × 2 ), dry over sodium sulfate , and concentrate under reduced pressure . dissolve the residue in tetrahydrofuran ( 100 ml ), add 0 . 5 n hcl ( 10 ml ), and stir for 20 minutes . dilute the mixture with diethyl ether ( 200 ml ), wash sequentially with saturated aqueous sodium bicarbonate ( 200 ml × 3 ) and saturated aqueous sodium chloride ( 200 ml × 2 ). dry the organic phase over sodium sulfate and concentrate under reduced pressure . subject the residue to silica gel chromatography , eluting with 5 % to 10 % ethyl acetate in hexanes to provide the desired compound as a pale yellow solid ( 2 . 6 g , 56 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 10 . 07 ( s , 1h ), 10 . 05 ( br s , 1h ), 7 . 62 ( d , j = 7 . 6 hz , 1h ), 7 . 42 - 7 . 39 ( m , 2h ), 6 . 60 ( d , j = 5 . 4 hz , 1h ). add 2 - aminoethanol ( 1 . 93 ml , 32 . 0 mmol ) followed by acetic acid ( 2 . 01 ml , 48 . 0 mmol ) to a solution of 5 - fluoro - 1h - indole - 7 - carbaldehyde ( 2 . 6 g , 16 . 0 mmol ) in 1 , 2 - dichloroethane ( 40 ml ). stir at room temperature for 15 minutes . add sodium triacetoxyborohydride ( 4 . 07 g , 19 . 2 mmol ) portionwise . stir the reaction mixture at room temperature for 3 hours . add saturated aqueous sodium bicarbonate ( 100 ml ) slowly followed by 1 n naoh to ph ˜ 9 . extract with ethyl acetate ( 100 ml × 3 ). wash the organic phase with saturated aqueous sodium chloride ( 200 ml × 2 ), dry over sodium sulfate , and concentrate under reduced pressure to provide the desired compound as a pale yellow solid ( 3 . 2 g , 96 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 9 . 71 ( br s , 1h ), 7 . 24 ( d , j = 2 . 7 hz , 1h ), 7 . 19 ( dd , j = 9 . 5 , 2 . 3 hz , 1h ), 6 . 79 ( dd , j = 9 . 8 , 2 . 2 hz , 1h ), 6 . 49 ( dd , j = 3 . 1 , 2 . 2 hz , 1h ), 4 . 15 ( s , 2h ), 3 . 77 ( t , j = 5 . 2 hz , 2h ), 2 . 84 ( t , j = 5 . 2 hz , 2h ). add a solution of di - tert - butyl dicarbonate ( 3 . 63 g , 16 . 65 mmol ) in tetrahydrofuran ( 40 ml ) dropwise to a solution of 2 -[( 5 - fluoro - 1h - indol - 7 - ylmethyl )- amino ]- ethanol ( 3 . 15 g , 15 . 14 mmol ) in tetrahydrofuran ( 60 ml ) at 0 ° c . stir the reaction mixture at room temperature for 2 hours . add ethyl acetate ( 200 ml ) and wash with saturated aqueous sodium chloride . dry the organic phase over sodium sulfate and concentrate under reduced pressure to provide the desired compound as a pale yellow oil ( 4 . 9 g , & gt ; 100 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 10 . 17 ( br s , 1h ), 7 . 27 - 7 . 23 ( m , 2h ), 6 . 81 ( dd , j = 9 . 4 , 2 . 4 hz , 1h ), 6 . 50 ( dd , j = 2 . 9 , 2 . 2 hz , 1h ), 4 . 67 ( s , 2h ), 3 . 72 ( br s , 2h ), 3 . 33 ( t , j = 5 . 3 hz , 2h ), 1 . 50 ( s , 9h ). add triethylamine ( 4 . 64 ml , 33 . 3 mmol ) followed by methanesulfonyl chloride ( 1 . 29 ml , 16 . 65 mmol ) to a solution of ( 5 - fluoro - 1h - indol - 7 - ylmethyl )-( 2 - hydroxyethyl )- carbamic acid tert - butyl ester ( 4 . 9 g , assume 15 . 14 mmol ) in dichloromethane ( 70 ml ) at 0 ° c . stir the reaction mixture for 30 minutes at 0 ° c . dilute with ethyl acetate ( 200 ml ), wash with sequentially with saturated aqueous sodium bicarbonate ( 200 ml × 3 ) and saturated aqueous sodium chloride ( 200 ml × 2 ). dry the organic phase over sodium sulfate and concentrate under reduced pressure to provide the desired compound as a yellow brown oil ( 5 . 9 g , & gt ; 100 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 10 . 07 ( br s , 1h ), 7 . 28 - 7 . 2 ( m , 2h ), 6 . 83 ( dd , j = 9 . 3 , 2 . 3 hz , 1h ), 6 . 50 ( dd , j = 2 . 9 , 2 . 2 hz , 1h ), 4 . 67 ( s , 2h ), 4 . 17 ( t , j = 5 . 5 hz , 2h ), 3 . 51 ( t , j = 5 . 6 hz , 2h ), 2 . 79 ( s , 3h ), 1 . 51 ( s , 9h ). add sodium hydride ( 60 %) ( 666 mg , 16 . 65 mmol ) in one portion to a solution of methanesulfonic acid 2 -[ tert - butoxycarbonyl -( 5 - fluoro - 1h - indol - 7 - ylmethyl )- amino ]- ethyl ester ( 5 . 9 g , assume 15 . 14 mmol ) in dimethylformamide ( 40 ml ) at 0 ° c . stir the reaction mixture at 0 ° c . for 10 minutes and then at room temperature for 30 minutes . add water ( 200 ml ) slowly . filter and dry the resulting yellow precipitate to provide the desired compound ( 4 . 14 g , 94 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 7 . 15 ( d , j = 9 . 1 hz , 1h ), 7 . 07 ( s , 1h ), 6 . 78 ( dd , j = 14 . 7 , 8 . 8 hz , 1h ), 6 . 49 ( d , j = 3 . 1 hz , 1h ), 4 . 81 ( s , 1h ), 4 . 76 ( s , 1h ), 4 . 25 - 4 . 23 ( m , 2h ), 3 . 94 - 3 . 83 ( m , 2h ), 1 . 49 ( s , 9h ). add oxalyl chloride ( 1 . 62 ml , 18 . 56 mmol ) to a solution of 9 - fluoro - 3 , 4 - dihydro - 1h -[ 1 , 4 ] diazepino [ 6 , 7 , 1 - hi ] indole - 2 - carboxylic acid tert - butyl ester ( 4 . 14 g , 14 . 28 mmol ) in methyl tert - butyl ether ( 100 ml ) at − 5 ° c . warm the reaction mixture to room temperature over 1 . 5 hours and then cool to − 5 ° c . add methanol ( 11 . 6 ml , 286 mmol ) and stir at − 5 ° c . for 30 minutes . add saturated aqueous sodium bicarbonate ( 100 ml ) and extract with ethyl acetate ( 100 ml × 3 ). wash the combined organic phase sequentially with saturated aqueous sodium bicarbonate ( 200 ml × 3 ) and saturated aqueous sodium chloride ( 200 ml × 2 ). dry the organic phase over sodium sulfate and then concentrate under reduced pressure to provide the title compound as a yellow solid ( 5 . 13 g , 93 %). 1 h - nmr ( 300 mhz , cdcl 3 ): δ 8 . 38 ( s , 1h ), 8 . 04 ( d , j = 6 . 8 hz , 1h ), 6 . 89 ( dd , j = 19 . 7 , 8 . 6 hz , 1h ), 4 . 90 ( s , 1h ), 4 . 81 ( s , 1h ), 4 . 45 - 4 . 43 ( m , 2h ), 4 . 05 - 3 . 93 ( m , 2h ), 3 . 95 ( s , 3h ), 1 . 42 ( s , 9h ). add potassium tert - butoxide ( 4 . 58 g , 40 . 92 mmol ) in one portion to a solution of 9 - fluoro - 7 - methoxyoxalyl - 3 , 4 - dihydro - 1h -[ 1 , 4 ] diazepino [ 6 , 7 , 1 - hi ] indole - 2 - carboxylic acid tert - butyl ester ( 5 . 13 g , 13 . 64 mmol ) and 2 - imidazo [ 1 , 2 - a ] pyridin - 3 - yl - acetamide ( 2 . 39 g , 13 . 64 mmol ) in dimethylformamide ( 80 ml ). stir the reaction mixture at room temperature for three hours . add saturated aqueous ammonium chloride ( 200 ml ) and extract with ethyl acetate ( 200 ml × 3 ). wash the combined organic phases with saturated aqueous sodium chloride ( 200 ml × 3 ), dry over sodium sulfate , and concentrate under reduced pressure . dissolve the residue in dichloromethane ( 20 ml ) and add 4n hcl in dioxane ( 40 ml ) dropwise , then stir at room temperature for 4 hours . filter the resulting precipitate and wash with diethyl ether to provide the title compound as a red solid ( 4 . 4 g , 68 %). ms ( apci ): m / z = 402 [ c 22 h 16 fn 5 o 2 + h ] + . add piperidine - 1 - carbonyl chloride ( 0 . 5 ml , 4 . 0 mmol ) to a solution of 3 -( 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro -[ 1 , 4 ] diazepino [ 6 , 7 , 1 - hi ] indol - 7 - yl )- 4 - imidazo [ 1 , 2 - a ] pyridin - 3 - yl - pyrrole - 2 , 5 - dione ( 1 . 42 g , 3 . 0 mmol ) and triethylamine ( 2 . 09 ml , 15 . 0 mmol ) in methanol ( 80 ml ). stir at room temperature over night . add triethylamine ( 1 . 04 ml , 7 . 5 mmol ) and piperidine - 1 - carbonyl chloride ( 0 . 5 ml , 4 . 0 mmol ). stir at room temperature for 5 hours . add ethyl acetate ( 500 ml ) and wash sequentially with saturated aqueous sodium bicarbonate ( 300 ml × 3 ) and saturated aqueous sodium chloride ( 200 ml ). dry the organic phase over sodium sulfate and concentrate under reduced pressure . subject the residue to silica gel chromatography , eluting with 0 % to 3 % methanol in ethyl acetate to provide the title compound as a red solid ( 700 mg , 45 %). ms ( apci ): m / z = 513 [ c 28 h 25 fn 6 o 3 + h ] + . heat a slurry of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine ( 500 mg , 0 . 976 mmol ) in methanol ( 2 . 5 ml ) to 64 ° c . add a solution of methanesulfonic acid ( 64 μl , 0 . 976 mmol ) in methanol ( 1 . 0 ml ) over 5 minutes . stir the mixture at 64 ° c . for 15 minutes and then add isopropanol ( 5 . 0 ml ) over 30 minutes . allow the resulting slurry to cool to room temperature over 1 hour and then stir at room temperature for 4 hours . filter the slurry , wash with isopropanol , and dry under reduced pressure at 42 ° c . to provide the title compound as an orange solid ( 478 mg , 88 . 5 % ( adjusted for 9 . 9 % volatiles in starting material and 1 . 0 % volatiles in product )). heat a slurry of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine ( 2 . 0 g , 3 . 9 mmol ) in ethanol ( 30 ml ) to 70 ° c . add 5m hcl ( 0 . 73 ml ) all at once . stir the mixture at 70 ° c . for 10 minutes and then add 1n naoh ( 3 . 63 ml ) over 3 minutes . stir the mixture at 70 ° c . for 2 hours . allow the resulting slurry to cool to room temperature over 1 hour and then stir at room temperature for 3 . 5 hours . filter the slurry , wash with ethanol , and dry under reduced pressure at 42 ° c . to provide the title compound as an orange solid ( 1 . 84 g , 92 % ( adjusted for 7 . 5 % volatiles in starting material and 7 . 7 % volatiles in product )). powder x - ray principal peaks ( degrees 2 theta , intensity ): 8 . 989 °, 100 %; 9 . 787 °, 48 . 7 %; 12 . 846 °, 20 . 0 %; and 7 . 444 °, 17 . 5 %. heat a slurry of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine ethanolate ( 198 . 5 mg ) in water ( 10 ml ) to 80 ° c . for 2 . 75 hours . add 3 . 11 ml of 1n hcl . when the temperature has returned to 80 ° c ., rapidly add 3 . 11 ml of 1n naoh . allow the temperature to remain at 80 ° c . for approximately 15 minutes then allow the suspension to cool to room temperature . collect the solid using vacuum filtration through whatman # 1 paper and allow to dry loosely covered over night . powder x - ray principal peaks ( degrees 2 theta , intensity ): 12 . 089 °, 100 %; 10 . 485 °, 83 . 6 %; 13 . 227 °, 56 . 0 %; and 7 . 660 °, 8 . 0 %. heat a slurry of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine ethanolate ( 200 . 6 mg ) in water ( 25 ml ) to 75 ° c . for 0 . 5 hours . add 0 . 72 ml of 1n hcl and continue to heat for 0 . 75 hours . rapidly add 0 . 72 ml of 1n naoh . allow the suspension to cool to room temperature . collect the solid using vacuum filtration through whatman # 1 paper , rinse with 20 ml deionized water and allow to dry loosely covered for 2 days . powder x - ray principal peaks ( degrees 2 theta , intensity ): 6 . 878 °, 100 %; 5 . 732 °, 58 . 7 %; 11 . 550 °, 82 . 8 %; 18 . 426 °, 20 . 7 %; and 10 . 856 °, 44 . 2 %. heat a slurry of 7 -( 2 , 5 - dihydro - 4 - imidazo [ 1 , 2 - a ] pyridine - 3 - yl - 2 , 5 - dioxo - 1h - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -( 1 - piperidinyl - carbonyl )- pyrrolo [ 3 , 2 , 1 - jk ][ 1 , 4 ] benzodiazepine ethanolate ( 200 . 8 mg ) in water ( 25 ml ) to 75 ° c . for 0 . 67 hours . add 0 . 72 ml of 1n hcl and continue to heat for 1 . 75 hours . add 0 . 1n naoh in 1 ml increments every 5 minutes until 7 . 2 ml have been added . after the last addition , allow the suspension to remain at 75 ° c . for 0 . 67 hours and then allow the suspension to cool to room temperature . collect the solid using vacuum filtration through whatman # 1 paper , rinse with 20 ml deionized water and allow to dry loosely covered for 2 days . powder x - ray principal peaks ( degrees 2 theta , intensity ): 5 . 498 °, 100 %; 22 . 149 °, 100 %; 14 . 921 °, 32 . 9 %; 11 . 399 °, 36 . 7 %; and 11 . 019 °, 20 . 5 %. compound i is preferably formulated as a pharmaceutical composition prior to administration to a patient . useful formulations comprise compound i or a pharmaceutically acceptable salt or solvate thereof and sbe7 - β - cd . the compound sbe7 - β - cd is a sulfobutyl ether of β - cyclodextrin described in u . s . pat . no . 5 , 134 , 127 . it is sold under the trade name captisol ®. particular formulations are described in the following formulation examples . a useful pharmaceutical composition may be prepared by dissolving compound i or a pharmaceutically acceptable salt or solvate thereof ( 50 mg / ml ) in 2 - pyrrolidone ( soluphor ®- p ). this solution is then diluted with an aqueous solution of sbe7 - β - cd ( 30 % by volume ) and poloxamer 188 ( lutrol ®- f 68 ) ( 10 % by volume ). prepare a first solution by adding 30 . 0 g sbe7 - β - cd to 71 . 25 ml of water and stir or agitate until completely dissolved . add 10 . 0 g poloxamer 188 and continue stirring until completely dissolved . prepare a second solution by adding compound i ethanolate to 2 - pyrrolidone according to the following formula : ml 2 - pyrrolidone =( actual compound i ethanolate wt ( mg )/ 50 mg / ml )× 0 . 5 . add the first solution to the second solution . filter the resulting solution through a 0 . 2 μm supor ® ( hydrophilic polyethersulfone ) filter ( pall corporation ) into a dust free container . a further pharmaceutical composition embodiment is prepared by combining compound i or a pharmaceutically acceptable salt or solvate thereof in an equimolar amount of a pharmaceutically acceptable acid in water . this mixture is then combined with at least one molar equivalent of sbe7 - β - cd as an aqueous solution . preferred pharmaceutically acceptable acids include hcl , hbr , sulfuric acid and methanesulfonic acid . the use of hcl is especially preferred . prepare a first solution by adding 20 . 0 g sbe7 - β - cd to 80 . 00 ml of water and stir or agitate until completely dissolved . add this solution to compound i ethanolate according to the following formula : ml of first solution =( actual compound i ethanolate wt ( mg )/ 20 mg / ml )−( actual compound i ethanolate wt ( mg )/ 1200 mg / ml )−( actual compound i ethanolate wt ( mg )× 0 . 00195107 ml of 1n hcl / mg compound i ethanolate ). add 1n hcl according to the following calculation : ml of 1n hcl to add =( actual compound i ethanolate wt ( mg )× 0 . 00195107 ml of 1n hcl / mg compound i ethanolate ). stir or bath sonicate until all compound has dissolved . a preferred pharmaceutical composition embodiment is prepared by adding 1 molar equivalent of compound i or a pharmaceutically acceptable salt or a solvate thereof to an aqueous solution of at least 1 molar equivalent of sbe7 - β - cd at a ph below 5 . 5 ( initial solution ph ), optionally in the presence of a pharmaceutically acceptable buffer , and mixing until the compound i or a pharmaceutically acceptable salt or solvate thereof has dissolved . the ph is then adjusted to between 2 . 5 and 3 . 5 with a pharmaceutically acceptable base ( final solution ph ). this resulting solution formulation may be administered to a patient directly , or the solution may preferably be lyophilized to provide a solid formulation capable of reconstitution with water . the sbe7 - β - cd may be present in the range of 1 molar equivalent up to an amount required to administer no more than 13 . 4 gm of sbe7 - β - cd to a patient in a day . a preferred amount of sbe7 - β - cd is from 1 . 0 to 4 . 0 molar equivalents , more preferred is from 2 . 0 to 3 . 0 molar equivalents , and from 2 . 5 to 2 . 7 molar equivalents relative to compound i is especially preferred . although any initial solution ph below 5 . 5 is acceptable , an initial solution ph below 3 . 0 is preferred , an initial solution ph in the range of 1 . 0 to 2 . 0 is more preferred , and an initial solution ph of between 1 . 2 and 1 . 4 is most preferred . the target initial solution ph is achieved by the addition of any pharmaceutically acid capable of adjusting the ph of the solution to a ph less than 5 . 5 . the use of hydrochloric acid is preferred . the formulation may optionally contain a pharmaceutically acceptable buffer . pharmaceutically acceptable buffers are those compounds employed by one skilled in the pharmaceutical formulation arts to stabilize the ph of a final solution in a particular ph range . pharmaceutically acceptable buffers include phosphate buffers as well as citric acid , glycine , and tartaric acid or pharmaceutically acceptable salts thereof . pharmaceutically acceptable salts of these acids include the sodium and potassium salts . it is preferred that a pharmaceutically acceptable buffer is present in the formulation . tartaric acid is a preferred pharmaceutically acceptable buffer . it is important that the compound i dissolve completely before the ph is adjusted to the final solution ph . dissolution may be assisted by any mechanical mixing means or by adjusting the temperature of the solution if necessary or desired . stirring the solution at room temperature is preferred . the final solution ph is achieved by the addition of any pharmaceutically acceptable base capable of adjusting the ph of the solution to a ph in the range of 2 . 5 to 3 . 5 . the use of sodium hydroxide is preferred . the final solution ph may be in the range of 2 . 5 to 3 . 5 , but is preferably in the range of 2 . 5 to 3 . 1 . a final solution ph in the range of 2 . 7 to 3 . 1 is most preferred . once the final solution ph has been achieved , the solution may be lyophilized if necessary or desired under standard lyophilization conditions to provide a solid pharmaceutical composition suitable for reconstitution with water . prepare a solution of 0 . 15 g tartaric acid and 12 g ( 5 . 55 mmol ) sbe7 - β - cd in 70 ml of water . add 5 ml of 1 . 0 n hcl and mix at room temperature . add 1 . 1 g ( 2 . 15 mmol ) compound i ethanolate and stir at room temperature until dissolved . add 1n sodium hydroxide to a ph of about 2 . 9 . add sufficient water to achieve a final volume of 100 ml . lyophilize this solution to provide an amorphous orange - red solid .
a method for treating gastric cancer , ovarian cancer , non - small cell lung cancer , or colorectal cancer in a patient is described , as well as pharmaceutical compositions comprising 7 - pyridine - 3 - yl - 2 , 5 - dioxo - ih - pyrrol - 3 - yl )- 9 - fluoro - 1 , 2 , 3 , 4 - tetrahydro - 2 -- pyrrolobenzodiazepine , useful for the method and a process for preparing said compositions .