we have discovered and hereby disclose a method for effectively causing regression of established malignant tumors in rats , mice and hamsters requiring such treatment by administering heteropolysaccharide hexosaminoglycan sulfate ( hhs ) together with available steroids , their isomers or epimers . pharmaceutical compositions , oral and parenteral dosage forms suitable for carrying out the method of the present invention are also disclosed . hexuronyl hexosaminoglycan sulfate ( hhs ) is used in the present invention is a purified product of a known , naturally occurring polysaccharide of the type described in u . s . pat . no . 4 , 230 , 699 to fussi et al and u . s . pat . no . 4 , 264 , 733 also to fussi et al ; see also u . s . pat . no . 4 , 143 , 132 . the preferred hexuronyl hexosaminoglycan sulfate is in accordance with claim 1 of u . s . pat . no . 4 , 230 , 699 ; see also the discussion beginning at line 33 , column 2 of the same patent . hhs is described in these patents and elsewhere in the art as exhibiting anticoagulant , antithrombotic and clearing activities , suitable for either oral or parenteral administration . the discovery of these patents are hereby incorporated by reference . the hhs used in accordance with the present invention consists is further characterized as follows : pharmaceutical presentation -- the compositions according to the invention are conveniently in a form suitable for oral , rectal , parenteral or topical administration in a suitable pharmaceutical vehicle and in any case very well known generally for any particular kind of preparation . thus for example tablets , capsules , ingestible liquid or powder preparations , creams and lotions for topical application , or suppositories , can be prepared as required . injectable solutions are also within the ambit of the invention . it will be understood that the absolute quantity of active ingredients present in any dosage unit should not exceed that appropriate to the rate and manner of administration to be employed but on the other hand should also desirably be adequate to allow the desired rate of administration to be achieved by a small number of doses . the rate of administration will moreover depend on the precise pharmacological action desired . the quantity of hhs administered varies in accordance with a number of factors including the physical condition and tumor tissue involvement of the rat , mouse or hamster requiring treatment , the rat &# 39 ; s , mouse &# 39 ; s , or hamster &# 39 ; s body weight and the regimen of treatment that is adopted . the amount of hhs is anticipated to be within the range of 50 mg to 5 , 000 mg per day with amounts in the range of at least 100 to 200 mg / day up to 0 . 5 to 2 grams / day being typical . method of treatment and pharmaceutical compositions in accordance with the present invention are assessed using art - recognized animal model studies as reported below . in the following examples all parts and percentages are by weight and all temperatures reported in degrees centigrade unless otherwise stated . hhs has been tested to assess the pharmacological properties and its activity has been demonstrated . two of the models used are virally induced new murine mammary adenocarcinomas , whereby metastatic tumors are transplated to the mammary gland of mice or rats . selected animals received controlled amounts of heparin sodium , u . s . p . manufactured by hepar industries , inc ., franklin , ohio ( hepar ), or by abbott laboratories , inc . ( panheparin ) or hhs . ______________________________________ tumor volume in cmm after 25 days 30 days 35 days 40 days______________________________________control 100 450panheparin 250 300 350 100 ( 1000 u / ml ) hepar 100 105 120 100 ( 1000 u / ml ) hhs 45 25 ( 5 mg / ml ) ______________________________________ results indicate significant reduction in tumor volume using the hhs hydrocortisone combination treatment as compared with hepar and panheprin . ______________________________________ tumor volume in cmm after 12 days 15 days 20 days______________________________________no treatment 70 100 400control 75 100 210panheparin ( 1000 u / ml ) 75 90 200hhs ( 5 mg / ml ) 200 175______________________________________ this pancreatic cancer model in hamsters was selected to evaluate the effectiveness of hhs on a soft tissue tumor with the following results : ______________________________________ no . of tumor growth animals rate * p - value ** ______________________________________no treatment 5 . 44 -- heparin alone 5 . 39 insignificanthepar + low dose 5 . 33 . 01hydrocortisonehepar + high dose 4 . 33 . 04hydrocortisonehhs alone 5 . 24 . 01hhs + low dose 5 . 34 . 01hydrocortisonehhs + high dose 5 . 39 insignificanthydrocortisone______________________________________ * slope of regression line ** compared to no treatment interestingly , hhs alone significantly reduces tumor growth in hamsters possibly indicating that the steroids act as a catalyst or as an immune system repressant . on the contrary , hhs could also act as the endogenous stimulator of other inhibitors , although we do not wish to be bound to a particular theory of operation or activity . the efficacy of heparin ( hep ), the heparin analogue hexuronyl hexosaminoglycan sulfate ( hhs ), and hydrocortisone ( hc ) in inhibiting the growth of pancreatic cancer was studied by an investigaror at the national cancer institute , national institutes of health , bethesda , md ., usa . the model system chosen utilized 4 morphologically distinct pancreatic adenocarcinoma lines transplantable in syrian hamster strains : cbp , lhp , lsp - 3 , and pour - lvg . syngeneic hamsters were inoculated with ld 100 doses of 1 of the 4 tumor lines and were randomly allocated to groups of 5 animals which received continuously in their drinking water : hep ( 100 u / ml ), hhs ( 10 mg / ml ) alone , hc ( 0 . 5 mg / ml alone , hep + hc , hhs + hc , or no additives ( control . tumors were measured , growth rates calculated , and nonparametric statistical comparisons made among median growth rates of all groups . to confirm the capacity to produce angiogenesis factor , all tumors were tested in the rabbit cornea assay . in addition , tumors growing in treated animals were excised , and extracts were prepared for quantitative testing of in vitro angiogenesis in the boyden chamber bovine adrenal capillary endothelial cell migration assay . all tumors produced angiogenesis in the rabbit cornea . no inhibition of tumor growth in any of the 4 tumor systems were observed , when compared to untreated with hc alone , hep alone , hhs alone , or hep + hc . reduction in median tumor growth rate was observed in animals treated with hhs + hc bearing cbp ( p & lt ; 0 . 05 ), and lsp - 3 ( p = 0 . 06 ) tumors , i . e , 3 of the 4 tumor lines used . similarly , in vitro capillary cell migration was decreased by hhs + hc treatment in the cbp ( p & lt ; 0 . 005 ), pour - lvg ( p & lt ; 0 . 01 ), and lsp - 3 ( p & lt ; 0 . 05 ) models . lhp tumor showed no effect of hhs + hc treatment on tumor growth rate or on endothelial cell migration . from these animal model studies , it may be concluded that when hhs is administered orally with hc , tumor growth in rats , mice and hamsters is inhibited in 3 of 4 transplantable pancreatic carcinoma lines , possibly through mechanisms inhibiting tumor angiogenesis in rats , mice and hamsters .
regression of established malignant tumors in rats , mice and hamsters is achieved with a combination of the heparin fragment hexuronyl hexosaminoglycan sulfate together with a steroid such as hydrocortisone . hhs is clinically useful for its antineoplastic , inhibiting effects in tumor systems in rats , mice and hamsters .