the disclosure of u . s . pat . no . 4 , 822 , 349 , issued apr . 18 , 1989 , is incorporated by reference herein . a number of blood coagulation accelerator materials were mixed with fresh blood samples using the blood of different animals from several animal species , including horse , cow and dog . the materials and blood were mixed in predetermined ratios and the time at which the blood in the test mixture clotted was recorded . this type of clotting test is called “ the whole - blood coagulation test ” and is widely performed , such as in monitoring heparin therapy . this type of test is neither the most sensitive or most precise clotting test known . however , in the present application this test allowed rapid and inexpensive screening of materials that exhibited increased blood coagulation effects from those materials that exhibited little or no blood coagulation effects . while animals were used as test subjects it is believed human blood will react in substantially the same fashion . therefore the invention is applicable to both veterinary and human use . without wishing to be bound to any theory , it is thought that some blood coagulation accelerator materials selectively absorb small molecules such as water in blood . the absorption of small molecules increases the rate of blood clotting . further , the dehydration reactions generally evolve heat , which is helpful in arresting wound bleeding . preferably , the molecular sieve and inorganic oxide materials incorporate ca ions . the incorporation of ca ions into molecular sieve and inorganic oxide materials is shown to increase the effectiveness of such materials in arresting wound bleeding . it should be noted that the results of this test procedure vary somewhat with different observers for the same blood coagulation accelerator materials . additionally , some blood coagulation accelerator materials such as mgo form a slurry when mixed with blood , making determination of the clotting time difficult . some of the blood coagulation accelerator materials that lowered blood - clotting time were mixed , and these mixtures were tested for effect on blood clotting . additionally , some blood coagulation accelerator materials were coated on various substrates or contained within packages . blood coagulation accelerator materials were also used to control bleeding in animals . the blood coagulation accelerator materials were either obtained commercially or prepared in the laboratory . materials commercially available are avitene , a microfibrillar collagen hemostat available from davol inc . of cranston r . i . ; ( na ) zeolite 4a , (( na ) 12 [( alo 2 ) 12 ( sio 2 ) 12 ]. 27h 2 o ), available under the trade name purmol 4a from zeochem of louisville , k . y . ; ( ca ) zeolite 5a (( ca ) 6 [( alo 2 ) 12 ( sio 2 ) 12 ]. 27h 2 o ), available under the trade name linde type a from union carbide ; yunnan white medicine available from china ; maxwell house coffee available from kraft ; sodium aluminum oxide ( naalo 2 ), available from alfa aesar company of ward hill , mass . ; magnesium oxide ( mgo ), calcium oxide ( cao ), phosphorus pentoxide ( p 2 o 5 ), acid silica ( sio 2 . nh 2 o ), chromatographic silica gel ( sio 2 ), calcium chloride ( cacl 2 ), secondary dibasic calcium phosphate ( cahpo 4 ), titanium dioxide ( tio 2 ), available as titanic oxide , and barium oxide ( bao ), all available from fisher scientific company ; activated carbon , available from strem chemicals of newburyport mass . ; europium oxide ( eu 2 o 3 ) and cerium oxide ( ceo 2 ), available from american potash & amp ; chemical corp . of west chicago , ill . ; copper oxide ( cuo ), available from cerac inc . of milwaukee , wis . ; cobalt oxide ( co 2 o 3 ), available from j . t . baker ; bismuth oxide ( bi 2 o 3 ), available from baker and adamson chemical co . ; aluminum oxide ( al 2 o 3 ), available as aluminum oxide neutral type t from em reagents ; nickel oxide ( nio ), available from matheson , coleman and bell of east rutherford , n . j . ; zinc oxide ( zno ), stannic oxide ( sno 2 ), and iron oxide , ( fe 2 o 3 ) all available from baker analyticals ; manganese oxide ( mno ), available as manganese iv oxide , 99 % and zirconium ( iv ) oxide ( zro 2 ), all available from aldrich ; vanadium pentoxide ( v 2 o 5 ), available from mallinkrodt ; scandium oxide ( sc 2 o 3 ), available as scandium oxide 98 % from a . d . mackay of new york ; yttrium oxide ( y 2 o 3 ), available from alfa aesar company of ward hill , mass . ; chromosorb p - aw - dmcs , chromosorb 101 , and chromosorb 102 , all available from alltech associates , deerfield ill . ; zsm - 5 available from amoco chemical and ca - montmorillonite , ( na , ca )( al , mg ) 6 ( si 4 o 10 ) 3 ( oh ) 6 . nh2o available under the tradename carbosorb sm 1502 from gsa resources of cortaro , ariz . avitene is a commercially available blood coagulation accelerator material which consists of collagen . avitene was used for comparative purposes . additional blood coagulation accelerator materials were prepared in the laboratory . materials synthesized in the laboratory include silica gel ( sio 2 ); alumina gel ( al 2 o 3 ); ( na ) zeolite 4a (( na ) 12 [( alo 2 ) 12 ( sio 2 ) 12 ]. 27h 2 o y (( na ) 56 ( al 56 si 136 o 384 ). 250h 2 o )); ( ca ) zeolite y (( ca , na ) 56 ( al 56 si 136 o 384 ). 250h 2 o )); ( k ) oms - 2 (( k ) mn 8 o 16 . nh 2 o ) ( ca ) oms - 2 (( ca , k ) mn 8 o 16 . nh2o ); ldh , mg x al y ( oh ) z cl u . nh 2 o ; chabazite ( k 11 ( al 11 si 25 o 72 ). 40h 2 o ); ( ca ) ol - 1 (( ca , k , na ) mn 14 o 27 . 21h 2 o ); zsm - 5 ( na 7 ( al 7 si 89 o 192 ). nh 2 o ); ( ca ) zsm - 5 (( ca , na ) 7 ( al 7 si 89 o 192 ). nh 2 o ) zeolite rho , ( na , cs ) 12 [ al 12 si 36 o 96 ]. 44h2o ; ca - mordenite , ( na - ca ) 5 [ al 5 si 43 o 96 ]. nh 2 o ; silica - alumina , sio 2 - al 2 o 3 ; and silica - calcia sio 2 - cao . these materials were prepared as described below . physical and chemical confirmations of the prepared materials were done using x - ray diffraction ( xrd ), scanning electron microscopy ( sem ) and energy dispersive x - ray ( edx ). 500 milliliters ( ml ) of 1 molar calcium ion solution was prepared by dissolving calcium acetate or calcium chloride in the requisite quantity of water . 10 grams ( gm ) of ( k ) oms - 2 was added to the calcium ion solution and the mixture was stirred for twenty - four hours . after stirring , the mixture was filtered and the solids were washed using distilled water . the washed solids were dried in an oven at 100 ° c . for 24 hours . ( ca ) zeolite y was prepared by substituting 10 gm of ( na ) zeolite y for oms - 2 in the ( ca ) oms - 2 procedure . solution a ( 100 ml of water glass ( sio2 / na 2 o = 3 . 22 )+ 200 ml of deionized distilled water ( ddw )) and solution b ( 49 ml of 4 . 0 n hcl + 60 ml of h 2 o ) were both cooled to about 5 ° c . solution a was added to solution b with vigorous agitation . the resultant solution was poured into a flat tray to gel . after about 30 minutes the resulting stiff gel was cut into cubes . the cubes were transferred to a buchner funnel and treated immediately with 1 n hcl for two hours . the hcl treatment procedure was twice more repeated . the gel was then washed free of chloride ions and subsequently dried for eight hours at 150 ° c . in an electric oven . solution a was prepared by dissolving 57 gram of alcl 3 . 6h 2 o in 1000 ml ddw . solution b was prepared by diluting 84 ml of concentrated ammonium hydroxide to 145 ml ddw . solution b was added to solution a with stirring . the precipitate was settled , filtered through a buchner funnel and washed five times with very dilute ammonia solution ( 1 ml of concentrated ammonia + 1000 ml ddw ). then the precipitate was dried for eight hours at 120 ° c . in an electric oven . zeolite 4a was prepared according to the procedure described in microiorous and mesoporous materials , 22 : 551 - 666 , robson , h . ( editor ), elsevier , amsterdam ( 1998 ); the disclosure of which is incorporated by reference herein . zeolite y was prepared according to the procedure described in microporous and mesororous materials , 22 : 551 - 666 ( 1998 ). 11 grams ( gm ) of mnac 2 . 6h2o in 40 ml ddw was dissolved in a buffer solution consisting of 5 ml of acetic acid and 5 gram of kac in 40 ml of ddw . a solution of 6 . 5 gram of kmno 4 in 150 ml was then added slowly with a burette . the resultant gel was refluxed with stirring for 24 hours . the product was filtered through a buchner funnel , washed 3 - 5 times using 500 ml aliquots of ddw and dried for 24 hours at 110 ° c . in an electric oven . layered double hydroxide material was prepared according to the procedure described in miyata , s . clays and clay materials , 23 : 369 - 375 ( 1975 ); the disclosure of which is incorporated by reference herein . chabazite was prepared according to the procedure described in microporous and mesoporous materials , 22 : 551 - 666 ( 1998 ). 19 gm of mncl2 . 4h2o and 6 gm of mgcl2 . 6h2o in 120 ml ddw were dissolved in a solution consisting of 30 gm naoh and 150 ml ddw . a solution of 3 . 8 gm kmno 4 in 140 ml ddw was added slowly with a burette . the product was kept for overnight at 60 ° c . in an electric oven . the precipitate was filtered through a buchner funnel , washed 3 - 5 times using 500 ml ddw and dried for 24 hours at 110 ° c . in an electric oven . calcium type ol - 1 was prepared by substituting 10 gm of ol - 1 for oms - 2 in the ( ca ) oms - 2 procedure . calcium type zsm - 5 was prepared by substituting zsm - 5 for oms - 2 in the ( ca ) oms - 2 procedure . 4 m na 2 alo 2 oh was prepared as follows : 294 . 3 gm of catapal sb ( available from condea of louisiana ) and 320 gm of naoh pellets were added to 800 ml h 2 o and placed in oven at 100 ° c . for five days . after heating , the solution was cooled and diluted to 1 l . solution a comprising 200 ml of 4 m na 2 alo 2 oh , 32 gm of naoh pellets and 56 ml of 50 % csoh solution was prepared . 720 ml of ludox ls - 30 ( available from dupont ) was added to solution a . the mixture was shaken until it was homogeneous , and allowed to stand at room temperature for five days . the resultant solution was heated in an electric oven at 90 ° c . and shaken daily for 1 - 3 weeks . mordrdenite was prepared according to the procedure described in microporous and mesodorous materials , 22 : 551 - 666 ( 1998 ). calcium type mordenite was prepared by substituting 10 gm of mordenite for oms - 2 in the ( ca ) oms - 2 procedure . solution a was prepared by dissolving 233 ml of water glass in 450 ml of ddw . solution b was prepared by dissolving 34 ml 4 n hcl and 23 gm of al 2 ( so 4 ) 3 . 18h 2 o in 200 ml ddw . both solution a and solution b were cooled to about 5 ° c . solution a was added to solution b rapidly with strong agitation . the resultant mixture was poured into a flat tray to gel and cut into cubes after one hour . the gel cubes were aged for 48 hours and transferred to a buchner funnel . a 2 % solution of al 2 ( so 4 ) 3 . 18h 2 o was used to do the base exchange three times for 2 - hour periods , then once overnight . the product was washed using ddw until free of sulfate ions and dried for eight hours at 170 ° c . in an electric oven . silica - calcia was prepared according to the procedure described in banal , n . p ., j . am . ceram . soc . 71 ( 8 ): 666 - 672 ( 1998 ); the disclosure of which is incorporated by reference herein . it should be understood that the following examples are included for purposes of illustration so that the invention may be more readily understood and are in no way intended to limit the scope of the invention unless otherwise specifically indicated . the blood coagulation testing was performed using the following procedure . silicon oxide treated sterile 7 ml vacutainer tubes were pre - weighed . a predetermined amount of each blood coagulation accelerator material was transferred into the pre - weighed tube . the pre - weighed tube , with material inside , was weighed and dried in an oven at 100 ° c . for at least 24 hours . each tube was sealed with a septum in an inert gas atmosphere . the prepared tubes were allowed to cool , and weighed before and after use in the blood coagulation test . 20 ml syringes with needles were used to collect 10 ml of fresh blood from the jugular vein of a subject animal . 1 ml of the fresh blood was quickly dispensed to each pre - weighed tube containing a predetermined amount of blood coagulation accelerator material to give the ratio of material to blood indicated in the tables . the tubes were shaken to mix the blood and material together . testing was done in batches of 6 test tubes at one time . timing was started when the tube was initially filled with blood . after filling and mixing , each tube was monitored visually until a blood clot was seen . the coagulation time was recorded when the blood formed a solid clot with no flowing movement of the mixture observable within the tube . the time required for blood clotting in a glass tube is a measure of the overall activity of the intrinsic system in blood coagulation . periodic inspection of the clot permits evaluation of its physical properties ( appearance , size and mechanical strength ), its stability and the rate and extent of its retraction . see hematology , william j . williams , editor , 1661 mcgraw - hill ( 3rd edition , 1983 ), the entire disclosure of which is incorporated by reference herein . temperature changes of the tubes during testing were detected by a thermocouple attached to the external wall of the tube . initial and maximum temperatures were recorded . the reaction between the blood sample and phosphorous pentoxide was severe enough to raise the blood sample to the boiling point . with the above exception , reactions between blood coagulation accelerator materials and the blood samples were within the range of − 2 ° c . to 8 ° c . the temperature changes for some materials are summarized in table 1 . blank tubes that did not contain a blood coagulation accelerator material were used to establish a base line coagulation time for each sampling date and blood donor . this was done to minimize the influence of temperature , atmospheric and other environmental variables . since the coagulation time of the blank tubes varied , a relative coagulation time was used to compare the effect of each blood coagulation accelerator material on blood clotting times . relative coagulation time was calculated using the following equation . relative coagulation time =(( coagulation time of blood exposed to blood coagulation accelerator material )/( coagulation time of blood in blank tube ))× 100 . relative coagulation time is more precise than absolute coagulation time because environmental errors are lessened through the use of blank tests . the results of the individual blood coagulation tests are shown in tables 2 through 14 . table 16 is a compilation of relative coagulation times for all materials . as can be seen from table 16 , many of the materials provide surprising and unexpected decreases in blood coagulation time . given the present invention , other clays , zeolite materials , oxides and combinations thereof would also be expected to show similar advantageous effects in animal and human systems and their use is fully comprehended by the invention . blood coagulation testing was also performed on mixtures of blood coagulation accelerator materials . the mixtures were prepared by mixing sodium aluminum oxide with another blood coagulation accelerator material that had proven individually to be effective in initiating and accelerating blood clotting . each combination material contained approximately 50 % sodium aluminum oxide and 50 % blood coagulation accelerator material by weight . the mixtures of materials were prepared and tested using the above - described procedure for single blood coagulation accelerator materials . the results of the blood coagulation testing for mixed materials are shown in table 15 . as can be seen from table 15 , many of the mixed materials provide surprising and unexpected decreases in blood coagulation time when compared to the times for materials used alone . certain calcium containing materials appear to be beneficial in promoting blood clotting . as shown in table 16 , powdered calcium oxide was found to speed blood clotting significantly . while not wishing to be held to a particular theory , the inventors believe that calcium ions can be essential for interaction with calcium ion dependent enzymes and blood clotting factors during homeostasis . the inventors also believe that calcium ions are important for platelet activation , activation of phospholipases , activation of calcium dependent proteases and other functions . zeolite materials containing calcium cations were also tested for blood coagulation times . as can be seen from table 12 , the calcium exchanged versions of zeolite y and oms - 2 were surprisingly more effective than the non - calcium exchanged version in accelerating blood coagulation . the capacity of zeolite materials to exchange ions is dependent on the size of the pores or channels therein , size of the ions , temperature and other factors . materials such as ( na ) zeolite y and ( na ) zeolite a can exchange the sodium ion inside their channels with calcium in the blood , removing calcium ions from the blood and retarding the coagulation process . with calcium exchanged zeolite materials there is no possibility of such removal of calcium ions from the blood , therefore blood coagulation is not retarded by the ion exchange . not all calcium exchanged zeolite materials exhibit this beneficial behavior . as can be seen from tables 13 and 14 , ( ca ) zsm - 5 , while advantageous in accelerating coagulation time , was somewhat less effective than non - calcium exchanged zsm - 5 . [ 0062 ] table 2 test 1 , day 1 , horse blood from horse 2 average relative test average test coagulation coagulation coagulation material / blood material / blood material sample # time time time % ratio gm / ml gm / ml blank 1 00 : 25 : 38 00 : 30 : 15 100 2 00 : 34 : 52 oms - 2 ( ca ) + naalo 2 1 00 : 10 : 30 1 00 : 00 : 35 1 . 9 0 . 2941 0 . 3320 2 00 : 00 : 35 0 . 3698 mgo + naalo 2 1 00 : 01 : 02 00 : 01 : 01 3 . 4 0 . 3967 0 . 3822 2 00 : 01 : 00 0 . 3677 zeolite y ( ca ) + naalo 2 1 00 : 01 : 41 00 : 01 : 09 3 . 8 0 . 3535 0 . 3660 2 00 : 00 : 37 0 . 3785 cerium oxide + naalo 2 1 00 : 00 : 46 00 : 01 : 15 4 . 1 0 . 4308 0 . 4023 2 00 : 01 : 44 0 . 3739 cao + naalo 2 1 00 : 01 : 35 00 : 01 : 18 4 . 3 0 . 3075 0 . 3129 2 00 : 01 : 01 0 . 3182 silica gel + naalo 2 1 00 : 01 : 41 00 : 01 : 28 4 . 8 0 . 3094 0 . 3324 2 00 : 01 : 15 0 . 3553 [ 0063 ] table 3 test 2 , day 1 , cow blood from cow 1 average relative test average test coagulation coagulation coagulation material / blood material / blood material sample # time time time % ratio gm / ml gm / ml blank 1 00 : 13 : 24 00 : 13 : 23 100 2 00 : 13 : 21 naalo 2 1 00 : 00 : 27 00 : 00 : 25 3 . 1 0 . 2544 0 . 3039 2 00 : 00 : 22 0 . 3534 oms - 2 1 00 : 00 : 33 00 : 00 : 32 4 . 0 0 . 4439 0 . 4031 ( ca ) 2 00 : 00 : 30 0 . 3622 mgo 1 00 : 00 : 39 00 : 00 : 38 4 . 7 0 . 3387 0 . 3270 2 00 : 00 : 36 0 . 3152 cao 1 00 : 00 : 39 00 : 00 : 38 4 . 7 0 . 3777 0 . 3702 2 00 : 00 : 36 0 . 3627 silica gel 1 00 : 00 : 49 00 : 00 : 47 5 . 9 0 . 3135 0 . 3034 2 00 : 00 : 44 0 . 2933 cerium oxide 1 00 : 03 : 14 00 : 03 : 13 24 0 . 4661 0 . 4093 2 00 : 03 : 11 0 . 3524 zeolite 5a 1 00 : 04 : 09 00 : 04 : 09 31 0 . 3391 0 . 3264 2 00 : 04 : 08 0 . 3137 zeolite y 1 00 : 07 : 49 00 : 07 : 33 56 0 . 3849 0 . 3864 ( ca ) 2 00 : 07 : 16 0 . 3878 mgo + naalo 2 1 00 : 00 : 16 00 : 00 : 14 1 . 7 0 . 4663 0 . 4127 2 00 : 00 : 11 0 . 3591 silica gel + naalo 2 1 00 : 00 : 30 00 : 00 : 27 3 . 4 0 . 3163 0 . 3450 2 00 : 00 : 24 0 . 3744 zeolite y ( ca ) + naalo 2 1 00 : 00 : 31 00 : 00 : 28 3 . 5 0 . 3998 0 . 3747 2 00 : 00 : 25 0 . 3496 cao + naalo 2 1 00 : 00 : 35 00 : 00 : 34 4 . 2 0 . 3381 0 . 3758 2 00 : 00 : 32 0 . 4136 oms - 2 ( ca ) + naalo 2 1 00 : 00 : 41 00 : 00 : 40 4 . 9 0 . 2926 0 . 3552 2 00 : 00 : 38 0 . 4178 cerium oxide + naalo 2 1 00 : 04 : 43 00 : 04 : 56 37 0 . 3447 0 . 3310 2 00 : 05 : 09 0 . 3173 [ 0064 ] table 4 test 3 , day 1 , dog blood from dog 1 average relative test average test coagulation coagulation coagulation material / blood material / blood material sample # time time time % ratio gm / ml gm / ml blank 1 00 : 07 : 49 00 : 08 : 02 100 2 00 : 08 : 14 naalo 2 1 00 : 00 : 34 00 : 00 : 33 6 . 9 0 . 2466 0 . 3425 2 00 : 00 : 31 0 . 4384 oms - 2 1 00 : 00 : 35 00 : 00 : 34 7 . 0 0 . 3422 0 . 3619 ( ca ) 2 00 : 00 : 32 0 . 3816 silica gel 1 00 : 00 : 41 00 : 00 : 39 8 . 1 0 . 3839 0 . 3681 2 00 : 00 : 37 0 . 3941 cerium oxide 1 00 : 02 : 13 00 : 02 : 10 27 0 . 3780 0 . 3706 2 00 : 02 : 07 0 . 3633 [ 0065 ] table 5 test 4 , day 2 , dog blood from dog 1 average relative average test coagulation coagulation coagulation average ratio material / blood material sample # time time time % material / blood gm / ml blank 1 0 : 04 : 36 0 : 05 : 05 100 2 0 : 05 : 34 ceo + naalo 2 1 0 : 00 : 04 0 : 00 : 04 1 . 31 0 . 3382 0 . 3371 2 0 : 00 : 04 0 . 3394 cao + naalo 2 1 0 : 00 : 38 0 : 00 : 37 12 . 13 0 . 3873 0 . 4082 2 0 : 00 : 36 0 . 3664 cao 1 0 : 00 : 59 0 : 00 : 43 14 . 10 0 . 3858 0 . 3829 2 0 : 00 : 27 0 . 3886 naalo 2 1 0 : 00 : 43 0 : 01 : 00 19 . 67 0 . 3636 0 . 3725 2 0 : 01 : 17 0 . 3546 oms - 2 ( ca ) + naalo 2 1 0 : 01 : 21 0 : 01 : 02 20 . 16 0 . 4244 0 . 383 2 0 : 01 : 19 0 . 4605 zeolite y 1 0 : 01 : 21 0 : 01 : 20 26 . 23 0 . 3847 0 . 3951 ( ca ) + 2 0 : 01 : 19 0 . 3743 naalo 2 silica 1 0 : 01 : 30 0 : 01 : 29 29 . 02 0 . 3332 0 . 3636 gel + naalo 2 2 0 : 01 : 27 0 . 3028 mgo + 1 0 : 01 : 42 0 : 01 : 39 32 . 46 0 : 3215 0 . 2964 naalo 2 2 0 : 01 : 36 0 . 3466 zeolite y 1 0 : 01 : 53 0 : 01 : 51 36 . 23 0 . 3235 0 . 3190 ( ca ) 2 0 : 01 : 48 0 . 3279 zeolite 5a 1 0 : 02 : 03 0 : 02 : 01 39 . 67 0 : 3301 0 . 3303 2 0 : 01 : 59 0 . 3299 mgo 1 0 : 01 : 59 0 : 05 : 34 109 . 67 0 . 3465 0 . 3488 2 0 : 09 : 10 0 . 3442 [ 0066 ] table 6 test 5 , day 3 , horse blood from horse 1 average relative average test coagulation coagulation coagulation average ratio material / blood material sample # time time time % material / blood gm / ml blank 1 0 : 13 : 43 0 : 14 : 47 100 2 0 : 14 : 16 2 0 : 16 : 23 naalo 2 1 0 : 01 : 06 0 : 00 : 53 6 . 01 3 . 3882 2 0 : 00 : 39 0 . 3853 0 . 3864 3 0 : 00 : 55 0 . 3813 cao 1 0 : 01 : 45 0 : 01 : 21 9 . 09 0 . 3909 2 0 : 01 : 50 0 . 3880 0 . 3609 3 0 : 00 : 27 0 . 4121 cerium 1 0 : 01 : 41 0 : 01 : 16 8 . 56 0 . 3756 0 . 3251 oxide / naalo 2 2 0 : 00 : 34 0 . 3792 3 0 : 01 : 33 0 . 4226 [ 0067 ] table 7 test 6 , day 3 , horse blood from horse 2 average relative average test coagulation coagulation coagulation average ratio material / blood material sample # time time time % material / blood gm / ml blank 1 0 : 12 : 56 0 : 15 : 51 100 2 0 : 16 : 02 2 0 : 18 : 36 naalo 2 1 0 : 00 : 30 0 : 00 : 42 4 . 38 0 . 4160 0 . 4059 2 0 : 00 : 43 0 . 3989 3 0 : 00 : 52 0 . 4433 cao 1 0 : 04 : 31 0 : 03 : 42 23 . 34 0 . 4035 0 . 3994 2 0 : 02 : 16 0 . 4247 3 0 : 04 : 19 0 . 3864 [ 0068 ] table 8 test 7 , day 3 , horse blood from horse 3 relative average average ratio average test coagulation coagulation material / coagulation material / blood material sample # time (%) time blood time gm / ml blank 1 100 0 : 17 : 27 0 : 17 : 24 2 0 : 15 : 25 3 0 : 19 : 33 naalo 2 1 3 . 60 0 : 00 : 38 0 . 4020 0 : 00 : 58 0 . 3865 2 0 : 00 : 30 0 . 3746 3 0 : 00 : 25 0 . 4447 cao 1 22 . 53 0 : 03 : 56 0 . 3371 0 : 04 : 02 0 . 2996 2 0 : 04 : 11 0 . 3533 3 0 : 03 : 35 0 . 3585 [ 0069 ] table 9 test 8 , day 3 , dog blood from dog 1 relative average average ratio average test coagulation coagulation material / coagulation material / blood material sample # time (%) time blood time gm / ml blank 1 100 0 : 05 : 53 0 : 05 : 54 2 0 : 05 : 36 3 0 : 06 : 10 naalo 2 1 9 . 53 0 : 00 : 34 0 . 3703 0 : 00 : 37 0 . 3413 2 0 : 00 : 34 0 . 3849 3 0 : 00 : 30 0 . 3847 cao 1 6 . 13 0 : 00 : 22 0 . 3966 0 : 00 : 24 0 . 3654 2 0 : 00 : 21 0 . 4442 3 0 : 00 : 20 0 . 3801 [ 0070 ] table 10 test 9 , day 3 , dog blood from dog 2 relative average average ratio average test coagulation coagulation material / coagulation material / blood material sample # time (%) time blood time gm / ml blank 1 100 0 : 05 : 54 0 : 05 : 37 2 0 : 06 : 04 3 0 : 06 : 01 naalo 2 1 10 . 73 0 : 00 : 38 0 . 3906 0 : 00 : 42 0 . 4039 2 0 : 00 : 38 0 . 3961 3 0 : 00 : 34 0 . 3718 cao 1 9 . 98 0 : 00 : 35 0 . 4646 0 : 00 : 17 0 . 4672 2 0 : 00 : 51 0 . 4154 3 0 : 00 : 38 0 . 5110 [ 0071 ] table 11 test 10 , day 3 , dog blood from dog 3 relative average average ratio average test coagulation coagulation material / coagulation material / blood material sample # time (%) time blood time gm / ml blank 1 100 0 : 07 : 42 0 : 04 : 43 2 0 : 09 : 10 3 0 : 09 : 13 naalo 2 1 8 . 59 0 : 00 : 40 0 . 3599 0 : 00 : 38 0 . 3247 2 0 : 00 : 42 0 . 3862 3 0 : 00 : 39 0 . 3689 cao 1 8 . 87 0 : 00 : 41 0 . 3825 0 : 00 : 48 0 . 3734 2 0 : 00 : 51 0 . 3782 3 0 : 00 : 24 0 . 3959 [ 0072 ] table 12 test 11 , day 4 , horse blood from horse 2 average relative average test coagulation coagulation coagulation average ratio material / blood material sample # time time time % material / blood gm / ml blank 1 0 : 41 : 22 0 : 41 : 19 100 2 0 : 41 : 17 zsm - 5 1 0 : 01 : 24 0 : 01 : 22 3 . 3 0 . 3849 0 . 3828 2 0 : 01 : 23 0 . 3997 3 0 : 01 : 20 0 . 3720 ( ca ) zsm - 5 1 0 : 03 : 33 0 : 04 : 16 10 . 3 0 . 3767 0 . 3744 2 0 : 04 : 01 0 . 3294 3 0 : 05 : 13 0 . 4263 [ 0073 ] table 13 test 12 , day 4 , dog blood from dog 1 average relative average test coagulation coagulation coagulation average ratio material / blood material sample # time time time % material / blood gm / ml blank 1 0 : 15 : 38 0 : 15 : 35 100 2 0 : 15 : 31 zsm - 5 1 0 : 01 : 30 0 : 01 : 25 9 . 1 0 . 3924 0 . 3679 2 0 : 01 : 25 0 . 3985 3 0 : 01 : 20 0 . 4108 ( ca ) zsm - 5 1 0 : 01 : 41 0 : 02 : 00 12 . 8 0 . 3809 0 . 4333 2 0 : 02 : 03 0 . 4182 3 0 : 02 : 15 0 . 2913 [ 0074 ] comparative table 14 relative coagulation time (%) at specified test material / blood ratio ( gm / ml ) horse blood cow blood dog blood mixed material 0 . 4 g / ml 0 . 2 g / ml 0 . 1 g / ml 0 . 05 g / ml 0 . 4 g / ml 0 . 4 g / ml avitene 8 . 2 sio 2 / naaio 2 4 . 8 3 . 4 29 . 02 oms - 2 ( ca )/ 1 . 9 4 . 9 20 . 16 naaio 2 mgo / naaio 2 3 . 4 1 . 7 32 . 5 cao / naaio 2 4 . 3 4 . 2 12 . 1 ceo / naaio 2 4 . 1 37 . 0 1 . 31 ca - zeolitey / 3 . 8 3 . 5 26 . 2 y / naaio 2 [ 0075 ] comparative table 15 relative coagulation time (%) horse blood cow blood dog blood material 0 . 4 g / ml 0 . 2 g / ml 0 . 1 g / ml 0 . 05 g / ml 0 . 4 g / ml 0 . 4 g / ml avitene 8 . 2 oms - 2 ( k ) 59 . 4 117 . 8 137 . 0 oms - 2 ( ca ) 3 . 3 , 6 . 6 18 . 6 106 . 8 8 . 2 , 4 . 0 7 . 0 zeolite y ( na ) & gt ; 100 & gt ; 100 & gt ; 100 zeolite y ( ca ) 9 . 7 , 30 . 7 16 . 5 36 . 9 35 . 4 , 56 36 . 2 ca - ol - 1 11 . 2 & gt ; 100 49 . 0 zeolite zsm - 5 3 . 3 9 . 1 zeolite zsm - 5 10 . 3 12 . 8 ( ca ) montmorillonite ca - 21 . 5 23 . 7 20 montmorillonite mordenite [ 0076 ] comparative table 15 relative coagulation time (%) horse blood cow blood dog blood material 0 . 4 g / ml 0 . 2 g / ml 0 . 1 g / ml 0 . 05 g / ml 0 . 4 g / ml 0 . 4 g / ml ca - mordenite 41 . 9 26 . 7 zeolite 4a ( na ) & gt ; 100 273 372 commercial sample zeolite 4a ( na ) 363 . 8 420 . 5 338 . 5 lab sample [ 0077 ] comparative table 16 relative coagulation time (%) horse blood cow blood dog blood material 0 . 4 g / ml 0 . 2 g / ml 0 . 1 g / ml 0 . 05 g / ml 0 . 4 g / ml 0 . 4 g / ml avitene 8 . 2 silica gel 2 . 5 , 1 . 8 , 1 . 6 6 . 95 , 1 . 97 10 . 34 9 . 27 19 . 6 , 5 . 9 8 . 1 ( sio 2 ) 1 chromosorb 2 . 6 47 . 1 paw - dmcs silica gel w / o 2 . 8 dehydration 1 chromatographic 4 . 7 silica gel 1 acid silica 13 . 5 ( sio 2 nh 2 o oms - 2 ( ca ) 3 . 3 , 6 . 6 18 . 6 106 . 8 8 . 2 , 4 . 0 7 . 0 naalo 2 3 . 8 12 . 1 , 3 . 1 6 . 9 , 19 . 7 mgo 3 . 9 & gt ; 100 12 . 3 , 4 . 7 109 . 7 cerium oxide 22 . 4 18 . 3 , 24 27 cao 6 . 1 8 . 4 12 . 3 , 4 . 7 14 . 1 p 2 o 5 6 . 2 6 . 4 ca - zeolite y 9 . 7 , 30 . 7 16 . 5 36 . 9 35 . 4 , 56 36 . 2 chabazite 2 . 1 , 46 . 2 76 . 8 89 . 5 ca - ol - 1 11 . 2 & gt ; 100 49 . 0 zeolite zsm - 5 3 . 3 30 . 9 9 . 1 ca - montmorillonite 21 . 5 23 . 7 20 bismuth oxide 20 . 9 46 . 3 silica alumina 21 . 3 48 . 6 activated 27 . 0 29 . 0 43 . 3 carbon zeolite 5a 41 . 3 , 23 . 2 68 . 8 52 . 9 69 . 87 26 . 4 , 31 39 . 7 zeolite rho 30 . 5 23 . 2 ca - mordenite 41 . 9 26 . 7 ferric oxide 31 . 9 chromosorb 101 34 . 2 yttrium oxide 37 . 6 cobalt oxide 38 . 3 calcium 38 . 3 phosphate chromosorb 102 41 . 2 stannic oxide 68 . 13 yunnan white 78 . 1 medicine nickel oxide 85 . 7 copper oxide 91 . 5 yb 2 o 3 97 . 3 oms - 2 ( k ) 59 . 4 117 . 8 137 . 0 alumina gel 82 . 3 161 131 zeolite na - y & gt ; 100 & gt ; 100 & gt ; 100 zeolite 4a ( na )- & gt ; 100 273 372 commercial sample ldh 157 . 4 165 . 6 coffee powder 261 . 8 zeolite 4a ( na )- lab 363 . 8 420 . 5 338 . 5 ca - zsm - 5 10 . 3 & gt ; 100 12 . 8 silica - calcia & gt ; 100 & gt ; 100 calcium & gt ; 100 chloride barium oxide & gt ; 100 aluminum & gt ; 100 oxide zinc oxide & gt ; 100 vanadium & gt ; 100 pentoxide titanic oxide & gt ; 100 zirconium & gt ; 100 oxide scandium & gt ; 100 oxide europium & gt ; 100 oxide lialo 2 & gt ; 100 blood coagulation accelerator materials were coated onto porous flexible substrates ; non - porous flexible substrates ; and rigid substrates . the coated substrates were prepared using the following procedures . a solution of ( na ) zeolite y was prepared . a swatch of cotton fabric approximately two centimeters ( cm ) by two centimeters ( cm ) was submerged in the solution without agitation for 5 minutes . the soaked swatch was removed from the solution , placed in an autoclave set at 100 ° c . and heated for a first time period of 24 hours . after the first heating period , the coated swatch was washed in distilled water and dried in an oven set at 100 ° c . for a second time period of 24 hours . a film of siloxane was prepared by applying room temperature vulcanizing silicone sealant ( 500 rtv high heat rutland silicone sealer available from rutland products , rutland vt .) over a non - stick surface . the film was allowed to cure at room temperature and in air for 24 hours , after which the 20 cured film was removed from the surface , washed in water and dried with a paper towel . a gel of zeolite 4a was prepared as previously described . the prepared zeolite 4a gel was applied to the cured siloxane film using a spatula . the coated siloxane film was placed into a fluorocarbon bottle and heated at 80 ° c . for 3 hours . the resulting coated siloxane film was washed with distilled water and dried at 100 ° c . for 4 hours . in a first test , sodium aluminum oxide was mixed individually with other blood coagulation accelerator materials in water to create a paste . each paste mixture was coated on a wooden substrate and dried at 100 ° c . for 24 hours . in a second test , polyvinyl acetate ( flexbond 153 emulsion , available from air products and chemicals incorporated of allentown , pa .) was applied onto a wooden substrate and allowed to stand for approximately 15 minutes . blood coagulation accelerator materials were sprinkled liberally onto individual polyvinyl acetate coated wooden substrates at the end of the 15 minute period . excess particles of the material were shaken off and the coated substrate was dried at 100 ° c . for 24 hours . in both tests , the coating strengths were determined by weighing the dried substrate and blood coagulation accelerator material , applying adhesive tape over the coating , peeling the tape off , and recording the weight of the blood coagulation accelerator material and test substrate remaining after the tape had been peeled off . no weight change was observed for coatings of ( ca ) zms - 5 , zeolite 5a or silica gel , indicating no material was removed from the substrate . in every case it was found that blood coagulation accelerator materials could be successfully coated onto a substrate . small packages or sachets each containing a blood coagulation accelerator material were prepared . each package was approximately 1 . 5 cm by 1 . 5 cm and comprised an outer cover or wrap of a nonwoven material enclosing about 0 . 1 gm of blood coagulation accelerator material . the package functioned as a support for the blood coagulation accelerator material . kimwipes ex - l wiper material available from kimberly - clark was found suitable for use as the nonwoven material . the packages with blood coagulation accelerator material enclosed within were dried at 100 ° c . for 24 hours . after drying , the packages were individually sealed in glass vials until use . anesthetized rats were used as test subjects . the three nails of each rear foot were simultaneously clipped at the juncture of the nail and the skin to induce bleeding . the nails of the left foot were left untreated while the nails of the right foot were treated with a blood coagulation accelerator material as described below . just prior to nail clipping , a vial containing a dried sachet of blood coagulation accelerator material was opened . after the nails were clipped , the sachet was opened and the nails of the right foot were inserted into the sachet and immersed in the blood coagulation accelerator materials . the right foot was removed from the sachet at 30 - second intervals and the clotting processes of the untreated ( left foot ) and treated ( right foot ) nails were observed . blood - clotting time , e . g . cessation of bleeding , was noted , and a relative and average relative clotting time were calculated from the data . the results of the testing are shown in table 17 . as shown by the results , the treatment with the blood coagulation accelerators substantially shortened the time required for bleeding to stop in each test . while preferred embodiments of the foregoing invention have been set for purposes of illustration , the foregoing description should not be deemed imitation of the invention herein . accordingly , various modifications , adaptations and alternatives may occur to one skilled in the art without departing the spirit and scope of the present invention .
a material for the enhancement of blood coagulation . the material comprises calcium cations , an inorganic oxide , a clay , an inorganic oxide in combination with calcium cations , a zeolite in combination with calcium cations , a zeolite in combination with an inorganic oxide and combinations thereof . the material when combined with blood reduces the coagulation time of the blood . also , a method for using the material to promote blood coagulation .