in the following detailed description , reference is made to the accompanying drawings , which form a part hereof and show by way of illustration specific embodiments in which the invention may be practiced . these embodiments are described in sufficient detail to enable those skilled in the art to practice the invention , and it is to be understood that other embodiments may be utilized , and that structural , logical , and electrical changes may be made without departing from the spirit and scope of the present invention . the progression of processing steps described is exemplary of the embodiments of the invention ; however , the sequence of steps is not limited to that set forth herein and may be changed as is known in the art , with the exception of steps necessarily occurring in a certain order . now referring to the figures , where like numerals designate like elements , fig1 is a flowchart of an exemplary method in accordance with the invention . in this embodiment of the invention , in step 110 , blood is obtained from a patient . in step 120 , the blood is incubated in a sterile container 200 ( fig2 a , 2 b , and 2 c ) at a temperature suitable to induce production of prophylactically or therapeutically active proteins ( e . g ., il - 1ra , interleukin 4 , interleukin 10 , and tgf beta ). the sterile container 200 can be of any suitable size or configuration ( e . g ., vial , capped container , closed tube ) and is preferably made of a material that can be subjected to sterilization ( e . g ., chemical sterilization , autoclave ). for example , the container 200 can be made of any suitable glass , ceramic , or plastic material ( e . g ., polystyrene , polypropylene ). the blood is incubated from about 35 to 39 ° c ., more preferably from about 36 ° c . to about 38 ° c ., most preferably at 37 ° c . the blood is incubated for a period of time suitable to produce a sufficient quantity of the therapeutically active protein to treat the patient . the blood is incubated in the sterile container from about 6 to about 36 hours , more preferably from about 10 to about 24 hours , most preferably for about 12 hours . in step 130 , components of the blood are separated following incubation of the blood . in a preferred embodiment , blood cells ( e . g ., monocytes ) are separated from the serum by any suitable method ( e . g ., centrifugation ). in step 140 , the protein serum component containing the therapeutically or prophylactically active proteins produced during incubation is removed . a determination is made at step 150 whether treatment of the patient is imminent . if so , at step 160 , the patient is treated with the serum . if not , the protein serum component is divided ( step 170 ) and stored in a container ( step 180 ). then , at step 190 , the container is frozen to preserve the serum for long or short term storage and / or for transportation of the serum until the determination is made that the patient should be treated ( repeat step 150 ). as a specific , but non - limiting example , 10 to 60 cc of blood is drawn ( step 110 ) from the patient and incubated at about 37 ° c . for about 12 hours in a sterile container having a modified inner surface to increase surface area and monocyte adherence ( step 120 ). the blood is centrifuged to separate the protein serum from the blood cell layer ( step 130 ). the protein serum is then removed ( step 140 ) from the sterile container . since , in this example , the patient is not to be treated near the time of removal ( step 150 ), the serum is placed , for example , into multiple containers or vials ( steps 170 and 180 ). the serum is deep frozen for storage within about 24 hours ( step 190 ). upon a determination that the treatment will be made ( step 150 ), the serum is thawed . after thawing , a portion of the serum ( e . g ., one container or vial ) of serum is administered ( e . g ., via injection or perfusion ) by an orthopedic surgeon into a patient , for example , at a specific site ( e . g ., specific joint , tendon , muscle , or other soft tissue ) to treat or reduce the symptoms associated with a disease condition ( e . g ., osteoarthritis or tendonitis ) ( step 160 ). fig2 a , 2 b , and 2 c illustrate a sterile container 200 modified in accordance with the invention . in this example , the sterile container 200 is a test tube . the sterile container 200 is modified in order to increase the surface area of the inner surface 210 of the container 200 . for example , as shown in fig2 a , the inner surface 210 of the sterile container 200 , or a portion thereof , can be treated with a corrosive agent ( e . g ., acid such as chromosulfonic acid ) resulting in the etching 220 of the inner surface 210 of the container 200 . alternatively , as shown in fig2 b , the surface area of the inner surface 210 of the sterile container 200 , or a portion thereof , can be increased by adding and / or coating the inner surface 210 of the container 200 with granules 230 ( e . g ., made of glass or plastic ) or other suitable materials ( e . g ., gels , wool , spheres , and particles ). without being bound by theory , it is believed that the increased surface area provides additional attachments for adherence by blood monocytes ( not shown ), which stimulates the monocytes to produce therapeutically or prophylactically active proteins such as , for example , il - 1ra . in one embodiment , as shown in fig2 c , the inner surface 210 of the container 200 , or a portion thereof , can be coated with an anti - coagulant 240 such as heparin . while the invention has been described in detail in connection with exemplary embodiments known at the time , it should be readily understood that the invention is not limited to such disclosed embodiments . rather , the invention can be modified to incorporate any number of variations , alterations , substitutions or equivalent arrangements not heretofore described , but which are commensurate with the spirit and scope of the invention . thus , the invention is not to be seen as limited by the foregoing description , but is only limited by the scope of the appended claims .
a method of producing blood serum containing prophylactically or therapeutically active proteins , including obtaining blood from a patient , incubating the blood at a suitable temperature to induce production of prophylactically or therapeutically active proteins , and removing the prophylactically or therapeutically active proteins from the blood .