for the purpose of promoting an understanding of the present invention , references are made in the text to exemplary embodiments of a system and method for the physico - chemical alteration of penicillin - binding proteins in penicillin - resistant gram - positive and gram - negative bacteria to induce sensitivity to otherwise ineffective penicillins , only one of which is described herein . it should be understood that no limitations on the scope of the invention are intended by describing these exemplary embodiments . one of ordinary skill in the art will readily appreciate that alternate but functionally equivalent use of compounds , solvents , concentrations , ph , and methods may be used to expand biochemical target reactivity and accessibility to reactive drugs and anti - toxins . the inclusion of additional elements , such as drugs and anti - toxins , depending upon the specific biochemical targets and conditions involved , may be deemed readily apparent and obvious to one of ordinary skill in the art . specific elements disclosed herein are not to be interpreted as limiting , but rather as a basis for the claims and as a representative basis for teaching one of ordinary skill in the art to employ the present invention . moreover , the terms “ substantially ” or “ approximately ” as used herein may be applied to modify any quantitative representation that could permissibly vary without resulting in a change in the basic function to which it is related . the physico - chemical aspect of the ss , less penicillin , is conceived upon knowledge of water activity relative to biological macromolecular rearrangements , and the knowledge that temperature and ph also affect these rearrangements . the specifics of the ss , less penicillin , are taken from the extensive hofmeister series for enhancing reversible denaturation of macromolecules in gram - positive bacteria ( e . g ., methicillin - resistant staphylococcus aureus ) and gram - negative bacteria ( e . g ., pseudomonas aeruginosa ). the hofmeister series is comprised of compounds known to effect water activity and stability of a substantial and varied range of macromolecules . the physico - chemical conditions of reversible denaturation rearrange biological macromolecules , thereby altering target motifs in protein , inhibiting structure - specific protein activities , altering passive diffusion through structural barriers , and imposing a temporal static state upon many metabolic processes , thereby improving outcome from coincident therapeutic treatment of targets . that is , such a disrupted and static condition in bacteria can be used as an advantage by including one or more different drugs or anti - toxins in this denaturing solution ; drugs and anti - toxins can include a range of known biochemical agents , such as penicillin , antiseptics , or disinfectants selected for compatibility with normal tissue at the site of topical application . in this embodiment , cloxacillin , an otherwise substantially ineffective penicillin , was chosen and proved to be highly efficient in directly killing bacteria normally resistant to penicillin . fig1 through 41 illustrate the efficiency of a ss formulation derived from members of the hofmeister series for physico - chemically inducing alteration of in situ target proteins to establish sensitivity of gram - positive ( i . e ., mrsa ) and gram - negative bacteria ( i . e ., pseudomonas aeruginosa ) to otherwise ineffective penicillins . the ss formulation at ph 7 . 4 , but not ph 5 . 5 , is effective in reversing penicillin - resistance in a methicillin - resistant strain of staphylococcus aureus ( mrsa ) and in pseudomonas aeruginosa , important bacterial pathogens . the ss affects water activity , which in turn alters the macromolecular structure of the target . in this manner , penicillin - resistant proteins are rearranged by physico - chemical conditions such that covalent binding of penicillins is allowed and the structures of penicillin transport proteins are altered or thereby inactivated to defeat the function of penicillin efflux . in an exemplary embodiment for treatment of planktonic bacteria , the ss contains high , at times saturated , concentrations of phosphate , sulfate , and acetate anions , potassium and ammonium cations , plus a small concentration of free ammonia , all prepared in water , and applied at 22 ° c . or 35 ° c . for example , in one embodiment , the molar concentrations of the phosphate , sulfate , and acetate anions may be 3 . 1 m , 0 . 4 m , and 0 . 2 m , respectively , and the potassium and ammonium cations may be 2 . 6 m and 3 . 7 m , respectively . in an exemplary embodiment , ammonium hydroxide is added to the ss in order to bring the ss to the desired ph ( e . g ., ph 7 . 4 ), resulting in a small amount of free ammonia . in various other embodiments , sulfuric acid , acetic acid , a combination thereof , or another acid is added to lower the ph of the ss . in various embodiments , each compound may have a molar concentration ranging from 0 . 01 m to 4 . 0 m . in addition , the ss may include any of the compounds in the hofmeister series ; the specific formula of the ss may be adjusted for maximal physico - chemical effectiveness in each specific application . in various physico - chemical embodiments , the ph , concentrations , solvents and / or temperature of the formulations may vary in order to maximize the effectiveness of macromolecular rearrangements and the outcome for different pathological biomolecular targets , all with regard for the tolerance of the normal tissue involved in topical applications . for example , the temperature may range from freezing to 43 ° c ., the limiting temperature for thermal pain sensation . in various embodiments , the solvent ( i . e ., water ) may be blended with or replaced with a substance ( s ) that is known to affect water activity and macromolecular rearrangements , such as alcohols ( e . g ., ethanol , propanol , butanol ) or aprotic solvents ( e . g ., dimethyl acetamide , dimethyl formamide , dimethyl sulfoxide ). in an exemplary embodiment , the ss is prepared to an effective ph of 7 . 4 . in various other embodiments , the ss may be prepared to a ph ranging from 3 . 5 to 10 . the ph may be varied to maximize the effectiveness of the macromolecular rearrangements and the outcome for different pathological biomolecular targets . in an exemplary embodiment , the ss formulation , often acting as a humectant , is dictated by the alteration of water activity that leads to reversible denaturation of biomolecules , both those that are targets and those affecting accessibility to targets , thereby maximizing the effectiveness of the macromolecular arrangements and the resulting outcome . in various embodiments , the formulation of ss may be varied by the addition of additives that affect the water activity of biomolecules . these additives are taken from the hofmeister series , and include chaotropes and kosmotropes , including humectants characterized as astringents or styptics , such as alum , burow &# 39 ; s solution ( i . e ., aluminum acetate ), and silver nitrate . these additives are used to effect the reversible denaturation of biomolecules resulting in effective presentation of a variety of new biomolecular targets to drugs or anti - toxins , such as silver nitrate , contained within the topical solutions . fig1 through 17 illustrate kill curve time course experiments of stationary and logarithmic - phase mrsa bacterial suspensions exposed to pbs as well as denaturing ss at ph 5 . 5 or ph 7 . 4 in the presence of cloxacillin concentrations spanning from 512 μg / ml to 4096 μg / ml . experiments were conducted at 22 ° c . or 35 ° c . the completion time for the experiments ranged from 20 minutes to 24 hours . for these experiments , the mrsa strain was grown overnight in mueller - hinton broth ( mh )+ 2 % nacl at 35 ° c ., subcultured into fresh media , and grown to mid - logarithmic phase . the cells were centrifuged and resuspended in ph 7 . 4 mh + 2 % nacl to produce an od 600 nm known to indicate logarithmic - phase or stationary - phase of growth . an appropriate volume of cells was added to 1 ml of buffered ss in a 10 ml falcon tube to yield a final concentration of ca . 1 × 10 8 colony forming units ( cfu )/ ml , except for the experiments of fig1 - 4 where the final concentration of ca . 1 × 10 6 cfu / ml applies . survival was determined for bacteria held for varied exposure times of up to 24 hours in the ss or in pbs at ph 5 . 6 or ph 7 . 4 , each altered with cloxacillin concentrations ranging in doublings from 512 μg / ml to 4096 μg / ml . survival was measured from samples held at 22 ° c . and 35 ° c . after exposure , samples of 50 μl were then withdrawn and a serial dilution series established . cells were then plated on mh + 2 % nacl agar plates and incubated at 22 ° c . or 35 ° c . for cfu formation , after which colonies were counted , surviving fractions recorded , and survival curves constructed . triplicate experiments were conducted for each exposure condition . the cells were treated under static conditions in all cases and treatments were always made on cells held in salt solutions , never on cells held in growth media . in addition to logarithmic - phase cells , stationary - phase cells removed from growth cycle were also treated . cells exposed to ss at ph 7 . 4 in the presence of cloxacillin are killed . killing is more efficient for logarithmic - phase cells than for stationary - phase cells . both logarithmic - phase and stationary - phase cells are killed more effectively by treatment at 35 ° c . than treatment at 22 ° c . killing efficiency is evaluated as therapeutically sufficient according to the 10 − 5 level of killing in 5 minutes specified by the british standard bs en 1276 : 1997 chemical disinfectants and antiseptics , which is referred to as the gold standard for efficacy of bactericidal agents . typically , agents meeting the british standard are too toxic for human topical application . however , this is not a restriction for effective ss containing cloxacillin used in the illustrated embodiment . as developed in fig1 through 4 , substantial killing of static logarithmic - phase mrsa by cloxacillin is achieved at ph 7 . 4 at 22 ° c . for ss only . pbs at ph 5 . 5 or at ph 7 . 4 is not effective , and neither is ss at ph 5 . 5 . regarding the results shown in fig4 , no survivors were seen on any of the treated plates ; therefore , the surviving fraction for all time points is less than the 2 × 10 − 4 indicated as a conservative maximum allowed for the dilution factor plated . experiments were also conducted on mrsa logarithmic - phase cells with ss alone at 22 ° c . no killing effect was seen at ph 5 . 6 or ph 7 . 4 for ss alone . as fig5 through 9 illustrate , the efficiency of killing of stationary - phase mrsa cells at 22 ° c . does not provide the 10 − 5 level of killing in 5 minutes specified by the british standard . however , at the highest concentration of cloxacillin , that is , 4096 μg / ml , the 10 − 5 level of killing is achieved in 3 hours , as detailed in fig9 . as shown in fig1 through 13 , killing is enhanced by exposure of logarithmic - phase mrsa to cloxacillin at 35 ° c . as fig1 through 17 illustrate , the enhanced killing extends to stationary cells exposed at 35 ° c . as well . the efficiency of killing of both logarithmic - phase and stationary - phase mrsa cells at 35 ° c . approaches or exceeds the 10 − 5 level of killing in 5 minutes specified by the british standard . the level of killing is concentration dependent and is based upon the conditions of the treatment , such as species and initial sensitivity / resistance , whether the cells are in a stationary or logarithmic growth phase , temperature , and specific hofmeister series compounds used . fig1 through 41 illustrate kill curve time - course experiments of stationary - phase and logarithmic - phase pseudomonas aeruginosa bacterial suspensions exposed to pbs , to ss , to pbs in the presence of cloxacillin concentrations spanning from 512 μg / ml to 4096 μg / ml , or to ss in the presence of cloxacillin concentrations spanning from 512 μg / ml to 4096 μg / ml . experiments were conducted at 22 ° c . or 35 ° c . over the course of 10 , 20 , or 60 minutes . pseudomonas aeruginosa cells were prepared as described above for mrsa with a few exceptions . the pseudomonas aeruginosa cells were first grown in trypticase soy broth and then frozen in aliquots , which were later rescued into mh broth + 2 % nacl for use as in the mrsa experiments . an appropriate volume of cells was added to 1 ml of comparative salt solutions in a 10 ml falcon tube to yield a final concentration of ca . 1 × 10 8 colony forming units ( cfu )/ ml . survival was determined for bacteria held for varied exposure times of up to 60 minutes in pbs , in ss , in pbs altered with cloxacillin concentrations ranging in doublings from 512 μg / ml to 4096 μg / ml , or in ss containing cloxacillin concentrations ranging in doublings from 512 μg / ml to 4096 μg / ml . the salt solutions were compared at a ph of 5 . 6 or 7 . 4 . survival was measured from samples held at 22 ° c . or 35 ° c . after exposure , samples of 50 μl were then withdrawn and a serial dilution series established . cells were then plated on mh + 2 % nacl agar plates and incubated at 22 ° c . or 35 ° c . for cfu formation , after which colonies were counted , surviving fractions recorded , and survival curves constructed . triplicate experiments were conducted for each exposure condition . the cells were treated under static conditions in all cases and treatments were always made on cells held in salt solutions , never on cells held in growth media . in addition to logarithmic - phase cells , stationary - phase cells removed from growth cycle were also treated . fig1 through 21 show the results of the control experiments . for the control experiments , logarithmic - phase pseudomonas aeruginosa cells were treated in pbs ( fig1 ) or ss ( fig1 ), and stationary - phase pseudomonas aeruginosa were treated in pbs ( fig2 ) or ss ( fig2 ). all experiments were conducted at both 22 ° c . and 35 ° c . for 60 minutes . no substantial killing was observed for any of these controls . fig2 through 25 illustrate the non - substantial killing logarithmic - phase pseudomonas aeruginosa using a pbs solution with cloxacillin concentrations ranging from 512 μg / ml to 4096 μg / ml at ph 7 . 4 . the experiments were conducted at 22 ° c . for 20 minutes . fig2 through 29 illustrate the efficiency of killing logarithmic - phase pseudomonas aeruginosa using ss with cloxacillin concentrations ranging from 512 μg / ml to 4096 μg / ml at ph 7 . 4 . for cloxacillin concentrations of 2048 μg / ml and 4096 μg / ml , the experiments were conducted at 22 ° c . for 20 minutes . for cloxacillin concentrations of 512 μg / ml and 1024 μg / ml , the experiments were conducted at 22 ° c . for 10 minutes . at all concentrations of cloxacillin , the ss , but not the pbs solution , was substantially efficient in killing pseudomonas aeruginosa . fig3 through 33 illustrate the non - substantial killing of logarithmic - phase pseudomonas aeruginosa using a pbs solution with cloxacillin concentrations ranging from 512 μg / ml to 4096 μg / ml at ph 7 . 4 . the experiments were conducted at 35 ° c . for 20 minutes . fig3 through 37 illustrate the substantial killing of logarithmic - phase pseudomonas aeruginosa using the ss with cloxacillin concentrations ranging from 512 μg / ml to 4096 μg / ml at ph 7 . 4 . the experiments were conducted at 35 ° c . for 20 minutes . fig3 through 41 illustrate the substantial killing of stationary - phase pseudomonas aeruginosa using ss with cloxacillin concentrations ranging from 512 μg / ml to 4096 μg / ml at ph 7 . 4 . the experiments were conducted at 22 ° c . for 20 minutes . killing is more efficient for cells exposed to higher concentrations of cloxacillin . in the embodiments shown , the highest concentration of cloxacillin tested was 4096 μg / ml ; however , it is not necessarily the maximum effective or tolerated concentration . the concentration of cloxacillin or other drug may be varied depending on the contact effect required for topical applications in any given situation . that is , the preferred concentration should be determined for each specific application . the use of various concentrations of compounds from the hofmeister series can affect macromolecular hydration and protein denaturation , which may expose novel penicillin - binding amino acid motifs of pbps and other non - specific proteins . in addition , high salt concentrations inactivate efflux transporters and porins . as a result , both penicillin - resistant gram - positive and gram - negative pathogenic bacteria may be created as penicillin - sensitive by physico - chemical denaturation induced by exposure to the embodied ss and related salt solutions when containing penicillin . it is expected that under these specific conditions , universal creation of protein target sensitivity to penicillin results from such physico - chemical treatment , that is , a field effect of covalent binding of proteins to penicillin is established during the physico - chemical treatment by ss in the presence of penicillin . this field effect , whereby any affected bacterial proteins may be modified to enhance penicillin - binding , promotes direct killing and allows , for the first time , the potential application of penicillin to control static cells , including stationary - phase cells , as well as actively growing cultures of bacteria . penicillin dissolves well in the ss described and may be used for treatment in the form of a topical application . cloxacillin at relatively high concentration in the ss substantially kills static logarithmic - phase and static stationary - phase penicillin - resistant mrsa and pseudomonas aeruginosa cells rapidly upon exposure at room temperature , and the degree of killing is advanced using variables of ph and temperature . this substantial killing of static penicillin - resistant bacteria at room temperature is notable in that the killing effectiveness is established in both logarithmic - phase and stationary - phase gram - positive and gram - negative bacteria . the embodiment of discovery and application described for physico - chemically induced alteration of in situ target proteins producing creation of sensitivity to otherwise ineffective penicillin is of great value in regard to both concepts and applications for continuing efforts to remedy clinical penicillin - resistance , and by extension a spectrum of related drug - resistance . the fact that penicillin - resistant static bacteria , both gram - positive and gram - negative , are killed directly using a topically innocuous solution immediately applies to topical treatments such as those involved in pre - surgical fields so that contaminating penicillin - resistant bacteria will be purged prior to surgical procedures , thus eliminating subsequent parenteral infections that currently plague clinical invasive procedures . topical applications include , but are not limited to skin colonization , suppurating infections , infections of the eye , nares , throat and mouth , wounds , burns , encysted infections , cellulitis , fulminating fasciitis , and also bacterial infection upon internal structures , such as the vagina , urinary tract and bladder , peritoneal cavity , large intestine , lung and trachea , stomach , etc ., all of which are accessible to instillation procedures . in addition , bacterial infections are a complicating factor in space travel . injuries and radiation burns do not heal as well in space , resulting in nagging injuries which may affect an astronaut &# 39 ; s performance . the present invention provides a new ability to fight infections in space . bacterial infections are also a major factor for the department of defense , especially during war times . often , injuries that occur in the field become infected with the subject bacteria and are barely treatable . use of the present invention provides an improved method for wound and burn healing . likewise , the department of homeland security is concerned about infections that result from radiation burns occurring at dirty bomb sites . the use of the present invention would provide a new and simple way of dealing with that issue . the present invention operates in physico - chemical mode , which exposes normally inaccessible target motifs for covalent binding of penicillin , thus demonstrating for the first time a mechanism of action for penicillin that is outside the dogma of biochemical action of penicillin upon only transpepidases in actively growing bacterial cells . the present embodiment claims rearrangement of macromolecules using compounds from the hofmeister series , thereby yielding new target motifs accessible by drugs and / or anti - toxins carried in the ss , which may be applied topically to manage resistant or problematic organisms and / or macromolecules . the scope of this discovery includes topical applications for treatment of bacteria ; viruses ; molds and yeasts ; topical parasites , such as ring - worm , ticks , and cutaneous and mucocutaneous leishmania ; and pathological macromolecules , such as bacterial exotoxins and endotoxins , snake and spider venoms , super - antigens , and prions . “ topical application ” includes application not only to skin , but also to cystic and suppurating infections , wounds , burns , nares , throat , mouth , as well as all instillations such as to the urinary tract and bladder , vagina , peritoneal cavity , large intestine , stomach , lung , and trachea . the mechanism of denaturation and rearrangement of macromolecules , and not only proteins , contributes to the disruption of pathologic processes by compounds of the hofmeister series , opening new accessibility to molecular target motifs for effective management by drugs , such as penicillins , and anti - toxins , such as silver ions , carried in specifically optimized ss . combinations of drugs and anti - toxins may be applied in this regard . for example , in topical applications of the ss to fulminating fasciitis , a condition requiring rapid and multiple reversals of currently resistant pathologic processes , penicillin may be included to kill bacteria and silver ions may be included to inactivate super - antigens . the present embodiment claims management of water activity and structure associated with macromolecules in bacterial pathogens by use of compounds of the hofmeister series , and examines the substantial killing effects due to new target motifs that react with penicillin in planktonic bacteria during exposures to the ss . topical clinical applications of the ss carrying drugs and / or anti - toxins will , however , encounter sessile , as well as planktonic bacteria , the principal additional barrier to accessibility by drugs and / or anti - toxins then being the biofilm of polysaccharides , mucopeptides , etc . secreted by and covering the sessile bacteria . it is expected that dehydration and denaturation by humectant - like and other compounds from the hofmeister series in the ss will permeabilize this highly hydrated biofilm to drugs and / or anti - toxins , as well as expose new target motifs and inactivate outer membrane - associated accessibility barriers as shown in the embodiment herein .
systems and methods for the use of compounds from the hofmeister series coupled with specific ph and temperature to provide rapid physico - chemical - managed killing of penicillin - resistant static and growing gram - positive and gram - negative vegetative bacteria . the systems and methods represent the more general physico - chemical enhancement of susceptibility for a wide range of pathological macromolecular targets to clinical management by establishing the reactivity of those targets to topically applied drugs or anti - toxins .