according to a first embodiment of the pharmaceutical composition of the present invention , a process of making polydatin aqueous solution for injection is provided . according to a first exemplary illustration of the first embodiment , a process of making an aqueous solution for injection is described , wherein the aqueous solution has a composition comprising a quantity of polydatin , a quantity of naoh aqueous solution at ph 8 . 5 and a quantity of 3 . 6 % nacl aqueous solution . according to the composition of the first exemplary illustration , the quantity of polydatin is 40 g , the quantity of naoh solution is 8 l , and the quantity of 3 . 6 % nacl is used for adjusting the total volume of the composition into a 10 l solution , wherein the composition of the first exemplary illustration is divided into 1000 unit . the process of making an aqueous solution for injection comprises the steps of dissolving 40 g polydatin in 8 l naoh aqueous solution at ph 8 . 5 ; adding a quantity of nacl until the total volume is 9 . 8 l ; adjusting the ph to 8 . 5 and adding nacl aqueous solution until the total volume is 10 l ; filtering using 0 . 22 μm microfilter membrane ; and dividing the solution into 1000 units and keeping in ampoules or bottles . according to a second exemplary illustration of the first embodiment , a process of making an aqueous solution for injection is described , wherein the aqueous solution has a composition comprising a quantity of polydatin , a quantity of naoh aqueous solution at ph 8 . 5 , a quantity of propandiol , a quantity of tween 80 , and a quantity of 3 . 6 % nacl aqueous solution . according to the composition of the first exemplary illustration , the quantity of polydatin is 80 g , the quantity of naoh solution is 6 l , the quantity of propandiol is 2 l , the quantity of tween 80 is 50 ml , and the quantity of 3 . 6 % nacl is used for adjusting the total volume of the composition into a 10 l solution , wherein the composition of the first exemplary illustration is divided into 1000 unit . the process of making an aqueous solution for injection in the embodiment 1 - 2 comprises the steps of dissolving 40 g polydatin in 2 l propandiol solution ; adding 50 ml tween 80 and naoh aqueous solution respectively and mixing ; adding a quantity of 3 . 6 % nacl until the total volume is 9 . 8 l ; adjusting the ph to 8 . 5 and adding nacl aqueous solution until the total volume is 10 l ; filtering using 0 . 22 μm microfilter membrane ; and dividing the solution into 1000 units and keeping in ampoules or bottles . according to a third exemplary illustration of the first embodiment , a process of making an aqueous solution for injection is described , wherein the aqueous solution has a composition comprising a quantity of polydatin , a quantity of anhydrous alcohol , propandiol , and a quantity of sodium carbon . according to the composition of the third exemplary illustration , the quantity of polydatin is 100 g , the quantity of anhydrous alcohol is 2250 ml , the quantity of propandiol is 1000 ml , and the quantity of sodium carbonate is used for adjusting the total volume of the aqueous solution into 5000 ml . the aqueous solution in the embodiment 1 - 3 is diluted 25 to 50 times by 0 . 9 % sodium fluoride or 5 % glucose injection solution for administration . the process of making an aqueous solution for injection in the embodiment 1 - 3 comprises the steps of dissolving 20 g polydatin in 450 ml anhydrous alcohol ; adding 100 ml buffer solution and mixing by ultrasound or stirring for 2 to 5 minutes such that the polydatin is dissolved ; adding 200 ml propandiol and the remaining buffer solution until the total volume is 1 l ; filtering using 0 . 22 μm microfilter membrane ; and refilling nitrogen and dividing the solution into 1000 units which are kept in brown ampoules or bottles . the buffer solution is prepared by mixing one unit of 0 . 1 mol / l sodium carbonate and nine units of 0 . 1 mol / l sodium hydrocarbonate . a process of making a cool dry powder of polydatin for injection . a process of making a polydatin solution is illustrated in embodiment 2 - 1 . the process of making a polydatin solution in the embodiment 2 - 1 comprises the steps of dissolving 40 g polydatin in 8 l ph 8 . 5 naoh aqueous solution ; adding a quantity of 5 % aqueous manitol solution until the total volume is 9 . 8 l ; adjusting the ph to 8 . 5 and adding 3 . 6 % nacl aqueous solution until the total volume is 10 l ; filtering using 0 . 22 um microfilter membrane ; and dividing the solution into 1000 units and keeping in vial for dry powder . a process of freeze dry is also illustrated in the embodiment 2 - 1 , comprising the step of obtaining the solution obtained from the process of making a polydatin solution , and placing the solution in a freeze dry condition with a temperature at − 35 ° c . such that the solution is kept in − 30 ° c . for 3 hours ; vacuum pumping at condensation temperature at − 40 ° c . and increasing the temperature to 40 ° c . gradually such that the frozen solution is increased gradually ; drying the frozen solution and that the freeze dry product is obtained . the product can then be packed and pressed with piston , enclosed and labeled as a polydatin injection powder . the powder is adapted for dissolving in physiological saline for injection use . a process of making a polydatin solution is illustrated in embodiment 2 - 2 . the process of making a polydatin solution in the embodiment 2 - 2 comprises the steps of dissolving 80 g polydatin in 8 l ph 9 . 5 naoh aqueous solution ; adding a quantity of 5 % aqueous manitol solution until the total volume is 9 . 8 l ; adjusting the ph to 8 . 5 and adding 3 . 6 % nacl aqueous solution until the total volume is 10 l ; filtering using 0 . 22 μm microfilter membrane ; and dividing the solution into 1000 units and keeping in vial for freeze dry powder . a process of freeze dry is also illustrated in the embodiment 2 - 2 , comprising the step of obtaining the solution obtained from the process of making a polydatin solution , and placing the solution in a freeze dry condition with a temperature at − 30 ° c . such that the solution is kept in − 30 ° c . for 3 hours ; vacuum pumping at condensation temperature at − 40 ° c . and increasing the temperature to 40 ° c . gradually such that the frozen solution is increased gradually ; drying the frozen solution and that the freeze dry product is obtained . the product can then be packed and pressed with piston , enclosed and labeled as a polydatin injection powder . the powder is adapted for dissolving in physiological saline for injection use . a process of making an injection solution of the present invention is illustrated in the embodiment 2 - 2 , comprising the steps of mixing 2 l propandiol and 500 ml 0 . 5 % hydrochloric acid ; adding a predetermined quantity of physiology saline such that the total volume is 10 l , filtering using 0 . 22 μm microfilter membrane ; and dividing the solution into 1000 units and keeping in vial . each of the vials comprises 10 ml of injection solution . a package comprising a 10 ml injection solution and a unit of freeze dry powder is preferred such that the freeze dry powder is capable of dissolving in the injection solution for injection . a process of making a composition of polydatin in tablet form . 1000 tablet each having a composition of 50 mg polydatin are prepared according to the routine making process , and that the composition of polydatin comprises a quantity of polydatin , a quantity of lactose , a quantity of starch , a quantity of polyethylene pyrrolidone k30 , and a quantity of magnesium stearate . in embodiment 3 , 50 g of polydatin , 107 g of lactose , 25 g of starch , 16 g of polyethylene pyrrolidone k30 , and 2 g of magnesium stearate are used and that the total weight is 200 g . the polydatin is mixed with lactose and starch to form a mixture . the mixture is transformed into a granule form by using polyethylene pyrrolidone k30 and passing through a no . 16 model unit . after drying , the granule is mixed with magnesium stearate and pressed into a predetermined shape . other polydatin in tablet form having different active composition of polydatin can be made by varying the ratio of the quantity of polydatin and vehicle , or the force of pressing device . a process of making a composition of polydatin in capsule form , comprising the steps of : mixing a quantity of polydatin , a quantity of lactose , a quantity of microcrystalized cellulose , and a quantity of magnesium stearate . in the embodiment 4 , 200 capsules having 50 mg active composition of polydatin are prepared from 10 g polydatin , 19 . 5 g lactose , 10 g microcrystalized cellulose , and 0 . 5 g magnesium stearate , wherein a total weight is 40 g . a process of making a composition of polydatin in ointment form , comprising the steps of : mixing and stirring a quantity of polydatin and a quantity of olive oil ; and adding a quantity of dissolved white vaseline while stirring ; and mixing thoroughly . the ointment is then canned . in embodiment 5 , a 1 % polydatin ointment is prepared from 1 g polydatin , 10 g olive oil , and 89 g white vaseline wherein the total weight is 100 g . a process of making a composition of polydatin in suppository form , comprising the steps of : mixing and stirring a quantity of polydatin and a quantity of glycerol ; and adding a quantity of dissolved glycerol gelatin while stirring ; and mixing thoroughly . the suppository is then canned . in embodiment 5 , a 1 % polydatin suppository is prepared from 1 g polydatin , 5 g glycerol , and 84 g glycerol gelatin wherein the total weight is 100 g . a treatment action for shock related to blood loss of a composition of polydatin . the observation is based on the experiment carried out comprising the steps of ( 1 ) anaesthetizing two groups , namely a treatment group and a control group , of sd rats with urethane ketamine , inducing bleeding by intubulation at femoral artery and recording blood pressure ; wherein femoral vein is used for medication and blood transfusion and cremasteric specimen is prepared by baez method ; ( 2 ) inducing bleeding from femoral artery such that an average arterial blood pressure is maintained between 5 . 1 to 5 . 6 kpa ; ( 3 ) administering a composition of polydatin injection through femoral vein after one hour wherein the dosage of the composition is 0 . 6 ml / kg , that the concentration of polydatin is 4 mg / ml and the dosage is 2 . 4 mg / kg in the treatment group and the composition of polydatin is replaced by physiological saline in the control group ; ( 4 ) conducting blood transfusion after 20 minutes such that the volume of blood loss due to bleeding is equal to the volume of blood loss due to blood transfusion ; and the records are made by using olympus microscope , hitachi monitoring system and physiological recording meter to observe and record microcirculation and blood dynamics . the survival period of each subject of the two groups are recorded . ( 1 ) survival period : a ratio of the survival time of the treatment group and the control group is larger than 5 . 5 , wherein 9 / 10 of the treatment group has a survival period of 24 hours , 8 / 10 of the treatment group has a survival period of 48 hours , and 6 / 10 of the treatment group has a survival period of 72 hours . all the subjects in the control group die within 24 hours . ( 2 ) blood pressure : after experienced shock for 1 hour , the average arterial blood pressure is 5 . 3 + 0 . 2 kpa . after 30 minutes of medication of polydatin , the arterial pressure is increased to 8 . 6 + 0 . 8 kpa in the treatment group , while there is no significant difference in blood pressure in the control group receiving physiological saline . ( 3 ) diameter of micro blood vessel : after experienced shock for 1 hour , the diameter of micro - aneurysm is reduced to 63 . 3 % of the diameter in average before shock . the diameter is then increased by 20 . 3 % after medication of polydatin and there is no significant difference of diameter in the control group . ( 4 ) number of opening capillaries : before shock , the number of open capillary is 5 . 1 ± 0 . 4 per μm 2 . after induced bleeding , the number of open capillary is decreased to 2 . 2 ± 0 . 6 per μm 2 . the number increased to 4 . 2 ± 0 . 5 per μm 2 in 10 minutes after polydatin medication . there is no significant difference of number of opening capillaries in the control group before and after the administration of physiological saline . a treatment action for shock related to skin burn of a composition of polydatin . the observation is based on the experiment carried out comprising the steps of : anaesthetizing two groups , namely a treatment group and a control group , of sd rats with urethane ketamine , burning about 35 % of body surface below waist for 30 seconds with 80 ° c . water ; administering 0 . 6 ml / kg polydatin injection ( comprising 4 mg / ml polydatin and the dosage is 2 . 4 mg / kg ) by venous injection after half hour of burning in the treatment group and replacing the polydatin injection by physiological saline in control group . result : the average survival period of the treatment group is longer than 13 . 3 hours while the average survival period of the control group is 7 . 0 ± 2 . 6 hours . one skilled in the art will understand that the embodiment of the present invention described above is exemplary only and not intended to be limiting . it will thus be seen that the objects of the present invention have been fully and effectively accomplished . it embodiments have been shown and described for the purposes of illustrating the functional and structural principles of the present invention and is subject to change without departure form such principles . therefore , this invention includes all modifications encompassed within the spirit and scope of the following claims .
the invention discloses pharmaceutical compositions containing polydatin or its pharmaceutically acceptable salts with the effect of improving microcirculation , and their application in preparing drugs which can improve microcirculation . the compositions are an non - gastrointestinal injection preparation , the preparation can be an aqueous solution which have ph 7 . 0 - 10 . 0 comprising 5 - 50 % propandiol . the dosage forms of the compositions may be lyophilizing powder injection , oral form , suppository of procto - administration and other forms of administrations comprising unguent , ointment , paste film coating and the like . the compositions may be used to treat and / or prevent diseases related to microcirculation blockage . the unit form may contain 1 - 1000 mg polydatin or its derivatives . the invention solves the problem of using polydatin to improve microcirculation . the composition of the invention can be used in treating shock , cardiac and cerebrovascular diseases , diseases of sense organs , diabetes complication , vaculitis related to blockage of blood vessel , hemorrhoid related to circulation obstruction , skin injury and burn .