the present invention involves an apparatus comprising a first container and a second container , and a conduit interconnecting the first container with the second container ; and having interposed between the first container and the second container , at least one porous medium including a leucocyte depletion medium , a red cell barrier medium , an assembly comprising a leucocyte depletion medium and a red cell barrier medium , or combinations thereof . in a preferred form of the filter of the subject invention , the fibers of which the filter element is composed are modified by grafting thereon a mixture of two monomers , one containing hydroxyl groups and another containing anionic groups , such as carboxyl groups , with the hydroxyl groups present in larger numbers . as described in u . s . pat . no . 4 , 880 , 548 , herein incorporated by reference , the filter media of this invention are preferably surface modified using a mixture comprising hydroxyl - terminated and carboxyl - terminated monomers . in a preferred form of this invention , the monomers are respectively hydroxyethyl methacrylate ( hema ) and methacrylic acid ( maa ), and the monomer ratios are preferably in the range ( carboxyl : hydroxyl ) of about 0 . 01 : 1 to about 0 . 5 : 1 , and more preferably in the range of about 0 . 05 : 1 to about 0 . 35 : 1 . a preferred monomer ratio is one which produces a desired zeta potential at the ph of plasma ( 7 . 3 ) of about - 3 to about - 30 millivolts , a more preferred ratio produces a zeta potential of about - 7 to about - 20 millivolts , and a still more preferred ratio produces a zeta potential of about - 10 to about - 14 millivolts . the cwst of the filter elements made with the pbt fibers according to this invention have a cwst as formed of about 50 to about 54 dynes / cm , and most or all other fibers which may be used have a cwst below 55 dynes / cm . surface grafting using the monomers noted above causes the cwst of the fibers to increase , the exact value obtained being dependent on the ratio of the two monomers . a preferred range for the cwst of the devices of this invention is about 70 to about 115 dynes / cm , a more preferred range is about 90 to about 100 dynes / cm and a still more preferred range is about 93 to about 97 dynes / cm , these ranges being obtained by varying the ratio of carboxyl - terminated and hydroxyl - terminated monomers . although the fibers of the porous medium may remain untreated , they are preferably treated to make them even more effective . for example , the fibers may be surface modified to increase the critical wetting surface tension ( cwst ) of the fibers . as disclosed in u . s . pat . no . 4 , 880 , 548 , the cwst of a porous medium may be determined by individually applying to its surface a series of liquids with surface tensions varying by 2 to 4 dynes / cm and observing the absorption or non - absorption of each liquid over time . the cwst of a porous medium , in units of dynes / cm , is defined as the mean value of the surface tension of the liquid which is absorbed and that of the liquid of neighboring surface tension which is not absorbed within a predetermined amount of time . the absorbed and non - absorbed values depend principally on the surface characteristics of the material from which the porous medium is made and secondarily on the pore size characteristics of the porous medium . liquids with surface tensions lower than the cwst of a porous medium will spontaneously wet the medium on contact and , if the medium has through holes , will flow through it readily . liquids with surface tensions higher than the cwst of the porous medium may not flow at all at low differential pressures and may do so unevenly at sufficiently high differential pressures to force the liquid through the porous medium . in order to achieve adequate priming of a fibrous medium with a liquid such as blood , the fibrous medium preferably has a cwst in the range of about 53 dynes / cm or higher . the number of carboxyl groups per unit of surface area appears to have an important effect on the adhesion of platelets to fiber surfaces . this effect is reflected in the proportion of platelets recovered in the filter effluent as a fraction of the number present in the platelets prior to filtration . platelet recovery peaks at the optimum proportion of maa . the number of carboxyl groups per unit of fiber surface is , over the range of interest of this invention , thought to be close to proportional to the amount of maa in the monomeric grafting solution . while the porous media can be produced from any material compatible with blood , practical considerations dictate that consideration be given first to the use of commercially available materials . the porous medium of this invention may be formed , for example , from any synthetic polymer capable of forming fibers and of serving as a substrate for grafting . preferably , the polymer should be capable of reacting with at least one ethylenically unsaturated monomer under the influence of ionizing radiation without the matrix being significantly or excessively adversely affected by the radiation . suitable polymers for use as the substrate include , but are not limited to , polyolefins , polyesters , polyamides , polysulfones , acrylics , polyacrylonitriles , polyaramides , polyarylene oxides and sulfides , and polymers and copolymers made from halogenated olefins and unsaturated nitriles . examples include , but are not limited to , polyvinylidene fluoride , polyethylene , polypropylene , cellulose acetate , and nylon 6 and 66 . preferred polymers are polyolefins , polyesters , and polyamides . the most preferred polymer is polybutylene terephthalate ( pbt ). surface characteristics of a fiber can be modified by a number of methods , for example , by chemical reaction including wet or dry oxidation , by coating the surface by depositing a polymer thereon , and by grafting reactions which are activated by exposure to an energy source such as heat , a van der graff generator , ultraviolet light , or to various other forms of radiation . the preferred method is a grafting reaction using gamma - radiation , for example , from a cobalt source . radiation grafting , when carried out under appropriate conditions , has the advantage of considerable flexibility in the choice of reactants , surfaces , and in the methods for activating the required reaction . gamma - radiation grafting is particularly preferable because the products are very stable and have undetectably low aqueous extractable levels . furthermore , the ability to prepare synthetic organic fibrous media having a cwst within a desired range is more readily accomplished using a gamma radiation grafting technique . an exemplary radiation grafting technique employs at least one of a variety of monomers each comprising an ethylene or acrylic moiety and a second group , which can be selected from hydrophilic groups ( e . g ., -- cooh , or -- oh ). grafting of the fibrous medium may also be accomplished by compounds containing an ethylenically unsaturated group , such as an acrylic moiety , combined with a hydroxyl group , preferably monomers such as hema or acrylic acid . the compounds containing an ethylenically unsaturated group may be combined with a second monomer such as maa . use of hema as the monomer contributes to a very high cwst . analogues with similar functional characteristics may also be used to modify the surface characteristics of fibers . in a first variation of the devices of this invention , the prp derived from a single unit of about 450 cc of human blood is passed , typically during a flow interval of about 10 to 40 minutes , through a filter comprising grafted fibers , the element of the filter preferably comprising fibers with a surface area in the range of about 0 . 08 to about 1 . 0 square meters , and more preferably about 0 . 1 to about 0 . 7 square meters , with a voids volume in the range of about 50 % to about 89 % ( i . e ., if pbt fiber is used , corresponding to a density of the filter element in the range of about 0 . 69 g / cc to about 0 . 15 g / cc ), and more preferably about 60 % to about 85 % ( for pbt , about 0 . 55 g / cc to about 0 . 21 g / cc ). the filter element is preferably of right cylindrical form with the ratio of diameter to thickness preferably in the range of about 7 : 1 to about 40 : 1 . the range of fiber diameter is preferred to be about 1 . 0 to about 4 μm and is more preferred to be in the range of about 2 to about 3 μm . these parameters can be varied ; for example , the diameter of the filter element could be reduced and the thickness of the filter element increased while retaining the same total quantity of fiber , or the fibers could be larger in diameter while increasing the total quantity of fiber , or the fibers could be packed as opposed to preformed into a cylindrical disc . such variations fall within the purview of this invention . if desired , flow rate of the prp through the filter can be regulated to obtain a total flow period of about 10 to about 40 minutes by selecting the appropriate element diameter , element thickness , fiber diameter , and density , and / or by varying the diameter of tube 12 either upstream or downstream of the filter , or both up and downstream . at these flow rates , leucocyte depletion efficiency in excess of about 99 . 9 % may be achieved and even as high as about 99 . 9995 %. these levels of efficiency result in a pc product with substantially less than about 0 . 1 × 10 6 leucocytes per unit of pc compared with the target of less than about 1 × 10 6 leucocytes per unit . the above - described device and its mode of use provide the advantages set forth below , among other advantages . ( a ) in the blood bank , the filtration step requires no labor input additional to the current practice , and , in the hospital , the need for bedside filtration is completely eliminated . ( b ) the volume of the prp processed is about five or more times that of the pc which is derived from the prp . because the volume processed is larger , loss of pc due to hold up within the filter is only about 1 % compared with a loss about five or more times greater when pc is filtered at bedside . ( c ) compared with hospital practice , filtration within the blood bank is generally under better control , as it is performed in relatively larger numbers by personnel trained to the specific task . ( d ) it is the belief of some researchers that when pc is stored prior to removal of leucocytes , the platelets are damaged during storage as the leucocytes disintegrate , releasing their components , some of which are highly toxic to human tissues . removing the leucocytes within a few hours after collection is believed to greatly reduce damage due to this cause . ( e ) in the process of tapping the donor &# 39 ; s vein , the hypodermic needle cuts a disc of the donor &# 39 ; s skin which is transferred into the collected blood . the alcohol swab applied prior to venipuncture is not adequate to assure sterility of this skin disc . thus , the skin disc may contain one or more varieties of bacteria , the most common being staphylococcus epidermidis , which has been detected in pc along with other organisms . the presence of the skin disc in pc is a suspect source of bacterial growth during storage , and it is fear of such growth which is the principal impetus for the regulation ( in the usa ) which limits the storage life of platelets to five days . removal of the skin disc by filtration at an early stage of processing is , for this reason , an important advantage as it may permit the five day regulation to be relaxed . ( f ) compared with a bedside filtration method of &# 39 ; 548 , improved recovery of platelets is obtained , i . e ., recovery in excess of 98 % to 99 % compared with about 90 to 95 % typically recovered in bedside filtration . in a second variation of this invention , the interposed filter 14 is preferably made with smaller fiber surface area , smaller filter element flow area , higher filter element density , and reduced voids volume in relation to the first variation . the total quantity of fiber used is also reduced such that a preferred range for the fiber surface area of the filter element is about 0 . 04 to about 0 . 3 m 2 and a more preferred range is about 0 . 06 to about 0 . 20 m 2 . a preferred range for the filter element flow area is about 3 to about 8 cm 2 , and a more preferred range is about 4 to about 6 cm 2 . a preferred range for the relative voids volume is about 71 % to about 83 % ( corresponding for pbt fibers to a density of about 0 . 23 to about 0 . 40 g / cc ), and a more preferred range is from about 73 % to about 80 % ( about 0 . 27 to about 0 . 37 g / cc ). a preferred range for the cwst of the fiber is about 70 to about 115 dynes / cm , a more preferred range is about 90 to about 100 dynes / cm , and a still more preferred range is about 93 to about 97 dynes / cm . because of its very small size , a preferred device in accordance with the second variation of the invention retains internally only 0 . 5 to 1 cc of prp , representing less than a 0 . 5 % loss of platelets . this second variation may also comprise a porous medium wherein the upstream portion of the medium is of a higher density than the downstream portion . for example , the porous medium may comprise a higher density upstream layer for blocking the passage of red blood cells and a lower density downstream layer for the depletion of leucocytes . filters made in accordance with this second variation and which are interposed between the blood collection bag and prp bag will generally remove about 85 to 99 % or more of the incident leucocytes , a removal rate that is not sufficient to consistently achieve a residual leucocyte count of less than 10 7 leucocytes per 50 ml of pc ( see table ii ). a principal function of this device , however , is to act as an automatic &# 34 ; valve &# 34 ; during the decantation process by instantly stopping the flow of prp at the moment that red cells contact the filter surface . the mechanism of this valve - like action is not well understood , but it may reflect aggregation of the red cells as they reach the filter surface , forming a barrier which prevents or blocks further flow of prp through the filter element . aggregation of red cells on contact with the filter surface appears to be related to the cwst and / or to the surface characteristics of the fibers which are generated by the herein described procedure for modifying the fibers . this theory for the proposed mechanism is supported by the existence of filters capable of highly efficient leucocyte depletion of human red blood cell suspensions and which have pore sizes as small as 0 . 5 μm , through which red cells pass freely and completely with no clogging , with applied pressure of the same magnitude as that used in the present invention . on the other hand , filters of the present invention , which typically have pore diameters larger than about 0 . 5 μm , abruptly stop the flow of red blood cells when the filter is contacted by the red cells . this suggests that the filter &# 39 ; s valve - like action is not related to or caused by pore size or by a filtration mechanism . the mechanism of this valve - like action is not well understood , but it may reflect zeta potential - related aggregation of the red cells as they reach the filter surface , forming a barrier which prevents or blocks further flow of prp through the filter element . the advantages to be gained by the use of this device include the following : ( a ) the collected prp , and the pc derived therefrom , are substantially free of red cells . ( b ) the operator needs only to start the flow of prp , which will continue to flow into the first satellite bag until red cells contact the filter surface , at which point flow stops . this eliminates the need for a skilled operator to estimate when to stop flow . the prp so obtained has the faintly yellow color of normal prp and , for practical purposes , may be considered to be free of red cells . the pc derived from the prp has the characteristic light yellow color of pc and , for practical purposes , may be considered to be essentially free of red cells . ( c ) the volume of prp recovered from the blood collection bag during the plasma extraction operation is increased by about 2 % to about 3 % when compared with very competent manual operation and probably by about 2 % to about 5 % compared with average blood bank practice . ( d ) labor input is reduced , as monitoring of the interface during decantation is not required . ( e ) freshly donated blood contains platelets varying in age from newly formed to nine days or more ( platelet half - life in vivo is about nine days ). newly formed platelets are larger and are generally believed to be more active . because the younger platelets are larger , they tend to sediment faster during centrifugation and , consequently , are present in larger numbers in the prp nearest to the red cell interface . measurements have shown that the concentration of platelets in the 10 % of the prp volume nearest the interface is about twice that in the uppermost 10 % of prp . taking this into account , the total number of platelets recovered is increased by about 4 to 10 %. ______________________________________ incremental number of platelets , % ______________________________________due to increased volume 2 to 5of prpdue to the higher 2 to 5concentration ofplatelets in theincremental volumeof prptotal 4 to 10 % ______________________________________ ( f ) the larger proportion of younger platelets in the pc administered to the patient means that their life within the patient after administration will be longer and that the platelets will be more active , compared with current blood bank practice . ( g ) the yield of plasma , a component of value comparable with that of prc and pc , is also increased by about 2 to about 5 %. ( h ) insofar as the plasma yield is increased , the plasma content of the prc is decreased . this is advantageous because the mhc ( major histocompatibility complex ) contained in the plasma is responsible for the occurrence of urticaria ( hives ) in a proportion of transfusion recipients transfused with prc . in a third variation of this invention , the fiber is surface modified in the same manner as for the preceding versions , but the fiber surface area of the element is increased while , at the same time , the density of the filter element is somewhat reduced . in this way , the automatic blockage of flow on contact by red cells is combined with higher efficiency of leucocyte depletion . a preferred range of fiber surface area for the third variation of the invention is from about 0 . 3 to about 2 . 0 m 2 , and a more preferred range is from about 0 . 35 to about 0 . 6 m 2 . the upper limits of fiber surface area reflect the desire to accomplish the filtration in a relatively short time period , and may be increased if longer filtration times are acceptable . a preferred voids volume of a filter for a filter element is in the range of about 71 % to about 83 % ( i . e ., if pbt fiber is used , corresponding to a density of the filter element in the range of about 0 . 24 g / cc to about 0 . 40 g / cc ), and more preferably about 75 % to about 80 % ( for pbt , about 0 . 28 g / cc to about 0 . 35 g / cc ). a preferred filter element flow area is from about 2 . 5 to about 10 cm 2 , and a more preferred area is from about 3 to about 6 cm 2 . leucocyte depletion efficiencies in excess of about 99 . 9 to about 99 . 99 %, which corresponds to an average residual leucocyte content per unit of less than about 0 . 005 × 10 7 , can be obtained . for all three of the above described variations of the porous medium for use with prp , a preferred range for the cwst of the fiber is preferably above about 70 dynes / cm , typically about 70 to 115 dynes / cm ; a more preferred range is 90 to 100 dynes / cm , and a still more preferred range is 93 to 97 dynes / cm . a preferred range for the zeta potential ( at the ph of plasma ( 7 . 3 )) is about - 3 to about - 30 millivolts , a more preferred range is about - 7 to about - 20 millivolts , and a still more preferred range is about - 10 to about - 14 millivolts . housings for the leucocyte depletion media of the invention can be fabricated from any suitably impervious material , including an impervious thermoplastic material . for example , the housing may preferably be fabricated by injection molding from a transparent or translucent polymer , such as an acrylic , polystyrene , or polycarbonate resin . not only is such a housing easily and economically fabricated , but it also allows observation of the passage of the fluid through the housing . the housing into which the porous medium is sealed or interference fit is designed to achieve convenience of use , rapid priming , and efficient air clearance . the blood collection and processing assembly 10 , with one or more satellite bags attached or connected via a conduit , may be used integrally to separate components from whole blood . during the centrifugation step in which the red cells are concentrated at the bottom of collection bag 11 , forces of up to about 5000 times gravity ( 5000 g ) or more may be generated . therefore , collection bag 11 is preferably flexible , as are the other bags , allowing them to settle to the bottom and against the walls of a centrifuge bucket , so that the bags themselves are subject to little or no stress . a ) blood product : anti - coagulated whole blood ( awb ); packed red cells obtained from awb ; platelet - rich plasma ( prp ) obtained from awb ; platelet concentrate ( pc ) obtained from awb or prp ; plasma obtained from awb or prp ; red cells separated from plasma and resuspended in physiological fluid ; and platelets separated from plasma and resuspended in physiological fluid . as used herein , blood component or product refers to the components described above , and to similar blood products obtained by other means and with similar properties . in accordance with the invention , each of these blood products is processed in the manner described herein . b ) unit of whole blood : blood banks in the united states commonly draw about 450 milliliters ( ml ) of blood from the donor into a bag which contains an anticoagulant to prevent the blood from clotting . however , the amount drawn differs from patient to patient and donation to donation . herein the quantity drawn during such a donation is defined as a unit of whole blood . c ) unit of packed red cells ( prc ), platelet - rich plasma ( prp ), or platelet concentrate ( pc ): as used herein , a &# 34 ; unit &# 34 ; is defined by the united states &# 39 ; practice , and a unit of prc , prp , pc , or of red cells or platelets in physiological fluid or plasma , is the quantity derived from one unit of whole blood . typically , the volume of a unit varies . for example , the volume of a unit of prc varies considerably dependent on the hematocrit ( percent by volume of red cells ) of the drawn whole blood , which is usually in the range of about 37 % to about 54 %. the concomitant hematocrit of prc , which varies over the range from about 50 to over 80 %, depends in part on whether the yield of one or another blood product is to be minimized . most prc units are in the range of about 170 to about 350 ml , but variation below and above these figures is not uncommon . d ) porous medium : refers to the porous medium through which one or more blood components pass . the platelet or prp porous medium refers generically to any one of the media which deplete leucocytes from the non - prc blood components , i . e ., from prp or from pc . the red cell barrier medium blocks the passage of red cells and depletes leucocytes from prp to a greater or lesser degree while allowing the passage of platelets . the porous medium for use with prp may be formed from any natural or synthetic fiber or other porous material compatible with blood . preferably , the cwst and zeta potential of the porous medium are within certain ranges , as disclosed above and as dictated by its intended use . for example , the cwst of a prp porous medium is typically above about 70 dynes / cm . the porous media according to the invention may be connected to a conduit interposed between the containers , and may be positioned in a housing which in turn can be connected to the conduit . as used herein , filter assembly refers to the porous medium positioned in a suitable housing . preferably , the porous medium forms an interference fit at its edges when assembled into the housing . the porous medium may be configured as a flat sheet , a corrugated sheet , a web , or a membrane , although it is not intended that the invention should be limited thereby . e ) voids volume is the total volume of all of the pores within a porous medium . voids volume is expressed hereinafter as a percentage of the apparent volume of the porous medium . f ) conversion of density when using fibers other than pbt : in the preceding exposition the term density has been used , and the density values quoted for the filter element have been based on the use of pbt fibers . other fibers which differ in density from the pbt may be used , as noted above , providing that their surfaces have , or have been modified to have , the characteristics noted above , e . g ., a cwst of greater than 70 dynes / cm . in accordance with the invention , to use an alternate fiber of different density , the density of an element made using an alternate fiber may be calculated as follows : denoting v as a percentage of the voids volume relative to the apparent volume of the pbt element [ i . e ., v =( volume of voids / volume of element )× 100 ], the objective is to calculate the element density of an alternate fiber element which will have a relative voids volume percentage equal to v . if f is the density of the alternate fiber and 1 . 38 g / cc is taken as the density of pbt fiber , and m 1 is the element density of the pbt element and m 2 is the density required for an element with equivalent performance , then voids volume v of the pbt fiber element is and the density required for the element made using the alternate fiber is the more preferred fiber diameter range for the practice of this invention is about 2 to 3 μm , the diameter being defined in terms of surface area , as described in u . s . pat . no . 4 , 880 , 548 . this range is preferred because much above this range , the dimensions of the elements and consequently the liquid hold - up volumes of the filters become significantly larger ; below this range , the filter elements become relatively less coherent and are more easily compressed . for example , an element made using less than 2 μm polypropylene fibers would be compressed by the pressure developed by the plasma extractor , which can be as high as 300 mm of hg . pore diameters of filter elements in accordance with the invention can be determined using the modified osu f2 method as described in u . s . pat . no . 4 , 925 , 572 . g ) in accordance with the invention , a useful technique for the measurement of fiber surface area , for example by nitrogen gas adsorption , is that developed by brunauer , emmet , and teller in the 1930 &# 39 ; s , often referred to as the &# 34 ; bet &# 34 ; measurement . using pbt as an example , the surface area of melt blown webs can be used to calculate average fiber diameter : total volume of fiber in 1 gram = 1 / 1 . 38 cc ( where 1 . 38 = fiber density of pbt , g / cc ) ## equ1 ## dividing ( 1 ) by ( 2 ), ## equ2 ## where l = total length in cm of 1 gram of fiber , if the units of d are micrometers , the units of a f become m 2 / g ( square meters / gram ), which will be used hereinafter . for fibers other than pbt , substitute the density for 1 . 38 . h ) general procedure for measuring zeta potential : zeta potential was measured using a sample cut from a 1 / 2 inch thick stack of webs . the zeta potential was measured by placing the sample in an acrylic filter holder which held the sample snugly between two platinum wire screens 100 × 100 mesh ( i . e ., 100 wires in each direction per inch ). the meshes were connected , using copper wire , to the terminals of a triplett corporation model 3360 volt - ohm meter , the mesh on the upstream side of the sample being connected to the positive terminal of the meter . a ph - buffered solution was flowed through the sample using a differential pressure of 45 inches of water column across the filter holder and the effluent was collected . for measurements at ph 7 , a buffered solution was made by adding 6 ml ph 7 buffer ( fisher scientific co . catalog number sb108 - 500 ) and 5 ml ph 7 . 4 buffer ( fisher scientific co . catalog number sb110 - 500 ) to 1 liter pyrogen - free deionized water . for measurements at ph 9 , a buffered solution was made by adding 6 ml ph 9 buffer ( fisher scientific co . catalog number sb114 - 500 ) and 2 ml ph 10 buffer ( fisher scientific co . catalog number sb116 - 500 ) to 1 liter pyrogen - free deionized water . the electrical potential across the filter holder was measured during flow ( it required about 30 seconds of flow for the potential to stabilize ) and was corrected for cell polarization by subtracting from it the electrical potential measured when flow was stopped . during the period of flow the ph of the liquid was measured using a cole - parmer model j - 5994 - 10 ph meter fitted with an in - line model j - 5993 - 90 ph probe . the conductivity of the liquid was measured using a cole - parmer model j - 1481 - 60 conductivity meter fitted with a model j - 1481 - 66 conductivity flow cell . then the polarity of the volt meter was reversed , and the effluent was flowed backwards through the filter holder using a differential pressure of 45 inches of water column . as in the first instance the electrical potential measured during flow was corrected for cell polarization by subtracting from it the electrical potential measured after flow was stopped . the average of the two corrected potentials was taken as the streaming potential . the zeta potential of the medium was derived from the streaming potential using the following relationship ( j . t . davis et al ., interfacial phenomena , academic press , new york , 1963 ): ## equ3 ## where η is the viscosity of the flowing solution , d is its dielectric constant , λ is its conductivity , e s is the streaming potential and p is the pressure drop across the sample during the period of flow . in these tests the quantity 4 πη / dp was equal to 0 . 800 . each of the examples was run using the following basic procedure to process and test a bag of donated blood . the blood collection set was constituted as shown in fig1 . bag 11 , into which anticoagulant had been placed , was used to collect one unit of about 450 cc of blood from a human volunteer . bag 11 along with its two satellite bags was then centrifuged for 5 minutes at 2280 × gravity , causing the red cells to sediment into the lower parts of the bag and leave a transparent , yellowish layer of red cell - free plasma in the upper part of the bag . this bag was then transferred , with care not to disturb its contents , to a plasma extractor . with tube 12 clamped adjacent to bag 11 to prevent flow , tube 12 was cut and the test filter was inserted at position 14 in fig2 . with the plasma extractor applying sufficient force to the bag to generate a pressure of about 200 to 300 millimeters of mercury within the bag , the clamp on tube 12 was removed , allowing the supernatant liquid to flow through the filter into bag 13 which had been placed on a weight scale . one of several skilled operators was instructed to signal when , in normal blood bank practice , flow would have been manually shut off . for examples 1 and 2 , which were in accordance with the first variation of this invention , tube 12 was at the signal promptly shut - off , the weight of prp collected was recorded , and the contents of the bag analyzed , with results recorded in table i . for examples 3 - 8 and 9 - 10 , the weight of the prp bag 13 was recorded at the signal , i . e ., the precise moment when flow would in normal blood bank practice have been shut off , while flow was allowed to continue until the red cell layer reached filter 14 , at which time flow spontaneously and abruptly stopped , and the weight of prp collected was recorded . the results for examples 3 - 8 are shown in table ii , and for examples 9 and 10 in table iii . in each of the ten examples , the resulting prp was visually free of red cells , and weights of the prp were converted to volume by dividing by the density of plasma ( 1 . 04 g / cc ). the data on residual leucocyte content of the pc derived from the filtered prp are reported in tables ii and iii as multiples of 10 7 ( i . e ., × 10 7 ), which can be conveniently compared with a target criterion of fewer than about 1 × 10 7 leucocytes per unit , which is a level believed adequate to significantly reduce alloimmunization in patients receiving platelet transfusions . the widely used melt blowing process for making fibrous plastic webs is a convenient , economical , and effective means for manufacturing fibrous webs with fiber diameter in the 1 - 4 μm range . it is characteristic of this process that the quality of melt blown webs is optimal when the web weight is maintained in a preferred range of about 0 . 0005 to about 0 . 01 g / cm 2 , and more preferably between about 0 . 0005 and about 0 . 007 g / cm 2 . for this reason , the webs used to form the examples of this invention were , wherever necessary , formed by laying up two or more layers of web of weight about 0 . 006 g / cm 2 , and then hot compressing these to form an integral filter element . devices were prepared in the manner of the first variation of this invention . the filter elements of these devices were preformed from 2 . 6 μm average diameter pbt fibers , which had been surface modified in the manner as described above and as taught in u . s . pat . no . 4 , 880 , 548 using a mixture of hydroxyethyl methacrylate and methacrylic acid in a monomer ratio of 0 . 35 : 1 to obtain a cwst of 95 dynes / cm and a zeta potential of - 11 . 4 millivolts . filter element effective diameter was 4 . 74 cm , presenting a filter area of 17 . 6 cm 2 , thickness was 0 . 15 cm , voids volume was 83 % ( density = 0 . 23 g / cc ), and fiber surface area was 0 . 69 m 2 . the volume of prp held up within the filter housing was 2 . 5 cc , representing a loss of prp due to hold - up of about 1 %. the results , obtained using the operating procedure described earlier in this section for the first variation , are shown in table i . table i______________________________________leucocyte depletion efficiency of the first variation leucocyte volume content of leucocyte of prp pc after removalexample passed , filtration efficiency ,** number cc ( per unit )* % ______________________________________1 237 & lt ;. 006 × 10 . sup . 7 & gt ; 99 . 9 % 2 206 & lt ;. 006 × 10 . sup . 7 & gt ; 99 . 9 % ______________________________________ * total leucocyte count in the pc after centrifuging the filteredprp to obtain the pc . ** assumes that the leucocyte content of the prp prior tofiltration conformed to an average value of 5 × 10 . sup . 7 per______________________________________unit . devices were prepared in the manner of the second (&# 34 ; automatic valve &# 34 ;) variation of this invention . the filter elements of these devices were preformed from 2 . 6 μm average diameter pbt fibers , which had been surface modified in the manner as described above and as taught in u . s . pat . no . 4 , 880 , 548 using hydroxyethyl methacrylate and methacrylic acid in a monomer ratio of 0 . 35 : 1 to obtain a cwst of 95 dynes / cm and a zeta potential of - 11 . 4 millivolts . the filter element &# 39 ; s effective diameter was 2 . 31 cm , presenting a filter area of 4 . 2 cm 2 , thickness was 0 . 051 cm , voids volume was 75 % ( density , 0 . 34 g / cc ), and fiber surface area was 0 . 08 m 2 . the volume of prp held up within the filter housing was & lt ; 0 . 4 cc , representing a loss of prp due to hold - up of less than 0 . 2 %. in each test , flow stopped abruptly as red cells reached the upstream surface of the filter element , and there was no visible evidence of red cells or hemoglobin downstream . the results obtained , using the operating procedure described earlier in this section for the second variation , are shown in table ii . table ii______________________________________ 5 leuco - 3 cyte volume content 2 of prp after estimated obtained 4 filtrat - volume / prp using the incre - ion ( per1 using normal procedure mental unit ) example blood bank of inven - volume , of pc * × number practice , ml tion , ml percent 10 . sup . 7______________________________________3 175 . 2 178 . 8 2 . 0 1 . 04 212 . 9 218 . 8 2 . 7 1 . 75 221 . 1 225 . 7 2 . 0 0 . 56 185 . 9 191 . 4 2 . 9 0 . 27 257 . 2 263 . 2 2 . 3 & lt ; 0 . 18 196 . 6 200 . 7 2 . 1 0 . 1______________________________________ * total leucocyte count in the pc after centrifuging the filtered prp to obtain pc . devices were prepared in the manner of the third variation of this invention , i . e ., the combination of an automatic shut - off valve and a high efficiency filter , both comprised in a single filter . the filter elements of these devices were preformed from 2 . 6 μm average diameter pbt fibers , which had been surface modified in the manner as described above and as taught in u . s . pat . no . 4 , 880 , 548 using a mixture of hydroxyethyl methacrylate and methacrylic acid in a monomer ratio of 0 . 35 : 1 to obtain a cwst of 95 dynes / cm and a zeta potential of - 11 . 4 millivolts at the ph of plasma ( 7 . 3 ). the filter element effective diameter was 2 . 31 cm presenting a filter area of 4 . 2 cm 2 thickness was 0 . 305 cm , density was 0 . 31 g / cc ( voids volume = 77 . 5 %), and fiber surface area was 0 . 46 m 2 . the volume of prp held up within the filter housing was 1 . 3 cc , representing a loss of prp due to hold up within the filter of about 0 . 5 %. in each case , flow stopped abruptly as red cells reached the upstream surface of the filter element , and there was no visible evidence of red cells or hemoglobin downstream . the results obtained , using the operating procedure described earlier in this section for the third variation , are shown in table iii . table iii__________________________________________________________________________incremental volume and leucocvre depletionefficiency of the third variation volume of prp leucocyneestimated obtained contentvolume / prp using the incre - afterusing normal procedure mencal filtration leucocyteexampleblood bank of inven - volume , ( per unit ) removalnumberpractice , ml tion , ml % of pc * × 10 . sup . 7 efficiency ** __________________________________________________________________________ 9 251 256 2 & lt ;. 004 & gt ; 99 . 9 % 10 212 216 1 . 9 . 005 & gt ; 99 . 9 % __________________________________________________________________________ * total leucocyte count in the pc after centrifuging the filtered prp toobtain pc . ** assumes that the leucocyte content of the prp prior to filtrationconformedto an average value of 5 × 10 . sup . 7 per unit . __________________________________________________________________________ while the invention has been described in some detail by way of illustration and example , it should be understood that the invention is susceptible to various modifications and alternative forms , and is not restricted to the specific embodiments set forth in the examples . it should also be understood that these examples are not intended to limit the invention but , on the contrary , the intention is to cover all modifications , equivalents , and alternatives falling within the spirit and scope of the invention .
a method of preparing platelets for long term storage comprises the steps of obtaining whole blood from a human , preparing platelet rich plasma from the whole blood and then passing the platelet rich plasma through a filter under conditions sufficient to remove substantially all leucocytes from the plasma , with steps and occurring in an integrally attached blood bag system within a few hours of step .