hereinafter , the technical solutions in the examples of the present invention will be described clearly and completely in conjunction with examples of the present invention . it is apparent that the described examples are merely a part of the examples of the present invention rather than all . based on the examples of the present invention , all other examples obtained by a person skilled in the art without creative work are within the scope of the present invention . to further understand the present invention , detailed descriptions are provided in combination with the following examples . example 1 : an acne - removing chinese medicine composition of the present invention 1 . mangosteen pericarp , thyme and fructus aurantii were crushed respectively , passed through a 60 - mesh sieve , and mixed in a ratio of 80 g mangosteen pericarp , 20 g thyme and 20 g fructus aurantii ; 2 . reflux extraction was performed for 2 . 5 h by using 95 % ethanol - water solution in a amount of 12 folds by weight of the raw materials ; 3 . the above extraction solution was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 72 g concentrated solution with a specific gravity of 1 . 08 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 80 %, added to the concentrated solution at a volume ratio of 6 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 80 ° c . for 2 h , and refrigerated overnight at 4 ° c . for 18 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 40 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 1bv , the amount of 40 % ethanol - water solution used is 1bv , and the amount of 80 % ethanol - water solution used is 4bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 06 , followed by spay drying to obtain 5 . 04 g . example 2 : an acne - removing chinese medicine composition of the present invention 1 . mangosteen pericarp , thyme and fructus aurantii were crushed respectively , passed through a 60 - mesh sieve , and mixed in a ratio of 60 g mangosteen pericarp , 10 g thyme and 10 g fructus aurantii ; 2 . reflux extraction was performed for 1 h by using 60 % ethanol - water solution in an amount of 8 folds by weight of the raw materials ; 3 . the above extract was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 106 g concentrated solution with a specific gravity of 1 . 08 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 60 %, added to the concentrated solution at a volume ratio of 4 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 50 ° c . for 0 . 5 h , and refrigerated overnight at 0 ° c . for 12 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 76 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 2bv , the amount of 40 % ethanol - water solution used is 2bv , and the amount of 80 % ethanol - water solution used is 2bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 06 , followed by spay drying to obtain 3 . 2 g . example 3 : an acne - removing chinese medicine composition of the present invention 1 . mangosteen pericarp , thyme and fructus aurantii were crushed respectively , passed through a 60 - mesh sieve , and mixed in a ratio of 80 g mangosteen pericarp , 10 g thyme and 10 g fructus aurantii ; 2 . reflux extraction was performed for 2 h by using 75 % ethanol - water solution in a amount of 10 folds by weight of the raw materials ; 3 . the above extract was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 106 g concentrated solution with a specific gravity of 1 . 06 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 70 %, added to the concentrated solution at a volume ratio of 5 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 60 ° c . for 1 h , and refrigerated overnight at 2 ° c . for 15 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 76 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 1bv , the amount of 40 % ethanol - water solution used is 2bv , and the amount of 80 % ethanol - water solution used is 3bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 06 , followed by spay drying to obtain 4 . 4 g . 1 . 100 g mangosteen pericarp was crushed , and passed through a 60 - mesh sieve ; 2 . reflux extraction was performed for 2 h by using 75 % ethanol - water solution in a amount of 10 folds by weight of the raw materials ; 3 . the above extract was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 62 g concentrated solution with a specific gravity of 1 . 06 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 70 %, added to the concentrated solution at a volume ratio of 5 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 60 ° c . for 1 . 5 h , and refrigerated overnight at 2 ° c . for 15 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 54 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 1bv , the amount of 40 % ethanol - water solution used is 2bv , and the amount of 80 % ethanol - water solution used is 3bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 08 , followed by spay drying to obtain 4 . 0 . 1 . 100 g thyme was crushed , and passed through a 60 - mesh sieve ; 2 . reflux extraction was performed for 2 h by using 75 % ethanol - water solution in a amount of 10 folds by weight of the raw materials ; 3 . the above extract was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 52 g concentrated solution with a specific gravity of 1 . 06 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 70 %, which was added to the concentrated solution at a volume ratio of 5 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 60 ° c . for 1 . 5 h , and refrigerated overnight at 2 ° c . for 15 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 38 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 1bv , the amount of 40 % ethanol - water solution used is 2bv , and the amount of 80 % ethanol - water solution used is 3bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 06 , followed by spay drying to obtain 1 . 2 g . 1 . 100 g fructus aurantii was crushed , and passed through a 60 - mesh sieve ; 2 . reflux extraction was performed for 2 h by using 75 % ethanol - water solution in a amount of 10 folds by weight of the raw materials ; 3 . the above extract was filtered through 100 - mesh filter cloth to remove the residue , concentrated under reduced pressure ( temperature 60 ° c .- 80 ° c ., vacuum degree ≧ 0 . 08 mpa , the same below ) to obtain 70 g concentrated solution with a specific gravity of 1 . 06 ; 4 . recycled ethanol - water solution was adjusted to a concentration of 70 %, added to the concentrated solution at a volume ratio of 5 : 1 ( ethanol - water solution : concentrated solution ); stirred and dissolved at 60 ° c . for 1 . 5 h , and refrigerated overnight at 2 ° c . for 15 h ; 5 . the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 58 g product with a specific gravity of 1 . 08 ; 6 . the concentrated solution was adsorbed by polyamide resins and eluted with water , 40 % ethanol - water solution , 80 % ethanol - water solution respectively , wherein the amount of water used is 1bv , the amount of 40 % ethanol - water solution used is 2bv , and the amount of 80 % ethanol - water solution used is 3bv ; 7 . a fragment eluted by 80 % ethanol - water solution was collected and concentrated under reduced pressure to a specific gravity of 1 . 05 , followed by spay drying to obtain 1 . 8 g . 1 . 2 chinese medicine composition samples : d1 , d2 , d3 , d4 , d5 and d6 , stored at 4 ° c . for use , wherein samples d1 to d6 correspond to the products prepared by examples 1 , 2 and 3 , and comparative examples 1 , 2 and 3 , respectively . 1 . 3 culture medium : propionibacterium acnes culture medium ( ph was adjusted to 6 . 6 to 7 . 0 ). agar was added in 15 g per liter to the solid culture medium . 0 . 1 ml frozen deposit bacteria suspension was added to 5 ml propionibacterium acnes culture medium and cultured at 37 ° c . under anaerobic condition for 2 days , thus the bacteria suspension for the experiment was obtained . samples d1 , d2 , d3 , d4 , d5 and d6 were diluted in saline to the test concentration of 0 . 1 %, followed by gradient dilution , respectively . in the first test , the gradient concentrations were 100 % ( original sample solution ), 50 %, 25 %, 12 . 5 %, 6 . 25 %, 3 . 125 %, 1 . 56 % and 0 . 78 %, respectively . in the second test , the gradient diluted concentrations for second test were 10 %, 8 %, 6 %, 4 % and 2 %, respectively . the bacteria suspension was diluted in propionibacterium acnes culture medium to a final concentration of 10 6 cfu / ml . 100 μl bacteria suspension and 100 μl sample solution were added to the well . the negative control without adding bacteria and the normal growth control without adding test solution were set at the same time . each sample was performed in triplicate and the average was taken . results were observed after anaerobic incubation at 37 ° c . for 48 h . the presence of turbidity was judged by naked eye and data were read out directly . the prerequisites for result judgement were : the growth control is well , there is no bacteria and the growth is clear for the blank control , and the growth of bacteria in other wells was inhibited with the increasing gradient concentrations of the drugs . it can be found from the results in table 1 that the solutions from the three examples with a concentration of 0 . 1 % all had good growth inhibition on propionibacterium acnes when they were diluted to 12 . 5 % or higher concentrations . however , in comparative samples , a desirable effect can be achieved when the concentration of the diluted solutions is 25 % or higher for d4 and the concentration is 50 % or higher for d5 and d6 . it can be found from the results in table 2 , after verification , the bacteriostasis effect can be achieved by d1 and d3 at a concentration of 8 %, and by d2 at a concentration of 10 %. it can be known from the above results that minimal addition of the chinese medicine composition product of the present invention in about 0 . 01 % can achieve basically the inhibition effect on propionibacterium acnes . also , the chinese medicine composition of the present invention has a stronger bacteriostasis effect than the medicinal material used solely . 1 . 1 experimental animals : balb / c mice ( female , 8 - week old , body weight 20 g - 25 g ) were purchased from shanghai slac laboratory animal co . ltd . ( license no . scxk ( hu ) 2007 - 9005 ). 1 . 2 main experimental reagents : 2 , 4 - dinitrochlorobenzene ( dncb , analytically pure ) was purchased from shanghai shunqiang biotechnology co . ltd . ; acetone ( analytically pure ) was purchased from sinopharm chemical reagent co . ltd . 2 . chronic eczematous dermatitis mouse model induced by dncb and drug therapy on the first day of experiment ( d0 ), all mice were shaved on the back of an area about 2 cm × 2 cm . 100 μl of 7 % dncb solution in acetone was taken by pipette and applied to the shaved area of the back of the mice to induce hypersensitivity . from d5 , 20 μl of 0 . 1 % dncb solution was applied to the inner side of the right ear of the mice to challenge and induce chronic dermatitis , once in every three days , total 5 times . to evaluate the anti - dermatitis effects of d1 - d6 , after the first challenge , different concentrations of diluted solution or solvent of d1 - d6 were applied to the sensitized area of the right ear and the back of the mice , twice per day . skin lesions of the sensitized area of the back of the mice in the dermatitis model group and drug treatment group were observed 48 h after each challenge . all mice were sacrificed 48 h after the last challenge . left and right auricle pieces ( diameter about 8 mm ) were removed by a puncher and their mass were measured on an analytical balance . the mass difference between the two auricles , which reflects the degree of ear swelling , was calculated . 3 . experimental results 3 . 1 primary evaluation results of anti - dermatitis effect of d1 - d6 mice were divided into 5 groups at random and each group has 4 mice used in the evaluation experiment for each component . these 5 groups are : normal control group , solvent control group , 5 %, 10 % and 15 % d1 - d6 diluted solution treatment groups , respectively . for the normal control group , only acetone was applied to the hair - removed - area on the back and right ear . for the solvent control group , dncb solution in acetone was applied to induce dermatitis on the hair - removed - area on the back and right ear , and after the first challenge the solvents for the test component ( 0 . 1 % solution in water for d1 - d6 ) were applied . for the 5 %, 10 % and 15 % d1 - d6 diluted solution treatment groups , dncb solution in acetone was applied to the hair - removed - area on the back and right ear to induce dermatitis , followed by application of test components after first challenge , wherein the concentrations thereof are 5 %, 10 % and 15 %, respectively . skin lesions of the sensitized area of the back of the mice in all experimental groups were observed 48 h after each challenge , and photos were taken . the results indicated that components d5 and d6 basically failed to show the pharmacological effect of relieving dermatitis in the dose range of 5 %- 15 %, while components d1 to d4 have certain pharmacological effect of relieving dermatitis at the dose of 15 %. the results are shown in table 3 . it can be known from the data in table 3 , 0 . 1 % solutions of d1 - d4 all have an inflammation - relieving effect at different concentrations , but the effect was not remarkable at the concentration 5 %, slightly improved at the concentration 10 % and remarkable at the concentration 15 %. in vertical comparison , d1 , d2 and d3 have similar effect overall and are slightly better than d4 . while d5 and d6 had not received remarkable relief of the inflammation on the back , thus no comparison was made . it can be found from the results of bacteriostasis test , the minimum application concentration of d3 can be as low as 8 % while the minimum concentration of d4 is higher than 12 . 5 %. it can be found from the ear swelling data in mouse inflammation model , d3 and d4 solutions have similar effect on inflammation relieving at the concentration 5 %, while at the concentrations 10 % and 15 %, the data of d3 is much smaller than that of d4 with a difference of higher than 20 %, showing d3 has a much better anti - inflammation effect than d4 at the same concentration . these results indicated that mangosteen pericarp , thyme and fructus aurantii have a synergic effect on bacteriostasis and inflammation relieving . 0 . 1 % of the acne - removing chinese medicine composition in example 1 of the present invention was added to general basal cream and applied to lesions of the subjects . apparent redness and swellings were relieved in 0 . 5 h - 1 h ; no uncomfortable feelings from acne , such as swelling and pricking pain , in 4 h - 6 h ; and all the wounds scabbed within 24 h . after a long term application , those subjects who suffered from acne for a long time , had a significantly decreased distribution density of acne on their faces , and the inflammations were more slight than normal population when acne occurred . the acne - removing chinese medicine compositions of example 2 and example 3 have comparable effect as example 1 in clinic .
disclosed are an acne - removing traditional chinese medicine composition and a preparation method thereof , wherein the traditional chinese medicine composition is made from garcinia mangostana l pericarp , thyme and fructus aurantii through steps of ethanol extraction and macroporous resin , and collecting the part eluted by 60 %- 80 % ethanol . the three medicinal materials of the composition have a synergic anti - inflammation effect .