the clean glass utensil is dried then keeped in oven at 300 ° c . for 3 hours ( the heat source is removed till the bacteria are killed ); 1 . 5 kg paclitaxel available in market is weighed and placed in the glass utensil mentioned above , propylene glycol is added dropwise till paclitaxel is completely dissolved to make a paclitaxel solution of 10 mol / l ; 2 . 0 kg sodium alginate available in market is weighed and placed in the glass utensil , normal saline is added while stirring till sodium alginate is completely dissolved ; the mixed solution is extracted by aseptic syringe and added into the calcium chloride solution under the high - voltage electrostatic droplet device . the sodium alginate microsphere containing paclitaxel settles at the bottom of the glassware with diameter of 200 ˜ 550 μm . the upper solution is decanted and the lower microgel particles are put into the oven and keeped in a sealed condition . the diameters of dry - microspheres are 100 ˜ 350 μm . prior to use , the dryp - microspheres should be rehydrated with normal saline for a couple of minutes . alternatively , the wet - microspheres can be used directly after the decanter of the upper solution and washed twice . as for patients with myometrial gland , by adopting intervention radiotherapy or intervention ultrasound , insert the duct into feeding artery of target organ to conduct arteriography . paclitaxel - containing sodium alginate microsphere with diameter ranging from 200 - 550 μm is selected according to the impression of arteriography . try to use micro - duct to carry out ultraselective embolus with aseptic operation . extract cacl 2 solution from the bottle using injector and add in equal volume normal saline to rinse paclitaxel - containing sodium alginate microsphere ( wet ball ) for 3 times , or extract the described cacl 2 solution from the bottle and add in equal volume normal saline , pour normal saline and microsphere into an aseptic bowl . rinse the microsphere using 50 - 60 ml normal saline once and discard the rinse solution , and then add in appropriate amount or diluted contrast medium to mix up evenly ( leaving microsphere thoroughly floating in the contrast medium ), and infuse it into the foci slowly or slowly in multiple times depending on concrete conditions by duct under fluoroscopy ( prohibit to overdose of embolism .) till flow rate of contrast medium is obviously reduced , i . e . embolism is finished . arteriography is conducted again to judge the effect of embolism . the clean glass utensil is dried then keeped in oven at 300 ° c . for 3 hours ( the heat source is removed till the bacteria are killed ); 2 kg paclitaxel available in market is weighed and placed in the glass utensil mentioned above , normal saline and ethanol are added dropwise till paclitaxelis completely dissolved ; 20 kg sodium alginate available in market is weighed and placed in the glass utensil , normal saline is added while stirring till sodium alginate is completely dissolved ; the mixed solution is extracted by aseptic syringe and added into the calcium chloride solution under the high - voltage electrostatic droplet device . the sodium alginate microspheres containing paclitaxel settles at the bottom of the glassware with diameters of 400 ˜ 750 μm . alternatively , the wet - microspheres can be used directly after the decanter of the upper solution and washed twice . as for patients with hysteromyoma , by adopting intervention radiotherapy or intervention ultrasound , insert the duct into feeding artery of target organ to conduct arteriography . paclitaxel - containing sodium alginate microsphere with diameter ranging from 400 - 750 μm is selected according to the impression of arteriography . try to use micro - duct to carry out ultraselective embolus with aseptic operation . extract cacl 2 solution from the bottle using injector and add in equal volume normal saline to rinse paclitaxel - containing sodium alginate microsphere ( wet ball ) for 3 times , or extract the described cacl 2 solution from the bottle and add in equal volume normal saline , pour normal saline and microsphere into an aseptic bowl . rinse the microsphere using 50 - 60 ml normal saline once and discard the rinse solution , and then add in appropriate amount or diluted contrast medium to mix up evenly ( leaving microsphere thoroughly floating in the contrast medium ), and infuse it into the foci slowly or slowly in multiple times depending on concrete conditions by duct under fluoroscopy ( prohibit to overdose of embolism .) till flow rate of contrast medium is obviously reduced , i . e . embolism is finished . arteriography is conducted again to judge the effect of embolism . the clean glass utensil is dried then keeped in oven at 300 ° c . for 3 hours ( the heat source is removed till the bacteria are killed ); 2 kg paclitaxel available in market is weighed and placed in the glass utensil mentioned above , acetone is added dropwise till paclitaxelis completely dissolved ; 150kg sodium alginate available in market is weighed and placed in the glass utensil , normal saline is added while stirring till sodium alginate is completely dissolved ; the mixed solution is extracted by aseptic syringe and added into the calcium chloride solution under the high - voltage electrostatic droplet device . the sodium alginate microspheres containing paclitaxel settles at the bottom of the glassware with diameters of 600 ˜ 950 μm . the upper solution is decanted and the lower microgel particles are put into the oven and keeped in a sealed condition . the diameters of dry - microspheres are 400 ˜ 750 μm . prior to use , the dryp - microspheres should be rehydrated with normal saline for a couple of minutes . alternatively , the wet - microspheres can be used directly after the decanter of the upper solution and washed twice . as for patients with hysteromyoma , by adopting intervention radiotherapy or intervention ultrasound , insert the duct into feeding artery of target organ to conduct arteriography . paclitaxel - containing sodium alginate microsphere with diameter ranging from 600 - 750 μm is selected according to the impression of arteriography . try to use ultraselective micro - duct to carry out embolus with aseptic operation while using . extract cacl 2 solution from the bottle using injector and add in equal volume normal saline to rinse paclitaxel - containing sodium alginate microsphere ( wet ball ) for 3 times , or extract the described cacl 2 solution from the bottle and add in equal volume normal saline , pour normal saline and microsphere into an aseptic bowl . rinse the microsphere using 50 - 60 ml normal saline once and discard the rinse solution , and then add in appropriate amount or diluted contrast medium to mix up evenly ( leaving microsphere thoroughly floating in the contrast medium ), and infuse it into the foci slowly or slowly in multiple times depending on concrete conditions by duct under fluoroscopy ( prohibit to overdose of embolism .) till flow rate of contrast medium is obviously reduced , i . e . embolism is finished . arteriography is conducted again to judge the effect of embolism . sodium alginate , used as a drug carrier in this invention is natural extracts , which is a kind of polysaccharide sodium salt composed byβ - d - mannitol and α - l - gulose extracted from natural brown algae . as a kind of linear macromolecule , its molecular weight is 50 , 000 - 100 , 000 dalton . it has high hydrophilicity and can be easily dissolved in water forming viscous colloid . cross - link and solidification among macromolecular links may occur under the action of calcium ion . it can be processed into solid spherical or spherical like microsphere in different sizes according to clinical necessity . this kind of microsphere has good biocompatibility . in vivo the living organism , calcium ion may be gradually educed , and microsphere is nontoxicly degraded within 3 - 6 months due to molecular chain scission . no debris is produced during degradation , and it may also result in the eternal vascular embolism in target organs ( when embolus remains in the vessel as long as 2 months , the intravascular thrombus may form and achieve the objective of eternal embolism . ), thus to achieve the goal of treatment . in practical operation , using this kind of “ biological multifunctional microsphere ” embolism materials , by physical clogging tumor or arteriole vessel around the treated portion , may cause vascular closure , block blood supply and nutrients of the tissue in that portion , thus to induce atrophy and necrosis due to 1 ischemia and hypoxia . meanwhile , it may provide advantageous condition for operation by reducing blood supply of target organs . therefore , it shows dual therapeutic effect of embolism and drugs by taking this microsphere as drug carriers in treatment of gynecological diseases to slowly release to the tissue of local foci in a timing , positioning , and orientation way , thus to improve therapeutic efficacy greatly . in the present invention , sodium alginate is selected as carriers added with antitumor target medicine , locally releasnig target medicine in a timing , positioning , and orientation way to kill tumor cells and arrive at treatment objective in light of peculiar anticancer mechanism and clinical application of paclitaxel , semi interpenetrating network structure and degradable principle of sodium alginate microsphere by combining previous clinical practice of sodium alginate microsphere embolus , taking safety , nontoxicity , non - immunogenicity , no inherent toxicity , nontoxic in generation , non - carcinogenicity , etc . into consideration . in the study of sodium alginate microshere vascular embolus containing paclitaxel , paclitaxel is not dissolved in water , the packed paclitaxel droplet will not precipitate and form no microsphere with no crystal educed . these procedures are completed in the study . after adding special mixture reagent , organic solvent , etc ., adjusting concentration , frequency and volecity , the microsphere , very well packed is even , round and smooth with evenly scattering medicine . the medicine is carried by microsphere to protect medicinal active group to maintain in vivo stability in internal environment , avoid the leakage of paclitaxel from human body too early and too rapidly , thus to arrive at the requirements for clinical application . sodium alginate microshere vascular embolus containing paclitaxel in the present invention has become the most promising target releasing medicine system attributable to large carrier amount of medicinal microsphere , longer in vivo residence time , target exclusive . when the concentration of paclitaxel is 10 mol / l , no paclitaxel crystal or medicinal microsphere accumulation in the produced medicinal microsphere has been observed by phase contrast microscope . besides , it still keeps good physical and chemical stability at 4 □ for 30 days . when the concentration is 30 mol / l , most paclitaxel crystal and medicinal microsphere accumulation can be observed under microscope . microsphere granule surface made of sodium alginate has certain negative charge , rejecting each other among granules . in application , dosage should be selected by the foci . intervention radiotherapy or intervention ultrasound may be adopted to insert the duct into feeding artery of target organ . mix the medicinal microsphere and contrast medium using injector after arteriography to slowly inject . it will not aggregate in the duct and block the duct .
vascular embolus of paclitaxel - sodium alginate microsphere and its preparation . the preparation of it consists of the following steps : preparing the solution of paclitaxel , sodium alginate and dilvalent metal cations such as bacl 2 or cacl 2 etc . by order ; mixing the paclitaxel solution and sodium alginate solution , and then mixing them with the curing solution of bacl 2 or cacl 2 and the like . then the obtained is the sodium alginate microshere vascular embolus containing paclitaxel . the carrier materials of the vascular embolus according to the invention are natural extracts and have good biocompatibility and re biodegradable ; the side effect of the pharmaceutic loaded by them is minimal , and it is therefore suitable for the vascular embolism and chemotherapy against the tumor .