the beverage composition in its broadest aspect comprises skim ( fat free ) milk centrifuged to separate the milk into a plasma ( liquid ) phase and a milk solids ( solid ) phase . the liquid phase , once separated , is filtered through an ultra - filtration filter having a pore size in the range of from about 0 . 05 to about 10 μm at a pressure of from about 0 to about 145 psi to form a first filtrate and a first retentate . the first filtrate is used in a subsequent step disclosed hereinbelow . the first retentate ( primarily lactose ) is reserved for further processing as a byproduct of the process . in an alternative embodiment , liquid skim milk or nonfat dry milk ( skim ) powder may be used . if skim powder is used , it is reconstituted to skim milk specifications with ultrafiltered tap or reverse osmosis ( ro ) purified water . either alternative is centrifuged to separate it into a liquid phase and a milk solids phase . the milk solids phase having a first volume is rinsed by mixing thoroughly at low shear into three times the first volume of reverse osmosis ( ro ) water carbonated to a level of about 1 . 5 volumes of co 2 to form a second filtrate and a second retentate . the solid phase is mixed with the carbonated water from about 4 to about 30 minutes . the mixture is next filtered through an ultra - filtration filter having a pore size of from about 0 . 05 to about 10 μm at a pressure of from about 0 to about 145 psi to form a second filtrate and a second retentate . the second filtrate and the second retentate from this step are reserved for further processing . the first filtrate is next treated with co 2 gas in a closed container at a temperature of from about 35 to about 38 ° f . until the volume of gas dissolved in the filtrate reaches from about 3 to about 4 volumes . the resulting liquid / gas mixture is mixed at low shear to removed dissolved o 2 for a period of about 5 minutes . the liquid / gas mixture is next combined with the second filtrate and the second retentate while maintaining the temperature of the resulting mixture at about 90 ° f . in another aspect of the disclosure , about three times the skim milk , in original liquid or reconstituted powder form , is placed in a closed vessel fitted with a hepa filter system with 99 . 997 % efficiency to remove any airborne particulates or bacteria larger than 0 . 2 microns in size . the skim milk is then chilled from about 30 ° f . to about 33 ° f . for three ( 3 ) to six ( 6 ) hours at atmospheric pressure under controlled conditions so that a percentage of the lactose sugar in the milk is crystallized ( approximately 70 %) with an average crystal size of from about 200 to about 250μ . the crystallized lactose is removed by mechanical separation via ultrafiltration . it should be understood that the percentage of lactose crystallized is not critical to its removal as any lactose remaining after crystallization and mechanical removal is addressed with the following chemical means . to eliminate the remainder of the lactose in this alternative embodiment ( approximately 30 % of the original lactose content per volume of milk ), about 3 . 32 ml / gal of lactase enzyme ( aspergillus ) is added to the fluid permeate to hydrolyze the remaining lactose , a disaccharide , into two monosaccharides — glucose and galactose . the permeate / lactase mixture is incubated at about 40 ° f . for about 20 hours until hydrolysis is complete . after the lactose hydrolysis step is complete , the essentially lactose - free skim milk is heated to about 90 ° f . in a steam jacketed kettle or blending tank . to add sweetness and flavor to any of the foregoing milk embodiments including the skim milk embodiment , natural and / or artificial sweeteners and / or flavors are added . for each gallon of pasteurized , lactose - free skim milk prepared in accordance with the foregoing procedure , from about 0 . 1 to about 10 lbs of crystalline fructose , from about 0 . 1 to about 10 oz . of a flavoring agent selected from the group consisting of natural and / or artificial orange , natural and / or artificial lemon , natural and / or artificial strawberry , natural and / or artificial pomegranate , natural and / or artificial acai , natural and / or artificial goji berry , natural and / or artificial cola , natural and / or artificial saskatoon berry , natural and / or artificial vanilla , vanilla extract or vanillin , natural and / or artificial grape , natural and / or artificial lime , natural and / or artificial ginger , natural and / or artificial root beer , natural and / or artificial birch beer , natural and / or artificial sarsaparilla , natural and / or artificial tangerine , natural and / or artificial mango , natural and / or artificial peach , natural and / or artificial cherry , natural and / or artificial apple , natural and / or artificial banana , natural and / or artificial white , black and / or green teas , natural and / or artificial chocolate , natural and / or artificial kiwi , natural and / or artificial coconut , natural and / or artificial blueberry , natural and / or artificial raspberry , natural and / or artificial cranberry , natural and / or artificial guarbana and / or mixtures thereof are added . to provide optional coloring to the beverage , coloring agents may be added to the skim milk prepared as described above . for each gallon of pasteurized , lactose - free skim milk are added from about 0 . 001 % to about 0 . 9 % of a natural and / or artificial metachromatic coloring agent may be added . the coloring agent is selected from the group consisting of red beet root , aronia fruit anthocyanins from grapes , elderberries , black carrots , red cabbage , hibiscus , purple sweet potatoes , blueberries and saskatoon berries and / or mixtures thereof . additional coloring agents include anatto extract , beta carotene , beet juice , turmeric , fd & amp ; c yellow 5 ( tartrazine ), fd & amp ; c yellow 6 ( sunset yellow ), fd & amp ; c blue 1 ( brilliant blue fcf ), fd & amp ; c blue 2 ( indigotine ), fd & amp ; c red 3 ( erythrosine ), fd & amp ; c red 40 ( allura red ) and / or mixtures thereof . to any of the milk - based formulations , from about 100 to about 400 iu of vitamin d palmitate ( d3 ), may be added by mixing the vitamin into the mixture . the addition of vitamin d may be formed before , after , or during , the addition of any of the other ingredients disclosed herein . one benefit of the addition of colorants is as an indicator of product spoilage . if product spoilage occurs due to bacterial growth , a ph change out of the range from about 6 . 7 to about 4 . 9 will take place in the product and lead to a color change , indicative of product spoilage . the resulting blend of the pasteurized , lactose - free skim milk , sweetener and optional flavoring and / or coloring is well mixed at very low shear while infusing filtered co 2 gas in a closed container to adjust the dissolved co 2 content to about 1 . 5 volumes . prior to administration of the co 2 gas , all solid , liquid and gaseous impurities , e . g ., oils , aromatic hydrocarbons , aldehydes , sulfur compounds and / or bacteria are removed from the gas . the additional co 2 gas displaces any trapped air present in the blend prior to pasteurization . the gas also increases the efficiency of pasteurization by the complete removal of aerobic bacteria without the use of heat so as to preserve high heat labile nutrients ( vitamins , enzymes , etc .) contained in the milk base of the blend and to avoid carmalization of the added sugar sweetener , e . g . fructose . in an alternative aspect of the disclosure , optionally , the blend next may be passed through an htst pasteurizer at from about 165 ° to about 170 ° f . for about 25 seconds with no homogenization . the resulting product is collected in a sterile container and chilled to from about 32 ° to about 35 ° f . a final mixture of co 2 gas and nitrogen gas is added to the mixture where the partial pressure of co 2 gas is from about 1 % to about 99 % of the total gas mixture and the partial pressure of nitrogen is from about 1 % to about 99 %, and where the gas mixture is infused at a pressure of about 10 lb ./ in . 2 . this assures final removal of any trapped air or oxygen that can support aerobic bacterial growth . after each degassing , any dissolved air rises to the top and is removed with a bleed - off valve . this procedure may be repeated for up to about five ( 5 ) minutes to assure the complete removal of trapped air . after degassing , filtered co 2 gas ( approximately 100 %) is applied to the product in a closed chamber with constant flow adjusted to about 10 lb ./ in . 2 until a volume of dissolved co 2 of from about 1 . 0 to about 3 . 5 volumes of co 2 gas is achieved . during the co 2 gas infusion , the temperature of the product blend must be maintained at about 32 ° to about 35 ° f . to assure effective and uniform carbonization . the product is then checked for the following final co 2 gas content before bottling under aseptic conditions into any size containers including 8 oz . and 12 oz . pet bottles . the resulting product may include per 8 oz ., calcium from about 34 to about 40 % rdi , protein from about 8 to about 10 g , co 2 gas from about 1 . 0 to about 3 . 5 volumes ( the higher the co 2 content , the lower the ph ), ph from about 6 . 5 to about 4 . 9 and calories at about 70 . in a further aspect of the disclosure , from about 0 . 1 to about 4 volumes of nitrogen is mixed with from about 0 . 1 to about 4 volumes of carbon dioxide per 8 oz . of beverage solution . the final co 2 / n 2 level may be adjusted from about 1 to about 2 volumes . alternatively , the final co 2 / n 2 level may be adjusted to about 1 . 5 volumes . the addition of nitrogen with the carbon dioxide exhibits several advantages . the nitrogen / carbon dioxide mixture provides additional protection from oxygen pick - up by the beverage . the mixture also reduces the amount of carbon dioxide needed to prevent bacterial and / or mold growth , which extends shelf life . the nitrogen component has a low solubility , less than 2 %, which means it will leave the beverage when the container is opened . the carbon dioxide component has a higher solubility and remains in the beverage to provide the desired sensory benefits . another added benefit is that the presence of nitrogen reduces the amount of carbon dioxide needed , which , in turn , lowers the acidity without affecting the extended shelf life . additional benefits include improvement in texture , taste and appearance of the beverage as well as ice - crystal growth inhibition in low temperature beverage storage . there are at least two ways in which the nitrogen may be introduced into the beverage . the first is to introduce the nitrogen in liquid form . the second is by gas sparger . whichever option is chosen , each is added after any pasteurization step . it is to be understood that the sequence of adding the ingredients as set forth herein is not essential to the production of the beverage mixture with one exception . it is important that the calcium salt ( s ) be added to the mixture before the magnesium salt ( s ) to prevent undesired clumping . having described the invention , it should be understood that the foregoing description of the invention is intended merely to be illustrative thereof and that other modifications , embodiments and equivalents may be apparent to those who are skilled in the art without departing from its spirit .
carbonated dairy nutrient beverage solutions that supply essential nutrients in the human diet are disclosed . the solutions may contain per 8 oz ., calcium , magnesium and potassium ions in the form of salts , vitamins a , d , c , and optionally lutein , zeaxanthin and folic acid in specified amounts to provide dietary supplementation . sweeteners , stabilizers and flavors can also be added to enhance flavor , sensory appeal , mouth - feel , ingredient solubilization and product appearance . a method of making the beverages is also described . a method of using carbon dioxide and nitrogen to reduce bacterial counts and reduce degradation of essential nutrients in dairy beverages with or without pasteurization is also disclosed .