provided herein is a method for the preparation of liposomes , the method comprising : ( a ) combining one or more lipids in an aqueous medium at ambient temperature ; ( c ) adding one or more sugars to the resulting mixture , thereby forming a solution of liposomes . in another embodiment , at step ( b ), the method further comprises homogenization of the lipids in the aqueous medium . some embodiments further comprise a step ( d ), wherein one or more active agents is added to the solution of liposomes . in one embodiment , the active agent is a hydrophobic drug . in one embodiment , the active agent is added as a solid . in another embodiment , the active agent is added in an organic solvent . in another embodiment , the active agent is added in organic solvent which further comprises one or more fatty acid salts , fatty acids and / or phospholipids . in another embodiment , the active agent is added during formation of the liposome . in certain embodiments , the resulting liposomes are less than about 1 μm in diameter . in one embodiment , the resulting liposomes are less than about 500 nm in diameter . in one embodiment , the resulting liposomes are less than about 100 nm in diameter . in one embodiment , at least one of the lipids is a phospholipid or a mixture of phospholipids . examples of phospholipids include , but are not limited to , phosphatidic acid (“ pa ”), phosphatidylcholine (“ pc ”), phosphatidylglycerol (“ pg ”), phophatidylethanolamine (“ pe ”), phophatidylinositol (“ pi ”), and phosphatidylserine (“ ps ”), sphingomyelin ( including brain sphingomyelin ), lecithin , lysolecithin , lysophosphatidylethanolamine , cerebrosides , diarachidoylphosphatidylcholine (“ dapc ”), didecanoyl - l - alpha - phosphatidylcholine (“ ddpc ”), dielaidoylphosphatidylcholine (“ depc ”), dilauroylphosphatidylcholine (“ dlpc ”), dilinoleoylphosphatidylcholine , dimyristoylphosphatidylcholine (“ dmpc ”), dioleoylphosphatidylcholine (“ dopc ”), dipalmitoylphosphatidylcholine (“ dppc ”), distearoylphosphatidylcholine (“ dspc ”), 1 - palmitoyl - 2 - oleoyl - phosphatidylcholine (“ popc ”), diarachidoylphosphatidylglycerol (“ dapg ”), didecanoyl - l - alpha - phosphatidylglycerol (“ ddpg ”), dielaidoylphosphatidylglycerol (“ depg ”), dilauroylphosphatidylglycerol (“ dlpg ”), dilinoleoylphosphatidylglycerol , dimyristoylphosphatidylglycerol (“ dmpg ”), dioleoylphosphatidylglycerol (“ dopg ”), dipalmitoylphosphatidylglycerol (“ dppg ”), distearoylphosphatidylglycerol (“ dspg ”), 1 - palmitoyl - 2 - oleoyl - phosphatidylglycerol (“ popg ”), diarachidoylphosphatidylethanolamine (“ dape ”), didecanoyl - l - alpha - phosphatidylethanolamine (“ ddpe ”), dielaidoylphosphatidylethanolamine (“ depe ”), dilauroylphosphatidylethanolamine (“ dlpe ”), dilinoleoylphosphatidylethanolamine , dimyristoylphosphatidylethanolamine (“ dmpe ”), dioleoylphosphatidylethanolamine (“ dope ”), dipalmitoylphosphatidylethanolamine (“ dppe ”), distearoylphosphatidylethanolamine (“ dspe ”), 1 - palmitoyl - 2 - oleoyl - phosphatidylethanolamine (“ pope ”), diarachidoylphosphatidylinositol (“ dapi ”), didecanoyl - l - alpha - phosphatidylinositol (“ ddpi ”), dielaidoylphosphatidylinositol (“ depi ”), dilauroylphosphatidylinositol (“ dlpi ”), dilinoleoylphosphatidylinositol , dimyristoylphosphatidylinositol (“ dmpi ”), dioleoylphosphatidylinositol (“ dopi ”), dipalmitoylphosphatidylinositol (“ dppi ”), distearoylphosphatidylinositol (“ dspi ”), 1 - palmitoyl - 2 - oleoyl - phosphatidylinositol (“ popi ”), diarachidoylphosphatidylserine (“ daps ”), di decanoyl - l - alpha - phosphatidylserine (“ ddps ”), dielaidoylphosphatidylserine (“ deps ”), dilauroylphosphatidylserine (“ dlps ”), dilinoleoylphosphatidylserine , dimyristoylphosphatidylserine (“ dmps ”), dioleoylphosphatidylserine (“ dops ”), dipalmitoylphosphatidylserine (“ dpps ”), distearoylphosphatidylserine (“ dsps ”), 1 - palmitoyl - 2 - oleoyl - phosphatidylserine (“ pops ”), diarachidoyl sphingomyelin , didecanoyl sphingomyelin , dielaidoyl sphingomyelin , dilauroyl sphingomyelin , dilinolcoyl sphingomyelin , dimyristoyl sphingomyelin , sphingomyelin , dioleoyl sphingomyelin , dipalmitoyl sphingomyelin , distearoyl sphingomyelin , and 1 - palmitoyl - 2 - oleoyl - sphingomyelin . the phospholipids provided herein may be chiral or achiral . the chiral phospholipids provided herein may be d - or l - phospholipids , for example , l - α - phosphatidylcholine or l - 3 - phosphatidylcholine . in one embodiment , l - α - phosphatidylcholine is used in the methods provided herein . in another embodiment , provided herein is a method for the preparation of liposomes , the method comprising : ( a ) combining sodium oleate and l - α - phosphatidylcholine in an aqueous medium at ambient temperature ; ( c ) adding one or more sugars to the resulting mixture , thereby forming a solution of liposomes . in one embodiment , the aqueous medium contains one or more active agents , or pharmaceutically acceptable salts , hydrates , clathrates or prodrugs thereof . examples of active agents include , but are not limited to , lapachone ( β - lapachone ), taxanes ( including , but not limited to , taxol , 7 - epitaxol , 7 - acetyl taxol , 10 - desacetyltaxol , 10 - desacetyl - 7 - epitaxol , 7 - xylosyltaxol , 10 - desacetyl - 7 - sylosyltaxol , 7 - glutaryltaxol , 7 - n , n - dimethylglycycltaxol , 7 - l - alanyltaxol , taxotere , and mixtures thereof ), paclitaxel , colchicine , transferrin , cyclosporines , cyclosporin a , ketoprofen , propofol , acetylsalicylic acid , acetaminophen , amphotericin , digoxin , doxorubicin , daunorubicin , epirubicin , idarubicin , angiogenesis inhibitors ( e . g , bevacizumab , ranibizumab , vitaxin , carboxyamidotriazole , combretastatin a - 4 , fumagillin analogs ( e . g ., tnp - 470 ), cm101 , ifn - α , interleukin - 10 , interleukin - 12 , platelet factor - 4 , suramin , su5416 , thrombospondin , vegfr antagonists , angiostatin , endostatin , 2 - methoxyestradiol , tecogalan , thalidomide , prolactin , linomide , angiopoietin - 1 , basic fibroblast growth factor , vascular endothelial growth factor ), vinca - alkaloids ( e . g ., vinblastine , vincristine , vindesin , etoposide , etoposide phosphate , and teniposide ), cytarabine , actinomycin , etoposide , bleomycin , gentamycin , cyclophosphamide , methotrexate , streptozotocin , cytosine , β - d - arabinofuranoside - 5 ′- triphosphate , cytochrome c , cisplatin , n - phosphono - acetyl - l - aspartic acid , 5 - fluoroorotic acid , acyclovir , zidovudine , interferons , aminoglycosides , cephalosporins , tetracyclines , propranolol , timolol , labetolol , clonidine , hydralazine , imipramine , amitriptyline , doxepim , phenyloin , diphenhydramine , chlorphenirimine , promethazine , prostaglandins , methotrexate , progesterone , testosterone , estradiol , estrogen , epirubicin , beclomethasone and esters , vitamin e , cortisone , dexamethasone and esters , betamethasone valerete , biphenyl dimethyl dicarboxylic acid , calcitonins , camptothecin , captopril , cephazoline , chloroquinine , chlorothiazole , co - agulation factors viii and ix , d - alpha - tocopherol , dexamethasone , dichlofenac , etoposide , feldene , flubiprofen , 5 - fluorouracil , fluoxetine , fusidic acid , gentamicin , glyburide , granisetron , growth hormones , indomethacin , insulin , itraconazole , ketoconazole , methotrexate , metronidazole , minoxidil , mitomycin , nafcillin , naproxen , ondansetron , oxyphenbutazone , parazosin , physostigmine , piroxicam , prednisolone , primaquine , quinine , ramipril , taxotane , tenoxicam , terazosin , triamcinolone , urokinase , opioid analgesics ( e . g ., alfentanil , anileridine , codiene , diamorphine , fentanyl , hydrocodone , hydromorphone , meperidine , morphine , oxycodone , oxymorphone , propoxyphene , sufentanil , pentazocine and nalbuphine ), non - steroidal anti - inflammatory drugs ( e . g ., aspirin , indometacin , ibuprofen , mefenamic acid and phenylbutazone ), angiotensin converting enzyme (“ ace ”) inhibitors ( e . g ., captoprilpolyene ), protein kinase c inhibitors , antibiotics ( e . g ., imidazole and triazole antibiotics ), folic acid , anthracycline antibiotics , anti - sense rnas , tricathecums , microbial ribosomal - inactivating toxins ( e . g ., gelonin , abrin , ricin a chain , pseudomonas exotoxin , diptheria toxin , pokeweed antiviral peptide ), pipecolic acid derivatives ( e . g ., tacrolimus ), plant alkaloids , dyes , radioisotope - labeled compounds , radiopaque compounds , radiosensitizers ( e . g ., 5 - chloro - 2 ′- deoxyuridine , 5 - bromo - 2 ′- deoxyuridine and 5 - iodo - 2 ′- deoxyuridine ), fluorescent compounds , mydriatic compounds , bronchodilators , local anesthetics ( e . g ., dibucaine and chlorpromazine ), antifungal agents ( e . g ., miconazole , terconazole , econazole , isoconazole , butaconazole , clotrimazole , itraconazole , nystatin , naftifine and amphotericin b ), antiparasitic agents , hormones , hormone antagonists , immunomodulators , neurotransmitter antagonists , anti - diabetic agents , antiglaucoma agents , vitamins , narcotics , and imaging agents . for additional disclosure of active agents , see gilman et al ., goodman and gilman &# 39 ; s : the pharmacological basis of therapeutics , 10th ed ., mcgraw - hill , new york , 2001 ; the merck manual of diagnosis and therapy , berkow , m . d . et al . ( eds . ), 17th ed ., merck sharp & amp ; dohme research laboratories , rahway , n . j ., 1999 ; cecil textbook of medicine , 20th ed ., bennett and plum ( eds . ), w . b . saunders , philadelphia , 1996 . in one embodiment , the active agent is a hydrophobic compound , or a compound with poor solubility in water . in one embodiment , the active agent is a water soluble membrane - impermeant agent such as a peptide , a protein , a nucleic acid , a nucleotide , a nucleoside , a carbohydrate or an analog thereof . in one embodiment , the aqueous medium does not contain an active agent . in another embodiment , the resulting solution contains 10 % by weight trehalose . in another embodiment , the active agent is lapachone , or a pharmaceutically acceptable salt , hydrate , clathrate or prodrug thereof . in another embodiment , the active agent is transferrin , or a pharmaceutically acceptable salt , hydrate , clathrate or prodrug thereof . in another embodiment , the active agent is cyclosporine , or a pharmaceutically acceptable salt , hydrate , clathrate or prodrug thereof . in one embodiment , the active agents are transferrin and lapachone , or pharmaceutically acceptable salts , hydrates , clathrates or prodrugs thereof . examples of sugars that may be used in the methods provided herein include , but are not limited to , sucrose , glucose , fructose , lactose , maltose , mannose , galactose and trehalose . in one embodiment , the liposomal preparation is suitable for parenteral administration to a patient suffering from one or more diseases or disorders . in certain embodiments , the sequence of the addition of active agent ( s ) results in enhanced solubility of the active agent ( s ). the conventional method of incorporating hydrophobic drug to liposome is by adding the drug to lipid before liposome preparation . see , e . g ., immordino , m . l . et al ., journal of controlled release , 2003 , 91 : 417 - 429 . by the conventional process , the incorporation of the drug is only 0 . 3 to 0 . 7 mg / ml . provided herein are methods for the preparation of liposomes wherein the addition of the active agent ( s ) after fog illation of the liposome results in enhanced solubility of the active agent ( s ). in one embodiment , the solubility of the active agent ( s ) in liposome is increased by at least about two - fold , five - fold or ten - fold compared to the conventional process . in one embodiment , the solubility of the active agent ( s ) in liposome is increased to about 5 mg / ml . in certain embodiments in which the active agent is a hydrophobic drug , the active agent is added to pre - made liposome as a solid or in an organic solvent . in one embodiment , the pre - made liposome comprises one or more fatty acid salts , fatty acids and / or phospholipids to increase the solubility of the active agent . in one embodiment , the sequence of the addition of active agent ( s ) results in greater efficiency of incorporation of the active agent ( s ) into liposome . in certain embodiments , the efficiency of incorporation into liposome is 50 %, 60 %, 70 %, 80 %, 90 %, 95 %, 98 %, 99 % or 100 %. in certain embodiments , the efficiency of incorporation is 90 %, 95 %, 98 %, 99 % or 100 %. without being limited to a particular theory or mechanism , the increase in incorporation of active agent to pre - made liposome ( e . g ., to a concentration of about 5 mg / ml ) may be due to increase in surface area of liposome . also provided herein is a liposomal composition prepared by a method comprising : ( a ) combining one or more lipids in an aqueous medium at ambient temperature ; ( c ) adding one or more sugars to the resulting mixture , thereby forming a solution of liposomes . in another embodiment , provided herein is a liposomal composition prepared by a method comprising : ( a ) combining one or more lipids in an aqueous medium at ambient temperature ; ( c ) adding one or more sugars to the resulting mixture , thereby forming a solution of liposomes ; and in one embodiment , the active agent is added as a solid . in one embodiment , the active agent is added in an organic solvent . in one embodiment , the active agent in organic solvent further comprises one or more fatty acid salts , fatty acids and phospholipids . in certain embodiments , the methods provided herein result in stable solutions , compositions or formulations comprising liposomes and one or more active agents . in these embodiments , the active agent ( s ) remain solubilized for a specified amount of time and do not significantly degrade , aggregate or become otherwise modified ( e . g ., as determined by hplc ). in some embodiments , about 70 percent or greater , about 80 percent or greater or about 90 percent or greater of the active agent remains solubilized after a week after dilution with an acceptable diluent at an elevated temperature ( e . g ., about 35 ° c . or higher ). in other embodiments , about 70 percent or greater , about 80 percent or greater or about 90 percent or greater of the active agent remains solubilized after a week after dilution with an acceptable diluent at room temperature . in other embodiments , about 70 percent or greater , about 80 percent or greater or about 90 percent or greater of the active agent remains solubilized after a week at a reduced temperature ( e . g ., about 10 ° c . or lower ). in certain embodiments , the methods provided herein result in enhanced solubility of an active agent , as compared to the solubility of the same active agent in an aqueous medium . specifically , when the methods provided herein are used , the solubility of the active agent increases about 20 percent or more , about 40 percent or more , about 60 percent or more , about 80 percent or more , about 100 percent or more , or about 200 percent or more of the solubility of the same active agent in a reference solvent . in some embodiments , the reference solvent is water . also provided herein is a method of treating a disease or disorder using a liposomal composition provided herein . in some embodiments , the disease or disorder includes , but is not limited to , oncological disorders , proliferative disorders , central nervous system disorders , autoimmune disorders , and inflammatory diseases or disorders . in other embodiments the methods are directed to the treatment of bacterial , viral or fungal infections . proliferative disorders ( e . g . cancer ) that may be treated by the methods provided herein include , but are not limited to , neoplasms , tumors ( malignant and benign ) and metastases , or any disease or disorder characterized by uncontrolled cell growth . the cancer may be a primary or metastatic cancer . specific examples of cancers that can be prevented , managed , treated or ameliorated in accordance with the methods of the invention include , but are not limited to , cancer of the head , neck , eye , mouth , throat , esophagus , chest , bone , lung , colon , rectum , stomach , prostate , breast , ovaries , kidney , liver , pancreas , and brain . additional cancers include , but are not limited to , the following : leukemias ( e . g ., acute leukemia , acute lymphocytic leukemia ), acute myelocytic leukemias ( e . g ., myeloblastic , promyelocytic , myelomonocytic , monocytic , erythroleukemia leukemias and myelodysplastic syndrome ), chronic leukemias ( e . g ., chronic myelocytic ( granulocytic ) leukemia , chronic lymphocytic leukemia , hairy cell leukemia ), polycythemia vera , lymphomas ( e . g ., hodgkin &# 39 ; s disease , non - hodgkin &# 39 ; s disease ), multiple myelomas ( e . g ., smoldering multiple myeloma , nonsecretory myeloma , osteosclerotic myeloma , plasma cell leukemia , solitary plasmacytoma and extramedullary plasmacytoma ), waldenstrom &# 39 ; s macroglobulinemia , monoclonal gammopathy of undetermined significance , benign monoclonal gammopathy , heavy chain disease , bone and connective tissue sarcomas ( e . g ., bone sarcoma , osteosarcoma , chondrosarcoma , ewing &# 39 ; s sarcoma , malignant giant cell tumor , fibrosarcoma of bone , chordoma , periosteal sarcoma , soft - tissue sarcomas , angiosarcoma ( hemangiosarcoma ), fibrosarcoma , kaposi &# 39 ; s sarcoma , leiomyosarcoma , liposarcoma , lymphangiosarcoma , neurilemmoma , rhabdomyosarcoma , synovial sarcoma ), brain tumors ( e . g ., glioma , astrocytoma , brain stem glioma , ependymoma , oligodendroglioma , nonglial tumor , acoustic neurinoma , craniopharyngioma , medulloblastoma , meningioma , pineocytoma , pineoblastoma , primary brain lymphoma ), breast cancer ( e . g ., adenocarcinoma , lobular ( small cell ) carcinoma , intraductal carcinoma , medullary breast cancer , mucinous breast cancer , tubular breast cancer , papillary breast cancer , paget &# 39 ; s disease , and inflammatory breast cancer ), adrenal cancer ( e . g ., pheochromocytom and adrenocortical carcinoma ), thyroid cancer ( e . g ., papillary or follicular thyroid cancer , medullary thyroid cancer and anaplastic thyroid cancer ), pancreatic cancer ( e . g ., insulinoma , gastrinoma , glucagonoma , vipoma , somatostatin - secreting tumor , and carcinoid or islet cell tumor ), pituitary cancers ( e . g ., cushing &# 39 ; s disease , prolactin - secreting tumor , acromegaly , and diabetes insipius ), eye cancers ( e . g ., ocular melanoma such as iris melanoma , choroidal melanoma , and cilliary body melanoma , and retinoblastoma ), vaginal cancers ( e . g ., squamous cell carcinoma , adenocarcinoma , and melanoma ), vulvar cancer ( e . g ., squamous cell carcinoma , melanoma , adenocarcinoma , basal cell carcinoma , sarcoma , and paget &# 39 ; s disease ), cervical cancers ( e . g ., squamous cell carcinoma , and adenocarcinoma ), uterine cancers ( e . g ., endometrial carcinoma and uterine sarcoma ), ovarian cancers ( e . g ., ovarian epithelial carcinoma , borderline tumor , germ cell tumor , and stromal tumor ), esophageal cancers ( e . g ., squamous cancer , adenocarcinoma , adenoid cyctic carcinoma , mucoepidermoid carcinoma , adenosquamous carcinoma , sarcoma , melanoma , plasmacytoma , verrucous carcinoma , and oat cell ( small cell ) carcinoma ), stomach cancers ( e . g ., adenocarcinoma , fungaling ( polypoid ), ulcerating , superficial spreading , diffusely spreading , malignant lymphoma , liposarcoma , fibrosarcoma , and carcinosarcoma ), colon cancers , rectal cancers , liver cancers ( e . g ., hepatocellular carcinoma and hepatoblastoma , gallbladder cancers such as adenocarcinoma ), cholangiocarcinomas ( e . g ., pappillary , nodular , and diffuse ), lung cancers ( e . g ., non - small cell lung cancer , squamous cell carcinoma ( epidermoid carcinoma ), adenocarcinoma , large - cell carcinoma and small - cell lung cancer ), testicular cancers ( e . g ., germinal tumor , seminoma , anaplastic , classic ( typical ), spermatocytic , nonseminoma , embryonal carcinoma , teratoma carcinoma , choriocarcinoma ( yolk - sac tumor ), prostate cancers such as but not limited to , adenocarcinoma , leiomyosarcoma , and rhabdomyosarcoma ), penile cancers , oral cancers ( e . g ., squamous cell carcinoma ), basal cancers , salivary gland cancers ( e . g ., adenocarcinoma , mucoepidermoid carcinoma , and adenoidcystic carcinoma ), pharynx cancers ( e . g ., squamous cell cancer , and verrucous ), skin cancers ( e . g ., basal cell carcinoma , squamous cell carcinoma and melanoma , superficial spreading melanoma , nodular melanoma , lentigo malignant melanoma , acral lentiginous melanoma ), kidney cancers ( e . g ., renal cell cancer , adenocarcinoma , hypernephroma , fibrosarcoma , transitional cell cancer ( renal pelvis and / or uterer )), wilms &# 39 ; tumor , bladder cancers ( e . g ., transitional cell carcinoma , squamous cell cancer , adenocarcinoma , carcinosarcoma ), myxosarcoma , osteogenic sarcoma , endotheliosarcoma , lymphangioendotheliosarcoma , mesothelioma , synovioma , hemangioblastoma , epithelial carcinoma , cystadenocarcinoma , bronchogenic carcinoma , sweat gland carcinoma , sebaceous gland carcinoma , papillary carcinoma and papillary adenocarcinomas , follicular lymphomas , carcinomas with p53 mutations , hormone dependent tumors of the breast , prostate and ovary , precancerous lesions such as familial adenomatous polyposis , and myelodysplastic syndromes . other specific diseases and disorders that may be treated by the methods provided herein include , but are not limited to , the following : allergic disorders , inflammation , asthma , arthritis , encephalitis , rheumatoid arthritis , osteoarthritis , psoriatic arthritis , inflammatory osteolysis , chronic or acute obstructive pulmonary disease , chronic or acute pulmonary inflammatory disease , inflammatory bowel disease , crohn &# 39 ; s disease , gout , bechet &# 39 ; s disease , henoch - schonlein purpura (“ hsp ”), septic shock , sepsis , meningitis , colitis , inflammation due to reperfusion , psoriasis , fibrosis including pulmonary fibrosis , parkinson &# 39 ; s disease , bradykinesia , muscle rigidity , parkinsonian tremor , parkinsonian gait , motion freezing , depression ; defective long - term memory , rubinstein - taybi syndrome ( rts ), dementia , sleep disorders , insomnia , postural instability , hypokinetic disorders , hyperkinetic disorders , synuclein disorders , multiple system atrophies , striatonigral degeneration , olivopontocerebellar atrophy , shy - drager syndrome , motor neuron disease with parkinsonian features , lewy body dementia , tau pathology disorders , progressive supraneulear palsy , corticobasal degeneration , frontotemporal dementia ; amyloid pathology disorders , mild cognitive impairment , alzheimer disease , alzheimer disease with parkinsonism , wilson disease , hallervorden - spatz disease , chediak - hagashi disease , sca - 3 spinocerebellar ataxia , x - linked dystonia parkinsonism , huntington disease , prion disease , chorea , ballismus , dystonia tremors , amyotrophic lateral sclerosis (“ als ”), cns trauma , myoclonus , and diseases or disorders associated with undesired immune reaction ( e . g ., organ rejection associated with an organ transplant ). viral infections that may be treated by the methods provided herein include , but are not limited to , the following : human immunodeficiency virus (“ hiv ”), herpes simplex virus type 1 , herpes simplex virus type 2 , influenza viruses , influenza virus type a , influenze virus type b , parainfluenza virus , human papillomavirus (“ hpv ”), adenoviruses , rhinoviruses , hepatitis a virus , hepatitis b virus , hepatitis c virus , hepatitis d virus , hepatitis e virus , dengue fever , yellow fever , west nile virus , japanese encephalitis virus , gb virus a , gb virus - b , gb virus - c , bovine viral diarrhea virus (“ bvdv ”), classical swine fever virus ( i . e ., hog cholera virus ), border disease virus , varicella zoster virus , smallpox , measles , rabies virus , arbovirus , cytomegalovirus , mumps virus , poliovirus , coxsackie b virus , epstein - barr virus , rubella virus , parvovirus b19 , coronaviruses ( e . g ., sars coronavirus ), astrovirus , norovirus , rotavirus , and adenoviruses . fungal infections that may be treated by the methods provided herein include , but are not limited to , aspergillosis , blastomycosis , coccidioidomycosis , cryptococcosis , fungal sinusitis , histoplasmosis , hypersensitivity pneumonitis , mucormycosis , paracoccidioidomycosis , sporotrichosis , and valley fever . bacterial infections that may be treated by the methods provided herein include , but are not limited to , brucellosis , cholera , leprocy , leptospirosis , shigellosis , trench fever , tularemia , q fever , whitmore &# 39 ; s disease , yersiniosis , yaws , vibrio vulnificus infections , streptococcus infections , staphylococcus infections and e . coli infections . 6 g of l - α - phosphatidylcholine ( soy ) was dispersed in 100 ml of water using a magnetic stirrer at 200 rpm for 10 minutes at ambient temperature . the dispersed liposome ( multilayer ) was passed through a microtluidic homogenizer at 15 , 000 psi . three cycles of passing resulted in a liposome less than 100 nm in diameter . trehalose was then added to the liposome to a final concentration of 10 % ( w / w ). the resulting stable isotonic liposome was either used as liquid or lyophilized . 200 mg of lapachone and 6 g of l - α - phosphatidylcholine ( soy ) were dispersed in 100 ml of water using a magnetic stirrer at 200 rpm for 10 minutes at ambient temperature . the dispersed liposome ( multilayer ) was passed through a microfluidic homogenizer at 15 , 000 psi . three cycles of passing resulted in a liposome encapsulated with 2 mg / ml lapachone less than 100 nm in diameter . trehalose was then added to the liposome to a final concentration of 10 % ( w / w ). the resulting stable isotonic liposome encapsulated with lapachone was either used as liquid or lyophilized . 500 mg of cyclosporine in 5 ml mygliol and 6 g of l - α - phosphatidylcholine ( soy ) were dispersed in 100 ml of water using a magnetic stirrer at 200 rpm for 10 minutes at ambient temperature . the dispersed liposome ( multilayer ) was passed through microfluidic homogenizer at 15 , 000 psi . three cycles of passing resulted in a liposome encapsulated with 5 mg / ml cyclosporine less than 100 nm in diameter . trehalose was then added to the liposome to a final concentration of 10 % ( w / w ). the resulting stable isotonic liposome formulation encapsulated and micro emulsified with cyclosporine was either used as liquid or lyophilized . 200 mg of transferrin and 6 g of l - α - phosphatidylcholine ( soy ) were dispersed in 100 ml of water using a magnetic stirrer at 200 rpm for 10 minutes at ambient temperature . the dispersed liposome ( multilayer ) was passed through a microfluidic homogenizer at 15 , 000 psi . three cycles of passing resulted in a liposome encapsulated with 5 mg / ml transferrin less than 100 nm in diameter . trehalose was then added to the liposome to a final concentration of 10 % ( w / w ). the resulting stable isotonic liposome formulation encapsulated with transferrin was either used as liquid or lyophilized . 6 mg of sodium oleate , 10 g of trehalose and 6 g l - α - phosphatidylcholine ( soy ) were dispersed in 100 ml of water using a magnetic stirrer at 200 rpm for 10 minutes at ambient temperature . the dispersed liposome ( multilayer ) was passed 10 times through a microfluidic homogenizer at 15 , 000 psi . 100 μl of colchicine dissolved in acetone was spiked into 1 ml of the pre - made liposome and lyophilized . the resulting stable isotonic lyophilized liposome encapsulated with the drug is essentially free from organic solvent after lyophilization . after lyophilization , the product can be reconstituted as a 1 mg / ml , 2 mg / ml , 3 mg / ml or 4 mg / ml aqueous solution ( e . g ., water for injection ). all references cited herein are incorporated herein by reference in their entireties and for all purposes to the same extent as if each individual publication , patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes .
provided herein are methods for preparing liposomes and uses thereof . in certain embodiments , liposomes are prepared without using heat , organic solvents , proteins , and / or inorganic salts in the process . in certain embodiments , the liposomal preparation contains one or more active agents . in certain embodiments , the liposomal preparations are used in the treatment of diseases or disorders .