in accordance with a first aspect , the present invention thus provides a method of producing a functional food product comprising mixing , in a ratio within the range of 2 - 7 : i ) a combination of water and one or more other food grade materials , preferably in the form of an aqueous dispersion or solution thereof , with ii ) egg yolk , wherein the egg yolk is obtained by feeding poultry a diet enriched in at least one pharmacologically active nutrient , collecting the eggs produced by said poultry and taking therefrom the yolk . as used herein the expression “ functional food product ”, refers to a class of processed foods that claims to promote good or improved health , to prevent disease , or the like , beyond the basic function of food which is to supply nutrients to the body . by including a sufficient amount of the egg yolk obtained in accordance with the invention in a food product , this is typically achieved . the present functional food product typically is a so - called ready - to - use product , which means that the product does not require any further processing , such as cooking or baking or mixing with other ingredients , before it is suitable for consumption . in accordance with a preferred embodiment of the invention the functional food product is a product selected from the group of beverages , such as fruit flavoured drinks or dairy type drinks ; and desserts , such as puddings , custards or spoonable yoghurts . it is furthermore particularly preferred that the functional food product is provided in unit dosage form . a unit dosage form , in accordance with its regular meaning , refers to a form wherein predetermined portions of the product are provided in discrete packages , said portions corresponding to the amount to be taken per administration event . a particularly preferred example includes discrete packages containing a portion corresponding to the daily dosage . more in particular discrete packages are preferably provided containing a single portion to be consumed as a single “ shot ”, “ snack ”, “ refreshment ” or “ bite ”. in accordance with the above , a particularly preferred embodiment of the invention provides closed or sealed packages containing a functional food product as defined herein before in an amount of 50 - 150 ml , preferably 75 - 125 ml . however , in another embodiment that is encompassed by the invention , a package is provided containing a multitude of such dosages or portions of the functional food product , i . e . which are not in discrete unitary dosage packages . typically such packages contain the product in an amount of 250 - 1000 ml , preferably 500 - 1000 ml . the term “ comprising ”, as used herein , is meant not to be limiting to — any subsequently stated elements but rather to encompass non - specified elements of major or minor functional importance . in other words the listed steps , elements or options need not be exhaustive . whenever the words “ including ”, “ containing ” or “ having ” are used , these terms are meant to be equivalent to “ comprising ” as defined above . the term “ egg yolk ”, as used herein may refer simply to untreated egg yolk as separated from the egg white after a shell egg is broken , but also to heat sterilized egg yolk , frozen egg yolk that has been thawed , rehydrated egg yolk that is obtained by adding water to dried powdered egg yolk to the extent of normal egg yolk , and egg yolk subjected to various treatments such as lyso - conversion , decholesterolization , lyso - decholesterolization , etc . the “ term egg - yolk solids ” is intended to mean the solids present in natural egg yolk or in dried egg - yolk products such as those commonly used as ingredients in the food industry . the amounts of solids in egg yolk from chickens eggs and dried egg - yolk products , can be determined using known methods , and are typically about 46 % and about 96 %, respectively . in the art poultry is the category of domesticated birds kept for meat and eggs . these most typically are members of the order galliformes ( which includes chickens and turkeys ). preferably , in accordance with the invention the term poultry is used to refer to chickens . the term “ pharmacologically active nutrient ” refers to any substance that can be found in food products forming part of a normal healthy diet , usually in low or trace amounts , and having a pharmacological effect , meaning that it has therapeutic or prophylactic effectiveness . the term pharmacologically active nutrient thus does not encompass the bulk food ingredients which function as the energy sources and / or building blocks in the normal metabolic processes of the human or animal body . suitable examples of such pharmacologically active nutrients include omega - 3 fatty acids , especially dha ( docosahexaenoic acid ) and epa ( eicosapentaenoic acid ), vitamin d , folic acid , vitamin e , xanthophylls , iodine , selenium and zinc . in a preferred embodiment of the invention , the pharmacologically active ingredient is selected from epa , vitamin d , folic acid , vitamin e , xanthophylls , iodine and selenium , more preferably from vitamin d , folic acid , vitamin e , xanthophylls , iodine and selenium . in accordance with one particularly preferred embodiment of the present invention the pharmacologically active nutrient is a lipophilic pharmacologically active nutrient for above - stated reasons . as mentioned before , according to the present invention the egg yolk is obtained by feeding poultry a diet enriched in at least one pharmacologically active nutrient and collecting the eggs produced by said poultry and taking therefrom the yolk . as used herein “ enriched ” has the meaning of containing additional amounts of said pharmacologically active nutrient as compared to standard poultry diets . typically the diet in accordance with the invention is enriched with said pharmacologically active nutrient to the extent that it contains 125 %, preferably 150 %, more preferably 200 % and most preferably 300 % of the amount of said nutrient normally consumed , e . g . on a daily basis , by said poultry . in accordance with a preferred embodiment of the present invention , the poultry is fed a diet enriched in at least two , preferably at least three , more preferably at least four and most preferably at least five pharmacologically active nutrients in accordance with the invention . furthermore , in accordance with a particularly preferred embodiment of the invention the diet fed to the poultry comprises omega - 3 fatty acids in an amount of at least 5 g / kg of the diet , more preferably within the range of 10 - 50 g / kg ; and / or vitamin d in an amount of at least 1500 iu , more preferably within the range of 2000 - 5000 iu ; and / or folic acid in an amount of at least 10 mg / kg , more preferably within the range of 20 - 100 mg / kg ; and / or vitamin e in an amount of at least 20 mg / kg , more preferably within the range of 100 - 500 mg / kg ; and / or xantophylls in an amount of at least 10 mg / kg , more preferably within the range of 20 - 100 ppm ; and / or iodine in an amount of at least 0 . 75 mg / kg , more preferably within the range of 1 - 5 mg / kg ; and / or selenium in an amount of at least 0 . 2 mg / kg , more preferably within the range of 0 . 2 - 0 . 3 mg / kg and / or zinc in an amount of at least 30 mg / kg , preferably in an amount of at least 50 mg / kg . the expression “ iu ” as used herein stands for international units , which is commonly used in the art for expressing the quantity of e . g . a vitamin in terms of biological activity . typically feeding the poultry diets enriched in one or more nutrients as described here before will result in the poultry producing eggs containing omega - 3 fatty acids in an amount of at least 100 mg / 100 g , preferably at least 150 mg / 100 g ; and / or vitamin d in an amount of at least 1 μg / 100 g , preferably at least 1 . 5 μg / 100 g ; and / or folic acid in an amount of at least 150 μg / 100 g , preferably at least 250 μg / 100 g ; and / or vitamin e in an amount of at least 2 mg / 100 g , preferably at least 3 mg / 100 g ; and / or xantophylls in an amount of at least 500 μg / 100 g , preferably at least 700 μg / 100 g ; and / or iodine in an amount of at least 60 μg / 100 g , preferably at least 90 μg / 100 g ; and / or selenium in an amount of at least 10 μg / 100 g , preferably at least 15 μg / 100 g and / or zinc in an amount of at least 2 . 5 mg / 100 g , preferably at least 3 mg / 100 g . as stated before , these eggs are collected and the yolk is taken therefrom . typically the egg yolk is separated from the egg white using an automated process , although this is not an essential characteristic of the invention . a non - limiting example of an apparatus for performing this kind of processing has been described in ep 1205140 . in accordance with a preferred embodiment of the invention a method is provided as described above , wherein the combination of water and the at least one food - grade material on the one hand and the egg yolk on the other hand are mixed in a ratio within the range of 3 - 6 , most preferably within the range of 3 . 5 - 5 . the food grade material in accordance with the invention typically comprises at least one material selected from the group of carbohydrates , proteins and lipids . suitable examples of carbohydrates , proteins and lipids are as listed herein below . in accordance with a preferred embodiment of the invention the combination of water and the at least one food grade material are in the form of an aqueous dispersion or solution prior to mixing with the egg yolk . typically said dispersion or solution at least comprises a dissolved or dispersed carbohydrate protein or lipid material . typically said food - grade material is comprised in said aqueous dispersion or solution in an amount of at least 1 wt %, preferably at least 2 wt %, most preferably at least 5 wt %. typically said amount may be as high as 80 wt %. preferably it does not exceed 50 wt %, most preferably it does not exceed 25 wt %. in accordance with a particularly preferred embodiment of the invention , a method is provided as defined herein before , wherein egg yolk is combined with an aqueous dispersion selected from the group consisting of skimmed milk , semi - skimmed milk , buttermilk , fermented milk , especially yoghurt , soy drink or soy milk , fermented soy milk , fruit juices , fruit purees , syrups , vegetable juices , vegetable purees and combinations thereof . it was surprisingly found that the daily use of a product in accordance with the invention wherein egg yolk and butter milk have been combined does not give rise to significant alterations in plasma cholesterol levels , whereas the ( daily ) intake of a corresponding amount of egg enriched in an pharmacologically active ingredient did give rise to significant increases in plasma cholesterol . these findings are described in more detail in the examples . without wishing to be bound by any theory it is hypothesized that the absence of plasma cholesterol increasing effects may be ascribable to the polar lipids , especially sphingolipids , naturally found in dairy products such as butter milk . therefore , in a particularly preferred embodiment of the invention , a method is provided as defined herein before , wherein the combination of water and one or more other food grade materials comprises buttermilk , skimmed milk , semi - skimmed milk , fermented milk , especially yoghurt , fractions thereof and mixtures thereof . most preferably it comprises whole buttermilk or a buttermilk fraction . in another preferred embodiment of the invention , a method is provided as defined herein before , wherein the combination of water and one or more other food grade materials comprises polar dairy lipids , especially polar dairy lipids selected from the group of sphingolipids and phospholipids , preferably from the group of sphingolipids , e . g . ceramide and / or sphingomyelin . preferably the combination of water and at least one further food grade material comprises , based on the total weight of the functional food product , at least 0 . 05 wt %, more preferably at least 0 . 08 wt % of said polar dairy lipids . it is also hypothesized that the bacteria present in yoghurt and buttermilk may cause or contribute to the absence of adverse effects on plasma lipids and cholesterol observed . therefore another preferred embodiment of the invention concerns a product as defined herein before comprising an effective amount , e . g . 10 4 cfu / ml or more , preferably 10 5 cfu / ml or more , most preferably 10 6 cfu / ml or more , of lactic acid bacteria , more preferably bacteria selected from lactobacillus and lactococcus , more preferably from l . lactis , l . acidophilus , l . casei , l . reuteri , l . gasseri and l . delbrueckii , most preferably from l . acidophilus , l . delbrueckii and l . casei . optionally further ingredients may be added as desired . hence , in a preferred embodiment of the invention a method is provided as defined herein before , comprising the step of adding one or more additional ingredients selected from the group consisting of colouring agents , flavourings , sweeteners , preservatives , anti - oxidants , viscosity modifying agents , emulsifiers , food acids and combinations thereof . preferably the method of the invention additionally comprises the steps of mixing , e . g . using shear , and / or pasteurizing or sterilizing the mixture that is obtained in accordance with the above described method . in accordance with a preferred embodiment of the invention the method does not comprise a step wherein ethanol is added to the product or wherein the product is processed such as to produce a liquor or alcoholic beverage . in a particularly preferred embodiment of the invention a method is provided as defined herein before , said method further comprising filling a package , preferably a package as described herein before , with the functional food product and sterilizing or pasteurizing said package before or after closing or sealing said package . a second aspect of the invention concerns the functional food product that is obtainable by any embodiment of the method as defined herein before . the functional food product of the invention comprises a mixture of the egg yolk and a combination of water and at least one further food - grade material , as defined herein before . in accordance with a particularly preferred embodiment of the present invention a food product is provided wherein said combination of water and at least one further food - grade material is a dispersion selected from the group of skimmed milk , semi - skimmed milk , buttermilk , fermented milk , especially yoghurt , soy drink or soy milk , fermented soy milk , fruit juices , fruit purees , syrups , vegetable juices , vegetable purees and combinations thereof . as explained before , a particularly preferred embodiment of the invention concerns a product comprising buttermilk , skimmed milk , semi - skimmed milk , fermented milk , especially yoghurt , fractions thereof and mixtures thereof . another preferred embodiment of the invention concerns a product as defined before comprising one or more polar dairy lipids , especially polar dairy lipids selected from the group of sphingolipids and phospholipids , preferably from the group of sphingolipids . said lipids are preferably present in a total amount of at least 0 . 05 wt %, more preferably at least 0 . 08 wt %. another preferred embodiment of the invention concerns a product as defined before comprising an effective amount , e . g . 10 4 cfu / ml or more , preferably 10 5 cfu / ml or more , most preferably 10 6 cfu / ml or more , of lactic acid bacteria , more preferably of bacteria selected from lactobacillus and lactococcus , more preferably from l . lactis , l . acidophilus , l . casei , l . reuteri , l . gasseri and l . delbrueckii , most preferably from l . acidophilus , l . delbrueckii and l . casei . in a particularly preferred embodiment of the invention the functional food product comprises a combination of the egg yolk , water at least one further food - grade material selected from the group of carbohydrates , proteins and lipids , and optionally one or more additional ingredients such as colouring agents , flavourings , preservatives , anti - oxidants , viscosity modifying agents , e . g . starch or gelatine , emulsifiers , food acids and the like . it is noted that , although the functional food products of the invention do not require the addition of any emulsifying agent , due to the presence of the egg yolk phospholipids , products containing additional emulsifying agents , for whatever purpose , are encompassed by the present invention . in accordance with a preferred embodiment of the invention , a functional food product is provided as defined herein before , wherein the amount of egg yolk solids is within the range of 0 . 025 - 0 . 4 g / ml of the functional food product . in an even more preferred embodiment of the present invention a functional food product is provided wherein the amount of egg yolk solids is within the range of 0 . 035 - 0 . 3 g / ml , more preferably , most preferably 0 . 05 - 0 . 2 g / m 1 . as will become apparent from the description hereafter this roughly corresponds to the product containing the complete yolk of 1 chicken egg per 50 - 150 ml , preferably 75 - 125 ml . in this document , the amount of egg yolk is expressed as dry solids weight per milliliter of the functional food product . as will be understood by the skilled person , this is not intended to imply that egg yolk is necessarily added in dry solid form , although such an embodiment is encompassed by the claimed invention . furthermore , in accordance with a preferred embodiment , a functional food product as defined herein before is provided , containing omega - 3 fatty acids in an amount of at least 50 mg / 100 ml of the functional food product , preferably at least 75 mg / 100 ml of the functional food product ; and / or vitamin d in an amount of at least 0 . 5 μg / 100 ml of the functional food product , preferably at least 0 . 75 μg / 100 ml of the functional food product ; and / or folic acid in an amount of at least 75 μg / 100 ml of the functional food product , preferably at least 125 μg / 100 ml of the functional food product ; and / or vitamin e in an amount of at least 1 mg / 100 ml of the functional food product , preferably 1 . 5 mg / 100 ml of the functional food product ; and / or xantophylls in an amount of at least 250 μg / 100 ml of the functional food product , preferably at least 350 μg / 100 g egg yolk ; and / or iodine in an amount of at least 30 μg / 100 ml of the functional food product , preferably at least 45 μg / 100 ml of the functional food product ; and / or selenium in an amount of at least 5 μg / 100 ml of the functional food product , preferably at least 7 . 5 μg / 100 ml of the functional food product ; and / or zinc in an amount of at least 1 . 5 mg / 100 ml , preferably 2 . 5 mg / 100 ml . in an embodiment of the invention , said functional food product thus contains carbohydrates such as mono -, di - and / or trisaccharide and oligo and / or poly - saccharides . preferably said functional food products contains one or more mono -, di - and / or trisaccharides selected from the group of glucose , fructose , maltose , sucrose , galactose and lactose , in an amount ranging from 0 . 5 - 10 wt %, based on the total weight of the product , more preferably 1 - 7 . 5 wt %, most preferably 2 - 6 wt %. also in a preferred embodiment of the invention the functional food product contains one or more fibers , preferably selected from the group of ionic non - starch polysaccharides , including alginates , pectins ( including amidated pectins ), carrageenans , xanthans , gellans , furcellarans , karaya gum , rhamsan , welan , gum ghatti , gum arabic and salts ; neutral non - starch polysaccharides , including galactamannan , guar gum , locust bean gum , tara gum , ispaghula (= psyllium ), beta - glucans , konjac mannans , methylcellulose , gum tragacanth , detarium , tamarind , as well as fructo - oligosaccharides ( fos ), galacto - oligosaccharides ( gos ), inulin , celluloses , chemically - modified celluloses and vegetable and / or fruit fibres . preferably the amount of fibers contained in the food product is within the range of 0 . 1 - 20 wt %, more preferably within the range of 0 . 2 - 10 wt %, most preferably within the range of 0 . 5 - 7 . 5 wt %. furthermore , in an embodiment of the invention , the functional food product contains proteins and / or peptides other than the egg yolk protein , preferably selected from the group of dairy proteins ; vegetable proteins ; meat derived proteins and fish derived proteins as well as partial hydrolysates thereof , especially from the group consisting of whey protein , caseinates , soy protein , pea protein , maize protein , wheat protein , gelatin , albumin , their partial hydrolysates and combinations thereof , in an amount ranging from 0 . 1 - 20 wt %, based on the total weight of the product , more preferably 0 . 2 - 10 wt %, most preferably 0 . 5 - 7 . 5 wt %. in one preferred embodiment the functional food product contains less than 5 wt % of egg white albumin , more preferably less than 3 . 5 wt %, still more preferably less than 2 . 0 wt %, and most preferably less than 1 . 0 wt %. furthermore , in an embodiment of the invention , the functional food product contains lipid materials other than the egg yolk lipids , preferably selected from the group of milk fat , butter fat and vegetable oils , animal fats and fish oils preferably from the group of milk fat and butter fat , in an amount ranging from 0 . 05 - 7 . 5 ″\\ 1 .%, based on the total weight of the product , more preferably 0 . 1 - 5 wt %, most preferably 1 . 5 - 3 ″\\ 1 .%. in addition , the functional food product of the invention may comprise additional ingredients such as colouring agents , flavourings , preservatives , anti - oxidants , viscosity modifying agents , emulsifiers , food acids and the like , which will be applied in conventional amounts known to the skilled person . in accordance with a particularly preferred embodiment of the invention , the functional food product comprises little or no ethanol . preferably the amount of ethanol in the functional food product is below 5 wt %, based on the total weight of the functional food product , more preferably it is below 1 wt %, still more preferably it is below 0 . 5 wt %. most preferably the functional food product does not contain any ethanol . another aspect of the invention concerns a functional food product as described herein before , for use as a medicament . as explained herein before , each of the pharmacologically active nutrients of the invention has been implicated in a variety of conditions and disorders . in a preferred embodiment of the invention the at least one pharmacologically active nutrient is omega - 3 fatty acid and the functional food products is intended for reducing the incidence and / or severity of amd , cardiovascular disease , stroke , adhd , alzheimer &# 39 ; s disease or depression . in another embodiment of the invention the at least one pharmacologically active nutrient is vitamin d and the functional food products is intended for reducing the incidence and / or severity of bone softening diseases , osteoporosis or vitamin d deficiency . in another embodiment of the invention the at least one pharmacologically active nutrient is vitamin e and the functional food products is intended for reducing the incidence and / or severity of cardiovascular disease , cataract growth , age - related macular degeneration , glaucomatous damage of the retina , alzheimer &# 39 ; s disease , parkinson &# 39 ; s disease or vitamin e deficiency . in another embodiment of the invention the at least one pharmacologically active nutrient is folic acid and the functional food products is intended for reducing incidence and / or severity of cardiovascular disease , depression or decreases in short - term memory , mental agility and / or verbal fluency . in another embodiment of the invention the at least one pharmacologically active nutrient is a xanthophyll , especially lutein or zeaxanthine , and the functional food products is intended for reducing the incidence and / or severity of age - related macular degeneration , cataract , atherosclerosis and / or for improving skin health . in another embodiment of the invention the at least one pharmacologically active nutrient is iodine and the functional food products intended for reducing the incidence and / or severity of hypothyroidism or iodine deficiency . finally , in another embodiment of the invention the at least one pharmacologically active nutrient is selenium and the functional food products is intended for reducing the incidence and / or severity of selenium deficiency . yet another aspect of the invention concerns a method of treating or preventing any of the conditions or disorders described here above in a subject by administering to said subject a sufficient amount of the functional food product , wherein the at least one pharmacologically active nutrient of the invention is the one implicated in said condition or disorder . another aspect of the invention concerns the use of egg yolk that is obtained by feeding poultry a diet enriched in at least one pharmacologically active nutrient , preferably a pharmacologically active nutrient selected from the group consisting of omega - 3 fatty acids , especially dha and epa ; vitamin d ; folic acid ; vitamin e ; xantophylls , especially lutein and zeaxanthin ; iodine ; selenium ; and zinc , collecting the eggs produced by said poultry and taking therefrom the yolk , for the manufacture of a functional food product , essentially in accordance with what has been described herein before . in a preferred embodiment , the invention provides the use as defined here above , wherein the functional food product is a product for use in a method of treating and / or preventing a disorder or condition benefiting from supplementation of one or more of omega - 3 fatty acids , especially dha and epa ; vitamin d ; folic acid ; vitamin e ; xantophylls , especially lutein and zeaxanthin ; iodine ; selenium ; and zinc . yet another aspect of the present invention concerns a combination of egg yolk solids and dairy solids , especially buttermilk solids . as will be understood by the skilled person , on the basis of the information provided in this application , and in particular from the examples described below , such a combination constitutes a very suitable matrix for the ( frequent ) oral administration of pharmacologically active substances to a subject , especially fat soluble pharmacologically active substances . such a matrix affords high biological availability of said pharmacologically active substances , typically owing to certain egg yolk constituents , without the concomitant adverse effects on plasma cholesterol levels normally observed as a consequence of egg yolk consumption . a particularly suitable matrix in accordance with this aspect of the invention comprises at least 10 wt %, on the basis of dry solids weight , of a combination of egg yolk solids and dairy solids , more preferably 15 - 99 . 9 . wt %, most preferably 25 - 95 wt %. said combination typically contains the egg yolk solids and the dairy solids in a ratio within the range of 0 . 1 - 100 , preferably 1 - 10 , most preferably 2 . 5 - 10 . in a particularly preferred embodiment of the invention said egg yolk solids are selected from egg yolk phospholipids . in another embodiment the combination comprises whole egg yolk solids . it is furthermore particularly preferred that said dairy solids are selected from buttermilk solids , skimmed milk solids , semi - skimmed milk solids , fermented milk solids , especially yoghurt solids , fractions thereof and mixtures thereof , most preferably from buttermilk solids and fractions thereof . in an embodiment of the invention , the combination comprises whole buttermilk solids . in another embodiment of the invention the combination comprises one or more polar dairy lipids , preferably selected from the group of phospholipids and sphingolipids , most preferably from sphingolipids . preferably said lipids are present in an amount of , based on the total weight of the product , at least 0 . 05 wt %, more preferably at least 0 . 075 wt %. another preferred embodiment of the invention concerns the combination defined before , further comprising an effective amount , e . g . 10 4 c : fu / ml or more , preferably 10 5 c : fu / ml or more , most preferably 10 6 c : fu / ml or more , of lactic acid producing bacteria , more preferably of bacteria selected from lactobacillus or lactococcus , more preferably from l . lactis , l . acidophilus , l . casei , l . reuteri , l . gassert and l . delbrueckii , most preferably from l . acidophilus , l . delbrueckii and l . casei . preferably said combination comprises at least one pharmacologically active substance , preferably at least one fat soluble ( or lipophilic ) pharmacologically active substance , typically in an amount of at least 0 . 01 wt %, more preferably 0 . 05 - 10 wt %, most preferably 0 . 1 - 5 wt %. the above - described combinations of egg yolk solids and buttermilk solids are particularly suitable i ) for use as a matrix for the oral administration of one or more lipophilic pharmacologically active substance ; ii ) for increasing the oral bio - availability of one or more lipophilic pharmacologically active substances ; iii ) for use in a method of treating a subject in need thereof , said method comprising the administration of a lipophilic pharmacologically active substance dispersed in said combination of egg yolk solids and buttermilk solids ; and / or iv ) for use in the manufacture of a pharmaceutical or nutraceutical formulation for improving the oral bio - availability of lipophilic pharmacologically active substances . these uses as such as well as the above - described combinations presented or packaged for these uses are also encompassed by the scope of the present invention . example 1 : production of egg yolk enriched in lutein , zeaxanthin or omega - 3 fatty acids feeds for producing the enriched eggs were formulated and produced within the legal requirements for animal feed . the use of lutein and zeaxanthin is regulated under eu regulation 1831 / 2003 . the dosage of lutein and zeaxanthin in feed did not exceed the legal limit of 80 ppm in animal feed . for omega 3 fatty acids is a component of some ingredients like linseed meal and fish oil . these products are not limited for use as feed ingredient . testing showed the following concentrations for eggs produced by poultry fed the lutein or zeaxanthin enriched feed : the omega - 3 concentration in the eggs produced by poultry fed the omega - 3 enriched feed is approximately 200 ± 10 mg . two types of products have been prepared containing egg yolk as produced in accordance with example 1 . the first product was a 100 ml drink which is intended for daily use ( a so - called “ shot ”). the composition of different types of such drinks is given in table 1 below . the second product prepared in this example was a 150 ml dessert ( a pudding ) which is intended for daily use . the composition of the dessert is given in table 2 below . a randomized placebo - controlled trial is set up using eggs and product produced herefrom in accordance with the present invention , said eggs being enriched in lutein , zeaxanthin and omega 3 fatty acids . the group of test - subjects is divided into 5 groups , consuming either no eggs , normal eggs ( lutein : 0 . 168 ± 0 . 08 , zeaxanthin 0 085 ± 0 . 0017 ), eggs enriched with lutein ( lutein : 0 . 921 ± 0 . 106 , zeaxanthin : 0 . 137 ± 0 . 014 ), eggs enriched with zeaxanthin ( lutein : 0 . 174 ± 0 . 014 , zeaxanthin : 0 . 487 ± 0 . 031 ), or egg product made from the lutein enriched eggs , respectively . the omega - 3 concentration is the same for all eggs and is 200 ± 10 mg . boiled eggs were prepared and packed in board or plastic containers and distributed to the test subjects . also a buttermilk drink containing egg yolk of the enriched eggs was prepared and packed in 100 ml bottles and distributed to the test subjects . the butter - milk drink had the same composition as product no . 2 of example 2 . the expiration time for eggs is 28 days . by using boiled eggs stored in a refrigerator this shelf live can be extended to 8 weeks . the buttermilk product , which is pasteurized , tested for cooled shelf live for a period of 6 weeks , and 4 weeks at room temperature . each test - subject is asked to consume one egg or egg product daily for a period of 90 days . these are either modified or control eggs or a butter - milk drink in accordance with the invention or a control butter - milk drink product . the products are consumed at lunch time . during this period the subjects are asked to fill in questionnaires which are aimed at establishing the willingness of the subject to comply with the treatment regimen , i . e . the willingness to take the egg or the product of the invention on a daily basis . it is especially aimed to establish whether subjects developed disinterest or dislike towards the product . the study includes follow up time after the 3 months actual trial such that the total study time will be 2 years . during the 3 month period there are 3 measuring point at predetermined intervals . at every measuring point ( days 1 , 45 and 90 ) the subjects undergo invasive and non - invasive measuring techniques . for the invasive part of the study blood samples were taken , and the serum levels of cholesterol , lutein , zeaxanthin , triglycerides and lipoproteins were determined . table 3 shows the descriptives of 5 groups . in total there were 96 subjects which completed the whole study . there were no statistical significant deferences between the groups for both age ( p = 0 . 434 ) and gender ( p = 0 . 993 ). for the lipids three groups were compared , namely the control group , beverage group , and the eggs - group — normal , lutein , and zeaxanthin eggs — together . an anova analysis revealed no difference in total cholesterol ( p = 0 . 951 ), ldl cholesterol ( p = 0 . 969 ), hdl cholesterol ( p = 0 . 877 ), or triglycerides ( p = 0 . 542 ) at baseline ( see table 2 ). analysis of the effect of egg consumption on the total cholesterol levels showed no significant change in total cholesterol between baseline and endpoint between the three groups ( p = 0 . 163 ). stratification for gender did however showed a significant effect in the female population ( p = 0 . 018 ). an anova comparison showed no significant change in ldl between the three groups ( p = 0 . 300 ), stratification for gender showed a significant contribution of eggs in the female population ( p = 0 . 025 ). analysis of changes in plasma hdl concentration revealed no significant change between the groups ( p = 0 . 257 ). there was no significant difference in triglycerides change in between the three groups ( p = 0 . 170 ). at baseline there was a statistically significant higher plasma lever of lutein in the lutein egg group as compared to the lutein beverage group ( p = 0 . 036 ) but not with to the control group ( p = 0 . 058 , table 3 ). stratification for gender resulted in no significant difference at baseline . for zeaxanthin there was no difference between the 5 groups there was a significant change in plasma lutein level between baseline and end point for both the lutein beverage as the lutein egg group when compared to the control group ( p & lt ; 0 . 001 in both case ). a repeated measurement model showed the same results with p & lt ; 0 . 001 for lutein beverage and lutein egg group when compared to the control group . a mixed model analysis of the measurement with subject number as grouping factor , plasma lutein levels as dependant , diets as factors and week as covariate revealed a significant effect of week and lutein beverage and lutein egg group as compared to the control group ( p & lt ; 0 . 001 ), the zeaxanthin group did not reach significance ( p = 0 . 080 ). at baseline there was no difference in plasma levels of zeaxanthin between the 5 groups ( p = 0 . 482 ). the change after 90 days was significantly different for the zeaxanthin egg group when compared to the control group ( p & lt ; 0 . 001 ). a repeated measurement model resulted in a significant difference for both the as the zeaxanthin egg when compared to the control group ( p & lt ; 0 . 001 respectively ). a mixed model analysis of the measurement with subject number as grouping factor , plasma zeaxanthin levels as dependant , diets as factors and week as covariate revealed a significant effect of week and all diets as compared to the control group , the normal egg group ( p = 0 . 050 ), lutein beverage ( p = 0 . 042 ), the lutein egg ( p = 0 . 006 ), and the zeaxanthin egg group ( p & lt ; 0 . 001 ). the complete results of the serum analyses of the subjects of the 5 test groups is given in the following tables 4 and 5 for the non - invasive part of the study the subjects underwent the following tests : mean visual acuity test using the early treatment diabetic retinopathy study ( etdrs )— chart , contrast sensitivity using the pelli - robson chart , scanning laser ophthalmoscope ( slo ), optical coherence tomography ( oct ), mpod using heterochromatic flicker photometry ( hfp ), macular photocoagulation study ( mps ) and macular pigment reflectometry ( mpr ). at baseline there were no significant difference between the 5 groups with any of the measuring devices ( p & gt ; 0 . 356 ). however , a repeated measurement analysis shows a significant contribution of both the normal egg as the lutein egg diet for the mpr - lutein measurement in the female population ( p = 0 . 025 and p = 0 . 041 respectively ). a mixed model analysis of the measurement with subject number as grouping factor , mps as dependant , diets as factors and week as covariate showed no significant effect of week and diets as compared to the control group ( p & gt ; 0 . 237 ). at mid point there was a significant difference between the lutein egg group and control group for the mpr - lutein measurement ( p = 0 . 046 ) but this disappeared by end point . stratification for gender showed the difference at midpoint for the mpr - lutein to be in the female population ( p = 0 . 023 ). a mixed model analysis of the measurement with subject number as grouping factor , mpr lutein - mpod as dependant , diets as factors and week as covariate revealed a significant effect of week and lutein egg group as compared to the control group ( p = 0 . 006 ). stratification for gender showed a significant effect of week and lutein beverage ( p = 0 . 039 ) on lutein fraction of the mpod compared to the control group in the male population . the lutein egg group did not reach significance ( p = 0 . 109 ). in the female population there were statically significant changes in both the lutein beverage and lutein egg group as compared to the control group ( p = 0 . 024 and p = 0 . 016 respectively ). a mixed model analysis of the measurement with subject number as grouping factor , mpr zeaxanthin - mpod as dependant , diets as factors and week as covariate revealed no significant effect of week and diets as compared to the control group ( p & gt ; 0 . 291 ). stratification for gender showed a significant effect of week and lutein beverage ( p = 0 . 007 ) on zeaxanthin fraction of mpod compared to the control group in the male population . the normal egg group and lutein egg group both reached significance ( p = 0 . 031 and p = 0 . 026 respectively ) in the female population . a mixed model analysis of the measurement with subject number as grouping factor , mpr - mpod as dependant , diets as factors and week as covariate revealed no significant effect of week and diets as compared to the control group ( p = 0 . 091 ). stratification for gender showed a significant effect of week and lutein beverage ( p = 0 . 003 ) on mpod as compared to the control group in the male population . in the female population there were also significant changes between the normal egg group and lutein egg group as compared to the control group ( p = 0 . 032 and p = 0 . 015 respectively ). a mixed model analysis of the measurement with subject number as grouping factor , slo as dependant , diets as factors and week as covariate revealed a significant effect of week and zeaxanthin group compared to the control group ( p = 0 . 009 ), the lutein group did not reach significance ( p = 0 . 057 ). stratification for gender showed a significant effect of week and lutein beverage ( p = 0 . 013 ), lutein egg ( p = 0 . 036 ), and zeaxanthin ( p = 0 . 007 ) egg on mpod compared to the control group in the female population . there were no significant changes in the male population . comprehensive results of the non - invasive analyses of the subjects of the 5 test groups is given in the following table 6 . the results of the first 3 month trial period show that the product of the invention is a suitable vehicle for delivering lutein and zeaxanthin , as evidenced by the results of the analyses of the blood samples . furthermore , it was established that the level of macular pigment increased throughout the study . the current belief is that lutein and zeaxanthin accumulated in the macular region can help in the prevention of amd by absorbing this blue light and protecting the retina from oxidative stress by neutralizing free radicals . the possible protective nature of omega - 3 is believed to work by regulating inflammatory and immune response in the retina , repairing damaged cells , and improving endothelial cell function . furthermore , the clinical trial results suggest that compliance by subjects taking the product of the present invention will be significantly higher than that by subjects taking eggs , when the regimen comprises daily intake for periods of several months to even years . finally , the clinical trial results show that the daily intake of an egg yolk product containing buttermilk did not give rise to significant adverse changes in plasma cholesterol levels , triglyceride levels and lipoproteins .
the present invention relates to the field of food products , especially food products specifically designed for providing health benefits beyond the basic function of nutrition for a subject consuming the product on a regular basis , e . g . on a daily basis . according to the present invention eggs are produced , in particular chicken eggs , that are enriched in one or more pharmacologically active nutrients . the egg yolk obtained from the aforementioned eggs can suitably be used in high amounts as a component of a functional food product having all the characteristics that are normally required for such functional food products . a preferred example of a food product in accordance with the invention is a ready to drink shot containing approximately one whole egg yolk in a volume as small as 100 ml .