the present invention relates to anthelmintic active compound combinations including avermectins , hexahydropyrazinoisoquinolines and anthelmintic pyrimidines such as tetrahydropyrimidines . acceptable tetrahydropyrimidines include , for example , pyrantel , morantel and oxantel . acceptable hexahydropyrazinoisoquinolines include , for example , praziquantel . other acceptable actives include benzazepines and salicylamides . acceptable avermectins include , for example , ivermectin , doramectin , selamectin and abamectin . a formulation of active ingredients comprising ivermectin , praziquantel and pyrantel is particularly preferred . the active ingredients target different pathogenic organisms that can adversely affect the health of a mammal . this particular combination is particularly effective in fighting a wide variety of organisms . however , administering three physically separate pharmaceutical compositions to an animal is undesirable and it has been determined that it would be beneficial to combine the ingredients into one formulation , in particular one tablet ( or capsule ) containing a pharmaceutically effective amount of the active ingredients , thereby decreasing the number of administrations of formulations to the animal . the disease or group of diseases described generally as helminthiasis is due to infestation of an animal host with parasitic worms known as helminths . helminthiasis is a prevalent and serious economic problem in domesticated animals such as swine , sheep , horses , cattle , goats , dogs , cats and poultry . among the helminths , the group of worms described as nematodes causes widespread and often times serious infection in various species of animals . still other parasites may be located in other tissues and organs of the body such as the heart and blood vessels , subcutaneous and lymphatic tissue and the like . the parasitic infections known as helminthiases lead to anemia , malnutrition , weakness , weight loss , severe damage to the walls of the intestinal tract and other tissues and organs and , if left untreated , may result in death of the infected host . although the antiparasitic agents of this invention find their primary use in the treatment and / or prevention of helminthiasis , they are also useful in the prevention and treatment of diseases caused by other parasites , for example , arthropod parasites such as ticks , lice , fleas , mites and other biting insects in domesticated animals and poultry . repeat treatments are given as required to combat re - infestations and are dependent upon the species of parasite . the techniques for administering these materials to animals are known to those skilled in the veterinary field . the preparations are suitable for combating pathogenic endoparasites which occur in animal husbandry and animal breeding in productive , breeding , zoo , laboratory , experimental animals and pets , and have a favorable toxicity to warm - blooded animals . in this connection , they are active against all or individual stages of development of the pests and against resistant and normally sensitive species . by combating pathogenic endoparasites , it is intended that disease , cases of death and reduction in production ( for example in the production of meat , milk , wool , hides , eggs , etc .) are reduced so that more economic and simpler animal husbandry is possible by means of the use of the pharmaceutical formulation . the productive and breeding animals include mammals , such as , for example , cattle , horses , sheep , pigs , goats , camels , water buffalo , donkeys , rabbits , fallow deer and reindeer , pelt animals , such as , for example , mink , chinchilla and raccoons , birds , such as , for example , chickens , geese , turkeys and ducks , fresh and salt - water fish , such as , for example , trout , carp and eels , and reptiles . the laboratory and experimental animals include mice , rats , guinea pigs , golden hamsters , dogs and cats . the formulation according to the invention is particularly preferably administered to dogs and cats , but is suitable for other mammals . the formulations can be administered directly or in the form of suitable preparations , enterally , parenterally or dermally . enteral administration of the formulations takes place , for example , orally in the form of powder , tablets , capsules , pastes , potions , granules , orally administered solutions , suspensions and emulsions , boli , medicated feed or drinking water . oral solutions and concentrates for oral administration after dilution ; emulsions and suspension for oral administration ; and semisolid preparations ; formulations in which the active compound is processed in an ointment base or in an oil - in - water or water - in - oil emulsion base ; solid preparations , such as powders , premixes or concentrates , granules , pellets , tablets , boli and capsules , with tablets the preferred form ; oral solutions are prepared by dissolving the active compound in a suitable solvent and , if appropriate , adding additives such as solubilizers , acids , bases , buffer salts , antioxidants and preservatives . the solutions are filtered and packed under sterile conditions . solvents may include : physiologically acceptable solvents , such as water , alcohols , such as ethanol , butanol , benzyl alcohol , glycerol , propylene glycol and polyethylene glycol , n - methylpyrrolidone , and mixtures of the same . the active compounds can , if appropriate , also be dissolved in physiologically acceptable vegetable or synthetic oils . solubilizers may include : solvents which promote dissolution of the active compound in the main solvent or substances which prevent precipitation of the active compound . examples are polyvinyl pyrrolidone , polyoxyethylated castor oil and polyoxyethylated sorbitan esters . one particularly preferred formulation of the invention , comprising three active ingredients , is preferably administered in the form of capsules , more preferably tablets . a preferred formulation of the present invention contains 0 . 005 - 25 % ivermectin , preferably 0 . 01 - 15 %, and most preferably 0 . 012 - 5 %, with 0 . 016 % as a preferred example . a preferred formulation of the present invention can contain 2 . 0 - 58 % of a secondary anthelmintic drug , such as an isoquinoline , preferably praziquantel , preferably 6 - 41 %, and most preferably 11 . 2 - 23 %, with 13 . 6 % as a preferred example . a preferred formulation of the present invention can contain 1 . 5 - 76 % of an anthelmintic pyrimidine , preferably pyrantel , preferably 6 - 52 %, and most preferably 11 . 2 - 23 %, with 13 . 6 % as a preferred example . all percentages herein , unless otherwise evident , are on a weight basis . a preferred dosage of avermectin , e . g ., ivermectin , is about 5 - 7 μg / kg body weight of the animal administered monthly , preferably 5 . 5 - 6 . 5 μg / kg body weight , with 6 μg / kg body weight as a preferred example . a preferred dosage of anthelmintic pyrimidines , e . g ., pyrantel , is about 4 . 25 - 5 . 75 mg / kg body weight administered monthly , preferably 4 . 75 - 5 . 25 mg / kg , with 5 mg as a preferred example . a preferred dosage of hexahydropyrazinoisoquinaline , e . g ., praziquantel , is about 4 . 25 - 5 . 75 mg / kg body weight administered monthly , preferably 4 . 75 - 5 . 25 mg / kg , with 5 mg as a preferred example . to prepare solid preparations , the active compound should be mixed with suitable excipients , if appropriate , with addition of auxiliaries , and converted to the form desired . ( a ) preparing a first , or a first and second , or a first , second and third combination including the first , or the first and second , or the first , second and third active ingredient , respectively , or of course , formulations involving more than three active ingredients ; ( b ) combining the combinations from ( a ) with dispersing agents comprising carrier material to form one , two or three separate aqueous solutions ; ( c ) granulating one or all of the solutions , preferably by spray granulation , from ( b ) ( d ) drying the resulting granules , if needed ; ( e ) blending the granules from ( d ), which contain the first , second and third active ingredients and carrier material ; and ( f ) if desired , forming the blended granules into tablets or filling a capsule . spray granulation involves the drying of liquid ( i . e ., solution , suspension melt and so forth ) while simultaneously building particle size . by mixing an active ingredient with a carrier in the liquid phase , the active can become “ encapsulated ” or substantially covered in a matrix of carrier after the spray granulation process . granulation is generally performed by spraying liquid into the fluidized powder . the granules are subsequently dried with heated air . suitable excipients may include physiologically acceptable inert solids such as , for example , sodium chloride , calcium carbonate , hydrogen carbonates , aluminum oxides , silicas , clays , precipitated or colloidal silicon dioxide and phosphates . other suitable excipients may include , for example , sugar , cellulose , croscarmellose sodium , aerosil , nutrients and feedstuffs , such as milk powder and pork liver powder , animal meals , ground and crushed cereal meals , avicel ph102 and starches . auxiliaries can include preservatives , antioxidants and colorants . additional suitable auxiliaries can include lubricants , such as , for example , magnesium stearate , stearic acid , talcum and bentonites , disintegration - promoting substances , such as starch or transversely crosslinked polyvinyl pyrrolidone , binders , such as , for example , starch , gelatin or linear polyvinyl pyrrolidone , and dry binders , such as microcrystalline cellulose . the formulation can also be in the form of a chewable , such as a beef - chewable containing ground or minced beef or other meat , in addition to other excipients listed above . the materials in the final formulation , such as the excipients , auxiliaries , synergists and other materials , which aid in delivery , shelf - life , desired physical structure and so forth will be referred to herein generally as carrier material . as stated herein , carrier material could be pharmaceutically active under certain circumstances . the following example is given for purposes of illustration only and is not intended to be construed in a limiting manner . the following materials ( in the order listed below ) were passed through a russel sieve fitted with 20 # sieve and collected in a stainless steel drum : the delumped material resulting from the step above was added to the drum tumbler and blended for 20 minutes . purified water was added to a stock pot with citric acid and sodium citrate dihydrate . the contents were mixed for 5 minutes with a stirring rod . polyethylene glycol flakes were added to a separate stock pot and heated with a water bath to a temperature of 45 ± 5 ° c . to melt the flakes . the solution was maintained at this temperature . ivermectin was added to the melted polyethylene glycol with gentle stirring until the compound was dissolved . the solution was maintained at 45 ± 5 ° c . 161 . 5 g of the citrate buffer detailed above was added to the melted polyethylene glycol / ivermectin solution and stirred with gentle agitation for at least 5 minutes until the solution was clear . the stirring was then ceased to allow any air bubbles to escape and the solution was maintained at 45 ± 5 ° c . the remaining citrate buffer solution was placed on a hot plate and heated to a temperature of 55 ± 5 ° c . the blended avicel , croscarmellose sodium and povidone was transferred to a spray granulator . the solutions were spray granulated as follows : ( 1 ) inlet air temperature : 50 ± 10 ° c . ( 2 ) outlet air temperature : 45 ± 10 ° c . ( 3 ) bed temperature : 43 ± 10 ° c . ( 4 ) atomization pressure : 3 - 5 bar ( 5 ) spray rate : 100 g ± 20 g per minute ( 6 ) pan speed : 2 - 10 rpm ( a ) the ivermectin / polyethylene glycol / citrate buffer solutions was sprayed at a rate of 100 ± 20 g / minute until all of the solution was sprayed . ( b ) the reserve citrate buffer at 55 ± 5 ° c . was added to the container which held the previous solution for rinsing purposes . the rinse citrate buffer was sprayed at a rate of 100 ± 20 g / minute . ( c ) granulation was continued by spraying 300 g of purified water at room temperature . additional purified water was spayed until the desired consistency was achieved . the granules were then emptied into the drying bowl and dried using a fluid bed drier . after drying , the bowl was removed and the granules were mixed with a scoop . the dried granules obtained were transferred in double polythene lined suitable container . the sieved material was added to a diosna mixer and blended for 10 minutes using the impeller on low speed with the chopper off . the mixture was granulated with 9 , 000 g of purified water with the impeller and the chopper set on low speed . additional purified water was added to achieve the good granular mass . the granulated mixture was dried using a fluid bed drier and transferred to a double polythene lined suitable container . the following materials ( in the order listed below ) were passed through a russel sieve fitted with 20 # sieve and collected in a stainless steel drum : ( a ) praziquantel usp ( b ) povidone ( c ) croscarmellose sodium ( d ) avicel ph102 the delumped material was mixed in a drum tumbler for 20 minutes . the mixture was added to a diosna mixer and 10 l of purified water was gradually added with the impeller on low speed with the chopper activated for 5 minutes . the choppers were set on fast speed and run for 3 minutes . the granules were dried in a fluid bed drier and transferred to a double polythene lined suitable container . 13 , 658 g of purified water was added to a stock pot and mixed with medium agitation . citric acid and eudragit e - 100 was added to the stock pot . the mixture was stirred with medium agitation until the components had completely dissolved . the resulting eudragit solution was allowed to settle until the air bubbles had escaped . the praziquantel granulated mixture was added to the spray granulator and coated with the eudragit e - 100 solution . the resulting material was transferred to a double polythene lined suitable container . the first four excipients were sifted through a 500 # sieve and collected in a suitable container . then the magnesium stearate was sifted through a 500 # mesh sieve . the three mixtures containing the active ingredients of the formulation ( i . e ., mixtures a , b and c ) and the excipient mixture were blended in a drum tumbler for 25 minutes . the sifted magnesium stearate was added and blended for an additional 5 minutes . the formulation was then compressed into a plain round concave tablets of 420 mg or 840 mg and caplets of 1680 mg . it will thus be seen that the objects set forth above , among those made apparent from the preceding description , are efficiently attained and , since certain changes may be made in carrying out the above method and in the composition set forth without departing from the spirit and scope of the invention , it is intended that all matter contained in the above description shall be interpreted as illustrative and not in a limiting sense . it is also to be understood that the following claims are intended to cover all of the generic and specific features of the invention herein described and all statements of the scope of the invention which , as a matter of language , might be said to fall therebetween . particularly it is to be understood that in said claims , ingredients or compounds recited in the singular are intended to include compatible mixtures of such ingredients wherever the sense permits .
the present invention provides a method for preparing a pharmaceutical formulation containing ivermectin and a method and composition that can contain ivermectin plus hexahydropyrazinoisoquinolines and tetrahydropyrimidines . examples of these include praziquantel and pyrantel . a pharmaceutical formulation is provided for use in the treatment of helminthiasis of mammals , and particularly tapeworm , hookworm , roundworm and heartworm of domestic animals and farm animals . the present invention also provides a method of treating helminthiasis in mammals , which method comprises administering to the mammal in need thereof an anthelmintically effective amount of a pharmaceutical formulation of the invention .