What are the subjects that one must learn while an undergraduate to pursue an academic career in Biophysics? I am an undergraduate student pursuing a B.Sc(Hons.) degree in Physics. I want to pursue an academic career in Biophysics. So, I wanted to know which subjects are required for higher studies in Biophysics. In my course, we get to choose one ancillary subject for the first two semesters, then another ancillary subject for the next two semesters. The final two semesters are fully focused on Physics. I am about to complete my second semester, and I took up Mathematics as my ancillary subject. Now, I need to choose between Chemistry, Computer Science or Microbiology for the next two semesters, and I am not sure which one to choose. Microbiology is looking like the obvious choice but I think that knowledge of Chemistry and Computer Science is also going to have applications in Biophysics. So could you help me out by telling me which one of these subjects would be most beneficial in learning, keeping in mind that I want to pursue Biophysics in the future?
Specific biological domain knowledge will be something that you will learn in the course of doing research, and can be hard to predict at this point in your studies, because you have no idea what kind of graduate program you'll be admitted to or the specific research field of your prospective advisor. 

Therefore, while all of those directions sound fine and won't be wasted if you choose them, I would lean towards the more foundational topics, i.e. Chemistry or Computer Science, that you could use no matter what direction you took in the future. Another option would be statistics, since nearly any biophysics research involving experiments will need a very solid understanding of stats to design experiments with high statistical power, and to make sense of the resulting data without getting tricked by spurious correlations.