Using the same control pictures/data in two different papers :-)
We would like to ask a question of general interest, as it might be the case in other studies. It is the first time I stumble upon this situation.

In our research, we have used the wild-type of a microbial strain and mutants for about 7 distinct genes. They were all subjected to different treatments and assessed by the same response variables. The seven genes, however, are not strictly related to each other, so that 3 of them as a group make-up one story, and the other 4 make-up a different one (thereby, two distinct manuscripts). The reason why they were all worked together (the wild-type and the mutants for these genes) were simply logistics and efficiency of resources usage.

One of the authors are concerned with the fact that for both papers with different groups of mutants (hence very different stories), the wild-type controls were the same!  Therefore, he is afraid that using the same pictures and data in this case would configure a self-plagiarism issue between the two manuscripts.

Could you please help us on deciding whether this will indeed be an issue, or not?

Thank you very much in advance for your kindness and attention to this matter.

Best regards,
Dr. Leandro.
This is a recurrent issue in biological sciences, and seeking for peer advice is a commendable step. Most colleagues would just move on while trying to conceal the fact that the results of two different manuscripts have a significant overlap.

My general advice is quite simple: focus on the scientific outcome of your research and aim for robustness. Transparency is essential, so that others judge whether they can trust you. Give your readers &amp; peers all they need to follow your steps and rationale.

In short: as long as you explicitly declare the overlap and you unbiasedly trust it cannot affect your conclusions on both papers, I think you should be just fine.

Of course, independent controls and higher numbers of repetitions are always better for more representative results, and there will be always those who will not appreciate your "strategy". You cannot please everyone. Rest assured many of your critics include those who just don't like the concept of transparency.

Can you publish both papers in the same journal? Consider depositing all raw data in the same repository, to be linked to both papers.

Good luck!