Word limit for paper after addressing reviewer's comments After submitting a paper, receiving comments back from reviewers and revising, the paper manuscript may be longer than the given word limit due to the additional content added (even if it was at or below the word limit in the original submission). For the sake of argument, say the revised paper is 10% or 20% longer than the word limit.

Shortening the paper without loosing any information may be difficult. Moving some paragraphs into the Supplements may be possible, but may make it more difficult to find relevant information or be aesthetically displeasing, such as writing "Additional discussions can be found in the Supplements." in the middle of the text.

Are there any guidelines on how willing journals might be to accept papers above the word limit after revisions? Is word length still strictly enforced or is this mainly checked upon initial submission? Would simply submitting and hoping for a quick response from the editor (in case this is unacceptable) be a good strategy? Or is this a bad idea since either it highly unlikely to be acceptable or has a risk of annoying the editor? Do you have any experience with this issue either from an author's or an editor's point of view?
I can now provide an answer to my own question (I hope this is alright): I recently submitted a revised article that was about 15% longer than the word limit to a small biomedical journal. The original version of the article kept to the word limit. In the rebuttal letter, I explained that the article had become longer due to the reviewers' question. This article was accepted without further comment from the editor.

So it seems that at least some editors do not see a problems with longer articles, as long as the original submission keeps to the limit. I suspect that the rules may vary greatly between disciplines and journals and that no journal will ever explicitly state "we do not care about length for revised submissions". My PI was very confident that the article would be accepted, so it may be advisable to ask an experienced colleague in the field for their opinion.