Change of affiliation in manuscript under preparation I have submitted a paper to a journal as part of my degree at university X. This paper includes my correspondence e-mail and affiliation to the laboratory at university X, of which my co-authors were a part.

I have now moved to another university in a different country and the paper has been returned with the reviewers' comments. Should I be updating my affiliation and e-mail, especially considering that the majority of the work (the submitted draft) was completed at my old lab? I'm not sure if this is important at all, or whether it has any implications for the eventual transfer of copyright.
If you are doing the revision work at the new place you can just add the new affiliation as a second one (e.g. with 1,2).

Yes, I would update the email as there is no point in a dead old one for anybody.