Please translate the given English sentence to Hindi
इसलिए, एक बार जब आप (agarose) में अलग हो गए हैं, तो आप कुछ रसायनों के साथ इलाज करते हैं, उदाहरण के लिए सोडियम हाइड्रोक्साइड, जो डीएनए के दो किस्में के बीच सभी हाइड्रोजन बांड को तोड़ता है और अब आप डीएनए को झिल्ली में स्थानांतरित करते हैं, जो जेल के ऊपर रखा जाता है।
So, once you have separated in agarose, you treat with certain chemicals, for example sodium hydroxide, which breaks all the hydrogen bonds between the two strands of the DNA and now you transfer the DNA to the membrane, that is kept over the gel and what you do in this case is that, you have a support, wherein you have the gel and then on the top of that you put a membrane and you allow the salt solution to pass through the agar to over the membrane and to the paper towel that are kept on the top.