< 110 > Avila Therapeutics , Inc . < 120 > HCV protease inhibitors and uses thereof < 130 > 2007878 - 0052 < 150 > US 61 / 016 , 110 < 151 > 2007 - 12 - 21 < 150 > US 61 / 016 , 473 < 151 > 2007 - 12 - 23 < 150 > US 61 / 060 , 371 < 151 > 2008 - 06 - 10 < 150 > US 61 / 098 , 668 < 151 > 2008 - 09 - 19 < 160 > 77 < 170 > KopatentIn 1 . 71 < 210 > 1 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 1 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 2 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 2 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 3 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 3 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 4 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 4 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 5 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 5 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 6 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 6 Gly His Val Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 7 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 7 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 8 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 8 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 9 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 9 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 10 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 10 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Ser Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 11 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 11 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Ser Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 12 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 12 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Ser Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 13 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 13 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Ala Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 14 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 14 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Ala Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 15 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 15 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Ser Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 16 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 16 Ala His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Ser Arg Gly Val 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 17 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 17 Gly His Ala Val Gly Ile Phe Arg Ala Ala Ile Cys Thr Arg Gly Ala 1 5 10 15 Ala Lys Ser Ile 20 < 210 > 18 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 18 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 19 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 19 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 20 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 20 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 21 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 21 Gly His Val Met Gly Ile Phe Ile Ala Val Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 22 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 22 Gly His Val Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 23 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 23 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 24 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 24 Gly His Ala Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 25 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 25 Gly His Ala Ala Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 26 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 26 Gly His Ala Ala Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 27 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 27 Gly His Ala Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 28 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 28 Gly His Ala Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Thr Val 20 < 210 > 29 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 29 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 30 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 30 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 31 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 31 Gly His Val Val Gly Val Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 32 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 32 Gly His Val Val Gly Val Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 33 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 33 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 34 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 34 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 35 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 35 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 36 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 36 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 37 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 37 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 38 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 38 Asp His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 39 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 39 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 40 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 40 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 41 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 41 Gly His Ala Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 42 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 42 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 43 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 43 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Leu 20 < 210 > 44 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 44 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 45 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 45 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 46 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 46 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 47 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 47 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 48 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 48 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 49 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 49 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 50 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 50 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 51 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 51 Gly His Val Ala Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 52 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 52 Gly His Ala Val Gly Val Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 53 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 53 Gly His Ala Val Gly Val Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 54 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 54 Gly His Val Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 55 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 55 Gly His Val Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ser Leu 20 < 210 > 56 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 56 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 57 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 57 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 58 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 58 Gly His Ala Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 59 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 59 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 60 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 60 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 61 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 61 Gly His Val Val Gly Leu Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Ile 20 < 210 > 62 < 211 > 20 < 212 > PRT < 213 > Hepatitis C virus < 400 > 62 Ser His Cys Val Gly Ile Phe Arg Ala Ala Val Cys Thr Arg Gly Val 1 5 10 15 Ala Lys Ala Val 20 < 210 > 63 < 211 > 21 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 63 acgcagaaag cgtctagcca t 21 < 210 > 64 < 211 > 19 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 64 tactcaccgg ttccgcaga 19 < 210 > 65 < 211 > 23 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > 6 - carboxyfluorescein < 220 > < 221 > misc _ feature < 222 > ( 23 ) . .( 23 ) < 223 > C a rboxytetramethylrhodamine < 220 > < 221 > modified _ base < 222 > ( 23 ) . .( 23 ) < 400 > 65 cctggaggct gcacgacact cat 23 < 210 > 66 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 66 gctgtgggca tctttcggtc tgccgtgtgc acccgaggg 39 < 210 > 67 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 67 ccctcgggtg cacacggcag accgaaagat gcccacagc 39 < 210 > 68 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 68 gctgtgggca tctttcggac tgccgtgtgc acccgaggg 39 < 210 > 69 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 69 ccctcgggtg cacacggcag tccgaaagat gcccacagc 39 < 210 > 70 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 70 ggggttgcga aggcggtggc ctttgtaccc gtcgagtct 39 < 210 > 71 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 71 agactcgacg ggtacaaagg ccaccgcctt cgcaacccc 39 < 210 > 72 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 72 ggggttgcga aggcggtggt ctttgtaccc gtcgagtct 39 < 210 > 73 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 73 agactcgacg ggtacaaaga ccaccgcctt cgcaacccc 39 < 210 > 74 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 74 atctttcggg ctgccgtgag cacccgaggg gttgcgaag 39 < 210 > 75 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 75 cttcgcaacc cctcgggtgc tcacggcagc ccgaaagat 39 < 210 > 76 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 76 cacgctgtgg gcatctttaa ggctgccgtg tgcacccga 39 < 210 > 77 < 211 > 39 < 212 > DNA < 213 > Artificial sequence < 220 > < 223 > Synthetic ; designed from HCV protease NS 3 < 400 > 77 tcgggtgcac acggcagcct taaagatgcc cacagcgtg 39