SEQUENCE LISTING < 110 > Juno Therapeutics , Inc . CHRISTIN , Brian COVINGTON , Michael G DAVE , Kedar H GETTO , Richard J Jr . KOWSKI , Tom LARSON , Ryan P RAMSBORG , Christopher G TREDE , Nikolaus S WEBER , Clinton WHITMORE , James B YEE , Nathan < 120 > COMPOSITIONS , ARTICLES OF MANUFACTURE AND METHODS RELATED TO DOSING IN CELL THERAPY < 130 > 735042009340 < 150 > 62 / 464 , 371 < 151 > 2017 - 02 - 27 < 150 > 62 / 465 , 817 < 151 > 2017 - 03 - 01 < 150 > 62 / 470 , 180 < 151 > 2017 - 03 - 10 < 150 > 62 / 527 , 002 < 151 > 2017 - 06 - 29 < 150 > 62 / 580 , 416 < 151 > 2017 - 11 - 01 < 150 > 62 / 584 , 740 < 151 > 2017 - 11 - 10 < 150 > 62 / 596 , 703 < 151 > 2017 - 12 - 08 < 160 > 58 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 12 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > spacer ( IgG 4 hinge ) < 400 > 1 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 1 5 10 < 210 > 2 < 211 > 36 < 212 > DNA < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > spacer ( IgG 4 hinge ) < 400 > 2 gaatctaagt acggaccgcc ctgcccccct tgccct 36 < 210 > 3 < 211 > 119 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > Hinge - CH 3 spacer < 400 > 3 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 1 5 10 15 Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 20 25 30 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 35 40 45 Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 50 55 60 Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 65 70 75 80 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 85 90 95 Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 100 105 110 Leu Ser Leu Ser Leu Gly Lys 115 < 210 > 4 < 211 > 229 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > Hinge - CH 2 - CH 3 spacer < 400 > 4 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 1 5 10 15 Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 20 25 30 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 35 40 45 Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 50 55 60 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 65 70 75 80 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 85 90 95 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 100 105 110 Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 115 120 125 Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 130 135 140 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 145 150 155 160 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 165 170 175 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 180 185 190 Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 195 200 205 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 210 215 220 Leu Ser Leu Gly Lys 225 < 210 > 5 < 211 > 282 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > IgD - hinge - Fc < 400 > 5 Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala 1 5 10 15 Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala 20 25 30 Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys 35 40 45 Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro 50 55 60 Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln 65 70 75 80 Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly 85 90 95 Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val 100 105 110 Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly 115 120 125 Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn 130 135 140 Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro 145 150 155 160 Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys 165 170 175 Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser 180 185 190 Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu 195 200 205 Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro 210 215 220 Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser 225 230 235 240 Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr 245 250 255 Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg 260 265 270 Ser Leu Glu Val Ser Tyr Val Thr Asp His 275 280 < 210 > 6 < 211 > 24 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > T 2 A < 400 > 6 Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 1 5 10 15 Val Glu Glu Asn Pro Gly Pro Arg 20 < 210 > 7 < 211 > 335 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > tEGFR < 400 > 7 Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 1 5 10 15 Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 20 25 30 Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 35 40 45 Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 50 55 60 Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 65 70 75 80 Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 85 90 95 Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 100 105 110 Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 115 120 125 Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 130 135 140 Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 145 150 155 160 Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 165 170 175 Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 180 185 190 Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 195 200 205 Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 210 215 220 Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 225 230 235 240 Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 245 250 255 His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 260 265 270 Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 275 280 285 Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 290 295 300 Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 305 310 315 320 Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 325 330 335 < 210 > 8 < 211 > 27 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 28 < 400 > 8 Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 1 5 10 15 Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 20 25 < 210 > 9 < 211 > 66 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 28 < 400 > 9 Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 1 5 10 15 Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 20 25 30 Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly 35 40 45 Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 50 55 60 Trp Val 65 < 210 > 10 < 211 > 41 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 28 < 400 > 10 Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 1 5 10 15 Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 20 25 30 Pro Arg Asp Phe Ala Ala Tyr Arg Ser 35 40 < 210 > 11 < 211 > 41 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 28 < 400 > 11 Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 1 5 10 15 Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 20 25 30 Pro Arg Asp Phe Ala Ala Tyr Arg Ser 35 40 < 210 > 12 < 211 > 42 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > 4 - 1 BB < 400 > 12 Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 1 5 10 15 Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 20 25 30 Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 35 40 < 210 > 13 < 211 > 112 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 3 zeta < 400 > 13 Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 1 5 10 15 Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 20 25 30 Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 35 40 45 Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 50 55 60 Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 65 70 75 80 Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 85 90 95 Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 100 105 110 < 210 > 14 < 211 > 112 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 3 zeta < 400 > 14 Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln Gly 1 5 10 15 Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 20 25 30 Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 35 40 45 Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 50 55 60 Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 65 70 75 80 Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 85 90 95 Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 100 105 110 < 210 > 15 < 211 > 112 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 3 zeta < 400 > 15 Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 1 5 10 15 Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 20 25 30 Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 35 40 45 Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 50 55 60 Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 65 70 75 80 Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 85 90 95 Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 100 105 110 < 210 > 16 < 211 > 30 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Linker sequence < 400 > 16 Pro Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Pro 20 25 30 < 210 > 17 < 211 > 17 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker sequence < 400 > 17 Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Gly Lys 1 5 10 15 Ser < 210 > 18 < 211 > 18 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > T 2 A < 400 > 18 Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 1 5 10 15 Gly Pro < 210 > 19 < 211 > 22 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > P 2 A < 400 > 19 Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 1 5 10 15 Glu Glu Asn Pro Gly Pro 20 < 210 > 20 < 211 > 19 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > P 2 A < 400 > 20 Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 1 5 10 15 Pro Gly Pro < 210 > 21 < 211 > 20 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > E 2 A < 400 > 21 Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 1 5 10 15 Asn Pro Gly Pro 20 < 210 > 22 < 211 > 22 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > F 2 A < 400 > 22 Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 1 5 10 15 Glu Ser Asn Pro Gly Pro 20 < 210 > 23 < 211 > 357 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > tEGFR < 400 > 23 Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 1 5 10 15 Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly 20 25 30 Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe 35 40 45 Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala 50 55 60 Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu 65 70 75 80 Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile 85 90 95 Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu 100 105 110 Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala 115 120 125 Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu 130 135 140 Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr 145 150 155 160 Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys 165 170 175 Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly 180 185 190 Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu 195 200 205 Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys 210 215 220 Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu 225 230 235 240 Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met 245 250 255 Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala 260 265 270 His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val 275 280 285 Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His 290 295 300 Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro 305 310 315 320 Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala 325 330 335 Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly 340 345 350 Ile Gly Leu Phe Met 355 < 210 > 24 < 211 > 66 < 212 > DNA < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > GMCSFR alpha chain signal sequence < 400 > 24 atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60 atccca 66 < 210 > 25 < 211 > 22 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > GMCSFR alpha chain signal sequence < 400 > 25 Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 1 5 10 15 Ala Phe Leu Leu Ile Pro 20 < 210 > 26 < 211 > 18 < 212 > PRT < 213 > Homo sapiens < 220 > < 221 > misc _ feature < 223 > CD 8 alpha signal peptide < 400 > 26 Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 1 5 10 15 His Ala < 210 > 27 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 27 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15 < 210 > 28 < 211 > 12 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 28 Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 1 5 10 < 210 > 29 < 211 > 61 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 29 Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro 1 5 10 15 Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu 20 25 30 Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu Pro 35 40 45 Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 50 55 60 < 210 > 30 < 211 > 12 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge Sequence < 400 > 30 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 1 5 10 < 210 > 31 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 220 > < 221 > misc _ feature < 222 > ( 1 ) . .( 1 ) < 223 > glycine , cysteine , or arginine < 220 > < 221 > misc _ feature < 222 > ( 4 ) . .( 4 ) < 223 > Xaa is cysteine or threonine < 400 > 31 Xaa Pro Pro Xaa Pro 1 5 < 210 > 32 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 32 Tyr Gly Pro Pro Cys Pro Pro Cys Pro 1 5 < 210 > 33 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 33 Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 1 5 10 < 210 > 34 < 211 > 14 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Hinge sequence < 400 > 34 Glu Val Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 1 5 10 < 210 > 35 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR L 1 < 400 > 35 Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn 1 5 10 < 210 > 36 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR L 2 < 400 > 36 Ser Arg Leu His Ser Gly Val 1 5 < 210 > 37 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR L 3 < 400 > 37 Gly Asn Thr Leu Pro Tyr Thr Phe Gly 1 5 < 210 > 38 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR H 1 < 400 > 38 Asp Tyr Gly Val Ser 1 5 < 210 > 39 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR H 2 < 400 > 39 Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 1 5 10 15 < 210 > 40 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 CDR H 3 < 400 > 40 Tyr Ala Met Asp Tyr Trp Gly 1 5 < 210 > 41 < 211 > 120 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 VH < 400 > 41 Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 1 5 10 15 Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 20 25 30 Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 35 40 45 Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 50 55 60 Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 65 70 75 80 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 85 90 95 Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 100 105 110 Gly Thr Ser Val Thr Val Ser Ser 115 120 < 210 > 42 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 VL < 400 > 42 Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45 Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 65 70 75 80 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 100 105 < 210 > 43 < 211 > 245 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 scFv < 400 > 43 Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45 Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 65 70 75 80 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Ser Thr Ser Gly 100 105 110 Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Val Lys 115 120 125 Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 130 135 140 Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser 145 150 155 160 Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 165 170 175 Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu 180 185 190 Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 195 200 205 Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr 210 215 220 Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 225 230 235 240 Val Thr Val Ser Ser 245 < 210 > 44 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR L 1 < 400 > 44 Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala 1 5 10 < 210 > 45 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR L 2 < 400 > 45 Ser Ala Thr Tyr Arg Asn Ser 1 5 < 210 > 46 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR L 3 < 400 > 46 Gln Gln Tyr Asn Arg Tyr Pro Tyr Thr 1 5 < 210 > 47 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR H 1 < 400 > 47 Ser Tyr Trp Met Asn 1 5 < 210 > 48 < 211 > 17 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR H 2 < 400 > 48 Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 1 5 10 15 Gly < 210 > 49 < 211 > 13 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 CDR H 3 < 400 > 49 Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr 1 5 10 < 210 > 50 < 211 > 122 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 VH < 400 > 50 Glu Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 20 25 30 Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 50 55 60 Lys Gly Gln Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80 Met Gln Leu Ser Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 85 90 95 Ala Arg Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr Trp 100 105 110 Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 51 < 211 > 108 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 VL < 400 > 51 Asp Ile Glu Leu Thr Gln Ser Pro Lys Phe Met Ser Thr Ser Val Gly 1 5 10 15 Asp Arg Val Ser Val Thr Cys Lys Ala Ser Gln Asn Val Gly Thr Asn 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Pro Leu Ile 35 40 45 Tyr Ser Ala Thr Tyr Arg Asn Ser Gly Val Pro Asp Arg Phe Thr Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Asn Val Gln Ser 65 70 75 80 Lys Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Arg Tyr Pro Tyr 85 90 95 Thr Ser Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 100 105 < 210 > 52 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Linker sequence < 400 > 52 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 15 < 210 > 53 < 211 > 245 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > SJ 25 C 1 scFv < 400 > 53 Glu Val Lys Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ser 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 20 25 30 Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 50 55 60 Lys Gly Gln Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80 Met Gln Leu Ser Gly Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 85 90 95 Ala Arg Lys Thr Ile Ser Ser Val Val Asp Phe Tyr Phe Asp Tyr Trp 100 105 110 Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 115 120 125 Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Glu Leu Thr Gln Ser 130 135 140 Pro Lys Phe Met Ser Thr Ser Val Gly Asp Arg Val Ser Val Thr Cys 145 150 155 160 Lys Ala Ser Gln Asn Val Gly Thr Asn Val Ala Trp Tyr Gln Gln Lys 165 170 175 Pro Gly Gln Ser Pro Lys Pro Leu Ile Tyr Ser Ala Thr Tyr Arg Asn 180 185 190 Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe 195 200 205 Thr Leu Thr Ile Thr Asn Val Gln Ser Lys Asp Leu Ala Asp Tyr Phe 210 215 220 Cys Gln Gln Tyr Asn Arg Tyr Pro Tyr Thr Ser Gly Gly Gly Thr Lys 225 230 235 240 Leu Glu Ile Lys Arg 245 < 210 > 54 < 211 > 12 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 HC - CDR 3 < 400 > 54 His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr 1 5 10 < 210 > 55 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 LC - CDR 2 < 400 > 55 His Thr Ser Arg Leu His Ser 1 5 < 210 > 56 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FMC 63 LC - CDR 3 < 400 > 56 Gln Gln Gly Asn Thr Leu Pro Tyr Thr 1 5 < 210 > 57 < 211 > 735 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > sequence encoding scFv < 400 > 57 gacatccaga tgacccagac cacctccagc ctgagcgcca gcctgggcga ccgggtgacc 60 atcagctgcc gggccagcca ggacatcagc aagtacctga actggtatca gcagaagccc 120 gacggcaccg tcaagctgct gatctaccac accagccggc tgcacagcgg cgtgcccagc 180 cggtttagcg gcagcggctc cggcaccgac tacagcctga ccatctccaa cctggaacag 240 gaagatatcg ccacctactt ttgccagcag ggcaacacac tgccctacac ctttggcggc 300 ggaacaaagc tggaaatcac cggcagcacc tccggcagcg gcaagcctgg cagcggcgag 360 ggcagcacca agggcgaggt gaagctgcag gaaagcggcc ctggcctggt ggcccccagc 420 cagagcctga gcgtgacctg caccgtgagc ggcgtgagcc tgcccgacta cggcgtgagc 480 tggatccggc agccccccag gaagggcctg gaatggctgg gcgtgatctg gggcagcgag 540 accacctact acaacagcgc cctgaagagc cggctgacca tcatcaagga caacagcaag 600 agccaggtgt tcctgaagat gaacagcctg cagaccgacg acaccgccat ctactactgc 660 gccaagcact actactacgg cggcagctac gccatggact actggggcca gggcaccagc 720 gtgaccgtga gcagc 735 < 210 > 58 < 211 > 18 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Linker sequence < 400 > 58 Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 1 5 10 15 Lys Gly