시약 은 Dulbecco ' s Modified Eagle ' s Medium ( DMEM ; Gibco BRL , U . S . A . ) , fetal bovine serum ( FBS ; Gibco BRL , U . S . A . ) , trypsin - EDTA ( Thermofisher , U . S . A . ) , trypan blue ( Sigma Co . , U . S . A ) , EZ - Cytox ( Daeilab , Korea ) , dulbecco ' s phosphate buffered saline ( Welgene , Korea ) , RIPA buffer ( Thermo Co . , U . S . A . ) , sample loading buffer ( BIO - RAD Co . , Japan ) , 30 % Acrylamide / Bis Solution 29 : 1 ( 3 . 3 % C ) ( BIO - RAD Co . , Japan ) , Skim Milk ( BD Co . , France ) , Pierce BCA Proein Assay Kit ( Thermo Co . , U . S . A . ) , TEMED ( BIO - RAD Co . , Japan ) , phosphorylation - AKT ( p - AKT ) , AKT , phosphorylation - mTOR ( p - mTOR ) , mTOR antibody ( Danvers . , U . S . A ) , Protease inhibitor cocktail , phosphatase inhibitor cocktail ( Sigma Co . , U . S . A ) , Total RNA prep kit ( Intronbio , Korea ) , AccuPower CycleScript RT PreMix ( Bioneer , Korea ) , SYBR Green ( Qiagen , Germany ) 등 을 사용 하 였 다 .