< 110 > BOARD OF REGENTS , THE UNIVERSITY OF TEXAS SYSTEM < 120 > ANTI - OX 40 ANTIBODIES AND METHODS OF USING THE SAME < 130 > IPA 160758 - US - D 1 < 150 > 61 / 380 , 827 < 151 > 2010 - 09 - 08 < 150 > 61 / 375 , 999 < 151 > 2010 - 08 - 23 < 160 > 24 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 5 < 212 > PRT < 213 > Mus sp . < 400 > 1 Asp Tyr Ser Met His 1 5 < 210 > 2 < 211 > 17 < 212 > PRT < 213 > Mus sp . < 400 > 2 Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys 1 5 10 15 Gly < 210 > 3 < 211 > 13 < 212 > PRT < 213 > Mus sp . < 400 > 3 Pro Tyr Tyr Asp Tyr Val Ser Tyr Tyr Ala Met Asp Tyr 1 5 10 < 210 > 4 < 211 > 122 < 212 > PRT < 213 > Mus sp . < 400 > 4 Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 1 5 10 15 Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 20 25 30 Ser Met His Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met 35 40 45 Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 50 55 60 Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 65 70 75 80 Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys 85 90 95 Ala Asn Pro Tyr Tyr Asp Tyr Val Ser Tyr Tyr Ala Met Asp Tyr Trp 100 105 110 Gly His Gly Thr Ser Val Thr Val Ser Ser 115 120 < 210 > 5 < 211 > 122 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polypeptide < 400 > 5 Gln Val Gln Leu Val Gln Ser Gly Ser Glu Leu Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 20 25 30 Ser Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Lys Trp Met 35 40 45 Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe 50 55 60 Lys Gly Arg Phe Val Phe Ser Leu Asp Thr Ser Val Ser Thr Ala Tyr 65 70 75 80 Leu Gln Ile Ser Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Asn Pro Tyr Tyr Asp Tyr Val Ser Tyr Tyr Ala Met Asp Tyr Trp 100 105 110 Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 6 < 211 > 458 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polynucleotide < 400 > 6 actagtacca ccatggcttg ggtgtggacc ttgctattcc tgatggcagc tgcccaaagt 60 atccaagcac aggttcagtt ggtgcagtct ggatctgagc tgaagaagcc tggagcctca 120 gtcaaggttt cctgcaaggc ttctggttat accttcacag actattcaat gcactgggtg 180 cgacaggctc caggacaagg tttaaagtgg atgggctgga taaacactga gactggtgag 240 ccaacatatg cagatgactt caagggacgg tttgtcttct ctttggacac ctctgtcagc 300 actgcctatt tgcagatcag cagcctcaaa gctgaggaca cggctgtgta ttactgtgct 360 aatccctact atgattacgt ctcttactat gctatggact actggggtca gggaaccacg 420 gtcaccgtct cctcaggtaa gaatggcctc tcaagctt 458 < 210 > 7 < 211 > 11 < 212 > PRT < 213 > Mus sp . < 400 > 7 Lys Ala Ser Gln Asp Val Ser Thr Ala Val Ala 1 5 10 < 210 > 8 < 211 > 7 < 212 > PRT < 213 > Mus sp . < 400 > 8 Ser Ala Ser Tyr Leu Tyr Thr 1 5 < 210 > 9 < 211 > 9 < 212 > PRT < 213 > Mus sp . < 400 > 9 Gln Gln His Tyr Ser Thr Pro Arg Thr 1 5 < 210 > 10 < 211 > 107 < 212 > PRT < 213 > Mus sp . < 400 > 10 Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Arg 1 5 10 15 Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ala Ser Tyr Leu Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 65 70 75 80 Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Arg 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100 105 < 210 > 11 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polypeptide < 400 > 11 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Thr Ala 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ala Ser Tyr Leu Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln His Tyr Ser Thr Pro Arg 85 90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105 < 210 > 12 < 211 > 416 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polynucleotide < 400 > 12 gctagcacca ccatggagtc acagattcag gtctttgtat tcgtgtttct ctggttgtct 60 ggtgttgacg gagacattca gatgacccag tctccatcct ccctgtccgc atcagtggga 120 gacagggtca ccatcacctg caaggccagt caggatgtga gtactgctgt agcctggtat 180 caacagaaac caggaaaagc ccctaaacta ctgatttact cggcatccta cctctacact 240 ggagtccctt cacgcttcag tggcagtgga tctgggacgg atttcacttt caccatcagc 300 agtctgcagc ctgaagacat tgcaacatat tactgtcagc aacattatag tactcctcgg 360 acgttcggtc agggcaccaa gctggaaatc aaacgtaagt agaatccaaa gaattc 416 < 210 > 13 < 211 > 5 < 212 > PRT < 213 > Mus sp . < 400 > 13 Ser His Asp Met Ser 1 5 < 210 > 14 < 211 > 17 < 212 > PRT < 213 > Mus sp . < 400 > 14 Ala Ile Asn Ser Asp Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Met Glu 1 5 10 15 Arg < 210 > 15 < 211 > 11 < 212 > PRT < 213 > Mus sp . < 400 > 15 His Tyr Asp Asp Tyr Tyr Ala Trp Phe Ala Tyr 1 5 10 < 210 > 16 < 211 > 120 < 212 > PRT < 213 > Mus sp . < 400 > 16 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Glu 1 5 10 15 Ser Leu Lys Leu Ser Cys Glu Ser Asn Glu Tyr Glu Phe Pro Ser His 20 25 30 Asp Met Ser Trp Val Arg Lys Thr Pro Glu Lys Arg Leu Glu Leu Val 35 40 45 Ala Ala Ile Asn Ser Asp Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Met 50 55 60 Glu Arg Arg Phe Ile Ile Ser Arg Asp Asn Thr Lys Lys Thr Leu Tyr 65 70 75 80 Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Leu Tyr Tyr Cys 85 90 95 Ala Arg His Tyr Asp Asp Tyr Tyr Ala Trp Phe Ala Tyr Trp Gly Gln 100 105 110 Gly Thr Leu Val Thr Val Ser Ala 115 120 < 210 > 17 < 211 > 120 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polypeptide < 400 > 17 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Tyr Glu Phe Pro Ser His 20 25 30 Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Leu Val 35 40 45 Ala Ala Ile Asn Ser Asp Gly Gly Ser Thr Tyr Tyr Pro Asp Thr Met 50 55 60 Glu Arg Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg His Tyr Asp Asp Tyr Tyr Ala Trp Phe Ala Tyr Trp Gly Gln 100 105 110 Gly Thr Met Val Thr Val Ser Ser 115 120 < 210 > 18 < 211 > 451 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polynucleotide < 400 > 18 actagtacca ccatggactt cgggctcagc ttggttttcc ttgtccttat tttaaaaagt 60 gtacagtgtg aggtgcagct ggtggagtct gggggaggct tagtgcagcc tggagggtcc 120 ctgagactct cctgtgcagc ctctgaatac gagttccctt cccatgacat gtcttgggtc 180 cgccaggctc cggggaaggg gctggagttg gtcgcagcca ttaatagtga tggtggtagc 240 acctactatc cagacaccat ggagagacga ttcaccatct ccagagacaa tgccaagaac 300 tcactgtacc tgcaaatgaa cagtctgagg gccgaggaca cagccgtgta ttactgtgca 360 agacactatg atgattacta cgcctggttt gcttactggg gccaagggac tatggtcact 420 gtctcttcag gtgagtccta acttcaagct t 451 < 210 > 19 < 211 > 15 < 212 > PRT < 213 > Mus sp . < 400 > 19 Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr Ser Tyr Met His 1 5 10 15 < 210 > 20 < 211 > 7 < 212 > PRT < 213 > Mus sp . < 400 > 20 Leu Ala Ser Asn Leu Glu Ser 1 5 < 210 > 21 < 211 > 9 < 212 > PRT < 213 > Mus sp . < 400 > 21 Gln His Ser Arg Glu Leu Pro Leu Thr 1 5 < 210 > 22 < 211 > 111 < 212 > PRT < 213 > Mus sp . < 400 > 22 Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Lys Ser Val Ser Thr Ser 20 25 30 Gly Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 35 40 45 Lys Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 50 55 60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 65 70 75 80 Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ser Arg 85 90 95 Glu Leu Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 100 105 110 < 210 > 23 < 211 > 111 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polypeptide < 400 > 23 Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Lys Ser Val Ser Thr Ser 20 25 30 Gly Tyr Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 35 40 45 Arg Leu Leu Ile Tyr Leu Ala Ser Asn Leu Glu Ser Gly Val Pro Ala 50 55 60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 65 70 75 80 Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln His Ser Arg 85 90 95 Glu Leu Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 110 < 210 > 24 < 211 > 428 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Description of Artificial Sequence : Synthetic polynucleotide < 400 > 24 gctagcacca ccatggagac agacacactc ctgttatggg tactgctgct ctgggttcca 60 ggttccactg gtgaaattgt gctgacacag tctcctgcta ccttatcttt gtctccaggg 120 gaaagggcca ccctctcatg cagggccagc aaaagtgtca gtacatctgg ctatagttat 180 atgcactggt accaacagaa accaggacag gctcccagac tcctcatcta tcttgcatcc 240 aacctagaat ctggggtccc tgccaggttc agtggcagtg ggtctgggac agacttcacc 300 ctcaccatca gcagcctaga gcctgaggat tttgcagttt attactgtca gcacagtagg 360 gagcttccgc tcacgttcgg cggagggacc aaggtcgaga tcaaacgtaa gtacactttt 420 ctgaattc 428