< 110 > Roche Glycart AG < 120 > Anti - Tenascin - C A 2 Antibodies and Methods of Use < 130 > 26952 < 140 > PCT / EP 2011 / 063734 < 141 > 2011 - 08 - 10 < 150 > EP 10172841 . 8 < 151 > 2010 - 08 - 13 < 160 > 136 < 170 > PatentIn version 3 . 5 < 210 > 1 < 211 > 1611 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Library Template for DP 47 - 3 library < 400 > 1 atgaaatacc tattgcctac ggcagccgct ggattgttat tactcgcggc ccagccggcc 60 atggccgaaa tcgtgttaac gcagtctcca ggcaccctgt ctttgtctcc aggggaaaga 120 gccaccctct cttgcagggc cagtcagagt gttagcagca gctacttagc ctggtaccag 180 cagaaacctg gccaggctcc caggctcctc atctatggag catccagcag ggccactggc 240 atcccagaca ggttcagtgg cagtggatcc gggacagact tcactctcac catcagcaga 300 ctggagcctg aagattttgc agtgtattac tgtcagcagt atggtagctc accgctgacg 360 ttcggccagg ggaccaaagt ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc 420 ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat 480 aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt 540 aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc 600 accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc 660 catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg tggagccgca 720 gaacaaaaac tcatctcaga agaggatctg aatggagccg cagactacaa ggacgacgac 780 gacaagggtg ccgcataata aggcgcgcca attctatttc aaggagacag tcatatgaaa 840 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc 900 gaggtgcaat tgctggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc 960 tcctgtgcag cctccggatt cacctttagc agttatgcca tgagctgggt ccgccaggct 1020 ccagggaagg ggctggagtg ggtctcagct attagtggta gtggtggtag cacatactac 1080 gcagactccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat 1140 ctgcagatga acagcctgag agccgaggac acggccgtat attactgtgc gaaaccgttt 1200 ccgtattttg actactgggg ccaaggaacc ctggtcaccg tctcgagtgc tagcaccaaa 1260 ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc 1320 ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc 1380 gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc 1440 ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac 1500 gtgaatcaca agcccagcaa caccaaagtg gacaagaaag ttgagcccaa atcttgtgac 1560 gcggccgcaa gcactagtgc ccatcaccat caccatcacg ccgcggcata g 1611 < 210 > 2 < 211 > 1617 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Library Template for DP 88 - 3 library < 400 > 2 atgaaatacc tattgcctac ggcagccgct ggattgttat tactcgcggc ccagccggcc 60 atggccgata tccagatgac ccagtctcca tcctccctgt ctgcatctgt cggagaccgg 120 gtcaccatca cctgccgggc aagtcagggc attagaaatg atttaggctg gtaccagcag 180 aagccaggga aagcccctaa gcgcctgatc tatgctgcat ccagtttgca gagtggcgtc 240 ccatcaaggt tcagcggcag tggatccggg acagagttca ctctcaccat cagcagcttg 300 cagcctgaag attttgccac ctattactgc ttgcagcata atagttaccc cacgtttggc 360 cagggcacca aagtcgagat caagcgtacg gtggctgcac catctgtctt catcttcccg 420 ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc 480 tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc 540 caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg 600 acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag 660 ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgtggagc cgcagaacaa 720 aaactcatct cagaagagga tctgaatgga gccgcagact acaaggacga cgacgacaag 780 ggtgccgcat aataaggcgc gccaattcta tttcaaggag acagtcatat gaaatacctg 840 ctgccgaccg ctgctgctgg tctgctgctc ctcgctgccc agccggcgat ggcccaggtg 900 caattggtgc agtctggggc tgaggtgaag aagcctgggt cctcggtgaa ggtctcctgc 960 aaggcctccg gaggcacatt cagcagctac gctataagct gggtgcgaca ggcccctgga 1020 caagggctcg agtggatggg agggatcatc cctatctttg gtacagcaaa ctacgcacag 1080 aagttccagg gcagggtcac cattactgca gacaaatcca cgagcacagc ctacatggag 1140 ctgagcagcc tgagatctga ggacaccgcc gtgtattact gtgcgagact atccccaggc 1200 ggttactatg ttatggatgc ctggggccaa gggaccaccg tgaccgtctc ctcagctagc 1260 accaaaggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca 1320 gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac 1380 tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc 1440 tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc 1500 tgcaacgtga atcacaagcc cagcaacacc aaagtggaca agaaagttga gcccaaatct 1560 tgtgacgcgg ccgcaagcac tagtgcccat caccatcacc atcacgccgc ggcatag 1617 < 210 > 3 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Kabat < 400 > 3 Ser Tyr Ala Ile Ser 1 5 < 210 > 4 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Kabat ( DNA 1 ) < 400 > 4 agctacgcta taagc 15 < 210 > 5 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Chothia < 400 > 5 Gly Gly Thr Phe Ser Ser Tyr 1 5 < 210 > 6 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Chothia ( DNA 1 ) < 400 > 6 ggaggcacat tcagcagcta c 21 < 210 > 7 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 AbM < 400 > 7 Gly Gly Thr Phe Ser Ser Tyr Ala Ile Ser 1 5 10 < 210 > 8 < 211 > 30 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 AbM ( DNA 1 ) < 400 > 8 ggaggcacat tcagcagcta cgctataagc 30 < 210 > 9 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 1 ) < 400 > 9 Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe Gln 1 5 10 15 < 210 > 10 < 211 > 48 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 1 ) ( DNA ) < 400 > 10 gggatcatcc ctatctttgg tacagcaaac tacgcacaga agttccag 48 < 210 > 11 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 2 ) < 400 > 11 Ala Ile Ile Pro Ile Leu Gly Ile Ala Asn Tyr Ala Gln Lys Phe Gln 1 5 10 15 < 210 > 12 < 211 > 48 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 2 ) ( DNA ) < 400 > 12 gctatcatcc cgatccttgg tatcgcaaac tacgcacaga agttccag 48 < 210 > 13 < 211 > 16 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 3 ) < 400 > 13 Val Ile Ile Pro Ile Leu Gly Thr Ala Asn Tyr Ala Gln Lys Phe Gln 1 5 10 15 < 210 > 14 < 211 > 48 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Kabat ( 3 ) ( DNA ) < 400 > 14 gtgatcatcc ctatccttgg taccgcaaac tacgcacaga agttccag 48 < 210 > 15 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 1 ) < 400 > 15 Ile Pro Ile Phe Gly Thr Ala Asn 1 5 < 210 > 16 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 1 ) ( DNA ) < 400 > 16 atccctatct ttggtacagc a 21 < 210 > 17 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 2 ) < 400 > 17 Ile Pro Ile Leu Gly Ile Ala Asn 1 5 < 210 > 18 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 2 ) ( DNA ) < 400 > 18 atcccgatcc ttggtatcgc a 21 < 210 > 19 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 3 ) < 400 > 19 Ile Pro Ile Leu Gly Thr Ala Asn 1 5 < 210 > 20 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 Chothia ( 3 ) ( DNA ) < 400 > 20 atccctatcc ttggtaccgc a 21 < 210 > 21 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 1 ) < 400 > 21 Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn 1 5 10 < 210 > 22 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 1 ) ( DNA ) < 400 > 22 gggatcatcc ctatctttgg tacagca 27 < 210 > 23 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 2 ) < 400 > 23 Ala Ile Ile Pro Ile Leu Gly Ile Ala Asn 1 5 10 < 210 > 24 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 2 ) ( DNA ) < 400 > 24 gctatcatcc cgatccttgg tatcgca 27 < 210 > 25 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 3 ) < 400 > 25 Val Ile Ile Pro Ile Leu Gly Thr Ala Asn 1 5 10 < 210 > 26 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 2 AbM ( 3 ) ( DNA ) < 400 > 26 gtgatcatcc ctatccttgg taccgca 27 < 210 > 27 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HCDR 3 < 400 > 27 Tyr Cys Ala Arg Leu Tyr Gly Tyr 1 5 < 210 > 28 < 211 > 24 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 3 ( DNA ) < 400 > 28 tactgtgcga gactgtacgg ttac 24 < 210 > 29 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 1 ) < 400 > 29 Arg Ala Ser Gln Gly Ile Arg Asn Asp Leu Gly 1 5 10 < 210 > 30 < 211 > 33 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 1 ) ( DNA 1 ) < 400 > 30 cgggcaagtc agggcattag aaatgattta ggc 33 < 210 > 31 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 2 ) < 400 > 31 Arg Ala Ser Gln Gly Ile Arg Asn Val Leu Gly 1 5 10 < 210 > 32 < 211 > 33 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 2 ) ( DNA 1 ) < 400 > 32 cgggcaagtc agggcattcg taatgtttta ggc 33 < 210 > 33 < 211 > 11 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 3 ) < 400 > 33 Arg Ala Ser Gln Ser Ile Arg Asn Val Leu Gly 1 5 10 < 210 > 34 < 211 > 33 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 3 ) ( DNA ) < 400 > 34 cgggcaagtc agagcattcg taatgtttta ggc 33 < 210 > 35 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 1 ) < 400 > 35 Ala Ala Ser Ser Leu Gln Ser 1 5 < 210 > 36 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 1 ) ( DNA ) < 400 > 36 gctgcatcca gtttgcagag t 21 < 210 > 37 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 2 ) < 400 > 37 Asp Ser Ser Ser Leu Gln Ser 1 5 < 210 > 38 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 2 ) ( DNA ) < 400 > 38 gattcgtcca gtttgcagag t 21 < 210 > 39 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 3 ) < 400 > 39 Asp Ala Tyr Ser Leu Gln Ser 1 5 < 210 > 40 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 3 ) ( DNA ) < 400 > 40 gatgcttaca gcttgcagag t 21 < 210 > 41 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 4 ) < 400 > 41 Asp Val Ser Ser Leu Gln Ser 1 5 < 210 > 42 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 4 ) ( DNA ) < 400 > 42 gatgtgtcca gtttgcagag t 21 < 210 > 43 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 5 ) < 400 > 43 Asp Ala Ser Ser Leu Gln Ser 1 5 < 210 > 44 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 5 ) ( DNA ) < 400 > 44 gatgcgtcca gtttgcagag t 21 < 210 > 45 < 211 > 7 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 6 ) < 400 > 45 Ala Ala Thr Ser Leu Gln Ser 1 5 < 210 > 46 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 2 ( 6 ) ( DNA ) < 400 > 46 gctgctacca gtttgcagag t 21 < 210 > 47 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > LCDR 3 < 400 > 47 Leu Gln Asn Gly Leu Gln Pro Ala Thr 1 5 < 210 > 48 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 3 ( DNA 1 ) < 400 > 48 ttgcagaatg gtctgcagcc cgcgacg 27 < 210 > 49 < 211 > 15 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Kabat ( DNA 2 ) < 400 > 49 agctatgcta taagc 15 < 210 > 50 < 211 > 21 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 Chothia ( DNA 2 ) < 400 > 50 ggaggcacat tcagcagcta t 21 < 210 > 51 < 211 > 30 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HCDR 1 AbM ( DNA 2 ) < 400 > 51 ggaggcacat tcagcagcta tgctataagc 30 < 210 > 52 < 211 > 33 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 1 ) ( DNA 2 ) < 400 > 52 cgggcaagtc aggggattcg taatgattta ggc 33 < 210 > 53 < 211 > 33 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 1 ( 2 ) ( DNA 2 ) < 400 > 53 cgggcaagtc aggggattcg taatgtttta ggc 33 < 210 > 54 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LCDR 3 ( DNA 2 ) < 400 > 54 ctgcagaatg gtctgcagcc cgcgacg 27 < 210 > 55 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ; VL < 400 > 55 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 56 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ; VL < 400 > 56 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattaga aatgatttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgct gcatccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 57 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ( GS ) ; VL < 400 > 57 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 58 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ( GS ) ; VL < 400 > 58 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattaga aatgatttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgct gcatccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcagtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 59 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ; VH < 400 > 59 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 60 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 ; VH < 400 > 60 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 61 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 B 6 ; VL < 400 > 61 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 62 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 B 6 ; VL < 400 > 62 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattaga aatgatttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgct gcatccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcagtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 63 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 B 6 ; VH < 400 > 63 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Ala Ile Ile Pro Ile Leu Gly Ile Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 64 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 B 6 ; VH < 400 > 64 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggagct atcatcccga tccttggtat cgcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 65 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 A 12 ; VL < 400 > 65 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 66 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 A 12 ; VL < 400 > 66 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattaga aatgatttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgct gcatccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcagtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 67 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 A 12 ; VH < 400 > 67 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Val Ile Ile Pro Ile Leu Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 68 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 A 12 ; VH < 400 > 68 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctatgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggagtg atcatcccta tccttggtac cgcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 69 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 A 6 ; VL < 400 > 69 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Val 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Asp Ser Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 70 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 A 6 ; VL < 400 > 70 gacatccaga tgacccagtc tccttcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattcgt aatgttttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgat tcgtccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 71 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 A 6 ; VH < 400 > 71 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 72 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ C 3 A 6 ; VH < 400 > 72 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 73 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ wt ; VL < 400 > 73 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Val 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Asp Ala Tyr Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 74 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ wt ; VL < 400 > 74 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca ggggattcgt aatgttttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgat gcttacagct tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 75 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ wt ; VH < 400 > 75 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 76 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ wt ; VH < 400 > 76 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 77 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ VD ; VL < 400 > 77 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Asp 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Asp Ala Tyr Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 78 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ VD ; VL < 400 > 78 gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca ggggattcgt aatgatttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgat gcttacagct tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 79 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ VD ; VH < 400 > 79 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 80 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ D 1 A 2 _ VD ; VH < 400 > 80 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 81 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 7 D 8 ; VL < 400 > 81 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Arg Asn Val 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Asp Val Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 82 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 7 D 8 ; VL < 400 > 82 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gagcattcgt aatgttttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgat gtgtccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 83 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 7 D 8 ; VH < 400 > 83 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 84 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 7 D 8 ; VH < 400 > 84 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 85 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 1 F 7 ; VL < 400 > 85 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Val 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Tyr Asp Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 86 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 1 F 7 ; VL < 400 > 86 gatatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattcgt aatgttttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatctatgat gcgtccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcctgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 87 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 1 F 7 ; VH < 400 > 87 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 88 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ O 1 F 7 ; VH < 400 > 88 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 89 < 211 > 107 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 H 10 ; VL < 400 > 89 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Arg Asn Val 20 25 30 Leu Gly Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile 35 40 45 Gln Ala Ala Thr Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Gly Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Asn Gly Leu Gln Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 < 210 > 90 < 211 > 321 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 H 10 ; VL < 400 > 90 gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtcggaga ccgggtcacc 60 atcacctgcc gggcaagtca gggcattcgt aatgttttag gctggtacca gcagaagcca 120 gggaaagccc ctaagcgcct gatccaggct gctaccagtt tgcagagtgg cgtcccatca 180 aggttcagcg gcggtggatc cgggacagag ttcactctca ccatcagcag cttgcagcct 240 gaagattttg ccacctatta ctgcttgcag aatggtctgc agcccgcgac gtttggccag 300 ggcaccaaag tcgagatcaa g 321 < 210 > 91 < 211 > 121 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 H 10 ; VH < 400 > 91 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 20 25 30 Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Leu Tyr Gly Tyr Ala Tyr Tyr Gly Ala Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 < 210 > 92 < 211 > 363 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > 2 B 10 _ 6 H 10 ; VH < 400 > 92 caggtgcaat tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc 60 tcctgcaagg cctccggagg cacattcagc agctacgcta taagctgggt gcgacaggcc 120 cctggacaag ggctcgagtg gatgggaggg atcatcccta tctttggtac agcaaactac 180 gcacagaagt tccagggcag ggtcaccatt actgcagaca aatccacgag cacagcctac 240 atggagctga gcagcctgag atctgaggac accgccgtgt attactgtgc gagactgtac 300 ggttacgctt actacggtgc ttttgactac tggggccaag ggaccaccgt gaccgtctcc 360 tca 363 < 210 > 93 < 211 > 330 < 212 > PRT < 213 > Homo sapiens < 400 > 93 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 225 230 235 240 Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 325 330 < 210 > 94 < 211 > 990 < 212 > DNA < 213 > Homo sapiens < 400 > 94 gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg 60 ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg 120 tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca 180 ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc 240 tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc 300 aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga 360 ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct 420 gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg 480 tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac 540 agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag 600 gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc 660 aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag 720 ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc 780 gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg 840 ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg 900 cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg 960 cagaagagcc tctccctgtc tccgggtaaa 990 < 210 > 95 < 211 > 107 < 212 > PRT < 213 > Homo sapiens < 400 > 95 Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 1 5 10 15 Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 20 25 30 Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 35 40 45 Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 50 55 60 Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 65 70 75 80 Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 85 90 95 Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 100 105 < 210 > 96 < 211 > 321 < 212 > DNA < 213 > Homo sapiens < 400 > 96 cgtacggtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct 60 ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag 120 tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac 180 agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag 240 aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag 300 agcttcaaca ggggagagtg t 321 < 210 > 97 < 211 > 119 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Human TNC - A 2 avi - tag his 6 - tag < 400 > 97 Ala Ser Thr Gly Glu Thr Pro Asn Leu Gly Glu Val Val Val Ala Glu 1 5 10 15 Val Gly Trp Asp Ala Leu Lys Leu Asn Trp Thr Ala Pro Glu Gly Ala 20 25 30 Tyr Glu Tyr Phe Phe Ile Gln Val Gln Glu Ala Asp Thr Val Glu Ala 35 40 45 Ala Gln Asn Leu Thr Val Pro Gly Gly Leu Arg Ser Thr Asp Leu Pro 50 55 60 Gly Leu Lys Ala Ala Thr His Tyr Thr Ile Thr Ile Arg Gly Val Thr 65 70 75 80 Gln Asp Phe Ser Thr Thr Pro Leu Ser Val Glu Val Leu Thr Ala Ser 85 90 95 Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Gly 100 105 110 Thr His His His His His His 115 < 210 > 98 < 211 > 357 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Human TNC - A 2 avi - tag his 6 - tag < 400 > 98 gcgtccaccg gggaaacccc gaacctgggc gaagtggtgg tggcggaagt gggttgggat 60 gcgctgaaac tgaactggac cgcgccggaa ggcgcgtatg aatatttttt catccaggtg 120 caggaagcgg ataccgttga agcggcgcag aacctgaccg ttccgggcgg tctgcgtagc 180 accgatctgc cgggcctgaa agcggcgacc cattatacca ttaccatccg tggggtgacc 240 caggatttta gcaccacccc gctgtctgtg gaagtgctga ccgctagcgg cctgaacgac 300 atcttcgagg ctcagaaaat cgaatggcac gaaggtaccc atcaccatca ccaccac 357 < 210 > 99 < 211 > 670 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 huA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7477 < 400 > 99 Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 1 5 10 15 Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 20 25 30 Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 35 40 45 Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 50 55 60 Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 65 70 75 80 Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 85 90 95 Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 100 105 110 Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 115 120 125 Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 130 135 140 Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 145 150 155 160 Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 165 170 175 Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 180 185 190 Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 195 200 205 Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser 210 215 220 Gly Ser Gly His His His His His His Ser Ala Gly Leu Val Pro Arg 225 230 235 240 Gly Ser Thr Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu 245 250 255 Arg Gln His Met Asp Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly 260 265 270 Ile Glu Gly Arg Gly Ser Met Gly Tyr Arg Gly Ser Glu Leu Asp Thr 275 280 285 Pro Lys Asp Leu Gln Val Ser Glu Thr Ala Glu Thr Ser Leu Thr Leu 290 295 300 Leu Trp Lys Thr Pro Leu Ala Lys Phe Asp Arg Tyr Arg Leu Asn Tyr 305 310 315 320 Ser Leu Pro Thr Gly Gln Trp Val Gly Val Gln Leu Pro Arg Asn Thr 325 330 335 Thr Ser Tyr Val Leu Arg Gly Leu Glu Pro Gly Gln Glu Tyr Asn Val 340 345 350 Leu Leu Thr Ala Glu Lys Gly Arg His Lys Ser Lys Pro Ala Arg Val 355 360 365 Lys Ala Ser Thr Glu Gln Ala Pro Glu Leu Glu Asn Leu Thr Val Thr 370 375 380 Glu Val Gly Trp Asp Gly Leu Arg Leu Asn Trp Thr Ala Ala Asp Gln 385 390 395 400 Ala Tyr Glu His Phe Ile Ile Gln Val Gln Glu Ala Asn Lys Val Glu 405 410 415 Ala Ala Arg Asn Leu Thr Val Pro Gly Ser Leu Arg Ala Val Asp Ile 420 425 430 Pro Gly Leu Lys Ala Ala Thr Pro Tyr Thr Val Ser Ile Tyr Gly Val 435 440 445 Ile Gln Gly Tyr Arg Thr Pro Val Leu Ser Ala Glu Ala Ser Thr Gly 450 455 460 Glu Thr Pro Asn Leu Gly Glu Val Val Val Ala Glu Val Gly Trp Asp 465 470 475 480 Ala Leu Lys Leu Asn Trp Thr Ala Pro Glu Gly Ala Tyr Glu Tyr Phe 485 490 495 Phe Ile Gln Val Gln Glu Ala Asp Thr Val Glu Ala Ala Gln Asn Leu 500 505 510 Thr Val Pro Gly Gly Leu Arg Ser Thr Asp Leu Pro Gly Leu Lys Ala 515 520 525 Ala Thr His Tyr Thr Ile Thr Ile Arg Gly Val Thr Gln Asp Phe Ser 530 535 540 Thr Thr Pro Leu Ser Val Glu Val Leu Thr Glu Glu Val Pro Asp Met 545 550 555 560 Gly Asn Leu Thr Val Thr Glu Val Ser Trp Asp Ala Leu Arg Leu Asn 565 570 575 Trp Thr Thr Pro Asp Gly Thr Tyr Asp Gln Phe Thr Ile Gln Val Gln 580 585 590 Glu Ala Asp Gln Val Glu Glu Ala His Asn Leu Thr Val Pro Gly Ser 595 600 605 Leu Arg Ser Met Glu Ile Pro Gly Leu Arg Ala Gly Thr Pro Tyr Thr 610 615 620 Val Thr Leu His Gly Glu Val Arg Gly His Ser Thr Arg Pro Leu Ala 625 630 635 640 Val Glu Val Val Thr Ala Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln 645 650 655 Lys Ile Glu Trp His Glu Gly Thr His His His His His His 660 665 670 < 210 > 100 < 211 > 2013 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 huA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7477 ( DNA ) < 400 > 100 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa 120 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat 180 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac 240 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg 300 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt 360 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa 420 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat 480 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa 540 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca 600 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat 660 ggttcaacta gtggttctgg tcatcaccat caccatcact ccgcgggtct ggtgccacgc 720 ggtagtactg caattggtat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg 780 gacagcccag atctgggtac cggtggtggc tccggtattg agggacgcgg gtccatggga 840 tatcggggat ccgagctgga cacccccaag gacctgcagg tgtccgagac agccgagaca 900 agcctgaccc tgctgtggaa aacccccctg gccaagttcg accggtacag actgaactac 960 agcctgccca ctggacagtg ggtcggcgtg cagctgcccc ggaacaccac ctcctacgtg 1020 ctgcggggcc tggaacccgg ccaggaatac aacgtcctgc tgacggccga gaagggccgg 1080 cacaagagca agcccgccag agtgaaggcc agcaccgagc aggcccccga gctggaaaac 1140 ctgaccgtga ccgaagtggg ctgggacggc ctgcggctga actggaccgc ggctgaccag 1200 gcctatgagc actttatcat tcaggtgcag gaggccaaca aggtggaggc agctcggaac 1260 ctcaccgtgc ctggcagcct tcgggctgtg gacataccgg gcctcaaggc tgctacgcct 1320 tatacagtct ccatctatgg ggtgatccag ggctatagaa caccagtgct ctctgctgag 1380 gcctccacag gcgaaacacc gaacctgggc gaagtggtgg tggcggaagt gggttgggat 1440 gcgctgaaac tgaactggac cgcgccggaa ggcgcgtatg aatatttttt catccaggtg 1500 caggaagcgg ataccgttga agcggcgcag aacctgaccg ttccgggcgg tctgcgtagc 1560 accgatctgc cgggcctgaa agcggcgacc cattatacca ttaccatccg tggggtgacc 1620 caggacttct ctaccacccc tctgagcgtg gaggtgctga ccgaggaggt acccgacatg 1680 ggcaacctga ccgtgaccga ggtgtcctgg gacgccctgc ggctgaactg gaccaccccc 1740 gacggcacct acgaccagtt cacaatccag gtgcaggaag ccgaccaggt cgaagaggcc 1800 cacaatctga cagtgccagg cagcctgcgg agcatggaaa tccccggcct gagagccggc 1860 accccctaca ccgtgaccct gcacggcgaa gtgcggggcc acagcaccag acccctggcc 1920 gtggaagtgg tcacagctag cggcctgaac gacatcttcg aggctcagaa aatcgaatgg 1980 cacgaaggta cccatcacca tcaccaccac taa 2013 < 210 > 101 < 211 > 670 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 cyA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7478 < 400 > 101 Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 1 5 10 15 Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 20 25 30 Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 35 40 45 Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 50 55 60 Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 65 70 75 80 Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 85 90 95 Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 100 105 110 Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 115 120 125 Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 130 135 140 Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 145 150 155 160 Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 165 170 175 Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 180 185 190 Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 195 200 205 Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser 210 215 220 Gly Ser Gly His His His His His His Ser Ala Gly Leu Val Pro Arg 225 230 235 240 Gly Ser Thr Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu 245 250 255 Arg Gln His Met Asp Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly 260 265 270 Ile Glu Gly Arg Gly Ser Met Gly Tyr Arg Gly Ser Glu Leu Asp Thr 275 280 285 Pro Lys Asp Leu Gln Val Ser Glu Thr Ala Glu Thr Ser Leu Thr Leu 290 295 300 Leu Trp Lys Thr Pro Leu Ala Lys Phe Asp Arg Tyr Arg Leu Asn Tyr 305 310 315 320 Ser Leu Pro Thr Gly Gln Trp Val Gly Val Gln Leu Pro Arg Asn Thr 325 330 335 Thr Ser Tyr Val Leu Arg Gly Leu Glu Pro Gly Gln Glu Tyr Asn Val 340 345 350 Leu Leu Thr Ala Glu Lys Gly Arg His Lys Ser Lys Pro Ala Arg Val 355 360 365 Lys Ala Ser Thr Glu Gln Ala Pro Glu Leu Glu Asn Leu Thr Val Thr 370 375 380 Glu Val Gly Trp Asp Gly Leu Arg Leu Asn Trp Thr Ala Ala Asp Gln 385 390 395 400 Ala Tyr Glu His Phe Val Ile Gln Val Gln Glu Ala Asn Lys Val Glu 405 410 415 Ala Ala Gln Asn Leu Thr Val Pro Gly Asn Leu Arg Ala Val Asp Ile 420 425 430 Pro Gly Leu Lys Ala Ala Thr Pro Tyr Thr Val Ser Ile Tyr Gly Val 435 440 445 Ile Gln Gly Tyr Arg Thr Pro Val Leu Ser Ala Glu Ala Ser Thr Gly 450 455 460 Glu Thr Pro Asn Leu Gly Glu Val Met Val Ser Glu Val Gly Trp Asp 465 470 475 480 Ala Leu Lys Leu Asn Trp Thr Val Pro Glu Gly Ala Tyr Glu Tyr Phe 485 490 495 Phe Ile Gln Val Gln Glu Ala Asp Thr Val Glu Ala Ala Gln Asn His 500 505 510 Thr Val Pro Gly Gly Leu Arg Ser Thr Asp Leu Pro Gly Leu Lys Ala 515 520 525 Ala Thr His Tyr Thr Ile Thr Ile Arg Gly Val Thr Gln Asp Phe Ser 530 535 540 Thr Thr Pro Leu Ser Val Glu Val Leu Thr Glu Glu Val Pro Asp Met 545 550 555 560 Gly Asn Leu Thr Val Thr Glu Val Ser Trp Asp Ala Leu Arg Leu Asn 565 570 575 Trp Thr Thr Pro Asp Gly Thr Tyr Asp Gln Phe Thr Ile Gln Val Gln 580 585 590 Glu Ala Asp Gln Val Glu Glu Ala His Asn Leu Thr Val Pro Gly Ser 595 600 605 Leu Arg Ser Met Glu Ile Pro Gly Leu Arg Ala Gly Thr Pro Tyr Thr 610 615 620 Val Thr Leu His Gly Glu Val Arg Gly His Ser Thr Arg Pro Leu Ala 625 630 635 640 Val Glu Val Val Thr Ala Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln 645 650 655 Lys Ile Glu Trp His Glu Gly Thr His His His His His His 660 665 670 < 210 > 102 < 211 > 2013 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 cyA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7478 ( DNA ) < 400 > 102 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa 120 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat 180 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac 240 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg 300 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt 360 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa 420 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat 480 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa 540 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca 600 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat 660 ggttcaacta gtggttctgg tcatcaccat caccatcact ccgcgggtct ggtgccacgc 720 ggtagtactg caattggtat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg 780 gacagcccag atctgggtac cggtggtggc tccggtattg agggacgcgg gtccatggga 840 tatcggggat ccgagctgga cacccccaag gacctgcagg tgtccgagac agccgagaca 900 agcctgaccc tgctgtggaa aacccccctg gccaagttcg accggtacag actgaactac 960 agcctgccca ctggacagtg ggtcggcgtg cagctgcccc ggaacaccac ctcctacgtg 1020 ctgcggggcc tggaacccgg ccaggaatac aacgtcctgc tgacggccga gaagggcaga 1080 cacaagagca agcccgccag agtgaaggcc agcaccgagc aggcccccga gctggaaaac 1140 ctgaccgtga ccgaagtggg ctgggacggc ctgagactga actggacagc cgccgaccag 1200 gcttatgagc acttcgtgat ccaggtgcag gaagccaaca aggtcgaggc cgcccagaat 1260 ctgaccgtgc ccggcaacct gagagccgtg gacatccctg gcctgaaggc tgccaccccc 1320 tacaccgtgt ccatctacgg cgtgattcag ggctaccgga cccctgtgct gtctgccgag 1380 gccagcacag gcgagacacc caacctgggc gaagtgatgg tgtctgaagt gggatgggat 1440 gccctgaagc tcaattggac cgtgcctgag ggcgcctacg agtacttctt tattcaggtc 1500 caggaagctg acaccgtcga agccgctcag aaccacacag tgcctggcgg cctgagaagc 1560 accgatctgc ccggcctgaa agccgctaca cactacacaa tcaccatcag aggcgtgacc 1620 caggacttca gcaccacccc cctgagcgtg gaggtgctga ccgaagaggt acccgacatg 1680 ggcaacctga ccgtgaccga ggtgtcctgg gacgccctgc ggctgaactg gaccaccccc 1740 gacggcacct acgaccagtt cacaatccag gtgcaggaag ccgaccaggt cgaagaggcc 1800 cacaatctga cagtgccagg cagcctgcgg agcatggaaa tccccggcct gagagccggc 1860 accccctaca ccgtgaccct gcacggcgaa gtgcggggcc acagcaccag acccctggcc 1920 gtggaagtgg tcacagctag cggcctgaac gacatcttcg aggctcagaa aatcgaatgg 1980 cacgaaggta cccatcacca tcaccaccac taa 2013 < 210 > 103 < 211 > 670 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 muA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7479 < 400 > 103 Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 1 5 10 15 Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 20 25 30 Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 35 40 45 Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 50 55 60 Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 65 70 75 80 Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 85 90 95 Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 100 105 110 Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 115 120 125 Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 130 135 140 Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 145 150 155 160 Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 165 170 175 Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 180 185 190 Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 195 200 205 Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser 210 215 220 Gly Ser Gly His His His His His His Ser Ala Gly Leu Val Pro Arg 225 230 235 240 Gly Ser Thr Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu 245 250 255 Arg Gln His Met Asp Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly 260 265 270 Ile Glu Gly Arg Gly Ser Met Gly Tyr Arg Gly Ser Glu Leu Asp Thr 275 280 285 Pro Lys Asp Leu Gln Val Ser Glu Thr Ala Glu Thr Ser Leu Thr Leu 290 295 300 Leu Trp Lys Thr Pro Leu Ala Lys Phe Asp Arg Tyr Arg Leu Asn Tyr 305 310 315 320 Ser Leu Pro Thr Gly Gln Trp Val Gly Val Gln Leu Pro Arg Asn Thr 325 330 335 Thr Ser Tyr Val Leu Arg Gly Leu Glu Pro Gly Gln Glu Tyr Asn Val 340 345 350 Leu Leu Thr Ala Glu Lys Gly Arg His Lys Ser Lys Pro Ala Arg Val 355 360 365 Lys Ala Ser Thr Glu Glu Val Pro Ser Leu Glu Asn Leu Thr Val Thr 370 375 380 Glu Ala Gly Trp Asp Gly Leu Arg Leu Asn Trp Thr Ala Asp Asp Leu 385 390 395 400 Ala Tyr Glu Tyr Phe Val Ile Gln Val Gln Glu Ala Asn Asn Val Glu 405 410 415 Thr Ala His Asn Phe Thr Val Pro Gly Asn Leu Arg Ala Ala Asp Ile 420 425 430 Pro Gly Leu Lys Val Ala Thr Ser Tyr Arg Val Ser Ile Tyr Gly Val 435 440 445 Ala Arg Gly Tyr Arg Thr Pro Val Leu Ser Ala Glu Thr Ser Thr Gly 450 455 460 Thr Thr Pro Asn Leu Gly Glu Val Thr Val Ala Glu Val Gly Trp Asp 465 470 475 480 Ala Leu Thr Leu Asn Trp Thr Ala Pro Glu Gly Ala Tyr Lys Asn Phe 485 490 495 Phe Ile Gln Val Leu Glu Ala Asp Thr Thr Gln Thr Val Gln Asn Leu 500 505 510 Thr Val Pro Gly Gly Leu Arg Ser Val Asp Leu Pro Gly Leu Lys Ala 515 520 525 Ala Thr Arg Tyr Tyr Ile Thr Leu Arg Gly Val Thr Gln Asp Phe Gly 530 535 540 Thr Ala Pro Leu Ser Val Glu Val Leu Thr Glu Glu Val Pro Asp Met 545 550 555 560 Gly Asn Leu Thr Val Thr Glu Val Ser Trp Asp Ala Leu Arg Leu Asn 565 570 575 Trp Thr Thr Pro Asp Gly Thr Tyr Asp Gln Phe Thr Ile Gln Val Gln 580 585 590 Glu Ala Asp Gln Val Glu Glu Ala His Asn Leu Thr Val Pro Gly Ser 595 600 605 Leu Arg Ser Met Glu Ile Pro Gly Leu Arg Ala Gly Thr Pro Tyr Thr 610 615 620 Val Thr Leu His Gly Glu Val Arg Gly His Ser Thr Arg Pro Leu Ala 625 630 635 640 Val Glu Val Val Thr Ala Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln 645 650 655 Lys Ile Glu Trp His Glu Gly Thr His His His His His His 660 665 670 < 210 > 104 < 211 > 2013 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > TNC Fn 5 muA 1 A 2 huA 3 avi his biotin from E . coli = pETR 7479 ( DNA ) < 400 > 104 atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt 60 ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa 120 tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat 180 ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac 240 atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg 300 gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt 360 gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa 420 acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat 480 gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa 540 aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca 600 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat 660 ggttcaacta gtggttctgg tcatcaccat caccatcact ccgcgggtct ggtgccacgc 720 ggtagtactg caattggtat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg 780 gacagcccag atctgggtac cggtggtggc tccggtattg agggacgcgg gtccatggga 840 tatcggggat ccgagctgga cacccccaag gacctgcagg tgtccgagac agccgagaca 900 agcctgaccc tgctgtggaa aacccccctg gccaagttcg accggtacag actgaactac 960 agcctgccca ctggacagtg ggtcggcgtg cagctgcccc ggaacaccac ctcctacgtg 1020 ctgcggggcc tggaacccgg ccaggaatac aacgtcctgc tgacggccga gaagggccgg 1080 cacaagagca agcccgccag agtgaaggcc agcaccgagg aagtgcccag cctggaaaac 1140 ctgaccgtga ccgaggccgg ctgggacggc ctgcggctga actggaccgc cgacgacctg 1200 gcctacgagt acttcgtgat ccaggtgcag gaagccaaca acgtcgagac agcccacaac 1260 ttcaccgtgc ccggcaacct gagagccgcc gacatccccg gcctgaaggt ggccacatcc 1320 taccgggtgt ccatctacgg cgtggccagg ggctaccgga cccccgtgct gtccgccgag 1380 acaagcaccg gcaccacgcc gaacctgggc gaagtgaccg tggcggaagt gggttgggat 1440 gcgctgaccc tgaattggac cgcaccggaa ggcgcgtata aaaacttttt catccaggtg 1500 ctggaagcgg ataccaccca gaccgtgcag aacctgaccg tgccgggtgg tctgcgtagc 1560 gtggatctgc cgggcctgaa agcggcgacc cgttattaca ttaccctgcg tggcgtgacc 1620 caggattttg gcaccgcgcc gctgtctgtg gaagtgctga ccgaggaggt acccgacatg 1680 ggcaacctga ccgtgaccga ggtgtcctgg gacgccctgc ggctgaactg gaccaccccc 1740 gacggcacct acgaccagtt cacaatccag gtgcaggaag ccgaccaggt cgaagaggcc 1800 cacaatctga cagtgccagg cagcctgcgg agcatggaaa tccccggcct gagagccggc 1860 accccctaca ccgtgaccct gcacggcgaa gtgcggggcc acagcaccag acccctggcc 1920 gtggaagtgg tcacagctag cggcctgaac gacatcttcg aggctcagaa aatcgaatgg 1980 cacgaaggta cccatcacca tcaccaccac taa 2013 < 210 > 105 < 211 > 773 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > HEK human TNC 1 - 8 < 400 > 105 Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 1 5 10 15 Val His Ser Glu Val Ser Pro Pro Lys Asp Leu Val Val Thr Glu Val 20 25 30 Thr Glu Glu Thr Val Asn Leu Ala Trp Asp Asn Glu Met Arg Val Thr 35 40 45 Glu Tyr Leu Val Val Tyr Thr Pro Thr His Glu Gly Gly Leu Glu Met 50 55 60 Gln Phe Arg Val Pro Gly Asp Gln Thr Ser Thr Ile Ile Arg Glu Leu 65 70 75 80 Glu Pro Gly Val Glu Tyr Phe Ile Arg Val Phe Ala Ile Leu Glu Asn 85 90 95 Lys Lys Ser Ile Pro Val Ser Ala Arg Val Ala Thr Tyr Leu Pro Ala 100 105 110 Pro Glu Gly Leu Lys Phe Lys Ser Ile Lys Glu Thr Ser Val Glu Val 115 120 125 Glu Trp Asp Pro Leu Asp Ile Ala Phe Glu Thr Trp Glu Ile Ile Phe 130 135 140 Arg Asn Met Asn Lys Glu Asp Glu Gly Glu Ile Thr Lys Ser Leu Arg 145 150 155 160 Arg Pro Glu Thr Ser Tyr Arg Gln Thr Gly Leu Ala Pro Gly Gln Glu 165 170 175 Tyr Glu Ile Ser Leu His Ile Val Lys Asn Asn Thr Arg Gly Pro Gly 180 185 190 Leu Lys Arg Val Thr Thr Thr Arg Leu Asp Ala Pro Ser Gln Ile Glu 195 200 205 Val Lys Asp Val Thr Asp Thr Thr Ala Leu Ile Thr Trp Phe Lys Pro 210 215 220 Leu Ala Glu Ile Asp Gly Ile Glu Leu Thr Tyr Gly Ile Lys Asp Val 225 230 235 240 Pro Gly Asp Arg Thr Thr Ile Asp Leu Thr Glu Asp Glu Asn Gln Tyr 245 250 255 Ser Ile Gly Asn Leu Lys Pro Asp Thr Glu Tyr Glu Val Ser Leu Ile 260 265 270 Ser Arg Arg Gly Asp Met Ser Ser Asn Pro Ala Lys Glu Thr Phe Thr 275 280 285 Thr Gly Leu Asp Ala Pro Arg Asn Leu Arg Arg Val Ser Gln Thr Asp 290 295 300 Asn Ser Ile Thr Leu Glu Trp Arg Asn Gly Lys Ala Ala Ile Asp Ser 305 310 315 320 Tyr Arg Ile Lys Tyr Ala Pro Ile Ser Gly Gly Asp His Ala Glu Val 325 330 335 Asp Val Pro Lys Ser Gln Gln Ala Thr Thr Lys Thr Thr Leu Thr Gly 340 345 350 Leu Arg Pro Gly Thr Glu Tyr Gly Ile Gly Val Ser Ala Val Lys Glu 355 360 365 Asp Lys Glu Ser Asn Pro Ala Thr Ile Asn Ala Ala Thr Glu Leu Asp 370 375 380 Thr Pro Lys Asp Leu Gln Val Ser Glu Thr Ala Glu Thr Ser Leu Thr 385 390 395 400 Leu Leu Trp Lys Thr Pro Leu Ala Lys Phe Asp Arg Tyr Arg Leu Asn 405 410 415 Tyr Ser Leu Pro Thr Gly Gln Trp Val Gly Val Gln Leu Pro Arg Asn 420 425 430 Thr Thr Ser Tyr Val Leu Arg Gly Leu Glu Pro Gly Gln Glu Tyr Asn 435 440 445 Val Leu Leu Thr Ala Glu Lys Gly Arg His Lys Ser Lys Pro Ala Arg 450 455 460 Val Lys Ala Ser Thr Glu Gln Ala Ala Met Gly Ser Pro Lys Glu Val 465 470 475 480 Ile Phe Ser Asp Ile Thr Glu Asn Ser Ala Thr Val Ser Trp Arg Ala 485 490 495 Pro Thr Ala Gln Val Glu Ser Phe Arg Ile Thr Tyr Val Pro Ile Thr 500 505 510 Gly Gly Thr Pro Ser Met Val Thr Val Asp Gly Thr Lys Thr Gln Thr 515 520 525 Arg Leu Val Lys Leu Ile Pro Gly Val Glu Tyr Leu Val Ser Ile Ile 530 535 540 Ala Met Lys Gly Phe Glu Glu Ser Glu Pro Val Ser Gly Ser Phe Thr 545 550 555 560 Thr Ala Leu Asp Gly Pro Ser Gly Leu Val Thr Ala Asn Ile Thr Asp 565 570 575 Ser Glu Ala Leu Ala Arg Trp Gln Pro Ala Ile Ala Thr Val Asp Ser 580 585 590 Tyr Val Ile Ser Tyr Thr Gly Glu Lys Val Pro Glu Ile Thr Arg Thr 595 600 605 Val Ser Gly Asn Thr Val Glu Tyr Ala Leu Thr Asp Leu Glu Pro Ala 610 615 620 Thr Glu Tyr Thr Leu Arg Ile Phe Ala Glu Lys Gly Pro Gln Lys Ser 625 630 635 640 Ser Thr Ile Thr Ala Lys Phe Thr Thr Asp Leu Asp Ser Pro Arg Asp 645 650 655 Leu Thr Ala Thr Glu Val Gln Ser Glu Thr Ala Leu Leu Thr Trp Arg 660 665 670 Pro Pro Arg Ala Ser Val Thr Gly Tyr Leu Leu Val Tyr Glu Ser Val 675 680 685 Asp Gly Thr Val Lys Glu Val Ile Val Gly Pro Asp Thr Thr Ser Tyr 690 695 700 Ser Leu Ala Asp Leu Ser Pro Ser Thr His Tyr Thr Ala Lys Ile Gln 705 710 715 720 Ala Leu Asn Gly Pro Leu Arg Ser Asn Met Ile Gln Thr Ile Phe Thr 725 730 735 Thr Val Asp Leu Glu Val Leu Phe Gln Gly Pro Gly Ser Gly Leu Asn 740 745 750 Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu Ala Arg Ala His 755 760 765 His His His His His 770 < 210 > 106 < 211 > 2322 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > HEK human TNC 1 - 8 ( DNA ) < 400 > 106 atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattccgag 60 gtgtctcctc ccaaagacct cgttgtgaca gaagtgacgg aagagacggt caacctggcc 120 tgggacaatg agatgcgggt cacagagtac cttgtcgtgt acacgcccac ccacgagggt 180 ggtctggaaa tgcagttccg tgtgcctggg gaccagacgt ccaccatcat ccgggagctg 240 gagcctggtg tggagtactt tatccgtgta tttgccatcc tggagaacaa gaagagcatt 300 cctgtcagcg ccagggtggc cacgtactta cctgcacctg aaggcctgaa attcaagtcc 360 atcaaggaga catctgtgga agtggagtgg gatcctctag acattgcttt tgaaacctgg 420 gagatcatct tccggaatat gaataaagaa gatgagggag agatcaccaa aagcctgagg 480 aggccagaga cctcttaccg gcaaactggt ctagctcctg ggcaagagta tgagatatct 540 ctgcacatag tgaaaaacaa tacccggggc cctggcctga agagggtgac caccacacgc 600 ttggatgccc ccagccagat cgaggtgaaa gatgtcacag acaccactgc cttgatcacc 660 tggttcaagc ccctggctga gatcgatggc attgagctga cctacggcat caaagacgtg 720 ccaggagacc gtaccaccat cgatctcaca gaggacgaga accagtactc catcgggaac 780 ctgaagcctg acactgagta cgaggtgtcc ctcatctccc gcagaggtga catgtcaagc 840 aacccagcca aagagacctt cacaacaggc ctcgatgctc ccaggaatct tcgacgtgtt 900 tcccagacag ataacagcat caccctggaa tggaggaatg gcaaggcagc tattgacagt 960 tacagaatta agtatgcccc catctctgga ggggaccacg ctgaggttga tgttccaaag 1020 agccaacaag ccacaaccaa aaccacactc acaggtctga ggccgggaac tgaatatggg 1080 attggagttt ctgctgtgaa ggaagacaag gagagcaatc cagcgaccat caacgcagcc 1140 acagagttgg acacgcccaa ggaccttcag gtttctgaaa ctgcagagac cagcctgacc 1200 ctgctctgga agacaccgtt ggccaaattt gaccgctacc gcctcaatta cagtctcccc 1260 acaggccagt gggtgggagt gcagcttcca agaaacacca cttcctatgt cctgagaggc 1320 ctggaaccag gacaggagta caatgtcctc ctgacagccg agaaaggcag acacaagagc 1380 aagcccgcac gtgtgaaggc atccactgaa caagccgcca tgggctcccc aaaggaagtc 1440 attttctcag acatcactga aaattcggct actgtcagct ggagggcacc cacagcccaa 1500 gtggagagct tccggattac ctatgtgccc attacaggag gtacaccctc catggtaact 1560 gtggacggaa ccaagactca gaccaggctg gtgaaactca tacctggcgt ggagtacctt 1620 gtcagcatca tcgccatgaa gggctttgag gaaagtgaac ctgtctcagg gtcattcacc 1680 acagctctgg atggcccatc tggcctggtg acagccaaca tcactgactc agaagccttg 1740 gccaggtggc agccagccat tgccactgtg gacagttatg tcatctccta cacaggcgag 1800 aaagtgccag aaattacacg cacggtgtcc gggaacacag tggagtatgc tctgaccgac 1860 ctcgagcctg ccacggaata cacactgaga atctttgcag agaaagggcc ccagaagagc 1920 tcaaccatca ctgccaagtt cacaacagac ctcgattctc caagagactt gactgctact 1980 gaggttcagt cggaaactgc cctccttacc tggcgacccc cccgggcatc agtcaccggt 2040 tacctgctgg tctatgaatc agtggatggc acagtcaagg aagtcattgt gggtccagat 2100 accacctcct acagcctggc agacctgagc ccatccaccc actacacagc caagatccag 2160 gcactcaatg ggcccctgag gagcaatatg atccagacca tcttcaccac agtcgacctg 2220 gaagttctgt tccaggggcc cggctcaggc ctgaacgaca tcttcgaggc ccagaagatc 2280 gagtggcacg aggctcgagc tcaccaccat caccatcact ga 2322 < 210 > 107 < 211 > 19 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 1 < 400 > 107 Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 1 5 10 15 Ala His Ser < 210 > 108 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 1 ( DNA 1 ) < 400 > 108 atggactgga cctggagaat cctcttcttg gtggcagcag ccacaggagc ccactcc 57 < 210 > 109 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 1 ( DNA 2 ) < 400 > 109 atggactgga cctggaggat cctcttcttg gtggcagcag ccacaggagc ccactcc 57 < 210 > 110 < 211 > 22 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 2 < 400 > 110 Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 1 5 10 15 Phe Pro Gly Ala Arg Cys 20 < 210 > 111 < 211 > 66 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 2 ( DNA ) < 400 > 111 atggacatga gggtccccgc tcagctcctg ggcctcctgc tgctctggtt cccaggtgcc 60 aggtgt 66 < 210 > 112 < 211 > 19 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 3 < 400 > 112 Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 1 5 10 15 Val His Ser < 210 > 113 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 3 ( DNA 1 ) < 400 > 113 atgggatgga gctgtatcat cctcttcttg gtagcaacag ctaccggtgt gcattcc 57 < 210 > 114 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 3 ( DNA 1 ) < 400 > 114 atgggctggt cctgcatcat cctgtttctg gtggctaccg ccactggagt gcattcc 57 < 210 > 115 < 211 > 57 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Leader Sequence 3 ( DNA 3 ) < 400 > 115 atgggctggt cctgcatcat cctgtttctg gtcgccacag ccaccggcgt gcactct 57 < 210 > 116 < 211 > 26 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LMB 3 < 400 > 116 caggaaacag ctatgaccat gattac 26 < 210 > 117 < 211 > 63 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LibL 1 b _ new < 220 > < 221 > misc _ feature < 222 > ( 26 ) . .( 27 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 32 ) . .( 33 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 35 ) . .( 36 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 38 ) . .( 39 ) < 223 > n is a , c , g , or t < 400 > 117 cactttggtc ccctggccga acgtmnnggg mnnmnnmnna ccctgctgac agtaatacac 60 tgc 63 < 210 > 118 < 211 > 28 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > MS 63 < 400 > 118 tttcgcacag taatatacgg ccgtgtcc 28 < 210 > 119 < 211 > 25 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > MS 64 < 400 > 119 acgttcggcc aggggaccaa agtgg 25 < 210 > 120 < 211 > 60 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > Lib 2 H < 220 > < 221 > misc _ feature < 222 > ( 23 ) . .( 24 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 26 ) . .( 27 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 29 ) . .( 30 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 32 ) . .( 33 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 35 ) . .( 36 ) < 223 > n is a , c , g , or t < 400 > 120 ggccgtatat tactgtgcga aannknnknn knnknnkttt gactactggg gccaaggaac 60 < 210 > 121 < 211 > 30 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > fdseqlong < 400 > 121 gacgttagta aatgaatttt ctgtatgagg 30 < 210 > 122 < 211 > 69 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH _ LIB 3 < 220 > < 221 > misc _ feature < 222 > ( 26 ) . .( 27 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 32 ) . .( 33 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 35 ) . .( 36 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 41 ) . .( 42 ) < 223 > n is a , c , g , or t < 400 > 122 gactttggtg ccctggccaa acgtmnnggg mnnmnnaccm nnctgcaagc agtaataggt 60 ggcaaaatc 69 < 210 > 123 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 31 < 400 > 123 acgtttggcc agggcaccaa agtcgag 27 < 210 > 124 < 211 > 28 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 32 < 400 > 124 tctcgcacag taatacacgg cggtgtcc 28 < 210 > 125 < 211 > 94 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > LIB 88 _ 2 < 220 > < 221 > misc _ difference < 222 > ( 29 ) . .( 55 ) < 223 > ( 33 % GAC Asp ; 26 % GGT Gly ; 10 % GAA Glu ; 9 % CGT Arg ; 7 % Lys ; 6 % GTT Val ; 5 % TCT Ser ; 4 % CTG Leu ) 1 - ( 23 % GGT Gly ; 17 % TAC Tyr ; 16 % TCT Ser ; 11 % GCT Ala ; 9 % CGT Arg ; 7 % AAC Asn ; 6 % ACT Thr ; 6 % GTT Val ; 5 % CCG Pro ) 8 < 220 > < 221 > misc _ feature < 222 > ( 30 ) . .( 33 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 35 ) . .( 36 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 38 ) . .( 39 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 41 ) . .( 42 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 44 ) . .( 45 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 47 ) . .( 48 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 50 ) . .( 51 ) < 223 > n is a , c , g , or t < 220 > < 221 > misc _ feature < 222 > ( 53 ) . .( 54 ) < 223 > n is a , c , g , or t < 400 > 125 ggacaccgcc gtgtattact gtgcgagabn nnnbnnbnnb nnbnnbnnbn nbnnbtttga 60 ctactggggc caagggacca ccgtgaccgt ctcc 94 < 210 > 126 < 211 > 63 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > AM _ Vk 1 A 30 _ L 1 _ ba < 400 > 126 cctggcttct gctggtacca gcctaaatca ttacgaatgc cctgacttgc ccggcaggtg 60 atg 63 < 210 > 127 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 50 ( Vk 1 A 30 _ L 1 / L 2 _ fo ) < 400 > 127 gctggtacca gcagaagcca gggaaag 27 < 210 > 128 < 211 > 26 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 51 ( Vk 1 A 30 _ BsiWI _ ba ) < 400 > 128 ggtgcagcca ccgtacgctt gatctc 26 < 210 > 129 < 211 > 61 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > AM _ Vk 1 A 30 _ L 2 _ ba < 400 > 129 cttgatggga cgccactctg caaactggac gcagcataga tcaggcgctt aggggctttc 60 c 61 < 210 > 130 < 211 > 27 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 52 ( Vk 1 A 30 _ L 2 / L 3 ) < 400 > 130 ttgcagagtg gcgtcccatc aaggttc 27 < 210 > 131 < 211 > 26 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 53 < 400 > 131 catcagggcc tgagctcgcc cgtcac 26 < 210 > 132 < 211 > 62 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > AM _ DP 88 _ H 1 _ ba _ opt < 400 > 132 gtccaggggc ctgtcgcacc cagcttatag cgtagctgct gaatgtgcct ccggaggcct 60 tg 62 < 210 > 133 < 211 > 28 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 54 ( DP 88 _ H 1 / H 2 _ fo ) < 400 > 133 ataagctggg tgcgacaggc ccctggac 28 < 210 > 134 < 211 > 26 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > MS 52 < 400 > 134 gaagaccgat gggcctttgg tgctag 26 < 210 > 135 < 211 > 83 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > AM _ DP 88 _ H 2 _ ba < 400 > 135 gaccctgccc tggaacttct gtgcgtagtt tgcggtacca aagataggga tgatccctcc 60 catccactcg agcccttgtc cag 83 < 210 > 136 < 211 > 29 < 212 > DNA < 213 > Artificial Sequence < 220 > < 223 > RJH 55 ( DP 88 _ H 2 H 3 _ fo ) < 400 > 136 tacgcacaga agttccaggg cagggtcac 29