휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - Flag 태그 | S inoBiologicalRegisterSign in 고로 그 아웃 내 계정 Bulk Order 장바구니 ( 0 ) Contact UsAfter search , choose a molecule or a kind of categories listed in the left to narrow down your filter . If you have any problems , please contact us ！ ProteinantibodyGene / cDNA qPCR PrimerELISA kitsTools 사이트 내 검색 AllRecombinant ProteinAntibodyELISA Pair SetcDNA ORF CloneELISA KitqPCRother 제품 검색 한국어 EnglishChinese ( 中文 简 体 ) Japanese ( 日本 语 ) German ( Deutsch ) French ( Francais ) Korean ( 한국어 ) Spanish ( Español ) Portuguese ( português ) Russian ( русский ) Chinese ( 中文 繁 體 ) 제품 유형 빠른 주문 장바구니 에 추가 제품 Recombinant Proteins ▷ Human Protein ▷ Mouse Protein ▷ Rat Protein ▷ Cynomolgus Protein ▷ Canine Protein ▷ Influenza Protein ▷ Virus Protein ▷ Other Protein 항체 ▷ Primary Antibody 
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 BGH ( TAGAAGGCACAGTCGAGG ) Promoter : Enhanced CMV mammalian cell promoterApplication : Stable or Transient mammalian expressionAntibiotic in E . coli : KanamycinAntibiotic in mammalian cell : HygromycinShipping _ carrier : Each tube contains lyophilized plasmid . 보관 : The lyophilized plasmid can be stored at room temperature for three months . IDO 1 cDNA 클론 염기 순서 및 품질 관리 Yellow ： linkerGreen ： FLAG tag A T G G C A C A C G C T A T G G A A A A C T C C T G G A C A A T C A G T A A A G A G T A C C A T A T T G A T G A A G A A G T G G G C T T T G C T C T G C C A A A T C C A C A G G A A A A T C T A C C T G A T T T T T A T A A T G A C T G G A T G T T C A T T G C T A A A C A T C T G C C T G A T C T C A T A G A G T C T G G C C A G C T T C G A G A A A G A G T T G A G A A G T T A A A C A T G C T C A G C A T T G A T C A T C T C A C A G A C C A C A A G T C A C A G C G C C T T G C A C G T C T A G T T C T G G G A T G C A T C A C C A T G G C A T A T G T G T G G G G C A A A G G T C A T G G A G A T G T C C G T A A G G T C T T G C C A A G A A A T A T T G C T G T T C C T T A C T G C C A A C T C T C C A A G A A A C T G G A A C T G C C T C C T A T T T T G G T T T A T G C A G A C T G T G T C T T G G C A A A C T G G A A G A A A A A G G A T C C T A A T A A G C C C C T G A C T T A T G A G A A C A T G G A C G T T T T G T T C T C A T T T C G T G A T G G A G A C T G C A G T A A A G G A T T C T T C C T G G T C T C T C T A T T G G T G G A A A T A G C A G C T G C T T C T G C A A T C A A A G T A A T T C C T A C T G T A T T C A A G G C A A T G C A A A T G C A A G A A C G G G A C A C T T T G C T A A A G G C G C T G T T G G A A A T A G C T T C T T G C T T G G A G A A A G C C C T T C A A G T G T T T C A C C A A A T C C A C G A T C A T G T G A A C C C A A A A G C A T T T T T C A G T G T T C T T C G C A T A T A T T T G T C T G G C T G G A A A G G C A A C C C C C A G C T A T C A G A C G G T C T G G T G T A T G A A G G G T T C T G G G A A G A C C C A A A G G A G T T T G C A G G G G G C A G T G C A G G C C A A A G C A G C G T C T T T C A G T G C T T T G A C G T C C T G C T G G G C A T C C A G C A G A C T G C T G G T G G A G G A C A T G C T G C T C A G T T C C T C C A G G A C A T G A G A A G A T A T A T G C C A C C A G C T C A C A G G A A C T T C C T G T G C T C A T T A G A G T C A A A T C C C T C A G T C C G T G A G T T T G T C C T T T C A A A A G G T G A T G C T G G C C T G C G G G A A G C T T A T G A C G C C T G T G T G A A A G C T C T G G T C T C C C T G A G G A G C T A C C A T C T G C A A A T C G T G A C T A A G T A C A T C C T G A T T C C T G C A A G C C A G C A G C C A A A G G A G A A T A A G A C C T C T G A A G A C C C T T C A A A A C T G G A A G C C A A A G G A A C T G G A G G C A C T G A T T T A A T G A A T T T C C T G A A G A C T G T A A G A A G T A C A A C T G A G A A A T C C C T T T T G A A G G A A G G T G G G G G T G G A G G C T C T G A TTACAAGGATGACGACGATAAGTAA M A H A M E N S W T I S K E Y H I D E E V G F A L P N P Q E N L P D F Y N D W M F I A K H L P D L I E S G Q L R E R V E K L N M L S I D H L T D H K S Q R L A R L V L G C I T M A Y V W G K G H G D V R K V L P R N I A V P Y C Q L S K K L E L P P I L V Y A D C V L A N W K K K D P N K P L T Y E N M D V L F S F R D G D C S K G F F L V S L L V E I A A A S A I K V I P T V F K A M Q M Q E R D T L L K A L L E I A S C L E K A L Q V F H Q I H D H V N P K A F F S V L R I Y L S G W K G N P Q L S D G L V Y E G F W E D P K E F A G G S A G Q S S V F Q C F D V L L G I Q Q T A G G G H A A Q F L Q D M R R Y M P P A H R N F L C S L E S N P S V R E F V L S K G D A G L R E A Y D A C V K A L V S L R S Y H L Q I V T K Y I L I P A S Q Q P K E N K T S E D P S K L E A K G T G G T D L M N F L K T V RSTTEKSLLKEGGGGGSDYKDDDDK - Human IDO 1 Gene Plasmid Map [ 이미지 를 확대 하 려면 클릭 ] Human IDO 1 natural ORF mammalian expression plasmid , C - Flag tagHuman IDO 1 Gene Expression validated Image [ 이미지 를 확대 하 려면 클릭 ] Caption : The plasmid was transfected into 293 H adherent cells with Sinofection reagent ( Cat # STF 01 ) . After 48 h , Immunofluorescence staining of cells . Cells were fixed with 4 % PFA , permeabilzed with 0 . 3 % Triton X - 100 in PBS , blocked with 10 % serum , and incubated with Mouse anti - Flag Tag monoclonal antibody ( CST # 8146 S ) at 37 ℃ 1 hour . Then cells were stained with Goat Anti - mouse IgG secondary antibody . The fluorescent signal is detected by fluorescence microscope . Each expression experiment has negative control . 
 FLAG - tag , or FLAG octapeptide , is a polypeptide protein tag that can be added to a protein using recombinant DNA technology . It can be used for affinity chromatography , then used to separate recombinant , overexpressed protein from wild - type protein expressed by the host organism . It can also be used in the isolation of protein complexes with multiple subunits . A FLAG - tag can be used in many different assays that require recognition by an antibody . If there is no antibody against the studied protein , adding a FLAG - tag to this protein allows one to follow the protein with an antibody against the FLAG sequence . Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis . The peptide sequence of the FLAG - tag from the N - terminus to the C - terminus is : DYKDDDDK ( 1012 Da ) . It can be used in conjunction with other affinity tags , for example a polyhistidine tag ( His - tag ) , HA - tag or Myc - tag . It can be fused to the C - terminus or the N - terminus of a protein . Some commercially available antibodies ( e . g . , M 1 / 4 E 11 ) recognize the epitope only when it is present at the N - terminus . However , other available antibodies ( e . g . , M 2 ) are position - insensitive . 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - Flag 태그 on other vectors 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - GFPSpark 태그 HG 11650 - ACG ₩ 265260 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - OFPSpark 태그 HG 11650 - ACR ₩ 265260 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - GFPSpark 태그 HG 11650 - ANG ₩ 265260 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - OFPSpark 태그 HG 11650 - ANR ₩ 265260 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - Flag 태그 HG 11650 - CF ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - His 태그 HG 11650 - CH ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - Myc 태그 HG 11650 - CM ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - HA 태그 HG 11650 - CY ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone in cloning vectorHG 11650 - M ₩ 88420 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - Flag 태그 HG 11650 - NF ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - His 태그 HG 11650 - NH ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - Myc 태그 HG 11650 - NM ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , N - HA 태그 HG 11650 - NY ₩ 229890 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmidHG 11650 - UT ₩ 229890 발현 벡터 에 대해 자세히 알아보 기 Proteins Antibodies ELISA KitsProduct nameProduct nameProduct nameProduct nameProduct nameProduct name 연구 배경 Indoleamine 2 , 3 - dioxygenase - 1 , also known as Indoleamine - pyrrole 2 , 3 - dioxygenase , IDO 1 and IDO , is a member of the indoleamine 2 , 3 - dioxygenase family . IDO 1 / IDO and tryptophan 2 , 3 - dioxygenase ( TDO ) are tryptophan - degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway . TDO is widely distributed in both eukaryotes and bacteria . In contrast , IDO has been found only in mammals and yeast . In 2007 , a third enzyme , indoleamine 2 , 3 - dioxygenase - 2 ( IDO 2 ) , was discovered . IDO 2 is found not only in mammals but also in lower vertebrates . IDO 1 / IDO is an immunosuppressive molecule inducible in various cells . IDO 1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen . It mediates oxidative cleavage of tryptophan , an amino acid essential for cell proliferation and survival . IDO 1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency - associated abnormalities , including cancer . The IDO pathway is activated in multiple tumor types . Selective inhibition of IDO 1 may represent an attractive cancer therapeutic strategy via up - regulation of cellular immunity . IDO 1 / IDO is an enzyme that suppresses adaptive T - cell immunity by catabolizing tryptophan from the cellular microenvironment . Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer . 참고 자료 
 Size / PriceCat No : HG 11650 - CF 정가 : 가격 : ( You Save : ) 크기 : 수량 : +- 재고 정보 In Stock 대량 주문 문 의 장바구니 에 추가 Contact Us 
 Email : order @ sinobiological . comsee our distributors 이미지 최근 에 조회 한 품목 휴먼 IDO 1 / Indoleamine 2 , 3 ‑ dioxygenase Gene ORF cDNA clone expression plasmid , C - Flag 태그 Recommended ProductsSinofection Transfection Reagent 0 - 9 A B C D EFGHIJKLMNOPQRSTUVWXYZAll information of our products is subject to change without notice . Please refer to COA enclosed in shipped package for the newest information . 주의 : 모든 제품 은 " 연구 목적 만 을 위한 것 이 며 진단 이나 치료 에 사용 하 도록 의도 되 지 않 았 습니다 ". 제품 제품 유형 별 단백질 항체 ELISA 분석 개발 서비스 cDNA 클론 트 렌 스펙 션 시약 재조합 효소 항체 정제 Antibody isotyping kit 연구 주제 별 암 발생 생물학 효소 후성 유전학 면역학 신경 과학 신호 전달 줄기세포 서비스 항체 개발 및 생산 단백질 발현 및 생산 하이브리드 도마 원 스톱 서비스 안정 적 세포주 개발 바이오 분석 평가 서비스 도움 말 및 지원 주문 방법 결제 지침 배송 정보 기술 FAQ 의 문 사항 이 있 으시 면 메시지 를 남겨 주 십시오 . 연락처 order @ sinobiological . com + 86 - 400 - 890 - 9989 Sino Biological 팔 로우 하 기 : 微 信服 务 号 ： 义 翘 神 州 生物 조항 개인 정보 보호 정책 법무 사이트 맵 친구 링크 © 2007 - 2016 Sino Biological Inc . All rights reservedTop