1 MRS agar + NaN 3 0 . 02 % + L - cysteine hydrecloride monohydrate 0 . 05 % ( anaerobic ) . 2 MRS agar + NaN 3 0 . 02 % ( aerobic ) . 3 TSC agar + Egg yolk emulsion ( Distilled water : egg yolk = 4 : 1 ) 25 ml / 500 ml + cysloserine ( 1 mg / 500 ml ) * Anaerobic : Gas pak anaerobic system ( BBL ) .