< 110 > PROGEN CO . , LTD . Genexine , Inc . < 120 > A novel IL - 10 variant protein and use thereof < 130 > PD 20 - 5948 < 150 > KR 10 - 2019 - 0082149 < 151 > 2019 - 07 - 08 < 160 > 46 < 170 > KoPatentIn 3 . 0 < 210 > 1 < 211 > 169 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > monomeric IL - 10 variant < 400 > 1 Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 1 5 10 15 Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 20 25 30 Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 35 40 45 Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 50 55 60 Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 65 70 75 80 Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu 85 90 95 Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 100 105 110 Pro Cys Glu Asn Gly Gly Ser Gly Gly Ser Gly Gly Ser Lys Ser Lys 115 120 125 Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly 130 135 140 Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu 145 150 155 160 Ala Tyr Met Thr Met Lys Ile Arg Asn 165 < 210 > 2 < 211 > 20 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 2 Ala Ala Gly Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 Gly Gly Gly Ser 20 < 210 > 3 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 3 Gly Gly Ser Gly Gly 1 5 < 210 > 4 < 211 > 9 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > spacer peptide < 400 > 4 Gly Gly Ser Gly Gly Ser Gly Gly Ser 1 5 < 210 > 5 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > spacer peptide < 400 > 5 Gly Gly Gly Ser Gly Gly 1 5 < 210 > 6 < 211 > 214 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > modified Fc region lysine deleted < 400 > 6 Ser His Thr Gln Pro Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys 1 5 10 15 Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 20 25 30 Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 35 40 45 Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 50 55 60 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 65 70 75 80 Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 85 90 95 Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 100 105 110 Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 115 120 125 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 130 135 140 Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 145 150 155 160 Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 165 170 175 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 180 185 190 Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 195 200 205 Leu Ser Leu Ser Leu Gly 210 < 210 > 7 < 211 > 215 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > modified Fc region < 400 > 7 Ser His Thr Gln Pro Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys 1 5 10 15 Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 20 25 30 Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 35 40 45 Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 50 55 60 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 65 70 75 80 Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 85 90 95 Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 100 105 110 Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 115 120 125 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 130 135 140 Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 145 150 155 160 Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 165 170 175 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 180 185 190 Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 195 200 205 Leu Ser Leu Ser Leu Gly Lys 210 215 < 210 > 8 < 211 > 214 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > modified Fc region lysine deleted < 400 > 8 Ser His Thr Gln Pro Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys 1 5 10 15 Asp Gln Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 20 25 30 Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 35 40 45 Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 50 55 60 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 65 70 75 80 Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 85 90 95 Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 100 105 110 Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 115 120 125 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 130 135 140 Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 145 150 155 160 Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 165 170 175 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 180 185 190 Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 195 200 205 Leu Ser Leu Ser Leu Gly 210 < 210 > 9 < 211 > 215 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > modified Fc region < 400 > 9 Ser His Thr Gln Pro Leu Gly Val Phe Leu Phe Pro Pro Lys Pro Lys 1 5 10 15 Asp Gln Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 20 25 30 Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 35 40 45 Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 50 55 60 Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 65 70 75 80 Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 85 90 95 Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 100 105 110 Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 115 120 125 Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 130 135 140 Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 145 150 155 160 Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 165 170 175 Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 180 185 190 Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 195 200 205 Leu Ser Leu Ser Leu Gly Lys 210 215 < 210 > 10 < 211 > 160 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > dimeric IL - 10 I 87 A variant < 400 > 10 Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 1 5 10 15 Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 20 25 30 Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 35 40 45 Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 50 55 60 Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 65 70 75 80 Glu Asn Gln Asp Pro Asp Ala Lys Ala His Val Asn Ser Leu Gly Glu 85 90 95 Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 100 105 110 Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe 115 120 125 Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp 130 135 140 Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 145 150 155 160 < 210 > 11 < 211 > 166 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > prior monomeric IL - 10 I 87 A variant < 400 > 11 Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 1 5 10 15 Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 20 25 30 Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 35 40 45 Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 50 55 60 Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 65 70 75 80 Glu Asn Gln Asp Pro Asp Ala Lys Ala His Val Asn Ser Leu Gly Glu 85 90 95 Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 100 105 110 Pro Cys Glu Asn Gly Gly Gly Ser Gly Gly Lys Ser Lys Ala Val Glu 115 120 125 Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys 130 135 140 Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met 145 150 155 160 Thr Met Lys Ile Arg Asn 165 < 210 > 12 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 12 Gly Gly Gly Gly Ser 1 5 < 210 > 13 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 13 Gly Ser Ser Gly Gly Ser 1 5 < 210 > 14 < 211 > 18 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 14 Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 1 5 10 15 Leu Asp < 210 > 15 < 211 > 14 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 15 Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser Thr 1 5 10 < 210 > 16 < 211 > 12 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 16 Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 1 5 10 < 210 > 17 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 17 Glu Ala Ala Ala Lys 1 5 < 210 > 18 < 211 > 12 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 18 Cys Arg Arg Arg Arg Arg Arg Glu Ala Glu Ala Cys 1 5 10 < 210 > 19 < 211 > 46 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 19 Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 1 5 10 15 Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 20 25 30 Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 35 40 45 < 210 > 20 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 20 Gly Gly Gly Gly Gly Gly Gly Gly 1 5 < 210 > 21 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 21 Gly Gly Gly Gly Gly Gly 1 5 < 210 > 22 < 211 > 13 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 22 Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Ala Lys Ala 1 5 10 < 210 > 23 < 211 > 5 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 23 Pro Ala Pro Ala Pro 1 5 < 210 > 24 < 211 > 17 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 24 Val Ser Gln Thr Ser Lys Leu Thr Arg Ala Glu Thr Val Phe Pro Asp 1 5 10 15 Val < 210 > 25 < 211 > 6 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 25 Pro Leu Gly Leu Trp Ala 1 5 < 210 > 26 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 26 Thr Arg His Arg Gln Pro Arg Gly Trp Glu 1 5 10 < 210 > 27 < 211 > 10 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 27 Ala Gly Asn Arg Val Arg Arg Ser Val Gly 1 5 10 < 210 > 28 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 28 Arg Arg Arg Arg Arg Arg Arg Arg 1 5 < 210 > 29 < 211 > 4 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 29 Gly Phe Leu Gly 1 < 210 > 30 < 211 > 31 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 30 Gly Ser Ser Gly Gly Ser Gly Ser Ser Gly Gly Ser Gly Gly Gly Asp 1 5 10 15 Glu Ala Asp Gly Ser Arg Gly Ser Gln Lys Ala Gly Val Asp Glu 20 25 30 < 210 > 31 < 211 > 30 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 31 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 1 5 10 15 Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 20 25 30 < 210 > 32 < 211 > 25 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > tPA signal peptide < 400 > 32 Met Asp Ala Met Leu Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 1 5 10 15 Ala Val Phe Val Ser Pro Ser His Ala 20 25 < 210 > 33 < 211 > 180 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > FceRIa < 400 > 33 Val Pro Gln Lys Pro Lys Val Ser Leu Asn Pro Pro Trp Asn Arg Ile 1 5 10 15 Phe Lys Gly Glu Asn Val Thr Leu Thr Cys Asn Gly Asn Asn Phe Phe 20 25 30 Glu Val Ser Ser Thr Lys Trp Phe His Asn Gly Ser Leu Ser Glu Glu 35 40 45 Thr Asn Ser Ser Leu Asn Ile Val Asn Ala Lys Phe Glu Asp Ser Gly 50 55 60 Glu Tyr Lys Cys Gln His Gln Gln Val Asn Glu Ser Glu Pro Val Tyr 65 70 75 80 Leu Glu Val Phe Ser Asp Trp Leu Leu Leu Gln Ala Ser Ala Glu Val 85 90 95 Val Met Glu Gly Gln Pro Leu Phe Leu Arg Cys His Gly Trp Arg Asn 100 105 110 Trp Asp Val Tyr Lys Val Ile Tyr Tyr Lys Asp Gly Glu Ala Leu Lys 115 120 125 Tyr Trp Tyr Glu Asn His Asn Ile Ser Ile Thr Asn Ala Thr Val Glu 130 135 140 Asp Ser Gly Thr Tyr Tyr Cys Thr Gly Lys Val Trp Gln Leu Asp Tyr 145 150 155 160 Glu Ser Glu Pro Leu Asn Ile Thr Val Ile Lys Ala Pro Arg Glu Lys 165 170 175 Tyr Trp Leu Gln 180 < 210 > 34 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 34 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 15 < 210 > 35 < 211 > 33 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 35 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 1 5 10 15 Lys Glu Lys Glu Glu Gln Glu Glu Arg Thr His Thr Cys Pro Pro Cys 20 25 30 Pro < 210 > 36 < 211 > 30 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 36 Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Gly Ser Lys Glu Lys 1 5 10 15 Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro 20 25 30 < 210 > 37 < 211 > 30 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 37 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 1 5 10 15 Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 20 25 30 < 210 > 38 < 211 > 26 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 38 Gly Ser Gly Gly Gly Ser Gly Thr Leu Val Thr Val Ser Ser Glu Ser 1 5 10 15 Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 20 25 < 210 > 39 < 211 > 169 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > monomeric IL - 10 I 87 A variant < 400 > 39 Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 1 5 10 15 Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 20 25 30 Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 35 40 45 Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 50 55 60 Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 65 70 75 80 Glu Asn Gln Asp Pro Asp Ala Lys Ala His Val Asn Ser Leu Gly Glu 85 90 95 Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 100 105 110 Pro Cys Glu Asn Gly Gly Ser Gly Gly Ser Gly Gly Ser Lys Ser Lys 115 120 125 Ala Val Glu Gln Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly 130 135 140 Ile Tyr Lys Ala Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu 145 150 155 160 Ala Tyr Met Thr Met Lys Ile Arg Asn 165 < 210 > 40 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 40 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15 < 210 > 41 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 41 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15 < 210 > 42 < 211 > 8 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 42 Thr His Thr Cys Pro Pro Cys Pro 1 5 < 210 > 43 < 211 > 55 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 43 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala 1 5 10 15 Lys Asn Thr Thr Ala Pro Ala Thr Thr Arg Asn Thr Thr Arg Gly Gly 20 25 30 Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu Glu Gln Glu Glu Arg Thr 35 40 45 His Thr Cys Pro Pro Cys Pro 50 55 < 210 > 44 < 211 > 19 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 44 Ala Gly Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 1 5 10 15 Gly Gly Ser < 210 > 45 < 211 > 15 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > linker peptide < 400 > 45 Gly Gly Gly Ser Gly Gly Ser Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15 < 210 > 46 < 211 > 160 < 212 > PRT < 213 > Artificial Sequence < 220 > < 223 > wild type human IL - 10 ( mature form ) < 400 > 46 Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His Phe Pro 1 5 10 15 Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe Ser Arg 20 25 30 Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu Leu Leu 35 40 45 Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys Gln Ala 50 55 60 Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro Gln Ala 65 70 75 80 Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu Gly Glu 85 90 95 Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg Phe Leu 100 105 110 Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn Ala Phe 115 120 125 Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu Phe Asp 130 135 140 Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile Arg Asn 145 150 155 160